Rorug01G0237700

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002202
Physical Location & Seq
Reverse (-)
34246389 .. 34251906
5518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0237700.1

Sequence Viewer

Length: 4350 bp
ATGGCCGGTGCTTCTTCATCGTCTGGAGGCAGTCGCAAATACGACGTTTTCATCAATTTCAGAGGCGAAGACACACGTAGGTGTTTCATCTGCCATCTGTACGAAGCTCTGAAACAGAAAGCACTCGACACCTTCATTGATTCGGAAGAGCTTAGAAAAGGCGACGACCTTTCCGAGCTATTCAAAGCCATTCAAGACTCGAGGCTTTCGATTGTAGTTTTCTCTAAAGACTACGCATCTTCTAAATGGTGCTTAAAAGAACTGGTCCAGATCTTGGAGTGCATGGATAGCCAGAGCCAGATTGTGGTGCCCATTTTCTATGAAGTAGATCCCTCCCATATTCGCAACCTTGAGGAAAGTTTTGCGGAAGCTTTTGCAAAACATGAAAGCAATTCCAAAATCGACAAAGAAGAGTTGGAGAGCTGGAAGTCCAGTTTAAAGAGAGCCACCAATTTATCTGGCTGGGATTCCAAAAATTATCGGGATGATACCCAGCTTATCGGCAGCATTGTAGAAGACATTTTAAAGAAATTGATCCACATCTCACCAAGTAAAACCGATGGCTTGATTGGAATGGATTCTCACATGGATAAAATTGATTCGCTATTACAGCTGGGGGTGGATGAATTTCGCATTGTTGGAATCTGGGGTATGGGTGGCATCGGCAAGTCAACCATAGCTAGAGCTGTTTTTGACAGAATTGCTCATCAATTTGAACACAAGTGCTTTCTTGATAATGTGAAGCAGGGCTTCATCACAAAAAAAGGTGAAGGACAAATGGTTGAAGAACTTCTATCCAGAATTCTGAAGGTAAAGGATCGAAGCACTTTGGATGGAGGGCTGAATAAGATGAGGGAAAGACTAGGTAAGAAAAGAATTCTTCTTGTTCTTGATGATGTGGACAATCAGGACCAAATTGATACCTTGATTGGAGAAAAACCTACATTCGGTGGTGGGAGCAGAATTATTATAACAACTAGAGATAAGGCGTTACTGGGTACATGTTTCATATATGAGCCCAAGTTGTTGACTGATGAAGAAGCTCTTGAACTCTTTAGCCGGTATGCTTTCAGAAGAAACAAATATTCAGAGATTTATGATGCTATCTCAAGGCGTGCCATAGAATATGCGCATGGTCTTCCTTTAGCACTCAAAGTCTTGGGAGCTTTGCTTGTTAACAGAACTACTGTACCGGAGTGGGAATATGAGTTGGAGAAGATAAAGGAAATCCCAATCACGTCTCTTGATGATAAAATTAGACGTGTGCTTTGGGCAAGCTATGATGGATTGGATGAACGGCAGAAGAACATATTTCTAGATATTGCATGTTTCTTTAAAGGAAAGGACAAAGGTTTTGTAACAAAGTTTCATGAAAGTTGTCGCTTTTTCCCCGAGACTGGACTAAAAGTTTTGGTAGAAAGATCTCTTGTAACTGTCTCACATTATGGTACACTACAGATGCATGATTTGATACAGGAATTGGGTAGGGACATCATCTACCGAAAATCTACTGAAGATCTGGGAATGCGTAGTAGATTATGGGATTATAAAGATGTTCTCCAAGTGTTAGCTCAAAATACGGCTACGAACGTTGAAGGCATAATCCTAGACTTGTCAAAGTCAAAAGAGGTATACGTAAATGATGAAGTTTTTGTTAGCATGAGGAGACTAAGGCTACTCCAGCTTTTTTATGGAGACAAATATAGTCCGTATGGAAGTGCATGGGGAAAAGCAGGTTATGAACCCTGTGTACAATACATGAGTGAGAACTTAAAGTTTCTTTCAAATGAGTTGAGAGTTCTTATGTGGCATGGATGCCCACTAAAGTCTTTCCCAATTAATTTTCAACCGAAGAATCTTATTTATGTCGACATGTGCTATAGTCGTATTGAACAACTTTGGGAAGGAACCAAGCCTTTGCAAGACTTGGTACACATCGATTTAAGATACTCAAAGTATCTAATCAAAACCCCCGATTTCACTGGGGCAACAAATCTTAAGAAATTGAAGCTTAAAGGATGTACAAGTTTATTGGAGGTTCACCCATCCATTTCAGCCCTTAAAAGCGTCGTTGAGTTGGACCTACAAGGCTGCTCAAATCTCAAGAAGTTTCCAAATATATCCGGAAATATGAAGAGGCTAAGCGAACTTTACTTAGATGGGACTGCAATTACAGAAATGCCCTCGTCCATTAATGATCTTTTCGGGCTGGAGACTTTGAGCCTCGCTGGTTGCAGAGAACTCAGGAGTCTTCCGAGCAGCATTCACATGAAATCTCTGCAATCTCTTGATCTATCTGGCTGCTCGAAACTTACGGAGTTTCCAGAGATTTCAGAAGTAATGGAGGAGCTTTGGTTGCTTAAGTTAGACGGGACAGCAATTAAAGAACTACCCTCATCTATTAATAATCTTACGGGGCTTCGGAAGTTGAGTTTGACAAACTGCACAAGCCTTGTCTGTCTTCCGGACAATATCTGCAATTTGGCAAGCCTTAGTGGTCTCAATCTCTATCTGACTGGGTGCTCAAGCCTTGTCTGTCTTCCGGACAATATCTGCAATTTGGCATGCCTTTATGAACTCAATCTGACTGGGTGCTCAAGCCTTGTCTGTCTTCCGGACAATATCTGCAATTTGGCATGCCTTTATGAACTCAATCTGACTGGGTGCTCAAGCCTTGTCTGTCTTCCGGACAATATCTGCAATTTGGCACGCCTTCGTTTTCTCTATCTGACTGGGTGCTCAAGCCTTGTCTGTCTTCCGGACAATATCTGCAATTTGGCACGCCTTTTTCATCTCAATCTGACTGGGTGCTCAAGCCTTGTCTGTCTTCCGGACAATATCTGCAATTTGCCAAGCCTTAGTGATCTCAATCTGACTGGGTGCTCAAGCCTTGTCTGTCTTCCTGACAATATCTGCAATTTGCCAAGCCTTTCTCATCTCAATCTGACTGGGTGTTCAAGCCTTGTCTGTCTTCCGGACAATATCTGCAATTTGCCAAGCCTTAGTGGTCTCAATCTCAATCTGACTGGGTGCTCTAACCTTTCAAATTTTTACTTTGGGAATTTGAAATTCTTGTGGCAATTTAAAGTGGAGAGAAGTGGTATAAAACAACTTAATGGGTGTGGGCAATCATTATCATGTAATGGATGTAAAGAGATGACGGCGCCCTTTTCCTCGTGGCCAACATGGAACCTCCAAAATCAGAGGAACTCTTTTCTGCGCAATCGCTTGGAATTATCAGATGGCATTGCTCATTTAGATTCATTGTATGAATTAAACTTATCCGGAAACAACAAGTTGGAAAGTTTACCTGCAGCAATGAATCGCCTGGGTAAATTAAGACGGCTCTATCTGAGAGGTTGCAAGAGACTGAAATCAATACCAGAGCTTTCATCGAGTATACAAGAAATTGATGCCCAAGATTGCACGTCTTTGGAGACAGTTTCAACACCACAGCCTCCGTACGCTGAGGACATTCGCTTCCGATTTGTTAATTGCTTCAAGTTGGTGATCAATACCAATCTATTTATAGATATTGTGGAAACTGCTACTGATATTCAGGTTATTTATGATCGTTATATTGGACCTCCTTGCCATGTATCTCTTCCTGGAAGTGATATTCCAGAATGGTTCAACCATCAGAGTAGAGGGTCTTCCGTAACTGTGCAATTACCACCCAATTGGCTCGCAAACAAGTTTCTAGGGTTCGCTATATGCGCCATCGGTAACTTGCGGGGTGCTGCAAATACAAAAACTCAATCTGCTGTATGTTGTTGTACCTTAGAAGCAAATGATGGTCAGGACAATTTCTATTTTTCTTTGTTCGATATTCTAGATGGAACTGGCATGCCTCTTGAATCAGATCATATGTTGCTGGGATATACAAATTTCCGAAGTTTAGGAGAGAGGCATAATGAACGCAAATACACCGAGGCCAGATTTAGGATAGTGGCAAGCACCTATGGAGTCATCCCAAATGATTCAATGACATTAGAAGATTGCACTTGGATCACAAGTTGCGGGGTTCGGTTATTCCACATAAATCCACCACCCTCTGTGGACCTTATTTGCAATTTGGAAAATAGAGAAACAGAGATAAGGAGTAAGAGAAAGAGAGAAACACTAGAGAGAGGGAAGGAGAAGATGGGGGTGGAGGTGCAGGAAGAGGATGAGGAGTGCTCAGGAGGGCCCATATGCTACTGCTTCTCGTTGAGTGGGTGCACAATATTGTTGAGAAAGCGACAAAGAGAAAAAGAAAGAGAGGGAGAGGGTGTTGCTGGAGACACAGGATTAGAGTCAGAGGACGCGGATGTCTCAGGGGAAGGGGGACTCCTCACATTGTCTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

1449

Amino Acids

162.68

Weight (kDa)

5.74

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 13 - 188 7.1e-51 TIR domain
TIR_2 PF13676 16 - 111 2.6e-14 TIR domain
NB-ARC PF00931 194 - 360 5.6e-22 NB-ARC domain
WHD_ROQ1 PF23282 432 - 502 1.2e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 679 - 784 1e-06 Leucine-rich repeat region
LRR_13 PF23286 688 - 757 9e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 757 - 844 6.1e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 759 - 865 2.1e-08 Leucine-rich repeat region
LRR_14 PF23598 836 - 937 2.7e-07 Leucine-rich repeat region
LRR_14 PF23598 905 - 1009 9.3e-09 Leucine-rich repeat region
LRR_13 PF23286 966 - 1145 8.1e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1202 - 1347 5.9e-21 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 971, 1548
AasI GACNNNNNNGTC 1 cut(s) 4310
Acc16I TGCGCA 2 cut(s) 1131, 3221
Acc36I ACCTGC 2 cut(s) 1724, 3319
AccB1I GGYRCC 2 cut(s) 307, 3163
AccB7I CCANNNNNTGG 2 cut(s) 274, 304
AccI GTMKAC 3 cut(s) 1632, 1869, 3400
AccII CGCG 1 cut(s) 4307
AccIII TCCGGA 9 cut(s) 2123, 2464, 2542, 2614, 2686, 2758, 2830, 2974, 3284
AciI CCGC 4 cut(s) 365, 3733, 4020, 4307
AclI AACGTT 1 cut(s) 1590
AclWI GGATC 4 cut(s) 323, 529, 825, 4016
AcoI YGGCCR 2 cut(s) 3, 3179
AcsI RAATTY 7 cut(s) 626, 801, 876, 3046, 3061, 3068, 3886
AcuI CTGAAG 2 cut(s) 827, 1533
AcyI GRCGYC 1 cut(s) 3164
AfaI GTAC 9 cut(s) 101, 1000, 1191, 1450, 1752, 1932, 2023, 3464, 3778
AfiI CCNNNNNNNGG 5 cut(s) 274, 304, 947, 1397, 3942
AflII CTTAAG 2 cut(s) 1997, 2360
AflIII ACRYGT 4 cut(s) 74, 1001, 1261, 1872
AjiI CACGTC 3 cut(s) 1239, 1262, 3429
AjnI CCWGG 2 cut(s) 3327, 3607
AleI CACNNNNGTG 1 cut(s) 79
Alw21I GWGCWC 9 cut(s) 2525, 2597, 2669, 2741, 2813, 2885, 3035, 4181, 4223
Alw44I GTGCAC 1 cut(s) 4219
AlwI GGATC 4 cut(s) 323, 529, 825, 4016
Ama87I CYCGRG 2 cut(s) 199, 1391
Aor13HI TCCGGA 9 cut(s) 2123, 2464, 2542, 2614, 2686, 2758, 2830, 2974, 3284
AoxI GGCC 4 cut(s) 3, 3179, 3933, 4187
ApaI GGGCCC 1 cut(s) 4191
ApaLI GTGCAC 1 cut(s) 4219
ApeKI GCWGC 6 cut(s) 504, 2091, 2259, 2301, 3314, 3740
ApoI RAATTY 7 cut(s) 626, 801, 876, 3046, 3061, 3068, 3886
ArsI GACNNNNNNTTYG 4 cut(s) 1251, 1283, 1337, 1369
AseI ATTAAT 3 cut(s) 1839, 2193, 2403
Asp700I GAANNNNTTC 2 cut(s) 577, 789
AspLEI GCGC 4 cut(s) 1132, 3166, 3222, 3719
AspS9I GGNCC 7 cut(s) 265, 910, 2080, 3584, 4060, 4187, 4188
AsuHPI GGTGA 4 cut(s) 537, 779, 2033, 3520
AvaI CYCGRG 2 cut(s) 199, 1391
AvaII GGWCC 5 cut(s) 265, 910, 2080, 3584, 4060
BaeGI GKGCMC 3 cut(s) 312, 4191, 4223
BaeI ACNNNNGTAYC 2 cut(s) 1173, 1206
BalI TGGCCA 1 cut(s) 3181
BanI GGYRCC 2 cut(s) 307, 3163
BanII GRGCYC 2 cut(s) 1020, 4191
BarI GAAGNNNNNNTAC 2 cut(s) 3637, 3669
BauI CACGAG 1 cut(s) 3175
Bbv12I GWGCWC 9 cut(s) 2525, 2597, 2669, 2741, 2813, 2885, 3035, 4181, 4223
BbvCI CCTCAGC 1 cut(s) 3468
BbvI GCAGC 6 cut(s) 516, 2078, 2271, 2288, 3326, 3727
BceAI ACGGC 4 cut(s) 1313, 1596, 3177, 3359
BciT130I CCWGG 2 cut(s) 3329, 3609
BclI TGATCA 1 cut(s) 3510
BfaI CTAG 9 cut(s) 681, 863, 978, 1316, 1607, 3701, 3833, 4124, 4348
BfmI CTRYAG 3 cut(s) 1454, 1879, 3312
BfoI RGCGCY 1 cut(s) 3167
BfrI CTTAAG 2 cut(s) 1997, 2360
BfuAI ACCTGC 2 cut(s) 1724, 3319
BglII AGATCT 3 cut(s) 270, 1421, 1516
BisI GCNGC 6 cut(s) 505, 2092, 2260, 2302, 3315, 3741
BlpI GCTNAGC 1 cut(s) 2141
BlsI GCNGC 6 cut(s) 506, 2093, 2261, 2303, 3316, 3742
Bme1390I CCNGG 2 cut(s) 3329, 3609
Bme18I GGWCC 5 cut(s) 265, 910, 2080, 3584, 4060
BmeT110I CYCGRG 2 cut(s) 199, 1391
BmgBI CACGTC 3 cut(s) 1239, 1262, 3429
BmgT120I GGNCC 7 cut(s) 265, 910, 2080, 3584, 4060, 4187, 4188
BmiI GGNNCC 5 cut(s) 309, 1909, 3165, 3191, 4189
BmrFI CCNGG 2 cut(s) 3329, 3609
BmsI GCATC 6 cut(s) 245, 669, 1090, 1449, 1805, 3403
BplI GAGNNNNNCTC 2 cut(s) 4163, 4195
BpmI CTGGAG 4 cut(s) 45, 1664, 2231, 4299
Bpu10I CCTNAGC 2 cut(s) 3468, 4180
Bpu1102I GCTNAGC 1 cut(s) 2141
BpuEI CTTGAG 9 cut(s) 371, 1093, 2087, 2509, 2581, 2653, 2725, 2797, 2869
Bsa29I ATCGAT 1 cut(s) 1938
BsaAI YACGTR 2 cut(s) 77, 1636
BsaBI GATNNNNATC 2 cut(s) 539, 2868
BsaHI GRCGYC 1 cut(s) 3164
BsaI GGTCTC 2 cut(s) 2504, 3014
BsaJI CCNNGG 2 cut(s) 3328, 3930
BsaXI ACNNNNNCTCC 4 cut(s) 2339, 2369, 4257, 4287
Bsc4I CCNNNNNNNGG 5 cut(s) 274, 304, 947, 1397, 3942
Bse118I RCCGGY 2 cut(s) 5, 1059
Bse3DI GCAATG 2 cut(s) 3246, 3324
Bse8I GATNNNNATC 2 cut(s) 539, 2868
BseAI TCCGGA 9 cut(s) 2123, 2464, 2542, 2614, 2686, 2758, 2830, 2974, 3284
BseBI CCWGG 2 cut(s) 3329, 3609
BseCI ATCGAT 1 cut(s) 1938
BseDI CCNNGG 2 cut(s) 3328, 3930
BseJI GATNNNNATC 2 cut(s) 539, 2868
BseLI CCNNNNNNNGG 5 cut(s) 274, 304, 947, 1397, 3942
BseMI GCAATG 2 cut(s) 3246, 3324
BseMII CTCAG 5 cut(s) 2257, 3344, 3459, 4194, 4329
BseRI GAGGAG 4 cut(s) 1678, 2360, 4187, 4322
BseSI GKGCMC 3 cut(s) 312, 4191, 4223
BseXI GCAGC 6 cut(s) 516, 2078, 2271, 2288, 3326, 3727
BseYI CCCAGC 4 cut(s) 462, 492, 613, 3874
BsgI GTGCAG 2 cut(s) 2428, 4178
Bsh1236I CGCG 1 cut(s) 4307
BshFI GGCC 4 cut(s) 5, 3181, 3935, 4189
BshNI GGYRCC 2 cut(s) 307, 3163
BshVI ATCGAT 1 cut(s) 1938
BsiHKAI GWGCWC 9 cut(s) 2525, 2597, 2669, 2741, 2813, 2885, 3035, 4181, 4223
BsiHKCI CYCGRG 2 cut(s) 199, 1391
BsiWI CGTACG 1 cut(s) 3462
BslFI GGGAC 4 cut(s) 1502, 2176, 2386, 4341
BslI CCNNNNNNNGG 5 cut(s) 274, 304, 947, 1397, 3942
BsmBI CGTCTC 1 cut(s) 1245
BsmFI GGGAC 4 cut(s) 1502, 2176, 2386, 4341
BsmI GAATGC 2 cut(s) 1530, 2262
BsnI GGCC 4 cut(s) 5, 3181, 3935, 4189
Bso31I GGTCTC 2 cut(s) 2504, 3014
BsoBI CYCGRG 2 cut(s) 199, 1391
Bsp120I GGGCCC 1 cut(s) 4187
Bsp13I TCCGGA 9 cut(s) 2123, 2464, 2542, 2614, 2686, 2758, 2830, 2974, 3284
Bsp1407I TGTACA 2 cut(s) 1750, 2021
Bsp1720I GCTNAGC 1 cut(s) 2141
BspACI CCGC 4 cut(s) 365, 3733, 4020, 4307
BspANI GGCC 4 cut(s) 5, 3181, 3935, 4189
BspCNI CTCAG 5 cut(s) 2256, 3345, 3460, 4193, 4328
BspDI ATCGAT 1 cut(s) 1938
BspEI TCCGGA 9 cut(s) 2123, 2464, 2542, 2614, 2686, 2758, 2830, 2974, 3284
BspFNI CGCG 1 cut(s) 4307
BspHI TCATGA 1 cut(s) 1369
BspLI GGNNCC 5 cut(s) 309, 1909, 3165, 3191, 4189
BspMAI CTGCAG 1 cut(s) 3316
BspMI ACCTGC 2 cut(s) 1724, 3319
BspPI GGATC 4 cut(s) 323, 529, 825, 4016
BspQI GCTCTTC 1 cut(s) 141
BspT107I GGYRCC 2 cut(s) 307, 3163
BspTI CTTAAG 2 cut(s) 1997, 2360
BspTNI GGTCTC 2 cut(s) 2504, 3014
BsrDI GCAATG 2 cut(s) 3246, 3324
BsrFI RCCGGY 2 cut(s) 5, 1059
BsrGI TGTACA 2 cut(s) 1750, 2021
BssAI RCCGGY 2 cut(s) 5, 1059
BssECI CCNNGG 2 cut(s) 3328, 3930
BssNAI GTATAC 2 cut(s) 1633, 3401
BssNI GRCGYC 1 cut(s) 3164
BssSI CACGAG 1 cut(s) 3175
Bst1107I GTATAC 2 cut(s) 1633, 3401
Bst2BI CACGAG 1 cut(s) 3175
Bst2UI CCWGG 2 cut(s) 3329, 3609
Bst4CI ACNGT 4 cut(s) 1189, 1435, 3442, 3664
Bst6I CTCTTC 5 cut(s) 141, 405, 2129, 3609, 4158
BstACI GRCGYC 1 cut(s) 3164
BstAFI CTTAAG 2 cut(s) 1997, 2360
BstAUI TGTACA 2 cut(s) 1750, 2021
BstBAI YACGTR 2 cut(s) 77, 1636
BstFNI CGCG 1 cut(s) 4307
BstH2I RGCGCY 1 cut(s) 3167
BstHHI GCGC 4 cut(s) 1132, 3166, 3222, 3719
BstMWI GCNNNNNNNGC 5 cut(s) 288, 610, 1681, 2356, 3716
BstNI CCWGG 2 cut(s) 3329, 3609
BstNSI RCATGY 6 cut(s) 1005, 1329, 1876, 2568, 2640, 3850
BstSCI CCNGG 2 cut(s) 3327, 3607
BstSFI CTRYAG 3 cut(s) 1454, 1879, 3312
BstSLI GKGCMC 3 cut(s) 312, 4191, 4223
BstSNI TACGTA 1 cut(s) 1636
BstUI CGCG 1 cut(s) 4307
BstV1I GCAGC 6 cut(s) 516, 2078, 2271, 2288, 3326, 3727
BstX2I RGATCY 4 cut(s) 270, 328, 1421, 1516
BstXI CCANNNNNNTGG 1 cut(s) 3681
BstYI RGATCY 4 cut(s) 270, 328, 1421, 1516
BstZ17I GTATAC 2 cut(s) 1633, 3401
Bsu15I ATCGAT 1 cut(s) 1938
BsuRI GGCC 4 cut(s) 5, 3181, 3935, 4189
BsuTUI ATCGAT 1 cut(s) 1938
BtrI CACGTC 3 cut(s) 1239, 1262, 3429
BtsIMutI CAGTG 1 cut(s) 1980
BveI ACCTGC 2 cut(s) 1724, 3319
CciI TCATGA 1 cut(s) 1369
CfoI GCGC 4 cut(s) 1132, 3166, 3222, 3719
Cfr10I RCCGGY 2 cut(s) 5, 1059
Cfr13I GGNCC 7 cut(s) 265, 910, 2080, 3584, 4060, 4187, 4188
ClaI ATCGAT 1 cut(s) 1938
CseI GACGC 2 cut(s) 2056, 4313
Csp6I GTAC 9 cut(s) 100, 999, 1190, 1449, 1751, 1931, 2022, 3463, 3777
CviQI GTAC 9 cut(s) 100, 999, 1190, 1449, 1751, 1931, 2022, 3463, 3777
DinI GGCGCC 1 cut(s) 3165
DraI TTTAAA 4 cut(s) 438, 525, 1336, 3085
DrdI GACNNNNNNGTC 1 cut(s) 4310
DseDI GACNNNNNNGTC 1 cut(s) 4310
EaeI YGGCCR 2 cut(s) 3, 3179
Eam1104I CTCTTC 5 cut(s) 141, 405, 2129, 3609, 4158
EarI CTCTTC 5 cut(s) 141, 405, 2129, 3609, 4158
Eco105I TACGTA 1 cut(s) 1636
Eco24I GRGCYC 2 cut(s) 1020, 4191
Eco31I GGTCTC 2 cut(s) 2504, 3014
Eco47I GGWCC 5 cut(s) 265, 910, 2080, 3584, 4060
Eco57I CTGAAG 2 cut(s) 827, 1533
Eco88I CYCGRG 2 cut(s) 199, 1391
EcoO109I RGGNCCY 1 cut(s) 4187
EcoRI GAATTC 2 cut(s) 801, 876
EcoRII CCWGG 2 cut(s) 3327, 3607
EcoT22I ATGCAT 1 cut(s) 1464
EcoT38I GRGCYC 2 cut(s) 1020, 4191
EgeI GGCGCC 1 cut(s) 3165
EheI GGCGCC 1 cut(s) 3165
Esp3I CGTCTC 1 cut(s) 1245
FalI AAGNNNNNCTT 4 cut(s) 734, 766, 1029, 1061
FaqI GGGAC 4 cut(s) 1502, 2176, 2386, 4341
FauI CCCGC 2 cut(s) 3726, 4013
FauNDI CATATG 2 cut(s) 3867, 4193
FbaI TGATCA 1 cut(s) 3510
FblI GTMKAC 3 cut(s) 1632, 1869, 3400
Fnu4HI GCNGC 6 cut(s) 505, 2092, 2260, 2302, 3315, 3741
FriOI GRGCYC 2 cut(s) 1020, 4191
Fsp4HI GCNGC 6 cut(s) 505, 2092, 2260, 2302, 3315, 3741
FspAI RTGCGCAY 1 cut(s) 1131
FspBI CTAG 9 cut(s) 681, 863, 978, 1316, 1607, 3701, 3833, 4124, 4348
FspI TGCGCA 2 cut(s) 1131, 3221
GlaI GCGC 4 cut(s) 1131, 3165, 3221, 3718
GluI GCNGC 6 cut(s) 505, 2092, 2260, 2302, 3315, 3741
GsaI CCCAGC 4 cut(s) 466, 496, 617, 3878
GsuI CTGGAG 4 cut(s) 45, 1664, 2231, 4299
HaeII RGCGCY 1 cut(s) 3167
HaeIII GGCC 4 cut(s) 5, 3181, 3935, 4189
HgaI GACGC 2 cut(s) 2056, 4313
HhaI GCGC 4 cut(s) 1132, 3166, 3222, 3719
Hin1I GRCGYC 1 cut(s) 3164
Hin6I GCGC 4 cut(s) 1130, 3164, 3220, 3717
HinP1I GCGC 4 cut(s) 1130, 3164, 3220, 3717
HincII GTYRAC 4 cut(s) 672, 1029, 1177, 1870
HindII GTYRAC 4 cut(s) 672, 1029, 1177, 1870
HindIII AAGCTT 2 cut(s) 369, 2009
HpaI GTTAAC 1 cut(s) 1177
HphI GGTGA 4 cut(s) 537, 779, 2033, 3520
Hpy99I CGWCG 3 cut(s) 47, 167, 2072
HpyAV CCTTC 8 cut(s) 142, 764, 802, 1589, 1898, 2723, 4129, 4316
HpyCH4III ACNGT 4 cut(s) 1189, 1435, 3442, 3664
HpyCH4IV ACGT 7 cut(s) 45, 76, 1238, 1261, 1590, 1635, 3428
HpyF10VI GCNNNNNNNGC 5 cut(s) 288, 610, 1681, 2356, 3716
HpySE526I ACGT 7 cut(s) 45, 76, 1238, 1261, 1590, 1635, 3428
Hsp92I GRCGYC 1 cut(s) 3164
HspAI GCGC 4 cut(s) 1130, 3164, 3220, 3717
KasI GGCGCC 1 cut(s) 3163
Kpn2I TCCGGA 9 cut(s) 2123, 2464, 2542, 2614, 2686, 2758, 2830, 2974, 3284
Ksp22I TGATCA 1 cut(s) 3510
KspAI GTTAAC 1 cut(s) 1177
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 3 cut(s) 957, 1163, 2347
Lsp1109I GCAGC 6 cut(s) 516, 2078, 2271, 2288, 3326, 3727
LweI GCATC 6 cut(s) 245, 669, 1090, 1449, 1805, 3403
MaeI CTAG 9 cut(s) 681, 863, 978, 1316, 1607, 3701, 3833, 4124, 4348
MaeII ACGT 7 cut(s) 45, 76, 1238, 1261, 1590, 1635, 3428
MaeIII GTNAC 5 cut(s) 990, 1357, 1429, 3658, 3725
MfeI CAATTG 1 cut(s) 3679
MflI RGATCY 4 cut(s) 270, 328, 1421, 1516
MlsI TGGCCA 1 cut(s) 3181
MluNI TGGCCA 1 cut(s) 3181
Mly113I GGCGCC 1 cut(s) 3164
MlyI GAGTC 5 cut(s) 191, 2257, 3975, 4304, 4323
MmeI TCCRAC 5 cut(s) 396, 619, 1191, 2058, 3279
Mox20I TGGCCA 1 cut(s) 3181
Mph1103I ATGCAT 1 cut(s) 1464
MroI TCCGGA 9 cut(s) 2123, 2464, 2542, 2614, 2686, 2758, 2830, 2974, 3284
MroXI GAANNNNTTC 2 cut(s) 577, 789
MscI TGGCCA 1 cut(s) 3181
MslI CAYNNNNRTG 3 cut(s) 79, 2267, 3136
Msp20I TGGCCA 1 cut(s) 3181
MspA1I CMGCKG 1 cut(s) 613
MspCI CTTAAG 2 cut(s) 1997, 2360
MspR9I CCNGG 2 cut(s) 3329, 3609
MunI CAATTG 1 cut(s) 3679
Mva1269I GAATGC 2 cut(s) 1530, 2262
MvaI CCWGG 2 cut(s) 3329, 3609
MvnI CGCG 1 cut(s) 4307
MwoI GCNNNNNNNGC 5 cut(s) 288, 610, 1681, 2356, 3716
NarI GGCGCC 1 cut(s) 3164
NdeI CATATG 2 cut(s) 3867, 4193
NlaIV GGNNCC 5 cut(s) 309, 1909, 3165, 3191, 4189
NsbI TGCGCA 2 cut(s) 1131, 3221
NsiI ATGCAT 1 cut(s) 1464
NspI RCATGY 6 cut(s) 1005, 1329, 1876, 2568, 2640, 3850
OliI CACNNNNGTG 1 cut(s) 79
PaeI GCATGC 3 cut(s) 2568, 2640, 3850
PaeR7I CTCGAG 1 cut(s) 199
PagI TCATGA 1 cut(s) 1369
PciI ACATGT 2 cut(s) 1001, 1872
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 206
PctI GAATGC 2 cut(s) 1530, 2262
PdmI GAANNNNTTC 2 cut(s) 577, 789
Pfl23II CGTACG 1 cut(s) 3462
PflMI CCANNNNNTGG 2 cut(s) 274, 304
PfoI TCCNGGA 1 cut(s) 3607
PkrI GCNGC 6 cut(s) 506, 2093, 2261, 2303, 3316, 3742
PleI GAGTC 5 cut(s) 191, 2256, 3974, 4303, 4323
PluTI GGCGCC 1 cut(s) 3167
PpsI GAGTC 5 cut(s) 191, 2256, 3974, 4303, 4323
Ppu21I YACGTR 2 cut(s) 77, 1636
PscI ACATGT 2 cut(s) 1001, 1872
PshBI ATTAAT 3 cut(s) 1839, 2193, 2403
PsiI TTATAA 2 cut(s) 971, 1548
Psp1406I AACGTT 1 cut(s) 1590
Psp6I CCWGG 2 cut(s) 3327, 3607
PspFI CCCAGC 4 cut(s) 462, 492, 613, 3874
PspGI CCWGG 2 cut(s) 3327, 3607
PspLI CGTACG 1 cut(s) 3462
PspN4I GGNNCC 5 cut(s) 309, 1909, 3165, 3191, 4189
PspOMI GGGCCC 1 cut(s) 4187
PspPI GGNCC 7 cut(s) 265, 910, 2080, 3584, 4060, 4187, 4188
PspXI VCTCGAGB 1 cut(s) 199
PsrI GAACNNNNNNTAC 2 cut(s) 1734, 1766
PstI CTGCAG 1 cut(s) 3316
PsuI RGATCY 4 cut(s) 270, 328, 1421, 1516
PvuII CAGCTG 1 cut(s) 613
RsaI GTAC 9 cut(s) 101, 1000, 1191, 1450, 1752, 1932, 2023, 3464, 3778
RsaNI GTAC 9 cut(s) 100, 999, 1190, 1449, 1751, 1931, 2022, 3463, 3777
RseI CAYNNNNRTG 3 cut(s) 79, 2267, 3136
SalI GTCGAC 1 cut(s) 1868
SapI GCTCTTC 1 cut(s) 141
SatI GCNGC 6 cut(s) 505, 2092, 2260, 2302, 3315, 3741
Sau96I GGNCC 7 cut(s) 265, 910, 2080, 3584, 4060, 4187, 4188
SchI GAGTC 5 cut(s) 191, 2257, 3975, 4304, 4323
ScrFI CCNGG 2 cut(s) 3329, 3609
SfaNI GCATC 6 cut(s) 245, 669, 1090, 1449, 1805, 3403
SfcI CTRYAG 3 cut(s) 1454, 1879, 3312
SfoI GGCGCC 1 cut(s) 3165
Sfr274I CTCGAG 1 cut(s) 199
SinI GGWCC 5 cut(s) 265, 910, 2080, 3584, 4060
SlaI CTCGAG 1 cut(s) 199
SmiMI CAYNNNNRTG 3 cut(s) 79, 2267, 3136
SnaBI TACGTA 1 cut(s) 1636
SphI GCATGC 3 cut(s) 2568, 2640, 3850
SsiI CCGC 4 cut(s) 365, 3733, 4020, 4307
SspDI GGCGCC 1 cut(s) 3163
SspI AATATT 2 cut(s) 1085, 4227
SspMI CTAG 9 cut(s) 681, 863, 978, 1316, 1607, 3701, 3833, 4124, 4348
StyD4I CCNGG 2 cut(s) 3327, 3607
TaaI ACNGT 4 cut(s) 1189, 1435, 3442, 3664
TaiI ACGT 7 cut(s) 48, 79, 1241, 1264, 1593, 1638, 3431
TatI WGTACW 2 cut(s) 1750, 2021
TscAI CASTG 1 cut(s) 1987
TseI GCWGC 6 cut(s) 504, 2091, 2259, 2301, 3314, 3740
TspGWI ACGGA 4 cut(s) 1698, 2330, 3450, 3646
TspRI CASTG 1 cut(s) 1987
Van91I CCANNNNNTGG 2 cut(s) 274, 304
Vha464I CTTAAG 2 cut(s) 1997, 2360
VneI GTGCAC 1 cut(s) 4219
VpaK11BI GGWCC 5 cut(s) 265, 910, 2080, 3584, 4060
VspI ATTAAT 3 cut(s) 1839, 2193, 2403
XapI RAATTY 7 cut(s) 626, 801, 876, 3046, 3061, 3068, 3886
XbaI TCTAGA 2 cut(s) 1315, 3832
XceI RCATGY 6 cut(s) 1005, 1329, 1876, 2568, 2640, 3850
XcmI CCANNNNNNNNNTGG 1 cut(s) 1688
XhoI CTCGAG 1 cut(s) 199
XmiI GTMKAC 3 cut(s) 1632, 1869, 3400
XmnI GAANNNNTTC 2 cut(s) 577, 789
XspI CTAG 9 cut(s) 681, 863, 978, 1316, 1607, 3701, 3833, 4124, 4348
Zsp2I ATGCAT 1 cut(s) 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.