MD09G1285100.v1.1

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002202
Physical Location & Seq
Forward (+)
36308267 .. 36311531
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1285100.v1.1.491

Sequence Viewer

Length: 2604 bp
ATGGAACCTCCTGATGATCCTGTTAATCCGATCCGATTCGCGTGTCGGTCTGACAGTTTAGAACAGTACTTTAATTTGTCTAATGGAAAGTTTAGTGTCAACGGTGTTCCGTTGCTCACTGAAGTTCCAAGCAATGTCACTTTTAATCACTTCCACTCAATCTTCCAGTCTTCTGATGCTCCACTTCCTGTGCTCCAACGCGTTCGCGCCTTGTCCCACAAGGGCGGGTTCCTTGGATTCAGGAAGGAGGAGCCTTCGGATAGGTTGATGAACTCCTTAGGTAAATTTAGCAACAGGGATTTCCTTAGCATCTTTAGGTTCAAGACATGGTGGTCCACCATGTTTGTGGGAAGTTCAGGGTCAAATCTGCAAATGGAAACCCAATGGGTGCTCTTTGATGTCCCGGAAATTAAGTCCTATGTTATAATCTTGCCTGTTATCGAAGGCAACTTTAGGTCTGCTCTTCATCCTGGCACTGATGATCATGTCATGATCTGTGCTGAGAGTGGTTCTACCCAAGTGAAAGCATCGAATTTCGATGCCATCGCTTATGTCCATGTTTCGGACAATCCCTACAACTTGATGAAAGAAGCATATAGTGCTCTTAGGGTCCATCTTAATACCTTTCGGCTGTTGGAAGAGAAGACGGTCCCCAATCTGGTTGACAAGTTTGGTTGGTGCACTTGGGATGCCTTCTACTTAACTGTAGAGCCTGTTGGTGTCTGGCATGGTGTCAACGAATTTGTCGAAGGTGGTGTATCCCCTAGGTTTCTCATCATTGATGATGGCTGGCAAAGCATCAATTTGGATGATGAGGATGTGAATGAGGATGCGAAAAATCTAGTACTTGGCGGGACTCAAATGACTGCCAGGCTTTACAGGTTTGACGAGTGCAAGAAGTTCAGGGAGTACAGGGGCGGTTCGATGTTGGCGCCGGATGCTCCTTCGTTTGATCCCAAAAAGCCAAAGTTGTTGATAGCCAAGGCAATTGAAATAGAACACGCTGAGAAGAATCGTGACATGGCCATTCGATCTGGAGCCACTGACTTGTCCGATTTTGAAACAAAAATTCAGAAGCTGAAGCAAGAGTTGAATGAGATCCTTGGCAAGGAAGAAAGCACTGCTTCTAATGAAGGCTGCGGAAGCTGTTCTTGCAGCGCCAATAACTACGGAATGAAGGCTTTCACGAAGGATTTGAGAACCAAATTTAAAGGCTTGGACGATATATACGTATGGCATGCTCTCTGTGGTGCCTGGGGTGGTGTTAGGCCAGGTTCAACACATCTTAAATCAAAGATCACTCCATGTAAAGTCTCTCCGGGACTTGATGGTACCATGACTGACCTTGCTGTGGTGAAAATCGTTGAGGGCGGAATTGGACTTGTTCATCCTGATCAAGCTGATAATTTGTATGACGCGATGCACTCCTACCTTTCCAAAGTTGGCATCACAGGAGTTAAAGTGGATGTTATTCAAACTCTCGAGTATGTATCTGAGGAGTATGGAGGCCGCGTGGAGCTTGCGAAGGCGTATTACAAGGGGTTGTCTCGCTCTCTTCAGAACAACTTCAAAGGGAATGGACTAATTGCTAGCATGCAGCAGTGCAACGATTTCTTCTTCCTAGGGACAAAGCAAATCTCTTTTGGAAGAGTTGGTGATGATTTTTGGTTCCAAGATCCGAATGGAGATCCGATGGGAGTTTATTGGTTACAAGGTGTTCATATGATCCATTGTGCCTACAACAGCATGTGGATGGGTCAGATCATACATCCCGATTGGGATATGTTCCAATCAGACCATCTGTGTGCCAAATTTCATGCAGGATCTAGAGCTATCTGTGGAGGTCCGGTGTATGTAAGTGACTCAGTTTCCGGCCATGATTTTGATCTCATTAAGAAGCTTGTTTACCCGGATGGTACCATTCCCAAGTGCCAGCATTTTGCCCTCCCCACTAGGGACTGCCTCTTCAAAAACCCTTTATTTGACAACAAAACTGTTCTTAAAATTTGGAACTTCAACAAGCATGGTGGTGTGATTGGAGCTTTCAACTGCCAAGGGGCTGGTTGGGACCCCAAAGAGCAAAGGATCCGAGGATACTCAGAGTGCTATAACCTGAAATCTTGTTCACTGCATGTTTCCGAAATTGAATGGGACCAAAAGATAGAAGCAGCACATATGGGCAAGGCTGAAGGGTACGTAGTCTATCTCAACCAGGCTGATGAACTCCGCCTAATGACCCCAAAATCTGATGCAATTCAAGTTATCATCCAGCCATCTTCGTTCGAGCTTTTCAGCTTCGTGCCAATTAAAAAGCTCGGACGCAACATCAAATTCGCACCAATTGGACTTACAAACATGTTCAACAGTGGAGGTGCCCTCCAAGAGTTGGAGTACAAGACTAGTGCAGCTGAGGTTAGTGCCATGGTGAAGGTGAAGGTCAAAGGTGGAGGCAATTTCTTGGCCTACTCAAGTGAGTCTCCAAAGAAATGTTATTTGAATGGTGCTGAAGTGGCTTTCGAGTGGTCGGCCGAGGGTAAACTGGCTCTGAACCTTGCTTGGGTTGAAGAAGCTGCTGGCATTTCTGAAGTGGTTTTTGCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

868

Amino Acids

96.7

Weight (kDa)

5.9

Isoelectric Point (pI)

31.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 850 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AasI GACNNNNNNGTC 1 cut(s) 331
Acc65I GGTACC 2 cut(s) 1331, 1916
AccB1I GGYRCC 5 cut(s) 931, 1250, 1331, 1916, 2372
AccB7I CCANNNNNTGG 1 cut(s) 2386
AccII CGCG 5 cut(s) 41, 201, 207, 1418, 1512
AciI CCGC 7 cut(s) 225, 852, 918, 1140, 1371, 1510, 2227
AcoI YGGCCR 3 cut(s) 1023, 1873, 2526
AcsI RAATTY 8 cut(s) 284, 532, 740, 1068, 1205, 1811, 2004, 2330
AcuI CTGAAG 6 cut(s) 141, 1100, 1541, 2208, 2526, 2604
AcyI GRCGYC 1 cut(s) 932
AfaI GTAC 7 cut(s) 68, 846, 911, 1333, 1918, 2195, 2393
AfiI CCNNNNNNNGG 7 cut(s) 562, 658, 1108, 1351, 1954, 2386, 2557
AflIII ACRYGT 2 cut(s) 199, 2355
AhlI ACTAGT 1 cut(s) 2399
AjnI CCWGG 5 cut(s) 469, 869, 1253, 1270, 2211
Alw21I GWGCWC 4 cut(s) 195, 393, 604, 683
Alw26I GTCTC 3 cut(s) 1318, 1551, 2481
Alw44I GTGCAC 1 cut(s) 679
AlwNI CAGNNNCTG 1 cut(s) 1078
Ama87I CYCGRG 1 cut(s) 1481
AoxI GGCC 6 cut(s) 1023, 1268, 1507, 1873, 2460, 2526
ApaLI GTGCAC 1 cut(s) 679
ApeKI GCWGC 6 cut(s) 1137, 1155, 1597, 2168, 2405, 2570
ApoI RAATTY 8 cut(s) 284, 532, 740, 1068, 1205, 1811, 2004, 2330
Asp700I GAANNNNTTC 3 cut(s) 1147, 1181, 1565
Asp718I GGTACC 2 cut(s) 1331, 1916
AspA2I CCTAGG 2 cut(s) 764, 1621
AspLEI GCGC 3 cut(s) 209, 934, 1160
AspS9I GGNCC 6 cut(s) 333, 610, 649, 1844, 2068, 2152
AsuC2I CCSGG 3 cut(s) 404, 1320, 1910
AsuHPI GGTGA 4 cut(s) 1366, 1667, 2437, 2443
AsuNHI GCTAGC 1 cut(s) 1589
AvaI CYCGRG 1 cut(s) 1481
AvaII GGWCC 6 cut(s) 333, 610, 649, 1844, 2068, 2152
AvrII CCTAGG 2 cut(s) 764, 1621
AxyI CCTNAGG 1 cut(s) 277
BaeGI GKGCMC 2 cut(s) 683, 2377
BalI TGGCCA 1 cut(s) 1025
BamHI GGATCC 1 cut(s) 2085
BanI GGYRCC 5 cut(s) 931, 1250, 1331, 1916, 2372
BarI GAAGNNNNNNTAC 4 cut(s) 1517, 1549, 1889, 1921
BbsI GAAGAC 2 cut(s) 162, 650
Bbv12I GWGCWC 4 cut(s) 195, 393, 604, 683
BbvCI CCTCAGC 1 cut(s) 2409
BbvI GCAGC 6 cut(s) 1124, 1167, 1609, 2180, 2417, 2557
BccI CCATC 9 cut(s) 551, 621, 779, 1322, 1687, 1747, 1806, 1907, 2281
BciT130I CCWGG 5 cut(s) 471, 871, 1255, 1272, 2213
BciVI GTATCC 2 cut(s) 769, 2087
BclI TGATCA 2 cut(s) 481, 1393
BcnI CCSGG 3 cut(s) 404, 1320, 1910
BcoDI GTCTC 3 cut(s) 1318, 1551, 2481
BcuI ACTAGT 1 cut(s) 2399
BfaI CTAG 7 cut(s) 765, 842, 1590, 1622, 1827, 1953, 2400
BfmI CTRYAG 1 cut(s) 705
BfoI RGCGCY 2 cut(s) 935, 1161
BfuI GTATCC 2 cut(s) 769, 2087
BisI GCNGC 7 cut(s) 1138, 1156, 1510, 1598, 2169, 2406, 2571
BlnI CCTAGG 2 cut(s) 764, 1621
BlsI GCNGC 7 cut(s) 1139, 1157, 1511, 1599, 2170, 2407, 2572
BmcAI AGTACT 2 cut(s) 68, 846
Bme1390I CCNGG 8 cut(s) 404, 471, 871, 1255, 1272, 1320, 1910, 2213
Bme18I GGWCC 6 cut(s) 333, 610, 649, 1844, 2068, 2152
BmeT110I CYCGRG 1 cut(s) 1481
BmgT120I GGNCC 6 cut(s) 333, 610, 649, 1844, 2068, 2152
BmrFI CCNGG 8 cut(s) 404, 471, 871, 1255, 1272, 1320, 1910, 2213
BmtI GCTAGC 1 cut(s) 1593
BpiI GAAGAC 2 cut(s) 162, 650
BplI GAGNNNNNCTC 2 cut(s) 2361, 2393
BpmI CTGGAG 1 cut(s) 1056
Bpu10I CCTNAGC 2 cut(s) 305, 2409
BpuEI CTTGAG 1 cut(s) 2452
BpuMI CCSGG 3 cut(s) 404, 1320, 1910
BsaAI YACGTR 2 cut(s) 1231, 2197
BsaBI GATNNNNATC 1 cut(s) 1884
BsaHI GRCGYC 1 cut(s) 932
BsaWI WCCGGW 1 cut(s) 1846
BsaXI ACNNNNNCTCC 6 cut(s) 1446, 1476, 1497, 1527, 1833, 1863
Bsc4I CCNNNNNNNGG 7 cut(s) 562, 658, 1108, 1351, 1954, 2386, 2557
Bse1I ACTGG 2 cut(s) 166, 2544
Bse21I CCTNAGG 1 cut(s) 277
Bse3DI GCAATG 1 cut(s) 139
Bse8I GATNNNNATC 1 cut(s) 1884
BseBI CCWGG 5 cut(s) 471, 871, 1255, 1272, 2213
BseJI GATNNNNATC 1 cut(s) 1884
BseLI CCNNNNNNNGG 7 cut(s) 562, 658, 1108, 1351, 1954, 2386, 2557
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 6 cut(s) 492, 996, 1485, 1878, 2112, 2400
BseNI ACTGG 2 cut(s) 166, 2544
BseRI GAGGAG 2 cut(s) 263, 1511
BseSI GKGCMC 2 cut(s) 683, 2377
BseX3I CGGCCG 1 cut(s) 2526
BseXI GCAGC 6 cut(s) 1124, 1167, 1609, 2180, 2417, 2557
BsgI GTGCAG 1 cut(s) 2424
Bsh1236I CGCG 5 cut(s) 41, 201, 207, 1418, 1512
Bsh1285I CGRYCG 1 cut(s) 2529
BshFI GGCC 6 cut(s) 1025, 1270, 1509, 1875, 2462, 2528
BshNI GGYRCC 5 cut(s) 931, 1250, 1331, 1916, 2372
BsiEI CGRYCG 1 cut(s) 2529
BsiHKAI GWGCWC 4 cut(s) 195, 393, 604, 683
BsiHKCI CYCGRG 1 cut(s) 1481
BsiSI CCGG 6 cut(s) 404, 935, 1319, 1847, 1872, 1910
BslFI GGGAC 9 cut(s) 199, 386, 635, 868, 1335, 1639, 1970, 2081, 2165
BslI CCNNNNNNNGG 7 cut(s) 562, 658, 1108, 1351, 1954, 2386, 2557
BsmAI GTCTC 3 cut(s) 1318, 1551, 2481
BsmFI GGGAC 9 cut(s) 199, 386, 635, 868, 1335, 1639, 1970, 2081, 2165
BsnI GGCC 6 cut(s) 1025, 1270, 1509, 1875, 2462, 2528
BsoBI CYCGRG 1 cut(s) 1481
Bsp1286I GDGCHC 5 cut(s) 195, 393, 604, 683, 2377
Bsp19I CCATGG 1 cut(s) 2421
BspACI CCGC 7 cut(s) 225, 852, 918, 1140, 1371, 1510, 2227
BspANI GGCC 6 cut(s) 1025, 1270, 1509, 1875, 2462, 2528
BspCNI CTCAG 6 cut(s) 493, 997, 1486, 1877, 2111, 2401
BspFNI CGCG 5 cut(s) 41, 201, 207, 1418, 1512
BspHI TCATGA 1 cut(s) 489
BspOI GCTAGC 1 cut(s) 1593
BspQI GCTCTTC 1 cut(s) 468
BspT107I GGYRCC 5 cut(s) 931, 1250, 1331, 1916, 2372
BsrDI GCAATG 1 cut(s) 139
BsrI ACTGG 2 cut(s) 166, 2544
BssNI GRCGYC 1 cut(s) 932
BssT1I CCWWGG 7 cut(s) 232, 764, 981, 1102, 1621, 2053, 2421
Bst2UI CCWGG 5 cut(s) 471, 871, 1255, 1272, 2213
Bst4CI ACNGT 7 cut(s) 56, 66, 104, 649, 706, 1996, 2366
Bst6I CTCTTC 5 cut(s) 468, 633, 1560, 1642, 1970
BstACI GRCGYC 1 cut(s) 932
BstAPI GCANNNNNTGC 1 cut(s) 599
BstBAI YACGTR 2 cut(s) 1231, 2197
BstC8I GCNNGC 7 cut(s) 791, 1239, 1521, 1591, 1595, 1934, 2575
BstDEI CTNAG 9 cut(s) 277, 305, 501, 605, 1005, 1494, 1864, 2098, 2409
BstDSI CCRYGG 1 cut(s) 2421
BstENI CCTNNNNNAGG 1 cut(s) 1106
BstFNI CGCG 5 cut(s) 41, 201, 207, 1418, 1512
BstH2I RGCGCY 2 cut(s) 935, 1161
BstHHI GCGC 3 cut(s) 209, 934, 1160
BstMAI GTCTC 3 cut(s) 1318, 1551, 2481
BstMCI CGRYCG 1 cut(s) 2529
BstMWI GCNNNNNNNGC 6 cut(s) 599, 795, 938, 1143, 1152, 2510
BstNI CCWGG 5 cut(s) 471, 871, 1255, 1272, 2213
BstNSI RCATGY 5 cut(s) 1241, 1597, 1750, 2135, 2359
BstSCI CCNGG 8 cut(s) 402, 469, 869, 1253, 1270, 1318, 1908, 2211
BstSFI CTRYAG 1 cut(s) 705
BstSLI GKGCMC 2 cut(s) 683, 2377
BstSNI TACGTA 2 cut(s) 1231, 2197
BstUI CGCG 5 cut(s) 41, 201, 207, 1418, 1512
BstV1I GCAGC 6 cut(s) 1124, 1167, 1609, 2180, 2417, 2557
BstV2I GAAGAC 2 cut(s) 162, 650
BstX2I RGATCY 5 cut(s) 1098, 1675, 1687, 1823, 2085
BstXI CCANNNNNNTGG 2 cut(s) 346, 2060
BstYI RGATCY 5 cut(s) 1098, 1675, 1687, 1823, 2085
BstZI CGGCCG 1 cut(s) 2526
Bsu36I CCTNAGG 1 cut(s) 277
BsuI GTATCC 2 cut(s) 769, 2087
BsuRI GGCC 6 cut(s) 1025, 1270, 1509, 1875, 2462, 2528
BtgI CCRYGG 1 cut(s) 2421
BtgZI GCGATG 2 cut(s) 529, 1433
BtsI GCAGTG 3 cut(s) 1119, 1607, 2126
BtsIMutI CAGTG 7 cut(s) 117, 474, 1041, 1119, 1607, 2126, 2371
Cac8I GCNNGC 7 cut(s) 791, 1239, 1521, 1591, 1595, 1934, 2575
CaiI CAGNNNCTG 1 cut(s) 1078
CciI TCATGA 1 cut(s) 489
CfoI GCGC 3 cut(s) 209, 934, 1160
Cfr13I GGNCC 6 cut(s) 333, 610, 649, 1844, 2068, 2152
CseI GACGC 2 cut(s) 1424, 2328
Csp6I GTAC 7 cut(s) 67, 845, 910, 1332, 1917, 2194, 2392
CspCI CAANNNNNGTGG 4 cut(s) 325, 360, 2008, 2043
CviQI GTAC 7 cut(s) 67, 845, 910, 1332, 1917, 2194, 2392
DdeI CTNAG 9 cut(s) 277, 305, 501, 605, 1005, 1494, 1864, 2098, 2409
DinI GGCGCC 1 cut(s) 933
DraI TTTAAA 1 cut(s) 1210
DrdI GACNNNNNNGTC 1 cut(s) 331
DseDI GACNNNNNNGTC 1 cut(s) 331
EaeI YGGCCR 3 cut(s) 1023, 1873, 2526
EagI CGGCCG 1 cut(s) 2526
Eam1104I CTCTTC 5 cut(s) 468, 633, 1560, 1642, 1970
EarI CTCTTC 5 cut(s) 468, 633, 1560, 1642, 1970
EciI GGCGGA 2 cut(s) 1386, 2216
EclXI CGGCCG 1 cut(s) 2526
Eco105I TACGTA 2 cut(s) 1231, 2197
Eco130I CCWWGG 7 cut(s) 232, 764, 981, 1102, 1621, 2053, 2421
Eco47I GGWCC 6 cut(s) 333, 610, 649, 1844, 2068, 2152
Eco52I CGGCCG 1 cut(s) 2526
Eco57I CTGAAG 6 cut(s) 141, 1100, 1541, 2208, 2526, 2604
Eco81I CCTNAGG 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 1481
EcoNI CCTNNNNNAGG 1 cut(s) 1106
EcoO109I RGGNCCY 1 cut(s) 2068
EcoRII CCWGG 5 cut(s) 469, 869, 1253, 1270, 2211
EcoT14I CCWWGG 7 cut(s) 232, 764, 981, 1102, 1621, 2053, 2421
EgeI GGCGCC 1 cut(s) 933
EheI GGCGCC 1 cut(s) 933
ErhI CCWWGG 7 cut(s) 232, 764, 981, 1102, 1621, 2053, 2421
FalI AAGNNNNNCTT 4 cut(s) 1108, 1140, 1135, 1167
FaqI GGGAC 9 cut(s) 199, 386, 635, 868, 1335, 1639, 1970, 2081, 2165
FauI CCCGC 2 cut(s) 218, 845
FauNDI CATATG 2 cut(s) 1722, 2175
FbaI TGATCA 2 cut(s) 481, 1393
Fnu4HI GCNGC 7 cut(s) 1138, 1156, 1510, 1598, 2169, 2406, 2571
Fsp4HI GCNGC 7 cut(s) 1138, 1156, 1510, 1598, 2169, 2406, 2571
FspBI CTAG 7 cut(s) 765, 842, 1590, 1622, 1827, 1953, 2400
GlaI GCGC 3 cut(s) 208, 933, 1159
GluI GCNGC 7 cut(s) 1138, 1156, 1510, 1598, 2169, 2406, 2571
GsuI CTGGAG 1 cut(s) 1056
HaeII RGCGCY 2 cut(s) 935, 1161
HaeIII GGCC 6 cut(s) 1025, 1270, 1509, 1875, 2462, 2528
HapII CCGG 6 cut(s) 404, 935, 1319, 1847, 1872, 1910
HgaI GACGC 2 cut(s) 1424, 2328
HhaI GCGC 3 cut(s) 209, 934, 1160
Hin1I GRCGYC 1 cut(s) 932
Hin6I GCGC 3 cut(s) 207, 932, 1158
HinP1I GCGC 3 cut(s) 207, 932, 1158
HincII GTYRAC 3 cut(s) 100, 664, 736
HindII GTYRAC 3 cut(s) 100, 664, 736
HindIII AAGCTT 1 cut(s) 1898
HinfI GANTC 6 cut(s) 36, 237, 856, 1012, 1862, 2474
HpaII CCGG 6 cut(s) 404, 935, 1319, 1847, 1872, 1910
HphI GGTGA 4 cut(s) 1366, 1667, 2437, 2443
Hpy166II GTNNAC 8 cut(s) 100, 336, 664, 681, 736, 1906, 2126, 2537
Hpy8I GTNNAC 8 cut(s) 100, 336, 664, 681, 736, 1906, 2126, 2537
HpyCH4III ACNGT 7 cut(s) 56, 66, 104, 649, 706, 1996, 2366
HpyCH4IV ACGT 2 cut(s) 1230, 2196
HpyF10VI GCNNNNNNNGC 6 cut(s) 599, 795, 938, 1143, 1152, 2510
HpyF3I CTNAG 9 cut(s) 277, 305, 501, 605, 1005, 1494, 1864, 2098, 2409
HpySE526I ACGT 2 cut(s) 1230, 2196
Hsp92I GRCGYC 1 cut(s) 932
HspAI GCGC 3 cut(s) 207, 932, 1158
KasI GGCGCC 1 cut(s) 931
KflI GGGWCCC 1 cut(s) 2068
KpnI GGTACC 2 cut(s) 1335, 1920
Ksp22I TGATCA 2 cut(s) 481, 1393
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 7 cut(s) 184, 198, 250, 946, 1037, 1516, 2039
Lsp1109I GCAGC 6 cut(s) 1124, 1167, 1609, 2180, 2417, 2557
MaeI CTAG 7 cut(s) 765, 842, 1590, 1622, 1827, 1953, 2400
MaeII ACGT 2 cut(s) 1230, 2196
MaeIII GTNAC 4 cut(s) 136, 1016, 1707, 1859
MfeI CAATTG 2 cut(s) 987, 2340
MflI RGATCY 5 cut(s) 1098, 1675, 1687, 1823, 2085
MhlI GDGCHC 5 cut(s) 195, 393, 604, 683, 2377
MlsI TGGCCA 1 cut(s) 1025
MluI ACGCGT 1 cut(s) 199
MluNI TGGCCA 1 cut(s) 1025
Mly113I GGCGCC 1 cut(s) 932
MlyI GAGTC 3 cut(s) 850, 1856, 2483
MmeI TCCRAC 3 cut(s) 220, 615, 2367
Mox20I TGGCCA 1 cut(s) 1025
MroXI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
MscI TGGCCA 1 cut(s) 1025
MslI CAYNNNNRTG 4 cut(s) 344, 1751, 1803, 2028
Msp20I TGGCCA 1 cut(s) 1025
MspA1I CMGCKG 1 cut(s) 2408
MspI CCGG 6 cut(s) 404, 935, 1319, 1847, 1872, 1910
MspR9I CCNGG 8 cut(s) 404, 471, 871, 1255, 1272, 1320, 1910, 2213
MunI CAATTG 2 cut(s) 987, 2340
MvaI CCWGG 5 cut(s) 471, 871, 1255, 1272, 2213
MvnI CGCG 5 cut(s) 41, 201, 207, 1418, 1512
MwoI GCNNNNNNNGC 6 cut(s) 599, 795, 938, 1143, 1152, 2510
NarI GGCGCC 1 cut(s) 932
NciI CCSGG 3 cut(s) 404, 1320, 1910
NcoI CCATGG 1 cut(s) 2421
NdeI CATATG 2 cut(s) 1722, 2175
NheI GCTAGC 1 cut(s) 1589
NmeAIII GCCGAG 1 cut(s) 2554
NmuCI GTSAC 3 cut(s) 136, 1016, 1859
NspI RCATGY 5 cut(s) 1241, 1597, 1750, 2135, 2359
PaeI GCATGC 2 cut(s) 1241, 1597
PaeR7I CTCGAG 1 cut(s) 1481
PagI TCATGA 1 cut(s) 489
PciI ACATGT 1 cut(s) 2355
PciSI GCTCTTC 1 cut(s) 468
PcsI WCGNNNNNNNCGW 2 cut(s) 744, 1227
PdmI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
PfeI GAWTC 3 cut(s) 36, 237, 1012
PflMI CCANNNNNTGG 1 cut(s) 2386
PfoI TCCNGGA 2 cut(s) 402, 1318
PkrI GCNGC 7 cut(s) 1139, 1157, 1511, 1599, 2170, 2407, 2572
PleI GAGTC 3 cut(s) 850, 1856, 2482
PluTI GGCGCC 1 cut(s) 935
PpsI GAGTC 3 cut(s) 850, 1856, 2482
Ppu21I YACGTR 2 cut(s) 1231, 2197
PpuMI RGGWCCY 1 cut(s) 2068
PscI ACATGT 1 cut(s) 2355
PsiI TTATAA 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 2068
Psp6I CCWGG 5 cut(s) 469, 869, 1253, 1270, 2211
PspGI CCWGG 5 cut(s) 469, 869, 1253, 1270, 2211
PspPI GGNCC 6 cut(s) 333, 610, 649, 1844, 2068, 2152
PspPPI RGGWCCY 1 cut(s) 2068
PstNI CAGNNNCTG 1 cut(s) 1078
PsuI RGATCY 5 cut(s) 1098, 1675, 1687, 1823, 2085
PvuII CAGCTG 1 cut(s) 2408
RsaI GTAC 7 cut(s) 68, 846, 911, 1333, 1918, 2195, 2393
RsaNI GTAC 7 cut(s) 67, 845, 910, 1332, 1917, 2194, 2392
RseI CAYNNNNRTG 4 cut(s) 344, 1751, 1803, 2028
SapI GCTCTTC 1 cut(s) 468
SatI GCNGC 7 cut(s) 1138, 1156, 1510, 1598, 2169, 2406, 2571
Sau96I GGNCC 6 cut(s) 333, 610, 649, 1844, 2068, 2152
ScaI AGTACT 2 cut(s) 68, 846
SchI GAGTC 3 cut(s) 850, 1856, 2483
ScrFI CCNGG 8 cut(s) 404, 471, 871, 1255, 1272, 1320, 1910, 2213
SduI GDGCHC 5 cut(s) 195, 393, 604, 683, 2377
SfcI CTRYAG 1 cut(s) 705
SfoI GGCGCC 1 cut(s) 933
Sfr274I CTCGAG 1 cut(s) 1481
SinI GGWCC 6 cut(s) 333, 610, 649, 1844, 2068, 2152
SlaI CTCGAG 1 cut(s) 1481
SmiMI CAYNNNNRTG 4 cut(s) 344, 1751, 1803, 2028
SmlI CTYRAG 2 cut(s) 1481, 2467
SmoI CTYRAG 2 cut(s) 1481, 2467
SnaBI TACGTA 2 cut(s) 1231, 2197
SpeI ACTAGT 1 cut(s) 2399
SphI GCATGC 2 cut(s) 1241, 1597
SsiI CCGC 7 cut(s) 225, 852, 918, 1140, 1371, 1510, 2227
SspDI GGCGCC 1 cut(s) 931
SspMI CTAG 7 cut(s) 765, 842, 1590, 1622, 1827, 1953, 2400
StyD4I CCNGG 8 cut(s) 402, 469, 869, 1253, 1270, 1318, 1908, 2211
StyI CCWWGG 7 cut(s) 232, 764, 981, 1102, 1621, 2053, 2421
TaaI ACNGT 7 cut(s) 56, 66, 104, 649, 706, 1996, 2366
TaiI ACGT 2 cut(s) 1233, 2199
TaqI TCGA 9 cut(s) 441, 530, 537, 747, 923, 1030, 1482, 2283, 2517
TaqII GACCGA 1 cut(s) 36
TatI WGTACW 4 cut(s) 66, 844, 909, 2391
TauI GCSGC 1 cut(s) 1512
TfiI GAWTC 3 cut(s) 36, 237, 1012
TscAI CASTG 7 cut(s) 124, 481, 1048, 1126, 1607, 2133, 2371
TseFI GTSAC 3 cut(s) 136, 1016, 1859
TseI GCWGC 6 cut(s) 1137, 1155, 1597, 2168, 2405, 2570
Tsp45I GTSAC 3 cut(s) 136, 1016, 1859
TspDTI ATGAA 9 cut(s) 284, 455, 599, 1146, 1190, 1376, 1709, 1805, 2235
TspGWI ACGGA 2 cut(s) 99, 1185
TspRI CASTG 7 cut(s) 124, 481, 1048, 1126, 1607, 2133, 2371
Van91I CCANNNNNTGG 1 cut(s) 2386
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 6 cut(s) 333, 610, 649, 1844, 2068, 2152
XagI CCTNNNNNAGG 1 cut(s) 1106
XapI RAATTY 8 cut(s) 284, 532, 740, 1068, 1205, 1811, 2004, 2330
XbaI TCTAGA 1 cut(s) 1826
XceI RCATGY 5 cut(s) 1241, 1597, 1750, 2135, 2359
XcmI CCANNNNNNNNNTGG 2 cut(s) 343, 1679
XhoI CTCGAG 1 cut(s) 1481
XmaJI CCTAGG 2 cut(s) 764, 1621
XmnI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
XspI CTAG 7 cut(s) 765, 842, 1590, 1622, 1827, 1953, 2400
ZrmI AGTACT 2 cut(s) 68, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.