Rroxscaffold_6G00394970

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002202
Physical Location & Seq
Forward (+)
15824505 .. 15828026
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394970.1

Sequence Viewer

Length: 2598 bp
ATGGCACCTCCTAATGATCCTATTCGTCCAGTTTTCAATGCGTTCCGGTCCGAGAGTTCTGGAAAATACTTTGACCTCTCTAATGGAAAGCTTAGTGTCAAAGGGATTCCATTGCTCTCTGAAGTTCCAAGCAATGTAACTTTCAGTCACTTCCACTCAGCCTGCCAATCTTCTGATGTTCCACTTCCTCTGCTCCAACGGGTTCGTGCCTCGTCCCACAAGGGTGCTTTCCTTGGATTCAACAAGGAGGTTCCCTCAGATAGGCTGCTCAACTCCTTGGGGAAACTCAGTAACAGGGATTTCCTTAGCATCTTTAGGTTCAAGACATGGTGGTCTACTATGTGGGTGGGAAACTCTGGTTCAAACTTGCAAATGGAAACCCAATGGGTGCTCTTAGATGTCCCGGAGATCAAGTCATATGTTATAATCATACCCATCATTGAAGCCAGTTTCAGGTCTGCTCTTCATCCTGGCGGTGATGACCATGTCATGATCTGTGCCGAGAGTGGTTCTAGTCAAGTGAAAGCGTCGAATTTCAATGCCATTGCTTATGTTCATGCTTCTGAAAATCCCTACAACTTGATGAAAGAAGCCTATAGTGCAGTTAGAGTCCATCTCAATAGTTTTAGGCTCTTGGAAGAGAAGACGGTCCCAAATCTAGTTAACAAATTTGGTTGGTGCACTTGGGATGCATTCTACTTAACTGTAGAACCTGTTGGTGTCTGGCATGGTGTGAAAGAATTTGTTGAAGGCGGTGTGTCCCCGCGGTTTCTCATAATTGATGATGGTTGGCAAAGCATCAATATGGATGATGAGGACCTGAAGGAGGATACAAAAAATCTAGTTCTTGGTGGGACTCAAATGACTGCTAGGCTTCACAGTTTTGAGGAGTGCAAAAAGTTCAGGAACTACAGAGGTGGCTCTATGTTGGGTCCTGATGCTCCTTCATTTGATCCCAAAAAGCCAAAACTGTCGATTGCCAAGGCAATTGAAATAGAGCATGCTGAGAAGGAACACGACAAGGCCCTTCATTCTGGAGTCACCGAGTTGTCTGTGTTCGAAACAAAACTTCAGAAGCTGAAGACAGAGTTGAATGATATCATTGGTGAAGGAGAGAGCAGTGCTTCTAATGAATGCCGTGGAAGCAGTTCTGGCAGTGACAAAAACTATGGAATGAAGGCCTTCACAGGTGACTTGAGAACCAAGTTTAAAGGTTTGGATGACATATATGTATGGCATGCTCTTTGTGGTGCGTGGGGTGGTGTTAGGCCAGGTTCAACTTATCTCAGTTCAAAGATCATTCCTTGCACAGTCTCACCTGGACTAGACGGGACGATGAATGATCTGGCTGTGGTGAAAATCGTTGAGGGCGGGATTGGGCTTGTTCATCCTGATCAAGCTGATAGTTTCTATGACTCCATGCACTCTTACCTTTCTGAAGTTGGTATCACTGGAGTCAAAGTGGATGTAATTCATACACTTGAATATGTATCCGAGGAATATGGAGGCCGTGTGGAGCTTGCAAAGGCTTATTACAAGGGGCTGACTCATTCTCTTCAGAAGAACTTCAATGGTACTGGACTTATTGCTAGCATGCAGCAATGCAATGACTTCTTCTTTCTTGGCACAAAGCAAATCTCTATTGGAAGAGTCGGTGACGATTTTTGGTTTCAGGATCCGAATGGAGATCCAATGGGAGTCTACTGGTTACAAGGAGTTCATATGATCCACTGCGCTTACAACAGCATGTGGATGGGCCAGATGATACAGCCTGATTGGGACATGTTTCAATCAGATCATTTATGTGCCAAATTTCATGCAGGATCAAGAGCAATCTGTGGGGGTCCGGTTTATGTAAGTGACTCAGTTTCAGGGCATGATTTTGATCTCATTAAGAAGCTTGTTTACCCGGATGGTACCATTCCCAAGTGCCAGCATTTCGCTCTCCCAACCAGGGACTGCCTCTTCAAAAACCCTTTATTTGATGATAAGACTGTCCTGAAAATTTGGAACTTTAACAAGTATGGCGGTGTCATAGGAGCTTTCAATTGTCAAGGGGCTGGTTGGGACCCCAAGGAACAGAGGATCAAAGGCCACTCAGAGTGCTACAAGCCAATCTCTTGTTCATTACATGTTCGTGAGATTGAATGGGACCAAAAGTCTGAAGCAGCCCATTTGGGCGAGGCTGAAGAGTACGTTGTCTACCTCAGCGAGGTTGATGAGCTCCGCCTGATGACCCCAAAGTCCGATGCAATTCAAATCGTCATCCAGCCATCTTCGTTTGAGATTTTGAGCTTTGTGCCGGTTAGAAAGCTAGGCCACAGCATTAAGTTCGCGCCAATTGGACTTACAAACATGTTCAACTGTGGAGGCACACTCCAAGAGTTGGAGTTCAAAAGTACCGCGGTTGAGTTTAGTGCCATGATCAAAGTTAAGGGTGGCGGCATGTTCCTGGCCTACTCAAGTGAGTCACCAAAGAAATGTTGTTTGAATGGTGCTGAGCTAGCTTTTGAGTGGTCTGCTGATGGCAAATTGAACTTGAGTGTTCCTTGGGTTGAACAAGCTGCTGGCATTTCTGATGTGCTTTTTGCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

865

Amino Acids

96.0

Weight (kDa)

5.81

Isoelectric Point (pI)

32.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 848 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AasI GACNNNNNNGTC 2 cut(s) 331, 2242
Acc65I GGTACC 1 cut(s) 1916
AccB1I GGYRCC 2 cut(s) 4, 1916
AccB7I CCANNNNNTGG 1 cut(s) 2386
AccI GTMKAC 3 cut(s) 335, 1701, 2202
AccII CGCG 3 cut(s) 766, 2336, 2405
AclWI GGATC 8 cut(s) 11, 947, 1670, 1682, 1683, 1720, 1831, 2093
AcsI RAATTY 5 cut(s) 532, 668, 740, 1811, 2004
AcuI CTGAAG 8 cut(s) 141, 842, 1055, 1100, 1458, 1541, 2184, 2208
AfaI GTAC 4 cut(s) 1576, 1918, 2195, 2401
AfiI CCNNNNNNNGG 6 cut(s) 261, 453, 826, 1954, 2212, 2386
AflIII ACRYGT 3 cut(s) 1782, 2131, 2355
AhdI GACNNNNNGTC 1 cut(s) 2158
AjnI CCWGG 5 cut(s) 469, 1270, 1318, 1952, 2451
AjuI GAANNNNNNNTTGG 2 cut(s) 2107, 2139
AleI CACNNNNGTG 1 cut(s) 222
Alw21I GWGCWC 3 cut(s) 393, 683, 2226
Alw26I GTCTC 1 cut(s) 1318
Alw44I GTGCAC 1 cut(s) 679
AlwI GGATC 8 cut(s) 11, 947, 1670, 1682, 1683, 1720, 1831, 2093
AlwNI CAGNNNCTG 2 cut(s) 1078, 1959
AoxI GGCC 8 cut(s) 1023, 1179, 1268, 1507, 1756, 2092, 2317, 2454
ApaLI GTGCAC 1 cut(s) 679
ApeKI GCWGC 4 cut(s) 265, 1597, 2168, 2564
ApoI RAATTY 5 cut(s) 532, 668, 740, 1811, 2004
Asp700I GAANNNNTTC 3 cut(s) 1147, 1181, 1565
Asp718I GGTACC 1 cut(s) 1916
AspLEI GCGC 2 cut(s) 1736, 2338
AspS9I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
AsuC2I CCSGG 2 cut(s) 404, 1910
AsuHPI GGTGA 8 cut(s) 488, 1033, 1118, 1202, 1308, 1366, 1667, 2463
AsuII TTCGAA 1 cut(s) 1059
AsuNHI GCTAGC 2 cut(s) 1589, 2503
AvaII GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
BaeGI GKGCMC 1 cut(s) 683
BamHI GGATCC 1 cut(s) 1675
BanI GGYRCC 2 cut(s) 4, 1916
BanII GRGCYC 1 cut(s) 2226
BarI GAAGNNNNNNTAC 2 cut(s) 1889, 1921
BbsI GAAGAC 2 cut(s) 650, 1088
Bbv12I GWGCWC 3 cut(s) 393, 683, 2226
BbvCI CCTCAGC 1 cut(s) 2207
BbvI GCAGC 4 cut(s) 252, 1609, 2180, 2551
BccI CCATC 7 cut(s) 443, 621, 779, 1747, 1907, 2281, 2519
BceAI ACGGC 2 cut(s) 1122, 1494
BcgI CGANNNNNNTGC 2 cut(s) 2314, 2348
BciT130I CCWGG 5 cut(s) 471, 1272, 1320, 1954, 2453
BciVI GTATCC 2 cut(s) 823, 1501
BclI TGATCA 2 cut(s) 1393, 2424
BcnI CCSGG 2 cut(s) 404, 1910
BcoDI GTCTC 1 cut(s) 1318
BfaI CTAG 8 cut(s) 513, 659, 842, 870, 1325, 1590, 2315, 2504
BfmI CTRYAG 3 cut(s) 595, 705, 910
BfuI GTATCC 2 cut(s) 823, 1501
BisI GCNGC 5 cut(s) 266, 1598, 2169, 2443, 2565
BlpI GCTNAGC 1 cut(s) 2499
BlsI GCNGC 5 cut(s) 267, 1599, 2170, 2444, 2566
Bme1390I CCNGG 7 cut(s) 404, 471, 1272, 1320, 1910, 1954, 2453
Bme18I GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
BmeRI GACNNNNNGTC 1 cut(s) 2158
BmgT120I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
BmrFI CCNGG 7 cut(s) 404, 471, 1272, 1320, 1910, 1954, 2453
BmsI GCATC 5 cut(s) 318, 679, 807, 928, 2239
BmtI GCTAGC 2 cut(s) 1593, 2507
BpiI GAAGAC 2 cut(s) 650, 1088
BplI GAGNNNNNCTC 6 cut(s) 239, 271, 600, 632, 2361, 2393
BpmI CTGGAG 2 cut(s) 1056, 1473
Bpu10I CCTNAGC 2 cut(s) 305, 2207
Bpu1102I GCTNAGC 1 cut(s) 2499
Bpu14I TTCGAA 1 cut(s) 1059
BpuEI CTTGAG 3 cut(s) 1216, 2446, 2560
BpuMI CCSGG 2 cut(s) 404, 1910
BsaBI GATNNNNATC 1 cut(s) 1884
BsaWI WCCGGW 2 cut(s) 45, 1846
BsaXI ACNNNNNCTCC 6 cut(s) 398, 428, 1446, 1476, 1497, 1527
Bsc4I CCNNNNNNNGG 6 cut(s) 261, 453, 826, 1954, 2212, 2386
Bse118I RCCGGY 1 cut(s) 2302
Bse1I ACTGG 5 cut(s) 29, 447, 1456, 1582, 1709
Bse3DI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
Bse8I GATNNNNATC 1 cut(s) 1884
BseBI CCWGG 5 cut(s) 471, 1272, 1320, 1954, 2453
BseGI GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BseJI GATNNNNATC 1 cut(s) 1884
BseLI CCNNNNNNNGG 6 cut(s) 261, 453, 826, 1954, 2212, 2386
BseMI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
BseMII CTCAG 9 cut(s) 171, 270, 301, 996, 1300, 1878, 2112, 2221, 2490
BseNI ACTGG 5 cut(s) 29, 447, 1456, 1582, 1709
BseRI GAGGAG 1 cut(s) 902
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 4 cut(s) 252, 1609, 2180, 2551
BsgI GTGCAG 1 cut(s) 621
Bsh1236I CGCG 3 cut(s) 766, 2336, 2405
BshFI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BshNI GGYRCC 2 cut(s) 4, 1916
BsiHKAI GWGCWC 3 cut(s) 393, 683, 2226
BsiSI CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
BslI CCNNNNNNNGG 6 cut(s) 261, 453, 826, 1954, 2212, 2386
BsmAI GTCTC 1 cut(s) 1318
BsmI GAATGC 2 cut(s) 692, 1139
BsnI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
Bsp119I TTCGAA 1 cut(s) 1059
Bsp1286I GDGCHC 3 cut(s) 393, 683, 2226
Bsp1720I GCTNAGC 1 cut(s) 2499
BspANI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BspCNI CTCAG 9 cut(s) 170, 269, 300, 997, 1299, 1877, 2111, 2220, 2491
BspFNI CGCG 3 cut(s) 766, 2336, 2405
BspHI TCATGA 1 cut(s) 489
BspOI GCTAGC 2 cut(s) 1593, 2507
BspPI GGATC 8 cut(s) 11, 947, 1670, 1682, 1683, 1720, 1831, 2093
BspQI GCTCTTC 1 cut(s) 468
BspT104I TTCGAA 1 cut(s) 1059
BspT107I GGYRCC 2 cut(s) 4, 1916
BsrDI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
BsrFI RCCGGY 1 cut(s) 2302
BsrI ACTGG 5 cut(s) 29, 447, 1456, 1582, 1709
BssAI RCCGGY 1 cut(s) 2302
BssT1I CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
Bst2UI CCWGG 5 cut(s) 471, 1272, 1320, 1954, 2453
Bst4CI ACNGT 7 cut(s) 649, 706, 881, 972, 1312, 1996, 2366
Bst6I CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
BstBI TTCGAA 1 cut(s) 1059
BstC8I GCNNGC 9 cut(s) 163, 1002, 1239, 1521, 1591, 1595, 1934, 2505, 2569
BstDSI CCRYGG 3 cut(s) 764, 1138, 2403
BstENI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
BstF5I GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BstFNI CGCG 3 cut(s) 766, 2336, 2405
BstHHI GCGC 2 cut(s) 1736, 2338
BstMAI GTCTC 1 cut(s) 1318
BstMWI GCNNNNNNNGC 4 cut(s) 599, 1143, 1152, 2504
BstNI CCWGG 5 cut(s) 471, 1272, 1320, 1954, 2453
BstNSI RCATGY 8 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359, 2449
BstSCI CCNGG 7 cut(s) 402, 469, 1270, 1318, 1908, 1952, 2451
BstSFI CTRYAG 3 cut(s) 595, 705, 910
BstSLI GKGCMC 1 cut(s) 683
BstUI CGCG 3 cut(s) 766, 2336, 2405
BstV1I GCAGC 4 cut(s) 252, 1609, 2180, 2551
BstV2I GAAGAC 2 cut(s) 650, 1088
BstX2I RGATCY 2 cut(s) 1675, 1687
BstYI RGATCY 2 cut(s) 1675, 1687
BsuI GTATCC 2 cut(s) 823, 1501
BsuRI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BtgI CCRYGG 3 cut(s) 764, 1138, 2403
BtsCI GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BtsI GCAGTG 3 cut(s) 1126, 1162, 1729
BtsIMutI CAGTG 4 cut(s) 1126, 1162, 1449, 1729
Cac8I GCNNGC 9 cut(s) 163, 1002, 1239, 1521, 1591, 1595, 1934, 2505, 2569
CaiI CAGNNNCTG 2 cut(s) 1078, 1959
CciI TCATGA 1 cut(s) 489
CfoI GCGC 2 cut(s) 1736, 2338
Cfr10I RCCGGY 1 cut(s) 2302
Cfr13I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
Cfr42I CCGCGG 2 cut(s) 767, 2406
CpoI CGGWCCG 1 cut(s) 48
CseI GACGC 1 cut(s) 516
Csp6I GTAC 4 cut(s) 1575, 1917, 2194, 2400
CspI CGGWCCG 1 cut(s) 48
CviQI GTAC 4 cut(s) 1575, 1917, 2194, 2400
DraI TTTAAA 1 cut(s) 1210
DrdI GACNNNNNNGTC 2 cut(s) 331, 2242
DriI GACNNNNNGTC 1 cut(s) 2158
DseDI GACNNNNNNGTC 2 cut(s) 331, 2242
Eam1104I CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
Eam1105I GACNNNNNGTC 1 cut(s) 2158
EarI CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
EciI GGCGGA 1 cut(s) 2216
Ecl136II GAGCTC 1 cut(s) 2224
Eco130I CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
Eco147I AGGCCT 1 cut(s) 1181
Eco24I GRGCYC 1 cut(s) 2226
Eco32I GATATC 1 cut(s) 1099
Eco47I GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
Eco53kI GAGCTC 1 cut(s) 2224
Eco57I CTGAAG 8 cut(s) 141, 842, 1055, 1100, 1458, 1541, 2184, 2208
EcoICRI GAGCTC 1 cut(s) 2224
EcoNI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
EcoO109I RGGNCCY 4 cut(s) 817, 932, 1024, 2068
EcoRII CCWGG 5 cut(s) 469, 1270, 1318, 1952, 2451
EcoRV GATATC 1 cut(s) 1099
EcoT14I CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 2226
ErhI CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
FauI CCCGC 2 cut(s) 771, 1364
FauNDI CATATG 2 cut(s) 418, 1722
FbaI TGATCA 2 cut(s) 1393, 2424
FblI GTMKAC 3 cut(s) 335, 1701, 2202
Fnu4HI GCNGC 5 cut(s) 266, 1598, 2169, 2443, 2565
FokI GGATG 9 cut(s) 453, 701, 821, 1232, 1374, 1478, 1765, 1925, 2252
FriOI GRGCYC 1 cut(s) 2226
Fsp4HI GCNGC 5 cut(s) 266, 1598, 2169, 2443, 2565
FspBI CTAG 8 cut(s) 513, 659, 842, 870, 1325, 1590, 2315, 2504
GlaI GCGC 2 cut(s) 1735, 2337
GluI GCNGC 5 cut(s) 266, 1598, 2169, 2443, 2565
GsuI CTGGAG 2 cut(s) 1056, 1473
HaeIII GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
HapII CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
HgaI GACGC 1 cut(s) 516
HhaI GCGC 2 cut(s) 1736, 2338
Hin6I GCGC 2 cut(s) 1734, 2336
HinP1I GCGC 2 cut(s) 1734, 2336
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 2 cut(s) 89, 1898
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
HphI GGTGA 8 cut(s) 488, 1033, 1118, 1202, 1308, 1366, 1667, 2463
Hpy166II GTNNAC 6 cut(s) 336, 664, 681, 1702, 1906, 2203
Hpy8I GTNNAC 6 cut(s) 336, 664, 681, 1702, 1906, 2203
Hpy99I CGWCG 1 cut(s) 532
HpyAV CCTTC 8 cut(s) 743, 817, 954, 1003, 1037, 1103, 1171, 1192
HpyCH4III ACNGT 7 cut(s) 649, 706, 881, 972, 1312, 1996, 2366
HpyCH4IV ACGT 1 cut(s) 2196
HpyF10VI GCNNNNNNNGC 4 cut(s) 599, 1143, 1152, 2504
HpySE526I ACGT 1 cut(s) 2196
HspAI GCGC 2 cut(s) 1734, 2336
KflI GGGWCCC 1 cut(s) 2068
KpnI GGTACC 1 cut(s) 1920
Ksp22I TGATCA 2 cut(s) 1393, 2424
KspAI GTTAAC 1 cut(s) 664
KspI CCGCGG 2 cut(s) 767, 2406
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 5 cut(s) 198, 946, 1516, 2039, 2229
Lsp1109I GCAGC 4 cut(s) 252, 1609, 2180, 2551
LweI GCATC 5 cut(s) 318, 679, 807, 928, 2239
MaeI CTAG 8 cut(s) 513, 659, 842, 870, 1325, 1590, 2315, 2504
MaeII ACGT 1 cut(s) 2196
MfeI CAATTG 3 cut(s) 987, 2047, 2340
MflI RGATCY 2 cut(s) 1675, 1687
MhlI GDGCHC 3 cut(s) 393, 683, 2226
MmeI TCCRAC 2 cut(s) 220, 2367
Mph1103I ATGCAT 1 cut(s) 694
MroXI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
MseI TTAA 7 cut(s) 663, 701, 1209, 1893, 2016, 2328, 2433
MslI CAYNNNNRTG 5 cut(s) 222, 803, 1751, 1803, 2136
MspA1I CMGCKG 2 cut(s) 766, 2405
MspI CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
MspR9I CCNGG 7 cut(s) 404, 471, 1272, 1320, 1910, 1954, 2453
MunI CAATTG 3 cut(s) 987, 2047, 2340
Mva1269I GAATGC 2 cut(s) 692, 1139
MvaI CCWGG 5 cut(s) 471, 1272, 1320, 1954, 2453
MvnI CGCG 3 cut(s) 766, 2336, 2405
MwoI GCNNNNNNNGC 4 cut(s) 599, 1143, 1152, 2504
NciI CCSGG 2 cut(s) 404, 1910
NdeI CATATG 2 cut(s) 418, 1722
NheI GCTAGC 2 cut(s) 1589, 2503
NmeAIII GCCGAG 1 cut(s) 526
NmuCI GTSAC 7 cut(s) 146, 1039, 1157, 1190, 1655, 1859, 2469
NsiI ATGCAT 1 cut(s) 694
NspI RCATGY 8 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359, 2449
NspV TTCGAA 1 cut(s) 1059
OliI CACNNNNGTG 1 cut(s) 222
PaeI GCATGC 3 cut(s) 1004, 1241, 1597
PagI TCATGA 1 cut(s) 489
PceI AGGCCT 1 cut(s) 1181
PciI ACATGT 3 cut(s) 1782, 2131, 2355
PciSI GCTCTTC 1 cut(s) 468
PctI GAATGC 2 cut(s) 692, 1139
PdmI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
PfeI GAWTC 2 cut(s) 106, 237
PflFI GACNNNGTC 1 cut(s) 485
PflMI CCANNNNNTGG 1 cut(s) 2386
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 5 cut(s) 267, 1599, 2170, 2444, 2566
PpuMI RGGWCCY 3 cut(s) 817, 932, 2068
PscI ACATGT 3 cut(s) 1782, 2131, 2355
PsiI TTATAA 1 cut(s) 425
Psp124BI GAGCTC 1 cut(s) 2226
Psp5II RGGWCCY 3 cut(s) 817, 932, 2068
Psp6I CCWGG 5 cut(s) 469, 1270, 1318, 1952, 2451
PspGI CCWGG 5 cut(s) 469, 1270, 1318, 1952, 2451
PspPI GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
PspPPI RGGWCCY 3 cut(s) 817, 932, 2068
PstNI CAGNNNCTG 2 cut(s) 1078, 1959
PsuI RGATCY 2 cut(s) 1675, 1687
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 4 cut(s) 1576, 1918, 2195, 2401
RsaNI GTAC 4 cut(s) 1575, 1917, 2194, 2400
RseI CAYNNNNRTG 5 cut(s) 222, 803, 1751, 1803, 2136
Rsr2I CGGWCCG 1 cut(s) 48
RsrII CGGWCCG 1 cut(s) 48
SacI GAGCTC 1 cut(s) 2226
SacII CCGCGG 2 cut(s) 767, 2406
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 7 cut(s) 663, 701, 1209, 1893, 2016, 2328, 2433
SatI GCNGC 5 cut(s) 266, 1598, 2169, 2443, 2565
Sau96I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
ScrFI CCNGG 7 cut(s) 404, 471, 1272, 1320, 1910, 1954, 2453
SduI GDGCHC 3 cut(s) 393, 683, 2226
SfaNI GCATC 5 cut(s) 318, 679, 807, 928, 2239
SfcI CTRYAG 3 cut(s) 595, 705, 910
Sfr303I CCGCGG 2 cut(s) 767, 2406
SfuI TTCGAA 1 cut(s) 1059
SgrBI CCGCGG 2 cut(s) 767, 2406
SinI GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
SmiMI CAYNNNNRTG 5 cut(s) 222, 803, 1751, 1803, 2136
SmlI CTYRAG 3 cut(s) 1195, 2461, 2539
SmoI CTYRAG 3 cut(s) 1195, 2461, 2539
SphI GCATGC 3 cut(s) 1004, 1241, 1597
SseBI AGGCCT 1 cut(s) 1181
SspMI CTAG 8 cut(s) 513, 659, 842, 870, 1325, 1590, 2315, 2504
SstI GAGCTC 1 cut(s) 2226
StuI AGGCCT 1 cut(s) 1181
StyD4I CCNGG 7 cut(s) 402, 469, 1270, 1318, 1908, 1952, 2451
StyI CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
TaaI ACNGT 7 cut(s) 649, 706, 881, 972, 1312, 1996, 2366
TaiI ACGT 1 cut(s) 2199
TaqI TCGA 3 cut(s) 530, 974, 1059
TauI GCSGC 1 cut(s) 2445
TfiI GAWTC 2 cut(s) 106, 237
Tru1I TTAA 7 cut(s) 663, 701, 1209, 1893, 2016, 2328, 2433
Tru9I TTAA 7 cut(s) 663, 701, 1209, 1893, 2016, 2328, 2433
TscAI CASTG 4 cut(s) 1126, 1162, 1456, 1736
TseFI GTSAC 7 cut(s) 146, 1039, 1157, 1190, 1655, 1859, 2469
TseI GCWGC 4 cut(s) 265, 1597, 2168, 2564
Tsp45I GTSAC 7 cut(s) 146, 1039, 1157, 1190, 1655, 1859, 2469
TspRI CASTG 4 cut(s) 1126, 1162, 1456, 1736
Tth111I GACNNNGTC 1 cut(s) 485
Van91I CCANNNNNTGG 1 cut(s) 2386
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
XagI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
XapI RAATTY 5 cut(s) 532, 668, 740, 1811, 2004
XceI RCATGY 8 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359, 2449
XmiI GTMKAC 3 cut(s) 335, 1701, 2202
XmnI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
XspI CTAG 8 cut(s) 513, 659, 842, 870, 1325, 1590, 2315, 2504
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.