MD08G1202700.v1.1

pollen tube development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003123
Physical Location & Seq
Reverse (-)
26216701 .. 26218681
1981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1202700.v1.1.491

Sequence Viewer

Length: 420 bp
ATGCAGCGACCCTTGCTCACTTATCACTGTGCTCAGGCCACACAGCGCGCCACTTTTCAGGCTCTTTTTGTTCTTCTGTTTCTACCTTTCTTGTCAAACCTGAGAGGCATACCATTTGCGCAACTTCCTTCATATCTAAAAGATGGTTGCAATGGGGCTCCGTTACTACCATTGCTGTATATAGCTACCAATCTGTTTTTCAACATTTCATTGCTGAATGTAGTAAAGATTTCCTCTGCGGTTGTTGCTTCTCTCATCGTGATGTTGTCAGTGCCAATATCAATTTATGTTCTTTCCCTTCCCTTGCCGTATATGGAAGGAGGTTCAACTTTGAGCCCCTTTTTCCTCTTCGGTAGTGCGGTTCTCGTGTTGGGTCTTATTCTGTACAGCATACCCCAGCCTGCCAAGGATCTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Apt1 DUF2405 Fmp27_GFWDK

Protein Analysis

140

Amino Acids

15.15

Weight (kDa)

9.06

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 45 - 129 8.4e-07 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 120
AccII CGCG 1 cut(s) 48
AciI CCGC 2 cut(s) 239, 359
AclWI GGATC 1 cut(s) 417
AfaI GTAC 1 cut(s) 386
AgsI TTSAA 2 cut(s) 202, 327
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AluBI AGCT 1 cut(s) 185
AluI AGCT 1 cut(s) 185
Alw21I GWGCWC 1 cut(s) 34
AlwI GGATC 1 cut(s) 417
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 4
AspLEI GCGC 3 cut(s) 48, 50, 121
BanII GRGCYC 2 cut(s) 160, 338
BauI CACGAG 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 34
BbvI GCAGC 1 cut(s) 16
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 292
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
BmiI GGNNCC 1 cut(s) 159
Bpu10I CCTNAGC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 405
Bse3DI GCAATG 3 cut(s) 157, 170, 209
BseDI CCNNGG 1 cut(s) 405
BseMI GCAATG 3 cut(s) 157, 170, 209
BseMII CTCAG 2 cut(s) 47, 92
BsePI GCGCGC 1 cut(s) 46
BseXI GCAGC 1 cut(s) 16
BseYI CCCAGC 1 cut(s) 396
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 34
BsnI GGCC 1 cut(s) 38
Bsp1286I GDGCHC 3 cut(s) 34, 160, 338
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 1 cut(s) 409
BspACI CCGC 2 cut(s) 239, 359
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 2 cut(s) 46, 93
BspFNI CGCG 1 cut(s) 48
BspLI GGNNCC 1 cut(s) 159
BspPI GGATC 1 cut(s) 417
BsrDI GCAATG 3 cut(s) 157, 170, 209
BsrGI TGTACA 1 cut(s) 384
BssECI CCNNGG 1 cut(s) 405
BssHII GCGCGC 1 cut(s) 46
BssMI GATC 1 cut(s) 409
BssSI CACGAG 1 cut(s) 365
BssT1I CCWWGG 1 cut(s) 405
Bst2BI CACGAG 1 cut(s) 365
Bst4CI ACNGT 1 cut(s) 29
Bst6I CTCTTC 1 cut(s) 353
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 2 cut(s) 48, 402
BstDEI CTNAG 2 cut(s) 33, 101
BstFNI CGCG 1 cut(s) 48
BstHHI GCGC 3 cut(s) 48, 50, 121
BstKTI GATC 1 cut(s) 412
BstMBI GATC 1 cut(s) 409
BstMWI GCNNNNNNNGC 2 cut(s) 13, 245
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
BsuRI GGCC 1 cut(s) 38
BtsIMutI CAGTG 2 cut(s) 25, 276
Cac8I GCNNGC 2 cut(s) 48, 402
CfoI GCGC 3 cut(s) 48, 50, 121
Csp6I GTAC 1 cut(s) 385
CviJI RGCY 6 cut(s) 38, 62, 158, 185, 336, 400
CviKI_1 RGCY 6 cut(s) 38, 62, 158, 185, 336, 400
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 2 cut(s) 33, 101
DpnI GATC 1 cut(s) 411
DpnII GATC 1 cut(s) 409
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 2 cut(s) 160, 338
EcoT14I CCWWGG 1 cut(s) 405
EcoT38I GRGCYC 2 cut(s) 160, 338
ErhI CCWWGG 1 cut(s) 405
FaiI YATR 8 cut(s) 110, 133, 180, 182, 288, 312, 314, 392
Fnu4HI GCNGC 1 cut(s) 5
FriOI GRGCYC 2 cut(s) 160, 338
Fsp4HI GCNGC 1 cut(s) 5
FspI TGCGCA 1 cut(s) 120
GlaI GCGC 3 cut(s) 47, 49, 120
GluI GCNGC 1 cut(s) 5
GsaI CCCAGC 1 cut(s) 400
HaeIII GGCC 1 cut(s) 38
HhaI GCGC 3 cut(s) 48, 50, 121
Hin6I GCGC 3 cut(s) 46, 48, 119
HinP1I GCGC 3 cut(s) 46, 48, 119
Hpy188III TCNNGA 1 cut(s) 259
HpyAV CCTTC 3 cut(s) 138, 308, 311
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4V TGCA 2 cut(s) 4, 150
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 245
HpyF3I CTNAG 2 cut(s) 33, 101
HspAI GCGC 3 cut(s) 46, 48, 119
Kzo9I GATC 1 cut(s) 409
LmnI GCTCC 1 cut(s) 163
LpnPI CCDG 5 cut(s) 20, 44, 113, 410, 414
Lsp1109I GCAGC 1 cut(s) 16
MaeIII GTNAC 1 cut(s) 162
MalI GATC 1 cut(s) 411
MboI GATC 1 cut(s) 409
MboII GAAGA 2 cut(s) 65, 340
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 3 cut(s) 34, 160, 338
MluCI AATT 1 cut(s) 282
MnlI CCTC 4 cut(s) 98, 244, 314, 356
MslI CAYNNNNRTG 1 cut(s) 260
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 2 cut(s) 13, 245
NdeII GATC 1 cut(s) 409
NlaIV GGNNCC 1 cut(s) 159
NsbI TGCGCA 1 cut(s) 120
PauI GCGCGC 1 cut(s) 46
PkrI GCNGC 1 cut(s) 6
PspFI CCCAGC 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 159
PsuI RGATCY 1 cut(s) 409
PteI GCGCGC 1 cut(s) 46
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 260
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 1 cut(s) 409
SduI GDGCHC 3 cut(s) 34, 160, 338
SetI ASST 4 cut(s) 88, 102, 187, 325
SmiMI CAYNNNNRTG 1 cut(s) 260
Sse9I AATT 1 cut(s) 282
SsiI CCGC 2 cut(s) 239, 359
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 1 cut(s) 29
TasI AATT 1 cut(s) 282
TatI WGTACW 1 cut(s) 384
TscAI CASTG 2 cut(s) 32, 276
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 2 cut(s) 120, 198
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 2 cut(s) 32, 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.