Rh3AG280600

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002202
Physical Location & Seq
Reverse (-)
32725084 .. 32728928
3845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG280600.1

Sequence Viewer

Length: 2598 bp
ATGGCACCTCCTAATGATCCTATTAGTCCAGTTTTCAATGCGTTCCGGTCCGAGAGTTCTGGAAAATACTTTGACGTCTCTAATGGAAAGCTTAGTGTCAAAGGGATTCCATTGCTCTCTGAAGTTCCAAGCAATGTAACTTTCAGTCACTTCCACTCACCCTGCCAATCTTCTGATGTTCCACTTCCTCTGCTCCAACGGGTTCGTGCCTCGTCCCACAAGGGTGCTTTCCTTGGATTCAACAAGGAGGTTCCCTCAGATAGGCTGCTCAACTCCTTGGGGAAACTCAGTAACAGGGATTTCCTTAGTATCTTTAGGTTCAAGACATGGTGGTCTACCATGTGGGTGGGAAACTCTGGTTCAAACTTGCAAATGGAAACCCAATGGGTGCTCTTAGATGTCCCGGAGATCAAGTCATATGTTATAATCATACCCATCATTGAAGACAGTTTCAGGTCTGCTCTTCATCCTGGCAGTGATGACCATGTCATGATCTGTGCCGAGAGTGGTTCTAGTCAAGTGAAAGCGTCGAATTTCAATGCCATTGCTTATGTTCATGCTTCTGAAAATCCCTACAACTTGATGAAAGAAGCCTATAGTGCTGTTAGAGTCCATCTCAATAGTTTTAGGCTCTTGGAAGAGAAGACGGTCCCAAATCTAGTTAACAAATTTGGTTGGTGCACTTGGGATGCATTCTACTTAACTGTAGAACCTGTTGGTGTCTGGCATGGTGTGAAAGAATTTGTTGAAGGCGGTGTGTCCCCGCGGTTTCTCATAATTGATGATGGTTGGCAAAGCATCAACATGGATGATGAGGACCTGAAGGAGGACACAAAAAATCTAGTTCTTGGTGGGACTCAAATGACTGCTAGGCTTCACAGTTTTGAGGAGTGCAAAAAGTTCAGGAACTACAGAGGTGGCTCTATGTTGGGTCCTGATGCTCCTTCTTTTGATCCCAAAAAGCCAAAACTGCTGATTGCCAAGGCAATTGAAATAGAGCATGCTGAGAAGGAACACGACAAGGCCCTTCATTCTGGAGTCACTGAGTTGTATGTGTTCGAAAAAAAACTTCAGAAACTGAAGACAGAGTTGAATGATATCATTGGTGAAGGAGAGAGCAGTGCTTCTAATGAATGCCGTGGAAGCAGTTCTGGCAGTGGCAAAAACTATGGAATGAAGGCCTTCACAGGTGACTTGAGAACCAAGTTTAAAGGTTTGGATGACATATATGTATGGCATGCTCTTTGTGGTGCGTGGGGTGGTGTTAGGCCAGGTTCAACTCATCTCAGTTCAAAGATCATTCCTTGCAAAGTCTCACCTGGACTAGATGGGACGATGAATGATCTGGCTGTGGTGAAAATCATTGAGGGCGGGATTGGGCTTGTTCATCCTGATCAAGCTGATAGTTTCTATGACTCCATGCACTCTTACCTTTCTGAAGTTGGTATCACTGGAGTCAAAGTGGATGTAATTCATACACTTGAATATGTATCTGAGGAATACGGAGGCCGTGTGGAGCTTGCAAAGGCTTATTACAAGGGGCTGACTCATTCTCTTCAGAAGAACTTCAATGGGACTGGACTTATTGCTAGCATGCAGCAATGCAATGACTTCTTCTTTCTTGGCACAAAGCAAATCTCTATTGGAAGAGTCGGTGACGATTTTTGGTTTCAGGATCCGAATGGAGATCCAATGGGAGTCTACTGGTTACAAGGAGTTCATATGATCCACTGCGCTTACAACAGCATGTGGATGGGCCAGATGATACAGCCTGATTGGGACATGTTTCAATCAGACCATTTATGTGCCAAATTTCATGCAGGATCAAGAGCAATCTGTGGGGGTCCGGTTTATGTAAGTGACTCAGTTTCAGGGCATGATTTCGATCTCATTAAGAAGCTTGTTTACCCGGATGGTACAATTCCCAAGTGCCAGCATTTCGCTCTCCCAACCAGGGACTGCCTCTTCAAAAACCCTTTATTTGATGATAAGACTGTCCTGAAAATTTGGAACTTTAACAAGTATGGCGGTGTCATTGGAGCTTTCAATTGTCAAGGGGCTGGTTGGGACCCCAAGGAACAGAGGATCAAAGGCCACTCAGAGTGCTACAAGCCAATCTCTTGTTCATTACATGTTCGTGAGATTGAATGGGACCAAAAGTCTGAAGCAGCCCTTTTGGGCGAGGCTGAAGAGTACGTTGTCTACCTCAGCGAGGTTGATGAGCTCCGCCTGATGACCCCAAAGTCCGATGCCATTAAAATAGTCATCCAGCCATCTTCGTTTGAGATTTTGAGCTTTTTGCCGGTTAGAAAGCTAGGCCACAGCATTAAGTTCGCGCCAATTGGACTTACAAACATGTTCAACTGTGGAGGCACACTACAAGAGTTGGAGTACAAAAGTACCGCAGTCGAGTTTAGTGCCATGATCAAAGTTAAAGGTGGAGGCAAGTTCCTGGCCTACTCAAGTGAGTCACCAAAGAAATGTTGTTTGAATGGTGCTGAGGTAGCTTTTGAGTGGTCTGCTGATGGCAAATTGAACTTGAGTGTTCCTTGGGTTGAACAAGCTGCTGGCATTTCTGATGTGCTTGTTGCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

865

Amino Acids

96.04

Weight (kDa)

5.95

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 848 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AasI GACNNNNNNGTC 2 cut(s) 331, 2242
AatII GACGTC 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 3 cut(s) 335, 1701, 2202
AccII CGCG 2 cut(s) 766, 2336
AciI CCGC 7 cut(s) 753, 764, 766, 1371, 2028, 2227, 2403
AclWI GGATC 8 cut(s) 11, 947, 1670, 1682, 1683, 1720, 1831, 2093
AcsI RAATTY 5 cut(s) 532, 668, 740, 1811, 2004
AcuI CTGAAG 8 cut(s) 141, 842, 1055, 1100, 1458, 1541, 2184, 2208
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 4 cut(s) 1918, 2195, 2393, 2401
AfiI CCNNNNNNNGG 4 cut(s) 261, 826, 1954, 2212
AflIII ACRYGT 3 cut(s) 1782, 2131, 2355
AhdI GACNNNNNGTC 1 cut(s) 2158
AjnI CCWGG 5 cut(s) 469, 1270, 1318, 1952, 2451
AjuI GAANNNNNNNTTGG 2 cut(s) 2107, 2139
AleI CACNNNNGTG 1 cut(s) 222
Alw21I GWGCWC 3 cut(s) 393, 683, 2226
Alw26I GTCTC 2 cut(s) 82, 1318
Alw44I GTGCAC 1 cut(s) 679
AlwI GGATC 8 cut(s) 11, 947, 1670, 1682, 1683, 1720, 1831, 2093
AlwNI CAGNNNCTG 2 cut(s) 1078, 1959
AoxI GGCC 8 cut(s) 1023, 1179, 1268, 1507, 1756, 2092, 2317, 2454
ApaLI GTGCAC 1 cut(s) 679
ApeKI GCWGC 4 cut(s) 265, 1597, 2168, 2564
ApoI RAATTY 5 cut(s) 532, 668, 740, 1811, 2004
Asp700I GAANNNNTTC 3 cut(s) 1147, 1181, 1565
AspLEI GCGC 2 cut(s) 1736, 2338
AspS9I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
AsuC2I CCSGG 2 cut(s) 404, 1910
AsuHPI GGTGA 7 cut(s) 150, 1118, 1202, 1308, 1366, 1667, 2463
AsuII TTCGAA 1 cut(s) 1059
AsuNHI GCTAGC 1 cut(s) 1589
AvaII GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
BaeGI GKGCMC 1 cut(s) 683
BaeI ACNNNNGTAYC 2 cut(s) 2383, 2416
BamHI GGATCC 1 cut(s) 1675
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 2226
BarI GAAGNNNNNNTAC 2 cut(s) 1889, 1921
BbsI GAAGAC 3 cut(s) 450, 650, 1088
Bbv12I GWGCWC 3 cut(s) 393, 683, 2226
BbvCI CCTCAGC 2 cut(s) 2207, 2499
BbvI GCAGC 4 cut(s) 252, 1609, 2180, 2551
BccI CCATC 8 cut(s) 443, 621, 779, 1322, 1747, 1907, 2281, 2519
BceAI ACGGC 2 cut(s) 1122, 1494
BcgI CGANNNNNNTGC 4 cut(s) 2314, 2348, 2399, 2433
BciT130I CCWGG 5 cut(s) 471, 1272, 1320, 1954, 2453
BclI TGATCA 2 cut(s) 1393, 2424
BcnI CCSGG 2 cut(s) 404, 1910
BcoDI GTCTC 2 cut(s) 82, 1318
BfaI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
BfmI CTRYAG 3 cut(s) 595, 705, 910
BisI GCNGC 4 cut(s) 266, 1598, 2169, 2565
BlsI GCNGC 4 cut(s) 267, 1599, 2170, 2566
Bme1390I CCNGG 7 cut(s) 404, 471, 1272, 1320, 1910, 1954, 2453
Bme18I GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
BmeRI GACNNNNNGTC 1 cut(s) 2158
BmgT120I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
BmiI GGNNCC 9 cut(s) 6, 252, 651, 933, 1677, 1845, 2069, 2070, 2153
BmrFI CCNGG 7 cut(s) 404, 471, 1272, 1320, 1910, 1954, 2453
BmsI GCATC 4 cut(s) 679, 807, 928, 2239
BmtI GCTAGC 1 cut(s) 1593
BpiI GAAGAC 3 cut(s) 450, 650, 1088
BplI GAGNNNNNCTC 4 cut(s) 239, 271, 600, 632
BpmI CTGGAG 2 cut(s) 1056, 1473
Bpu10I CCTNAGC 2 cut(s) 2207, 2499
Bpu14I TTCGAA 1 cut(s) 1059
BpuEI CTTGAG 3 cut(s) 1216, 2446, 2560
BpuMI CCSGG 2 cut(s) 404, 1910
BsaBI GATNNNNATC 1 cut(s) 1884
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 8 cut(s) 232, 276, 764, 981, 1138, 1953, 2073, 2549
BsaWI WCCGGW 2 cut(s) 45, 1846
BsaXI ACNNNNNCTCC 6 cut(s) 398, 428, 1446, 1476, 1497, 1527
Bsc4I CCNNNNNNNGG 4 cut(s) 261, 826, 1954, 2212
Bse118I RCCGGY 1 cut(s) 2302
Bse1I ACTGG 4 cut(s) 29, 1456, 1582, 1709
Bse3DI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
Bse8I GATNNNNATC 1 cut(s) 1884
BseBI CCWGG 5 cut(s) 471, 1272, 1320, 1954, 2453
BseDI CCNNGG 8 cut(s) 232, 276, 764, 981, 1138, 1953, 2073, 2549
BseGI GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BseJI GATNNNNATC 1 cut(s) 1884
BseLI CCNNNNNNNGG 4 cut(s) 261, 826, 1954, 2212
BseMI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
BseNI ACTGG 4 cut(s) 29, 1456, 1582, 1709
BseRI GAGGAG 1 cut(s) 902
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 4 cut(s) 252, 1609, 2180, 2551
Bsh1236I CGCG 2 cut(s) 766, 2336
BshFI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 3 cut(s) 393, 683, 2226
BsiSI CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
BslI CCNNNNNNNGG 4 cut(s) 261, 826, 1954, 2212
BsmAI GTCTC 2 cut(s) 82, 1318
BsmBI CGTCTC 1 cut(s) 82
BsmI GAATGC 2 cut(s) 692, 1139
BsnI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
Bsp119I TTCGAA 1 cut(s) 1059
Bsp1286I GDGCHC 3 cut(s) 393, 683, 2226
BspACI CCGC 7 cut(s) 753, 764, 766, 1371, 2028, 2227, 2403
BspANI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BspFNI CGCG 2 cut(s) 766, 2336
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 9 cut(s) 6, 252, 651, 933, 1677, 1845, 2069, 2070, 2153
BspOI GCTAGC 1 cut(s) 1593
BspPI GGATC 8 cut(s) 11, 947, 1670, 1682, 1683, 1720, 1831, 2093
BspQI GCTCTTC 1 cut(s) 468
BspT104I TTCGAA 1 cut(s) 1059
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
BsrFI RCCGGY 1 cut(s) 2302
BsrI ACTGG 4 cut(s) 29, 1456, 1582, 1709
BssAI RCCGGY 1 cut(s) 2302
BssECI CCNNGG 8 cut(s) 232, 276, 764, 981, 1138, 1953, 2073, 2549
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
Bst2UI CCWGG 5 cut(s) 471, 1272, 1320, 1954, 2453
Bst4CI ACNGT 6 cut(s) 449, 649, 706, 881, 1996, 2366
Bst6I CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
BstACI GRCGYC 1 cut(s) 75
BstBI TTCGAA 1 cut(s) 1059
BstC8I GCNNGC 7 cut(s) 1002, 1239, 1521, 1591, 1595, 1934, 2569
BstDSI CCRYGG 2 cut(s) 764, 1138
BstENI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
BstF5I GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BstFNI CGCG 2 cut(s) 766, 2336
BstHHI GCGC 2 cut(s) 1736, 2338
BstMAI GTCTC 2 cut(s) 82, 1318
BstMWI GCNNNNNNNGC 5 cut(s) 599, 970, 1143, 1152, 2504
BstNI CCWGG 5 cut(s) 471, 1272, 1320, 1954, 2453
BstNSI RCATGY 7 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359
BstSCI CCNGG 7 cut(s) 402, 469, 1270, 1318, 1908, 1952, 2451
BstSFI CTRYAG 3 cut(s) 595, 705, 910
BstSLI GKGCMC 1 cut(s) 683
BstUI CGCG 2 cut(s) 766, 2336
BstV1I GCAGC 4 cut(s) 252, 1609, 2180, 2551
BstV2I GAAGAC 3 cut(s) 450, 650, 1088
BstX2I RGATCY 2 cut(s) 1675, 1687
BstXI CCANNNNNNTGG 1 cut(s) 346
BstYI RGATCY 2 cut(s) 1675, 1687
BsuRI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BtgI CCRYGG 2 cut(s) 764, 1138
BtsCI GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BtsI GCAGTG 4 cut(s) 481, 1126, 1162, 1729
BtsIMutI CAGTG 6 cut(s) 481, 1041, 1126, 1162, 1449, 1729
Cac8I GCNNGC 7 cut(s) 1002, 1239, 1521, 1591, 1595, 1934, 2569
CaiI CAGNNNCTG 2 cut(s) 1078, 1959
CciI TCATGA 1 cut(s) 489
CfoI GCGC 2 cut(s) 1736, 2338
Cfr10I RCCGGY 1 cut(s) 2302
Cfr13I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
Cfr42I CCGCGG 1 cut(s) 767
CpoI CGGWCCG 1 cut(s) 48
CseI GACGC 1 cut(s) 516
Csp6I GTAC 4 cut(s) 1917, 2194, 2392, 2400
CspI CGGWCCG 1 cut(s) 48
CviQI GTAC 4 cut(s) 1917, 2194, 2392, 2400
DraI TTTAAA 1 cut(s) 1210
DrdI GACNNNNNNGTC 2 cut(s) 331, 2242
DriI GACNNNNNGTC 1 cut(s) 2158
DseDI GACNNNNNNGTC 2 cut(s) 331, 2242
Eam1104I CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
Eam1105I GACNNNNNGTC 1 cut(s) 2158
EarI CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
EciI GGCGGA 1 cut(s) 2216
Ecl136II GAGCTC 1 cut(s) 2224
Eco130I CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
Eco147I AGGCCT 1 cut(s) 1181
Eco24I GRGCYC 1 cut(s) 2226
Eco32I GATATC 1 cut(s) 1099
Eco47I GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
Eco53kI GAGCTC 1 cut(s) 2224
Eco57I CTGAAG 8 cut(s) 141, 842, 1055, 1100, 1458, 1541, 2184, 2208
EcoICRI GAGCTC 1 cut(s) 2224
EcoNI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
EcoO109I RGGNCCY 4 cut(s) 817, 932, 1024, 2068
EcoRII CCWGG 5 cut(s) 469, 1270, 1318, 1952, 2451
EcoRV GATATC 1 cut(s) 1099
EcoT14I CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 2226
ErhI CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
Esp3I CGTCTC 1 cut(s) 82
FalI AAGNNNNNCTT 2 cut(s) 2157, 2189
FauI CCCGC 2 cut(s) 771, 1364
FauNDI CATATG 2 cut(s) 418, 1722
FbaI TGATCA 2 cut(s) 1393, 2424
FblI GTMKAC 3 cut(s) 335, 1701, 2202
Fnu4HI GCNGC 4 cut(s) 266, 1598, 2169, 2565
FokI GGATG 9 cut(s) 453, 701, 821, 1232, 1374, 1478, 1765, 1925, 2252
FriOI GRGCYC 1 cut(s) 2226
Fsp4HI GCNGC 4 cut(s) 266, 1598, 2169, 2565
FspBI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
GlaI GCGC 2 cut(s) 1735, 2337
GluI GCNGC 4 cut(s) 266, 1598, 2169, 2565
GsuI CTGGAG 2 cut(s) 1056, 1473
HaeIII GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
HapII CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
HgaI GACGC 1 cut(s) 516
HhaI GCGC 2 cut(s) 1736, 2338
Hin1I GRCGYC 1 cut(s) 75
Hin6I GCGC 2 cut(s) 1734, 2336
HinP1I GCGC 2 cut(s) 1734, 2336
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 2 cut(s) 89, 1898
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
HphI GGTGA 7 cut(s) 150, 1118, 1202, 1308, 1366, 1667, 2463
Hpy166II GTNNAC 6 cut(s) 336, 664, 681, 1702, 1906, 2203
Hpy8I GTNNAC 6 cut(s) 336, 664, 681, 1702, 1906, 2203
Hpy99I CGWCG 1 cut(s) 532
HpyAV CCTTC 8 cut(s) 743, 817, 954, 1003, 1037, 1103, 1171, 1192
HpyCH4III ACNGT 6 cut(s) 449, 649, 706, 881, 1996, 2366
HpyCH4IV ACGT 2 cut(s) 75, 2196
HpyF10VI GCNNNNNNNGC 5 cut(s) 599, 970, 1143, 1152, 2504
HpySE526I ACGT 2 cut(s) 75, 2196
Hsp92I GRCGYC 1 cut(s) 75
HspAI GCGC 2 cut(s) 1734, 2336
KflI GGGWCCC 1 cut(s) 2068
Ksp22I TGATCA 2 cut(s) 1393, 2424
KspAI GTTAAC 1 cut(s) 664
KspI CCGCGG 1 cut(s) 767
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 5 cut(s) 198, 946, 1516, 2039, 2229
Lsp1109I GCAGC 4 cut(s) 252, 1609, 2180, 2551
LweI GCATC 4 cut(s) 679, 807, 928, 2239
MaeI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
MaeII ACGT 2 cut(s) 75, 2196
MaeIII GTNAC 9 cut(s) 136, 146, 290, 1039, 1190, 1655, 1707, 1859, 2469
MfeI CAATTG 3 cut(s) 987, 2047, 2340
MflI RGATCY 2 cut(s) 1675, 1687
MhlI GDGCHC 3 cut(s) 393, 683, 2226
MmeI TCCRAC 2 cut(s) 220, 2367
Mph1103I ATGCAT 1 cut(s) 694
MroXI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
MseI TTAA 8 cut(s) 663, 701, 1209, 1893, 2016, 2256, 2328, 2433
MslI CAYNNNNRTG 6 cut(s) 222, 344, 803, 1751, 1803, 2136
MspA1I CMGCKG 1 cut(s) 766
MspI CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
MspR9I CCNGG 7 cut(s) 404, 471, 1272, 1320, 1910, 1954, 2453
MunI CAATTG 3 cut(s) 987, 2047, 2340
Mva1269I GAATGC 2 cut(s) 692, 1139
MvaI CCWGG 5 cut(s) 471, 1272, 1320, 1954, 2453
MvnI CGCG 2 cut(s) 766, 2336
MwoI GCNNNNNNNGC 5 cut(s) 599, 970, 1143, 1152, 2504
NciI CCSGG 2 cut(s) 404, 1910
NdeI CATATG 2 cut(s) 418, 1722
NheI GCTAGC 1 cut(s) 1589
NlaIV GGNNCC 9 cut(s) 6, 252, 651, 933, 1677, 1845, 2069, 2070, 2153
NmeAIII GCCGAG 1 cut(s) 526
NmuCI GTSAC 6 cut(s) 146, 1039, 1190, 1655, 1859, 2469
NsiI ATGCAT 1 cut(s) 694
NspI RCATGY 7 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359
NspV TTCGAA 1 cut(s) 1059
OliI CACNNNNGTG 1 cut(s) 222
PaeI GCATGC 3 cut(s) 1004, 1241, 1597
PagI TCATGA 1 cut(s) 489
PceI AGGCCT 1 cut(s) 1181
PciI ACATGT 3 cut(s) 1782, 2131, 2355
PciSI GCTCTTC 1 cut(s) 468
PctI GAATGC 2 cut(s) 692, 1139
PdmI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
PfeI GAWTC 2 cut(s) 106, 237
PflFI GACNNNGTC 1 cut(s) 485
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 4 cut(s) 267, 1599, 2170, 2566
PpuMI RGGWCCY 3 cut(s) 817, 932, 2068
PscI ACATGT 3 cut(s) 1782, 2131, 2355
PsiI TTATAA 1 cut(s) 425
Psp124BI GAGCTC 1 cut(s) 2226
Psp5II RGGWCCY 3 cut(s) 817, 932, 2068
Psp6I CCWGG 5 cut(s) 469, 1270, 1318, 1952, 2451
PspGI CCWGG 5 cut(s) 469, 1270, 1318, 1952, 2451
PspN4I GGNNCC 9 cut(s) 6, 252, 651, 933, 1677, 1845, 2069, 2070, 2153
PspPI GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
PspPPI RGGWCCY 3 cut(s) 817, 932, 2068
PstNI CAGNNNCTG 2 cut(s) 1078, 1959
PsuI RGATCY 2 cut(s) 1675, 1687
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 4 cut(s) 1918, 2195, 2393, 2401
RsaNI GTAC 4 cut(s) 1917, 2194, 2392, 2400
RseI CAYNNNNRTG 6 cut(s) 222, 344, 803, 1751, 1803, 2136
Rsr2I CGGWCCG 1 cut(s) 48
RsrII CGGWCCG 1 cut(s) 48
SacI GAGCTC 1 cut(s) 2226
SacII CCGCGG 1 cut(s) 767
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 8 cut(s) 663, 701, 1209, 1893, 2016, 2256, 2328, 2433
SatI GCNGC 4 cut(s) 266, 1598, 2169, 2565
Sau96I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
ScrFI CCNGG 7 cut(s) 404, 471, 1272, 1320, 1910, 1954, 2453
SduI GDGCHC 3 cut(s) 393, 683, 2226
SfaNI GCATC 4 cut(s) 679, 807, 928, 2239
SfcI CTRYAG 3 cut(s) 595, 705, 910
Sfr303I CCGCGG 1 cut(s) 767
SfuI TTCGAA 1 cut(s) 1059
SgrBI CCGCGG 1 cut(s) 767
SinI GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
SmiMI CAYNNNNRTG 6 cut(s) 222, 344, 803, 1751, 1803, 2136
SmlI CTYRAG 3 cut(s) 1195, 2461, 2539
SmoI CTYRAG 3 cut(s) 1195, 2461, 2539
SphI GCATGC 3 cut(s) 1004, 1241, 1597
SseBI AGGCCT 1 cut(s) 1181
SsiI CCGC 7 cut(s) 753, 764, 766, 1371, 2028, 2227, 2403
SspMI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
SstI GAGCTC 1 cut(s) 2226
StuI AGGCCT 1 cut(s) 1181
StyD4I CCNGG 7 cut(s) 402, 469, 1270, 1318, 1908, 1952, 2451
StyI CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
TaaI ACNGT 6 cut(s) 449, 649, 706, 881, 1996, 2366
TaiI ACGT 2 cut(s) 78, 2199
TaqI TCGA 4 cut(s) 530, 1059, 1884, 2409
TatI WGTACW 1 cut(s) 2391
TfiI GAWTC 2 cut(s) 106, 237
Tru1I TTAA 8 cut(s) 663, 701, 1209, 1893, 2016, 2256, 2328, 2433
Tru9I TTAA 8 cut(s) 663, 701, 1209, 1893, 2016, 2256, 2328, 2433
TscAI CASTG 6 cut(s) 481, 1048, 1126, 1162, 1456, 1736
TseFI GTSAC 6 cut(s) 146, 1039, 1190, 1655, 1859, 2469
TseI GCWGC 4 cut(s) 265, 1597, 2168, 2564
Tsp45I GTSAC 6 cut(s) 146, 1039, 1190, 1655, 1859, 2469
TspGWI ACGGA 1 cut(s) 1518
TspRI CASTG 6 cut(s) 481, 1048, 1126, 1162, 1456, 1736
Tth111I GACNNNGTC 1 cut(s) 485
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
XagI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
XapI RAATTY 5 cut(s) 532, 668, 740, 1811, 2004
XceI RCATGY 7 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359
XmiI GTMKAC 3 cut(s) 335, 1701, 2202
XmnI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
XspI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
ZraI GACGTC 1 cut(s) 76
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.