RchiOBHm_Chr3g0487311

Stachyose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002202
Physical Location & Seq
Reverse (-)
34493726 .. 34495142
1417 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45174

Sequence Viewer

Length: 1368 bp
ATGGCACCTCCTAATGATCCTCTTAGTCCAGTTTTCAAGGCGTTCCGGTCCGAGAGTTCTGGACAATACTTTGACCTCTCTAATGGAAAGCTTAGTGTCAAAGGTGTTCCATTGCTCTCTGAAGTTCCAAGCAATGTAACTTTCAGGGACTTCCACTCACCCTGCCAATCCTCTGATGCTCCACTTCCTCTGCTCCAACGGGTTCGTGCTTCATCCCACAAGGGTGCTTTCCTTGGATTCAACAAGGAGGTTCCCTCAGATAGGCTGCTCAACTCCTTGGGGAAACTCAGTAACAGGGATTTCCTTAGCATCTTTAGGTTCAAGACATGGTGGTCTACCATGTGGGTAGGAAACTCTGGTTCAAACTTGCAAATGGAAACCCAATGGGTGCTCTTAGATGTCCCGGAGATCAAGTCCTATGTTATAATCATACCGATCATTGAAGCCAATTTCAGGTCTGCTCTTCATCCTGGCAGTGATGACCATGTCATGATCTGTGCCGAGAGTGGTTCTAGTCAAGTAAAAGCGTCGAATTTCGATGCCATTGCTTATGTTAATGCTTCTGACAATCCCTACAACTTGATGAAAGAAGCCTATAGTGCTGTTAGAGTCCATCTCAATAGTTTTAGGCTCTTGGAAGAGAAGACGGTCCCAAATCTAGTTAACAAATTCGGTTGGTGCACTTGGGATGCATTCTACTTAAATGTAGAACCTGTTGGTGTCTGGCATGGTCTGAATGAATTTATTGAAGGCGGTGTGTCCCCGCGGTTTCTCATCATTGATGATGGTTGGCAAAGCATCAATATGGATGATGAGGACCTGAAGGAGGAAACGAAAAAACTAGTTCATGGTGGTACTAAAATGATTGCTAGGCTTCACAGGTTTGAGGAGTGCAAGAAGTTCAGGAACTACAGAGGTGGGTCTATGTTGGGTCTTGACGCTCCTTCACATGCTGAGAAGGATTGCGACAAGGCCCTTCATTCTGGAGTCACTGAGTTATCTGTGTTTGAAACTAAACTTCAGAAGCTGAAGATAGAGTTGAATGAGATCATTGGTGGAGGAGAGAGCAGTGCTCCTAGTGAAGGCCGTGGAACCAGTTCTTGCAGTGATGAAAATTATGGAATGAGGGCTTTCACCGGTGACTTGAGAACCAAGTTTAAAGGTTTGGATGATATATATGTATGGCATGCCATTTGTGGTGCCTGGGGTGGTGTTAGGCCAGGTTCAACTCATCTCAGTTCAAAGATCATTCCTTGCAAAGTCTCACCTGGACTAGACGGGACAATGAATGATTCCGCTGTGGTGAAAATCGTTGAGAGCGGGATTGGGCTTGTTCATCCTGATGAAGCTGATAGTTTCTATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009628 GO:0016740 GO:0016757 GO:0016758 GO:0047268 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

455

Amino Acids

50.35

Weight (kDa)

5.76

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 455 1.3e-136 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AasI GACNNNNNNGTC 1 cut(s) 331
AccB1I GGYRCC 2 cut(s) 4, 1199
AccBSI CCGCTC 1 cut(s) 1320
AccI GTMKAC 1 cut(s) 335
AccII CGCG 1 cut(s) 766
AciI CCGC 5 cut(s) 753, 764, 766, 1296, 1320
AclWI GGATC 1 cut(s) 11
AcsI RAATTY 3 cut(s) 532, 668, 740
AcuI CTGAAG 4 cut(s) 141, 842, 1004, 1049
AfaI GTAC 1 cut(s) 856
AfiI CCNNNNNNNGG 4 cut(s) 261, 453, 826, 1082
AgeI ACCGGT 1 cut(s) 1136
AhlI ACTAGT 1 cut(s) 841
AjnI CCWGG 4 cut(s) 469, 1202, 1219, 1267
AleI CACNNNNGTG 1 cut(s) 222
AluBI AGCT 3 cut(s) 91, 1027, 1349
AluI AGCT 3 cut(s) 91, 1027, 1349
Alw21I GWGCWC 3 cut(s) 393, 683, 1075
Alw26I GTCTC 1 cut(s) 1267
Alw44I GTGCAC 1 cut(s) 679
AlwI GGATC 1 cut(s) 11
AlwNI CAGNNNCTG 1 cut(s) 1027
AoxI GGCC 3 cut(s) 972, 1084, 1217
ApaLI GTGCAC 1 cut(s) 679
ApeKI GCWGC 1 cut(s) 265
ApoI RAATTY 3 cut(s) 532, 668, 740
AsiGI ACCGGT 1 cut(s) 1136
Asp700I GAANNNNTTC 1 cut(s) 1096
AspS9I GGNCC 4 cut(s) 48, 649, 817, 973
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 5 cut(s) 150, 1126, 1151, 1257, 1315
AvaII GGWCC 3 cut(s) 48, 649, 817
BaeGI GKGCMC 1 cut(s) 683
BanI GGYRCC 2 cut(s) 4, 1199
BbsI GAAGAC 1 cut(s) 650
Bbv12I GWGCWC 3 cut(s) 393, 683, 1075
BbvI GCAGC 1 cut(s) 252
BccI CCATC 2 cut(s) 621, 779
BceAI ACGGC 1 cut(s) 1071
BcgI CGANNNNNNTGC 2 cut(s) 527, 561
BciT130I CCWGG 4 cut(s) 471, 1204, 1221, 1269
BcnI CCSGG 1 cut(s) 404
BcoDI GTCTC 1 cut(s) 1267
BcuI ACTAGT 1 cut(s) 841
BfaI CTAG 6 cut(s) 513, 659, 842, 870, 1077, 1274
BfmI CTRYAG 2 cut(s) 595, 910
BisI GCNGC 1 cut(s) 266
BlsI GCNGC 1 cut(s) 267
Bme1390I CCNGG 5 cut(s) 404, 471, 1204, 1221, 1269
Bme18I GGWCC 3 cut(s) 48, 649, 817
BmgT120I GGNCC 4 cut(s) 48, 649, 817, 973
BmiI GGNNCC 5 cut(s) 6, 252, 651, 1093, 1201
BmrFI CCNGG 5 cut(s) 404, 471, 1204, 1221, 1269
BmsI GCATC 5 cut(s) 166, 318, 529, 679, 807
BpiI GAAGAC 1 cut(s) 650
BplI GAGNNNNNCTC 6 cut(s) 239, 271, 600, 632, 1057, 1089
BpmI CTGGAG 1 cut(s) 1005
Bpu10I CCTNAGC 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 1165
BpuMI CCSGG 1 cut(s) 404
BsaJI CCNNGG 5 cut(s) 232, 276, 764, 1087, 1203
BsaWI WCCGGW 2 cut(s) 45, 1136
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 4 cut(s) 261, 453, 826, 1082
Bse118I RCCGGY 1 cut(s) 1136
Bse1I ACTGG 2 cut(s) 29, 1095
Bse3DI GCAATG 3 cut(s) 110, 139, 543
BseBI CCWGG 4 cut(s) 471, 1204, 1221, 1269
BseDI CCNNGG 5 cut(s) 232, 276, 764, 1087, 1203
BseGI GGATG 6 cut(s) 212, 466, 694, 814, 1174, 1336
BseLI CCNNNNNNNGG 4 cut(s) 261, 453, 826, 1082
BseMI GCAATG 3 cut(s) 110, 139, 543
BseMII CTCAG 5 cut(s) 270, 301, 945, 984, 1249
BseNI ACTGG 2 cut(s) 29, 1095
BseRI GAGGAG 2 cut(s) 902, 1074
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 1 cut(s) 252
Bsh1236I CGCG 1 cut(s) 766
BshFI GGCC 3 cut(s) 974, 1086, 1219
BshNI GGYRCC 2 cut(s) 4, 1199
BshTI ACCGGT 1 cut(s) 1136
BsiHKAI GWGCWC 3 cut(s) 393, 683, 1075
BsiSI CCGG 3 cut(s) 46, 404, 1137
BslFI GGGAC 5 cut(s) 161, 386, 635, 745, 1294
BslI CCNNNNNNNGG 4 cut(s) 261, 453, 826, 1082
BsmAI GTCTC 1 cut(s) 1267
BsmFI GGGAC 5 cut(s) 161, 386, 635, 745, 1294
BsmI GAATGC 1 cut(s) 692
BsnI GGCC 3 cut(s) 974, 1086, 1219
Bsp1286I GDGCHC 3 cut(s) 393, 683, 1075
Bsp143I GATC 6 cut(s) 16, 408, 435, 492, 1047, 1245
BspACI CCGC 5 cut(s) 753, 764, 766, 1296, 1320
BspANI GGCC 3 cut(s) 974, 1086, 1219
BspCNI CTCAG 5 cut(s) 269, 300, 946, 985, 1248
BspFNI CGCG 1 cut(s) 766
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 5 cut(s) 6, 252, 651, 1093, 1201
BspPI GGATC 1 cut(s) 11
BspQI GCTCTTC 1 cut(s) 468
BspT107I GGYRCC 2 cut(s) 4, 1199
BsrBI CCGCTC 1 cut(s) 1320
BsrDI GCAATG 3 cut(s) 110, 139, 543
BsrFI RCCGGY 1 cut(s) 1136
BsrI ACTGG 2 cut(s) 29, 1095
BssAI RCCGGY 1 cut(s) 1136
BssECI CCNNGG 5 cut(s) 232, 276, 764, 1087, 1203
BssMI GATC 6 cut(s) 16, 408, 435, 492, 1047, 1245
BssT1I CCWWGG 2 cut(s) 232, 276
Bst2UI CCWGG 4 cut(s) 471, 1204, 1221, 1269
Bst4CI ACNGT 1 cut(s) 649
Bst6I CTCTTC 2 cut(s) 468, 633
BstC8I GCNNGC 1 cut(s) 1188
BstDEI CTNAG 9 cut(s) 23, 92, 256, 287, 305, 394, 954, 993, 1235
BstDSI CCRYGG 2 cut(s) 764, 1087
BstENI CCTNNNNNAGG 3 cut(s) 259, 824, 1080
BstF5I GGATG 6 cut(s) 212, 466, 694, 814, 1174, 1336
BstFNI CGCG 1 cut(s) 766
BstKTI GATC 6 cut(s) 19, 411, 438, 495, 1050, 1248
BstMAI GTCTC 1 cut(s) 1267
BstMBI GATC 6 cut(s) 16, 408, 435, 492, 1047, 1245
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstNI CCWGG 4 cut(s) 471, 1204, 1221, 1269
BstNSI RCATGY 2 cut(s) 953, 1190
BstSCI CCNGG 5 cut(s) 402, 469, 1202, 1219, 1267
BstSFI CTRYAG 2 cut(s) 595, 910
BstSLI GKGCMC 1 cut(s) 683
BstUI CGCG 1 cut(s) 766
BstV1I GCAGC 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 650
BsuRI GGCC 3 cut(s) 974, 1086, 1219
BtgI CCRYGG 2 cut(s) 764, 1087
BtsCI GGATG 6 cut(s) 212, 466, 694, 814, 1174, 1336
BtsI GCAGTG 3 cut(s) 481, 1075, 1111
BtsIMutI CAGTG 4 cut(s) 481, 990, 1075, 1111
Cac8I GCNNGC 1 cut(s) 1188
CaiI CAGNNNCTG 1 cut(s) 1027
CciI TCATGA 1 cut(s) 489
Cfr10I RCCGGY 1 cut(s) 1136
Cfr13I GGNCC 4 cut(s) 48, 649, 817, 973
Cfr42I CCGCGG 1 cut(s) 767
CpoI CGGWCCG 1 cut(s) 48
CseI GACGC 2 cut(s) 516, 947
Csp6I GTAC 1 cut(s) 855
CspAI ACCGGT 1 cut(s) 1136
CspI CGGWCCG 1 cut(s) 48
CviAII CATG 8 cut(s) 327, 340, 485, 490, 728, 848, 950, 1187
CviQI GTAC 1 cut(s) 855
DdeI CTNAG 9 cut(s) 23, 92, 256, 287, 305, 394, 954, 993, 1235
DpnI GATC 6 cut(s) 18, 410, 437, 494, 1049, 1247
DpnII GATC 6 cut(s) 16, 408, 435, 492, 1047, 1245
DraI TTTAAA 1 cut(s) 1159
DrdI GACNNNNNNGTC 1 cut(s) 331
DseDI GACNNNNNNGTC 1 cut(s) 331
Eam1104I CTCTTC 2 cut(s) 468, 633
EarI CTCTTC 2 cut(s) 468, 633
Eco130I CCWWGG 2 cut(s) 232, 276
Eco47I GGWCC 3 cut(s) 48, 649, 817
Eco57I CTGAAG 4 cut(s) 141, 842, 1004, 1049
EcoNI CCTNNNNNAGG 3 cut(s) 259, 824, 1080
EcoO109I RGGNCCY 2 cut(s) 817, 973
EcoRII CCWGG 4 cut(s) 469, 1202, 1219, 1267
EcoT14I CCWWGG 2 cut(s) 232, 276
EcoT22I ATGCAT 1 cut(s) 694
ErhI CCWWGG 2 cut(s) 232, 276
FaeI CATG 8 cut(s) 330, 343, 488, 493, 731, 851, 953, 1190
FaqI GGGAC 5 cut(s) 161, 386, 635, 745, 1294
FatI CATG 8 cut(s) 326, 339, 484, 489, 727, 847, 949, 1186
FauI CCCGC 2 cut(s) 771, 1313
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 1 cut(s) 266
FokI GGATG 6 cut(s) 199, 453, 701, 821, 1181, 1323
Fsp4HI GCNGC 1 cut(s) 266
FspBI CTAG 6 cut(s) 513, 659, 842, 870, 1077, 1274
GluI GCNGC 1 cut(s) 266
GsuI CTGGAG 1 cut(s) 1005
HaeIII GGCC 3 cut(s) 974, 1086, 1219
HapII CCGG 3 cut(s) 46, 404, 1137
HgaI GACGC 2 cut(s) 516, 947
Hin1II CATG 8 cut(s) 330, 343, 488, 493, 731, 851, 953, 1190
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 4 cut(s) 237, 609, 987, 1292
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 3 cut(s) 46, 404, 1137
HphI GGTGA 5 cut(s) 150, 1126, 1151, 1257, 1315
Hpy166II GTNNAC 3 cut(s) 336, 664, 681
Hpy188I TCNGA 7 cut(s) 52, 121, 175, 259, 565, 735, 1023
Hpy188III TCNNGA 7 cut(s) 60, 322, 490, 904, 935, 984, 1340
Hpy8I GTNNAC 3 cut(s) 336, 664, 681
Hpy99I CGWCG 1 cut(s) 532
HpyAV CCTTC 6 cut(s) 743, 817, 952, 954, 986, 1076
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4V TGCA 6 cut(s) 370, 681, 692, 894, 1104, 1257
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
HpyF3I CTNAG 9 cut(s) 23, 92, 256, 287, 305, 394, 954, 993, 1235
Hsp92II CATG 8 cut(s) 330, 343, 488, 493, 731, 851, 953, 1190
KspAI GTTAAC 1 cut(s) 664
KspI CCGCGG 1 cut(s) 767
Kzo9I GATC 6 cut(s) 16, 408, 435, 492, 1047, 1245
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 4 cut(s) 184, 198, 946, 1078
Lsp1109I GCAGC 1 cut(s) 252
LweI GCATC 5 cut(s) 166, 318, 529, 679, 807
MaeI CTAG 6 cut(s) 513, 659, 842, 870, 1077, 1274
MaeIII GTNAC 4 cut(s) 136, 290, 988, 1139
MalI GATC 6 cut(s) 18, 410, 437, 494, 1049, 1247
MbiI CCGCTC 1 cut(s) 1320
MboI GATC 6 cut(s) 16, 408, 435, 492, 1047, 1245
MboII GAAGA 4 cut(s) 455, 650, 655, 1042
MhlI GDGCHC 3 cut(s) 393, 683, 1075
MluCI AATT 5 cut(s) 448, 532, 668, 740, 1114
MlyI GAGTC 2 cut(s) 618, 996
MmeI TCCRAC 1 cut(s) 220
Mph1103I ATGCAT 1 cut(s) 694
MroXI GAANNNNTTC 1 cut(s) 1096
MseI TTAA 4 cut(s) 555, 663, 701, 1158
MslI CAYNNNNRTG 3 cut(s) 222, 803, 1341
MspA1I CMGCKG 2 cut(s) 766, 1298
MspI CCGG 3 cut(s) 46, 404, 1137
MspR9I CCNGG 5 cut(s) 404, 471, 1204, 1221, 1269
Mva1269I GAATGC 1 cut(s) 692
MvaI CCWGG 4 cut(s) 471, 1204, 1221, 1269
MvnI CGCG 1 cut(s) 766
MwoI GCNNNNNNNGC 1 cut(s) 599
NciI CCSGG 1 cut(s) 404
NdeII GATC 6 cut(s) 16, 408, 435, 492, 1047, 1245
NlaIII CATG 8 cut(s) 330, 343, 488, 493, 731, 851, 953, 1190
NlaIV GGNNCC 5 cut(s) 6, 252, 651, 1093, 1201
NmeAIII GCCGAG 1 cut(s) 526
NmuCI GTSAC 2 cut(s) 988, 1139
NsiI ATGCAT 1 cut(s) 694
NspI RCATGY 2 cut(s) 953, 1190
OliI CACNNNNGTG 1 cut(s) 222
PaeI GCATGC 1 cut(s) 1190
PagI TCATGA 1 cut(s) 489
PciSI GCTCTTC 1 cut(s) 468
PctI GAATGC 1 cut(s) 692
PdmI GAANNNNTTC 1 cut(s) 1096
PfeI GAWTC 2 cut(s) 237, 1292
PflFI GACNNNGTC 1 cut(s) 485
PfoI TCCNGGA 1 cut(s) 402
PinAI ACCGGT 1 cut(s) 1136
PkrI GCNGC 1 cut(s) 267
PleI GAGTC 2 cut(s) 617, 995
PpsI GAGTC 2 cut(s) 617, 995
PpuMI RGGWCCY 1 cut(s) 817
PsiI TTATAA 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 4 cut(s) 469, 1202, 1219, 1267
PspGI CCWGG 4 cut(s) 469, 1202, 1219, 1267
PspN4I GGNNCC 5 cut(s) 6, 252, 651, 1093, 1201
PspPI GGNCC 4 cut(s) 48, 649, 817, 973
PspPPI RGGWCCY 1 cut(s) 817
PstNI CAGNNNCTG 1 cut(s) 1027
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 1 cut(s) 856
RsaNI GTAC 1 cut(s) 855
RseI CAYNNNNRTG 3 cut(s) 222, 803, 1341
Rsr2I CGGWCCG 1 cut(s) 48
RsrII CGGWCCG 1 cut(s) 48
SacII CCGCGG 1 cut(s) 767
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 4 cut(s) 555, 663, 701, 1158
SatI GCNGC 1 cut(s) 266
Sau3AI GATC 6 cut(s) 16, 408, 435, 492, 1047, 1245
Sau96I GGNCC 4 cut(s) 48, 649, 817, 973
SchI GAGTC 2 cut(s) 618, 996
ScrFI CCNGG 5 cut(s) 404, 471, 1204, 1221, 1269
SduI GDGCHC 3 cut(s) 393, 683, 1075
SfaNI GCATC 5 cut(s) 166, 318, 529, 679, 807
SfcI CTRYAG 2 cut(s) 595, 910
Sfr303I CCGCGG 1 cut(s) 767
SgrAI CRCCGGYG 1 cut(s) 1136
SgrBI CCGCGG 1 cut(s) 767
SinI GGWCC 3 cut(s) 48, 649, 817
SmiMI CAYNNNNRTG 3 cut(s) 222, 803, 1341
SmlI CTYRAG 1 cut(s) 1144
SmoI CTYRAG 1 cut(s) 1144
SpeI ACTAGT 1 cut(s) 841
SphI GCATGC 1 cut(s) 1190
Sse9I AATT 5 cut(s) 448, 532, 668, 740, 1114
SsiI CCGC 5 cut(s) 753, 764, 766, 1296, 1320
SspMI CTAG 6 cut(s) 513, 659, 842, 870, 1077, 1274
StyD4I CCNGG 5 cut(s) 402, 469, 1202, 1219, 1267
StyI CCWWGG 2 cut(s) 232, 276
TaaI ACNGT 1 cut(s) 649
TaqI TCGA 2 cut(s) 530, 537
TasI AATT 5 cut(s) 448, 532, 668, 740, 1114
TfiI GAWTC 2 cut(s) 237, 1292
Tru1I TTAA 4 cut(s) 555, 663, 701, 1158
Tru9I TTAA 4 cut(s) 555, 663, 701, 1158
TscAI CASTG 4 cut(s) 481, 997, 1075, 1111
TseFI GTSAC 2 cut(s) 988, 1139
TseI GCWGC 1 cut(s) 265
Tsp45I GTSAC 2 cut(s) 988, 1139
TspRI CASTG 4 cut(s) 481, 997, 1075, 1111
Tth111I GACNNNGTC 1 cut(s) 485
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 3 cut(s) 48, 649, 817
XagI CCTNNNNNAGG 3 cut(s) 259, 824, 1080
XapI RAATTY 3 cut(s) 532, 668, 740
XceI RCATGY 2 cut(s) 953, 1190
XmiI GTMKAC 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 1096
XspI CTAG 6 cut(s) 513, 659, 842, 870, 1077, 1274
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.