RchiOBHm_Chr7g0234941

CRT-like, chloroquine-resistance transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003123
Physical Location & Seq
Forward (+)
59639458 .. 59645256
5799 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21055

Sequence Viewer

Length: 1386 bp
ATGATGGGTGCTTCCTCTACGTTGTCGTTTCACAAGTTCCTCTCCGTCCCCCCTTCTCTCTCTCACCTCAAAAATGGCGGGACTTCCTTCTCCTCCTCCTCTCCGACCTCAATGCCACTCCTCCATAGGCCTCGCCCTTCTCTCCTCCGCCTCCACACTCCAAACGCACCGTTTCGCCTCCCCTCCGATCGCAAACCCACCGCCCGCAATGTCAAGCTCCACGCTTCACTCCCAAACCCTCCCCCCTCCTCCGCGATCTCAAACGGCGTCGTTTTCAGCTCCGTGATTACCATAGCCCTGGCCGTCGCCAACCGCGTTCTCTACAAGCTCGCACTCGTCCCCATGAAGAACCACCCCTTCTTTCTGGCCCAATTCATAACCTTTGGTTATGTGGTGATATATTTCTCAATATTGTACATACGGTACCGTTCTGGGATTGTGACTGACGAAATGCTAAGGCTTCCGAAATCGCGGTTCGTGGCTATCGGAGCCTTAGAGGCCCTTGGAGTCGCTTCTGGGATGGCTGCTGCAGCCATGCTTCCCGGACCGGCCATACCCATATTGAGTCAGACGTTTTTGGTGTGGCAGCTTGGTTTTTCTTCTCTTATCTTGGGAAGATCATACAGAATCAATCAGATTGTTGGCTGTATCCTCGTAGCTGCAGGTGTAGCGCTAGCTGTTGCTAGTGGGAAAGATTCTGGCCAGATGCTATCTGGAGTTGAGTCTGTGTGGCCATTACTAATGATTTCTTCAAGTGCTTTTCAAGCTGGTGCATCTATAATCAAGGAATTCGTCTTTGTTGATGCTGCGGTCCACTTAAAGGGAAAATCACTTGACATATTTGTTGTCAACTCTTTTGGATCTGGATTTCAGGCTCTTTTTGTACTTCTATTTCTACCTTTCTTATCAAATATGAGAGGCATACCATTCGCCCAACTTCCTACATATCTAAAAGATGGTGCTGGTTGCTTTCTCAATATGGGAGCTACTGCATCAGGCAAGCTGTCTTATCTATCTTCAATTACTTTCTGTAATTTTGTAAATACATTGATACCGGAGTTATCTGGGATTCTGGGACATGCTTTCAGTAATAATGATATGTGTTGTAATGGGGCTCCATTGCTTCCACTGCTTTACATAGCTGCCAATCTGTTGTTCAACATAAGCTTGCTGAATGTAGTAAAGATCTCCTCTGCGGTTGTTGCTTCTCTTATTGTGATGTTGTCAGTGCCAATCTCGATTTATGTTCTTTCCCTTCCATTGCCATATCTCCGAGAGGGTGCAACTTTGAGCCCCTTCTTCCTCTTTGGTAGTGCGGTTCTTGTCTTGGGTCTTATTTTGTACAGCATACCCCAACCTGCCAAGGATGTTTCTGAAATTAGTTGA

Protein Analysis

461

Amino Acids

49.18

Weight (kDa)

9.75

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 90 - 448 1.2e-38 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 653
Acc36I ACCTGC 2 cut(s) 653, 1366
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 1 cut(s) 423
AccII CGCG 3 cut(s) 254, 315, 472
AclWI GGATC 1 cut(s) 868
AcoI YGGCCR 4 cut(s) 300, 549, 700, 731
AcsI RAATTY 1 cut(s) 788
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 4 cut(s) 416, 425, 885, 1343
AfeI AGCGCT 1 cut(s) 672
AfiI CCNNNNNNNGG 1 cut(s) 820
AgsI TTSAA 4 cut(s) 753, 764, 1020, 1159
AjnI CCWGG 1 cut(s) 297
AjuI GAANNNNNNNTTGG 2 cut(s) 1139, 1171
AlwI GGATC 1 cut(s) 868
Aor51HI AGCGCT 1 cut(s) 672
AoxI GGCC 7 cut(s) 128, 300, 366, 498, 549, 700, 731
ApeKI GCWGC 7 cut(s) 524, 527, 530, 586, 659, 806, 1142
ApoI RAATTY 1 cut(s) 788
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 673
AspS9I GGNCC 4 cut(s) 367, 499, 545, 811
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 56, 406
AsuNHI GCTAGC 1 cut(s) 673
AvaII GGWCC 2 cut(s) 545, 811
BaeI ACNNNNGTAYC 2 cut(s) 1043, 1076
BalI TGGCCA 2 cut(s) 702, 733
BanI GGYRCC 1 cut(s) 423
BanII GRGCYC 2 cut(s) 1117, 1295
BbvI GCAGC 7 cut(s) 511, 514, 542, 598, 646, 793, 1129
BccI CCATC 2 cut(s) 514, 950
BceAI ACGGC 2 cut(s) 280, 287
BcgI CGANNNNNNTGC 4 cut(s) 94, 128, 910, 944
BciT130I CCWGG 1 cut(s) 299
BciVI GTATCC 1 cut(s) 659
BcnI CCSGG 1 cut(s) 543
BfaI CTAG 2 cut(s) 674, 684
BfmI CTRYAG 2 cut(s) 528, 660
BfoI RGCGCY 1 cut(s) 674
BfuAI ACCTGC 2 cut(s) 653, 1366
BfuI GTATCC 1 cut(s) 659
BglI GCCNNNNNGGC 1 cut(s) 497
BglII AGATCT 1 cut(s) 1185
BisI GCNGC 7 cut(s) 525, 528, 531, 587, 660, 807, 1143
BlsI GCNGC 7 cut(s) 526, 529, 532, 588, 661, 808, 1144
Bme1390I CCNGG 2 cut(s) 299, 543
Bme18I GGWCC 2 cut(s) 545, 811
BmgT120I GGNCC 4 cut(s) 367, 499, 545, 811
BmiI GGNNCC 3 cut(s) 425, 490, 1116
BmrFI CCNGG 2 cut(s) 299, 543
BmsI GCATC 4 cut(s) 696, 782, 793, 1001
BmtI GCTAGC 1 cut(s) 677
BpmI CTGGAG 1 cut(s) 735
Bpu10I CCTNAGC 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 297, 502, 1362
BsaWI WCCGGW 1 cut(s) 1054
Bsc4I CCNNNNNNNGG 1 cut(s) 820
Bse118I RCCGGY 1 cut(s) 547
Bse3DI GCAATG 3 cut(s) 214, 1118, 1259
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 3 cut(s) 297, 502, 1362
BseGI GGATG 2 cut(s) 525, 1372
BseLI CCNNNNNNNGG 1 cut(s) 820
BseMI GCAATG 3 cut(s) 214, 1118, 1259
BseRI GAGGAG 7 cut(s) 82, 85, 88, 110, 134, 238, 1180
BseXI GCAGC 7 cut(s) 511, 514, 542, 598, 646, 793, 1129
Bsh1236I CGCG 3 cut(s) 254, 315, 472
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 7 cut(s) 130, 302, 368, 500, 551, 702, 733
BshNI GGYRCC 1 cut(s) 423
BsiEI CGRYCG 1 cut(s) 190
BsiSI CCGG 3 cut(s) 543, 548, 1055
BslFI GGGAC 4 cut(s) 32, 94, 323, 1089
BslI CCNNNNNNNGG 1 cut(s) 820
BsmFI GGGAC 4 cut(s) 32, 94, 323, 1089
BsnI GGCC 7 cut(s) 130, 302, 368, 500, 551, 702, 733
Bsp1286I GDGCHC 2 cut(s) 1117, 1295
Bsp1407I TGTACA 2 cut(s) 414, 1341
Bsp143I GATC 5 cut(s) 187, 255, 617, 860, 1185
BspANI GGCC 7 cut(s) 130, 302, 368, 500, 551, 702, 733
BspFNI CGCG 3 cut(s) 254, 315, 472
BspLI GGNNCC 3 cut(s) 425, 490, 1116
BspMAI CTGCAG 2 cut(s) 532, 664
BspMI ACCTGC 2 cut(s) 653, 1366
BspOI GCTAGC 1 cut(s) 677
BspPI GGATC 1 cut(s) 868
BspT107I GGYRCC 1 cut(s) 423
BsrDI GCAATG 3 cut(s) 214, 1118, 1259
BsrFI RCCGGY 1 cut(s) 547
BsrGI TGTACA 2 cut(s) 414, 1341
BssAI RCCGGY 1 cut(s) 547
BssECI CCNNGG 3 cut(s) 297, 502, 1362
BssMI GATC 5 cut(s) 187, 255, 617, 860, 1185
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 2 cut(s) 502, 1362
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 3 cut(s) 171, 423, 428
BstACI GRCGYC 1 cut(s) 267
BstAUI TGTACA 2 cut(s) 414, 1341
BstC8I GCNNGC 5 cut(s) 205, 330, 675, 1001, 1169
BstDEI CTNAG 2 cut(s) 455, 493
BstF5I GGATG 2 cut(s) 525, 1372
BstFNI CGCG 3 cut(s) 254, 315, 472
BstH2I RGCGCY 1 cut(s) 674
BstHHI GCGC 1 cut(s) 673
BstKTI GATC 5 cut(s) 190, 258, 620, 863, 1188
BstMBI GATC 5 cut(s) 187, 255, 617, 860, 1185
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 7 cut(s) 488, 497, 530, 668, 764, 1129, 1202
BstNI CCWGG 1 cut(s) 299
BstNSI RCATGY 1 cut(s) 1082
BstSCI CCNGG 2 cut(s) 297, 541
BstSFI CTRYAG 2 cut(s) 528, 660
BstUI CGCG 3 cut(s) 254, 315, 472
BstV1I GCAGC 7 cut(s) 511, 514, 542, 598, 646, 793, 1129
BstX2I RGATCY 2 cut(s) 860, 1185
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 2 cut(s) 860, 1185
BsuI GTATCC 1 cut(s) 659
BsuRI GGCC 7 cut(s) 130, 302, 368, 500, 551, 702, 733
BtsCI GGATG 2 cut(s) 525, 1372
BtsI GCAGTG 1 cut(s) 1127
BtsIMutI CAGTG 2 cut(s) 1127, 1233
BveI ACCTGC 2 cut(s) 653, 1366
Cac8I GCNNGC 5 cut(s) 205, 330, 675, 1001, 1169
CfoI GCGC 1 cut(s) 673
Cfr10I RCCGGY 1 cut(s) 547
Cfr13I GGNCC 4 cut(s) 367, 499, 545, 811
CpoI CGGWCCG 1 cut(s) 545
CseI GACGC 1 cut(s) 256
Csp6I GTAC 4 cut(s) 415, 424, 884, 1342
CspI CGGWCCG 1 cut(s) 545
CviAII CATG 3 cut(s) 343, 535, 1079
CviQI GTAC 4 cut(s) 415, 424, 884, 1342
DdeI CTNAG 2 cut(s) 455, 493
DpnI GATC 5 cut(s) 189, 257, 619, 862, 1187
DpnII GATC 5 cut(s) 187, 255, 617, 860, 1185
EaeI YGGCCR 4 cut(s) 300, 549, 700, 731
EciI GGCGGA 1 cut(s) 137
Eco130I CCWWGG 2 cut(s) 502, 1362
Eco147I AGGCCT 1 cut(s) 130
Eco24I GRGCYC 2 cut(s) 1117, 1295
Eco47I GGWCC 2 cut(s) 545, 811
Eco47III AGCGCT 1 cut(s) 672
EcoO109I RGGNCCY 1 cut(s) 499
EcoRI GAATTC 1 cut(s) 788
EcoRII CCWGG 1 cut(s) 297
EcoT14I CCWWGG 2 cut(s) 502, 1362
EcoT38I GRGCYC 2 cut(s) 1117, 1295
ErhI CCWWGG 2 cut(s) 502, 1362
FaeI CATG 3 cut(s) 346, 538, 1082
FaqI GGGAC 4 cut(s) 32, 94, 323, 1089
FatI CATG 3 cut(s) 342, 534, 1078
FauI CCCGC 2 cut(s) 71, 212
Fnu4HI GCNGC 7 cut(s) 525, 528, 531, 587, 660, 807, 1143
FokI GGATG 2 cut(s) 532, 1379
FriOI GRGCYC 2 cut(s) 1117, 1295
Fsp4HI GCNGC 7 cut(s) 525, 528, 531, 587, 660, 807, 1143
FspBI CTAG 2 cut(s) 674, 684
GlaI GCGC 1 cut(s) 672
GluI GCNGC 7 cut(s) 525, 528, 531, 587, 660, 807, 1143
GsuI CTGGAG 1 cut(s) 735
HaeII RGCGCY 1 cut(s) 674
HaeIII GGCC 7 cut(s) 130, 302, 368, 500, 551, 702, 733
HapII CCGG 3 cut(s) 543, 548, 1055
HgaI GACGC 1 cut(s) 256
HhaI GCGC 1 cut(s) 673
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 3 cut(s) 346, 538, 1082
Hin6I GCGC 1 cut(s) 671
HinP1I GCGC 1 cut(s) 671
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 1 cut(s) 1165
HinfI GANTC 6 cut(s) 507, 565, 627, 695, 722, 1069
HpaII CCGG 3 cut(s) 543, 548, 1055
HphI GGTGA 2 cut(s) 56, 406
Hpy166II GTNNAC 2 cut(s) 814, 850
Hpy188I TCNGA 8 cut(s) 105, 187, 465, 488, 570, 636, 1274, 1375
Hpy188III TCNNGA 3 cut(s) 714, 864, 1237
Hpy8I GTNNAC 2 cut(s) 814, 850
Hpy99I CGWCG 2 cut(s) 272, 308
HpyAV CCTTC 6 cut(s) 63, 97, 147, 367, 1265, 1306
HpyCH4III ACNGT 3 cut(s) 171, 423, 428
HpyCH4IV ACGT 2 cut(s) 20, 572
HpyCH4V TGCA 5 cut(s) 530, 662, 773, 992, 1283
HpyF10VI GCNNNNNNNGC 7 cut(s) 488, 497, 530, 668, 764, 1129, 1202
HpyF3I CTNAG 2 cut(s) 455, 493
HpySE526I ACGT 2 cut(s) 20, 572
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 3 cut(s) 346, 538, 1082
HspAI GCGC 1 cut(s) 671
KpnI GGTACC 1 cut(s) 427
Kzo9I GATC 5 cut(s) 187, 255, 617, 860, 1185
LmnI GCTCC 5 cut(s) 222, 284, 488, 983, 1120
Lsp1109I GCAGC 7 cut(s) 511, 514, 542, 598, 646, 793, 1129
LweI GCATC 4 cut(s) 696, 782, 793, 1001
MaeI CTAG 2 cut(s) 674, 684
MaeII ACGT 2 cut(s) 20, 572
MaeIII GTNAC 1 cut(s) 439
MalI GATC 5 cut(s) 189, 257, 619, 862, 1187
MboI GATC 5 cut(s) 187, 255, 617, 860, 1185
MboII GAAGA 6 cut(s) 358, 591, 627, 741, 1008, 1291
MflI RGATCY 2 cut(s) 860, 1185
MhlI GDGCHC 2 cut(s) 1117, 1295
MlsI TGGCCA 2 cut(s) 702, 733
MluCI AATT 5 cut(s) 371, 788, 1020, 1033, 1377
MluNI TGGCCA 2 cut(s) 702, 733
MlyI GAGTC 3 cut(s) 516, 574, 731
MmeI TCCRAC 1 cut(s) 128
Mox20I TGGCCA 2 cut(s) 702, 733
MscI TGGCCA 2 cut(s) 702, 733
MseI TTAA 1 cut(s) 818
Msp20I TGGCCA 2 cut(s) 702, 733
MspI CCGG 3 cut(s) 543, 548, 1055
MspR9I CCNGG 2 cut(s) 299, 543
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 3 cut(s) 254, 315, 472
MwoI GCNNNNNNNGC 7 cut(s) 488, 497, 530, 668, 764, 1129, 1202
NciI CCSGG 1 cut(s) 543
NdeII GATC 5 cut(s) 187, 255, 617, 860, 1185
NheI GCTAGC 1 cut(s) 673
NlaIII CATG 3 cut(s) 346, 538, 1082
NlaIV GGNNCC 3 cut(s) 425, 490, 1116
NmuCI GTSAC 1 cut(s) 439
NspI RCATGY 1 cut(s) 1082
PaqCI CACCTGC 1 cut(s) 653
PceI AGGCCT 1 cut(s) 130
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfeI GAWTC 3 cut(s) 627, 695, 1069
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 7 cut(s) 526, 529, 532, 588, 661, 808, 1144
Ple19I CGATCG 1 cut(s) 190
PleI GAGTC 3 cut(s) 515, 573, 730
PpsI GAGTC 3 cut(s) 515, 573, 730
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 425, 490, 1116
PspPI GGNCC 4 cut(s) 367, 499, 545, 811
PstI CTGCAG 2 cut(s) 532, 664
PsuI RGATCY 2 cut(s) 860, 1185
PvuI CGATCG 1 cut(s) 190
RsaI GTAC 4 cut(s) 416, 425, 885, 1343
RsaNI GTAC 4 cut(s) 415, 424, 884, 1342
Rsr2I CGGWCCG 1 cut(s) 545
RsrII CGGWCCG 1 cut(s) 545
SaqAI TTAA 1 cut(s) 818
SatI GCNGC 7 cut(s) 525, 528, 531, 587, 660, 807, 1143
Sau3AI GATC 5 cut(s) 187, 255, 617, 860, 1185
Sau96I GGNCC 4 cut(s) 367, 499, 545, 811
SchI GAGTC 3 cut(s) 516, 574, 731
ScrFI CCNGG 2 cut(s) 299, 543
SduI GDGCHC 2 cut(s) 1117, 1295
SfaNI GCATC 4 cut(s) 696, 782, 793, 1001
SfcI CTRYAG 2 cut(s) 528, 660
SinI GGWCC 2 cut(s) 545, 811
Sse9I AATT 5 cut(s) 371, 788, 1020, 1033, 1377
SseBI AGGCCT 1 cut(s) 130
SspI AATATT 1 cut(s) 411
SspMI CTAG 2 cut(s) 674, 684
StuI AGGCCT 1 cut(s) 130
StyD4I CCNGG 2 cut(s) 297, 541
StyI CCWWGG 2 cut(s) 502, 1362
TaaI ACNGT 3 cut(s) 171, 423, 428
TaiI ACGT 2 cut(s) 23, 575
TaqI TCGA 1 cut(s) 1238
TasI AATT 5 cut(s) 371, 788, 1020, 1033, 1377
TatI WGTACW 3 cut(s) 414, 883, 1341
TfiI GAWTC 3 cut(s) 627, 695, 1069
Tru1I TTAA 1 cut(s) 818
Tru9I TTAA 1 cut(s) 818
TscAI CASTG 2 cut(s) 1134, 1233
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 7 cut(s) 524, 527, 530, 586, 659, 806, 1142
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 2 cut(s) 359, 364
TspGWI ACGGA 2 cut(s) 34, 271
TspRI CASTG 2 cut(s) 1134, 1233
VpaK11BI GGWCC 2 cut(s) 545, 811
XapI RAATTY 1 cut(s) 788
XceI RCATGY 1 cut(s) 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XspI CTAG 2 cut(s) 674, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.