Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0002202

Physical Location & Seq

Genomic Coordinates Reverse (-)

4944062 .. 4946646

Gene Structure 2585 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG060700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGGCACCCCCTAATGAACCTGTTAATCCAATTCGAAATTTGTTCCATTGTGCGAGTTCGGAACTCTACTTCGACTTGTCTGATGGAAAGTTGAGTGTGAAAGGTGTTCCATTGCTCTCTGGAGTTCCAACCAATGTCACTCTCAGTCCCTTTCATTTAATCTGCCAACCTTCTTGTTCTGATGTTCCAGCTCATCTGGTCCGACGCGTCTGTGCCTTGTCCCACAAGGCAGGGTTTCTTGGGTTCAATAAGGAGGAACCTTCTGATAAGTTGACCAACTCCTTGGGTAGGTTCAGTAACAGGGATTTCCTTAGCATCTTTAGGTTCAAACCATGGTGGTCTACCATGTGGGTTGGGAAGTCTGGGTCGGATTTGCAGGTGGAGACACAATGGGTGCTCTTAGATATCCCTGAAATCAAGTCCTATGTCATAATCATACCGATCATTGAAGACAAATTCAGGTCTGCTCTTCTACCTGGTCCTGATGGACATGTCATTGTCTTTGCTGAGAGTGGTTGTACCAAAGTGAAAACTTCATATTTTGATGCCATCGCTTATGTTCATGCTTCTGATAATCCCTACACCTTGATGAAAGAGGCCTATAGTGCTCTTAGGGTTCATCTCAATACCTTTAGGCTCCTAGAAGAGAAAACAGTCCCAAGTTTAGTTGACAAGTTTGGTTGGTGCACTTGGGATGCCTTCTATTTAACTGTAGAACCTGTTGGTGTTTTCCATGGGGTCAAGGAATTTGTTGAAGCCGGTGTTCCCCCAAGGTTTATCATCATTGATGATGGCTGGCAAAGTATCAATTTTGACAGTGACGAAAACCCGAATGAGGACGCACATCTAATCCTTGGTGGGACTCAAATGACTGGTAGGCTTCACAGGTTTGAGGAGTGTGACAAGTTTAGGAACTACAAAGGAGGATCTTTGTTGGATCCTAACGCTCCTTCATATGATTCCAACAGGCAGAAGAAGTTGATAGCCAAGGCACTTGAAGTTGAGGATATTGAGAAGGCTCGTGACAGGGCCACTCAATCAGGAGTCACTGATTTGTCCGAGTTCAAAACACAATGGCAGAAGCCTAAGCAAGAGTTGGATGTGATGATTGCTGAAGAAGAAAAAATTAGTGGTTCTGCTTCTACCAATGGCTGTGGAAGCTATGGTGGAATGAAGGCTTTCACAAGCGACTTGAGAACCAAATATAAGGGTTTGGATGATATTTATGTATGGCATGCTCTTTGTGGTGCCTGGGGTGGTGTAAAGCCCGGCGCAACCCATCTCAATGCAAAGGTCACTCCTGTTAATGTCTCTTCTGGACTCGACGGGTTGATGACTGATCTTGCTGTGGTGAAACTCATAGAGGGTGGCATTGGACTTGTTCATCCTGATCAAGCTGACGATTTCTTCAATTCTATGCACTCCTATCTTTCTGAAGTTGGTATAACAGGAGTCAAAGTGGACGTTATTCATAGTCTTGAGTTTTTAGGTGAGGAACACGGAGGCCGTCTGGAGCTTCCAAAGGCATATTACAAGGGGCTTACTTCTTCCCTTGTAAATAACTTCAATGGGACTGGACTTATTTCTAGCATGCAACAATGCAATGACTTCTTCTTCCTTGGGACTAAGCAAATCTCTATTGGAAGAGTTGGTGATGACTTTTGGTTCCGTGGTGGAGATGGAGACCCTATGGCAGTTTATTGGTTACAAGGAGTTCATATGATCCATTGTGCTTACAACAGCATGTGGATGAGTCAGTTCATCCAACCTGATTGGGATATGTTCCAATCAGAACATCCATGTGGCCAATTTCATGCAGCATCGCGGGCAATATGTGGAGGTCCGGTGTATGTGAGTGACTCGCTTGATTGTCATGATTTTGATCTCATCAAGAAGTTTGCTTACCCAGATGGAACCATTCCCGAGTGCCAGCATTTTGCCCTTCCAACTAGGGACTGTCTATTCAAAAACCCTTTGAATGACAGCAAGTCCGCTCTCAAAATTTGGAACTTGAACAAGTATGCTGGTGTCATTGGAGCTTTCAACTGCCAAGGGGCTGGTTGGAATTCAAAAGAGCAAAAGATCAGAGGCTATCCCGAATGCTATGAGGAAATCCATTGTTCAGTGCATGTTTCTGAAATTGAATGGGACCAAAAGAAAGAAGCAGCCAATTTGGGCAAGGCTGAACAGTATGTGGTCTATCTCAACCAGACTGATGAACTCCTCCTCATGACCCCCAAGTCTGATCCAATTCAAATGGTAGAATATTCTGTAAATATTTACTTTCCTTGTACGTTTAGATTACATCTCTCTGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167

KEGG Pathways

Metabolic & Signaling

ko00052 map00052

Pfam Domains

Protein Families

Raffinose_syn

Protein Analysis

772

Amino Acids

86.31

Weight (kDa)

5.5

Isoelectric Point (pI)

34.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Raffinose_syn	PF05691	24 - 753	8.6e-273	Raffinose synthase or seed imbibition protein Sip1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002202)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01970 AT4G01970
fragaria_vesca	FvH4_1g27000 FvH4_7g13680
malus_domestica	MD09G1285100.v1.1 MD15G1191100.v1.1
prunus_persica	Prupe.3G008900_v2.0.a1 Prupe.7G226100_v2.0.a1
pyrus_communis	pycom09g19250
rosa_chinensis	RchiOBHm_Chr1g0353751 RchiOBHm_Chr2g0091201 RchiOBHm_Chr3g0487291 RchiOBHm_Chr3g0487311
rosa_laevigata	RLG00000016191 RLG00000023016 RLG00000023018 RLG00000028260
rosa_multiflora	Rmu_co8130058.1_g000001 Rmu_sc0002270.1_g000014 Rmu_sc0021704.1_g000002 Rmu_sc0033684.1_g000007
rosa_roxburghii	Rroxscaffold_2G00150390 Rroxscaffold_4G00301680 Rroxscaffold_6G00394920 Rroxscaffold_6G00394970
rosa_rugosa	Rorug01G0237700 Rorug02G0014400 Rorug03G0232800
rosa_samantha	Rh1AG249700 Rh1BG219900 Rh1CG232300 Rh1DG246900 Rh2BG058900 Rh2CG060700 Rh3AG280600 Rh3AG280800 Rh3BG316500 Rh3CG314700 Rh3DG312500
rosa_wichuraiana	Rw1G021790 Rw2G004970 Rw3G024860 Rw3G024890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	367
AasI	GACNNNNNNGTC	1 cut(s)	2239
Acc36I	ACCTGC	1 cut(s)	367
AccB1I	GGYRCC	2 cut(s)	4, 1247
AccBSI	CCGCTC	1 cut(s)	1994
AccI	GTMKAC	1 cut(s)	341
AccII	CGCG	2 cut(s)	207, 1825
AciI	CCGC	2 cut(s)	1825, 1992
AclWI	GGATC	5 cut(s)	932, 934, 945, 1717, 2240
AcoI	YGGCCR	1 cut(s)	1804
AcsI	RAATTY	5 cut(s)	37, 455, 746, 2001, 2065
AcuI	CTGAAG	2 cut(s)	1134, 1455
AfaI	GTAC	2 cut(s)	520, 2293
AfiI	CCNNNNNNNGG	2 cut(s)	288, 835
AflIII	ACRYGT	2 cut(s)	205, 490
AhdI	GACNNNNNGTC	1 cut(s)	1987
AjnI	CCWGG	2 cut(s)	475, 1250
AluBI	AGCT	5 cut(s)	191, 1161, 1397, 1516, 2039
AluI	AGCT	5 cut(s)	191, 1161, 1397, 1516, 2039
Alw21I	GWGCWC	3 cut(s)	399, 610, 689
Alw26I	GTCTC	3 cut(s)	377, 1315, 1677
Alw44I	GTGCAC	1 cut(s)	685
AlwI	GGATC	5 cut(s)	932, 934, 945, 1717, 2240
Ama87I	CYCGRG	1 cut(s)	1922
AoxI	GGCC	4 cut(s)	597, 1029, 1504, 1804
ApaLI	GTGCAC	1 cut(s)	685
ApeKI	GCWGC	2 cut(s)	1817, 2165
ApoI	RAATTY	5 cut(s)	37, 455, 746, 2001, 2065
Asp700I	GAANNNNTTC	1 cut(s)	1178
AspLEI	GCGC	1 cut(s)	1274
AspS9I	GGNCC	5 cut(s)	199, 479, 1029, 1841, 2149
AsuC2I	CCSGG	1 cut(s)	1269
AsuHPI	GGTGA	3 cut(s)	1363, 1502, 1664
AsuII	TTCGAA	1 cut(s)	34
AvaI	CYCGRG	1 cut(s)	1922
AvaII	GGWCC	4 cut(s)	199, 479, 1841, 2149
BaeGI	GKGCMC	1 cut(s)	689
BalI	TGGCCA	1 cut(s)	1806
BamHI	GGATCC	1 cut(s)	937
BanI	GGYRCC	2 cut(s)	4, 1247
BarI	GAAGNNNNNNTAC	2 cut(s)	1886, 1918
BauI	CACGAG	1 cut(s)	1020
BbsI	GAAGAC	1 cut(s)	456
Bbv12I	GWGCWC	3 cut(s)	399, 610, 689
BbvI	GCAGC	2 cut(s)	1829, 2177
BccI	CCATC	7 cut(s)	77, 479, 557, 785, 1287, 1673, 1904
BceAI	ACGGC	1 cut(s)	1491
BciT130I	CCWGG	2 cut(s)	477, 1252
BclI	TGATCA	1 cut(s)	1390
BcnI	CCSGG	1 cut(s)	1269
BcoDI	GTCTC	3 cut(s)	377, 1315, 1677
BfaI	CTAG	3 cut(s)	641, 1587, 1950
BfmI	CTRYAG	2 cut(s)	601, 711
BfuAI	ACCTGC	1 cut(s)	367
BisI	GCNGC	2 cut(s)	1818, 2166
BlsI	GCNGC	2 cut(s)	1819, 2167
Bme1390I	CCNGG	3 cut(s)	477, 1252, 1269
Bme18I	GGWCC	4 cut(s)	199, 479, 1841, 2149
BmeRI	GACNNNNNGTC	1 cut(s)	1987
BmeT110I	CYCGRG	1 cut(s)	1922
BmgT120I	GGNCC	5 cut(s)	199, 479, 1029, 1841, 2149
BmiI	GGNNCC	8 cut(s)	6, 258, 638, 939, 1249, 1667, 1915, 2150
BmrFI	CCNGG	3 cut(s)	477, 1252, 1269
BmsI	GCATC	4 cut(s)	324, 535, 685, 1829
BpiI	GAAGAC	1 cut(s)	456
BpmI	CTGGAG	2 cut(s)	141, 1532
Bpu10I	CCTNAGC	2 cut(s)	311, 1086
Bpu14I	TTCGAA	1 cut(s)	34
BpuEI	CTTGAG	2 cut(s)	1213, 1499
BpuMI	CCSGG	1 cut(s)	1269
BsaBI	GATNNNNATC	1 cut(s)	1881
BsaI	GGTCTC	1 cut(s)	1677
BsaWI	WCCGGW	1 cut(s)	1843
BsaXI	ACNNNNNCTCC	4 cut(s)	1443, 1473, 1830, 1860
Bsc4I	CCNNNNNNNGG	2 cut(s)	288, 835
Bse118I	RCCGGY	1 cut(s)	758
Bse1I	ACTGG	2 cut(s)	877, 1579
Bse3DI	GCAATG	2 cut(s)	110, 1609
Bse8I	GATNNNNATC	1 cut(s)	1881
BseBI	CCWGG	2 cut(s)	477, 1252
BseGI	GGATG	7 cut(s)	700, 1105, 1222, 1384, 1755, 1761, 1795
BseJI	GATNNNNATC	1 cut(s)	1881
BseLI	CCNNNNNNNGG	2 cut(s)	288, 835
BseMI	GCAATG	2 cut(s)	110, 1609
BseMII	CTCAG	2 cut(s)	157, 498
BseNI	ACTGG	2 cut(s)	877, 1579
BseRI	GAGGAG	3 cut(s)	908, 2213, 2216
BseSI	GKGCMC	1 cut(s)	689
BseXI	GCAGC	2 cut(s)	1829, 2177
Bsh1236I	CGCG	2 cut(s)	207, 1825
BshFI	GGCC	4 cut(s)	599, 1031, 1506, 1806
BshNI	GGYRCC	2 cut(s)	4, 1247
BsiHKAI	GWGCWC	3 cut(s)	399, 610, 689
BsiHKCI	CYCGRG	1 cut(s)	1922
BsiSI	CCGG	3 cut(s)	759, 1269, 1844
BslFI	GGGAC	8 cut(s)	132, 205, 641, 874, 1585, 1636, 1967, 2162
BslI	CCNNNNNNNGG	2 cut(s)	288, 835
BsmAI	GTCTC	3 cut(s)	377, 1315, 1677
BsmFI	GGGAC	8 cut(s)	132, 205, 641, 874, 1585, 1636, 1967, 2162
BsmI	GAATGC	1 cut(s)	2105
BsnI	GGCC	4 cut(s)	599, 1031, 1506, 1806
Bso31I	GGTCTC	1 cut(s)	1677
BsoBI	CYCGRG	1 cut(s)	1922
Bsp119I	TTCGAA	1 cut(s)	34
Bsp1286I	GDGCHC	3 cut(s)	399, 610, 689
Bsp143I	GATC	9 cut(s)	441, 926, 937, 1339, 1390, 1722, 1882, 2082, 2245
Bsp19I	CCATGG	2 cut(s)	332, 733
BspACI	CCGC	2 cut(s)	1825, 1992
BspANI	GGCC	4 cut(s)	599, 1031, 1506, 1806
BspCNI	CTCAG	2 cut(s)	156, 499
BspFNI	CGCG	2 cut(s)	207, 1825
BspHI	TCATGA	2 cut(s)	1873, 2229
BspLI	GGNNCC	8 cut(s)	6, 258, 638, 939, 1249, 1667, 1915, 2150
BspMI	ACCTGC	1 cut(s)	367
BspPI	GGATC	5 cut(s)	932, 934, 945, 1717, 2240
BspQI	GCTCTTC	1 cut(s)	474
BspT104I	TTCGAA	1 cut(s)	34
BspT107I	GGYRCC	2 cut(s)	4, 1247
BspTNI	GGTCTC	1 cut(s)	1677
BsrBI	CCGCTC	1 cut(s)	1994
BsrDI	GCAATG	2 cut(s)	110, 1609
BsrFI	RCCGGY	1 cut(s)	758
BsrI	ACTGG	2 cut(s)	877, 1579
BssAI	RCCGGY	1 cut(s)	758
BssMI	GATC	9 cut(s)	441, 926, 937, 1339, 1390, 1722, 1882, 2082, 2245
BssSI	CACGAG	1 cut(s)	1020
BssT1I	CCWWGG	8 cut(s)	282, 332, 733, 770, 853, 987, 1618, 2050
Bst2BI	CACGAG	1 cut(s)	1020
Bst2UI	CCWGG	2 cut(s)	477, 1252
Bst4CI	ACNGT	5 cut(s)	655, 712, 818, 1958, 2190
Bst6I	CTCTTC	4 cut(s)	474, 639, 1318, 1639
BstBI	TTCGAA	1 cut(s)	34
BstC8I	GCNNGC	5 cut(s)	797, 1236, 1592, 1827, 1931
BstDEI	CTNAG	7 cut(s)	143, 311, 400, 507, 611, 1086, 1626
BstDSI	CCRYGG	3 cut(s)	332, 733, 1669
BstENI	CCTNNNNNAGG	1 cut(s)	286
BstF5I	GGATG	7 cut(s)	700, 1105, 1222, 1384, 1755, 1761, 1795
BstFNI	CGCG	2 cut(s)	207, 1825
BstHHI	GCGC	1 cut(s)	1274
BstKTI	GATC	9 cut(s)	444, 929, 940, 1342, 1393, 1725, 1885, 2085, 2248
BstMAI	GTCTC	3 cut(s)	377, 1315, 1677
BstMBI	GATC	9 cut(s)	441, 926, 937, 1339, 1390, 1722, 1882, 2082, 2245
BstMWI	GCNNNNNNNGC	3 cut(s)	605, 1158, 1826
BstNI	CCWGG	2 cut(s)	477, 1252
BstNSI	RCATGY	5 cut(s)	494, 1238, 1594, 1747, 2132
BstSCI	CCNGG	3 cut(s)	475, 1250, 1267
BstSFI	CTRYAG	2 cut(s)	601, 711
BstSLI	GKGCMC	1 cut(s)	689
BstUI	CGCG	2 cut(s)	207, 1825
BstV1I	GCAGC	2 cut(s)	1829, 2177
BstV2I	GAAGAC	1 cut(s)	456
BstX2I	RGATCY	2 cut(s)	926, 937
BstXI	CCANNNNNNTGG	2 cut(s)	283, 2057
BstYI	RGATCY	2 cut(s)	926, 937
BsuRI	GGCC	4 cut(s)	599, 1031, 1506, 1806
BtgI	CCRYGG	3 cut(s)	332, 733, 1669
BtgZI	GCGATG	2 cut(s)	535, 1806
BtsCI	GGATG	7 cut(s)	700, 1105, 1222, 1384, 1755, 1761, 1795
BtsIMutI	CAGTG	3 cut(s)	823, 1047, 2130
BveI	ACCTGC	1 cut(s)	367
Cac8I	GCNNGC	5 cut(s)	797, 1236, 1592, 1827, 1931
CciI	TCATGA	2 cut(s)	1873, 2229
CfoI	GCGC	1 cut(s)	1274
Cfr10I	RCCGGY	1 cut(s)	758
Cfr13I	GGNCC	5 cut(s)	199, 479, 1029, 1841, 2149
CseI	GACGC	3 cut(s)	196, 213, 848
CsiI	ACCWGGT	1 cut(s)	475
Csp6I	GTAC	2 cut(s)	519, 2292
CspCI	CAANNNNNGTGG	2 cut(s)	1135, 1170
CviQI	GTAC	2 cut(s)	519, 2292
DdeI	CTNAG	7 cut(s)	143, 311, 400, 507, 611, 1086, 1626
DpnI	GATC	9 cut(s)	443, 928, 939, 1341, 1392, 1724, 1884, 2084, 2247
DpnII	GATC	9 cut(s)	441, 926, 937, 1339, 1390, 1722, 1882, 2082, 2245
DrdI	GACNNNNNNGTC	1 cut(s)	2239
DriI	GACNNNNNGTC	1 cut(s)	1987
DseDI	GACNNNNNNGTC	1 cut(s)	2239
EaeI	YGGCCR	1 cut(s)	1804
Eam1104I	CTCTTC	4 cut(s)	474, 639, 1318, 1639
Eam1105I	GACNNNNNGTC	1 cut(s)	1987
EarI	CTCTTC	4 cut(s)	474, 639, 1318, 1639
Eco130I	CCWWGG	8 cut(s)	282, 332, 733, 770, 853, 987, 1618, 2050
Eco147I	AGGCCT	1 cut(s)	599
Eco31I	GGTCTC	1 cut(s)	1677
Eco32I	GATATC	1 cut(s)	406
Eco47I	GGWCC	4 cut(s)	199, 479, 1841, 2149
Eco57I	CTGAAG	2 cut(s)	1134, 1455
Eco88I	CYCGRG	1 cut(s)	1922
EcoNI	CCTNNNNNAGG	1 cut(s)	286
EcoRI	GAATTC	1 cut(s)	2065
EcoRII	CCWGG	2 cut(s)	475, 1250
EcoRV	GATATC	1 cut(s)	406
EcoT14I	CCWWGG	8 cut(s)	282, 332, 733, 770, 853, 987, 1618, 2050
ErhI	CCWWGG	8 cut(s)	282, 332, 733, 770, 853, 987, 1618, 2050
FaqI	GGGAC	8 cut(s)	132, 205, 641, 874, 1585, 1636, 1967, 2162
FauI	CCCGC	1 cut(s)	1818
FauNDI	CATATG	2 cut(s)	955, 1719
FbaI	TGATCA	1 cut(s)	1390
FblI	GTMKAC	1 cut(s)	341
Fnu4HI	GCNGC	2 cut(s)	1818, 2166
FokI	GGATG	7 cut(s)	707, 1112, 1229, 1371, 1748, 1762, 1782
Fsp4HI	GCNGC	2 cut(s)	1818, 2166
FspBI	CTAG	3 cut(s)	641, 1587, 1950
GlaI	GCGC	1 cut(s)	1273
GluI	GCNGC	2 cut(s)	1818, 2166
GsuI	CTGGAG	2 cut(s)	141, 1532
HaeIII	GGCC	4 cut(s)	599, 1031, 1506, 1806
HapII	CCGG	3 cut(s)	759, 1269, 1844
HgaI	GACGC	3 cut(s)	196, 213, 848
HhaI	GCGC	1 cut(s)	1274
Hin6I	GCGC	1 cut(s)	1272
HinP1I	GCGC	1 cut(s)	1272
HincII	GTYRAC	2 cut(s)	273, 670
HindII	GTYRAC	2 cut(s)	273, 670
HinfI	GANTC	7 cut(s)	862, 959, 1044, 1320, 1452, 1753, 1859
HpaII	CCGG	3 cut(s)	759, 1269, 1844
HphI	GGTGA	3 cut(s)	1363, 1502, 1664
Hpy166II	GTNNAC	5 cut(s)	273, 342, 670, 687, 1462
Hpy8I	GTNNAC	5 cut(s)	273, 342, 670, 687, 1462
Hpy99I	CGWCG	2 cut(s)	207, 1328
HpyAV	CCTTC	7 cut(s)	180, 270, 709, 960, 1009, 1168, 1952
HpyCH4III	ACNGT	5 cut(s)	655, 712, 818, 1958, 2190
HpyCH4IV	ACGT	2 cut(s)	1464, 2294
HpyCH4V	TGCA	8 cut(s)	376, 687, 1289, 1420, 1594, 1602, 1817, 2128
HpyF10VI	GCNNNNNNNGC	3 cut(s)	605, 1158, 1826
HpyF3I	CTNAG	7 cut(s)	143, 311, 400, 507, 611, 1086, 1626
HpySE526I	ACGT	2 cut(s)	1464, 2294
HspAI	GCGC	1 cut(s)	1272
Ksp22I	TGATCA	1 cut(s)	1390
Kzo9I	GATC	9 cut(s)	441, 926, 937, 1339, 1390, 1722, 1882, 2082, 2245
LguI	GCTCTTC	1 cut(s)	474
LmnI	GCTCC	4 cut(s)	642, 952, 1513, 2036
Lsp1109I	GCAGC	2 cut(s)	1829, 2177
LweI	GCATC	4 cut(s)	324, 535, 685, 1829
MabI	ACCWGGT	1 cut(s)	475
MaeI	CTAG	3 cut(s)	641, 1587, 1950
MaeII	ACGT	2 cut(s)	1464, 2294
MaeIII	GTNAC	9 cut(s)	136, 296, 818, 899, 1022, 1045, 1294, 1704, 1856
MalI	GATC	9 cut(s)	443, 928, 939, 1341, 1392, 1724, 1884, 2084, 2247
MbiI	CCGCTC	1 cut(s)	1994
MboI	GATC	9 cut(s)	441, 926, 937, 1339, 1390, 1722, 1882, 2082, 2245
MflI	RGATCY	2 cut(s)	926, 937
MhlI	GDGCHC	3 cut(s)	399, 610, 689
MlsI	TGGCCA	1 cut(s)	1806
MluI	ACGCGT	1 cut(s)	205
MluNI	TGGCCA	1 cut(s)	1806
MlyI	GAGTC	6 cut(s)	856, 1053, 1314, 1461, 1762, 1853
MmeI	TCCRAC	9 cut(s)	152, 226, 348, 915, 987, 1077, 1789, 1970, 2042
Mox20I	TGGCCA	1 cut(s)	1806
MroXI	GAANNNNTTC	1 cut(s)	1178
MscI	TGGCCA	1 cut(s)	1806
MseI	TTAA	4 cut(s)	24, 158, 707, 1305
MslI	CAYNNNNRTG	4 cut(s)	587, 1284, 1748, 1800
Msp20I	TGGCCA	1 cut(s)	1806
MspI	CCGG	3 cut(s)	759, 1269, 1844
MspR9I	CCNGG	3 cut(s)	477, 1252, 1269
Mva1269I	GAATGC	1 cut(s)	2105
MvaI	CCWGG	2 cut(s)	477, 1252
MvnI	CGCG	2 cut(s)	207, 1825
MwoI	GCNNNNNNNGC	3 cut(s)	605, 1158, 1826
NciI	CCSGG	1 cut(s)	1269
NcoI	CCATGG	2 cut(s)	332, 733
NdeI	CATATG	2 cut(s)	955, 1719
NdeII	GATC	9 cut(s)	441, 926, 937, 1339, 1390, 1722, 1882, 2082, 2245
NlaIV	GGNNCC	8 cut(s)	6, 258, 638, 939, 1249, 1667, 1915, 2150
NmuCI	GTSAC	7 cut(s)	136, 818, 899, 1022, 1045, 1294, 1856
NspI	RCATGY	5 cut(s)	494, 1238, 1594, 1747, 2132
NspV	TTCGAA	1 cut(s)	34
PaeI	GCATGC	2 cut(s)	1238, 1594
PagI	TCATGA	2 cut(s)	1873, 2229
PaqCI	CACCTGC	1 cut(s)	367
PceI	AGGCCT	1 cut(s)	599
PciI	ACATGT	1 cut(s)	490
PciSI	GCTCTTC	1 cut(s)	474
PctI	GAATGC	1 cut(s)	2105
PdmI	GAANNNNTTC	1 cut(s)	1178
PfeI	GAWTC	1 cut(s)	959
PkrI	GCNGC	2 cut(s)	1819, 2167
PleI	GAGTC	6 cut(s)	856, 1052, 1314, 1460, 1761, 1853
PpsI	GAGTC	6 cut(s)	856, 1052, 1314, 1460, 1761, 1853
PscI	ACATGT	1 cut(s)	490
Psp6I	CCWGG	2 cut(s)	475, 1250
PspGI	CCWGG	2 cut(s)	475, 1250
PspN4I	GGNNCC	8 cut(s)	6, 258, 638, 939, 1249, 1667, 1915, 2150
PspPI	GGNCC	5 cut(s)	199, 479, 1029, 1841, 2149
PsuI	RGATCY	2 cut(s)	926, 937
RsaI	GTAC	2 cut(s)	520, 2293
RsaNI	GTAC	2 cut(s)	519, 2292
RseI	CAYNNNNRTG	4 cut(s)	587, 1284, 1748, 1800
SapI	GCTCTTC	1 cut(s)	474
SaqAI	TTAA	4 cut(s)	24, 158, 707, 1305
SatI	GCNGC	2 cut(s)	1818, 2166
Sau3AI	GATC	9 cut(s)	441, 926, 937, 1339, 1390, 1722, 1882, 2082, 2245
Sau96I	GGNCC	5 cut(s)	199, 479, 1029, 1841, 2149
SchI	GAGTC	6 cut(s)	856, 1053, 1314, 1461, 1762, 1853
ScrFI	CCNGG	3 cut(s)	477, 1252, 1269
SduI	GDGCHC	3 cut(s)	399, 610, 689
SexAI	ACCWGGT	1 cut(s)	475
SfaNI	GCATC	4 cut(s)	324, 535, 685, 1829
SfcI	CTRYAG	2 cut(s)	601, 711
SfuI	TTCGAA	1 cut(s)	34
SinI	GGWCC	4 cut(s)	199, 479, 1841, 2149
SmiMI	CAYNNNNRTG	4 cut(s)	587, 1284, 1748, 1800
SmlI	CTYRAG	2 cut(s)	1192, 1478
SmoI	CTYRAG	2 cut(s)	1192, 1478
SphI	GCATGC	2 cut(s)	1238, 1594
SseBI	AGGCCT	1 cut(s)	599
SsiI	CCGC	2 cut(s)	1825, 1992
SspI	AATATT	2 cut(s)	2267, 2278
SspMI	CTAG	3 cut(s)	641, 1587, 1950
StuI	AGGCCT	1 cut(s)	599
StyD4I	CCNGG	3 cut(s)	475, 1250, 1267
StyI	CCWWGG	8 cut(s)	282, 332, 733, 770, 853, 987, 1618, 2050
TaaI	ACNGT	5 cut(s)	655, 712, 818, 1958, 2190
TaiI	ACGT	2 cut(s)	1467, 2297
TaqI	TCGA	3 cut(s)	34, 72, 1323
TfiI	GAWTC	1 cut(s)	959
Tru1I	TTAA	4 cut(s)	24, 158, 707, 1305
Tru9I	TTAA	4 cut(s)	24, 158, 707, 1305
TscAI	CASTG	3 cut(s)	823, 1054, 2130
TseFI	GTSAC	7 cut(s)	136, 818, 899, 1022, 1045, 1294, 1856
TseI	GCWGC	2 cut(s)	1817, 2165
Tsp45I	GTSAC	7 cut(s)	136, 818, 899, 1022, 1045, 1294, 1856
TspGWI	ACGGA	2 cut(s)	1515, 1658
TspRI	CASTG	3 cut(s)	823, 1054, 2130
VneI	GTGCAC	1 cut(s)	685
VpaK11BI	GGWCC	4 cut(s)	199, 479, 1841, 2149
XagI	CCTNNNNNAGG	1 cut(s)	286
XapI	RAATTY	5 cut(s)	37, 455, 746, 2001, 2065
XceI	RCATGY	5 cut(s)	494, 1238, 1594, 1747, 2132
XmiI	GTMKAC	1 cut(s)	341
XmnI	GAANNNNTTC	1 cut(s)	1178
XspI	CTAG	3 cut(s)	641, 1587, 1950

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.