MD15G1191100.v1.1

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002202
Physical Location & Seq
Reverse (-)
15025285 .. 15026079
795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1191100.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGCGCCTCCTAATCTTCCTGTTAATCCGATCAACGATGTTTCTCTGCCTGACATCATAGAAGAATATTTCGACTTGTCGGATGGAAAGCTTAGTGTTAGAGGTGTTCCACTGCTTGATGAAGTTCCAAACAATGTCACTTTTAGTCCCTTTAATTCAATCTGCCAGCCTTGTGACGATGTTCCACTTCCCCTGCTCAACCGTGTCGGTGCCTTGTCACACAAGGAAGGGTTCCTGGGGTTCAAAGCGGATGTGCCTTCGGATAGGTTGAAGAATTCCTTGGGAAGATCCAGTGACAGGGATTTTCTCAGCATTTTTCAGTTCAAGACATGGTGGTCTACCATGTGGGTTGGAAACTCCGGTTCGAATTTACAGAAGGAAACACAATGGGTGCTTTTTGACGTCCCTGAGATAAAATCATATGTGATCATCATACCGATTATTGATGGATCTTTTAGGTCTGCTCTTCAACCCGGTAATGATGGACATGTCGTGATCTGTGCCGAAAGCGGGTCTACGCAAGTGAAAGTGTCGAATTTCGATGTTATTGCTTATGTTCATGCTTCTGACAATCCTTACAACTTGATGAAAGAGGCCTTTAGTGCTATTAGGGTTCACCTTAATACCTTCAGGCTGTTGGAAGAGAAATTAGTTCCGAATCTGGTTGACAAATTCGGTTGGTGCACTTGGGATGCCTTCTACTTAACGGTTGAACCTGCTGGAATCTGGCACGGTATAAATGAGTTCACTGAAGCCGGTGTGTCTTCTCGCTTTATCATCGTTGATGATGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

265

Amino Acids

29.44

Weight (kDa)

4.71

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 264 1.4e-83 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 334
AatII GACGTC 1 cut(s) 405
Acc36I ACCTGC 1 cut(s) 724
AccB1I GGYRCC 2 cut(s) 4, 209
AccI GTMKAC 2 cut(s) 338, 515
AciI CCGC 2 cut(s) 248, 510
AclWI GGATC 2 cut(s) 282, 457
AcsI RAATTY 4 cut(s) 274, 367, 535, 671
AcuI CTGAAG 2 cut(s) 613, 771
AcyI GRCGYC 2 cut(s) 5, 402
AfiI CCNNNNNNNGG 2 cut(s) 297, 510
AflIII ACRYGT 1 cut(s) 487
AgsI TTSAA 6 cut(s) 159, 244, 271, 325, 470, 713
AjnI CCWGG 1 cut(s) 234
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 1 cut(s) 91
AluI AGCT 1 cut(s) 91
Alw21I GWGCWC 1 cut(s) 686
Alw44I GTGCAC 1 cut(s) 682
AlwI GGATC 2 cut(s) 282, 457
AoxI GGCC 1 cut(s) 594
ApaLI GTGCAC 1 cut(s) 682
ApoI RAATTY 4 cut(s) 274, 367, 535, 671
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 1 cut(s) 608
AsuII TTCGAA 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 686
BanI GGYRCC 2 cut(s) 4, 209
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 1 cut(s) 686
BccI CCATC 4 cut(s) 77, 440, 476, 782
BcgI CGANNNNNNTGC 2 cut(s) 530, 564
BciT130I CCWGG 1 cut(s) 236
BclI TGATCA 1 cut(s) 426
BcnI CCSGG 1 cut(s) 474
BfaI CTAG 1 cut(s) 793
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 724
Bme1390I CCNGG 2 cut(s) 236, 474
BmiI GGNNCC 3 cut(s) 6, 211, 233
BmrFI CCNGG 2 cut(s) 236, 474
BmsI GCATC 1 cut(s) 682
BpiI GAAGAC 1 cut(s) 756
Bpu14I TTCGAA 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 474
BsaHI GRCGYC 2 cut(s) 5, 402
BsaJI CCNNGG 2 cut(s) 235, 279
BsaWI WCCGGW 1 cut(s) 359
Bsc4I CCNNNNNNNGG 2 cut(s) 297, 510
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 1 cut(s) 291
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 2 cut(s) 235, 279
BseGI GGATG 3 cut(s) 88, 256, 697
BseLI CCNNNNNNNGG 2 cut(s) 297, 510
BseMII CTCAG 2 cut(s) 322, 399
BseNI ACTGG 1 cut(s) 291
BseSI GKGCMC 1 cut(s) 686
BshFI GGCC 1 cut(s) 596
BshNI GGYRCC 2 cut(s) 4, 209
BsiHKAI GWGCWC 1 cut(s) 686
BsiSI CCGG 3 cut(s) 360, 474, 756
BslFI GGGAC 2 cut(s) 132, 389
BslI CCNNNNNNNGG 2 cut(s) 297, 510
BsmFI GGGAC 2 cut(s) 132, 389
BsnI GGCC 1 cut(s) 596
Bsp119I TTCGAA 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 686
Bsp143I GATC 5 cut(s) 30, 287, 426, 449, 495
BspACI CCGC 2 cut(s) 248, 510
BspANI GGCC 1 cut(s) 596
BspCNI CTCAG 2 cut(s) 321, 400
BspLI GGNNCC 3 cut(s) 6, 211, 233
BspMI ACCTGC 1 cut(s) 724
BspPI GGATC 2 cut(s) 282, 457
BspQI GCTCTTC 1 cut(s) 471
BspT104I TTCGAA 1 cut(s) 365
BspT107I GGYRCC 2 cut(s) 4, 209
BsrFI RCCGGY 1 cut(s) 755
BsrI ACTGG 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 2 cut(s) 235, 279
BssMI GATC 5 cut(s) 30, 287, 426, 449, 495
BssNI GRCGYC 2 cut(s) 5, 402
BssT1I CCWWGG 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 3 cut(s) 203, 709, 734
Bst6I CTCTTC 2 cut(s) 471, 636
BstACI GRCGYC 2 cut(s) 5, 402
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 1 cut(s) 167
BstDEI CTNAG 3 cut(s) 92, 308, 408
BstF5I GGATG 3 cut(s) 88, 256, 697
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 5 cut(s) 33, 290, 429, 452, 498
BstMBI GATC 5 cut(s) 30, 287, 426, 449, 495
BstMWI GCNNNNNNNGC 1 cut(s) 602
BstNI CCWGG 1 cut(s) 236
BstNSI RCATGY 1 cut(s) 491
BstSCI CCNGG 2 cut(s) 234, 472
BstSLI GKGCMC 1 cut(s) 686
BstV2I GAAGAC 1 cut(s) 756
BstX2I RGATCY 2 cut(s) 287, 449
BstYI RGATCY 2 cut(s) 287, 449
BsuRI GGCC 1 cut(s) 596
BtsCI GGATG 3 cut(s) 88, 256, 697
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 3 cut(s) 110, 298, 747
BveI ACCTGC 1 cut(s) 724
Cac8I GCNNGC 1 cut(s) 167
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 755
CviAII CATG 4 cut(s) 330, 343, 488, 560
CviJI RGCY 6 cut(s) 91, 169, 596, 634, 755, 792
CviKI_1 RGCY 6 cut(s) 91, 169, 596, 634, 755, 792
DdeI CTNAG 3 cut(s) 92, 308, 408
DinI GGCGCC 1 cut(s) 6
DpnI GATC 5 cut(s) 32, 289, 428, 451, 497
DpnII GATC 5 cut(s) 30, 287, 426, 449, 495
DrdI GACNNNNNNGTC 1 cut(s) 334
DseDI GACNNNNNNGTC 1 cut(s) 334
Eam1104I CTCTTC 2 cut(s) 471, 636
EarI CTCTTC 2 cut(s) 471, 636
Eco130I CCWWGG 1 cut(s) 279
Eco147I AGGCCT 1 cut(s) 596
Eco57I CTGAAG 2 cut(s) 613, 771
EcoRI GAATTC 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 279
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 4 cut(s) 333, 346, 491, 563
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 2 cut(s) 132, 389
FatI CATG 4 cut(s) 329, 342, 487, 559
FauI CCCGC 1 cut(s) 503
FauNDI CATATG 1 cut(s) 421
FbaI TGATCA 1 cut(s) 426
FblI GTMKAC 2 cut(s) 338, 515
FokI GGATG 3 cut(s) 95, 263, 704
FspBI CTAG 1 cut(s) 793
GlaI GCGC 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 596
HapII CCGG 3 cut(s) 360, 474, 756
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 2 cut(s) 5, 402
Hin1II CATG 4 cut(s) 333, 346, 491, 563
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 658, 723
HpaII CCGG 3 cut(s) 360, 474, 756
HphI GGTGA 1 cut(s) 608
Hpy166II GTNNAC 6 cut(s) 339, 516, 616, 667, 684, 747
Hpy188I TCNGA 5 cut(s) 30, 82, 262, 568, 657
Hpy188III TCNNGA 2 cut(s) 325, 493
Hpy8I GTNNAC 6 cut(s) 339, 516, 616, 667, 684, 747
HpyAV CCTTC 5 cut(s) 221, 267, 370, 637, 706
HpyCH4III ACNGT 3 cut(s) 203, 709, 734
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 1 cut(s) 684
HpyF10VI GCNNNNNNNGC 1 cut(s) 602
HpyF3I CTNAG 3 cut(s) 92, 308, 408
HpySE526I ACGT 1 cut(s) 402
Hsp92I GRCGYC 2 cut(s) 5, 402
Hsp92II CATG 4 cut(s) 333, 346, 491, 563
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 426
Kzo9I GATC 5 cut(s) 30, 287, 426, 449, 495
LguI GCTCTTC 1 cut(s) 471
LweI GCATC 1 cut(s) 682
MaeI CTAG 1 cut(s) 793
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 4 cut(s) 136, 173, 216, 293
MalI GATC 5 cut(s) 32, 289, 428, 451, 497
MboI GATC 5 cut(s) 30, 287, 426, 449, 495
MboII GAAGA 7 cut(s) 8, 74, 283, 297, 458, 653, 756
MflI RGATCY 2 cut(s) 287, 449
MhlI GDGCHC 1 cut(s) 686
MluCI AATT 6 cut(s) 154, 274, 367, 535, 647, 671
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 60, 331, 618
MnlI CCTC 3 cut(s) 18, 95, 586
MseI TTAA 4 cut(s) 24, 153, 621, 704
MspI CCGG 3 cut(s) 360, 474, 756
MspR9I CCNGG 2 cut(s) 236, 474
MvaI CCWGG 1 cut(s) 236
MwoI GCNNNNNNNGC 1 cut(s) 602
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 474
NdeI CATATG 1 cut(s) 421
NdeII GATC 5 cut(s) 30, 287, 426, 449, 495
NlaIII CATG 4 cut(s) 333, 346, 491, 563
NlaIV GGNNCC 3 cut(s) 6, 211, 233
NmuCI GTSAC 4 cut(s) 136, 173, 216, 293
NspI RCATGY 1 cut(s) 491
NspV TTCGAA 1 cut(s) 365
PceI AGGCCT 1 cut(s) 596
PciI ACATGT 1 cut(s) 487
PciSI GCTCTTC 1 cut(s) 471
PfeI GAWTC 2 cut(s) 658, 723
PluTI GGCGCC 1 cut(s) 8
PscI ACATGT 1 cut(s) 487
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 3 cut(s) 6, 211, 233
PsuI RGATCY 2 cut(s) 287, 449
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 4 cut(s) 24, 153, 621, 704
Sau3AI GATC 5 cut(s) 30, 287, 426, 449, 495
ScrFI CCNGG 2 cut(s) 236, 474
SduI GDGCHC 1 cut(s) 686
SetI ASST 8 cut(s) 93, 106, 269, 405, 461, 621, 629, 718
SfaNI GCATC 1 cut(s) 682
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 365
Sse9I AATT 6 cut(s) 154, 274, 367, 535, 647, 671
SseBI AGGCCT 1 cut(s) 596
SsiI CCGC 2 cut(s) 248, 510
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 68
SspMI CTAG 1 cut(s) 793
StuI AGGCCT 1 cut(s) 596
StyD4I CCNGG 2 cut(s) 234, 472
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 3 cut(s) 203, 709, 734
TaiI ACGT 1 cut(s) 405
TaqI TCGA 4 cut(s) 72, 365, 533, 540
TasI AATT 6 cut(s) 154, 274, 367, 535, 647, 671
TfiI GAWTC 2 cut(s) 658, 723
Tru1I TTAA 4 cut(s) 24, 153, 621, 704
Tru9I TTAA 4 cut(s) 24, 153, 621, 704
TscAI CASTG 3 cut(s) 117, 298, 754
TseFI GTSAC 4 cut(s) 136, 173, 216, 293
Tsp45I GTSAC 4 cut(s) 136, 173, 216, 293
TspDTI ATGAA 3 cut(s) 135, 548, 602
TspRI CASTG 3 cut(s) 117, 298, 754
VneI GTGCAC 1 cut(s) 682
XapI RAATTY 4 cut(s) 274, 367, 535, 671
XceI RCATGY 1 cut(s) 491
XmiI GTMKAC 2 cut(s) 338, 515
XspI CTAG 1 cut(s) 793
ZraI GACGTC 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.