AT4G01970

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002202
Physical Location & Seq
Reverse (-)
853922 .. 857401
3480 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G01970.2

Sequence Viewer

Length: 2631 bp
ATGGCTCCACTTCACGAATCTCTGTCATCAATCAACGATGTAATCGAATCCAAACCTCTTTTTGTTCCAATAACTAAGCCAATCTTGCAGCCAAACTCATTTAACCTATCCGAAGGGTCTCTCTGTGCCAAAGACTCTACTCCAATCCTCTTTGATGTTCCTCAAAACGTAACTTTCACACCATTCTCATCTCACTCCATATCCACTGATGCTCCTCTCCCAATCCTCCTCCGAGTTCAAGCCAACGCTCACAAAGGAGGGTTTCTCGGATTCACCAAAGAATCACCCTCTGATCGTCTCACAAACTCCTTGGGAAGATTCGAAGACAGAGAGTTTCTCAGTCTTTTCCGGTTCAAAATGTGGTGGTCGACGGCTTGGATCGGGAAATCAGGGTCGGATCTTCAAGCCGAGACTCAGTGGGTGATGCTAAAGATTCCTGAGATCGATTCCTACGTGGCCATCATACCAACCATTGAAGGAGCTTTTAGGGCTTCACTTACTCCTGGTGAGAAAGGTAATGTCTTGATTTGTGCAGAGAGTGGCTCAACTAAAGTAAAAGAGTCATCTTTCAAATCCATTGCCTACATTCACATTTGTGACAACCCCTATAACCTCATGAAAGAGGCTTTTAGTGCACTTCGTGTTCATATGAACACTTTCAAGCTTCTAGAAGAGAAGAAGCTTCCGAAAATCGTCGATAAGTTTGGATGGTGCACTTGGGATGCTTGCTACTTGACCGTTGATCCTGCAACCATTTGGACTGGAGTTAAGGAGTTTGAAGATGGTGGGGTGTGTCCGAAGTTTGTCATCATTGATGATGGATGGCAAAGTATTAACTTTGATGGTGATGAGCTAGACAAAGACGCTGAGAATCTTGTTCTTGGTGGGGAGCAAATGACTGCGAGGCTTACCAGCTTCAAGGAGTGTAAGAAGTTTAGAAACTATAAAGGTGGATCCTTTATAACATCTGATGCATCTCACTTCAATCCTCTCAAGCCGAAGATGCTTATCTACAAAGCGACCGAGCGAATTCAGGCGATTATATTGAGACGGAAGTTGGTTAAGGAGTCTGGGGAACAAGATCTGACCGAACTTGATGAAAAGATCAAGATTTTGAGTGAAGAACTCAATGCAATGTTTGATGAGGTTGAGAAAGAAGAGTCTTTGGGTTCTGATGATGTTTCTGGCTCAGGTATGGCGGCTTTCACAAAGGATTTGAGGTTAAGGTTTAAGAGTTTAGATGATATATATGTGTGGCATGCTCTTTGTGGTGCTTGGAATGGTGTTAGGCCTGAAACCATGATGGATTTGAAGGCCAAGGTTGCTCCTTTTGAGCTTTCACCTAGTCTAGGCGCTACGATGGCTGATCTCGCAGTCGACAAGGTTGTAGAAGCTGGGATTGGTCTTGTTCATCCTTCTAAAGCTCATGAATTCTACGACTCGATGCACTCTTATCTTGCTAGTGTTGGTGTTACAGGAGCCAAGATTGATGTTTTCCAAACCTTGGAGTCACTAGCAGAAGAACATGGAGGACGAGTGGAGCTTGCTAAAGCTTACTACGATGGTCTGACCGAATCCATGATCAAGAATTTTAATGGAACTGATGTCATTGCGAGTATGCAACAGTGCAATGAGTTCTTCTTCCTTGCCACAAAGCAAATCTCTATTGGCAGAGTTGGTGATGATTTTTGGTGGCAAGACCCATATGGTGACCCACAAGGAGTATATTGGCTACAAGGAGTTCACATGATTCACTGTTCTTACAACAGTATTTGGATGGGTCAAATGATTCAACCAGATTGGGACATGTTCCAATCAGACCATGTCTGTGCCGAGTACCACGCCGCATCTCGAGCCATTTGCGGTGGGCCTGTCTATCTCAGTGACCATCTTGGCAAAGCCTCACATAACTTTGATCTCATCAAGAAACTTGCTTTCTTTGATGGTACCATCCCAAGGTGTGTCCACTATGCTCTTCCCACTAGAGATTCGCTCTTCAAGAACCCTTTGTTTGACAAAGAGTCAATCCTCAAGATCTTCAACTTCAACAAGTTTGGAGGAGTGATTGGAACATTTAACTGTCAAGGTGCTGGGTGGAGCCCAGAGGAGCATAGGTTCAAAGGATACAAAGAATGTTACACGACAGTTTCGGGAACAGTTCATGTCTCGGACATTGAATGGGATCAGAATCCAGAAGCTGCAGGCTCTCAGGTGACCTACACTGGAGATTACTTGGTTTACAAGCAACAATCTGAAGAAATCCTTTTCATGAACTCGAAATCAGAAGCGATGAAGATAACCCTTGAGCCATCCGCGTTTGACCTGTTGAGTTTCGTGCCAGTCACGGAACTTGTAAGTTCAGGGGTTAGATTCGCACCTCTAGGGTTGATCAACATGTTCAACTGCGTCGGAACAGTCCAAGACATGAAGGTTACTGGTGATAACAGCATAAGAGTGGACGTGAAAGGCGAAGGAAGATTCATGGCGTATTCGAGTTCAGCTCCGGTGAAGTGTTACTTGAACGACAAGGAAGCTGAGTTTAAGTGGGAAGAAGAAACTGGTAAACTCAGTTTCTTTGTTCCTTGGGTTGAAGAATCTGGTGGCATCTCTCATCTGTCTTTCACCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

876

Amino Acids

98.01

Weight (kDa)

5.17

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 81 - 907 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 962
Acc65I GGTACC 1 cut(s) 1946
AccB1I GGYRCC 1 cut(s) 1946
AccB7I CCANNNNNTGG 1 cut(s) 1504
AccI GTMKAC 2 cut(s) 368, 1377
AccII CGCG 1 cut(s) 2315
AciI CCGC 4 cut(s) 1199, 1845, 1863, 2313
AclWI GGATC 6 cut(s) 386, 405, 737, 948, 961, 2190
AcoI YGGCCR 1 cut(s) 456
AcsI RAATTY 3 cut(s) 1029, 1430, 1588
AcuI CTGAAG 1 cut(s) 2274
AdeI CACNNNGTG 1 cut(s) 641
AfaI GTAC 2 cut(s) 1838, 1948
AfiI CCNNNNNNNGG 3 cut(s) 1349, 1504, 1956
AflIII ACRYGT 2 cut(s) 1806, 2394
AhdI GACNNNNNGTC 1 cut(s) 2020
AjiI CACGTC 1 cut(s) 2461
AjnI CCWGG 1 cut(s) 502
AjuI GAANNNNNNNTTGG 2 cut(s) 1383, 1415
AleI CACNNNNGTG 1 cut(s) 594
AloI GAACNNNNNNTCC 2 cut(s) 2099, 2131
Alw21I GWGCWC 2 cut(s) 637, 716
Alw26I GTCTC 5 cut(s) 123, 302, 404, 1042, 2170
Alw44I GTGCAC 2 cut(s) 633, 712
AlwI GGATC 6 cut(s) 386, 405, 737, 948, 961, 2190
AlwNI CAGNNNCTG 1 cut(s) 2198
Ama87I CYCGRG 1 cut(s) 1851
AoxI GGCC 4 cut(s) 456, 1289, 1314, 1868
ApaLI GTGCAC 2 cut(s) 633, 712
ApeKI GCWGC 2 cut(s) 88, 2198
ApoI RAATTY 3 cut(s) 1029, 1430, 1588
Asp700I GAANNNNTTC 1 cut(s) 656
Asp718I GGTACC 1 cut(s) 1946
AspLEI GCGC 1 cut(s) 1355
AspS9I GGNCC 1 cut(s) 1868
AsuII TTCGAA 1 cut(s) 321
AvaI CYCGRG 1 cut(s) 1851
BaeGI GKGCMC 2 cut(s) 637, 716
BalI TGGCCA 1 cut(s) 458
BamHI GGATCC 1 cut(s) 953
BanI GGYRCC 1 cut(s) 1946
BanII GRGCYC 1 cut(s) 2102
BbsI GAAGAC 1 cut(s) 330
Bbv12I GWGCWC 2 cut(s) 637, 716
BbvI GCAGC 2 cut(s) 100, 2185
BceAI ACGGC 1 cut(s) 387
BcgI CGANNNNNNTGC 2 cut(s) 1842, 1876
BciT130I CCWGG 1 cut(s) 504
BciVI GTATCC 1 cut(s) 2117
BclI TGATCA 2 cut(s) 1581, 2388
BcoDI GTCTC 5 cut(s) 123, 302, 404, 1042, 2170
BfaI CTAG 8 cut(s) 668, 854, 1344, 1349, 1461, 1514, 1983, 2381
BfmI CTRYAG 1 cut(s) 2199
BfoI RGCGCY 1 cut(s) 1356
BfuI GTATCC 1 cut(s) 2117
BglII AGATCT 2 cut(s) 1081, 2034
BisI GCNGC 4 cut(s) 89, 1200, 1845, 2199
BlsI GCNGC 4 cut(s) 90, 1201, 1846, 2200
Bme1390I CCNGG 1 cut(s) 504
BmeRI GACNNNNNGTC 1 cut(s) 2020
BmeT110I CYCGRG 1 cut(s) 1851
BmgBI CACGTC 1 cut(s) 2461
BmgT120I GGNCC 1 cut(s) 1868
BmiI GGNNCC 5 cut(s) 6, 955, 1480, 1948, 2099
BmrFI CCNGG 1 cut(s) 504
BmsI GCATC 9 cut(s) 199, 414, 712, 961, 983, 993, 1434, 1856, 2613
BpiI GAAGAC 1 cut(s) 330
BpmI CTGGAG 2 cut(s) 783, 2244
Bpu10I CCTNAGC 1 cut(s) 1189
Bpu14I TTCGAA 1 cut(s) 321
BpuEI CTTGAG 3 cut(s) 977, 2015, 2324
Bsa29I ATCGAT 1 cut(s) 444
BsaAI YACGTR 1 cut(s) 454
BsaBI GATNNNNATC 2 cut(s) 1007, 2187
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 5 cut(s) 309, 1317, 1503, 1955, 2582
BsaWI WCCGGW 2 cut(s) 348, 2503
BsaXI ACNNNNNCTCC 6 cut(s) 196, 226, 1521, 1551, 2099, 2129
Bsc4I CCNNNNNNNGG 3 cut(s) 1349, 1504, 1956
Bse1I ACTGG 5 cut(s) 766, 2227, 2339, 2440, 2563
Bse3DI GCAATG 4 cut(s) 576, 1140, 1608, 1636
Bse8I GATNNNNATC 2 cut(s) 1007, 2187
BseBI CCWGG 1 cut(s) 504
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 5 cut(s) 309, 1317, 1503, 1955, 2582
BseGI GGATG 7 cut(s) 713, 727, 827, 1411, 1782, 1950, 2309
BseJI GATNNNNATC 2 cut(s) 1007, 2187
BseLI CCNNNNNNNGG 3 cut(s) 1349, 1504, 1956
BseMI GCAATG 4 cut(s) 576, 1140, 1608, 1636
BseMII CTCAG 9 cut(s) 352, 428, 429, 858, 1203, 1894, 2222, 2526, 2581
BseNI ACTGG 5 cut(s) 766, 2227, 2339, 2440, 2563
BseRI GAGGAG 4 cut(s) 204, 218, 2073, 2120
BseSI GKGCMC 2 cut(s) 637, 716
BseXI GCAGC 2 cut(s) 100, 2185
BseYI CCCAGC 2 cut(s) 1394, 2090
BsgI GTGCAG 1 cut(s) 552
Bsh1236I CGCG 1 cut(s) 2315
Bsh1285I CGRYCG 1 cut(s) 1023
BshFI GGCC 4 cut(s) 458, 1291, 1316, 1870
BshNI GGYRCC 1 cut(s) 1946
BshVI ATCGAT 1 cut(s) 444
BsiEI CGRYCG 1 cut(s) 1023
BsiHKAI GWGCWC 2 cut(s) 637, 716
BsiHKCI CYCGRG 1 cut(s) 1851
BsiSI CCGG 2 cut(s) 349, 2504
BslFI GGGAC 1 cut(s) 1817
BslI CCNNNNNNNGG 3 cut(s) 1349, 1504, 1956
BsmAI GTCTC 5 cut(s) 123, 302, 404, 1042, 2170
BsmBI CGTCTC 2 cut(s) 302, 1042
BsmFI GGGAC 1 cut(s) 1817
BsnI GGCC 4 cut(s) 458, 1291, 1316, 1870
Bso31I GGTCTC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 1851
Bsp119I TTCGAA 1 cut(s) 321
Bsp1286I GDGCHC 3 cut(s) 637, 716, 2102
BspACI CCGC 4 cut(s) 1199, 1845, 1863, 2313
BspANI GGCC 4 cut(s) 458, 1291, 1316, 1870
BspCNI CTCAG 9 cut(s) 351, 427, 430, 859, 1202, 1893, 2221, 2527, 2580
BspDI ATCGAT 1 cut(s) 444
BspFNI CGCG 1 cut(s) 2315
BspHI TCATGA 3 cut(s) 615, 1426, 2268
BspLI GGNNCC 5 cut(s) 6, 955, 1480, 1948, 2099
BspMAI CTGCAG 1 cut(s) 2203
BspPI GGATC 6 cut(s) 386, 405, 737, 948, 961, 2190
BspQI GCTCTTC 2 cut(s) 1980, 2000
BspT104I TTCGAA 1 cut(s) 321
BspT107I GGYRCC 1 cut(s) 1946
BspTNI GGTCTC 1 cut(s) 123
BsrDI GCAATG 4 cut(s) 576, 1140, 1608, 1636
BsrI ACTGG 5 cut(s) 766, 2227, 2339, 2440, 2563
BssECI CCNNGG 5 cut(s) 309, 1317, 1503, 1955, 2582
BssT1I CCWWGG 5 cut(s) 309, 1317, 1503, 1955, 2582
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 8 cut(s) 739, 1626, 1757, 1769, 2081, 2146, 2158, 2416
Bst6I CTCTTC 4 cut(s) 666, 1152, 1980, 2000
BstBAI YACGTR 1 cut(s) 454
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 4 cut(s) 727, 1260, 1545, 2203
BstEII GGTNACC 2 cut(s) 1709, 2212
BstENI CCTNNNNNAGG 1 cut(s) 1347
BstF5I GGATG 7 cut(s) 713, 727, 827, 1411, 1782, 1950, 2309
BstFNI CGCG 1 cut(s) 2315
BstH2I RGCGCY 1 cut(s) 1356
BstHHI GCGC 1 cut(s) 1355
BstMAI GTCTC 5 cut(s) 123, 302, 404, 1042, 2170
BstMCI CGRYCG 1 cut(s) 1023
BstMWI GCNNNNNNNGC 8 cut(s) 85, 488, 632, 1003, 1322, 1361, 1370, 1853
BstNI CCWGG 1 cut(s) 504
BstNSI RCATGY 3 cut(s) 1262, 1810, 2398
BstPI GGTNACC 2 cut(s) 1709, 2212
BstSCI CCNGG 1 cut(s) 502
BstSFI CTRYAG 1 cut(s) 2199
BstSLI GKGCMC 2 cut(s) 637, 716
BstUI CGCG 1 cut(s) 2315
BstV1I GCAGC 2 cut(s) 100, 2185
BstV2I GAAGAC 1 cut(s) 330
BstX2I RGATCY 4 cut(s) 397, 953, 1081, 2034
BstYI RGATCY 4 cut(s) 397, 953, 1081, 2034
Bsu15I ATCGAT 1 cut(s) 444
BsuI GTATCC 1 cut(s) 2117
BsuRI GGCC 4 cut(s) 458, 1291, 1316, 1870
BsuTUI ATCGAT 1 cut(s) 444
BtgZI GCGATG 1 cut(s) 2303
BtrI CACGTC 1 cut(s) 2461
BtsCI GGATG 7 cut(s) 713, 727, 827, 1411, 1782, 1950, 2309
BtsIMutI CAGTG 6 cut(s) 204, 422, 1631, 1753, 1888, 2220
Cac8I GCNNGC 4 cut(s) 727, 1260, 1545, 2203
CaiI CAGNNNCTG 1 cut(s) 2198
CciI TCATGA 3 cut(s) 615, 1426, 2268
CfoI GCGC 1 cut(s) 1355
Cfr13I GGNCC 1 cut(s) 1868
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 2 cut(s) 872, 2395
Csp6I GTAC 2 cut(s) 1837, 1947
CviQI GTAC 2 cut(s) 1837, 1947
DraIII CACNNNGTG 1 cut(s) 641
DriI GACNNNNNGTC 1 cut(s) 2020
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 4 cut(s) 666, 1152, 1980, 2000
Eam1105I GACNNNNNGTC 1 cut(s) 2020
EarI CTCTTC 4 cut(s) 666, 1152, 1980, 2000
Eco130I CCWWGG 5 cut(s) 309, 1317, 1503, 1955, 2582
Eco147I AGGCCT 1 cut(s) 1291
Eco24I GRGCYC 1 cut(s) 2102
Eco31I GGTCTC 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 2274
Eco88I CYCGRG 1 cut(s) 1851
Eco91I GGTNACC 2 cut(s) 1709, 2212
EcoNI CCTNNNNNAGG 1 cut(s) 1347
EcoO65I GGTNACC 2 cut(s) 1709, 2212
EcoRI GAATTC 2 cut(s) 1029, 1430
EcoRII CCWGG 1 cut(s) 502
EcoT14I CCWWGG 5 cut(s) 309, 1317, 1503, 1955, 2582
EcoT22I ATGCAT 1 cut(s) 976
EcoT38I GRGCYC 1 cut(s) 2102
ErhI CCWWGG 5 cut(s) 309, 1317, 1503, 1955, 2582
Esp3I CGTCTC 2 cut(s) 302, 1042
FalI AAGNNNNNCTT 6 cut(s) 68, 100, 2247, 2279, 2501, 2533
FaqI GGGAC 1 cut(s) 1817
FauNDI CATATG 2 cut(s) 648, 1705
FbaI TGATCA 2 cut(s) 1581, 2388
FblI GTMKAC 2 cut(s) 368, 1377
Fnu4HI GCNGC 4 cut(s) 89, 1200, 1845, 2199
FokI GGATG 7 cut(s) 720, 734, 834, 1398, 1789, 1937, 2296
FriOI GRGCYC 1 cut(s) 2102
Fsp4HI GCNGC 4 cut(s) 89, 1200, 1845, 2199
FspBI CTAG 8 cut(s) 668, 854, 1344, 1349, 1461, 1514, 1983, 2381
GlaI GCGC 1 cut(s) 1354
GluI GCNGC 4 cut(s) 89, 1200, 1845, 2199
GsaI CCCAGC 2 cut(s) 1398, 2094
GsuI CTGGAG 2 cut(s) 783, 2244
HaeII RGCGCY 1 cut(s) 1356
HaeIII GGCC 4 cut(s) 458, 1291, 1316, 1870
HapII CCGG 2 cut(s) 349, 2504
HgaI GACGC 2 cut(s) 872, 2395
HhaI GCGC 1 cut(s) 1355
Hin6I GCGC 1 cut(s) 1353
HinP1I GCGC 1 cut(s) 1353
HincII GTYRAC 2 cut(s) 369, 1378
HindII GTYRAC 2 cut(s) 369, 1378
HindIII AAGCTT 3 cut(s) 662, 680, 1551
HpaII CCGG 2 cut(s) 349, 2504
Hpy166II GTNNAC 9 cut(s) 369, 635, 714, 1378, 1744, 1966, 2239, 2458, 2564
Hpy8I GTNNAC 9 cut(s) 369, 635, 714, 1378, 1744, 1966, 2239, 2458, 2564
Hpy99I CGWCG 3 cut(s) 373, 698, 2411
HpyAV CCTTC 6 cut(s) 107, 470, 1306, 1425, 2422, 2465
HpyCH4III ACNGT 8 cut(s) 739, 1626, 1757, 1769, 2081, 2146, 2158, 2416
HpyCH4IV ACGT 3 cut(s) 168, 453, 2460
HpyF10VI GCNNNNNNNGC 8 cut(s) 85, 488, 632, 1003, 1322, 1361, 1370, 1853
HpySE526I ACGT 3 cut(s) 168, 453, 2460
HspAI GCGC 1 cut(s) 1353
KpnI GGTACC 1 cut(s) 1950
Ksp22I TGATCA 2 cut(s) 1581, 2388
LguI GCTCTTC 2 cut(s) 1980, 2000
Lsp1109I GCAGC 2 cut(s) 100, 2185
LweI GCATC 9 cut(s) 199, 414, 712, 961, 983, 993, 1434, 1856, 2613
MaeI CTAG 8 cut(s) 668, 854, 1344, 1349, 1461, 1514, 1983, 2381
MaeII ACGT 3 cut(s) 168, 453, 2460
MflI RGATCY 4 cut(s) 397, 953, 1081, 2034
MhlI GDGCHC 3 cut(s) 637, 716, 2102
MlsI TGGCCA 1 cut(s) 458
MluCI AATT 3 cut(s) 1029, 1430, 1588
MluNI TGGCCA 1 cut(s) 458
MlyI GAGTC 8 cut(s) 128, 406, 569, 1076, 1169, 1433, 1517, 2030
MmeI TCCRAC 2 cut(s) 375, 2389
Mox20I TGGCCA 1 cut(s) 458
Mph1103I ATGCAT 1 cut(s) 976
MroXI GAANNNNTTC 1 cut(s) 656
MscI TGGCCA 1 cut(s) 458
MslI CAYNNNNRTG 3 cut(s) 594, 1827, 2453
Msp20I TGGCCA 1 cut(s) 458
MspI CCGG 2 cut(s) 349, 2504
MspR9I CCNGG 1 cut(s) 504
MvaI CCWGG 1 cut(s) 504
MvnI CGCG 1 cut(s) 2315
MwoI GCNNNNNNNGC 8 cut(s) 85, 488, 632, 1003, 1322, 1361, 1370, 1853
NdeI CATATG 2 cut(s) 648, 1705
NlaIV GGNNCC 5 cut(s) 6, 955, 1480, 1948, 2099
NmeAIII GCCGAG 2 cut(s) 433, 1858
NmuCI GTSAC 6 cut(s) 596, 1509, 1709, 1883, 2212, 2341
NsiI ATGCAT 1 cut(s) 976
NspI RCATGY 3 cut(s) 1262, 1810, 2398
NspV TTCGAA 1 cut(s) 321
OliI CACNNNNGTG 1 cut(s) 594
PaeI GCATGC 1 cut(s) 1262
PaeR7I CTCGAG 1 cut(s) 1851
PagI TCATGA 3 cut(s) 615, 1426, 2268
PceI AGGCCT 1 cut(s) 1291
PciI ACATGT 2 cut(s) 1806, 2394
PciSI GCTCTTC 2 cut(s) 1980, 2000
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 450, 2466
PdmI GAANNNNTTC 1 cut(s) 656
PflFI GACNNNGTC 1 cut(s) 1823
PflMI CCANNNNNTGG 1 cut(s) 1504
PkrI GCNGC 4 cut(s) 90, 1201, 1846, 2200
PleI GAGTC 8 cut(s) 128, 406, 568, 1075, 1168, 1433, 1516, 2029
PpsI GAGTC 8 cut(s) 128, 406, 568, 1075, 1168, 1433, 1516, 2029
Ppu21I YACGTR 1 cut(s) 454
PscI ACATGT 2 cut(s) 1806, 2394
PsiI TTATAA 1 cut(s) 962
Psp6I CCWGG 1 cut(s) 502
PspEI GGTNACC 2 cut(s) 1709, 2212
PspFI CCCAGC 2 cut(s) 1394, 2090
PspGI CCWGG 1 cut(s) 502
PspN4I GGNNCC 5 cut(s) 6, 955, 1480, 1948, 2099
PspPI GGNCC 1 cut(s) 1868
PstI CTGCAG 1 cut(s) 2203
PstNI CAGNNNCTG 1 cut(s) 2198
PsuI RGATCY 4 cut(s) 397, 953, 1081, 2034
PsyI GACNNNGTC 1 cut(s) 1823
RsaI GTAC 2 cut(s) 1838, 1948
RsaNI GTAC 2 cut(s) 1837, 1947
RseI CAYNNNNRTG 3 cut(s) 594, 1827, 2453
SalI GTCGAC 2 cut(s) 367, 1376
SapI GCTCTTC 2 cut(s) 1980, 2000
SatI GCNGC 4 cut(s) 89, 1200, 1845, 2199
Sau96I GGNCC 1 cut(s) 1868
SchI GAGTC 8 cut(s) 128, 406, 569, 1076, 1169, 1433, 1517, 2030
ScrFI CCNGG 1 cut(s) 504
SduI GDGCHC 3 cut(s) 637, 716, 2102
SfaNI GCATC 9 cut(s) 199, 414, 712, 961, 983, 993, 1434, 1856, 2613
SfcI CTRYAG 1 cut(s) 2199
Sfr274I CTCGAG 1 cut(s) 1851
SfuI TTCGAA 1 cut(s) 321
SlaI CTCGAG 1 cut(s) 1851
SmiMI CAYNNNNRTG 3 cut(s) 594, 1827, 2453
SmlI CTYRAG 4 cut(s) 992, 1851, 2030, 2303
SmoI CTYRAG 4 cut(s) 992, 1851, 2030, 2303
SphI GCATGC 1 cut(s) 1262
Sse9I AATT 3 cut(s) 1029, 1430, 1588
SseBI AGGCCT 1 cut(s) 1291
SsiI CCGC 4 cut(s) 1199, 1845, 1863, 2313
SspMI CTAG 8 cut(s) 668, 854, 1344, 1349, 1461, 1514, 1983, 2381
StuI AGGCCT 1 cut(s) 1291
StyD4I CCNGG 1 cut(s) 502
StyI CCWWGG 5 cut(s) 309, 1317, 1503, 1955, 2582
TaaI ACNGT 8 cut(s) 739, 1626, 1757, 1769, 2081, 2146, 2158, 2416
TaiI ACGT 3 cut(s) 171, 456, 2463
TaqII GACCGA 3 cut(s) 1037, 1103, 1586
TasI AATT 3 cut(s) 1029, 1430, 1588
TauI GCSGC 2 cut(s) 1202, 1847
TscAI CASTG 6 cut(s) 211, 422, 1631, 1760, 1888, 2227
TseFI GTSAC 6 cut(s) 596, 1509, 1709, 1883, 2212, 2341
TseI GCWGC 2 cut(s) 88, 2198
Tsp45I GTSAC 6 cut(s) 596, 1509, 1709, 1883, 2212, 2341
TspGWI ACGGA 2 cut(s) 1066, 2360
TspRI CASTG 6 cut(s) 211, 422, 1631, 1760, 1888, 2227
Tth111I GACNNNGTC 1 cut(s) 1823
Van91I CCANNNNNTGG 1 cut(s) 1504
VneI GTGCAC 2 cut(s) 633, 712
XagI CCTNNNNNAGG 1 cut(s) 1347
XapI RAATTY 3 cut(s) 1029, 1430, 1588
XbaI TCTAGA 1 cut(s) 667
XceI RCATGY 3 cut(s) 1262, 1810, 2398
XhoI CTCGAG 1 cut(s) 1851
XmiI GTMKAC 2 cut(s) 368, 1377
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 8 cut(s) 668, 854, 1344, 1349, 1461, 1514, 1983, 2381
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.