MD08G1202800.v1.1

CRT-like, chloroquine-resistance transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003123
Physical Location & Seq
Reverse (-)
26227473 .. 26229799
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1202800.v1.1.491

Sequence Viewer

Length: 885 bp
ATGTCCACCACGTCGCCGTTTCACCACTTCTCCGCCCCTTTTCACGCCCGGCACTGCACCGTCAAAAATGGCGCGATCCCCTTCCCTTCTCTCTCCTCCTCCGCAATGCCGCTCCTTCAAAGCCCTCGACTCTCTCTTCTCCGCCTCCCTCAAAACGCACCGCTTCACCACACCTCTGAACGCTCCTCCTGCAGACCCTCACCGACAACTCGCGATGTAAAGGCCCGAGCATCGCTCCCAGAGCCGCCCTCCTCCACCTCCTCCACCTCCACCTTTACAAAACGCGTCGTTTTGAGCTCCGCATTGACCATAGCTCTGGCCGTCGCCAACCGCGTGTTCTACAAGCTGGCCCTCGTCCCCATGAAAAACCACCCTTTCTTCTTGGCCCAATTCACAACCTTCGGCTACGTGGTGATTTATTTTTCGATATTGGTCGTACGGTACCGTTCTGGGGTTGTTACTGACGAAATGATAGGTCTTCCCAAGTCACGGTTTGCCGCCATTGGTGCCCTAGAAGCCCTTGGAGTTGCTTCTGGAATGGCTGCTGCAGCCATGCTTCCCGGACCGGCCATACCCATATTGAGTCAGACGTTTTTGGTGTGGCAGCTGAGTTTTTCTGCCCTTCTCTTGGGGAGAACATACACATTTAATCAGATTGTTGGCTGCATACTCGTAGCTACAGGTGTAGCAGCGGCTGTTTCAAGGTACCTTGATCCATTCTTCCACAGATTAAAGAGCTGCTACTGTGTTTGGTTATCTTTAATATTGTCCATTTCTTCTGTCAGTAAAGCTGAAGGATCTGTGATACAGAACTTTTCAAGGTCATTACTAGTAGTCACTTGGATGTGGTGTACTTTAAACTCATGCCATGTCTACAAATGTTAA

Protein Analysis

295

Amino Acids

32.05

Weight (kDa)

10.01

Isoelectric Point (pI)

54.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 97 - 270 2.8e-22 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 441, 705
AccB1I GGYRCC 3 cut(s) 441, 506, 705
AccBSI CCGCTC 1 cut(s) 112
AccI GTMKAC 1 cut(s) 873
AccII CGCG 4 cut(s) 74, 213, 285, 333
AclWI GGATC 3 cut(s) 70, 707, 805
AcoI YGGCCR 2 cut(s) 318, 567
AcuI CTGAAG 1 cut(s) 813
AfaI GTAC 4 cut(s) 438, 443, 707, 853
AfiI CCNNNNNNNGG 3 cut(s) 451, 489, 628
AflIII ACRYGT 1 cut(s) 283
AgsI TTSAA 3 cut(s) 119, 702, 819
AhlI ACTAGT 1 cut(s) 829
AjiI CACGTC 1 cut(s) 12
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 7 cut(s) 297, 314, 346, 607, 677, 738, 791
AluI AGCT 7 cut(s) 297, 314, 346, 607, 677, 738, 791
Alw21I GWGCWC 1 cut(s) 299
AlwI GGATC 3 cut(s) 70, 707, 805
AlwNI CAGNNNCTG 1 cut(s) 695
Ama87I CYCGRG 1 cut(s) 225
AoxI GGCC 5 cut(s) 222, 318, 348, 384, 567
ApeKI GCWGC 7 cut(s) 542, 545, 548, 604, 663, 689, 738
Asp718I GGTACC 2 cut(s) 441, 705
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 4 cut(s) 223, 349, 385, 563
AsuC2I CCSGG 2 cut(s) 49, 561
AsuHPI GGTGA 4 cut(s) 14, 158, 192, 424
AvaI CYCGRG 1 cut(s) 225
AvaII GGWCC 1 cut(s) 563
BaeGI GKGCMC 1 cut(s) 511
BanI GGYRCC 3 cut(s) 441, 506, 705
BanII GRGCYC 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 470
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 7 cut(s) 529, 532, 560, 616, 650, 701, 725
BceAI ACGGC 1 cut(s) 305
BcnI CCSGG 2 cut(s) 49, 561
BcuI ACTAGT 1 cut(s) 829
BfaI CTAG 2 cut(s) 512, 830
BfmI CTRYAG 3 cut(s) 190, 546, 678
Bme1390I CCNGG 2 cut(s) 49, 561
Bme18I GGWCC 1 cut(s) 563
BmeT110I CYCGRG 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 12
BmgT120I GGNCC 4 cut(s) 223, 349, 385, 563
BmiI GGNNCC 3 cut(s) 443, 508, 707
BmrFI CCNGG 2 cut(s) 49, 561
BmsI GCATC 1 cut(s) 239
BpiI GAAGAC 1 cut(s) 470
BplI GAGNNNNNCTC 4 cut(s) 219, 251, 233, 265
BpuMI CCSGG 2 cut(s) 49, 561
BsaAI YACGTR 1 cut(s) 409
BsaJI CCNNGG 1 cut(s) 520
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 3 cut(s) 451, 489, 628
Bse118I RCCGGY 1 cut(s) 565
Bse3DI GCAATG 1 cut(s) 111
BseDI CCNNGG 1 cut(s) 520
BseGI GGATG 1 cut(s) 849
BseLI CCNNNNNNNGG 3 cut(s) 451, 489, 628
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 599
BseRI GAGGAG 5 cut(s) 85, 88, 175, 241, 250
BseSI GKGCMC 1 cut(s) 511
BseXI GCAGC 7 cut(s) 529, 532, 560, 616, 650, 701, 725
BsgI GTGCAG 1 cut(s) 40
Bsh1236I CGCG 4 cut(s) 74, 213, 285, 333
BshFI GGCC 5 cut(s) 224, 320, 350, 386, 569
BshNI GGYRCC 3 cut(s) 441, 506, 705
BsiHKAI GWGCWC 1 cut(s) 299
BsiHKCI CYCGRG 1 cut(s) 225
BsiSI CCGG 3 cut(s) 49, 561, 566
BsiWI CGTACG 1 cut(s) 436
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 3 cut(s) 451, 489, 628
BsmFI GGGAC 1 cut(s) 341
BsnI GGCC 5 cut(s) 224, 320, 350, 386, 569
BsoBI CYCGRG 1 cut(s) 225
Bsp1286I GDGCHC 2 cut(s) 299, 511
Bsp143I GATC 3 cut(s) 75, 712, 797
Bsp68I TCGCGA 1 cut(s) 213
BspANI GGCC 5 cut(s) 224, 320, 350, 386, 569
BspCNI CTCAG 1 cut(s) 600
BspFNI CGCG 4 cut(s) 74, 213, 285, 333
BspLI GGNNCC 3 cut(s) 443, 508, 707
BspMAI CTGCAG 2 cut(s) 194, 550
BspPI GGATC 3 cut(s) 70, 707, 805
BspT107I GGYRCC 3 cut(s) 441, 506, 705
BsrBI CCGCTC 1 cut(s) 112
BsrDI GCAATG 1 cut(s) 111
BsrFI RCCGGY 1 cut(s) 565
BssAI RCCGGY 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 520
BssMI GATC 3 cut(s) 75, 712, 797
BssT1I CCWWGG 1 cut(s) 520
Bst4CI ACNGT 5 cut(s) 61, 441, 446, 492, 746
Bst6I CTCTTC 1 cut(s) 141
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 1 cut(s) 608
BstF5I GGATG 1 cut(s) 849
BstFNI CGCG 4 cut(s) 74, 213, 285, 333
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 3 cut(s) 78, 715, 800
BstMBI GATC 3 cut(s) 75, 712, 797
BstMWI GCNNNNNNNGC 5 cut(s) 189, 241, 506, 515, 548
BstSCI CCNGG 2 cut(s) 47, 559
BstSFI CTRYAG 3 cut(s) 190, 546, 678
BstSLI GKGCMC 1 cut(s) 511
BstUI CGCG 4 cut(s) 74, 213, 285, 333
BstV1I GCAGC 7 cut(s) 529, 532, 560, 616, 650, 701, 725
BstV2I GAAGAC 1 cut(s) 470
BstX2I RGATCY 1 cut(s) 797
BstXI CCANNNNNNTGG 1 cut(s) 316
BstYI RGATCY 1 cut(s) 797
BsuRI GGCC 5 cut(s) 224, 320, 350, 386, 569
BtgZI GCGATG 2 cut(s) 216, 228
BtrI CACGTC 1 cut(s) 12
BtsCI GGATG 1 cut(s) 849
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
BtuMI TCGCGA 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 348
CaiI CAGNNNCTG 1 cut(s) 695
CfoI GCGC 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 565
Cfr13I GGNCC 4 cut(s) 223, 349, 385, 563
CpoI CGGWCCG 1 cut(s) 563
CseI GACGC 1 cut(s) 274
Csp6I GTAC 4 cut(s) 437, 442, 706, 852
CspI CGGWCCG 1 cut(s) 563
CviAII CATG 4 cut(s) 361, 553, 864, 869
CviQI GTAC 4 cut(s) 437, 442, 706, 852
DdeI CTNAG 1 cut(s) 608
DpnI GATC 3 cut(s) 77, 714, 799
DpnII GATC 3 cut(s) 75, 712, 797
DraI TTTAAA 1 cut(s) 858
EaeI YGGCCR 2 cut(s) 318, 567
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
EciI GGCGGA 2 cut(s) 22, 131
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 563
Eco53kI GAGCTC 1 cut(s) 297
Eco57I CTGAAG 1 cut(s) 813
Eco88I CYCGRG 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 297
EcoT14I CCWWGG 1 cut(s) 520
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 520
FaeI CATG 4 cut(s) 364, 556, 867, 872
FaiI YATR 9 cut(s) 311, 362, 554, 572, 578, 640, 668, 865, 870
FaqI GGGAC 1 cut(s) 341
FatI CATG 4 cut(s) 360, 552, 863, 868
FblI GTMKAC 1 cut(s) 873
FokI GGATG 1 cut(s) 856
FriOI GRGCYC 1 cut(s) 299
FspBI CTAG 2 cut(s) 512, 830
GlaI GCGC 1 cut(s) 73
HaeIII GGCC 5 cut(s) 224, 320, 350, 386, 569
HapII CCGG 3 cut(s) 49, 561, 566
HgaI GACGC 1 cut(s) 274
HhaI GCGC 1 cut(s) 74
Hin1II CATG 4 cut(s) 364, 556, 867, 872
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 2 cut(s) 129, 583
HpaII CCGG 3 cut(s) 49, 561, 566
HphI GGTGA 4 cut(s) 14, 158, 192, 424
Hpy166II GTNNAC 3 cut(s) 6, 852, 874
Hpy188I TCNGA 3 cut(s) 178, 588, 654
Hpy188III TCNNGA 2 cut(s) 212, 534
Hpy8I GTNNAC 3 cut(s) 6, 852, 874
Hpy99I CGWCG 3 cut(s) 16, 290, 326
HpyAV CCTTC 6 cut(s) 91, 96, 125, 409, 632, 788
HpyCH4III ACNGT 5 cut(s) 61, 441, 446, 492, 746
HpyCH4IV ACGT 3 cut(s) 11, 408, 590
HpyCH4V TGCA 4 cut(s) 57, 192, 548, 666
HpyF10VI GCNNNNNNNGC 5 cut(s) 189, 241, 506, 515, 548
HpyF3I CTNAG 1 cut(s) 608
HpySE526I ACGT 3 cut(s) 11, 408, 590
Hsp92II CATG 4 cut(s) 364, 556, 867, 872
HspAI GCGC 1 cut(s) 72
KpnI GGTACC 2 cut(s) 445, 709
Kzo9I GATC 3 cut(s) 75, 712, 797
LmnI GCTCC 4 cut(s) 117, 188, 240, 302
Lsp1109I GCAGC 7 cut(s) 529, 532, 560, 616, 650, 701, 725
LweI GCATC 1 cut(s) 239
MaeI CTAG 2 cut(s) 512, 830
MaeII ACGT 3 cut(s) 11, 408, 590
MaeIII GTNAC 3 cut(s) 457, 486, 835
MalI GATC 3 cut(s) 77, 714, 799
MbiI CCGCTC 1 cut(s) 112
MboI GATC 3 cut(s) 75, 712, 797
MboII GAAGA 5 cut(s) 128, 370, 470, 712, 768
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 2 cut(s) 299, 511
MluCI AATT 1 cut(s) 389
MluI ACGCGT 1 cut(s) 283
MlyI GAGTC 2 cut(s) 123, 592
MseI TTAA 5 cut(s) 648, 731, 761, 857, 883
MslI CAYNNNNRTG 1 cut(s) 842
MspA1I CMGCKG 2 cut(s) 607, 692
MspI CCGG 3 cut(s) 49, 561, 566
MspR9I CCNGG 2 cut(s) 49, 561
MvnI CGCG 4 cut(s) 74, 213, 285, 333
MwoI GCNNNNNNNGC 5 cut(s) 189, 241, 506, 515, 548
NciI CCSGG 2 cut(s) 49, 561
NdeII GATC 3 cut(s) 75, 712, 797
NlaIII CATG 4 cut(s) 364, 556, 867, 872
NlaIV GGNNCC 3 cut(s) 443, 508, 707
NmuCI GTSAC 2 cut(s) 486, 835
NruI TCGCGA 1 cut(s) 213
PcsI WCGNNNNNNNCGW 1 cut(s) 330
Pfl23II CGTACG 1 cut(s) 436
PfoI TCCNGGA 1 cut(s) 559
PleI GAGTC 2 cut(s) 123, 591
PpsI GAGTC 2 cut(s) 123, 591
Ppu21I YACGTR 1 cut(s) 409
Psp124BI GAGCTC 1 cut(s) 299
PspLI CGTACG 1 cut(s) 436
PspN4I GGNNCC 3 cut(s) 443, 508, 707
PspPI GGNCC 4 cut(s) 223, 349, 385, 563
PstI CTGCAG 2 cut(s) 194, 550
PstNI CAGNNNCTG 1 cut(s) 695
PsuI RGATCY 1 cut(s) 797
PvuII CAGCTG 1 cut(s) 607
RruI TCGCGA 1 cut(s) 213
RsaI GTAC 4 cut(s) 438, 443, 707, 853
RsaNI GTAC 4 cut(s) 437, 442, 706, 852
RseI CAYNNNNRTG 1 cut(s) 842
Rsr2I CGGWCCG 1 cut(s) 563
RsrII CGGWCCG 1 cut(s) 563
SacI GAGCTC 1 cut(s) 299
SaqAI TTAA 5 cut(s) 648, 731, 761, 857, 883
Sau3AI GATC 3 cut(s) 75, 712, 797
Sau96I GGNCC 4 cut(s) 223, 349, 385, 563
SchI GAGTC 2 cut(s) 123, 592
ScrFI CCNGG 2 cut(s) 49, 561
SduI GDGCHC 2 cut(s) 299, 511
SfaNI GCATC 1 cut(s) 239
SfcI CTRYAG 3 cut(s) 190, 546, 678
SinI GGWCC 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 842
SpeI ACTAGT 1 cut(s) 829
Sse9I AATT 1 cut(s) 389
SspI AATATT 1 cut(s) 765
SspMI CTAG 2 cut(s) 512, 830
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 47, 559
StyI CCWWGG 1 cut(s) 520
TaaI ACNGT 5 cut(s) 61, 441, 446, 492, 746
TaiI ACGT 3 cut(s) 14, 411, 593
TaqI TCGA 2 cut(s) 127, 425
TasI AATT 1 cut(s) 389
TatI WGTACW 1 cut(s) 851
TauI GCSGC 4 cut(s) 112, 247, 500, 695
Tru1I TTAA 5 cut(s) 648, 731, 761, 857, 883
Tru9I TTAA 5 cut(s) 648, 731, 761, 857, 883
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 2 cut(s) 486, 835
TseI GCWGC 7 cut(s) 542, 545, 548, 604, 663, 689, 738
Tsp45I GTSAC 2 cut(s) 486, 835
TspDTI ATGAA 1 cut(s) 377
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 873
XspI CTAG 2 cut(s) 512, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.