Rw1G021790

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002202
Physical Location & Seq
Reverse (-)
46173423 .. 46176339
2917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021790.1

Sequence Viewer

Length: 2613 bp
ATGGCACCTCCTAATATTCCTGTTAATCCAGTCCTCAATGTTATCCGGTCTGAGAGTTCAGAAAAATACTTTGATTTGTCTGATGGAAAGTTTAATGTCAAAGGTGTTCCATTGCTCTCTGAAGTTCCAAGCAATGTCACCTTTAGTCCTTTTTCTTCAATCTGCCAATCTTCTTCTGATGTTCCAACTCATCTGCTCAGCCGTGTCAGTGCCTTGTCCCACAAGGCAGGGTTCCTTGGTTTCAACAAGGAGTCGTCTTCTGATAAGTTGACAAATTCATTGGGAAAATTCGATAACAGGGATTTCCTTAGTATCTTTAGGTTCAAAACATGGTGGTCTACTATGTGGGTTGGGAACTCTGGGTCAGATTTGCAAATGGAAACCCAATGGGTGCTCCTAAATGTTCCAGAAATCAATTCCTATGTCATAATCATACCCATCATCGATGGCAGTTTTAGATCTGCTTTTCAACCTGGTACTGATGGTCATGTCATGATCTTTGCTGAGAGTGGTTCTACCCAAGTGAAAGCATCGAAGTTTGATGCCATCGCTTATGTTCATGTGTCCGACAATCCCTACACCTTGATGAAAGAGGCCTACAGTGCTCTGAGGGTCCATCTCAATACCTTTAGTCTCCTTGAAGAAAAATCCGCACCAAATCTAGTTGATAAATTTGGTTGGTGCACTTGGGATGCCTTCTACTTAACTGTGGAACCTGTTGGTGTCTCCCATGGTGTAAATGAATTCGTTGAGGCTGGTGTACCCCCAAGGTTTCTTATCATTGATGATGGTTGGCAAAGCATTAATTTCGATGATGATGAGAACCCGAATGAGGATGCGAAGAATCTAGTCATTGGTGGGACTCAAATGACTGCTAGGCTTCACAGGCTTGAGGAGTGTGATAAGTTCAAAAACTACAAAGGTGGATCTTTACTGGGTCCTCATGCACCTTCATTTGATCCAAAAAGGCCAAAGATGTTGATATCCAAGGCAATTGAATTAGAGAATGTTGAAAAGGCTCGTGATAAGGCCATTCAATCTGGAGTGACTGATTTGTCCGAGTTCCAAAAACAAATGCAGAAGCATAAACAAGAGTTGGATATGATAATTGGTGGTGGAGAAGAAGGCAGTGTTTCGAAAGAAGGAAGCTGTAGAAGCTGTTCTTGCAAGGCTAAAAATTATGGAATGAAGTCTTTCACATGCGACTTGAGAACCAAGTTCAAGAATTTGGATGATATTTATGTATGGCATGCTCTTTGTGGTGCCTGGGGTGGTGTTAAACCAGGTACAACTCATCTCAATTCAAAGATCATCCCCTGCAAAGTCTCTCCGGGACTAGATGGTTCCATGTCTGACCTAGCTGTGGTGAAACTAGTTGAGGGTGGGATTGGACTTGTCCATCCTGATCAAGCTGGTGATTTCTATAATTCTATGCACTCTTACCTTTCTGAAGCAGGTATCACAGGAGTCAAAGTGGATGTTATTCATACTCTTGAATATGTATCTGAGGAATTCGGAGGCCGTGTTGAGCTCGCAAAGGCTTATTACAAGGGGCTGACTTCTTCTCTTGTCAAGAACTTCAATGGAACTGGACTTATTGCTAGCATGCAACAATGCAATGATTTCTTCTTCCTTGGAACAAAACAAATCTCTATTGGAAGAGTTGGTGATGATTTTTGGTTTCAGGATCCAAATGGAGACCCCATGGGAGTTTACTGGCTACAAGGTGTCCATATGATCCATTGTGCCTACAACAGCATGTGGATGGGTCAATTCATTCAGCCTGATTGGGATATGTTCCAGTCAGATCATCTGTGTGCCAAATTTCATGCCGGATCTAGGGCTATATGTGGAGGTCCGGTGTATATAAGTGACTCGGTTGGTGGTCACAATTTTGATCTTATTAGGCAGCTTGTTTACCCAGATGGTACCATCCCCAAGTGCCAGCATTTTGCTCTTCCAACTAGGGACTGCCTATTCAAGAACCCCTTATTTGACAGCAAGACTGCTCTCAAAATTTGGAATGTCAACAAGTTTGGTGGTGTGATGGGAGCTTTCAACTGCCAAGGGGCTGGTTGGGACCCAAAAGAAAAGAGAATCAAAGGCTACTCCCAGTGCTACAAAGAAATCCTCTATTCAGTGCATGTTTCTGAAATTGAATGGGACCAAAAGATGGAAGCCGCCCATTTGGGTAAGGCCGAAGAATATGTGGTCTATCTCAACCAGGCCAATGAACTCTTCCAAATGACCCCCAAATCCGATCCCATTCAAATGGTGATCCAACCATCTTCGTTCGAGATTTTCAGCTTCGTGCCAGTACAAAAGCTCGGCTCCAACAACACCAAGTTTGCGCCGATTGGACTGACAAACATGTTCAACAGTGGAGGCACTATCCAAGAGCTGGAGTATACAAGTGGCATGGAATCTTATAGTGCAAAGATCAAGGTTAAAGGTGGAGGCAACTTCTTGGCCTACTCAAGTCAGTCTCCGAAGAAGTGTTGTTTGAATGGTGCTGTAGTGACTTTTGAGTGGTCTGCTTCTGGTAAACTGTCCCTGAATCTTCCTTGGGTTGAAGAAGCTGCTGGAATTTCTGATGTCGTTCTTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

870

Amino Acids

96.32

Weight (kDa)

5.85

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 853 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1054
Acc36I ACCTGC 1 cut(s) 1445
Acc65I GGTACC 1 cut(s) 1928
AccB1I GGYRCC 3 cut(s) 4, 1262, 1928
AccB7I CCANNNNNTGG 2 cut(s) 2173, 2401
AccI GTMKAC 2 cut(s) 338, 2408
AciI CCGC 2 cut(s) 651, 2181
AclWI GGATC 8 cut(s) 934, 953, 1682, 1695, 1732, 1843, 2255, 2272
AcsI RAATTY 9 cut(s) 274, 287, 671, 743, 1225, 1511, 1823, 2016, 2586
AcuI CTGAAG 2 cut(s) 141, 1470
AfaI GTAC 5 cut(s) 478, 762, 1288, 1930, 2319
AfiI CCNNNNNNNGG 5 cut(s) 832, 1363, 1839, 2173, 2401
AflIII ACRYGT 1 cut(s) 2370
AhlI ACTAGT 1 cut(s) 1372
AjnI CCWGG 4 cut(s) 472, 1265, 1282, 2223
AjuI GAANNNNNNNTTGG 2 cut(s) 2222, 2254
Alw21I GWGCWC 4 cut(s) 396, 607, 686, 1533
Alw26I GTCTC 5 cut(s) 638, 730, 1330, 1692, 2490
Alw44I GTGCAC 1 cut(s) 682
AlwI GGATC 8 cut(s) 934, 953, 1682, 1695, 1732, 1843, 2255, 2272
AoxI GGCC 7 cut(s) 594, 968, 1029, 1519, 2196, 2226, 2469
ApaLI GTGCAC 1 cut(s) 682
ApeKI GCWGC 2 cut(s) 1909, 2579
ApoI RAATTY 9 cut(s) 274, 287, 671, 743, 1225, 1511, 1823, 2016, 2586
AseI ATTAAT 1 cut(s) 804
Asp700I GAANNNNTTC 2 cut(s) 1159, 1193
Asp718I GGTACC 1 cut(s) 1928
AspLEI GCGC 1 cut(s) 2353
AspS9I GGNCC 5 cut(s) 613, 938, 1856, 2080, 2164
AsuC2I CCSGG 1 cut(s) 1332
AsuHPI GGTGA 5 cut(s) 130, 1378, 1427, 1679, 2287
AsuII TTCGAA 1 cut(s) 1136
AsuNHI GCTAGC 1 cut(s) 1601
AvaII GGWCC 5 cut(s) 613, 938, 1856, 2080, 2164
BaeGI GKGCMC 1 cut(s) 686
BamHI GGATCC 1 cut(s) 1687
BanI GGYRCC 3 cut(s) 4, 1262, 1928
BanII GRGCYC 1 cut(s) 1533
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 4 cut(s) 396, 607, 686, 1533
BbvI GCAGC 2 cut(s) 1921, 2566
BceAI ACGGC 2 cut(s) 186, 1506
BcgI CGANNNNNNTGC 2 cut(s) 790, 824
BciT130I CCWGG 4 cut(s) 474, 1267, 1284, 2225
BclI TGATCA 1 cut(s) 1405
BcnI CCSGG 1 cut(s) 1332
BcoDI GTCTC 5 cut(s) 638, 730, 1330, 1692, 2490
BcuI ACTAGT 1 cut(s) 1372
BfaI CTAG 9 cut(s) 662, 848, 876, 1337, 1358, 1373, 1602, 1839, 1965
BfmI CTRYAG 3 cut(s) 598, 1150, 2514
BfuAI ACCTGC 1 cut(s) 1445
BglII AGATCT 1 cut(s) 458
BisI GCNGC 3 cut(s) 1910, 2181, 2580
BlpI GCTNAGC 1 cut(s) 197
BlsI GCNGC 3 cut(s) 1911, 2182, 2581
Bme1390I CCNGG 5 cut(s) 474, 1267, 1284, 1332, 2225
Bme18I GGWCC 5 cut(s) 613, 938, 1856, 2080, 2164
BmgT120I GGNCC 5 cut(s) 613, 938, 1856, 2080, 2164
BmrFI CCNGG 5 cut(s) 474, 1267, 1284, 1332, 2225
BmrI ACTGGG 2 cut(s) 944, 2107
BmsI GCATC 4 cut(s) 532, 539, 682, 826
BmtI GCTAGC 1 cut(s) 1605
BmuI ACTGGG 2 cut(s) 944, 2107
BpiI GAAGAC 1 cut(s) 249
BpmI CTGGAG 2 cut(s) 1062, 2423
Bpu1102I GCTNAGC 1 cut(s) 197
Bpu14I TTCGAA 1 cut(s) 1136
BpuEI CTTGAG 3 cut(s) 911, 1228, 2461
BpuMI CCSGG 1 cut(s) 1332
Bsa29I ATCGAT 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 1692
BsaJI CCNNGG 9 cut(s) 235, 730, 767, 987, 1266, 1633, 1704, 2065, 2564
BsaWI WCCGGW 2 cut(s) 45, 1858
BsaXI ACNNNNNCTCC 6 cut(s) 1458, 1488, 1509, 1539, 1845, 1875
Bsc4I CCNNNNNNNGG 5 cut(s) 832, 1363, 1839, 2173, 2401
Bse1I ACTGG 7 cut(s) 29, 939, 1594, 1721, 1801, 2113, 2315
Bse3DI GCAATG 3 cut(s) 110, 139, 1624
BseBI CCWGG 4 cut(s) 474, 1267, 1284, 2225
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 9 cut(s) 235, 730, 767, 987, 1266, 1633, 1704, 2065, 2564
BseGI GGATG 8 cut(s) 697, 841, 1237, 1311, 1399, 1483, 1770, 1932
BseLI CCNNNNNNNGG 5 cut(s) 832, 1363, 1839, 2173, 2401
BseMI GCAATG 3 cut(s) 110, 139, 1624
BseMII CTCAG 5 cut(s) 42, 211, 495, 599, 1497
BseNI ACTGG 7 cut(s) 29, 939, 1594, 1721, 1801, 2113, 2315
BseRI GAGGAG 1 cut(s) 908
BseSI GKGCMC 1 cut(s) 686
BseXI GCAGC 2 cut(s) 1921, 2566
BshFI GGCC 7 cut(s) 596, 970, 1031, 1521, 2198, 2228, 2471
BshNI GGYRCC 3 cut(s) 4, 1262, 1928
BshVI ATCGAT 1 cut(s) 444
BsiHKAI GWGCWC 4 cut(s) 396, 607, 686, 1533
BsiSI CCGG 4 cut(s) 46, 1331, 1833, 1859
BslFI GGGAC 7 cut(s) 202, 874, 1347, 1982, 2093, 2177, 2536
BslI CCNNNNNNNGG 5 cut(s) 832, 1363, 1839, 2173, 2401
BsmAI GTCTC 5 cut(s) 638, 730, 1330, 1692, 2490
BsmFI GGGAC 7 cut(s) 202, 874, 1347, 1982, 2093, 2177, 2536
BsnI GGCC 7 cut(s) 596, 970, 1031, 1521, 2198, 2228, 2471
Bso31I GGTCTC 1 cut(s) 1692
Bsp119I TTCGAA 1 cut(s) 1136
Bsp1286I GDGCHC 4 cut(s) 396, 607, 686, 1533
Bsp1720I GCTNAGC 1 cut(s) 197
Bsp19I CCATGG 2 cut(s) 730, 1704
BspACI CCGC 2 cut(s) 651, 2181
BspANI GGCC 7 cut(s) 596, 970, 1031, 1521, 2198, 2228, 2471
BspCNI CTCAG 5 cut(s) 43, 210, 496, 600, 1498
BspDI ATCGAT 1 cut(s) 444
BspHI TCATGA 1 cut(s) 492
BspMI ACCTGC 1 cut(s) 1445
BspOI GCTAGC 1 cut(s) 1605
BspPI GGATC 8 cut(s) 934, 953, 1682, 1695, 1732, 1843, 2255, 2272
BspQI GCTCTTC 1 cut(s) 1962
BspT104I TTCGAA 1 cut(s) 1136
BspT107I GGYRCC 3 cut(s) 4, 1262, 1928
BspTNI GGTCTC 1 cut(s) 1692
BsrDI GCAATG 3 cut(s) 110, 139, 1624
BsrI ACTGG 7 cut(s) 29, 939, 1594, 1721, 1801, 2113, 2315
BssECI CCNNGG 9 cut(s) 235, 730, 767, 987, 1266, 1633, 1704, 2065, 2564
BssNAI GTATAC 1 cut(s) 2409
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 8 cut(s) 235, 730, 767, 987, 1633, 1704, 2065, 2564
Bst1107I GTATAC 1 cut(s) 2409
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 4 cut(s) 474, 1267, 1284, 2225
Bst4CI ACNGT 4 cut(s) 602, 709, 2381, 2550
Bst6I CTCTTC 3 cut(s) 1654, 1962, 2243
BstBI TTCGAA 1 cut(s) 1136
BstC8I GCNNGC 5 cut(s) 1251, 1533, 1603, 1607, 1946
BstDEI CTNAG 6 cut(s) 51, 197, 308, 504, 608, 1506
BstDSI CCRYGG 2 cut(s) 730, 1704
BstF5I GGATG 8 cut(s) 697, 841, 1237, 1311, 1399, 1483, 1770, 1932
BstHHI GCGC 1 cut(s) 2353
BstMAI GTCTC 5 cut(s) 638, 730, 1330, 1692, 2490
BstMWI GCNNNNNNNGC 4 cut(s) 602, 886, 1155, 1164
BstNI CCWGG 4 cut(s) 474, 1267, 1284, 2225
BstNSI RCATGY 6 cut(s) 1203, 1253, 1609, 1762, 2147, 2374
BstSCI CCNGG 5 cut(s) 472, 1265, 1282, 1330, 2223
BstSFI CTRYAG 3 cut(s) 598, 1150, 2514
BstSLI GKGCMC 1 cut(s) 686
BstV1I GCAGC 2 cut(s) 1921, 2566
BstV2I GAAGAC 1 cut(s) 249
BstX2I RGATCY 4 cut(s) 458, 926, 1687, 1835
BstXI CCANNNNNNTGG 2 cut(s) 2072, 2272
BstYI RGATCY 4 cut(s) 458, 926, 1687, 1835
BstZ17I GTATAC 1 cut(s) 2409
Bsu15I ATCGAT 1 cut(s) 444
BsuRI GGCC 7 cut(s) 596, 970, 1031, 1521, 2198, 2228, 2471
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 2 cut(s) 730, 1704
BtgZI GCGATG 1 cut(s) 532
BtsCI GGATG 8 cut(s) 697, 841, 1237, 1311, 1399, 1483, 1770, 1932
BtsI GCAGTG 1 cut(s) 1135
BtsIMutI CAGTG 6 cut(s) 214, 607, 1135, 2120, 2145, 2386
BveI ACCTGC 1 cut(s) 1445
Cac8I GCNNGC 5 cut(s) 1251, 1533, 1603, 1607, 1946
CciI TCATGA 1 cut(s) 492
CfoI GCGC 1 cut(s) 2353
Cfr13I GGNCC 5 cut(s) 613, 938, 1856, 2080, 2164
ClaI ATCGAT 1 cut(s) 444
CsiI ACCWGGT 2 cut(s) 472, 1282
Csp6I GTAC 5 cut(s) 477, 761, 1287, 1929, 2318
CspCI CAANNNNNGTGG 2 cut(s) 2020, 2055
CviQI GTAC 5 cut(s) 477, 761, 1287, 1929, 2318
DdeI CTNAG 6 cut(s) 51, 197, 308, 504, 608, 1506
DrdI GACNNNNNNGTC 1 cut(s) 1054
DseDI GACNNNNNNGTC 1 cut(s) 1054
Eam1104I CTCTTC 3 cut(s) 1654, 1962, 2243
EarI CTCTTC 3 cut(s) 1654, 1962, 2243
Ecl136II GAGCTC 1 cut(s) 1531
Eco130I CCWWGG 8 cut(s) 235, 730, 767, 987, 1633, 1704, 2065, 2564
Eco147I AGGCCT 1 cut(s) 596
Eco24I GRGCYC 1 cut(s) 1533
Eco31I GGTCTC 1 cut(s) 1692
Eco32I GATATC 1 cut(s) 984
Eco47I GGWCC 5 cut(s) 613, 938, 1856, 2080, 2164
Eco53kI GAGCTC 1 cut(s) 1531
Eco57I CTGAAG 2 cut(s) 141, 1470
EcoICRI GAGCTC 1 cut(s) 1531
EcoO109I RGGNCCY 2 cut(s) 938, 2080
EcoRI GAATTC 2 cut(s) 743, 1511
EcoRII CCWGG 4 cut(s) 472, 1265, 1282, 2223
EcoRV GATATC 1 cut(s) 984
EcoT14I CCWWGG 8 cut(s) 235, 730, 767, 987, 1633, 1704, 2065, 2564
EcoT38I GRGCYC 1 cut(s) 1533
ErhI CCWWGG 8 cut(s) 235, 730, 767, 987, 1633, 1704, 2065, 2564
FalI AAGNNNNNCTT 4 cut(s) 1147, 1179, 1973, 2005
FaqI GGGAC 7 cut(s) 202, 874, 1347, 1982, 2093, 2177, 2536
FauNDI CATATG 1 cut(s) 1734
FbaI TGATCA 1 cut(s) 1405
FblI GTMKAC 2 cut(s) 338, 2408
Fnu4HI GCNGC 3 cut(s) 1910, 2181, 2580
FokI GGATG 8 cut(s) 704, 848, 1244, 1298, 1386, 1490, 1777, 1919
FriOI GRGCYC 1 cut(s) 1533
Fsp4HI GCNGC 3 cut(s) 1910, 2181, 2580
FspBI CTAG 9 cut(s) 662, 848, 876, 1337, 1358, 1373, 1602, 1839, 1965
GlaI GCGC 1 cut(s) 2352
GluI GCNGC 3 cut(s) 1910, 2181, 2580
GsuI CTGGAG 2 cut(s) 1062, 2423
HaeIII GGCC 7 cut(s) 596, 970, 1031, 1521, 2198, 2228, 2471
HapII CCGG 4 cut(s) 46, 1331, 1833, 1859
HhaI GCGC 1 cut(s) 2353
Hin6I GCGC 1 cut(s) 2351
HinP1I GCGC 1 cut(s) 2351
HincII GTYRAC 2 cut(s) 270, 2029
HindII GTYRAC 2 cut(s) 270, 2029
HinfI GANTC 8 cut(s) 251, 844, 862, 1467, 1874, 2097, 2423, 2557
HpaII CCGG 4 cut(s) 46, 1331, 1833, 1859
HphI GGTGA 5 cut(s) 130, 1378, 1427, 1679, 2287
Hpy166II GTNNAC 9 cut(s) 270, 339, 684, 761, 1714, 1918, 2029, 2409, 2546
Hpy8I GTNNAC 9 cut(s) 270, 339, 684, 761, 1714, 1918, 2029, 2409, 2546
HpyAV CCTTC 4 cut(s) 706, 960, 1118, 1136
HpyCH4III ACNGT 4 cut(s) 602, 709, 2381, 2550
HpyF10VI GCNNNNNNNGC 4 cut(s) 602, 886, 1155, 1164
HpyF3I CTNAG 6 cut(s) 51, 197, 308, 504, 608, 1506
HspAI GCGC 1 cut(s) 2351
KflI GGGWCCC 1 cut(s) 2080
KpnI GGTACC 1 cut(s) 1932
Ksp22I TGATCA 1 cut(s) 1405
LguI GCTCTTC 1 cut(s) 1962
LmnI GCTCC 3 cut(s) 399, 2051, 2336
Lsp1109I GCAGC 2 cut(s) 1921, 2566
LweI GCATC 4 cut(s) 532, 539, 682, 826
MabI ACCWGGT 2 cut(s) 472, 1282
MaeI CTAG 9 cut(s) 662, 848, 876, 1337, 1358, 1373, 1602, 1839, 1965
MaeIII GTNAC 5 cut(s) 136, 1045, 1871, 1886, 2518
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 4 cut(s) 458, 926, 1687, 1835
MhlI GDGCHC 4 cut(s) 396, 607, 686, 1533
MlyI GAGTC 4 cut(s) 260, 856, 1476, 1868
MmeI TCCRAC 6 cut(s) 209, 591, 1077, 1985, 2305, 2358
MroXI GAANNNNTTC 2 cut(s) 1159, 1193
MseI TTAA 6 cut(s) 24, 93, 704, 804, 1278, 2448
MslI CAYNNNNRTG 4 cut(s) 584, 1763, 1815, 2270
MspI CCGG 4 cut(s) 46, 1331, 1833, 1859
MspR9I CCNGG 5 cut(s) 474, 1267, 1284, 1332, 2225
MunI CAATTG 1 cut(s) 993
MvaI CCWGG 4 cut(s) 474, 1267, 1284, 2225
MwoI GCNNNNNNNGC 4 cut(s) 602, 886, 1155, 1164
NciI CCSGG 1 cut(s) 1332
NcoI CCATGG 2 cut(s) 730, 1704
NdeI CATATG 1 cut(s) 1734
NheI GCTAGC 1 cut(s) 1601
NmeAIII GCCGAG 1 cut(s) 2307
NmuCI GTSAC 5 cut(s) 136, 1045, 1871, 1886, 2518
NspI RCATGY 6 cut(s) 1203, 1253, 1609, 1762, 2147, 2374
NspV TTCGAA 1 cut(s) 1136
PaeI GCATGC 2 cut(s) 1253, 1609
PagI TCATGA 1 cut(s) 492
PceI AGGCCT 1 cut(s) 596
PciI ACATGT 1 cut(s) 2370
PciSI GCTCTTC 1 cut(s) 1962
PdmI GAANNNNTTC 2 cut(s) 1159, 1193
PfeI GAWTC 4 cut(s) 844, 2097, 2423, 2557
PflMI CCANNNNNTGG 2 cut(s) 2173, 2401
PfoI TCCNGGA 1 cut(s) 1330
PkrI GCNGC 3 cut(s) 1911, 2182, 2581
PleI GAGTC 4 cut(s) 259, 856, 1475, 1868
PpsI GAGTC 4 cut(s) 259, 856, 1475, 1868
PpuMI RGGWCCY 2 cut(s) 938, 2080
PscI ACATGT 1 cut(s) 2370
PshBI ATTAAT 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 1533
Psp5II RGGWCCY 2 cut(s) 938, 2080
Psp6I CCWGG 4 cut(s) 472, 1265, 1282, 2223
PspGI CCWGG 4 cut(s) 472, 1265, 1282, 2223
PspPI GGNCC 5 cut(s) 613, 938, 1856, 2080, 2164
PspPPI RGGWCCY 2 cut(s) 938, 2080
PsrI GAACNNNNNNTAC 2 cut(s) 1144, 1176
PsuI RGATCY 4 cut(s) 458, 926, 1687, 1835
RsaI GTAC 5 cut(s) 478, 762, 1288, 1930, 2319
RsaNI GTAC 5 cut(s) 477, 761, 1287, 1929, 2318
RseI CAYNNNNRTG 4 cut(s) 584, 1763, 1815, 2270
SacI GAGCTC 1 cut(s) 1533
SapI GCTCTTC 1 cut(s) 1962
SaqAI TTAA 6 cut(s) 24, 93, 704, 804, 1278, 2448
SatI GCNGC 3 cut(s) 1910, 2181, 2580
Sau96I GGNCC 5 cut(s) 613, 938, 1856, 2080, 2164
SchI GAGTC 4 cut(s) 260, 856, 1476, 1868
ScrFI CCNGG 5 cut(s) 474, 1267, 1284, 1332, 2225
SduI GDGCHC 4 cut(s) 396, 607, 686, 1533
SexAI ACCWGGT 2 cut(s) 472, 1282
SfaNI GCATC 4 cut(s) 532, 539, 682, 826
SfcI CTRYAG 3 cut(s) 598, 1150, 2514
SfuI TTCGAA 1 cut(s) 1136
SinI GGWCC 5 cut(s) 613, 938, 1856, 2080, 2164
SmiMI CAYNNNNRTG 4 cut(s) 584, 1763, 1815, 2270
SmlI CTYRAG 3 cut(s) 890, 1207, 2476
SmoI CTYRAG 3 cut(s) 890, 1207, 2476
SpeI ACTAGT 1 cut(s) 1372
SphI GCATGC 2 cut(s) 1253, 1609
SseBI AGGCCT 1 cut(s) 596
SsiI CCGC 2 cut(s) 651, 2181
SspI AATATT 1 cut(s) 16
SspMI CTAG 9 cut(s) 662, 848, 876, 1337, 1358, 1373, 1602, 1839, 1965
SstI GAGCTC 1 cut(s) 1533
StuI AGGCCT 1 cut(s) 596
StyD4I CCNGG 5 cut(s) 472, 1265, 1282, 1330, 2223
StyI CCWWGG 8 cut(s) 235, 730, 767, 987, 1633, 1704, 2065, 2564
TaaI ACNGT 4 cut(s) 602, 709, 2381, 2550
TaqI TCGA 6 cut(s) 291, 444, 533, 810, 1136, 2295
TatI WGTACW 1 cut(s) 2317
TauI GCSGC 1 cut(s) 2183
TfiI GAWTC 4 cut(s) 844, 2097, 2423, 2557
Tru1I TTAA 6 cut(s) 24, 93, 704, 804, 1278, 2448
Tru9I TTAA 6 cut(s) 24, 93, 704, 804, 1278, 2448
TscAI CASTG 6 cut(s) 214, 607, 1135, 2120, 2145, 2386
TseFI GTSAC 5 cut(s) 136, 1045, 1871, 1886, 2518
TseI GCWGC 2 cut(s) 1909, 2579
Tsp45I GTSAC 5 cut(s) 136, 1045, 1871, 1886, 2518
TspRI CASTG 6 cut(s) 214, 607, 1135, 2120, 2145, 2386
Van91I CCANNNNNTGG 2 cut(s) 2173, 2401
VneI GTGCAC 1 cut(s) 682
VpaK11BI GGWCC 5 cut(s) 613, 938, 1856, 2080, 2164
VspI ATTAAT 1 cut(s) 804
XapI RAATTY 9 cut(s) 274, 287, 671, 743, 1225, 1511, 1823, 2016, 2586
XceI RCATGY 6 cut(s) 1203, 1253, 1609, 1762, 2147, 2374
XmiI GTMKAC 2 cut(s) 338, 2408
XmnI GAANNNNTTC 2 cut(s) 1159, 1193
XspI CTAG 9 cut(s) 662, 848, 876, 1337, 1358, 1373, 1602, 1839, 1965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.