FvH4_1g27000

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002202
Physical Location & Seq
Reverse (-)
18783642 .. 18787143
3502 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27000.t1

Sequence Viewer

Length: 2598 bp
ATGGCACCTCCTAATGATCCTATCAGTCCAGTTTTCAATGCGTTGCAGTCCCAGAGTTCTGGAAAATACTTTGACCTCTCTAATGGAAAGCTCAGTGTCAAAGGTGTTCCATTGCTCTCTGAAGTTCCAAGCAATGTAACTTTCAGCCACTTCCACCCTGCTTACCAATCTTCTGATGCTCCACTTCCTCTGCTCCAACGGGTTCGTGCCTCGTCCTGCAAGGGTGCTTTCCTTGGATTCAACAAGGAGGGTCCCTCGGATAGGCAACTCAACTCTTTGGGGAAACTCATTAACAGAGATTTCCTTAGCATCTTTAGGTTCAAGACATGGTGGTCTACTATGTGGGTGGGAAACTCTGGTTCAAACTTGCAGATGGAAACCCAATGGGTTCTCTTAGACGTCCCAGAGATCAAGTCATATGTTATAATCATACCCATCATTGAAGGCAGTTTCAGGTCTGCTCTGCATCCTGGCAGTGATGACCATGTCATGATCTGCGCTGAGAGTGGCTCTAGTGCAGTGAAAGCATCACATTTCGGTGCCGTTGCTTATGTTCATGTTTCTGATAATCCTTACAACTTGATGAAGGAAGCTTACAGTGCTCTCAGAGTCCATCTCAATACTTTTAGGCTCTTGGAAGAGAAGACAGTGCCAAATCTAGTTAACAAATTTGGTTGGTGCACTTGGGATGCATTCTACTTAGCTGTAGAACCTGTTGGTGTCTGGCATGGTGTGAAAGAATTTTTTGAAGGCGGTGTGTCACCGCGGTTCCTCATCATTGATGATGGTTGGCAGAGCATCAATATGGATGATGAGGACCTCAAGGAGGACACAAAAAATTTAGTTCTTGGTGGGACTCAAATGACTGCTAGGCTTTACAGGTTTGAGGAGTGCAAGAAGTTCAGGAACTACAAAGGTGGGACGATGTTGGGTCCTGATGCCCCTTCCTTTGATCCCAACAAGCCAAAACTGTTGATTGCCAAGGCAATTGAAATAGAGCATGCTGAGAAGGACCGCGACAAGGCCCTTCAATCTGGAATCACCGACTTGTCTTTGTTTGAAACAAAAATTCAGAAGCTGAAGACAGAGTTGAATGAGATCATTGGTGGAGGAGAAAGCAGTGCTAGTAATGAAAGCTGTGGAAGCTGCTCTTGCAGTGACAAAAACTATGGAATGAAGGCTTTCACGGGTGACTTGAGAACCAAGTTTAAAGGTTTAGATGATATATACGTATGGCATGCTCTTTGTGGTGCCTGGGGTGGTGTTAGACCAGGTTCAACTCATCTCAGTTCGAAGATCATTCCTTGCAAAGTCTCTCCTGGACTAGATGGGACGATGACTGATCTCGCTGTGGTGAAAATTGTTGAGGGCGGAATTGGGCTTGTTCATCCTGATCAAGCTGATAGTTTCTACGACTCCTTGCACTCATATCTTTCTGAAGTCGGTATCACTGGAGTCAAAGTGGATGTTATTCATACGCTTGAATATGTATCAGAGGAATATGGAGGCCGTGTGGAGCTTGCAAAAGCTTATTACAAGGGGCTGACTCATTCTCTTCAGAAGAACTTCAATGGGACCGGACTTATTGCTAGCATGCAGCAATGCAACGACTTCTTCTTTCTTGGCACAAAGCAAATATCTATTGGAAGAGTCGGTGACGATTTTTGGTTTCAGGATCCGAATGGAGATCCAATGGGAGTCTACTGGTTACAAGGAGTTCATATGATCCACTGCGCCTACAACAGCATGTGGATGGGTCAGATGATACAGCCTGATTGGGATATGTTTCAATCAGACCATATATGTGCTAAATTTCATGCAGGCTCTAGAGCAATCTGTGGTGGTCCGGTTTATGTGAGTGACTCAGTTTCAGGGCATGATTTCGATCTCATTAAGAAGCTTGTTTTCCCTGATGGTACCATTCCCAAGTGCCAGCATTTTGCCCTCCCAACCAGGGACTGCCTCTTTAAAAACCCTTTATTTGATGATAAGACTGTCCTTAAAATTTGGAACTTCAACAAGTATGGTGGTGTGCTTGGAGCTTTCAATTGTCAAGGGGCTGGTTGGGACCCCAAAGAGCAGAGGATCAAAGGCCACTCAGAATGCTACAAGCCAATCTCTTGTTCGTTACATGTTTCTGAGATCGAATGGGACCAAAAGACTGAAGCAGCACATTTGGGAGAGGCTGAAGAGTATGTTGTCTATCTCACCGAGGCTAAGGAGCTCCGCCTAATGACCCCTAAGTCTGATGCCATTCAAATCGTCATCCAACCATCTTCGTTTGAGCTTTTGAGCTTTGTGCCGGTTAGAAAGCTAGGCCGGAGCATCAACTTCGCGCCAATCGGACTTACAAATATGTTCAACTGTGGAGGCACACTCCAAGAGTTGGAGTACAAAACTACTGCGGTCGAGTCTAGTGCCATGATCAAAGTTAAAGGTGGAGGCCATTTCCTGGCCTACTCAAGTGAGTCACCAAAGAAATGTTGTTTGAATGGTGCGGAGGTAGCTTTTGAGTGGTCTGCTGATGGCAAATTGAACTTGAGTGTCCCTTGGATTGAACAAGCTGCTGGCATTTCTGATGTGCTGTTTGCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

866

Amino Acids

95.74

Weight (kDa)

5.85

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 848 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AasI GACNNNNNNGTC 2 cut(s) 331, 2242
AatII GACGTC 1 cut(s) 402
Acc65I GGTACC 1 cut(s) 1916
AccB1I GGYRCC 4 cut(s) 4, 539, 1250, 1916
AccB7I CCANNNNNTGG 2 cut(s) 2386, 2452
AccI GTMKAC 2 cut(s) 335, 1701
AccII CGCG 3 cut(s) 766, 1017, 2336
AciI CCGC 8 cut(s) 753, 764, 766, 1015, 1371, 2227, 2405, 2498
AclWI GGATC 7 cut(s) 11, 947, 1670, 1682, 1683, 1720, 2093
AcsI RAATTY 6 cut(s) 668, 740, 838, 1068, 1811, 2004
AcuI CTGAAG 6 cut(s) 141, 1100, 1458, 1541, 2184, 2208
AcyI GRCGYC 1 cut(s) 399
AfaI GTAC 2 cut(s) 1918, 2393
AfiI CCNNNNNNNGG 6 cut(s) 261, 826, 1021, 1954, 2386, 2452
AflIII ACRYGT 1 cut(s) 2131
AjnI CCWGG 6 cut(s) 469, 1253, 1270, 1318, 1952, 2451
AjuI GAANNNNNNNTTGG 2 cut(s) 2107, 2139
Alw21I GWGCWC 3 cut(s) 604, 683, 2226
Alw26I GTCTC 1 cut(s) 1318
Alw44I GTGCAC 1 cut(s) 679
AlwI GGATC 7 cut(s) 11, 947, 1670, 1682, 1683, 1720, 2093
AlwNI CAGNNNCTG 2 cut(s) 1078, 1959
AoxI GGCC 6 cut(s) 1023, 1507, 2092, 2317, 2443, 2454
ApaLI GTGCAC 1 cut(s) 679
ApeKI GCWGC 4 cut(s) 1146, 1597, 2168, 2564
ApoI RAATTY 6 cut(s) 668, 740, 838, 1068, 1811, 2004
Asp700I GAANNNNTTC 2 cut(s) 1181, 1565
Asp718I GGTACC 1 cut(s) 1916
AspLEI GCGC 3 cut(s) 500, 1736, 2338
AspS9I GGNCC 9 cut(s) 251, 817, 932, 1012, 1024, 1575, 1844, 2068, 2152
AsuHPI GGTGA 7 cut(s) 753, 1033, 1202, 1366, 1667, 2200, 2463
AsuII TTCGAA 1 cut(s) 1292
AsuNHI GCTAGC 1 cut(s) 1589
AvaII GGWCC 8 cut(s) 251, 817, 932, 1012, 1575, 1844, 2068, 2152
BaeGI GKGCMC 1 cut(s) 683
BamHI GGATCC 1 cut(s) 1675
BanI GGYRCC 4 cut(s) 4, 539, 1250, 1916
BanII GRGCYC 1 cut(s) 2226
BarI GAAGNNNNNNTAC 2 cut(s) 578, 610
BbsI GAAGAC 2 cut(s) 650, 1088
Bbv12I GWGCWC 3 cut(s) 604, 683, 2226
BbvI GCAGC 4 cut(s) 1133, 1609, 2180, 2551
BccI CCATC 9 cut(s) 367, 443, 621, 779, 1322, 1747, 1907, 2281, 2519
BceAI ACGGC 2 cut(s) 527, 1494
BcgI CGANNNNNNTGC 4 cut(s) 2314, 2348, 2399, 2433
BciT130I CCWGG 6 cut(s) 471, 1255, 1272, 1320, 1954, 2453
BclI TGATCA 2 cut(s) 1393, 2424
BcoDI GTCTC 1 cut(s) 1318
BfaI CTAG 9 cut(s) 513, 659, 870, 1125, 1325, 1590, 1827, 2315, 2415
BfmI CTRYAG 1 cut(s) 705
BisI GCNGC 4 cut(s) 1147, 1598, 2169, 2565
BlsI GCNGC 4 cut(s) 1148, 1599, 2170, 2566
Bme1390I CCNGG 6 cut(s) 471, 1255, 1272, 1320, 1954, 2453
Bme18I GGWCC 8 cut(s) 251, 817, 932, 1012, 1575, 1844, 2068, 2152
BmgT120I GGNCC 9 cut(s) 251, 817, 932, 1012, 1024, 1575, 1844, 2068, 2152
BmrFI CCNGG 6 cut(s) 471, 1255, 1272, 1320, 1954, 2453
BmsI GCATC 9 cut(s) 166, 318, 475, 536, 679, 807, 928, 2239, 2334
BmtI GCTAGC 1 cut(s) 1593
BpiI GAAGAC 2 cut(s) 650, 1088
BplI GAGNNNNNCTC 8 cut(s) 239, 271, 494, 526, 600, 632, 2361, 2393
BpmI CTGGAG 1 cut(s) 1473
Bpu10I CCTNAGC 2 cut(s) 305, 2217
Bpu14I TTCGAA 1 cut(s) 1292
BpuEI CTTGAG 4 cut(s) 806, 1216, 2446, 2560
BsaAI YACGTR 1 cut(s) 1231
BsaBI GATNNNNATC 1 cut(s) 1884
BsaHI GRCGYC 1 cut(s) 399
BsaJI CCNNGG 8 cut(s) 232, 255, 764, 981, 1254, 1953, 2211, 2549
BsaWI WCCGGW 2 cut(s) 1577, 1846
BsaXI ACNNNNNCTCC 4 cut(s) 1446, 1476, 1497, 1527
Bsc4I CCNNNNNNNGG 6 cut(s) 261, 826, 1021, 1954, 2386, 2452
Bse118I RCCGGY 1 cut(s) 2302
Bse1I ACTGG 3 cut(s) 29, 1456, 1709
Bse3DI GCAATG 3 cut(s) 110, 139, 1607
Bse8I GATNNNNATC 1 cut(s) 1884
BseBI CCWGG 6 cut(s) 471, 1255, 1272, 1320, 1954, 2453
BseDI CCNNGG 8 cut(s) 232, 255, 764, 981, 1254, 1953, 2211, 2549
BseGI GGATG 7 cut(s) 466, 694, 814, 1387, 1471, 1758, 2265
BseJI GATNNNNATC 1 cut(s) 1884
BseLI CCNNNNNNNGG 6 cut(s) 261, 826, 1021, 1954, 2386, 2452
BseMI GCAATG 3 cut(s) 110, 139, 1607
BseMII CTCAG 8 cut(s) 106, 492, 619, 996, 1300, 1878, 2112, 2130
BseNI ACTGG 3 cut(s) 29, 1456, 1709
BseRI GAGGAG 2 cut(s) 902, 1125
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 4 cut(s) 1133, 1609, 2180, 2551
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 3 cut(s) 766, 1017, 2336
Bsh1285I CGRYCG 1 cut(s) 2409
BshFI GGCC 6 cut(s) 1025, 1509, 2094, 2319, 2445, 2456
BshNI GGYRCC 4 cut(s) 4, 539, 1250, 1916
BsiEI CGRYCG 1 cut(s) 2409
BsiHKAI GWGCWC 3 cut(s) 604, 683, 2226
BsiSI CCGG 4 cut(s) 1578, 1847, 2303, 2320
BslI CCNNNNNNNGG 6 cut(s) 261, 826, 1021, 1954, 2386, 2452
BsmAI GTCTC 1 cut(s) 1318
BsmI GAATGC 2 cut(s) 692, 2108
BsnI GGCC 6 cut(s) 1025, 1509, 2094, 2319, 2445, 2456
Bsp119I TTCGAA 1 cut(s) 1292
Bsp1286I GDGCHC 3 cut(s) 604, 683, 2226
BspACI CCGC 8 cut(s) 753, 764, 766, 1015, 1371, 2227, 2405, 2498
BspANI GGCC 6 cut(s) 1025, 1509, 2094, 2319, 2445, 2456
BspCNI CTCAG 8 cut(s) 105, 493, 618, 997, 1299, 1877, 2111, 2131
BspFNI CGCG 3 cut(s) 766, 1017, 2336
BspHI TCATGA 1 cut(s) 489
BspOI GCTAGC 1 cut(s) 1593
BspPI GGATC 7 cut(s) 11, 947, 1670, 1682, 1683, 1720, 2093
BspT104I TTCGAA 1 cut(s) 1292
BspT107I GGYRCC 4 cut(s) 4, 539, 1250, 1916
BsrDI GCAATG 3 cut(s) 110, 139, 1607
BsrFI RCCGGY 1 cut(s) 2302
BsrI ACTGG 3 cut(s) 29, 1456, 1709
BssAI RCCGGY 1 cut(s) 2302
BssECI CCNNGG 8 cut(s) 232, 255, 764, 981, 1254, 1953, 2211, 2549
BssNI GRCGYC 1 cut(s) 399
BssT1I CCWWGG 3 cut(s) 232, 981, 2549
Bst2UI CCWGG 6 cut(s) 471, 1255, 1272, 1320, 1954, 2453
Bst4CI ACNGT 5 cut(s) 599, 649, 972, 1996, 2366
Bst6I CTCTTC 4 cut(s) 633, 1560, 1642, 2184
BstACI GRCGYC 1 cut(s) 399
BstBAI YACGTR 1 cut(s) 1231
BstBI TTCGAA 1 cut(s) 1292
BstC8I GCNNGC 8 cut(s) 1002, 1239, 1521, 1591, 1595, 1822, 1934, 2569
BstDSI CCRYGG 1 cut(s) 764
BstENI CCTNNNNNAGG 2 cut(s) 259, 824
BstF5I GGATG 7 cut(s) 466, 694, 814, 1387, 1471, 1758, 2265
BstFNI CGCG 3 cut(s) 766, 1017, 2336
BstHHI GCGC 3 cut(s) 500, 1736, 2338
BstMAI GTCTC 1 cut(s) 1318
BstMCI CGRYCG 1 cut(s) 2409
BstMWI GCNNNNNNNGC 5 cut(s) 524, 599, 1143, 1152, 2504
BstNI CCWGG 6 cut(s) 471, 1255, 1272, 1320, 1954, 2453
BstNSI RCATGY 5 cut(s) 1004, 1241, 1597, 1750, 2135
BstSCI CCNGG 6 cut(s) 469, 1253, 1270, 1318, 1952, 2451
BstSFI CTRYAG 1 cut(s) 705
BstSLI GKGCMC 1 cut(s) 683
BstSNI TACGTA 1 cut(s) 1231
BstUI CGCG 3 cut(s) 766, 1017, 2336
BstV1I GCAGC 4 cut(s) 1133, 1609, 2180, 2551
BstV2I GAAGAC 2 cut(s) 650, 1088
BstX2I RGATCY 2 cut(s) 1675, 1687
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 2 cut(s) 1675, 1687
BsuRI GGCC 6 cut(s) 1025, 1509, 2094, 2319, 2445, 2456
BtgI CCRYGG 1 cut(s) 764
BtsCI GGATG 7 cut(s) 466, 694, 814, 1387, 1471, 1758, 2265
BtsI GCAGTG 5 cut(s) 481, 525, 1126, 1162, 1729
BtsIMutI CAGTG 9 cut(s) 100, 481, 525, 604, 654, 1126, 1162, 1449, 1729
Cac8I GCNNGC 8 cut(s) 1002, 1239, 1521, 1591, 1595, 1822, 1934, 2569
CaiI CAGNNNCTG 2 cut(s) 1078, 1959
CciI TCATGA 1 cut(s) 489
CfoI GCGC 3 cut(s) 500, 1736, 2338
Cfr10I RCCGGY 1 cut(s) 2302
Cfr13I GGNCC 9 cut(s) 251, 817, 932, 1012, 1024, 1575, 1844, 2068, 2152
Cfr42I CCGCGG 1 cut(s) 767
CsiI ACCWGGT 1 cut(s) 1270
Csp6I GTAC 2 cut(s) 1917, 2392
CspCI CAANNNNNGTGG 2 cut(s) 2008, 2043
CviQI GTAC 2 cut(s) 1917, 2392
DraI TTTAAA 2 cut(s) 1210, 1969
DrdI GACNNNNNNGTC 2 cut(s) 331, 2242
DseDI GACNNNNNNGTC 2 cut(s) 331, 2242
Eam1104I CTCTTC 4 cut(s) 633, 1560, 1642, 2184
EarI CTCTTC 4 cut(s) 633, 1560, 1642, 2184
EciI GGCGGA 2 cut(s) 1386, 2216
Ecl136II GAGCTC 1 cut(s) 2224
Eco105I TACGTA 1 cut(s) 1231
Eco130I CCWWGG 3 cut(s) 232, 981, 2549
Eco24I GRGCYC 1 cut(s) 2226
Eco47I GGWCC 8 cut(s) 251, 817, 932, 1012, 1575, 1844, 2068, 2152
Eco53kI GAGCTC 1 cut(s) 2224
Eco57I CTGAAG 6 cut(s) 141, 1100, 1458, 1541, 2184, 2208
EcoICRI GAGCTC 1 cut(s) 2224
EcoNI CCTNNNNNAGG 2 cut(s) 259, 824
EcoO109I RGGNCCY 5 cut(s) 251, 817, 932, 1024, 2068
EcoRII CCWGG 6 cut(s) 469, 1253, 1270, 1318, 1952, 2451
EcoT14I CCWWGG 3 cut(s) 232, 981, 2549
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 2226
ErhI CCWWGG 3 cut(s) 232, 981, 2549
FalI AAGNNNNNCTT 2 cut(s) 1135, 1167
FauNDI CATATG 2 cut(s) 418, 1722
FbaI TGATCA 2 cut(s) 1393, 2424
FblI GTMKAC 2 cut(s) 335, 1701
Fnu4HI GCNGC 4 cut(s) 1147, 1598, 2169, 2565
FokI GGATG 7 cut(s) 453, 701, 821, 1374, 1478, 1765, 2252
FriOI GRGCYC 1 cut(s) 2226
Fsp4HI GCNGC 4 cut(s) 1147, 1598, 2169, 2565
FspBI CTAG 9 cut(s) 513, 659, 870, 1125, 1325, 1590, 1827, 2315, 2415
GlaI GCGC 3 cut(s) 499, 1735, 2337
GluI GCNGC 4 cut(s) 1147, 1598, 2169, 2565
GsuI CTGGAG 1 cut(s) 1473
HaeIII GGCC 6 cut(s) 1025, 1509, 2094, 2319, 2445, 2456
HapII CCGG 4 cut(s) 1578, 1847, 2303, 2320
HhaI GCGC 3 cut(s) 500, 1736, 2338
Hin1I GRCGYC 1 cut(s) 399
Hin6I GCGC 3 cut(s) 498, 1734, 2336
HinP1I GCGC 3 cut(s) 498, 1734, 2336
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 3 cut(s) 591, 1527, 1898
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 4 cut(s) 1578, 1847, 2303, 2320
HphI GGTGA 7 cut(s) 753, 1033, 1202, 1366, 1667, 2200, 2463
Hpy166II GTNNAC 4 cut(s) 336, 664, 681, 1702
Hpy188III TCNNGA 9 cut(s) 60, 322, 490, 904, 935, 1035, 1391, 1673, 1827
Hpy8I GTNNAC 4 cut(s) 336, 664, 681, 1702
HpyAV CCTTC 7 cut(s) 437, 580, 743, 954, 1003, 1037, 1171
HpyCH4III ACNGT 5 cut(s) 599, 649, 972, 1996, 2366
HpyCH4IV ACGT 2 cut(s) 399, 1230
HpyF10VI GCNNNNNNNGC 5 cut(s) 524, 599, 1143, 1152, 2504
HpySE526I ACGT 2 cut(s) 399, 1230
Hsp92I GRCGYC 1 cut(s) 399
HspAI GCGC 3 cut(s) 498, 1734, 2336
KflI GGGWCCC 2 cut(s) 251, 2068
KpnI GGTACC 1 cut(s) 1920
Ksp22I TGATCA 2 cut(s) 1393, 2424
KspAI GTTAAC 1 cut(s) 664
KspI CCGCGG 1 cut(s) 767
LmnI GCTCC 7 cut(s) 184, 198, 1516, 2039, 2221, 2229, 2322
Lsp1109I GCAGC 4 cut(s) 1133, 1609, 2180, 2551
LweI GCATC 9 cut(s) 166, 318, 475, 536, 679, 807, 928, 2239, 2334
MabI ACCWGGT 1 cut(s) 1270
MaeI CTAG 9 cut(s) 513, 659, 870, 1125, 1325, 1590, 1827, 2315, 2415
MaeII ACGT 2 cut(s) 399, 1230
MaeIII GTNAC 9 cut(s) 136, 759, 1157, 1190, 1655, 1707, 1859, 2127, 2469
MfeI CAATTG 2 cut(s) 987, 2047
MflI RGATCY 2 cut(s) 1675, 1687
MhlI GDGCHC 3 cut(s) 604, 683, 2226
MmeI TCCRAC 3 cut(s) 220, 2293, 2367
Mph1103I ATGCAT 1 cut(s) 694
MroXI GAANNNNTTC 2 cut(s) 1181, 1565
MseI TTAA 7 cut(s) 291, 663, 1209, 1893, 1968, 2001, 2433
MslI CAYNNNNRTG 4 cut(s) 537, 803, 1751, 1803
MspA1I CMGCKG 1 cut(s) 766
MspI CCGG 4 cut(s) 1578, 1847, 2303, 2320
MspR9I CCNGG 6 cut(s) 471, 1255, 1272, 1320, 1954, 2453
MunI CAATTG 2 cut(s) 987, 2047
Mva1269I GAATGC 2 cut(s) 692, 2108
MvaI CCWGG 6 cut(s) 471, 1255, 1272, 1320, 1954, 2453
MvnI CGCG 3 cut(s) 766, 1017, 2336
MwoI GCNNNNNNNGC 5 cut(s) 524, 599, 1143, 1152, 2504
NdeI CATATG 2 cut(s) 418, 1722
NheI GCTAGC 1 cut(s) 1589
NmuCI GTSAC 6 cut(s) 759, 1157, 1190, 1655, 1859, 2469
NsiI ATGCAT 1 cut(s) 694
NspI RCATGY 5 cut(s) 1004, 1241, 1597, 1750, 2135
NspV TTCGAA 1 cut(s) 1292
PaeI GCATGC 3 cut(s) 1004, 1241, 1597
PagI TCATGA 1 cut(s) 489
PciI ACATGT 1 cut(s) 2131
PctI GAATGC 2 cut(s) 692, 2108
PdmI GAANNNNTTC 2 cut(s) 1181, 1565
PfeI GAWTC 2 cut(s) 237, 1038
PflFI GACNNNGTC 1 cut(s) 485
PflMI CCANNNNNTGG 2 cut(s) 2386, 2452
PfoI TCCNGGA 1 cut(s) 1318
PkrI GCNGC 4 cut(s) 1148, 1599, 2170, 2566
Ppu21I YACGTR 1 cut(s) 1231
PpuMI RGGWCCY 4 cut(s) 251, 817, 932, 2068
PscI ACATGT 1 cut(s) 2131
PsiI TTATAA 1 cut(s) 425
Psp124BI GAGCTC 1 cut(s) 2226
Psp5II RGGWCCY 4 cut(s) 251, 817, 932, 2068
Psp6I CCWGG 6 cut(s) 469, 1253, 1270, 1318, 1952, 2451
PspGI CCWGG 6 cut(s) 469, 1253, 1270, 1318, 1952, 2451
PspPI GGNCC 9 cut(s) 251, 817, 932, 1012, 1024, 1575, 1844, 2068, 2152
PspPPI RGGWCCY 4 cut(s) 251, 817, 932, 2068
PstNI CAGNNNCTG 2 cut(s) 1078, 1959
PsuI RGATCY 2 cut(s) 1675, 1687
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 2 cut(s) 1918, 2393
RsaNI GTAC 2 cut(s) 1917, 2392
RseI CAYNNNNRTG 4 cut(s) 537, 803, 1751, 1803
SacI GAGCTC 1 cut(s) 2226
SacII CCGCGG 1 cut(s) 767
SaqAI TTAA 7 cut(s) 291, 663, 1209, 1893, 1968, 2001, 2433
SatI GCNGC 4 cut(s) 1147, 1598, 2169, 2565
Sau96I GGNCC 9 cut(s) 251, 817, 932, 1012, 1024, 1575, 1844, 2068, 2152
ScrFI CCNGG 6 cut(s) 471, 1255, 1272, 1320, 1954, 2453
SduI GDGCHC 3 cut(s) 604, 683, 2226
SexAI ACCWGGT 1 cut(s) 1270
SfaNI GCATC 9 cut(s) 166, 318, 475, 536, 679, 807, 928, 2239, 2334
SfcI CTRYAG 1 cut(s) 705
Sfr303I CCGCGG 1 cut(s) 767
SfuI TTCGAA 1 cut(s) 1292
SgrBI CCGCGG 1 cut(s) 767
SinI GGWCC 8 cut(s) 251, 817, 932, 1012, 1575, 1844, 2068, 2152
SmiMI CAYNNNNRTG 4 cut(s) 537, 803, 1751, 1803
SmlI CTYRAG 4 cut(s) 821, 1195, 2461, 2539
SmoI CTYRAG 4 cut(s) 821, 1195, 2461, 2539
SnaBI TACGTA 1 cut(s) 1231
SphI GCATGC 3 cut(s) 1004, 1241, 1597
SsiI CCGC 8 cut(s) 753, 764, 766, 1015, 1371, 2227, 2405, 2498
SspMI CTAG 9 cut(s) 513, 659, 870, 1125, 1325, 1590, 1827, 2315, 2415
SstI GAGCTC 1 cut(s) 2226
StyD4I CCNGG 6 cut(s) 469, 1253, 1270, 1318, 1952, 2451
StyI CCWWGG 3 cut(s) 232, 981, 2549
TaaI ACNGT 5 cut(s) 599, 649, 972, 1996, 2366
TaiI ACGT 2 cut(s) 402, 1233
TaqI TCGA 4 cut(s) 1292, 1884, 2145, 2409
TatI WGTACW 1 cut(s) 2391
TfiI GAWTC 2 cut(s) 237, 1038
Tru1I TTAA 7 cut(s) 291, 663, 1209, 1893, 1968, 2001, 2433
Tru9I TTAA 7 cut(s) 291, 663, 1209, 1893, 1968, 2001, 2433
TscAI CASTG 9 cut(s) 100, 481, 525, 604, 654, 1126, 1162, 1456, 1736
TseFI GTSAC 6 cut(s) 759, 1157, 1190, 1655, 1859, 2469
TseI GCWGC 4 cut(s) 1146, 1597, 2168, 2564
Tsp45I GTSAC 6 cut(s) 759, 1157, 1190, 1655, 1859, 2469
TspDTI ATGAA 8 cut(s) 545, 599, 1146, 1190, 1376, 1463, 1709, 1805
TspRI CASTG 9 cut(s) 100, 481, 525, 604, 654, 1126, 1162, 1456, 1736
Tth111I GACNNNGTC 1 cut(s) 485
Van91I CCANNNNNTGG 2 cut(s) 2386, 2452
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 8 cut(s) 251, 817, 932, 1012, 1575, 1844, 2068, 2152
XagI CCTNNNNNAGG 2 cut(s) 259, 824
XapI RAATTY 6 cut(s) 668, 740, 838, 1068, 1811, 2004
XbaI TCTAGA 1 cut(s) 1826
XceI RCATGY 5 cut(s) 1004, 1241, 1597, 1750, 2135
XmiI GTMKAC 2 cut(s) 335, 1701
XmnI GAANNNNTTC 2 cut(s) 1181, 1565
XspI CTAG 9 cut(s) 513, 659, 870, 1125, 1325, 1590, 1827, 2315, 2415
ZraI GACGTC 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.