Rh1DG246900

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002202
Physical Location & Seq
Reverse (-)
46131152 .. 46133604
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG246900.1

Sequence Viewer

Length: 2133 bp
ATGATCTTTGCTGAGAGTGGTTCTACCCAAGTGAAAGCATCGAAGTTCGATGCCATCGCTTATGTTCATGTGTCCGACAATCCCTACACCTTGATGAAAGAGGCCTACAGTGCTCTGAGGGTCCATCTCAATACCTTTAGGCTCCTTGAAGAAAAATCGGCACCAAATCTAGTTGATAAATTTGGTTGGTGCACTTGGGATGCCTTCTACTTAACTGTGGAACCTGTTGGTGTCTCCCATGGTGTAAATGAATTCGTTGAAGCCGGTGTACCCCCAAGGTTTCTCATCATTGATGATGGTTGGCAAAGCATTAATTTCGATGATGATGAGAACCCGAATGAGGATGCGAAGAATCTAGTCATTGGTGGGACTCAAATGACTGCTAGGCTTCACAGGCTTGAGGAGTGTGACAAGTTCAAAAACTACAAAGGTGGATCTTTACTGGGTCCTCATGCACCTTCATTTGATCCAAAAAGGCCAAAGATGTTGATATCCAAGGCAATTGAATTAGAGAATGTTGAAAAGGCTCGTGATAAGGCCATTCAATCTGGAGTCACTGATTTGTCCGAGTTCCAAAAACAAATGCAGAAGCATAAACAAGAGTTGGATATGATAATTGGTGGTGGAGAAGAAGGCAGCACTACTAGTAGTGTTTTGAAAGAAGGAAGCTGTAGAAGCTGTTCTTGCAAGGCTAAAAATTATGGAATGAAGTCTTTCACATGCGACTTGAGAACCAAGTTCAAGAATTTGGATGATATTTATGTATGGCATGCTCTTTGTGGTGCCTGGGGTGGTGTTAAACCAGGTACAACTCATCTCAATTCAAAGATCATCCCCTGCAAAGTCTCTCCAGGACTAGATGGTTCCATGTCTGACCTAGCTGTGGTGAAACTAGTTGAGGGTGGGATTGGACTCGTCCATCCTGATCAAGCTGGTGATTTCTATAATTCTATGCACTCTTACCTTTCTGAAGCTGGTATCACAGGAGTCAAAGTGGATGTTATTCATACTCTTGAATATGTATCTGAGGAATTCGGAGGCGGTGTTGAGCTTGCAAAGGCTTATTACAAGGGGCTGACTTCTTCTCTTGTAAAGAACTTCAATGGAACTGGACTTATTGCTAGCATGCAACAATGCAATGATTTCTTCTTCCTTGGAACAAAACAAATCTCTATTGGAAGAGTTGGTGATGATTTTTGGTTTCAGGATCCAAATGGAGACCCCATGGGAGTTTACTGGCTACAAGGTGTCCATATGATCCATTGTGCCTACAACAGCATGTGGATGGGTCAATTCATTCAGCCTGATTGGGATATGTTCCAGTCAGATCATCTGTGTGCCAAATTTCATGCCGGATCTAGGGCTATCTGTGGAGGTCCGGTGTATATAAGTGACTCGGTTGGTGGTCACAATTTTGATCTTATTAGGCAGCTTGTTTACCCAGATGGTACCATCCCCAAGTGCCAGCACTTTGCTCTTCCAACTAGGGACTGCCTATTCAAGAACCCCTTATTTGACAGCAAGACTGCTCTCAAAATTTGGAATGTCAACAAGTTTGGTGGTGTGATGGGAGCTTTCAACTGCCAAGGGGCTTGTTGGGACCCAAAAGAAAAAAGAATCAAAGGCTACTCCCAGTGCTACAAGGAAATCCTCTATTCAGTGCATGTTTCTGAAATTGAATGGGACCAAAAGATGGAAGCCGCCCATTTGGGTAAGGCCGAAGAATATGTGGTCTATCTCAATCAGGCCAATGAACTCTTCCAAATGACCCCCAAATCCGATCCCATTCAAATGGTGATCCAACCATCTTCGTTCGAGATTTTCAGCTTCGTGCCAGTACAAAAGCTCGGCTCCAACAACACCAAGTTTGCGCCGATTGGACTGACAAACATGTTCAACAGTGGAGGCACTATCCAAGAGCTGGATTATACAAGTGGCATGGAATCTTATAGTGCAAAGATCAAGGTTAAAGGTGGAGGCAACTTCTTGGCCTACTCAAGTCAGTCTCCGAAGAAGTGTTGTTTGAATGGTGCTGTAGTGACTTTTGAGTGGTCTGCTTCTGGTAAACTGTCCCTGAATCTTCCTTGGGTTGAAGAAGCGGCTGGAATTTCTGATGTCGTTCTTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

710

Amino Acids

78.6

Weight (kDa)

5.85

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 2 - 693 1.9e-273 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1448
AccB1I GGYRCC 3 cut(s) 160, 782, 1448
AccB7I CCANNNNNTGG 2 cut(s) 1693, 1921
AciI CCGC 3 cut(s) 1041, 1701, 2099
AclWI GGATC 8 cut(s) 442, 461, 1202, 1215, 1252, 1363, 1775, 1792
AcsI RAATTY 7 cut(s) 179, 251, 745, 1031, 1343, 1536, 2106
AcuI CTGAAG 1 cut(s) 990
AfaI GTAC 4 cut(s) 270, 808, 1450, 1839
AfiI CCNNNNNNNGG 5 cut(s) 340, 883, 1359, 1693, 1921
AflIII ACRYGT 1 cut(s) 1890
AhlI ACTAGT 2 cut(s) 644, 892
AjnI CCWGG 3 cut(s) 785, 802, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 1742, 1774
Alw21I GWGCWC 2 cut(s) 115, 194
Alw26I GTCTC 4 cut(s) 238, 850, 1212, 2010
Alw44I GTGCAC 1 cut(s) 190
AlwI GGATC 8 cut(s) 442, 461, 1202, 1215, 1252, 1363, 1775, 1792
AoxI GGCC 6 cut(s) 102, 476, 537, 1716, 1746, 1989
ApaLI GTGCAC 1 cut(s) 190
ApeKI GCWGC 2 cut(s) 636, 1429
ApoI RAATTY 7 cut(s) 179, 251, 745, 1031, 1343, 1536, 2106
AseI ATTAAT 1 cut(s) 312
Asp700I GAANNNNTTC 2 cut(s) 679, 713
Asp718I GGTACC 1 cut(s) 1448
AspLEI GCGC 1 cut(s) 1873
AspS9I GGNCC 5 cut(s) 121, 446, 1376, 1600, 1684
AsuHPI GGTGA 4 cut(s) 898, 947, 1199, 1807
AsuNHI GCTAGC 1 cut(s) 1121
AvaII GGWCC 5 cut(s) 121, 446, 1376, 1600, 1684
BaeGI GKGCMC 1 cut(s) 194
BamHI GGATCC 1 cut(s) 1207
BanI GGYRCC 3 cut(s) 160, 782, 1448
BarI GAAGNNNNNNTAC 2 cut(s) 252, 284
BauI CACGAG 1 cut(s) 528
Bbv12I GWGCWC 2 cut(s) 115, 194
BbvI GCAGC 2 cut(s) 648, 1441
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BciT130I CCWGG 3 cut(s) 787, 804, 852
BclI TGATCA 1 cut(s) 925
BcoDI GTCTC 4 cut(s) 238, 850, 1212, 2010
BcuI ACTAGT 2 cut(s) 644, 892
BfmI CTRYAG 3 cut(s) 106, 670, 2034
BisI GCNGC 4 cut(s) 637, 1430, 1701, 2100
BlsI GCNGC 4 cut(s) 638, 1431, 1702, 2101
Bme1390I CCNGG 3 cut(s) 787, 804, 852
Bme18I GGWCC 5 cut(s) 121, 446, 1376, 1600, 1684
BmgT120I GGNCC 5 cut(s) 121, 446, 1376, 1600, 1684
BmrFI CCNGG 3 cut(s) 787, 804, 852
BmrI ACTGGG 2 cut(s) 452, 1627
BmsI GCATC 4 cut(s) 40, 47, 190, 334
BmtI GCTAGC 1 cut(s) 1125
BmuI ACTGGG 2 cut(s) 452, 1627
BpmI CTGGAG 2 cut(s) 570, 834
BpuEI CTTGAG 3 cut(s) 419, 748, 1981
BsaI GGTCTC 1 cut(s) 1212
BsaJI CCNNGG 8 cut(s) 238, 275, 495, 786, 1153, 1224, 1585, 2084
BsaWI WCCGGW 1 cut(s) 1378
BsaXI ACNNNNNCTCC 6 cut(s) 978, 1008, 1029, 1059, 1365, 1395
Bsc4I CCNNNNNNNGG 5 cut(s) 340, 883, 1359, 1693, 1921
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 6 cut(s) 447, 1114, 1241, 1321, 1633, 1835
Bse3DI GCAATG 1 cut(s) 1144
BseBI CCWGG 3 cut(s) 787, 804, 852
BseDI CCNNGG 8 cut(s) 238, 275, 495, 786, 1153, 1224, 1585, 2084
BseGI GGATG 8 cut(s) 205, 349, 757, 831, 919, 1003, 1290, 1452
BseLI CCNNNNNNNGG 5 cut(s) 340, 883, 1359, 1693, 1921
BseMI GCAATG 1 cut(s) 1144
BseMII CTCAG 2 cut(s) 107, 1017
BseNI ACTGG 6 cut(s) 447, 1114, 1241, 1321, 1633, 1835
BseRI GAGGAG 1 cut(s) 416
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 2 cut(s) 648, 1441
BshFI GGCC 6 cut(s) 104, 478, 539, 1718, 1748, 1991
BshNI GGYRCC 3 cut(s) 160, 782, 1448
BsiHKAI GWGCWC 2 cut(s) 115, 194
BsiSI CCGG 3 cut(s) 264, 1353, 1379
BslFI GGGAC 5 cut(s) 382, 1502, 1613, 1697, 2056
BslI CCNNNNNNNGG 5 cut(s) 340, 883, 1359, 1693, 1921
BsmAI GTCTC 4 cut(s) 238, 850, 1212, 2010
BsmFI GGGAC 5 cut(s) 382, 1502, 1613, 1697, 2056
BsnI GGCC 6 cut(s) 104, 478, 539, 1718, 1748, 1991
Bso31I GGTCTC 1 cut(s) 1212
Bsp1286I GDGCHC 2 cut(s) 115, 194
Bsp19I CCATGG 2 cut(s) 238, 1224
BspACI CCGC 3 cut(s) 1041, 1701, 2099
BspANI GGCC 6 cut(s) 104, 478, 539, 1718, 1748, 1991
BspCNI CTCAG 3 cut(s) 4, 108, 1018
BspOI GCTAGC 1 cut(s) 1125
BspPI GGATC 8 cut(s) 442, 461, 1202, 1215, 1252, 1363, 1775, 1792
BspQI GCTCTTC 1 cut(s) 1482
BspT107I GGYRCC 3 cut(s) 160, 782, 1448
BspTNI GGTCTC 1 cut(s) 1212
BsrDI GCAATG 1 cut(s) 1144
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 6 cut(s) 447, 1114, 1241, 1321, 1633, 1835
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 8 cut(s) 238, 275, 495, 786, 1153, 1224, 1585, 2084
BssSI CACGAG 1 cut(s) 528
BssT1I CCWWGG 7 cut(s) 238, 275, 495, 1153, 1224, 1585, 2084
Bst2BI CACGAG 1 cut(s) 528
Bst2UI CCWGG 3 cut(s) 787, 804, 852
Bst4CI ACNGT 4 cut(s) 110, 217, 1901, 2070
Bst6I CTCTTC 3 cut(s) 1174, 1482, 1763
BstC8I GCNNGC 5 cut(s) 771, 1053, 1123, 1127, 1466
BstDEI CTNAG 3 cut(s) 12, 116, 1026
BstDSI CCRYGG 2 cut(s) 238, 1224
BstF5I GGATG 8 cut(s) 205, 349, 757, 831, 919, 1003, 1290, 1452
BstHHI GCGC 1 cut(s) 1873
BstMAI GTCTC 4 cut(s) 238, 850, 1212, 2010
BstMWI GCNNNNNNNGC 4 cut(s) 110, 394, 675, 684
BstNI CCWGG 3 cut(s) 787, 804, 852
BstNSI RCATGY 6 cut(s) 723, 773, 1129, 1282, 1667, 1894
BstSCI CCNGG 3 cut(s) 785, 802, 850
BstSFI CTRYAG 3 cut(s) 106, 670, 2034
BstSLI GKGCMC 1 cut(s) 194
BstV1I GCAGC 2 cut(s) 648, 1441
BstX2I RGATCY 3 cut(s) 434, 1207, 1355
BstXI CCANNNNNNTGG 1 cut(s) 1792
BstYI RGATCY 3 cut(s) 434, 1207, 1355
BsuRI GGCC 6 cut(s) 104, 478, 539, 1718, 1748, 1991
BtgI CCRYGG 2 cut(s) 238, 1224
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 8 cut(s) 205, 349, 757, 831, 919, 1003, 1290, 1452
BtsIMutI CAGTG 5 cut(s) 115, 555, 1640, 1665, 1906
Cac8I GCNNGC 5 cut(s) 771, 1053, 1123, 1127, 1466
CfoI GCGC 1 cut(s) 1873
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 5 cut(s) 121, 446, 1376, 1600, 1684
CsiI ACCWGGT 1 cut(s) 802
Csp6I GTAC 4 cut(s) 269, 807, 1449, 1838
CspCI CAANNNNNGTGG 2 cut(s) 1540, 1575
CviQI GTAC 4 cut(s) 269, 807, 1449, 1838
DdeI CTNAG 3 cut(s) 12, 116, 1026
Eam1104I CTCTTC 3 cut(s) 1174, 1482, 1763
EarI CTCTTC 3 cut(s) 1174, 1482, 1763
Eco130I CCWWGG 7 cut(s) 238, 275, 495, 1153, 1224, 1585, 2084
Eco147I AGGCCT 1 cut(s) 104
Eco31I GGTCTC 1 cut(s) 1212
Eco32I GATATC 1 cut(s) 492
Eco47I GGWCC 5 cut(s) 121, 446, 1376, 1600, 1684
Eco57I CTGAAG 1 cut(s) 990
EcoO109I RGGNCCY 2 cut(s) 446, 1600
EcoRI GAATTC 2 cut(s) 251, 1031
EcoRII CCWGG 3 cut(s) 785, 802, 850
EcoRV GATATC 1 cut(s) 492
EcoT14I CCWWGG 7 cut(s) 238, 275, 495, 1153, 1224, 1585, 2084
ErhI CCWWGG 7 cut(s) 238, 275, 495, 1153, 1224, 1585, 2084
FalI AAGNNNNNCTT 4 cut(s) 667, 699, 1493, 1525
FaqI GGGAC 5 cut(s) 382, 1502, 1613, 1697, 2056
FauNDI CATATG 1 cut(s) 1254
FbaI TGATCA 1 cut(s) 925
Fnu4HI GCNGC 4 cut(s) 637, 1430, 1701, 2100
FokI GGATG 8 cut(s) 212, 356, 764, 818, 906, 1010, 1297, 1439
Fsp4HI GCNGC 4 cut(s) 637, 1430, 1701, 2100
GlaI GCGC 1 cut(s) 1872
GluI GCNGC 4 cut(s) 637, 1430, 1701, 2100
GsuI CTGGAG 2 cut(s) 570, 834
HaeIII GGCC 6 cut(s) 104, 478, 539, 1718, 1748, 1991
HapII CCGG 3 cut(s) 264, 1353, 1379
HhaI GCGC 1 cut(s) 1873
Hin6I GCGC 1 cut(s) 1871
HinP1I GCGC 1 cut(s) 1871
HincII GTYRAC 1 cut(s) 1549
HindII GTYRAC 1 cut(s) 1549
HinfI GANTC 9 cut(s) 352, 370, 552, 912, 987, 1394, 1617, 1943, 2077
HpaII CCGG 3 cut(s) 264, 1353, 1379
HphI GGTGA 4 cut(s) 898, 947, 1199, 1807
Hpy166II GTNNAC 6 cut(s) 192, 269, 1234, 1438, 1549, 2066
Hpy188III TCNNGA 8 cut(s) 530, 549, 742, 923, 1013, 1205, 1501, 1816
Hpy8I GTNNAC 6 cut(s) 192, 269, 1234, 1438, 1549, 2066
HpyAV CCTTC 4 cut(s) 214, 468, 626, 656
HpyCH4III ACNGT 4 cut(s) 110, 217, 1901, 2070
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 394, 675, 684
HpyF3I CTNAG 3 cut(s) 12, 116, 1026
HspAI GCGC 1 cut(s) 1871
KflI GGGWCCC 1 cut(s) 1600
KpnI GGTACC 1 cut(s) 1452
Ksp22I TGATCA 1 cut(s) 925
LguI GCTCTTC 1 cut(s) 1482
LmnI GCTCC 3 cut(s) 147, 1571, 1856
Lsp1109I GCAGC 2 cut(s) 648, 1441
LweI GCATC 4 cut(s) 40, 47, 190, 334
MabI ACCWGGT 1 cut(s) 802
MaeIII GTNAC 5 cut(s) 407, 553, 1391, 1406, 2038
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 3 cut(s) 434, 1207, 1355
MhlI GDGCHC 2 cut(s) 115, 194
MlyI GAGTC 5 cut(s) 364, 561, 906, 996, 1388
MmeI TCCRAC 5 cut(s) 99, 585, 1505, 1825, 1878
MroXI GAANNNNTTC 2 cut(s) 679, 713
MseI TTAA 4 cut(s) 212, 312, 798, 1968
MslI CAYNNNNRTG 4 cut(s) 92, 1283, 1335, 1790
MspI CCGG 3 cut(s) 264, 1353, 1379
MspR9I CCNGG 3 cut(s) 787, 804, 852
MunI CAATTG 1 cut(s) 501
MvaI CCWGG 3 cut(s) 787, 804, 852
MwoI GCNNNNNNNGC 4 cut(s) 110, 394, 675, 684
NcoI CCATGG 2 cut(s) 238, 1224
NdeI CATATG 1 cut(s) 1254
NheI GCTAGC 1 cut(s) 1121
NmeAIII GCCGAG 1 cut(s) 1827
NmuCI GTSAC 5 cut(s) 407, 553, 1391, 1406, 2038
NspI RCATGY 6 cut(s) 723, 773, 1129, 1282, 1667, 1894
PaeI GCATGC 2 cut(s) 773, 1129
PceI AGGCCT 1 cut(s) 104
PciI ACATGT 1 cut(s) 1890
PciSI GCTCTTC 1 cut(s) 1482
PdmI GAANNNNTTC 2 cut(s) 679, 713
PfeI GAWTC 4 cut(s) 352, 1617, 1943, 2077
PflMI CCANNNNNTGG 2 cut(s) 1693, 1921
PfoI TCCNGGA 1 cut(s) 850
PkrI GCNGC 4 cut(s) 638, 1431, 1702, 2101
PleI GAGTC 5 cut(s) 364, 560, 906, 995, 1388
PpsI GAGTC 5 cut(s) 364, 560, 906, 995, 1388
PpuMI RGGWCCY 2 cut(s) 446, 1600
PscI ACATGT 1 cut(s) 1890
PshBI ATTAAT 1 cut(s) 312
Psp5II RGGWCCY 2 cut(s) 446, 1600
Psp6I CCWGG 3 cut(s) 785, 802, 850
PspGI CCWGG 3 cut(s) 785, 802, 850
PspPI GGNCC 5 cut(s) 121, 446, 1376, 1600, 1684
PspPPI RGGWCCY 2 cut(s) 446, 1600
PsrI GAACNNNNNNTAC 2 cut(s) 664, 696
PsuI RGATCY 3 cut(s) 434, 1207, 1355
RsaI GTAC 4 cut(s) 270, 808, 1450, 1839
RsaNI GTAC 4 cut(s) 269, 807, 1449, 1838
RseI CAYNNNNRTG 4 cut(s) 92, 1283, 1335, 1790
SapI GCTCTTC 1 cut(s) 1482
SaqAI TTAA 4 cut(s) 212, 312, 798, 1968
SatI GCNGC 4 cut(s) 637, 1430, 1701, 2100
Sau96I GGNCC 5 cut(s) 121, 446, 1376, 1600, 1684
SchI GAGTC 5 cut(s) 364, 561, 906, 996, 1388
ScrFI CCNGG 3 cut(s) 787, 804, 852
SduI GDGCHC 2 cut(s) 115, 194
SexAI ACCWGGT 1 cut(s) 802
SfaNI GCATC 4 cut(s) 40, 47, 190, 334
SfcI CTRYAG 3 cut(s) 106, 670, 2034
SinI GGWCC 5 cut(s) 121, 446, 1376, 1600, 1684
SmiMI CAYNNNNRTG 4 cut(s) 92, 1283, 1335, 1790
SmlI CTYRAG 3 cut(s) 398, 727, 1996
SmoI CTYRAG 3 cut(s) 398, 727, 1996
SpeI ACTAGT 2 cut(s) 644, 892
SphI GCATGC 2 cut(s) 773, 1129
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 3 cut(s) 1041, 1701, 2099
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 3 cut(s) 785, 802, 850
StyI CCWWGG 7 cut(s) 238, 275, 495, 1153, 1224, 1585, 2084
TaaI ACNGT 4 cut(s) 110, 217, 1901, 2070
TaqI TCGA 4 cut(s) 41, 48, 318, 1815
TatI WGTACW 1 cut(s) 1837
TauI GCSGC 2 cut(s) 1703, 2102
TfiI GAWTC 4 cut(s) 352, 1617, 1943, 2077
Tru1I TTAA 4 cut(s) 212, 312, 798, 1968
Tru9I TTAA 4 cut(s) 212, 312, 798, 1968
TscAI CASTG 5 cut(s) 115, 562, 1640, 1665, 1906
TseFI GTSAC 5 cut(s) 407, 553, 1391, 1406, 2038
TseI GCWGC 2 cut(s) 636, 1429
Tsp45I GTSAC 5 cut(s) 407, 553, 1391, 1406, 2038
TspDTI ATGAA 9 cut(s) 56, 110, 264, 450, 722, 995, 1285, 1337, 1767
TspRI CASTG 5 cut(s) 115, 562, 1640, 1665, 1906
Van91I CCANNNNNTGG 2 cut(s) 1693, 1921
VneI GTGCAC 1 cut(s) 190
VpaK11BI GGWCC 5 cut(s) 121, 446, 1376, 1600, 1684
VspI ATTAAT 1 cut(s) 312
XapI RAATTY 7 cut(s) 179, 251, 745, 1031, 1343, 1536, 2106
XceI RCATGY 6 cut(s) 723, 773, 1129, 1282, 1667, 1894
XmnI GAANNNNTTC 2 cut(s) 679, 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.