Rorug02G0014400

acetyltransferase component of pyruvate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002202
Physical Location & Seq
Forward (+)
1219022 .. 1224190
5169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0014400.1

Sequence Viewer

Length: 2838 bp
ATGGCCAATATCTCCTTAGAGGACGTGAAAAAGGAGAACGTCGACCTTGAGAAAATCCCGGTGGAGGAAGTGTTCGAGCAGCTGAAATGCTCGAAGGAGGGGTTGAGCTCAGAGGAGGGCACGAAGAGGATCCAGGTTTTCGGCCCGAATAAGCTCGAGGAAAAGAAAGAGAGCAAGGTTCTCAAGTTCCTCGGGTTCATGTGGAATCCCTTATCATGGGTGATGGAGGCTGCTGCAATCATGGCCATTGTTTTGGCCAATGGAGGGGGCAAGCCTCCGGATTGGCCAGACTTCGTCGGTATTGTGGTGCTTCTGATCATCAACTCAACTATATCATTCATCGAAGAGAACAATGCCGGTAACGCTGCTGCGGCTCTGATGGCTAGTCTTGCCCCCAAGACCAAGGTGTTACGGGATGGAAAATGGGGTGAGCAAGAGGCAGAAATTTTGGTACCAGGGGATGTGATTAGCATCAAGTTGGGAGATATAGTCCCAGCTGATGCTCGTCTCCTGGAGGGCGATCCCTTAAAGATTGACCAAGCTGCGCTCACTGGTGAATCCCTACCGGTCACCAGGCACCCCGGGGATGAGGTCTTCTCTGGATCTACATGCAAGCAGGGCGAGATTGAGGCCGTTGTAATCGCAACTGGAGTCCACACTTTCTTTGGCAAGGCTGCACACTTGGTGGATAGCACCAACCAAGTTGGTCACTTCCAAAAGGTGTTAACATCCATTGGTAACTTCTGCATCTGCTCCATTGGAATTGGAATGGTGATTGAGATACTGGTGATGTACCCCATTCAGCACAGGGCATATAGAGATGGTATTGATAATCTGTTGGTGCTCCTGATCGGAGGTATCCCCATTGCCATGCCAACGGTCTTATCCGTGACCATGGCTATTGGGTCTCACCGCTTGTCACAGCAAGGTGCCATCACCAAGAGGATGACAGCCATTGAAGAAATGGCAGGAATGGATGTCCTTTGCAGTGACAAGACCGGAACCCTCACCCTCAACAAGCTTACCGTCGACAAGAGTCTGATTGAGGTGTTCCCTAAGAACATGGACAAAGACACAGTGGTCTTGCTCTCTGCCAGAGCTTCCAGGGTCGAAAACCAGGACGCAATTGATGCCTCAATTGTTGGGATGCTAAGTGACCCCAAGGAGGCAAGAGCAGGGATCACTGAGGTACATTTCCTGCCCTTCAACCCTGTCGACAAGCGCACAGCAATCACCTACATCGATAGCAATGGCGAGTGGCACAGATGCAGCAAGGGTGCTCCTGAGCAAATAATTGACCTATGTGCCCTTAAGGGAGAAGCGAGGAAGAAGGCCCATGAAGTGATTGACAACTATGCTAACCGTGGTCTTCGTTCATTGGGAGTTGCACGACAGACAGTATCAGAGAAGACCAAGGAAAGTGCTGGTGATGAATGGGAGTTTGTGGGTCTATTGCCTCTCTTTGATCCTCCAAGGCATGACAGTGCTGAGACTATCCGCCGTGCTCTTGAGCTTGGTGTCAATGTTAAGATGATCACTGGTGACCAACTGGCCATTGGAAAAGAGACTGGTCGAAGGCTTGGCATGGGCACCAACATGTATCCTTCATCTACCCTTCTTGGCGAAAGCAAGGACGAGTCCATTGCTTCAATTCCCGTTGATGAGCTCATTGAAAAGGCTGATGGATTTGCTGGAGTCTTCCCTGAGCACAAGTATGAGATTGTTAAGAGACTGCAGGAGAGGAAGCACATCTGTGGTATGACCGGAGATGGTGTGAATGATGCCCCGGCTCTCAAGAGAGCAGACATTGGCATCGCAGTGGATGATGCAACTGATGCAGCCAGGAGTGCCTCGGACATTGTACTGACTGAGCCAGGACTAAGTGTTATTGTGAGCGCCGTCTTGACAAGCAGAGCCATCTTCCAGAGGATGAAGAACTATACCATTTATGCTGTTTCCATCACAATCCGTATTGTGCTGGGATTCATGCTTCTTGCTCTCATATGGAAATATGACTTCCCACCATTCATGGTCTTGGTCATTGCAGTCCTGAACGACGGGACCATCATGACCATCTCTAAGGATAGAGTGAAGCCATCTCCTCTGCCTGACTCCTGGAAACTCAATGAAATCTTCGCCACTGGAGTTGTCCTTGGTTCCTACATGGCTGTCATGACTGTGGTCTTCTTCTGGCTTGCACACAGCACTCTCTTTTTCTCTAAAATATTTGGTGTGAGACCTATTGAGGACCTTCAGCTTAACTCAGCTGTCTACCTCCAAGTGAGCATTCTCAGTCAAGCACTCATCTTTGTGACCCGATCAAGGAGCTGGTCCTTTGTTGAACGCCCTGGTACCATGCTTATGGTTGCCTTTCTTGCAGCGCAGTTGGTGGCTACCCTCATTGCCGTATATTGTGACTGGGGCTTTGCCAAAATCAAGGGTATCGGATGGGGATGGGCAGGCGCTATCTGGGTCTACAGCATTGTCACCTATTTCCCTCTCGATATCTTCAAGTTCCTGATCCGTTATGCATTGAGTGGCCATGCCTGGGATAATGTTGTCCAGAACAAGACTGCCTTCACAACAAAGAATGACTATGGAAAGCCTGAGAGAGAGGCACAGTGGGCATCGGCTCAACGTACACTCCATGGTCTCCAGGCTCCTCCGGAAATGTTCCATGGAAGCCACGGGGATGATCAATCCATTGCTGAGCAGGCCAAGAGGCGAGCAGAAGTTGCTAGGTTGAGGGAGCTCCACACCTTAAAGGGGCACGTCGAATCTGTGGTTAAGCTGAAGGGGCTAGACATCGACACCATCCAACAACACTACACCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

945

Amino Acids

103.69

Weight (kDa)

5.93

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 18 - 81 8.2e-16 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 130 - 229 2.5e-29 P-type ATPase actuator domain
Hydrolase PF00702 325 - 602 8.1e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 388 - 493 9.5e-07 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1079
Acc65I GGTACC 2 cut(s) 451, 2351
AccB1I GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
AccI GTMKAC 5 cut(s) 42, 1029, 1215, 2271, 2475
AccIII TCCGGA 2 cut(s) 277, 2665
AciI CCGC 3 cut(s) 371, 913, 1498
AclWI GGATC 7 cut(s) 124, 137, 515, 610, 1187, 1460, 2515
AcoI YGGCCR 6 cut(s) 3, 243, 255, 284, 1551, 2539
AcsI RAATTY 1 cut(s) 444
AcuI CTGAAG 2 cut(s) 2237, 2813
AdeI CACNNNGTG 1 cut(s) 685
AfaI GTAC 6 cut(s) 453, 794, 1191, 1863, 2353, 2641
AfiI CCNNNNNNNGG 7 cut(s) 64, 216, 264, 1165, 2322, 2548, 2766
AflII CTTAAG 1 cut(s) 1310
AflIII ACRYGT 1 cut(s) 1596
AgeI ACCGGT 1 cut(s) 565
AgsI TTSAA 6 cut(s) 959, 1207, 1650, 1673, 2342, 2512
AjiI CACGTC 2 cut(s) 25, 2773
AjuI GAANNNNNNNTTGG 2 cut(s) 2271, 2303
AleI CACNNNNGTG 1 cut(s) 1752
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
Alw21I GWGCWC 7 cut(s) 110, 846, 1282, 1507, 1668, 1710, 2754
Alw26I GTCTC 7 cut(s) 512, 912, 1484, 1559, 1723, 2230, 2657
AlwI GGATC 7 cut(s) 124, 137, 515, 610, 1187, 1460, 2515
Ama87I CYCGRG 3 cut(s) 155, 191, 581
Aor13HI TCCGGA 2 cut(s) 277, 2665
ApoI RAATTY 1 cut(s) 444
ArsI GACNNNNNNTTYG 2 cut(s) 2408, 2440
AsiGI ACCGGT 1 cut(s) 565
Asp718I GGTACC 2 cut(s) 451, 2351
AspLEI GCGC 5 cut(s) 547, 1224, 1898, 2383, 2465
AspS9I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
AsuC2I CCSGG 4 cut(s) 59, 582, 583, 1787
AvaI CYCGRG 3 cut(s) 155, 191, 581
AvaII GGWCC 3 cut(s) 2061, 2248, 2331
BaeGI GKGCMC 4 cut(s) 122, 1309, 1592, 2772
BalI TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
BamHI GGATCC 1 cut(s) 129
BanI GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
BanII GRGCYC 3 cut(s) 110, 1668, 2754
BbsI GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
Bbv12I GWGCWC 7 cut(s) 110, 846, 1282, 1507, 1668, 1710, 2754
BceAI ACGGC 4 cut(s) 617, 1485, 1883, 2390
BcgI CGANNNNNNTGC 2 cut(s) 1625, 1659
BciVI GTATCC 2 cut(s) 869, 1611
BclI TGATCA 3 cut(s) 315, 1533, 2695
BcnI CCSGG 4 cut(s) 59, 582, 583, 1787
BcoDI GTCTC 7 cut(s) 512, 912, 1484, 1559, 1723, 2230, 2657
BfaI CTAG 4 cut(s) 384, 2739, 2801, 2836
BfmI CTRYAG 2 cut(s) 1733, 2476
BfoI RGCGCY 2 cut(s) 1899, 2466
BfrI CTTAAG 1 cut(s) 1310
BfuI GTATCC 2 cut(s) 869, 1611
BlpI GCTNAGC 1 cut(s) 2709
Bme18I GGWCC 3 cut(s) 2061, 2248, 2331
BmeT110I CYCGRG 3 cut(s) 155, 191, 581
BmgBI CACGTC 2 cut(s) 25, 2773
BmgT120I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
BmrI ACTGGG 1 cut(s) 2428
BmuI ACTGGG 1 cut(s) 2428
BoxI GACNNNNGTC 2 cut(s) 1035, 2180
BpiI GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 5 cut(s) 533, 669, 1713, 2163, 2639
Bpu10I CCTNAGC 2 cut(s) 1284, 1704
Bpu1102I GCTNAGC 1 cut(s) 2709
BpuEI CTTGAG 4 cut(s) 68, 167, 1529, 1778
BpuMI CCSGG 4 cut(s) 59, 582, 583, 1787
Bsa29I ATCGAT 1 cut(s) 1242
BsaBI GATNNNNATC 1 cut(s) 470
BsaI GGTCTC 3 cut(s) 912, 2230, 2657
BsaWI WCCGGW 5 cut(s) 277, 565, 998, 1763, 2665
BsaXI ACNNNNNCTCC 8 cut(s) 56, 86, 474, 504, 1758, 1788, 2628, 2658
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 216, 264, 1165, 2322, 2548, 2766
Bse118I RCCGGY 2 cut(s) 356, 565
Bse1I ACTGG 8 cut(s) 556, 652, 789, 1543, 1554, 1573, 2146, 2423
Bse3DI GCAATG 6 cut(s) 864, 1255, 1641, 2040, 2400, 2703
Bse8I GATNNNNATC 1 cut(s) 470
BseAI TCCGGA 2 cut(s) 277, 2665
BseCI ATCGAT 1 cut(s) 1242
BseJI GATNNNNATC 1 cut(s) 470
BseLI CCNNNNNNNGG 7 cut(s) 64, 216, 264, 1165, 2322, 2548, 2766
BseMI GCAATG 6 cut(s) 864, 1255, 1641, 2040, 2400, 2703
BseNI ACTGG 8 cut(s) 556, 652, 789, 1543, 1554, 1573, 2146, 2423
BseRI GAGGAG 3 cut(s) 128, 2091, 2652
BseSI GKGCMC 4 cut(s) 122, 1309, 1592, 2772
BseYI CCCAGC 2 cut(s) 493, 1978
BsgI GTGCAG 1 cut(s) 660
BshNI GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
BshTI ACCGGT 1 cut(s) 565
BshVI ATCGAT 1 cut(s) 1242
BsiHKAI GWGCWC 7 cut(s) 110, 846, 1282, 1507, 1668, 1710, 2754
BsiHKCI CYCGRG 3 cut(s) 155, 191, 581
BsiSI CCGG 9 cut(s) 59, 278, 357, 566, 582, 999, 1764, 1787, 2666
BslFI GGGAC 2 cut(s) 476, 2074
BslI CCNNNNNNNGG 7 cut(s) 64, 216, 264, 1165, 2322, 2548, 2766
BsmAI GTCTC 7 cut(s) 512, 912, 1484, 1559, 1723, 2230, 2657
BsmBI CGTCTC 1 cut(s) 512
BsmFI GGGAC 2 cut(s) 476, 2074
BsmI GAATGC 1 cut(s) 2286
Bso31I GGTCTC 3 cut(s) 912, 2230, 2657
BsoBI CYCGRG 3 cut(s) 155, 191, 581
Bsp13I TCCGGA 2 cut(s) 277, 2665
Bsp1720I GCTNAGC 1 cut(s) 2709
Bsp19I CCATGG 3 cut(s) 894, 2647, 2677
BspACI CCGC 3 cut(s) 371, 913, 1498
BspDI ATCGAT 1 cut(s) 1242
BspEI TCCGGA 2 cut(s) 277, 2665
BspHI TCATGA 2 cut(s) 2067, 2172
BspMAI CTGCAG 1 cut(s) 1737
BspPI GGATC 7 cut(s) 124, 137, 515, 610, 1187, 1460, 2515
BspT107I GGYRCC 5 cut(s) 451, 576, 929, 1589, 2351
BspTI CTTAAG 1 cut(s) 1310
BspTNI GGTCTC 3 cut(s) 912, 2230, 2657
BsrDI GCAATG 6 cut(s) 864, 1255, 1641, 2040, 2400, 2703
BsrFI RCCGGY 2 cut(s) 356, 565
BsrI ACTGG 8 cut(s) 556, 652, 789, 1543, 1554, 1573, 2146, 2423
BssAI RCCGGY 2 cut(s) 356, 565
BssT1I CCWWGG 8 cut(s) 402, 894, 1161, 1413, 1472, 2152, 2647, 2677
Bst4CI ACNGT 9 cut(s) 880, 1027, 1078, 1364, 1399, 1484, 2179, 2622, 2833
Bst6I CTCTTC 2 cut(s) 119, 339
BstAFI CTTAAG 1 cut(s) 1310
BstAPI GCANNNNNTGC 3 cut(s) 1130, 1835, 2735
BstC8I GCNNGC 6 cut(s) 272, 614, 2196, 2461, 2715, 2727
BstDSI CCRYGG 5 cut(s) 894, 1363, 2647, 2677, 2686
BstEII GGTNACC 2 cut(s) 568, 1541
BstH2I RGCGCY 2 cut(s) 1899, 2466
BstHHI GCGC 5 cut(s) 547, 1224, 1898, 2383, 2465
BstMAI GTCTC 7 cut(s) 512, 912, 1484, 1559, 1723, 2230, 2657
BstNSI RCATGY 2 cut(s) 612, 1600
BstPAI GACNNNNGTC 2 cut(s) 1035, 2180
BstPI GGTNACC 2 cut(s) 568, 1541
BstSFI CTRYAG 2 cut(s) 1733, 2476
BstSLI GKGCMC 4 cut(s) 122, 1309, 1592, 2772
BstV2I GAAGAC 5 cut(s) 586, 1361, 1415, 1690, 2176
BstX2I RGATCY 2 cut(s) 129, 602
BstXI CCANNNNNNTGG 2 cut(s) 253, 2362
BstYI RGATCY 2 cut(s) 129, 602
Bsu15I ATCGAT 1 cut(s) 1242
BsuI GTATCC 2 cut(s) 869, 1611
BsuTUI ATCGAT 1 cut(s) 1242
BtgI CCRYGG 5 cut(s) 894, 1363, 2647, 2677, 2686
BtgZI GCGATG 1 cut(s) 1798
BtrI CACGTC 2 cut(s) 25, 2773
BtsI GCAGTG 2 cut(s) 994, 1824
BtsIMutI CAGTG 9 cut(s) 549, 994, 1083, 1182, 1489, 1536, 1824, 2139, 2627
Cac8I GCNNGC 6 cut(s) 272, 614, 2196, 2461, 2715, 2727
CciI TCATGA 2 cut(s) 2067, 2172
CfoI GCGC 5 cut(s) 547, 1224, 1898, 2383, 2465
Cfr10I RCCGGY 2 cut(s) 356, 565
Cfr13I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
Cfr9I CCCGGG 1 cut(s) 581
ClaI ATCGAT 1 cut(s) 1242
CseI GACGC 1 cut(s) 1130
Csp6I GTAC 6 cut(s) 452, 793, 1190, 1862, 2352, 2640
CspAI ACCGGT 1 cut(s) 565
CspCI CAANNNNNGTGG 2 cut(s) 2128, 2163
CviQI GTAC 6 cut(s) 452, 793, 1190, 1862, 2352, 2640
DraIII CACNNNGTG 1 cut(s) 685
DrdI GACNNNNNNGTC 1 cut(s) 1079
DseDI GACNNNNNNGTC 1 cut(s) 1079
EaeI YGGCCR 6 cut(s) 3, 243, 255, 284, 1551, 2539
Eam1104I CTCTTC 2 cut(s) 119, 339
EarI CTCTTC 2 cut(s) 119, 339
EciI GGCGGA 1 cut(s) 1487
Ecl136II GAGCTC 3 cut(s) 108, 1666, 2752
Eco130I CCWWGG 8 cut(s) 402, 894, 1161, 1413, 1472, 2152, 2647, 2677
Eco24I GRGCYC 3 cut(s) 110, 1668, 2754
Eco31I GGTCTC 3 cut(s) 912, 2230, 2657
Eco32I GATATC 1 cut(s) 2506
Eco47I GGWCC 3 cut(s) 2061, 2248, 2331
Eco53kI GAGCTC 3 cut(s) 108, 1666, 2752
Eco57I CTGAAG 2 cut(s) 2237, 2813
Eco88I CYCGRG 3 cut(s) 155, 191, 581
Eco91I GGTNACC 2 cut(s) 568, 1541
EcoICRI GAGCTC 3 cut(s) 108, 1666, 2752
EcoO109I RGGNCCY 1 cut(s) 2248
EcoO65I GGTNACC 2 cut(s) 568, 1541
EcoRV GATATC 1 cut(s) 2506
EcoT14I CCWWGG 8 cut(s) 402, 894, 1161, 1413, 1472, 2152, 2647, 2677
EcoT22I ATGCAT 1 cut(s) 2533
EcoT38I GRGCYC 3 cut(s) 110, 1668, 2754
ErhI CCWWGG 8 cut(s) 402, 894, 1161, 1413, 1472, 2152, 2647, 2677
Esp3I CGTCTC 1 cut(s) 512
FalI AAGNNNNNCTT 2 cut(s) 2561, 2593
FaqI GGGAC 2 cut(s) 476, 2074
FauNDI CATATG 1 cut(s) 2003
FbaI TGATCA 3 cut(s) 315, 1533, 2695
FblI GTMKAC 5 cut(s) 42, 1029, 1215, 2271, 2475
FriOI GRGCYC 3 cut(s) 110, 1668, 2754
FspBI CTAG 4 cut(s) 384, 2739, 2801, 2836
GlaI GCGC 5 cut(s) 546, 1223, 1897, 2382, 2464
GsaI CCCAGC 2 cut(s) 497, 1982
GsuI CTGGAG 5 cut(s) 533, 669, 1713, 2163, 2639
HaeII RGCGCY 2 cut(s) 1899, 2466
HapII CCGG 9 cut(s) 59, 278, 357, 566, 582, 999, 1764, 1787, 2666
HgaI GACGC 1 cut(s) 1130
HhaI GCGC 5 cut(s) 547, 1224, 1898, 2383, 2465
Hin6I GCGC 5 cut(s) 545, 1222, 1896, 2381, 2463
HinP1I GCGC 5 cut(s) 545, 1222, 1896, 2381, 2463
HincII GTYRAC 4 cut(s) 43, 726, 1030, 1216
HindII GTYRAC 4 cut(s) 43, 726, 1030, 1216
HindIII AAGCTT 1 cut(s) 1019
HinfI GANTC 9 cut(s) 205, 557, 651, 1036, 1637, 1695, 1983, 2111, 2777
HpaI GTTAAC 1 cut(s) 726
HpaII CCGG 9 cut(s) 59, 278, 357, 566, 582, 999, 1764, 1787, 2666
Hpy166II GTNNAC 8 cut(s) 43, 655, 726, 1030, 1216, 2272, 2476, 2642
Hpy188I TCNGA 8 cut(s) 112, 315, 378, 854, 1041, 1405, 1855, 2447
Hpy8I GTNNAC 8 cut(s) 43, 655, 726, 1030, 1216, 2272, 2476, 2642
Hpy99I CGWCG 5 cut(s) 44, 299, 1031, 2060, 2777
HpyAV CCTTC 9 cut(s) 88, 1213, 1324, 1569, 1614, 1625, 2261, 2587, 2788
HpyCH4III ACNGT 9 cut(s) 880, 1027, 1078, 1364, 1399, 1484, 2179, 2622, 2833
HpyCH4IV ACGT 4 cut(s) 24, 39, 2638, 2772
HpySE526I ACGT 4 cut(s) 24, 39, 2638, 2772
HspAI GCGC 5 cut(s) 545, 1222, 1896, 2381, 2463
Kpn2I TCCGGA 2 cut(s) 277, 2665
KpnI GGTACC 2 cut(s) 455, 2355
Ksp22I TGATCA 3 cut(s) 315, 1533, 2695
KspAI GTTAAC 1 cut(s) 726
LmnI GCTCC 7 cut(s) 758, 849, 1285, 2325, 2665, 2749, 2757
MaeI CTAG 4 cut(s) 384, 2739, 2801, 2836
MaeII ACGT 4 cut(s) 24, 39, 2638, 2772
MfeI CAATTG 2 cut(s) 1125, 1137
MflI RGATCY 2 cut(s) 129, 602
MlsI TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
MluCI AATT 6 cut(s) 444, 762, 1125, 1137, 1293, 1650
MluNI TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
MlyI GAGTC 5 cut(s) 660, 1045, 1646, 1704, 2105
Mox20I TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
Mph1103I ATGCAT 1 cut(s) 2533
MroI TCCGGA 2 cut(s) 277, 2665
MscI TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
MseI TTAA 8 cut(s) 527, 725, 1311, 1527, 1725, 2259, 2762, 2787
Msp20I TGGCCA 6 cut(s) 5, 245, 257, 286, 1553, 2541
MspA1I CMGCKG 3 cut(s) 82, 497, 2267
MspCI CTTAAG 1 cut(s) 1310
MspI CCGG 9 cut(s) 59, 278, 357, 566, 582, 999, 1764, 1787, 2666
MunI CAATTG 2 cut(s) 1125, 1137
Mva1269I GAATGC 1 cut(s) 2286
NciI CCSGG 4 cut(s) 59, 582, 583, 1787
NcoI CCATGG 3 cut(s) 894, 2647, 2677
NdeI CATATG 1 cut(s) 2003
NsiI ATGCAT 1 cut(s) 2533
NspI RCATGY 2 cut(s) 612, 1600
OliI CACNNNNGTG 1 cut(s) 1752
PaeR7I CTCGAG 1 cut(s) 155
PagI TCATGA 2 cut(s) 2067, 2172
PciI ACATGT 1 cut(s) 1596
PctI GAATGC 1 cut(s) 2286
PfeI GAWTC 4 cut(s) 205, 557, 1983, 2777
PflFI GACNNNGTC 1 cut(s) 293
PfoI TCCNGGA 2 cut(s) 510, 2114
PinAI ACCGGT 1 cut(s) 565
PleI GAGTC 5 cut(s) 659, 1044, 1645, 1703, 2105
PpsI GAGTC 5 cut(s) 659, 1044, 1645, 1703, 2105
PpuMI RGGWCCY 1 cut(s) 2248
PscI ACATGT 1 cut(s) 1596
PshAI GACNNNNGTC 2 cut(s) 1035, 2180
Psp124BI GAGCTC 3 cut(s) 110, 1668, 2754
Psp5II RGGWCCY 1 cut(s) 2248
PspEI GGTNACC 2 cut(s) 568, 1541
PspFI CCCAGC 2 cut(s) 493, 1978
PspPI GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
PspPPI RGGWCCY 1 cut(s) 2248
PspXI VCTCGAGB 1 cut(s) 155
PstI CTGCAG 1 cut(s) 1737
PsuI RGATCY 2 cut(s) 129, 602
PsyI GACNNNGTC 1 cut(s) 293
PvuII CAGCTG 3 cut(s) 82, 497, 2267
RsaI GTAC 6 cut(s) 453, 794, 1191, 1863, 2353, 2641
RsaNI GTAC 6 cut(s) 452, 793, 1190, 1862, 2352, 2640
SacI GAGCTC 3 cut(s) 110, 1668, 2754
SalI GTCGAC 3 cut(s) 41, 1028, 1214
SaqAI TTAA 8 cut(s) 527, 725, 1311, 1527, 1725, 2259, 2762, 2787
Sau96I GGNCC 5 cut(s) 143, 1333, 2061, 2248, 2331
SchI GAGTC 5 cut(s) 660, 1045, 1646, 1704, 2105
SfcI CTRYAG 2 cut(s) 1733, 2476
Sfr274I CTCGAG 1 cut(s) 155
SinI GGWCC 3 cut(s) 2061, 2248, 2331
SlaI CTCGAG 1 cut(s) 155
SmaI CCCGGG 1 cut(s) 583
SmlI CTYRAG 6 cut(s) 47, 155, 182, 1310, 1508, 1793
SmoI CTYRAG 6 cut(s) 47, 155, 182, 1310, 1508, 1793
Sse9I AATT 6 cut(s) 444, 762, 1125, 1137, 1293, 1650
SsiI CCGC 3 cut(s) 371, 913, 1498
SspI AATATT 1 cut(s) 2226
SspMI CTAG 4 cut(s) 384, 2739, 2801, 2836
SstI GAGCTC 3 cut(s) 110, 1668, 2754
StyI CCWWGG 8 cut(s) 402, 894, 1161, 1413, 1472, 2152, 2647, 2677
TaaI ACNGT 9 cut(s) 880, 1027, 1078, 1364, 1399, 1484, 2179, 2622, 2833
TaiI ACGT 4 cut(s) 27, 42, 2641, 2775
TasI AATT 6 cut(s) 444, 762, 1125, 1137, 1293, 1650
TatI WGTACW 1 cut(s) 1861
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 4 cut(s) 205, 557, 1983, 2777
Tru1I TTAA 8 cut(s) 527, 725, 1311, 1527, 1725, 2259, 2762, 2787
Tru9I TTAA 8 cut(s) 527, 725, 1311, 1527, 1725, 2259, 2762, 2787
TscAI CASTG 9 cut(s) 556, 994, 1083, 1189, 1489, 1543, 1824, 2146, 2627
TspGWI ACGGA 3 cut(s) 877, 1958, 2513
TspMI CCCGGG 1 cut(s) 581
TspRI CASTG 9 cut(s) 556, 994, 1083, 1189, 1489, 1543, 1824, 2146, 2627
Tth111I GACNNNGTC 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 1310
VpaK11BI GGWCC 3 cut(s) 2061, 2248, 2331
XapI RAATTY 1 cut(s) 444
XceI RCATGY 2 cut(s) 612, 1600
XcmI CCANNNNNNNNNTGG 3 cut(s) 662, 961, 1553
XhoI CTCGAG 1 cut(s) 155
XmaI CCCGGG 1 cut(s) 581
XmiI GTMKAC 5 cut(s) 42, 1029, 1215, 2271, 2475
XspI CTAG 4 cut(s) 384, 2739, 2801, 2836
Zsp2I ATGCAT 1 cut(s) 2533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.