Rw3G024860

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002202
Physical Location & Seq
Reverse (-)
32454419 .. 32457350
2932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G024860.1

Sequence Viewer

Length: 2598 bp
ATGGCACCTCCTAATGATCCTATTAGTCCAGTTTTCAATGCGTTCCGGTCCGAGAGTTCTGGAAAATACTTTGACCTCTCTAATGGAAAGCTTAGTGTCAAAGGGATTCCATTGCTCTCTGAAGTTCCAAGCAATGTAACTTTCAGTCACTTCCACTCAGCCTGCCAATCTTCTGATGTTCCACTTCCTCTGCTCCAACGGGTTCGTGCCTCGTCCCACAAAGGTGCTTTCCTTGGATTCAACAAGGAGGTTCCCTCAGATAGGCTGCTCAACTCCTTGGGGAAACTCAGTAACAGGGATTTCCTTAGCATCTTTAGGTTCAAGACATGGTGGTCTACCATGTGGGTGGGAAACTCTGGTTCAAACTTGCAAATGGAAACCCAATGGGTGCTCTTAGATGTCCCGGAGATCAAGTCATATGTTATAATCATACCCATCATTGAAGCCAGTTTCAGGTCTGCTCTTCATCCTGGCAGTGATGACCATGTCATGATCTGTGCCGAGAGTGGTTCTAGTCAAGTGAAAGCTTCGAATTTCAATGCCATTGCTTATGTTCATGCTTCTGAAAATCCCTACAACTTGATGAAAGAAGCCTATAGTGCTGTTAGAGTCCATCTCAATAGTTTCAGGCTCTTGGAAGAGAAGACGGTCCCAAATCTAGTTAACAAATTTGGTTGGTGCACTTGGGATGCATTCTACTTAACTGTAGAACCTGTTGGTGTCTGGCATGGTGTGAAAGAATTTGTTGAAGGCGGTGTGTCCCCGCGGTTTCTCATAATTGATGATGGTTGGCAAAGCATCAATATGGATGATGAGGACCTGAAGGAGGACACAAAAAATCTAGTTCTTGGTGGGACTCAAATGACTGCTAGGCTTCACAGTTTCGAGGAGTGCAAAAAGTTCAGGAACTACAGAGGTGGCTCTATGTTGGGTCCTGATGCTCCTTCATTTGATGCCAAAAAGCCAAAACTGTTGATTGCCAAGGCAATTGAAATAGAGCATGCTGAGAAGGAACACGACAAGGCCCTTCATTCTGGAGTCACCGAGTTGTCTGTGTTCGAAACAAAACTTCAGAAGCTGAAGACAGAGTTGAATGATATCATTGGTGAAGGAGAGAGCAGTGCTTCTAATGAATGCCGTGGAAGCAGTTCTGGCAGTGACAAAAACTATGGAATGAAGGCCTTCACAGGTGACTTGAGAACCAAGTTTAAAGGATTGGATGACATATATGTATGGCATGCTCTTTGTGGTGCGTGGGGTGGTGTTAGGCCAGGTTCAACTCATCTCAGTTCAAAGATCATTCCTTGCAAAGTCTCACCTGGACTAGATGGGACGATGAATGATCTGGCTGTGGTGAAAATCATTGAGGGCGGGATTGGGCTTGTTCATCCTGATCAAGCTGACAGTTTCTATGACTCCATGCACTCTTACCTTTCTGAAGTTGGTATCACTGGAGTCAAAGTGGATGTAATTCATACACTTGAATATGTATCCGAGGAATACGGAGGCCGTGTGGAGCTTGCAAAGGCTTATTACAAGGGGTTGACTCATTCTCTTCAGAAGAACTTCAATGGGACTGGACTTATTGCTAGCATGCAGCAATGCAATGACTTCTTCTTTCTTGGCACAAAGCAAATCTCTATTGGAAGAGTCGGTGACGATTTTTGGTTTCAGGATCCGAATGGAGATCCAATGGGAGTCTACTGGTTACAAGGAGTTCATATGATCCACTGCGCTTACAACAGCATGTGGATGGGCCAGATGATACAGCCTGATTGGGACATGTTTCAATCAGACCATTTATGTGCCAAATTTCATGCAGGATCAAGAGCAATCTGTGGGGGTCCGGTTTATGTAAGTGACTCAGTTTCAGGGCATGATTTCGATCTCATTAAGAAGCTTGTTTACCCGGATGGTACCATTCCCAAATGCCAGCATTTCGCTCTCCCAACCCGGGACTGCCTCTTCAAAAACCCTTTATTTGATGATAAGACTGTCCTGAAAATTTGGAACTTTAACAAGTATGGCGGTGTCATTGGAGCTTTCAATTGTCAAGGGGCTGGTTGGGACCCCAAGGAACAGAGGATCAAAGGCCACTCAGAGTGCTACAAGCCAATCTCTTGTTCATTACATGTTCGTGAGATTGAATGGGACCAAAAGTCTGAAGCAGCCCATTTGGGCGAGGCTGAAGAGTACGTTGTCTACCTCAGCGAGGTTGATGAGCTCCGCCTGATGACCCCAAAGTCCGATGCAATTAAAATCGTCATCCAGCCATCTTCGTTTGAGATTTTGAGCTTTGTGCCGGTTAGAAAGCTAGGCCACAGCATTAAGTTCGCGCCAATTGGACTTACAAACATGTTCAACTGTGGAGGCACACTACAAGAGTTGGAGTACAAAAGTACCGCAGTCGAGTTTAGTGCCATGATCAAAGTTAAAGGTGGAGGCAAGTTCCTGGCCTACTCAAGTGAGTCACCAAAGAAATGTTGTTTGAATGGTGCTGAGGTAGCTTTTGAGTGGTCTGCTGATGGCAAATTGAACTCGAGTGTTCCTTGGGTTGAACAAGCTGCTGGCATTTCTGATGTGCTGTTTGCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

865

Amino Acids

95.94

Weight (kDa)

5.93

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 845 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AasI GACNNNNNNGTC 2 cut(s) 331, 2242
Acc65I GGTACC 1 cut(s) 1916
AccB1I GGYRCC 2 cut(s) 4, 1916
AccI GTMKAC 3 cut(s) 335, 1701, 2202
AccII CGCG 2 cut(s) 766, 2336
AciI CCGC 7 cut(s) 753, 764, 766, 1371, 2028, 2227, 2403
AclWI GGATC 7 cut(s) 11, 1670, 1682, 1683, 1720, 1831, 2093
AcsI RAATTY 5 cut(s) 532, 668, 740, 1811, 2004
AcuI CTGAAG 8 cut(s) 141, 842, 1055, 1100, 1458, 1541, 2184, 2208
AfaI GTAC 4 cut(s) 1918, 2195, 2393, 2401
AfiI CCNNNNNNNGG 5 cut(s) 261, 453, 826, 1954, 2212
AflIII ACRYGT 3 cut(s) 1782, 2131, 2355
AhdI GACNNNNNGTC 1 cut(s) 2158
AjnI CCWGG 4 cut(s) 469, 1270, 1318, 2451
AjuI GAANNNNNNNTTGG 2 cut(s) 2107, 2139
AleI CACNNNNGTG 1 cut(s) 222
Alw21I GWGCWC 3 cut(s) 393, 683, 2226
Alw26I GTCTC 1 cut(s) 1318
Alw44I GTGCAC 1 cut(s) 679
AlwI GGATC 7 cut(s) 11, 1670, 1682, 1683, 1720, 1831, 2093
AlwNI CAGNNNCTG 1 cut(s) 1078
Ama87I CYCGRG 2 cut(s) 1953, 2539
AoxI GGCC 8 cut(s) 1023, 1179, 1268, 1507, 1756, 2092, 2317, 2454
ApaLI GTGCAC 1 cut(s) 679
ApeKI GCWGC 4 cut(s) 265, 1597, 2168, 2564
ApoI RAATTY 5 cut(s) 532, 668, 740, 1811, 2004
Asp700I GAANNNNTTC 3 cut(s) 1147, 1181, 1565
Asp718I GGTACC 1 cut(s) 1916
AspLEI GCGC 2 cut(s) 1736, 2338
AspS9I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
AsuC2I CCSGG 4 cut(s) 404, 1910, 1954, 1955
AsuHPI GGTGA 7 cut(s) 1033, 1118, 1202, 1308, 1366, 1667, 2463
AsuII TTCGAA 2 cut(s) 530, 1059
AsuNHI GCTAGC 1 cut(s) 1589
AvaI CYCGRG 2 cut(s) 1953, 2539
AvaII GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
BaeGI GKGCMC 1 cut(s) 683
BaeI ACNNNNGTAYC 2 cut(s) 2383, 2416
BamHI GGATCC 1 cut(s) 1675
BanI GGYRCC 2 cut(s) 4, 1916
BanII GRGCYC 1 cut(s) 2226
BarI GAAGNNNNNNTAC 2 cut(s) 1889, 1921
BbsI GAAGAC 2 cut(s) 650, 1088
Bbv12I GWGCWC 3 cut(s) 393, 683, 2226
BbvCI CCTCAGC 2 cut(s) 2207, 2499
BbvI GCAGC 4 cut(s) 252, 1609, 2180, 2551
BccI CCATC 8 cut(s) 443, 621, 779, 1322, 1747, 1907, 2281, 2519
BceAI ACGGC 2 cut(s) 1122, 1494
BcgI CGANNNNNNTGC 4 cut(s) 2314, 2348, 2399, 2433
BciT130I CCWGG 4 cut(s) 471, 1272, 1320, 2453
BciVI GTATCC 1 cut(s) 1501
BclI TGATCA 2 cut(s) 1393, 2424
BcnI CCSGG 4 cut(s) 404, 1910, 1954, 1955
BcoDI GTCTC 1 cut(s) 1318
BfaI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
BfmI CTRYAG 3 cut(s) 595, 705, 910
BfuI GTATCC 1 cut(s) 1501
BisI GCNGC 4 cut(s) 266, 1598, 2169, 2565
BlsI GCNGC 4 cut(s) 267, 1599, 2170, 2566
Bme1390I CCNGG 8 cut(s) 404, 471, 1272, 1320, 1910, 1954, 1955, 2453
Bme18I GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
BmeRI GACNNNNNGTC 1 cut(s) 2158
BmeT110I CYCGRG 2 cut(s) 1953, 2539
BmgT120I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
BmrFI CCNGG 8 cut(s) 404, 471, 1272, 1320, 1910, 1954, 1955, 2453
BmsI GCATC 6 cut(s) 318, 679, 807, 928, 943, 2239
BmtI GCTAGC 1 cut(s) 1593
BpiI GAAGAC 2 cut(s) 650, 1088
BplI GAGNNNNNCTC 4 cut(s) 239, 271, 600, 632
BpmI CTGGAG 2 cut(s) 1056, 1473
Bpu10I CCTNAGC 3 cut(s) 305, 2207, 2499
Bpu14I TTCGAA 2 cut(s) 530, 1059
BpuEI CTTGAG 2 cut(s) 1216, 2446
BpuMI CCSGG 4 cut(s) 404, 1910, 1954, 1955
BsaBI GATNNNNATC 1 cut(s) 1884
BsaJI CCNNGG 9 cut(s) 232, 276, 764, 981, 1138, 1494, 1953, 2073, 2549
BsaWI WCCGGW 2 cut(s) 45, 1846
BsaXI ACNNNNNCTCC 6 cut(s) 398, 428, 1446, 1476, 1497, 1527
Bsc4I CCNNNNNNNGG 5 cut(s) 261, 453, 826, 1954, 2212
Bse118I RCCGGY 1 cut(s) 2302
Bse1I ACTGG 5 cut(s) 29, 447, 1456, 1582, 1709
Bse3DI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
Bse8I GATNNNNATC 1 cut(s) 1884
BseBI CCWGG 4 cut(s) 471, 1272, 1320, 2453
BseDI CCNNGG 9 cut(s) 232, 276, 764, 981, 1138, 1494, 1953, 2073, 2549
BseGI GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BseJI GATNNNNATC 1 cut(s) 1884
BseLI CCNNNNNNNGG 5 cut(s) 261, 453, 826, 1954, 2212
BseMI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
BseMII CTCAG 9 cut(s) 171, 270, 301, 996, 1300, 1878, 2112, 2221, 2490
BseNI ACTGG 5 cut(s) 29, 447, 1456, 1582, 1709
BseRI GAGGAG 1 cut(s) 902
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 4 cut(s) 252, 1609, 2180, 2551
Bsh1236I CGCG 2 cut(s) 766, 2336
BshFI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BshNI GGYRCC 2 cut(s) 4, 1916
BsiHKAI GWGCWC 3 cut(s) 393, 683, 2226
BsiHKCI CYCGRG 2 cut(s) 1953, 2539
BsiSI CCGG 6 cut(s) 46, 404, 1847, 1910, 1954, 2303
BslI CCNNNNNNNGG 5 cut(s) 261, 453, 826, 1954, 2212
BsmAI GTCTC 1 cut(s) 1318
BsmI GAATGC 2 cut(s) 692, 1139
BsnI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BsoBI CYCGRG 2 cut(s) 1953, 2539
Bsp119I TTCGAA 2 cut(s) 530, 1059
Bsp1286I GDGCHC 3 cut(s) 393, 683, 2226
BspACI CCGC 7 cut(s) 753, 764, 766, 1371, 2028, 2227, 2403
BspANI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BspCNI CTCAG 9 cut(s) 170, 269, 300, 997, 1299, 1877, 2111, 2220, 2491
BspFNI CGCG 2 cut(s) 766, 2336
BspHI TCATGA 1 cut(s) 489
BspOI GCTAGC 1 cut(s) 1593
BspPI GGATC 7 cut(s) 11, 1670, 1682, 1683, 1720, 1831, 2093
BspQI GCTCTTC 1 cut(s) 468
BspT104I TTCGAA 2 cut(s) 530, 1059
BspT107I GGYRCC 2 cut(s) 4, 1916
BsrDI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
BsrFI RCCGGY 1 cut(s) 2302
BsrI ACTGG 5 cut(s) 29, 447, 1456, 1582, 1709
BssAI RCCGGY 1 cut(s) 2302
BssECI CCNNGG 9 cut(s) 232, 276, 764, 981, 1138, 1494, 1953, 2073, 2549
BssT1I CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
Bst2UI CCWGG 4 cut(s) 471, 1272, 1320, 2453
Bst4CI ACNGT 7 cut(s) 649, 706, 881, 972, 1406, 1996, 2366
Bst6I CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
BstBI TTCGAA 2 cut(s) 530, 1059
BstC8I GCNNGC 8 cut(s) 163, 1002, 1239, 1521, 1591, 1595, 1934, 2569
BstDSI CCRYGG 2 cut(s) 764, 1138
BstENI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
BstF5I GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BstFNI CGCG 2 cut(s) 766, 2336
BstHHI GCGC 2 cut(s) 1736, 2338
BstMAI GTCTC 1 cut(s) 1318
BstMWI GCNNNNNNNGC 4 cut(s) 599, 1143, 1152, 2504
BstNI CCWGG 4 cut(s) 471, 1272, 1320, 2453
BstNSI RCATGY 7 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359
BstSCI CCNGG 8 cut(s) 402, 469, 1270, 1318, 1908, 1952, 1953, 2451
BstSFI CTRYAG 3 cut(s) 595, 705, 910
BstSLI GKGCMC 1 cut(s) 683
BstUI CGCG 2 cut(s) 766, 2336
BstV1I GCAGC 4 cut(s) 252, 1609, 2180, 2551
BstV2I GAAGAC 2 cut(s) 650, 1088
BstX2I RGATCY 2 cut(s) 1675, 1687
BstXI CCANNNNNNTGG 1 cut(s) 346
BstYI RGATCY 2 cut(s) 1675, 1687
BsuI GTATCC 1 cut(s) 1501
BsuRI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BtgI CCRYGG 2 cut(s) 764, 1138
BtsCI GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BtsI GCAGTG 4 cut(s) 481, 1126, 1162, 1729
BtsIMutI CAGTG 5 cut(s) 481, 1126, 1162, 1449, 1729
Cac8I GCNNGC 8 cut(s) 163, 1002, 1239, 1521, 1591, 1595, 1934, 2569
CaiI CAGNNNCTG 1 cut(s) 1078
CciI TCATGA 1 cut(s) 489
CfoI GCGC 2 cut(s) 1736, 2338
Cfr10I RCCGGY 1 cut(s) 2302
Cfr13I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
Cfr42I CCGCGG 1 cut(s) 767
Cfr9I CCCGGG 1 cut(s) 1953
CpoI CGGWCCG 1 cut(s) 48
Csp6I GTAC 4 cut(s) 1917, 2194, 2392, 2400
CspI CGGWCCG 1 cut(s) 48
CviQI GTAC 4 cut(s) 1917, 2194, 2392, 2400
DraI TTTAAA 1 cut(s) 1210
DrdI GACNNNNNNGTC 2 cut(s) 331, 2242
DriI GACNNNNNGTC 1 cut(s) 2158
DseDI GACNNNNNNGTC 2 cut(s) 331, 2242
Eam1104I CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
Eam1105I GACNNNNNGTC 1 cut(s) 2158
EarI CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
EciI GGCGGA 1 cut(s) 2216
Ecl136II GAGCTC 1 cut(s) 2224
Eco130I CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
Eco147I AGGCCT 1 cut(s) 1181
Eco24I GRGCYC 1 cut(s) 2226
Eco32I GATATC 1 cut(s) 1099
Eco47I GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
Eco53kI GAGCTC 1 cut(s) 2224
Eco57I CTGAAG 8 cut(s) 141, 842, 1055, 1100, 1458, 1541, 2184, 2208
Eco88I CYCGRG 2 cut(s) 1953, 2539
EcoICRI GAGCTC 1 cut(s) 2224
EcoNI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
EcoO109I RGGNCCY 4 cut(s) 817, 932, 1024, 2068
EcoRII CCWGG 4 cut(s) 469, 1270, 1318, 2451
EcoRV GATATC 1 cut(s) 1099
EcoT14I CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 2226
ErhI CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
FauI CCCGC 2 cut(s) 771, 1364
FauNDI CATATG 2 cut(s) 418, 1722
FbaI TGATCA 2 cut(s) 1393, 2424
FblI GTMKAC 3 cut(s) 335, 1701, 2202
Fnu4HI GCNGC 4 cut(s) 266, 1598, 2169, 2565
FokI GGATG 9 cut(s) 453, 701, 821, 1232, 1374, 1478, 1765, 1925, 2252
FriOI GRGCYC 1 cut(s) 2226
Fsp4HI GCNGC 4 cut(s) 266, 1598, 2169, 2565
FspBI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
GlaI GCGC 2 cut(s) 1735, 2337
GluI GCNGC 4 cut(s) 266, 1598, 2169, 2565
GsuI CTGGAG 2 cut(s) 1056, 1473
HaeIII GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
HapII CCGG 6 cut(s) 46, 404, 1847, 1910, 1954, 2303
HhaI GCGC 2 cut(s) 1736, 2338
Hin6I GCGC 2 cut(s) 1734, 2336
HinP1I GCGC 2 cut(s) 1734, 2336
HincII GTYRAC 2 cut(s) 664, 1545
HindII GTYRAC 2 cut(s) 664, 1545
HindIII AAGCTT 3 cut(s) 89, 525, 1898
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 6 cut(s) 46, 404, 1847, 1910, 1954, 2303
HphI GGTGA 7 cut(s) 1033, 1118, 1202, 1308, 1366, 1667, 2463
Hpy166II GTNNAC 7 cut(s) 336, 664, 681, 1545, 1702, 1906, 2203
Hpy8I GTNNAC 7 cut(s) 336, 664, 681, 1545, 1702, 1906, 2203
HpyAV CCTTC 8 cut(s) 743, 817, 954, 1003, 1037, 1103, 1171, 1192
HpyCH4III ACNGT 7 cut(s) 649, 706, 881, 972, 1406, 1996, 2366
HpyCH4IV ACGT 1 cut(s) 2196
HpyF10VI GCNNNNNNNGC 4 cut(s) 599, 1143, 1152, 2504
HpySE526I ACGT 1 cut(s) 2196
HspAI GCGC 2 cut(s) 1734, 2336
KflI GGGWCCC 1 cut(s) 2068
KpnI GGTACC 1 cut(s) 1920
Ksp22I TGATCA 2 cut(s) 1393, 2424
KspAI GTTAAC 1 cut(s) 664
KspI CCGCGG 1 cut(s) 767
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 5 cut(s) 198, 946, 1516, 2039, 2229
Lsp1109I GCAGC 4 cut(s) 252, 1609, 2180, 2551
LweI GCATC 6 cut(s) 318, 679, 807, 928, 943, 2239
MaeI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
MaeII ACGT 1 cut(s) 2196
MfeI CAATTG 3 cut(s) 987, 2047, 2340
MflI RGATCY 2 cut(s) 1675, 1687
MhlI GDGCHC 3 cut(s) 393, 683, 2226
MmeI TCCRAC 2 cut(s) 220, 2367
Mph1103I ATGCAT 1 cut(s) 694
MroXI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
MseI TTAA 8 cut(s) 663, 701, 1209, 1893, 2016, 2256, 2328, 2433
MslI CAYNNNNRTG 6 cut(s) 222, 344, 803, 1751, 1803, 2136
MspA1I CMGCKG 1 cut(s) 766
MspI CCGG 6 cut(s) 46, 404, 1847, 1910, 1954, 2303
MspR9I CCNGG 8 cut(s) 404, 471, 1272, 1320, 1910, 1954, 1955, 2453
MunI CAATTG 3 cut(s) 987, 2047, 2340
Mva1269I GAATGC 2 cut(s) 692, 1139
MvaI CCWGG 4 cut(s) 471, 1272, 1320, 2453
MvnI CGCG 2 cut(s) 766, 2336
MwoI GCNNNNNNNGC 4 cut(s) 599, 1143, 1152, 2504
NciI CCSGG 4 cut(s) 404, 1910, 1954, 1955
NdeI CATATG 2 cut(s) 418, 1722
NheI GCTAGC 1 cut(s) 1589
NmeAIII GCCGAG 1 cut(s) 526
NmuCI GTSAC 7 cut(s) 146, 1039, 1157, 1190, 1655, 1859, 2469
NsiI ATGCAT 1 cut(s) 694
NspI RCATGY 7 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359
NspV TTCGAA 2 cut(s) 530, 1059
OliI CACNNNNGTG 1 cut(s) 222
PaeI GCATGC 3 cut(s) 1004, 1241, 1597
PaeR7I CTCGAG 1 cut(s) 2539
PagI TCATGA 1 cut(s) 489
PceI AGGCCT 1 cut(s) 1181
PciI ACATGT 3 cut(s) 1782, 2131, 2355
PciSI GCTCTTC 1 cut(s) 468
PctI GAATGC 2 cut(s) 692, 1139
PdmI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
PfeI GAWTC 2 cut(s) 106, 237
PflFI GACNNNGTC 1 cut(s) 485
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 4 cut(s) 267, 1599, 2170, 2566
PpuMI RGGWCCY 3 cut(s) 817, 932, 2068
PscI ACATGT 3 cut(s) 1782, 2131, 2355
PsiI TTATAA 1 cut(s) 425
Psp124BI GAGCTC 1 cut(s) 2226
Psp5II RGGWCCY 3 cut(s) 817, 932, 2068
Psp6I CCWGG 4 cut(s) 469, 1270, 1318, 2451
PspGI CCWGG 4 cut(s) 469, 1270, 1318, 2451
PspPI GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
PspPPI RGGWCCY 3 cut(s) 817, 932, 2068
PspXI VCTCGAGB 1 cut(s) 2539
PstNI CAGNNNCTG 1 cut(s) 1078
PsuI RGATCY 2 cut(s) 1675, 1687
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 4 cut(s) 1918, 2195, 2393, 2401
RsaNI GTAC 4 cut(s) 1917, 2194, 2392, 2400
RseI CAYNNNNRTG 6 cut(s) 222, 344, 803, 1751, 1803, 2136
Rsr2I CGGWCCG 1 cut(s) 48
RsrII CGGWCCG 1 cut(s) 48
SacI GAGCTC 1 cut(s) 2226
SacII CCGCGG 1 cut(s) 767
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 8 cut(s) 663, 701, 1209, 1893, 2016, 2256, 2328, 2433
SatI GCNGC 4 cut(s) 266, 1598, 2169, 2565
Sau96I GGNCC 9 cut(s) 48, 649, 817, 932, 1024, 1756, 1844, 2068, 2152
ScrFI CCNGG 8 cut(s) 404, 471, 1272, 1320, 1910, 1954, 1955, 2453
SduI GDGCHC 3 cut(s) 393, 683, 2226
SfaNI GCATC 6 cut(s) 318, 679, 807, 928, 943, 2239
SfcI CTRYAG 3 cut(s) 595, 705, 910
Sfr274I CTCGAG 1 cut(s) 2539
Sfr303I CCGCGG 1 cut(s) 767
SfuI TTCGAA 2 cut(s) 530, 1059
SgrBI CCGCGG 1 cut(s) 767
SinI GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
SlaI CTCGAG 1 cut(s) 2539
SmaI CCCGGG 1 cut(s) 1955
SmiMI CAYNNNNRTG 6 cut(s) 222, 344, 803, 1751, 1803, 2136
SmlI CTYRAG 3 cut(s) 1195, 2461, 2539
SmoI CTYRAG 3 cut(s) 1195, 2461, 2539
SphI GCATGC 3 cut(s) 1004, 1241, 1597
SseBI AGGCCT 1 cut(s) 1181
SsiI CCGC 7 cut(s) 753, 764, 766, 1371, 2028, 2227, 2403
SspMI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
SstI GAGCTC 1 cut(s) 2226
StuI AGGCCT 1 cut(s) 1181
StyD4I CCNGG 8 cut(s) 402, 469, 1270, 1318, 1908, 1952, 1953, 2451
StyI CCWWGG 5 cut(s) 232, 276, 981, 2073, 2549
TaaI ACNGT 7 cut(s) 649, 706, 881, 972, 1406, 1996, 2366
TaiI ACGT 1 cut(s) 2199
TaqI TCGA 6 cut(s) 530, 885, 1059, 1884, 2409, 2540
TatI WGTACW 1 cut(s) 2391
TfiI GAWTC 2 cut(s) 106, 237
Tru1I TTAA 8 cut(s) 663, 701, 1209, 1893, 2016, 2256, 2328, 2433
Tru9I TTAA 8 cut(s) 663, 701, 1209, 1893, 2016, 2256, 2328, 2433
TscAI CASTG 5 cut(s) 481, 1126, 1162, 1456, 1736
TseFI GTSAC 7 cut(s) 146, 1039, 1157, 1190, 1655, 1859, 2469
TseI GCWGC 4 cut(s) 265, 1597, 2168, 2564
Tsp45I GTSAC 7 cut(s) 146, 1039, 1157, 1190, 1655, 1859, 2469
TspGWI ACGGA 1 cut(s) 1518
TspMI CCCGGG 1 cut(s) 1953
TspRI CASTG 5 cut(s) 481, 1126, 1162, 1456, 1736
Tth111I GACNNNGTC 1 cut(s) 485
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 7 cut(s) 48, 649, 817, 932, 1844, 2068, 2152
XagI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
XapI RAATTY 5 cut(s) 532, 668, 740, 1811, 2004
XceI RCATGY 7 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359
XhoI CTCGAG 1 cut(s) 2539
XmaI CCCGGG 1 cut(s) 1953
XmiI GTMKAC 3 cut(s) 335, 1701, 2202
XmnI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
XspI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.