AT4G24460

CRT-like, chloroquine-resistance transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003123
Physical Location & Seq
Forward (+)
12642844 .. 12645501
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24460.3

Sequence Viewer

Length: 1296 bp
ATGGATACCGTTCTCATGGCTACAACACCACCAATCAGATGTCTTCATGCTTCAATTCCTACGGTGTTCCGTTCGCCGGCGATTTATCAGGTCAGCTGCCGGAGCTCCCAACTGTTTTCCTACCGTTCGACGACTATGATGAGCATGTGTTTTCTCAGACGGTCCGATTTGAGATCTCGCTTTCTTAGTACTCCGAAAACCACTTCTCCGATGAGACGACCTCGCTTTTCCGTCGGTGCTTCTACGGAGGAGTCTTCAATTCCTTCTAATCGGAACCTTATCGTGGCTAACTCCGTCGTAATCGTAGCTTTAGCTGTAGCTAATCGCGTGCTCTACAAACTCGCTTTGGTTCCCATGAAACAGTACCCTTTTTTCATGGCTCAGCTCACCACTTTCGGCTACGTCTTGATTTATTTCACCATTCTGTATACGAGGCGCCGTCTAGGCATTGTGACGAATGAAATGATGGATGTACCCAAATGGAGATTCGCAATCATTGGTTTTCTCGAAGCTCTTGGTGTTGCCACTGGCATGGCTGCAGCAGCAATGCTTCCTGGACCAGTTATACCCATTTTGAATCAGACATATTTGGTGTGGCAGCTGCTTTTTGCTCTTCTTATTTTAGGCAGGAGGTTTTTGCTTAACCAAATTGCTGGTTGTCTCCTTGTGGCTGTGGGGGTTGTGGTGGCTGTTTCAAGTGGATCTGGTGCAGATACTACGCTATCTGGAATCGGTTTCTTATGGCCTGCTGTATTGGTAGCATCCGCTGCTTTTCAAGCTGGTGCATCTATCATAAAGGAATTTGTTTTCAATGATGCTGCAAAGCGTCTTGAGGGAAAGTCACTGGATATATTTGTGGTAAATTCATTTGGATCTGGATTTCAGGCTCTTTTTGTGTTCCTGTTGTTGCCTTTTCTCTCAAACTTGAAAGGCATTCCATTCGCCAGCCTTCCTTCATATCTAAAAGATGGTGCCGGATGCTTCTTCAACACTGGAGCTAAGATTTCTGGCTGTGATGGTGCCCCTATACTCCCACTGCTCTATATATCCACCAATCTCGCTTTCAACATCTCATTGCTCCATCTGGTGAAAATATCTTCAGCAATCGTTTCCTCTCTTACCATGATGCTCTCAGTGCCATTAGCGGTGTACATAATGTCAAAACCATTGCCGTATCTACCGGGAGGATCAAGCTTGAGCTCAAATTTTACGATGGGGTGTATAGTTCTTGTCCTAGGTCTTCTTCTCTACAACATTCCCACTACTCCAACTAAACAACACACCAAGACCTCCTAA

Protein Analysis

431

Amino Acids

46.68

Weight (kDa)

9.94

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 97 - 295 6.8e-29 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 435, 971, 1019
AccI GTMKAC 1 cut(s) 428
AccII CGCG 1 cut(s) 327
AciI CCGC 2 cut(s) 765, 1145
AclWI GGATC 3 cut(s) 709, 880, 1195
AcsI RAATTY 3 cut(s) 800, 862, 1204
AcuI CTGAAG 1 cut(s) 1083
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 4 cut(s) 190, 365, 474, 1151
AfiI CCNNNNNNNGG 2 cut(s) 76, 283
AgsI TTSAA 9 cut(s) 54, 258, 577, 696, 776, 811, 928, 988, 1066
AjnI CCWGG 1 cut(s) 553
AjuI GAANNNNNNNTTGG 4 cut(s) 470, 502, 1046, 1078
Alw21I GWGCWC 3 cut(s) 107, 333, 1202
Alw26I GTCTC 2 cut(s) 208, 665
AlwI GGATC 3 cut(s) 709, 880, 1195
AoxI GGCC 1 cut(s) 743
ApeKI GCWGC 8 cut(s) 96, 536, 539, 542, 598, 601, 767, 818
ApoI RAATTY 3 cut(s) 800, 862, 1204
AspA2I CCTAGG 1 cut(s) 1234
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 162, 557
AsuC2I CCSGG 1 cut(s) 1182
AsuHPI GGTGA 3 cut(s) 379, 409, 1099
AvaII GGWCC 2 cut(s) 162, 557
AvrII CCTAGG 1 cut(s) 1234
BaeGI GKGCMC 1 cut(s) 1024
BanI GGYRCC 3 cut(s) 435, 971, 1019
BanII GRGCYC 2 cut(s) 107, 1202
BbsI GAAGAC 3 cut(s) 35, 246, 1232
Bbv12I GWGCWC 3 cut(s) 107, 333, 1202
BbvI GCAGC 8 cut(s) 83, 523, 551, 554, 588, 610, 754, 805
BccI CCATC 5 cut(s) 460, 962, 1010, 1089, 1207
BceAI ACGGC 2 cut(s) 423, 1156
BcgI CGANNNNNNTGC 2 cut(s) 922, 956
BciT130I CCWGG 1 cut(s) 555
BcnI CCSGG 1 cut(s) 1182
BcoDI GTCTC 2 cut(s) 208, 665
BfaI CTAG 2 cut(s) 443, 1235
BfmI CTRYAG 2 cut(s) 315, 537
BfoI RGCGCY 1 cut(s) 439
BglI GCCNNNNNGGC 1 cut(s) 444
BglII AGATCT 1 cut(s) 173
BisI GCNGC 8 cut(s) 97, 537, 540, 543, 599, 602, 768, 819
BlnI CCTAGG 1 cut(s) 1234
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 8 cut(s) 98, 538, 541, 544, 600, 603, 769, 820
BmcAI AGTACT 1 cut(s) 190
Bme1390I CCNGG 2 cut(s) 555, 1182
Bme18I GGWCC 2 cut(s) 162, 557
BmgT120I GGNCC 2 cut(s) 162, 557
BmiI GGNNCC 5 cut(s) 275, 351, 437, 973, 1021
BmrFI CCNGG 2 cut(s) 555, 1182
BmsI GCATC 5 cut(s) 770, 794, 805, 968, 1116
BpiI GAAGAC 3 cut(s) 35, 246, 1232
BplI GAGNNNNNCTC 2 cut(s) 205, 237
BpmI CTGGAG 1 cut(s) 1014
Bpu1102I GCTNAGC 1 cut(s) 381
BpuEI CTTGAG 2 cut(s) 851, 1216
BpuMI CCSGG 1 cut(s) 1182
BsaHI GRCGYC 1 cut(s) 436
BsaJI CCNNGG 1 cut(s) 1234
BsaXI ACNNNNNCTCC 2 cut(s) 190, 220
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 283
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 4 cut(s) 532, 560, 849, 997
Bse3DI GCAATG 3 cut(s) 552, 1073, 1166
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 1 cut(s) 1234
BseGI GGATG 3 cut(s) 475, 761, 983
BseLI CCNNNNNNNGG 2 cut(s) 76, 283
BseMI GCAATG 3 cut(s) 552, 1073, 1166
BseMII CTCAG 3 cut(s) 169, 395, 1146
BseNI ACTGG 4 cut(s) 532, 560, 849, 997
BseRI GAGGAG 1 cut(s) 263
BseSI GKGCMC 1 cut(s) 1024
BseXI GCAGC 8 cut(s) 83, 523, 551, 554, 588, 610, 754, 805
BsgI GTGCAG 1 cut(s) 729
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 1 cut(s) 745
BshNI GGYRCC 3 cut(s) 435, 971, 1019
BsiHKAI GWGCWC 3 cut(s) 107, 333, 1202
BsiSI CCGG 4 cut(s) 77, 100, 975, 1181
BslI CCNNNNNNNGG 2 cut(s) 76, 283
BsmAI GTCTC 2 cut(s) 208, 665
BsmBI CGTCTC 1 cut(s) 208
BsmI GAATGC 1 cut(s) 933
BsnI GGCC 1 cut(s) 745
Bsp1286I GDGCHC 4 cut(s) 107, 333, 1024, 1202
Bsp1407I TGTACA 1 cut(s) 1149
Bsp143I GATC 4 cut(s) 173, 701, 872, 1187
Bsp1720I GCTNAGC 1 cut(s) 381
BspACI CCGC 2 cut(s) 765, 1145
BspANI GGCC 1 cut(s) 745
BspCNI CTCAG 3 cut(s) 168, 394, 1145
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 5 cut(s) 275, 351, 437, 973, 1021
BspMAI CTGCAG 1 cut(s) 541
BspPI GGATC 3 cut(s) 709, 880, 1195
BspQI GCTCTTC 1 cut(s) 618
BspT107I GGYRCC 3 cut(s) 435, 971, 1019
BsrDI GCAATG 3 cut(s) 552, 1073, 1166
BsrFI RCCGGY 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 1149
BsrI ACTGG 4 cut(s) 532, 560, 849, 997
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 1234
BssMI GATC 4 cut(s) 173, 701, 872, 1187
BssNAI GTATAC 1 cut(s) 429
BssNI GRCGYC 1 cut(s) 436
BssT1I CCWWGG 1 cut(s) 1234
Bst1107I GTATAC 1 cut(s) 429
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 6 cut(s) 10, 64, 114, 125, 162, 363
Bst6I CTCTTC 1 cut(s) 618
BstACI GRCGYC 1 cut(s) 436
BstAPI GCANNNNNTGC 1 cut(s) 767
BstAUI TGTACA 1 cut(s) 1149
BstC8I GCNNGC 4 cut(s) 78, 329, 747, 946
BstDEI CTNAG 5 cut(s) 155, 185, 381, 999, 1132
BstF5I GGATG 3 cut(s) 475, 761, 983
BstFNI CGCG 1 cut(s) 327
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 4 cut(s) 176, 704, 875, 1190
BstMAI GTCTC 2 cut(s) 208, 665
BstMBI GATC 4 cut(s) 173, 701, 872, 1187
BstMWI GCNNNNNNNGC 6 cut(s) 102, 444, 542, 767, 776, 1135
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 1 cut(s) 148
BstSCI CCNGG 2 cut(s) 553, 1180
BstSFI CTRYAG 2 cut(s) 315, 537
BstSLI GKGCMC 1 cut(s) 1024
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 8 cut(s) 83, 523, 551, 554, 588, 610, 754, 805
BstV2I GAAGAC 3 cut(s) 35, 246, 1232
BstX2I RGATCY 3 cut(s) 173, 701, 872
BstXI CCANNNNNNTGG 2 cut(s) 532, 653
BstYI RGATCY 3 cut(s) 173, 701, 872
BstZ17I GTATAC 1 cut(s) 429
BsuRI GGCC 1 cut(s) 745
BtsCI GGATG 3 cut(s) 475, 761, 983
BtsI GCAGTG 1 cut(s) 1034
BtsIMutI CAGTG 5 cut(s) 525, 842, 990, 1034, 1140
Cac8I GCNNGC 4 cut(s) 78, 329, 747, 946
CfoI GCGC 1 cut(s) 438
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 162, 557
CpoI CGGWCCG 1 cut(s) 162
CseI GACGC 1 cut(s) 815
Csp6I GTAC 4 cut(s) 189, 364, 473, 1150
CspI CGGWCCG 1 cut(s) 162
CviAII CATG 7 cut(s) 16, 47, 145, 355, 376, 532, 1123
CviQI GTAC 4 cut(s) 189, 364, 473, 1150
DdeI CTNAG 5 cut(s) 155, 185, 381, 999, 1132
DinI GGCGCC 1 cut(s) 437
DpnI GATC 4 cut(s) 175, 703, 874, 1189
DpnII GATC 4 cut(s) 173, 701, 872, 1187
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Ecl136II GAGCTC 2 cut(s) 105, 1200
Eco130I CCWWGG 1 cut(s) 1234
Eco24I GRGCYC 2 cut(s) 107, 1202
Eco47I GGWCC 2 cut(s) 162, 557
Eco53kI GAGCTC 2 cut(s) 105, 1200
Eco57I CTGAAG 1 cut(s) 1083
EcoICRI GAGCTC 2 cut(s) 105, 1200
EcoRII CCWGG 1 cut(s) 553
EcoT14I CCWWGG 1 cut(s) 1234
EcoT38I GRGCYC 2 cut(s) 107, 1202
EgeI GGCGCC 1 cut(s) 437
EheI GGCGCC 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 1234
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 7 cut(s) 19, 50, 148, 358, 379, 535, 1126
FatI CATG 7 cut(s) 15, 46, 144, 354, 375, 531, 1122
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 8 cut(s) 97, 537, 540, 543, 599, 602, 768, 819
FokI GGATG 3 cut(s) 482, 748, 990
FriOI GRGCYC 2 cut(s) 107, 1202
Fsp4HI GCNGC 8 cut(s) 97, 537, 540, 543, 599, 602, 768, 819
FspBI CTAG 2 cut(s) 443, 1235
GlaI GCGC 1 cut(s) 437
GluI GCNGC 8 cut(s) 97, 537, 540, 543, 599, 602, 768, 819
GsuI CTGGAG 1 cut(s) 1014
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 1 cut(s) 745
HapII CCGG 4 cut(s) 77, 100, 975, 1181
HgaI GACGC 1 cut(s) 815
HhaI GCGC 1 cut(s) 438
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 7 cut(s) 19, 50, 148, 358, 379, 535, 1126
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HindIII AAGCTT 1 cut(s) 1192
HinfI GANTC 4 cut(s) 251, 486, 577, 729
HpaII CCGG 4 cut(s) 77, 100, 975, 1181
HphI GGTGA 3 cut(s) 379, 409, 1099
Hpy166II GTNNAC 2 cut(s) 429, 1150
Hpy188I TCNGA 7 cut(s) 38, 158, 166, 195, 210, 273, 582
Hpy188III TCNNGA 5 cut(s) 406, 506, 726, 830, 876
Hpy8I GTNNAC 2 cut(s) 429, 1150
Hpy99I CGWCG 3 cut(s) 133, 236, 299
HpyAV CCTTC 3 cut(s) 273, 959, 963
HpyCH4III ACNGT 6 cut(s) 10, 64, 114, 125, 162, 363
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 4 cut(s) 539, 710, 785, 821
HpyF10VI GCNNNNNNNGC 6 cut(s) 102, 444, 542, 767, 776, 1135
HpyF3I CTNAG 5 cut(s) 155, 185, 381, 999, 1132
HpySE526I ACGT 1 cut(s) 402
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 7 cut(s) 19, 50, 148, 358, 379, 535, 1126
HspAI GCGC 1 cut(s) 436
KasI GGCGCC 1 cut(s) 435
KroI GCCGGC 1 cut(s) 76
KroNI GCCGGC 1 cut(s) 78
Kzo9I GATC 4 cut(s) 173, 701, 872, 1187
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 4 cut(s) 102, 110, 995, 1083
Lsp1109I GCAGC 8 cut(s) 83, 523, 551, 554, 588, 610, 754, 805
LweI GCATC 5 cut(s) 770, 794, 805, 968, 1116
MaeI CTAG 2 cut(s) 443, 1235
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 2 cut(s) 451, 840
MalI GATC 4 cut(s) 175, 703, 874, 1189
MboI GATC 4 cut(s) 173, 701, 872, 1187
MboII GAAGA 7 cut(s) 35, 246, 605, 976, 1089, 1232, 1235
MflI RGATCY 3 cut(s) 173, 701, 872
MhlI GDGCHC 4 cut(s) 107, 333, 1024, 1202
MluCI AATT 6 cut(s) 54, 258, 648, 800, 862, 1204
Mly113I GGCGCC 1 cut(s) 436
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 1292
MnlI CCTC 7 cut(s) 231, 241, 426, 624, 826, 1123, 1178
MreI CGCCGGCG 1 cut(s) 76
MroNI GCCGGC 1 cut(s) 76
MseI TTAA 1 cut(s) 642
MslI CAYNNNNRTG 1 cut(s) 530
MspA1I CMGCKG 3 cut(s) 96, 601, 767
MspI CCGG 4 cut(s) 77, 100, 975, 1181
MspR9I CCNGG 2 cut(s) 555, 1182
Mva1269I GAATGC 1 cut(s) 933
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 6 cut(s) 102, 444, 542, 767, 776, 1135
NaeI GCCGGC 1 cut(s) 78
NarI GGCGCC 1 cut(s) 436
NciI CCSGG 1 cut(s) 1182
NdeII GATC 4 cut(s) 173, 701, 872, 1187
NgoMIV GCCGGC 1 cut(s) 76
NlaIII CATG 7 cut(s) 19, 50, 148, 358, 379, 535, 1126
NlaIV GGNNCC 5 cut(s) 275, 351, 437, 973, 1021
NmuCI GTSAC 2 cut(s) 451, 840
NspI RCATGY 1 cut(s) 148
PciSI GCTCTTC 1 cut(s) 618
PctI GAATGC 1 cut(s) 933
PdiI GCCGGC 1 cut(s) 78
PfeI GAWTC 3 cut(s) 486, 577, 729
PfoI TCCNGGA 1 cut(s) 553
PkrI GCNGC 8 cut(s) 98, 538, 541, 544, 600, 603, 769, 820
PleI GAGTC 1 cut(s) 259
PluTI GGCGCC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 259
Psp124BI GAGCTC 2 cut(s) 107, 1202
Psp6I CCWGG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 5 cut(s) 275, 351, 437, 973, 1021
PspPI GGNCC 2 cut(s) 162, 557
PstI CTGCAG 1 cut(s) 541
PsuI RGATCY 3 cut(s) 173, 701, 872
PvuII CAGCTG 2 cut(s) 96, 601
RsaI GTAC 4 cut(s) 190, 365, 474, 1151
RsaNI GTAC 4 cut(s) 189, 364, 473, 1150
RseI CAYNNNNRTG 1 cut(s) 530
Rsr2I CGGWCCG 1 cut(s) 162
RsrII CGGWCCG 1 cut(s) 162
SacI GAGCTC 2 cut(s) 107, 1202
SapI GCTCTTC 1 cut(s) 618
SaqAI TTAA 1 cut(s) 642
SatI GCNGC 8 cut(s) 97, 537, 540, 543, 599, 602, 768, 819
Sau3AI GATC 4 cut(s) 173, 701, 872, 1187
Sau96I GGNCC 2 cut(s) 162, 557
ScaI AGTACT 1 cut(s) 190
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 2 cut(s) 555, 1182
SduI GDGCHC 4 cut(s) 107, 333, 1024, 1202
SfaNI GCATC 5 cut(s) 770, 794, 805, 968, 1116
SfcI CTRYAG 2 cut(s) 315, 537
SfoI GGCGCC 1 cut(s) 437
SgrAI CRCCGGYG 1 cut(s) 76
SinI GGWCC 2 cut(s) 162, 557
SmiMI CAYNNNNRTG 1 cut(s) 530
SmlI CTYRAG 2 cut(s) 830, 1195
SmoI CTYRAG 2 cut(s) 830, 1195
Sse9I AATT 6 cut(s) 54, 258, 648, 800, 862, 1204
SsiI CCGC 2 cut(s) 765, 1145
SspDI GGCGCC 1 cut(s) 435
SspMI CTAG 2 cut(s) 443, 1235
SstI GAGCTC 2 cut(s) 107, 1202
StyD4I CCNGG 2 cut(s) 553, 1180
StyI CCWWGG 1 cut(s) 1234
TaaI ACNGT 6 cut(s) 10, 64, 114, 125, 162, 363
TaiI ACGT 1 cut(s) 405
TaqI TCGA 2 cut(s) 128, 507
TasI AATT 6 cut(s) 54, 258, 648, 800, 862, 1204
TatI WGTACW 2 cut(s) 188, 1149
TfiI GAWTC 3 cut(s) 486, 577, 729
Tru1I TTAA 1 cut(s) 642
Tru9I TTAA 1 cut(s) 642
TscAI CASTG 5 cut(s) 532, 849, 997, 1041, 1140
TseFI GTSAC 2 cut(s) 451, 840
TseI GCWGC 8 cut(s) 96, 536, 539, 542, 598, 601, 767, 818
Tsp45I GTSAC 2 cut(s) 451, 840
TspDTI ATGAA 6 cut(s) 35, 364, 371, 474, 855, 945
TspGWI ACGGA 4 cut(s) 59, 220, 260, 283
TspRI CASTG 5 cut(s) 532, 849, 997, 1041, 1140
VpaK11BI GGWCC 2 cut(s) 162, 557
XapI RAATTY 3 cut(s) 800, 862, 1204
XceI RCATGY 1 cut(s) 148
XmaJI CCTAGG 1 cut(s) 1234
XmiI GTMKAC 1 cut(s) 428
XspI CTAG 2 cut(s) 443, 1235
ZrmI AGTACT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.