Rroxscaffold_4G00301680

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002202
Physical Location & Seq
Forward (+)
22034732 .. 22037678
2947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301680.1

Sequence Viewer

Length: 2625 bp
ATGGCACCTCCTAATATTCCTGTTAATCCAGTCCTCAATGTTATCCGGTCCGAGAGTTCAGAAAAATACTTTGATTTGTCTGATGGAAAGTTTAATGTCAAAGGTGTTCCATTGCTCTCTGAAGTTCCAAGCAATGTCACCTTTAGTCCTTTTTCTTCAATCTGCCAATCTTCTTCTGATGTTCCAACTCATCTGCTCAGCCGTGTCAGTGCCTTGTCCCACAAGGCAGGGTTCCTTGGTTTCAACAAGGAGTCGTCTTCTGATAAGTTGATAAATTCATTGGGAAAATTCGATAACAGGGATTTCCTTAGTATCTTTAGGTTCAAAACATGGTGGTCTACTATGTGGGTTGGGAACTCTGGGTCAGATTTGCAAATGGAAACCCAATGGGTGCTCCTAAATGTTCCAGAAATCAATTCCTATGTCATAATCATACCCATCATCGATGGCAGTTTTAGATCTGCTTTTCAACCTGGTACTGATGGTCATGTCATGATCTTTGCTGAGAGTGGTTCTACCCAAGTGAAAGCATCGAAGTTTGATGCCATCGCTTATGTTCATGTGTCCGACAATCCCTACACCTTGATGAAAGAGGCCTACAGTGCTCTGAGGGTCCATCTCAATACCTTTAGGCTCCTTGAAGAAAAATCCGCACCAAATCTAGTTGATAAATTTGGTTGGTGCACTTGGGATGCCTTCTACTTAACTGTGGAACCTGTTGGTGTCTCCCATGGTGTAAATGAATTCGTTGAAGCTGGTGTACCCCCAAGGTTTCTCATCATTGATGATGGTTGGCAAAGCATTAATTTCGATGATGATGAGAACCCGAATGAGGATGCGAAGAATCTAGTCATTGGTGGGACTCAAATGACTGCTAGGCTTCACAGGCTTGAGGAGTGTGACAAGTTCAAAAACTACCAAGATGGATCTTTACTGGGTCCTCATGCACCTTCATTTGATCCAAAAAGGCCAAAGATGTTGATATCCAAGGCAATTGAATTAGAGAATGTTGAAAAGGCTCGTGATAAGGCCATTCAATCTGGAGTCACTGATTTGTCCGAGTTCCAAAAACAAATGCAGAAGCATAAACAAGAGTTGGATATGATAATTGGTGGTGGAGAAGAAGGCAGCACTACTAGTAGTGTTTCGAAAGAAGGAAGCTGTAGAAGCTGTTCTTGCAAGGCTAAAAATTATGGAATGAAGTCTTTCACATGCGACTTGAGAACCAAGTTCAAGAATTTGGATGATATTTATGTATGGCATGCTCTTTGTGGTGCCTGGGGTGGTGTTAAACCAGGTACAACTCATCTCAATTCAAAGATCATCCCCTGCAAAGTCTCTCCAGGACTAGATGGTTCCATGTCTGACCTAGCTGTGGTGAAACTAGTTGAGGGTGGGATTGGACTTGTCCATCCTGATCAAGCTGGTGATTTCTATAACTCTATGCACTCTTACCTTTCTGAAGCTGGTATCACAGGAGTCAAAGTGGATGTTATTCATACTCTTGAATATGTATCTGAGGAATTCGGAGGCCGTGTTGAGCTTGCAAAGGCTTATTACAAGGGGCTGACTTCTTCTCTTGTAAAGAACTTCAATGGAACTGGACTTATTGCTAGCATGCAACAATGCAATGATTTCTTCTTCCTTGGAACAAAACAAATCTCTATTGGAAGAGTTGGTGATGATTTTTGGTTTCAGGATCCAAATGGAGACCCCATGGGAGTTTTCTGGCTACAAGGTGTCCACATGATCCATTGTGCCTACAACAGCATGTGGATGGGTCAATTCATTCAGCCTGATTGGGATATGTTCCAGTCAGATCATCTGTGTGCCAAATTTCATGCCGGATCTAGGGCTATATGTGGAGGTCCGGTGTATATAAGTGACTCGGTTGGTGGTCACAATTTTGATCTTATTAGGCAGCTTGTTTACCCAGATGGTACCATCCCCAAGTGCCAGCATTTTGCTCTTCCAACTAGGGACTGCCTATTCAAGAACCCCTTATTTGACAGCAAGACTGCTCTCAAAATTTGGAATGTCAACAAGTTTGGTGGTGTGATGGGAGCTTTCAACTGCCAAGGGGCTGGTTGGGACCCAAAAGAAAAGAGAATCAAAGGCTACTCCCAGTGCTACATGGAAATCCTCTATTCAGTGCATGTTTCTGAAATTGAATGGGACCAAAAGATGGAAGCCGCCCATTTGGGTAAGGCCGAAGAATATGTGGTCTATCTCAACCAGGCCAATGAACTCTTCCAAATGACCCCCAAATCCGATCCCATTCAAATGGTGATCCAACCATCTTCGTTCGAGATTTTCAGCTTCGTGCCAGTACAAAAGCTCGGCTCCAACAACACCAAGTTTGCGCCGATTGGACTGACAAACATGTTCAACAGTGGAGGCACTATCCAAGAGCTGGAATATACAAGTGGCATGGAATCTTTTAGTGCAAAGATCAAGGTTAAAGGTGGAGGCAACTTCTTGGCCTACTCAAGTCAGTCTCCGAAGAAGTGTTGTTTGAATGGTGCTGTAGTGACTTTTGAGTGGTCTGCTTCTGGTAAACTGTCCCTGAATCTTCCTTGGGTTGAAGAAGCAGCTGGAATTTCTGATGTCGTTCTTGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

874

Amino Acids

96.84

Weight (kDa)

5.7

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 857 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1940
AccB1I GGYRCC 3 cut(s) 4, 1274, 1940
AccB7I CCANNNNNTGG 2 cut(s) 2185, 2413
AccI GTMKAC 1 cut(s) 338
AciI CCGC 2 cut(s) 651, 2193
AclWI GGATC 8 cut(s) 934, 953, 1694, 1707, 1744, 1855, 2267, 2284
AcsI RAATTY 9 cut(s) 274, 287, 671, 743, 1237, 1523, 1835, 2028, 2598
AcuI CTGAAG 2 cut(s) 141, 1482
AfaI GTAC 5 cut(s) 478, 762, 1300, 1942, 2331
AfiI CCNNNNNNNGG 5 cut(s) 832, 1375, 1851, 2185, 2413
AflIII ACRYGT 1 cut(s) 2382
AhlI ACTAGT 2 cut(s) 1136, 1384
AjnI CCWGG 5 cut(s) 472, 1277, 1294, 1342, 2235
AjuI GAANNNNNNNTTGG 2 cut(s) 2234, 2266
Alw21I GWGCWC 3 cut(s) 396, 607, 686
Alw26I GTCTC 4 cut(s) 730, 1342, 1704, 2502
Alw44I GTGCAC 1 cut(s) 682
AlwI GGATC 8 cut(s) 934, 953, 1694, 1707, 1744, 1855, 2267, 2284
AoxI GGCC 7 cut(s) 594, 968, 1029, 1531, 2208, 2238, 2481
ApaLI GTGCAC 1 cut(s) 682
ApeKI GCWGC 3 cut(s) 1128, 1921, 2591
ApoI RAATTY 9 cut(s) 274, 287, 671, 743, 1237, 1523, 1835, 2028, 2598
AseI ATTAAT 1 cut(s) 804
Asp700I GAANNNNTTC 2 cut(s) 1171, 1205
Asp718I GGTACC 1 cut(s) 1940
AspLEI GCGC 1 cut(s) 2365
AspS9I GGNCC 6 cut(s) 48, 613, 938, 1868, 2092, 2176
AsuHPI GGTGA 5 cut(s) 130, 1390, 1439, 1691, 2299
AsuII TTCGAA 1 cut(s) 1148
AsuNHI GCTAGC 1 cut(s) 1613
AvaII GGWCC 6 cut(s) 48, 613, 938, 1868, 2092, 2176
BaeGI GKGCMC 1 cut(s) 686
BamHI GGATCC 1 cut(s) 1699
BanI GGYRCC 3 cut(s) 4, 1274, 1940
BarI GAAGNNNNNNTAC 2 cut(s) 744, 776
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 3 cut(s) 396, 607, 686
BbvI GCAGC 3 cut(s) 1140, 1933, 2603
BceAI ACGGC 2 cut(s) 186, 1518
BcgI CGANNNNNNTGC 2 cut(s) 790, 824
BciT130I CCWGG 5 cut(s) 474, 1279, 1296, 1344, 2237
BclI TGATCA 1 cut(s) 1417
BcoDI GTCTC 4 cut(s) 730, 1342, 1704, 2502
BcuI ACTAGT 2 cut(s) 1136, 1384
BfmI CTRYAG 3 cut(s) 598, 1162, 2526
BglII AGATCT 1 cut(s) 458
BisI GCNGC 4 cut(s) 1129, 1922, 2193, 2592
BlpI GCTNAGC 1 cut(s) 197
BlsI GCNGC 4 cut(s) 1130, 1923, 2194, 2593
Bme1390I CCNGG 5 cut(s) 474, 1279, 1296, 1344, 2237
Bme18I GGWCC 6 cut(s) 48, 613, 938, 1868, 2092, 2176
BmgT120I GGNCC 6 cut(s) 48, 613, 938, 1868, 2092, 2176
BmrFI CCNGG 5 cut(s) 474, 1279, 1296, 1344, 2237
BmrI ACTGGG 2 cut(s) 944, 2119
BmsI GCATC 4 cut(s) 532, 539, 682, 826
BmtI GCTAGC 1 cut(s) 1617
BmuI ACTGGG 2 cut(s) 944, 2119
BpiI GAAGAC 1 cut(s) 249
BpmI CTGGAG 2 cut(s) 1062, 1326
Bpu1102I GCTNAGC 1 cut(s) 197
Bpu14I TTCGAA 1 cut(s) 1148
BpuEI CTTGAG 3 cut(s) 911, 1240, 2473
Bsa29I ATCGAT 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 1704
BsaJI CCNNGG 9 cut(s) 235, 730, 767, 987, 1278, 1645, 1716, 2077, 2576
BsaWI WCCGGW 2 cut(s) 45, 1870
BsaXI ACNNNNNCTCC 6 cut(s) 1470, 1500, 1521, 1551, 1857, 1887
Bsc4I CCNNNNNNNGG 5 cut(s) 832, 1375, 1851, 2185, 2413
Bse1I ACTGG 6 cut(s) 29, 939, 1606, 1813, 2125, 2327
Bse3DI GCAATG 3 cut(s) 110, 139, 1636
BseBI CCWGG 5 cut(s) 474, 1279, 1296, 1344, 2237
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 9 cut(s) 235, 730, 767, 987, 1278, 1645, 1716, 2077, 2576
BseGI GGATG 8 cut(s) 697, 841, 1249, 1323, 1411, 1495, 1782, 1944
BseLI CCNNNNNNNGG 5 cut(s) 832, 1375, 1851, 2185, 2413
BseMI GCAATG 3 cut(s) 110, 139, 1636
BseMII CTCAG 4 cut(s) 211, 495, 599, 1509
BseNI ACTGG 6 cut(s) 29, 939, 1606, 1813, 2125, 2327
BseRI GAGGAG 1 cut(s) 908
BseSI GKGCMC 1 cut(s) 686
BseXI GCAGC 3 cut(s) 1140, 1933, 2603
BshFI GGCC 7 cut(s) 596, 970, 1031, 1533, 2210, 2240, 2483
BshNI GGYRCC 3 cut(s) 4, 1274, 1940
BshVI ATCGAT 1 cut(s) 444
BsiHKAI GWGCWC 3 cut(s) 396, 607, 686
BsiSI CCGG 3 cut(s) 46, 1845, 1871
BslFI GGGAC 6 cut(s) 202, 874, 1994, 2105, 2189, 2548
BslI CCNNNNNNNGG 5 cut(s) 832, 1375, 1851, 2185, 2413
BsmAI GTCTC 4 cut(s) 730, 1342, 1704, 2502
BsmFI GGGAC 6 cut(s) 202, 874, 1994, 2105, 2189, 2548
BsnI GGCC 7 cut(s) 596, 970, 1031, 1533, 2210, 2240, 2483
Bso31I GGTCTC 1 cut(s) 1704
Bsp119I TTCGAA 1 cut(s) 1148
Bsp1286I GDGCHC 3 cut(s) 396, 607, 686
Bsp1720I GCTNAGC 1 cut(s) 197
Bsp19I CCATGG 2 cut(s) 730, 1716
BspACI CCGC 2 cut(s) 651, 2193
BspANI GGCC 7 cut(s) 596, 970, 1031, 1533, 2210, 2240, 2483
BspCNI CTCAG 4 cut(s) 210, 496, 600, 1510
BspDI ATCGAT 1 cut(s) 444
BspHI TCATGA 1 cut(s) 492
BspOI GCTAGC 1 cut(s) 1617
BspPI GGATC 8 cut(s) 934, 953, 1694, 1707, 1744, 1855, 2267, 2284
BspQI GCTCTTC 1 cut(s) 1974
BspT104I TTCGAA 1 cut(s) 1148
BspT107I GGYRCC 3 cut(s) 4, 1274, 1940
BspTNI GGTCTC 1 cut(s) 1704
BsrDI GCAATG 3 cut(s) 110, 139, 1636
BsrI ACTGG 6 cut(s) 29, 939, 1606, 1813, 2125, 2327
BssECI CCNNGG 9 cut(s) 235, 730, 767, 987, 1278, 1645, 1716, 2077, 2576
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 8 cut(s) 235, 730, 767, 987, 1645, 1716, 2077, 2576
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 5 cut(s) 474, 1279, 1296, 1344, 2237
Bst4CI ACNGT 4 cut(s) 602, 709, 2393, 2562
Bst6I CTCTTC 3 cut(s) 1666, 1974, 2255
BstBI TTCGAA 1 cut(s) 1148
BstC8I GCNNGC 5 cut(s) 1263, 1545, 1615, 1619, 1958
BstDEI CTNAG 5 cut(s) 197, 308, 504, 608, 1518
BstDSI CCRYGG 2 cut(s) 730, 1716
BstF5I GGATG 8 cut(s) 697, 841, 1249, 1323, 1411, 1495, 1782, 1944
BstHHI GCGC 1 cut(s) 2365
BstMAI GTCTC 4 cut(s) 730, 1342, 1704, 2502
BstMWI GCNNNNNNNGC 4 cut(s) 602, 886, 1167, 1176
BstNI CCWGG 5 cut(s) 474, 1279, 1296, 1344, 2237
BstNSI RCATGY 6 cut(s) 1215, 1265, 1621, 1774, 2159, 2386
BstSCI CCNGG 5 cut(s) 472, 1277, 1294, 1342, 2235
BstSFI CTRYAG 3 cut(s) 598, 1162, 2526
BstSLI GKGCMC 1 cut(s) 686
BstV1I GCAGC 3 cut(s) 1140, 1933, 2603
BstV2I GAAGAC 1 cut(s) 249
BstX2I RGATCY 4 cut(s) 458, 926, 1699, 1847
BstXI CCANNNNNNTGG 2 cut(s) 2084, 2284
BstYI RGATCY 4 cut(s) 458, 926, 1699, 1847
Bsu15I ATCGAT 1 cut(s) 444
BsuRI GGCC 7 cut(s) 596, 970, 1031, 1533, 2210, 2240, 2483
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 2 cut(s) 730, 1716
BtgZI GCGATG 1 cut(s) 532
BtsCI GGATG 8 cut(s) 697, 841, 1249, 1323, 1411, 1495, 1782, 1944
BtsIMutI CAGTG 6 cut(s) 214, 607, 1047, 2132, 2157, 2398
Cac8I GCNNGC 5 cut(s) 1263, 1545, 1615, 1619, 1958
CciI TCATGA 1 cut(s) 492
CfoI GCGC 1 cut(s) 2365
Cfr13I GGNCC 6 cut(s) 48, 613, 938, 1868, 2092, 2176
ClaI ATCGAT 1 cut(s) 444
CpoI CGGWCCG 1 cut(s) 48
CsiI ACCWGGT 2 cut(s) 472, 1294
Csp6I GTAC 5 cut(s) 477, 761, 1299, 1941, 2330
CspCI CAANNNNNGTGG 2 cut(s) 2032, 2067
CspI CGGWCCG 1 cut(s) 48
CviQI GTAC 5 cut(s) 477, 761, 1299, 1941, 2330
DdeI CTNAG 5 cut(s) 197, 308, 504, 608, 1518
Eam1104I CTCTTC 3 cut(s) 1666, 1974, 2255
EarI CTCTTC 3 cut(s) 1666, 1974, 2255
Eco130I CCWWGG 8 cut(s) 235, 730, 767, 987, 1645, 1716, 2077, 2576
Eco147I AGGCCT 1 cut(s) 596
Eco31I GGTCTC 1 cut(s) 1704
Eco32I GATATC 1 cut(s) 984
Eco47I GGWCC 6 cut(s) 48, 613, 938, 1868, 2092, 2176
Eco57I CTGAAG 2 cut(s) 141, 1482
EcoO109I RGGNCCY 2 cut(s) 938, 2092
EcoRI GAATTC 2 cut(s) 743, 1523
EcoRII CCWGG 5 cut(s) 472, 1277, 1294, 1342, 2235
EcoRV GATATC 1 cut(s) 984
EcoT14I CCWWGG 8 cut(s) 235, 730, 767, 987, 1645, 1716, 2077, 2576
ErhI CCWWGG 8 cut(s) 235, 730, 767, 987, 1645, 1716, 2077, 2576
FalI AAGNNNNNCTT 4 cut(s) 1159, 1191, 1985, 2017
FaqI GGGAC 6 cut(s) 202, 874, 1994, 2105, 2189, 2548
FbaI TGATCA 1 cut(s) 1417
FblI GTMKAC 1 cut(s) 338
Fnu4HI GCNGC 4 cut(s) 1129, 1922, 2193, 2592
FokI GGATG 8 cut(s) 704, 848, 1256, 1310, 1398, 1502, 1789, 1931
Fsp4HI GCNGC 4 cut(s) 1129, 1922, 2193, 2592
GlaI GCGC 1 cut(s) 2364
GluI GCNGC 4 cut(s) 1129, 1922, 2193, 2592
GsuI CTGGAG 2 cut(s) 1062, 1326
HaeIII GGCC 7 cut(s) 596, 970, 1031, 1533, 2210, 2240, 2483
HapII CCGG 3 cut(s) 46, 1845, 1871
HhaI GCGC 1 cut(s) 2365
Hin6I GCGC 1 cut(s) 2363
HinP1I GCGC 1 cut(s) 2363
HincII GTYRAC 1 cut(s) 2041
HindII GTYRAC 1 cut(s) 2041
HinfI GANTC 9 cut(s) 251, 844, 862, 1044, 1479, 1886, 2109, 2435, 2569
HpaII CCGG 3 cut(s) 46, 1845, 1871
HphI GGTGA 5 cut(s) 130, 1390, 1439, 1691, 2299
Hpy166II GTNNAC 7 cut(s) 339, 684, 761, 1744, 1930, 2041, 2558
Hpy8I GTNNAC 7 cut(s) 339, 684, 761, 1744, 1930, 2041, 2558
HpyAV CCTTC 4 cut(s) 706, 960, 1118, 1148
HpyCH4III ACNGT 4 cut(s) 602, 709, 2393, 2562
HpyF10VI GCNNNNNNNGC 4 cut(s) 602, 886, 1167, 1176
HpyF3I CTNAG 5 cut(s) 197, 308, 504, 608, 1518
HspAI GCGC 1 cut(s) 2363
KflI GGGWCCC 1 cut(s) 2092
KpnI GGTACC 1 cut(s) 1944
Ksp22I TGATCA 1 cut(s) 1417
LguI GCTCTTC 1 cut(s) 1974
LmnI GCTCC 4 cut(s) 399, 639, 2063, 2348
Lsp1109I GCAGC 3 cut(s) 1140, 1933, 2603
LweI GCATC 4 cut(s) 532, 539, 682, 826
MabI ACCWGGT 2 cut(s) 472, 1294
MaeIII GTNAC 6 cut(s) 136, 899, 1045, 1883, 1898, 2530
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 4 cut(s) 458, 926, 1699, 1847
MhlI GDGCHC 3 cut(s) 396, 607, 686
MlyI GAGTC 5 cut(s) 260, 856, 1053, 1488, 1880
MmeI TCCRAC 6 cut(s) 209, 591, 1077, 1997, 2317, 2370
MroXI GAANNNNTTC 2 cut(s) 1171, 1205
MseI TTAA 6 cut(s) 24, 93, 704, 804, 1290, 2460
MslI CAYNNNNRTG 4 cut(s) 584, 1775, 1827, 2282
MspA1I CMGCKG 1 cut(s) 2594
MspI CCGG 3 cut(s) 46, 1845, 1871
MspR9I CCNGG 5 cut(s) 474, 1279, 1296, 1344, 2237
MunI CAATTG 1 cut(s) 993
MvaI CCWGG 5 cut(s) 474, 1279, 1296, 1344, 2237
MwoI GCNNNNNNNGC 4 cut(s) 602, 886, 1167, 1176
NcoI CCATGG 2 cut(s) 730, 1716
NheI GCTAGC 1 cut(s) 1613
NmeAIII GCCGAG 1 cut(s) 2319
NmuCI GTSAC 6 cut(s) 136, 899, 1045, 1883, 1898, 2530
NspI RCATGY 6 cut(s) 1215, 1265, 1621, 1774, 2159, 2386
NspV TTCGAA 1 cut(s) 1148
PaeI GCATGC 2 cut(s) 1265, 1621
PagI TCATGA 1 cut(s) 492
PceI AGGCCT 1 cut(s) 596
PciI ACATGT 1 cut(s) 2382
PciSI GCTCTTC 1 cut(s) 1974
PdmI GAANNNNTTC 2 cut(s) 1171, 1205
PfeI GAWTC 4 cut(s) 844, 2109, 2435, 2569
PflMI CCANNNNNTGG 2 cut(s) 2185, 2413
PfoI TCCNGGA 1 cut(s) 1342
PkrI GCNGC 4 cut(s) 1130, 1923, 2194, 2593
PleI GAGTC 5 cut(s) 259, 856, 1052, 1487, 1880
PpsI GAGTC 5 cut(s) 259, 856, 1052, 1487, 1880
PpuMI RGGWCCY 2 cut(s) 938, 2092
PscI ACATGT 1 cut(s) 2382
PshBI ATTAAT 1 cut(s) 804
Psp5II RGGWCCY 2 cut(s) 938, 2092
Psp6I CCWGG 5 cut(s) 472, 1277, 1294, 1342, 2235
PspGI CCWGG 5 cut(s) 472, 1277, 1294, 1342, 2235
PspPI GGNCC 6 cut(s) 48, 613, 938, 1868, 2092, 2176
PspPPI RGGWCCY 2 cut(s) 938, 2092
PsrI GAACNNNNNNTAC 2 cut(s) 1156, 1188
PsuI RGATCY 4 cut(s) 458, 926, 1699, 1847
PvuII CAGCTG 1 cut(s) 2594
RsaI GTAC 5 cut(s) 478, 762, 1300, 1942, 2331
RsaNI GTAC 5 cut(s) 477, 761, 1299, 1941, 2330
RseI CAYNNNNRTG 4 cut(s) 584, 1775, 1827, 2282
Rsr2I CGGWCCG 1 cut(s) 48
RsrII CGGWCCG 1 cut(s) 48
SapI GCTCTTC 1 cut(s) 1974
SaqAI TTAA 6 cut(s) 24, 93, 704, 804, 1290, 2460
SatI GCNGC 4 cut(s) 1129, 1922, 2193, 2592
Sau96I GGNCC 6 cut(s) 48, 613, 938, 1868, 2092, 2176
SchI GAGTC 5 cut(s) 260, 856, 1053, 1488, 1880
ScrFI CCNGG 5 cut(s) 474, 1279, 1296, 1344, 2237
SduI GDGCHC 3 cut(s) 396, 607, 686
SexAI ACCWGGT 2 cut(s) 472, 1294
SfaNI GCATC 4 cut(s) 532, 539, 682, 826
SfcI CTRYAG 3 cut(s) 598, 1162, 2526
SfuI TTCGAA 1 cut(s) 1148
SinI GGWCC 6 cut(s) 48, 613, 938, 1868, 2092, 2176
SmiMI CAYNNNNRTG 4 cut(s) 584, 1775, 1827, 2282
SmlI CTYRAG 4 cut(s) 890, 1219, 2488, 2615
SmoI CTYRAG 4 cut(s) 890, 1219, 2488, 2615
SpeI ACTAGT 2 cut(s) 1136, 1384
SphI GCATGC 2 cut(s) 1265, 1621
SseBI AGGCCT 1 cut(s) 596
SsiI CCGC 2 cut(s) 651, 2193
SspI AATATT 1 cut(s) 16
StuI AGGCCT 1 cut(s) 596
StyD4I CCNGG 5 cut(s) 472, 1277, 1294, 1342, 2235
StyI CCWWGG 8 cut(s) 235, 730, 767, 987, 1645, 1716, 2077, 2576
TaaI ACNGT 4 cut(s) 602, 709, 2393, 2562
TaqI TCGA 6 cut(s) 291, 444, 533, 810, 1148, 2307
TatI WGTACW 1 cut(s) 2329
TauI GCSGC 1 cut(s) 2195
TfiI GAWTC 4 cut(s) 844, 2109, 2435, 2569
Tru1I TTAA 6 cut(s) 24, 93, 704, 804, 1290, 2460
Tru9I TTAA 6 cut(s) 24, 93, 704, 804, 1290, 2460
TscAI CASTG 6 cut(s) 214, 607, 1054, 2132, 2157, 2398
TseFI GTSAC 6 cut(s) 136, 899, 1045, 1883, 1898, 2530
TseI GCWGC 3 cut(s) 1128, 1921, 2591
Tsp45I GTSAC 6 cut(s) 136, 899, 1045, 1883, 1898, 2530
TspRI CASTG 6 cut(s) 214, 607, 1054, 2132, 2157, 2398
Van91I CCANNNNNTGG 2 cut(s) 2185, 2413
VneI GTGCAC 1 cut(s) 682
VpaK11BI GGWCC 6 cut(s) 48, 613, 938, 1868, 2092, 2176
VspI ATTAAT 1 cut(s) 804
XapI RAATTY 9 cut(s) 274, 287, 671, 743, 1237, 1523, 1835, 2028, 2598
XceI RCATGY 6 cut(s) 1215, 1265, 1621, 1774, 2159, 2386
XmiI GTMKAC 1 cut(s) 338
XmnI GAANNNNTTC 2 cut(s) 1171, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.