Rw7G036700

CRT-like, chloroquine-resistance transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003123
Physical Location & Seq
Reverse (-)
55474004 .. 55488377
14374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G036700.1

Sequence Viewer

Length: 1284 bp
ATGATGGGTGCTTCCTCTACGTTGTCGTTTCACAAGTTCCTCTCCGTCCCCCCTTCTCTCTCTCACCTCAAAAATGGCGGGACTTCCTTCCCTTCCTCCTCTCCGACCTCAATGCCACTCCTCCATAGGCCTCGCCCTTCTCTCCTCCGCCTCCACACTCCAAACGCACCGTTTCGCCTCCCCTCCGATCGCAAACCCGCCGCCCGCAATGTCAAGCTCCACGCTTCACTCCCAAACCCTCCCCCCTCCTCCTCCGCGATCTCAAACGGCGTCGTTTTCAGCTCCGTGTTGACCATAGCCCTGGCCGTCGCCAACCGCGTTCTCTACAAGCTCGCACTCGTCCCCATGAAGAACCACCCCTTCTTTCTGGCCCAATTCATAACCTTTGGTTATGTGGTGATATATTTCTCAATATTGTACATACGGTACCGTTCTGGGATTGTGACTGACGAAATGCTAAGGCTTCCGAAATTGCGGTTCGTGGCCATCGGGGCCTTGGAGGCCCTTGGAGTCGCTTCTGGGATGGCTGCTGCAGCCATGCTTCCCGGACCGGCCATACCCATATTGAGTCAGACGTTTTTGGTGTGGCAGCTTGGTTTTTCTTCTCTTATCTTGGGAAGATCATACAGAATCAATCAGATTGTTGGCTGTATCCTCGTAGCTGCAGGTGTAGCGCTAGCTGTTGCTAGCGGGAACGATTCTGGCCAGATGCTATCTGGAGTTGAGTCTGTGTGGCCATTACTAATGATTTCTTCAAGTGCTTTTCAAGCTGGTGCATCTATAATCAAGGAATTTGTCTTTGTTGATGCTGCAGCCCACTTAAAGGGAAAATCACTTGACATATTTGTTGTCAACTCTTTCGGATCTGGATTTCAGGCTCTTTTTGTACTTCTATTTCTACCTTTCTTATCAAATATGAGAGGCATACCATTCGCCCAACTTCCTGCATATCTAAAAGATGGTGCTGGTTGCTTTCTGAATATGGGAGCTGCTGCATCAGGTTGTAATGGGGCTCCATTGCTTCCGCTGCTTTACATAGCTGCCAATCTGTTGTTCAACATAAGCTTGCTGAATGTAGTAAAGATCTCCTCCGCGGTTGTTGCTTCTCTCATTGTGATGTTGTCAGTGCCAATCTCGATTTATGTTCTTTCCCTTCCATTGCCATATCTCCAAGAGGGTGCAACTTTGAGCCCTTTTTTCCTCTTTGGTAGTGCAATTCTTGTCTTGGGTCTTATTCTGTACAGCATACCCCAGCCTGCCAAGGATGTTGCTAAAATTAATTGA

Protein Analysis

427

Amino Acids

45.33

Weight (kDa)

9.98

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 92 - 414 3.3e-37 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 656
Acc36I ACCTGC 1 cut(s) 656
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 426
AccII CGCG 3 cut(s) 257, 318, 1094
AclWI GGATC 1 cut(s) 871
AcoI YGGCCR 5 cut(s) 303, 483, 552, 703, 734
AcsI RAATTY 1 cut(s) 791
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 4 cut(s) 419, 428, 888, 1241
AfeI AGCGCT 1 cut(s) 675
AfiI CCNNNNNNNGG 1 cut(s) 823
AgsI TTSAA 3 cut(s) 756, 767, 1057
AjnI CCWGG 1 cut(s) 300
AjuI GAANNNNNNNTTGG 2 cut(s) 1037, 1069
AlwI GGATC 1 cut(s) 871
Aor51HI AGCGCT 1 cut(s) 675
AoxI GGCC 9 cut(s) 128, 303, 369, 483, 492, 501, 552, 703, 734
ApoI RAATTY 1 cut(s) 791
AseI ATTAAT 1 cut(s) 1278
Asp718I GGTACC 1 cut(s) 426
AspLEI GCGC 1 cut(s) 676
AspS9I GGNCC 4 cut(s) 370, 492, 502, 548
AsuC2I CCSGG 1 cut(s) 546
AsuHPI GGTGA 2 cut(s) 56, 409
AsuNHI GCTAGC 2 cut(s) 676, 686
AvaII GGWCC 1 cut(s) 548
BalI TGGCCA 3 cut(s) 485, 705, 736
BanI GGYRCC 1 cut(s) 426
BanII GRGCYC 2 cut(s) 1015, 1193
BccI CCATC 3 cut(s) 494, 517, 953
BceAI ACGGC 2 cut(s) 283, 290
BcgI CGANNNNNNTGC 4 cut(s) 94, 128, 913, 947
BciT130I CCWGG 1 cut(s) 302
BciVI GTATCC 1 cut(s) 662
BcnI CCSGG 1 cut(s) 546
BfaI CTAG 2 cut(s) 677, 687
BfmI CTRYAG 3 cut(s) 531, 663, 810
BfoI RGCGCY 1 cut(s) 677
BfuAI ACCTGC 1 cut(s) 656
BfuI GTATCC 1 cut(s) 662
BglI GCCNNNNNGGC 2 cut(s) 491, 500
BglII AGATCT 1 cut(s) 1083
Bme1390I CCNGG 2 cut(s) 302, 546
Bme18I GGWCC 1 cut(s) 548
BmgT120I GGNCC 4 cut(s) 370, 492, 502, 548
BmiI GGNNCC 3 cut(s) 428, 493, 1014
BmrFI CCNGG 2 cut(s) 302, 546
BmsI GCATC 4 cut(s) 699, 785, 796, 1004
BmtI GCTAGC 2 cut(s) 680, 690
BpmI CTGGAG 1 cut(s) 738
Bpu10I CCTNAGC 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 5 cut(s) 300, 495, 505, 1092, 1260
Bsc4I CCNNNNNNNGG 1 cut(s) 823
Bse118I RCCGGY 1 cut(s) 550
Bse3DI GCAATG 3 cut(s) 214, 1016, 1157
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 5 cut(s) 300, 495, 505, 1092, 1260
BseGI GGATG 2 cut(s) 528, 1270
BseLI CCNNNNNNNGG 1 cut(s) 823
BseMI GCAATG 3 cut(s) 214, 1016, 1157
BseRI GAGGAG 6 cut(s) 88, 110, 134, 238, 241, 1078
BseYI CCCAGC 1 cut(s) 1251
Bsh1236I CGCG 3 cut(s) 257, 318, 1094
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 9 cut(s) 130, 305, 371, 485, 494, 503, 554, 705, 736
BshNI GGYRCC 1 cut(s) 426
BsiEI CGRYCG 1 cut(s) 190
BsiSI CCGG 2 cut(s) 546, 551
BslFI GGGAC 3 cut(s) 32, 94, 326
BslI CCNNNNNNNGG 1 cut(s) 823
BsmFI GGGAC 3 cut(s) 32, 94, 326
BsnI GGCC 9 cut(s) 130, 305, 371, 485, 494, 503, 554, 705, 736
Bsp1286I GDGCHC 2 cut(s) 1015, 1193
Bsp1407I TGTACA 2 cut(s) 417, 1239
Bsp143I GATC 5 cut(s) 187, 258, 620, 863, 1083
BspANI GGCC 9 cut(s) 130, 305, 371, 485, 494, 503, 554, 705, 736
BspFNI CGCG 3 cut(s) 257, 318, 1094
BspLI GGNNCC 3 cut(s) 428, 493, 1014
BspMAI CTGCAG 3 cut(s) 535, 667, 814
BspMI ACCTGC 1 cut(s) 656
BspOI GCTAGC 2 cut(s) 680, 690
BspPI GGATC 1 cut(s) 871
BspT107I GGYRCC 1 cut(s) 426
BsrDI GCAATG 3 cut(s) 214, 1016, 1157
BsrFI RCCGGY 1 cut(s) 550
BsrGI TGTACA 2 cut(s) 417, 1239
BssAI RCCGGY 1 cut(s) 550
BssECI CCNNGG 5 cut(s) 300, 495, 505, 1092, 1260
BssMI GATC 5 cut(s) 187, 258, 620, 863, 1083
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 3 cut(s) 495, 505, 1260
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 3 cut(s) 171, 426, 431
BstACI GRCGYC 1 cut(s) 270
BstAUI TGTACA 2 cut(s) 417, 1239
BstC8I GCNNGC 6 cut(s) 205, 333, 678, 688, 1067, 1257
BstDEI CTNAG 1 cut(s) 458
BstDSI CCRYGG 1 cut(s) 1092
BstF5I GGATG 2 cut(s) 528, 1270
BstFNI CGCG 3 cut(s) 257, 318, 1094
BstH2I RGCGCY 1 cut(s) 677
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 5 cut(s) 190, 261, 623, 866, 1086
BstMBI GATC 5 cut(s) 187, 258, 620, 863, 1083
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 7 cut(s) 491, 500, 533, 671, 767, 1027, 1100
BstNI CCWGG 1 cut(s) 302
BstSCI CCNGG 2 cut(s) 300, 544
BstSFI CTRYAG 3 cut(s) 531, 663, 810
BstUI CGCG 3 cut(s) 257, 318, 1094
BstX2I RGATCY 2 cut(s) 863, 1083
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 2 cut(s) 863, 1083
BsuI GTATCC 1 cut(s) 662
BsuRI GGCC 9 cut(s) 130, 305, 371, 485, 494, 503, 554, 705, 736
BtgI CCRYGG 1 cut(s) 1092
BtsCI GGATG 2 cut(s) 528, 1270
BtsIMutI CAGTG 1 cut(s) 1131
BveI ACCTGC 1 cut(s) 656
Cac8I GCNNGC 6 cut(s) 205, 333, 678, 688, 1067, 1257
CfoI GCGC 1 cut(s) 676
Cfr10I RCCGGY 1 cut(s) 550
Cfr13I GGNCC 4 cut(s) 370, 492, 502, 548
Cfr42I CCGCGG 1 cut(s) 1095
CpoI CGGWCCG 1 cut(s) 548
CseI GACGC 1 cut(s) 259
Csp6I GTAC 4 cut(s) 418, 427, 887, 1240
CspI CGGWCCG 1 cut(s) 548
CviAII CATG 2 cut(s) 346, 538
CviQI GTAC 4 cut(s) 418, 427, 887, 1240
DdeI CTNAG 1 cut(s) 458
DpnI GATC 5 cut(s) 189, 260, 622, 865, 1085
DpnII GATC 5 cut(s) 187, 258, 620, 863, 1083
EaeI YGGCCR 5 cut(s) 303, 483, 552, 703, 734
EciI GGCGGA 1 cut(s) 137
Eco130I CCWWGG 3 cut(s) 495, 505, 1260
Eco147I AGGCCT 1 cut(s) 130
Eco24I GRGCYC 2 cut(s) 1015, 1193
Eco47I GGWCC 1 cut(s) 548
Eco47III AGCGCT 1 cut(s) 675
EcoO109I RGGNCCY 2 cut(s) 492, 502
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 3 cut(s) 495, 505, 1260
EcoT38I GRGCYC 2 cut(s) 1015, 1193
ErhI CCWWGG 3 cut(s) 495, 505, 1260
FaeI CATG 2 cut(s) 349, 541
FaqI GGGAC 3 cut(s) 32, 94, 326
FatI CATG 2 cut(s) 345, 537
FauI CCCGC 4 cut(s) 71, 205, 212, 683
FokI GGATG 2 cut(s) 535, 1277
FriOI GRGCYC 2 cut(s) 1015, 1193
FspBI CTAG 2 cut(s) 677, 687
GlaI GCGC 1 cut(s) 675
GsaI CCCAGC 1 cut(s) 1255
GsuI CTGGAG 1 cut(s) 738
HaeII RGCGCY 1 cut(s) 677
HaeIII GGCC 9 cut(s) 130, 305, 371, 485, 494, 503, 554, 705, 736
HapII CCGG 2 cut(s) 546, 551
HgaI GACGC 1 cut(s) 259
HhaI GCGC 1 cut(s) 676
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 2 cut(s) 349, 541
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HincII GTYRAC 2 cut(s) 291, 853
HindII GTYRAC 2 cut(s) 291, 853
HindIII AAGCTT 1 cut(s) 1063
HinfI GANTC 5 cut(s) 510, 568, 630, 698, 725
HpaII CCGG 2 cut(s) 546, 551
HphI GGTGA 2 cut(s) 56, 409
Hpy166II GTNNAC 2 cut(s) 291, 853
Hpy188I TCNGA 7 cut(s) 105, 187, 468, 573, 639, 863, 978
Hpy188III TCNNGA 3 cut(s) 717, 867, 1135
Hpy8I GTNNAC 2 cut(s) 291, 853
Hpy99I CGWCG 2 cut(s) 275, 311
HpyAV CCTTC 6 cut(s) 63, 97, 102, 147, 370, 1163
HpyCH4III ACNGT 3 cut(s) 171, 426, 431
HpyCH4IV ACGT 2 cut(s) 20, 575
HpyCH4V TGCA 8 cut(s) 533, 665, 776, 812, 947, 995, 1181, 1214
HpyF10VI GCNNNNNNNGC 7 cut(s) 491, 500, 533, 671, 767, 1027, 1100
HpyF3I CTNAG 1 cut(s) 458
HpySE526I ACGT 2 cut(s) 20, 575
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 2 cut(s) 349, 541
HspAI GCGC 1 cut(s) 674
KpnI GGTACC 1 cut(s) 430
KspI CCGCGG 1 cut(s) 1095
Kzo9I GATC 5 cut(s) 187, 258, 620, 863, 1083
LmnI GCTCC 4 cut(s) 222, 287, 986, 1018
LweI GCATC 4 cut(s) 699, 785, 796, 1004
MaeI CTAG 2 cut(s) 677, 687
MaeII ACGT 2 cut(s) 20, 575
MaeIII GTNAC 1 cut(s) 442
MalI GATC 5 cut(s) 189, 260, 622, 865, 1085
MboI GATC 5 cut(s) 187, 258, 620, 863, 1083
MboII GAAGA 4 cut(s) 361, 594, 630, 744
MflI RGATCY 2 cut(s) 863, 1083
MhlI GDGCHC 2 cut(s) 1015, 1193
MlsI TGGCCA 3 cut(s) 485, 705, 736
MluCI AATT 6 cut(s) 374, 470, 791, 1215, 1275, 1279
MluNI TGGCCA 3 cut(s) 485, 705, 736
MlyI GAGTC 3 cut(s) 519, 577, 734
MmeI TCCRAC 1 cut(s) 128
Mox20I TGGCCA 3 cut(s) 485, 705, 736
MscI TGGCCA 3 cut(s) 485, 705, 736
MseI TTAA 2 cut(s) 821, 1278
MslI CAYNNNNRTG 1 cut(s) 1115
Msp20I TGGCCA 3 cut(s) 485, 705, 736
MspA1I CMGCKG 2 cut(s) 1027, 1094
MspI CCGG 2 cut(s) 546, 551
MspR9I CCNGG 2 cut(s) 302, 546
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 3 cut(s) 257, 318, 1094
MwoI GCNNNNNNNGC 7 cut(s) 491, 500, 533, 671, 767, 1027, 1100
NciI CCSGG 1 cut(s) 546
NdeII GATC 5 cut(s) 187, 258, 620, 863, 1083
NheI GCTAGC 2 cut(s) 676, 686
NlaIII CATG 2 cut(s) 349, 541
NlaIV GGNNCC 3 cut(s) 428, 493, 1014
NmuCI GTSAC 1 cut(s) 442
PaqCI CACCTGC 1 cut(s) 656
PceI AGGCCT 1 cut(s) 130
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PfeI GAWTC 2 cut(s) 630, 698
PfoI TCCNGGA 1 cut(s) 544
Ple19I CGATCG 1 cut(s) 190
PleI GAGTC 3 cut(s) 518, 576, 733
PpsI GAGTC 3 cut(s) 518, 576, 733
PshBI ATTAAT 1 cut(s) 1278
Psp6I CCWGG 1 cut(s) 300
PspFI CCCAGC 1 cut(s) 1251
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 3 cut(s) 428, 493, 1014
PspPI GGNCC 4 cut(s) 370, 492, 502, 548
PstI CTGCAG 3 cut(s) 535, 667, 814
PsuI RGATCY 2 cut(s) 863, 1083
PvuI CGATCG 1 cut(s) 190
RsaI GTAC 4 cut(s) 419, 428, 888, 1241
RsaNI GTAC 4 cut(s) 418, 427, 887, 1240
RseI CAYNNNNRTG 1 cut(s) 1115
Rsr2I CGGWCCG 1 cut(s) 548
RsrII CGGWCCG 1 cut(s) 548
SacII CCGCGG 1 cut(s) 1095
SaqAI TTAA 2 cut(s) 821, 1278
Sau3AI GATC 5 cut(s) 187, 258, 620, 863, 1083
Sau96I GGNCC 4 cut(s) 370, 492, 502, 548
SchI GAGTC 3 cut(s) 519, 577, 734
ScrFI CCNGG 2 cut(s) 302, 546
SduI GDGCHC 2 cut(s) 1015, 1193
SfaNI GCATC 4 cut(s) 699, 785, 796, 1004
SfcI CTRYAG 3 cut(s) 531, 663, 810
SfiI GGCCNNNNNGGCC 2 cut(s) 491, 500
Sfr303I CCGCGG 1 cut(s) 1095
SgrBI CCGCGG 1 cut(s) 1095
SinI GGWCC 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 1115
Sse9I AATT 6 cut(s) 374, 470, 791, 1215, 1275, 1279
SseBI AGGCCT 1 cut(s) 130
SspI AATATT 1 cut(s) 414
SspMI CTAG 2 cut(s) 677, 687
StuI AGGCCT 1 cut(s) 130
StyD4I CCNGG 2 cut(s) 300, 544
StyI CCWWGG 3 cut(s) 495, 505, 1260
TaaI ACNGT 3 cut(s) 171, 426, 431
TaiI ACGT 2 cut(s) 23, 578
TaqI TCGA 1 cut(s) 1136
TasI AATT 6 cut(s) 374, 470, 791, 1215, 1275, 1279
TatI WGTACW 3 cut(s) 417, 886, 1239
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 2 cut(s) 630, 698
Tru1I TTAA 2 cut(s) 821, 1278
Tru9I TTAA 2 cut(s) 821, 1278
TscAI CASTG 1 cut(s) 1131
TseFI GTSAC 1 cut(s) 442
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 2 cut(s) 362, 367
TspGWI ACGGA 2 cut(s) 34, 274
TspRI CASTG 1 cut(s) 1131
VpaK11BI GGWCC 1 cut(s) 548
VspI ATTAAT 1 cut(s) 1278
XapI RAATTY 1 cut(s) 791
XcmI CCANNNNNNNNNTGG 2 cut(s) 493, 713
XspI CTAG 2 cut(s) 677, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.