Rw2G004970

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002202
Physical Location & Seq
Forward (+)
4676390 .. 4679276
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G004970.1

Sequence Viewer

Length: 2553 bp
ATGGCACCCCCTAATGAACCTGTTAATCCAATTCGAAATTTGTTCCATTGTGCGAGTTCGGAACTCTACTTCGACTTGTCTGATGGAAAGTTGAGTGTGAAAGGTGTTCCATTGCTCTCTGAAGTTCCAACCAATGTCACTCTCAGTCCCTTTCATTCAATCTGCCAACCTTCTTGTTCTGATGCAGGGTTTCTTGGGTTCAATAAGGAGGAACCTTCTGATAAGTTGACCAACTCCTTGGGTAGGTTCAGTAACAGGGATTTCCTTAGCATCTTTAGGTTCAAACCATGGTGGTCTACCATGTGGGTTGGGAAGTCTGGGTCGGATTTGCAGGTGGAGACACAATGGGTGCTCTTAGATATCCCTGAAATCAAGTCCTATGTCATAATCATACCGATCATTGAAGACAAATTCAGGTCTGCTCTTCTACCTGGTCCTGATGGACGTGTCATTGTCTTTGCTGAGAGTGGTTGTACCAAAGTGAAAACTTCATATTTTGATGCCATCGCTTATGTTCATGCTTCTGATAATCCCTACACCTTGATGAAAGAGGCCTATAGTGCTCTTAGGGTTCATCTCAATACCTTTAGGCTCCTAGAAGAGAAAACAGTCCCAAGTTTAGTTGACAAGTTTGGTTGGTGCACTTGGGATGCCTTCTATTTAACTGTAGAACCTGTTGGTGTTTTCCATGGGGTCAAGGAATTTGTTGAAGCCGGTGTTCCCCCAAGGTTTATCATCATTGATGATGGCTGGCAAAGTATCAATTTTGACAGTGACGAAAACCCGAATGAGGACGCACATCTAATCCTTGGTGGGACTCAAATGACTGGTAGGCTTCACAGGTTTGAGGAGTGTGACAAGTTTAGGAACTACAAAGGAGGATCTTTGTTGGATCCTAACGCTCCTTCATATGATTCCAACAGGCAGAAGAAGTTGATAGCCAAGGCACTTGAAGTTGAGGATATTGAGAAGGCTCGTGACAGGGCCACTCAATCAGGAGTCACTGATTTGTCCGAGTTCAAAACACAATGGCAGAAGCCTAAGCAAGAGTTGGATGTGATGATTGCTGAAGAAGAAAAAATTAGTGGTTCTGCTTCTACCAATGGCTGTGGAAGCTATGGTGGAATGAAGGCTTTCACAAGCGACTTGAGAACCAAATATAAGGGTTTGGATGATATTTATGTATGGCATGCTCTTTGTGGTGCCTGGGGTGGTGTAAAGCCCGGCGCAACCCATCTCAATGCAAAGGTCACTGCTGTTAATGTCTCTTCTGGACTCGACGGGTCGATGACTGATCTTGCTGTGGTGAAACTCATAGAGGGTGGCATTGGACTTGTTCATCCTGATCAAGCTGACGATTTCTTCAATTCTATGCACTCCTATCTTTCTGAAGTTGGTATAACAGGAGTCAAAGTGGACGTTATTCATAGTCTTGAGTTTTTATGTGAGGAACACGGAGGCCGTCTGGAGCTTCCAAAGGCATATTACAAGGGGCTTACTTCTTCCCTTGTAAATAACTTCAATGGGACTGGACTTATTTCTAGCATGCAACAATGCAATGACTTCTTCTTCCTTGGGACAAAGCAAATCTCTATTGGAAGAGTTGGTGATGACTTTTGGTTCCGTGGTGGAGATGGAGACCCCATGGCAGTTTATTGGTTACAAGGAGTTCATATGATCCATTGTGCTTACAACAGCATGTGGATGAGTCAGTTCATCCAACCTGATTGGGATATGTTCCAATCAGAACATCCATGTGGCCAATTTCATGCAGCATCGCGGGCAATATGTGGAGGTCCGGTGTATGTGAGTGACTCGGTTGATTGTCATGATTTTGATCTCATCAAGAAGTTTGCTTACCCAGATGGAACCATTCCCAAGTGCCAGCACTTTGCCCTTCCAACTAGGGACTGTCTCTTCAAAAACCCTTTGAATGACAGCAAGTCCGCTCTCAAAATTTGGAACTTGAACAAGTATGCTGGTGTCATTGGAGCTTTCAACTGCCAAGGGGCTGGCTGGGATCCAAAAGAGCAAAAGATCAGAGGCTATCCCGAATGCTATGAGGAAATCCATTGTTCAGTGCATGTTTCTGAAATTGAATGGGACCAAAAGAAAGAAGCAGCCAATTTGGGCGAGGCTGAACAGTATGTGGTCTATCTCAACCAGACTGATGAACTCCTCCTCATGACCCCCAAGTCTGATCCAATTCGAATCACAATCAAACCATCTTCGTTCGAGATTTTCACCTTTGTGCCAGTTCAAAATCTCCGCTCCAGCAGCACTAAATTCGCGCCTATTGGACTGACCAACATGTTCAACAGTGGAGGCACCATCCAAGAGTTGGAGTACAATACTAGTTCGGTTTCTTCTAGTGTCAAGATGAAGGTTAAAGGTGGAGGCAATTTCTCGGCCTACTCTAGTGAATCCCCAAAGAATTTCTGTTTGAATGGTGCTGAGGTGGATTTTGAGTGGTCTTCCTGTGGTAAACTGACCCTGAATCTTCCTTGGGTTGAAGAAGCTGCTGGAGTTTCGGATGTGGTTTTGGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

850

Amino Acids

94.5

Weight (kDa)

5.22

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 833 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 322
AasI GACNNNNNNGTC 1 cut(s) 2194
Acc36I ACCTGC 1 cut(s) 322
AccB1I GGYRCC 3 cut(s) 4, 1202, 2327
AccB7I CCANNNNNTGG 1 cut(s) 2341
AccBSI CCGCTC 2 cut(s) 1949, 2271
AccI GTMKAC 1 cut(s) 296
AccII CGCG 2 cut(s) 1780, 2291
AciI CCGC 3 cut(s) 1780, 1947, 2269
AclWI GGATC 7 cut(s) 887, 889, 900, 1672, 2015, 2028, 2195
AcoI YGGCCR 1 cut(s) 1759
AcsI RAATTY 6 cut(s) 37, 410, 701, 1956, 2285, 2434
AcuI CTGAAG 3 cut(s) 141, 1089, 1410
AfaI GTAC 2 cut(s) 475, 2348
AfiI CCNNNNNNNGG 3 cut(s) 243, 790, 2341
AflIII ACRYGT 2 cut(s) 445, 2310
AhdI GACNNNNNGTC 1 cut(s) 1942
AhlI ACTAGT 1 cut(s) 2354
AjiI CACGTC 1 cut(s) 446
AjnI CCWGG 2 cut(s) 430, 1205
AleI CACNNNNGTG 1 cut(s) 2249
AluBI AGCT 5 cut(s) 1116, 1352, 1471, 1994, 2519
AluI AGCT 5 cut(s) 1116, 1352, 1471, 1994, 2519
Alw21I GWGCWC 3 cut(s) 354, 565, 644
Alw26I GTCTC 4 cut(s) 332, 1270, 1632, 1919
Alw44I GTGCAC 1 cut(s) 640
AlwI GGATC 7 cut(s) 887, 889, 900, 1672, 2015, 2028, 2195
AoxI GGCC 5 cut(s) 552, 984, 1459, 1759, 2409
ApaLI GTGCAC 1 cut(s) 640
ApeKI GCWGC 4 cut(s) 1772, 2120, 2277, 2519
ApoI RAATTY 6 cut(s) 37, 410, 701, 1956, 2285, 2434
Asp700I GAANNNNTTC 1 cut(s) 1133
AspLEI GCGC 2 cut(s) 1229, 2293
AspS9I GGNCC 4 cut(s) 434, 984, 1796, 2104
AsuC2I CCSGG 1 cut(s) 1224
AsuHPI GGTGA 3 cut(s) 1318, 1619, 2236
AsuII TTCGAA 2 cut(s) 34, 2209
AvaII GGWCC 3 cut(s) 434, 1796, 2104
BaeGI GKGCMC 1 cut(s) 644
BalI TGGCCA 1 cut(s) 1761
BamHI GGATCC 2 cut(s) 892, 2020
BanI GGYRCC 3 cut(s) 4, 1202, 2327
BarI GAAGNNNNNNTAC 2 cut(s) 1841, 1873
BauI CACGAG 1 cut(s) 975
BbsI GAAGAC 2 cut(s) 411, 2466
Bbv12I GWGCWC 3 cut(s) 354, 565, 644
BbvCI CCTCAGC 1 cut(s) 2454
BbvI GCAGC 4 cut(s) 1784, 2132, 2289, 2506
BccI CCATC 9 cut(s) 77, 434, 512, 740, 1242, 1628, 1859, 2233, 2339
BceAI ACGGC 1 cut(s) 1446
BcgI CGANNNNNNTGC 2 cut(s) 2269, 2303
BciT130I CCWGG 2 cut(s) 432, 1207
BclI TGATCA 1 cut(s) 1345
BcnI CCSGG 1 cut(s) 1224
BcoDI GTCTC 4 cut(s) 332, 1270, 1632, 1919
BcuI ACTAGT 1 cut(s) 2354
BfaI CTAG 6 cut(s) 596, 1542, 1905, 2355, 2370, 2418
BfmI CTRYAG 2 cut(s) 556, 666
BfuAI ACCTGC 1 cut(s) 322
BisI GCNGC 4 cut(s) 1773, 2121, 2278, 2520
BlsI GCNGC 4 cut(s) 1774, 2122, 2279, 2521
Bme1390I CCNGG 3 cut(s) 432, 1207, 1224
Bme18I GGWCC 3 cut(s) 434, 1796, 2104
BmeRI GACNNNNNGTC 1 cut(s) 1942
BmgBI CACGTC 1 cut(s) 446
BmgT120I GGNCC 4 cut(s) 434, 984, 1796, 2104
BmrFI CCNGG 3 cut(s) 432, 1207, 1224
BmsI GCATC 5 cut(s) 172, 279, 490, 640, 1784
BpiI GAAGAC 2 cut(s) 411, 2466
BpmI CTGGAG 3 cut(s) 1487, 2257, 2544
Bpu10I CCTNAGC 3 cut(s) 266, 1041, 2454
Bpu14I TTCGAA 2 cut(s) 34, 2209
BpuEI CTTGAG 2 cut(s) 1168, 1454
BpuMI CCSGG 1 cut(s) 1224
BsaBI GATNNNNATC 1 cut(s) 1836
BsaI GGTCTC 1 cut(s) 1632
BsaWI WCCGGW 1 cut(s) 1798
BsaXI ACNNNNNCTCC 4 cut(s) 1398, 1428, 1785, 1815
Bsc4I CCNNNNNNNGG 3 cut(s) 243, 790, 2341
Bse118I RCCGGY 1 cut(s) 713
Bse1I ACTGG 3 cut(s) 832, 1534, 2255
Bse3DI GCAATG 2 cut(s) 110, 1564
Bse8I GATNNNNATC 1 cut(s) 1836
BseBI CCWGG 2 cut(s) 432, 1207
BseGI GGATG 9 cut(s) 655, 1060, 1177, 1339, 1710, 1716, 1750, 2331, 2539
BseJI GATNNNNATC 1 cut(s) 1836
BseLI CCNNNNNNNGG 3 cut(s) 243, 790, 2341
BseMI GCAATG 2 cut(s) 110, 1564
BseMII CTCAG 3 cut(s) 157, 453, 2445
BseNI ACTGG 3 cut(s) 832, 1534, 2255
BseRI GAGGAG 3 cut(s) 863, 2168, 2171
BseSI GKGCMC 1 cut(s) 644
BseXI GCAGC 4 cut(s) 1784, 2132, 2289, 2506
BseYI CCCAGC 1 cut(s) 2016
Bsh1236I CGCG 2 cut(s) 1780, 2291
BshFI GGCC 5 cut(s) 554, 986, 1461, 1761, 2411
BshNI GGYRCC 3 cut(s) 4, 1202, 2327
BsiHKAI GWGCWC 3 cut(s) 354, 565, 644
BsiSI CCGG 3 cut(s) 714, 1224, 1799
BslFI GGGAC 7 cut(s) 132, 596, 829, 1540, 1591, 1922, 2117
BslI CCNNNNNNNGG 3 cut(s) 243, 790, 2341
BsmAI GTCTC 4 cut(s) 332, 1270, 1632, 1919
BsmFI GGGAC 7 cut(s) 132, 596, 829, 1540, 1591, 1922, 2117
BsmI GAATGC 1 cut(s) 2060
BsnI GGCC 5 cut(s) 554, 986, 1461, 1761, 2411
Bso31I GGTCTC 1 cut(s) 1632
Bsp119I TTCGAA 2 cut(s) 34, 2209
Bsp1286I GDGCHC 3 cut(s) 354, 565, 644
Bsp19I CCATGG 3 cut(s) 287, 688, 1644
BspACI CCGC 3 cut(s) 1780, 1947, 2269
BspANI GGCC 5 cut(s) 554, 986, 1461, 1761, 2411
BspCNI CTCAG 3 cut(s) 156, 454, 2446
BspFNI CGCG 2 cut(s) 1780, 2291
BspHI TCATGA 2 cut(s) 1828, 2184
BspMI ACCTGC 1 cut(s) 322
BspPI GGATC 7 cut(s) 887, 889, 900, 1672, 2015, 2028, 2195
BspQI GCTCTTC 1 cut(s) 429
BspT104I TTCGAA 2 cut(s) 34, 2209
BspT107I GGYRCC 3 cut(s) 4, 1202, 2327
BspTNI GGTCTC 1 cut(s) 1632
BsrBI CCGCTC 2 cut(s) 1949, 2271
BsrDI GCAATG 2 cut(s) 110, 1564
BsrFI RCCGGY 1 cut(s) 713
BsrI ACTGG 3 cut(s) 832, 1534, 2255
BssAI RCCGGY 1 cut(s) 713
BssSI CACGAG 1 cut(s) 975
Bst2BI CACGAG 1 cut(s) 975
Bst2UI CCWGG 2 cut(s) 432, 1207
Bst4CI ACNGT 6 cut(s) 610, 667, 773, 1913, 2145, 2321
Bst6I CTCTTC 5 cut(s) 429, 594, 1273, 1594, 1922
BstBI TTCGAA 2 cut(s) 34, 2209
BstC8I GCNNGC 6 cut(s) 752, 1191, 1547, 1782, 1886, 2014
BstDEI CTNAG 7 cut(s) 143, 266, 355, 462, 566, 1041, 2454
BstDSI CCRYGG 4 cut(s) 287, 688, 1624, 1644
BstENI CCTNNNNNAGG 1 cut(s) 241
BstF5I GGATG 9 cut(s) 655, 1060, 1177, 1339, 1710, 1716, 1750, 2331, 2539
BstFNI CGCG 2 cut(s) 1780, 2291
BstHHI GCGC 2 cut(s) 1229, 2293
BstMAI GTCTC 4 cut(s) 332, 1270, 1632, 1919
BstMWI GCNNNNNNNGC 4 cut(s) 560, 1113, 1781, 2277
BstNI CCWGG 2 cut(s) 432, 1207
BstNSI RCATGY 5 cut(s) 1193, 1549, 1702, 2087, 2314
BstSCI CCNGG 3 cut(s) 430, 1205, 1222
BstSFI CTRYAG 2 cut(s) 556, 666
BstSLI GKGCMC 1 cut(s) 644
BstUI CGCG 2 cut(s) 1780, 2291
BstV1I GCAGC 4 cut(s) 1784, 2132, 2289, 2506
BstV2I GAAGAC 2 cut(s) 411, 2466
BstX2I RGATCY 3 cut(s) 881, 892, 2020
BstXI CCANNNNNNTGG 2 cut(s) 238, 2012
BstYI RGATCY 3 cut(s) 881, 892, 2020
BsuRI GGCC 5 cut(s) 554, 986, 1461, 1761, 2411
BtgI CCRYGG 4 cut(s) 287, 688, 1624, 1644
BtgZI GCGATG 2 cut(s) 490, 1761
BtrI CACGTC 1 cut(s) 446
BtsCI GGATG 9 cut(s) 655, 1060, 1177, 1339, 1710, 1716, 1750, 2331, 2539
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 5 cut(s) 778, 1002, 1251, 2085, 2326
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 6 cut(s) 752, 1191, 1547, 1782, 1886, 2014
CciI TCATGA 2 cut(s) 1828, 2184
CfoI GCGC 2 cut(s) 1229, 2293
Cfr10I RCCGGY 1 cut(s) 713
Cfr13I GGNCC 4 cut(s) 434, 984, 1796, 2104
CseI GACGC 1 cut(s) 803
CsiI ACCWGGT 1 cut(s) 430
Csp6I GTAC 2 cut(s) 474, 2347
CspCI CAANNNNNGTGG 2 cut(s) 1090, 1125
CviQI GTAC 2 cut(s) 474, 2347
DdeI CTNAG 7 cut(s) 143, 266, 355, 462, 566, 1041, 2454
DrdI GACNNNNNNGTC 1 cut(s) 2194
DriI GACNNNNNGTC 1 cut(s) 1942
DseDI GACNNNNNNGTC 1 cut(s) 2194
EaeI YGGCCR 1 cut(s) 1759
Eam1104I CTCTTC 5 cut(s) 429, 594, 1273, 1594, 1922
Eam1105I GACNNNNNGTC 1 cut(s) 1942
EarI CTCTTC 5 cut(s) 429, 594, 1273, 1594, 1922
Eco147I AGGCCT 1 cut(s) 554
Eco31I GGTCTC 1 cut(s) 1632
Eco32I GATATC 1 cut(s) 361
Eco47I GGWCC 3 cut(s) 434, 1796, 2104
Eco57I CTGAAG 3 cut(s) 141, 1089, 1410
EcoNI CCTNNNNNAGG 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 430, 1205
EcoRV GATATC 1 cut(s) 361
FaqI GGGAC 7 cut(s) 132, 596, 829, 1540, 1591, 1922, 2117
FauI CCCGC 1 cut(s) 1773
FauNDI CATATG 2 cut(s) 910, 1674
FbaI TGATCA 1 cut(s) 1345
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 4 cut(s) 1773, 2121, 2278, 2520
FokI GGATG 9 cut(s) 662, 1067, 1184, 1326, 1703, 1717, 1737, 2318, 2546
Fsp4HI GCNGC 4 cut(s) 1773, 2121, 2278, 2520
FspBI CTAG 6 cut(s) 596, 1542, 1905, 2355, 2370, 2418
GlaI GCGC 2 cut(s) 1228, 2292
GluI GCNGC 4 cut(s) 1773, 2121, 2278, 2520
GsaI CCCAGC 1 cut(s) 2020
GsuI CTGGAG 3 cut(s) 1487, 2257, 2544
HaeIII GGCC 5 cut(s) 554, 986, 1461, 1761, 2411
HapII CCGG 3 cut(s) 714, 1224, 1799
HgaI GACGC 1 cut(s) 803
HhaI GCGC 2 cut(s) 1229, 2293
Hin6I GCGC 2 cut(s) 1227, 2291
HinP1I GCGC 2 cut(s) 1227, 2291
HincII GTYRAC 2 cut(s) 228, 625
HindII GTYRAC 2 cut(s) 228, 625
HpaII CCGG 3 cut(s) 714, 1224, 1799
HphI GGTGA 3 cut(s) 1318, 1619, 2236
Hpy166II GTNNAC 6 cut(s) 228, 297, 625, 642, 1417, 2486
Hpy8I GTNNAC 6 cut(s) 228, 297, 625, 642, 1417, 2486
Hpy99I CGWCG 1 cut(s) 1283
HpyAV CCTTC 8 cut(s) 180, 225, 664, 915, 964, 1123, 1907, 2377
HpyCH4III ACNGT 6 cut(s) 610, 667, 773, 1913, 2145, 2321
HpyCH4IV ACGT 2 cut(s) 445, 1419
HpyCH4V TGCA 9 cut(s) 185, 331, 642, 1244, 1375, 1549, 1557, 1772, 2083
HpyF10VI GCNNNNNNNGC 4 cut(s) 560, 1113, 1781, 2277
HpyF3I CTNAG 7 cut(s) 143, 266, 355, 462, 566, 1041, 2454
HpySE526I ACGT 2 cut(s) 445, 1419
HspAI GCGC 2 cut(s) 1227, 2291
Ksp22I TGATCA 1 cut(s) 1345
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 5 cut(s) 597, 907, 1468, 1991, 2276
Lsp1109I GCAGC 4 cut(s) 1784, 2132, 2289, 2506
LweI GCATC 5 cut(s) 172, 279, 490, 640, 1784
MabI ACCWGGT 1 cut(s) 430
MaeI CTAG 6 cut(s) 596, 1542, 1905, 2355, 2370, 2418
MaeII ACGT 2 cut(s) 445, 1419
MaeIII GTNAC 9 cut(s) 136, 251, 773, 854, 977, 1000, 1249, 1659, 1811
MbiI CCGCTC 2 cut(s) 1949, 2271
MflI RGATCY 3 cut(s) 881, 892, 2020
MhlI GDGCHC 3 cut(s) 354, 565, 644
MlsI TGGCCA 1 cut(s) 1761
MluNI TGGCCA 1 cut(s) 1761
MlyI GAGTC 6 cut(s) 811, 1008, 1269, 1416, 1717, 1808
MmeI TCCRAC 8 cut(s) 152, 303, 870, 942, 1032, 1744, 1925, 2322
Mox20I TGGCCA 1 cut(s) 1761
MroXI GAANNNNTTC 1 cut(s) 1133
MscI TGGCCA 1 cut(s) 1761
MseI TTAA 4 cut(s) 24, 662, 1260, 2388
MslI CAYNNNNRTG 5 cut(s) 542, 1239, 1703, 1755, 2249
Msp20I TGGCCA 1 cut(s) 1761
MspI CCGG 3 cut(s) 714, 1224, 1799
MspR9I CCNGG 3 cut(s) 432, 1207, 1224
Mva1269I GAATGC 1 cut(s) 2060
MvaI CCWGG 2 cut(s) 432, 1207
MvnI CGCG 2 cut(s) 1780, 2291
MwoI GCNNNNNNNGC 4 cut(s) 560, 1113, 1781, 2277
NciI CCSGG 1 cut(s) 1224
NcoI CCATGG 3 cut(s) 287, 688, 1644
NdeI CATATG 2 cut(s) 910, 1674
NmeAIII GCCGAG 1 cut(s) 2387
NmuCI GTSAC 7 cut(s) 136, 773, 854, 977, 1000, 1249, 1811
NspI RCATGY 5 cut(s) 1193, 1549, 1702, 2087, 2314
NspV TTCGAA 2 cut(s) 34, 2209
OliI CACNNNNGTG 1 cut(s) 2249
PaeI GCATGC 2 cut(s) 1193, 1549
PagI TCATGA 2 cut(s) 1828, 2184
PaqCI CACCTGC 1 cut(s) 322
PceI AGGCCT 1 cut(s) 554
PciI ACATGT 1 cut(s) 2310
PciSI GCTCTTC 1 cut(s) 429
PctI GAATGC 1 cut(s) 2060
PdmI GAANNNNTTC 1 cut(s) 1133
PfeI GAWTC 4 cut(s) 914, 2211, 2423, 2497
PflMI CCANNNNNTGG 1 cut(s) 2341
PkrI GCNGC 4 cut(s) 1774, 2122, 2279, 2521
PleI GAGTC 6 cut(s) 811, 1007, 1269, 1415, 1716, 1808
PpsI GAGTC 6 cut(s) 811, 1007, 1269, 1415, 1716, 1808
PscI ACATGT 1 cut(s) 2310
Psp6I CCWGG 2 cut(s) 430, 1205
PspFI CCCAGC 1 cut(s) 2016
PspGI CCWGG 2 cut(s) 430, 1205
PspPI GGNCC 4 cut(s) 434, 984, 1796, 2104
PsuI RGATCY 3 cut(s) 881, 892, 2020
RsaI GTAC 2 cut(s) 475, 2348
RsaNI GTAC 2 cut(s) 474, 2347
RseI CAYNNNNRTG 5 cut(s) 542, 1239, 1703, 1755, 2249
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 4 cut(s) 24, 662, 1260, 2388
SatI GCNGC 4 cut(s) 1773, 2121, 2278, 2520
Sau96I GGNCC 4 cut(s) 434, 984, 1796, 2104
SchI GAGTC 6 cut(s) 811, 1008, 1269, 1416, 1717, 1808
ScrFI CCNGG 3 cut(s) 432, 1207, 1224
SduI GDGCHC 3 cut(s) 354, 565, 644
SexAI ACCWGGT 1 cut(s) 430
SfaNI GCATC 5 cut(s) 172, 279, 490, 640, 1784
SfcI CTRYAG 2 cut(s) 556, 666
SfuI TTCGAA 2 cut(s) 34, 2209
SinI GGWCC 3 cut(s) 434, 1796, 2104
SmiMI CAYNNNNRTG 5 cut(s) 542, 1239, 1703, 1755, 2249
SmlI CTYRAG 2 cut(s) 1147, 1433
SmoI CTYRAG 2 cut(s) 1147, 1433
SpeI ACTAGT 1 cut(s) 2354
SphI GCATGC 2 cut(s) 1193, 1549
SseBI AGGCCT 1 cut(s) 554
SsiI CCGC 3 cut(s) 1780, 1947, 2269
SspMI CTAG 6 cut(s) 596, 1542, 1905, 2355, 2370, 2418
StuI AGGCCT 1 cut(s) 554
StyD4I CCNGG 3 cut(s) 430, 1205, 1222
TaaI ACNGT 6 cut(s) 610, 667, 773, 1913, 2145, 2321
TaiI ACGT 2 cut(s) 448, 1422
TaqI TCGA 6 cut(s) 34, 72, 1278, 1286, 2209, 2235
TatI WGTACW 1 cut(s) 2346
TfiI GAWTC 4 cut(s) 914, 2211, 2423, 2497
Tru1I TTAA 4 cut(s) 24, 662, 1260, 2388
Tru9I TTAA 4 cut(s) 24, 662, 1260, 2388
TscAI CASTG 5 cut(s) 778, 1009, 1258, 2085, 2326
TseFI GTSAC 7 cut(s) 136, 773, 854, 977, 1000, 1249, 1811
TseI GCWGC 4 cut(s) 1772, 2120, 2277, 2519
Tsp45I GTSAC 7 cut(s) 136, 773, 854, 977, 1000, 1249, 1811
TspGWI ACGGA 2 cut(s) 1470, 1613
TspRI CASTG 5 cut(s) 778, 1009, 1258, 2085, 2326
Van91I CCANNNNNTGG 1 cut(s) 2341
VneI GTGCAC 1 cut(s) 640
VpaK11BI GGWCC 3 cut(s) 434, 1796, 2104
XagI CCTNNNNNAGG 1 cut(s) 241
XapI RAATTY 6 cut(s) 37, 410, 701, 1956, 2285, 2434
XceI RCATGY 5 cut(s) 1193, 1549, 1702, 2087, 2314
XcmI CCANNNNNNNNNTGG 1 cut(s) 2338
XmiI GTMKAC 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 1133
XspI CTAG 6 cut(s) 596, 1542, 1905, 2355, 2370, 2418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.