Rroxscaffold_3G00226420

CRT-like, chloroquine-resistance transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003123
Physical Location & Seq
Reverse (-)
9937236 .. 9943230
5995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00226420.1

Sequence Viewer

Length: 1359 bp
ATGGGTTCCTCAACGGCGTCGTTTCACAAGTTCCTCTCCCTCCCTTCCCACACCCACGCCAAAAATGGCGCAACTCGCAACCCTTTATTCTCACACTCAAGGCCGCTTCTTCTGCTTCATCCGAGGTGGCGTCATCATCATCATCATCATCATCGTCATCGACTTTCTCTCGCTACTCTAAACGCACCGTTTTGCCTCATTTCGGATCGCGGACCAAGGTGCATAGCCAGGCGCAATGTCAGGCTGCACGCTTCGGTCGCCAACCCGGCTTCCTCAGAAAATCCGTCGACTTCAAAACGCGTCGTTTTGAGCTCCCTGTTGACCATTGCTCTCGCCGTAGGGAACAAGGTCTTCTACAAGCTCGCACTCGTTCCCATGAAGAACCACCCTTTCTTTTTCGCCCAATTCACTACCTTCGGTTATGTGATTATATACTTTTCAATATTGTACATACGGTACCGTTCTGGGAGGGTGACGGATGAAATGCTAGCCCTTCCCAAAGCTCCATTTGTGGCCATTGGTGCCCTGGATGCTCTCGGAGTCGCTGCTGGGATGGCTGCTGTAGCCATGCTTCCCGGACCGGCCATACCCTTATTGAGTCAGACAATGTTGGTGTGGCAGCTGGGTTTTTCTTCTCTTATCTTGGGAAAAAGATACAGATGCAATCAGATTGTTGGCTGCATCCTTGTAGCTGTAGGTGTAGCGCTAGCTGTGGCAAGCGGGTCTGATTCTGGCCAGATGCTATCTGGAGTTGAGCCTGTGTGGCCTCTACTAATGATTGCTTCAAGTGCTTTTCAAGCTGGTGCATTTATAATCAAGGAATTTATCTTTGTTGATGCTGCTGTCCACCTTAAGGAAAAATCACTTGACATATTGGTTGTCAACTCTTTTGGATCTGCATATCAGGCTCTTTTTGTACTTCTATTTCTACCTTTCTTATCCAATATGAGAGGCATACCATTTTCCCAACTTCCTACATATCTAAAAGACGGTGCTGGTTGCTTTCTGAATATTGGAGCTGGTGCATCAGGCAAGCTGTTGTATTTGTTGCAATGGAGCTCCATTGCTCCCCCTGCTTTACGTAGCTGCCAATCTGTTGTTCAATATATCCTTGATGAATGTAGTAAAGATTTCTTCGGCGGTCGTTTCTTCTCTCGTTGTGATGTTGTCAGGTTCTCTCTGATCTCTCTTGCTTGGATTTTGCCAATCTCGATTTATGTTCTTTCCCTTCCATTGCCATATCTCCAAGAGGGCGCAACTTTGAGCCCCTTTTTCCTCTTTGGTAGTGCGATTCTTGTCTTGGGTCTTATTCTGTACAGCATACCCCAGCCTGCCAAGGATGGCTCTAAAATTAATTGA

Protein Analysis

452

Amino Acids

49.34

Weight (kDa)

9.84

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 102 - 339 8.4e-36 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 812
Acc65I GGTACC 1 cut(s) 456
AccB1I GGYRCC 2 cut(s) 456, 521
AccI GTMKAC 1 cut(s) 287
AccII CGCG 2 cut(s) 210, 300
AciI CCGC 4 cut(s) 104, 210, 720, 1140
AclWI GGATC 2 cut(s) 213, 901
AcoI YGGCCR 3 cut(s) 513, 582, 733
AcsI RAATTY 1 cut(s) 821
AcyI GRCGYC 2 cut(s) 17, 130
AfaI GTAC 4 cut(s) 449, 458, 918, 1316
AfeI AGCGCT 1 cut(s) 705
AfiI CCNNNNNNNGG 2 cut(s) 202, 853
AflII CTTAAG 1 cut(s) 851
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 5 cut(s) 294, 441, 786, 797, 1103
AjnI CCWGG 2 cut(s) 227, 525
AjuI GAANNNNNNNTTGG 2 cut(s) 1083, 1115
Alw21I GWGCWC 2 cut(s) 314, 1061
AlwI GGATC 2 cut(s) 213, 901
Aor51HI AGCGCT 1 cut(s) 705
AoxI GGCC 5 cut(s) 101, 513, 582, 733, 764
ApeKI GCWGC 7 cut(s) 244, 545, 557, 619, 678, 839, 1086
ApoI RAATTY 1 cut(s) 821
AseI ATTAAT 1 cut(s) 1353
Asp718I GGTACC 1 cut(s) 456
AspLEI GCGC 4 cut(s) 71, 234, 706, 1256
AspS9I GGNCC 2 cut(s) 212, 578
AsuC2I CCSGG 2 cut(s) 266, 576
AsuHPI GGTGA 1 cut(s) 484
AsuNHI GCTAGC 2 cut(s) 487, 706
AvaII GGWCC 2 cut(s) 212, 578
BaeGI GKGCMC 1 cut(s) 526
BalI TGGCCA 2 cut(s) 515, 735
BanI GGYRCC 2 cut(s) 456, 521
BanII GRGCYC 3 cut(s) 314, 1061, 1268
BarI GAAGNNNNNNTAC 2 cut(s) 555, 587
BbsI GAAGAC 1 cut(s) 343
Bbv12I GWGCWC 2 cut(s) 314, 1061
BbvI GCAGC 7 cut(s) 231, 532, 544, 631, 665, 826, 1073
BccI CCATC 2 cut(s) 547, 1334
BceAI ACGGC 2 cut(s) 30, 320
BciT130I CCWGG 2 cut(s) 229, 527
BcnI CCSGG 2 cut(s) 266, 576
BfaI CTAG 2 cut(s) 488, 707
BfmI CTRYAG 2 cut(s) 561, 693
BfoI RGCGCY 1 cut(s) 707
BfrI CTTAAG 1 cut(s) 851
BglI GCCNNNNNGGC 2 cut(s) 266, 763
BisI GCNGC 8 cut(s) 104, 245, 546, 558, 620, 679, 840, 1087
BlsI GCNGC 8 cut(s) 105, 246, 547, 559, 621, 680, 841, 1088
Bme1390I CCNGG 4 cut(s) 229, 266, 527, 576
Bme18I GGWCC 2 cut(s) 212, 578
BmgT120I GGNCC 2 cut(s) 212, 578
BmiI GGNNCC 3 cut(s) 7, 458, 523
BmrFI CCNGG 4 cut(s) 229, 266, 527, 576
BmsI GCATC 6 cut(s) 520, 650, 690, 729, 826, 1034
BmtI GCTAGC 2 cut(s) 491, 710
BpiI GAAGAC 1 cut(s) 343
BpmI CTGGAG 1 cut(s) 768
BpuEI CTTGAG 1 cut(s) 82
BpuMI CCSGG 2 cut(s) 266, 576
BsaAI YACGTR 1 cut(s) 1082
BsaHI GRCGYC 2 cut(s) 17, 130
BsaJI CCNNGG 4 cut(s) 122, 215, 525, 1335
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 853
Bse118I RCCGGY 1 cut(s) 580
Bse3DI GCAATG 5 cut(s) 241, 324, 1058, 1062, 1232
BseBI CCWGG 2 cut(s) 229, 527
BseDI CCNNGG 4 cut(s) 122, 215, 525, 1335
BseGI GGATG 6 cut(s) 118, 484, 535, 558, 681, 1345
BseLI CCNNNNNNNGG 2 cut(s) 202, 853
BseMI GCAATG 5 cut(s) 241, 324, 1058, 1062, 1232
BseMII CTCAG 1 cut(s) 288
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 7 cut(s) 231, 532, 544, 631, 665, 826, 1073
BseYI CCCAGC 3 cut(s) 548, 622, 1326
BsgI GTGCAG 1 cut(s) 230
Bsh1236I CGCG 2 cut(s) 210, 300
Bsh1285I CGRYCG 2 cut(s) 258, 1144
BshFI GGCC 5 cut(s) 103, 515, 584, 735, 766
BshNI GGYRCC 2 cut(s) 456, 521
BsiEI CGRYCG 2 cut(s) 258, 1144
BsiHKAI GWGCWC 2 cut(s) 314, 1061
BsiSI CCGG 3 cut(s) 266, 576, 581
BslI CCNNNNNNNGG 2 cut(s) 202, 853
BsnI GGCC 5 cut(s) 103, 515, 584, 735, 766
Bsp1286I GDGCHC 4 cut(s) 314, 526, 1061, 1268
Bsp1407I TGTACA 2 cut(s) 447, 1314
Bsp143I GATC 3 cut(s) 205, 893, 1182
BspACI CCGC 4 cut(s) 104, 210, 720, 1140
BspANI GGCC 5 cut(s) 103, 515, 584, 735, 766
BspCNI CTCAG 1 cut(s) 287
BspFNI CGCG 2 cut(s) 210, 300
BspLI GGNNCC 3 cut(s) 7, 458, 523
BspOI GCTAGC 2 cut(s) 491, 710
BspPI GGATC 2 cut(s) 213, 901
BspT107I GGYRCC 2 cut(s) 456, 521
BspTI CTTAAG 1 cut(s) 851
BsrDI GCAATG 5 cut(s) 241, 324, 1058, 1062, 1232
BsrFI RCCGGY 1 cut(s) 580
BsrGI TGTACA 2 cut(s) 447, 1314
BssAI RCCGGY 1 cut(s) 580
BssECI CCNNGG 4 cut(s) 122, 215, 525, 1335
BssMI GATC 3 cut(s) 205, 893, 1182
BssNI GRCGYC 2 cut(s) 17, 130
BssT1I CCWWGG 2 cut(s) 215, 1335
Bst2UI CCWGG 2 cut(s) 229, 527
Bst4CI ACNGT 4 cut(s) 189, 456, 461, 992
BstACI GRCGYC 2 cut(s) 17, 130
BstAFI CTTAAG 1 cut(s) 851
BstAUI TGTACA 2 cut(s) 447, 1314
BstBAI YACGTR 1 cut(s) 1082
BstC8I GCNNGC 7 cut(s) 249, 363, 489, 708, 718, 1034, 1332
BstDEI CTNAG 1 cut(s) 274
BstF5I GGATG 6 cut(s) 118, 484, 535, 558, 681, 1345
BstFNI CGCG 2 cut(s) 210, 300
BstH2I RGCGCY 1 cut(s) 707
BstHHI GCGC 4 cut(s) 71, 234, 706, 1256
BstKTI GATC 3 cut(s) 208, 896, 1185
BstMBI GATC 3 cut(s) 205, 893, 1182
BstMCI CGRYCG 2 cut(s) 258, 1144
BstNI CCWGG 2 cut(s) 229, 527
BstSCI CCNGG 4 cut(s) 227, 264, 525, 574
BstSFI CTRYAG 2 cut(s) 561, 693
BstSLI GKGCMC 1 cut(s) 526
BstSNI TACGTA 1 cut(s) 1082
BstUI CGCG 2 cut(s) 210, 300
BstV1I GCAGC 7 cut(s) 231, 532, 544, 631, 665, 826, 1073
BstV2I GAAGAC 1 cut(s) 343
BstX2I RGATCY 1 cut(s) 893
BstYI RGATCY 1 cut(s) 893
BsuRI GGCC 5 cut(s) 103, 515, 584, 735, 766
BtsCI GGATG 6 cut(s) 118, 484, 535, 558, 681, 1345
Cac8I GCNNGC 7 cut(s) 249, 363, 489, 708, 718, 1034, 1332
CfoI GCGC 4 cut(s) 71, 234, 706, 1256
Cfr10I RCCGGY 1 cut(s) 580
Cfr13I GGNCC 2 cut(s) 212, 578
CpoI CGGWCCG 1 cut(s) 578
CseI GACGC 3 cut(s) 6, 119, 289
Csp6I GTAC 4 cut(s) 448, 457, 917, 1315
CspI CGGWCCG 1 cut(s) 578
CviAII CATG 2 cut(s) 376, 568
CviQI GTAC 4 cut(s) 448, 457, 917, 1315
DdeI CTNAG 1 cut(s) 274
DpnI GATC 3 cut(s) 207, 895, 1184
DpnII GATC 3 cut(s) 205, 893, 1182
EaeI YGGCCR 3 cut(s) 513, 582, 733
Ecl136II GAGCTC 2 cut(s) 312, 1059
Eco105I TACGTA 1 cut(s) 1082
Eco130I CCWWGG 2 cut(s) 215, 1335
Eco24I GRGCYC 3 cut(s) 314, 1061, 1268
Eco47I GGWCC 2 cut(s) 212, 578
Eco47III AGCGCT 1 cut(s) 705
Eco53kI GAGCTC 2 cut(s) 312, 1059
EcoICRI GAGCTC 2 cut(s) 312, 1059
EcoRII CCWGG 2 cut(s) 227, 525
EcoT14I CCWWGG 2 cut(s) 215, 1335
EcoT38I GRGCYC 3 cut(s) 314, 1061, 1268
ErhI CCWWGG 2 cut(s) 215, 1335
FaeI CATG 2 cut(s) 379, 571
FatI CATG 2 cut(s) 375, 567
FauI CCCGC 1 cut(s) 713
FblI GTMKAC 1 cut(s) 287
Fnu4HI GCNGC 8 cut(s) 104, 245, 546, 558, 620, 679, 840, 1087
FokI GGATG 6 cut(s) 105, 491, 542, 565, 668, 1352
FriOI GRGCYC 3 cut(s) 314, 1061, 1268
Fsp4HI GCNGC 8 cut(s) 104, 245, 546, 558, 620, 679, 840, 1087
FspBI CTAG 2 cut(s) 488, 707
GlaI GCGC 4 cut(s) 70, 233, 705, 1255
GluI GCNGC 8 cut(s) 104, 245, 546, 558, 620, 679, 840, 1087
GsaI CCCAGC 3 cut(s) 552, 626, 1330
GsuI CTGGAG 1 cut(s) 768
HaeII RGCGCY 1 cut(s) 707
HaeIII GGCC 5 cut(s) 103, 515, 584, 735, 766
HapII CCGG 3 cut(s) 266, 576, 581
HgaI GACGC 3 cut(s) 6, 119, 289
HhaI GCGC 4 cut(s) 71, 234, 706, 1256
Hin1I GRCGYC 2 cut(s) 17, 130
Hin1II CATG 2 cut(s) 379, 571
Hin6I GCGC 4 cut(s) 69, 232, 704, 1254
HinP1I GCGC 4 cut(s) 69, 232, 704, 1254
HincII GTYRAC 3 cut(s) 288, 321, 883
HindII GTYRAC 3 cut(s) 288, 321, 883
HinfI GANTC 4 cut(s) 540, 598, 728, 1291
HpaII CCGG 3 cut(s) 266, 576, 581
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 4 cut(s) 288, 321, 847, 883
Hpy188I TCNGA 9 cut(s) 123, 205, 277, 539, 603, 669, 727, 1008, 1182
Hpy188III TCNNGA 2 cut(s) 747, 1210
Hpy8I GTNNAC 4 cut(s) 288, 321, 847, 883
Hpy99I CGWCG 3 cut(s) 22, 289, 305
HpyAV CCTTC 4 cut(s) 54, 424, 503, 1238
HpyCH4III ACNGT 4 cut(s) 189, 456, 461, 992
HpyCH4IV ACGT 1 cut(s) 1081
HpyCH4V TGCA 8 cut(s) 222, 247, 663, 681, 806, 899, 1025, 1051
HpyF3I CTNAG 1 cut(s) 274
HpySE526I ACGT 1 cut(s) 1081
Hsp92I GRCGYC 2 cut(s) 17, 130
Hsp92II CATG 2 cut(s) 379, 571
HspAI GCGC 4 cut(s) 69, 232, 704, 1254
KpnI GGTACC 1 cut(s) 460
Kzo9I GATC 3 cut(s) 205, 893, 1182
LmnI GCTCC 6 cut(s) 317, 508, 1016, 1056, 1064, 1072
Lsp1109I GCAGC 7 cut(s) 231, 532, 544, 631, 665, 826, 1073
LweI GCATC 6 cut(s) 520, 650, 690, 729, 826, 1034
MaeI CTAG 2 cut(s) 488, 707
MaeII ACGT 1 cut(s) 1081
MaeIII GTNAC 1 cut(s) 472
MalI GATC 3 cut(s) 207, 895, 1184
MboI GATC 3 cut(s) 205, 893, 1182
MboII GAAGA 6 cut(s) 101, 343, 391, 624, 1126, 1141
MflI RGATCY 1 cut(s) 893
MhlI GDGCHC 4 cut(s) 314, 526, 1061, 1268
MlsI TGGCCA 2 cut(s) 515, 735
MluCI AATT 4 cut(s) 404, 821, 1350, 1354
MluI ACGCGT 1 cut(s) 298
MluNI TGGCCA 2 cut(s) 515, 735
MlyI GAGTC 2 cut(s) 549, 607
Mox20I TGGCCA 2 cut(s) 515, 735
MscI TGGCCA 2 cut(s) 515, 735
MseI TTAA 2 cut(s) 852, 1353
Msp20I TGGCCA 2 cut(s) 515, 735
MspA1I CMGCKG 1 cut(s) 622
MspCI CTTAAG 1 cut(s) 851
MspI CCGG 3 cut(s) 266, 576, 581
MspR9I CCNGG 4 cut(s) 229, 266, 527, 576
MvaI CCWGG 2 cut(s) 229, 527
MvnI CGCG 2 cut(s) 210, 300
NciI CCSGG 2 cut(s) 266, 576
NdeII GATC 3 cut(s) 205, 893, 1182
NheI GCTAGC 2 cut(s) 487, 706
NlaIII CATG 2 cut(s) 379, 571
NlaIV GGNNCC 3 cut(s) 7, 458, 523
NmuCI GTSAC 1 cut(s) 472
PfeI GAWTC 2 cut(s) 728, 1291
PfoI TCCNGGA 1 cut(s) 574
PkrI GCNGC 8 cut(s) 105, 246, 547, 559, 621, 680, 841, 1088
PleI GAGTC 2 cut(s) 548, 606
PpsI GAGTC 2 cut(s) 548, 606
Ppu21I YACGTR 1 cut(s) 1082
PshBI ATTAAT 1 cut(s) 1353
PsiI TTATAA 1 cut(s) 812
Psp124BI GAGCTC 2 cut(s) 314, 1061
Psp6I CCWGG 2 cut(s) 227, 525
PspFI CCCAGC 3 cut(s) 548, 622, 1326
PspGI CCWGG 2 cut(s) 227, 525
PspN4I GGNNCC 3 cut(s) 7, 458, 523
PspPI GGNCC 2 cut(s) 212, 578
PsuI RGATCY 1 cut(s) 893
PvuII CAGCTG 1 cut(s) 622
RsaI GTAC 4 cut(s) 449, 458, 918, 1316
RsaNI GTAC 4 cut(s) 448, 457, 917, 1315
Rsr2I CGGWCCG 1 cut(s) 578
RsrII CGGWCCG 1 cut(s) 578
SacI GAGCTC 2 cut(s) 314, 1061
SalI GTCGAC 1 cut(s) 286
SaqAI TTAA 2 cut(s) 852, 1353
SatI GCNGC 8 cut(s) 104, 245, 546, 558, 620, 679, 840, 1087
Sau3AI GATC 3 cut(s) 205, 893, 1182
Sau96I GGNCC 2 cut(s) 212, 578
SchI GAGTC 2 cut(s) 549, 607
ScrFI CCNGG 4 cut(s) 229, 266, 527, 576
SduI GDGCHC 4 cut(s) 314, 526, 1061, 1268
SfaNI GCATC 6 cut(s) 520, 650, 690, 729, 826, 1034
SfcI CTRYAG 2 cut(s) 561, 693
SinI GGWCC 2 cut(s) 212, 578
SmlI CTYRAG 2 cut(s) 97, 851
SmoI CTYRAG 2 cut(s) 97, 851
SnaBI TACGTA 1 cut(s) 1082
Sse9I AATT 4 cut(s) 404, 821, 1350, 1354
SsiI CCGC 4 cut(s) 104, 210, 720, 1140
SspI AATATT 2 cut(s) 444, 1012
SspMI CTAG 2 cut(s) 488, 707
SstI GAGCTC 2 cut(s) 314, 1061
StyD4I CCNGG 4 cut(s) 227, 264, 525, 574
StyI CCWWGG 2 cut(s) 215, 1335
TaaI ACNGT 4 cut(s) 189, 456, 461, 992
TaiI ACGT 1 cut(s) 1084
TaqI TCGA 3 cut(s) 160, 287, 1211
TaqII GACCGA 1 cut(s) 244
TasI AATT 4 cut(s) 404, 821, 1350, 1354
TatI WGTACW 3 cut(s) 447, 916, 1314
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 2 cut(s) 728, 1291
Tru1I TTAA 2 cut(s) 852, 1353
Tru9I TTAA 2 cut(s) 852, 1353
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 7 cut(s) 244, 545, 557, 619, 678, 839, 1086
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 4 cut(s) 107, 392, 495, 1131
TspGWI ACGGA 2 cut(s) 273, 491
Vha464I CTTAAG 1 cut(s) 851
VpaK11BI GGWCC 2 cut(s) 212, 578
VspI ATTAAT 1 cut(s) 1353
XapI RAATTY 1 cut(s) 821
XcmI CCANNNNNNNNNTGG 3 cut(s) 62, 523, 743
XmiI GTMKAC 1 cut(s) 287
XspI CTAG 2 cut(s) 488, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.