RchiOBHm_Chr7g0234951

CRT-like, chloroquine-resistance transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003123
Physical Location & Seq
Forward (+)
59646636 .. 59650170
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21056

Sequence Viewer

Length: 792 bp
ATGGGTTCCTCAACGGCGTCGTTTCACAACTTCCTCTCCCTCCCTTCCCACACCAAAAATGGCGCAACTCGCAACCCTCTATTCTCACACTCGAGGCCGCTTCTTCTGCTTCATCAGAGGTGGCGTCATCATCATCGGCTCTCTCTCGCTACTCTAAACGCACCGTTTTGCCTCACTTCGGATCGCCGACCCGCCAGGCGCAATGTCAGGCTGCACGCTTCGGTCGCCAACCCGGCTTCCTCCTCTAATCCGTCGACTTCAAAACGCGTCGTTTTGAGCTCCCTGTTGACCATTGCTCTCGCCGTCGGCAACAAGGTCTTCTACAAGCTCGCGCTCGTTCCCATGAAGAACCACCCTTTCTTTTTCGCCCAATTCACTACCTTCGGTTATGTGATTATATACTTTTCAATATTGTACATACGGTACCGTTCTGGGAGGGTGACGGATGAAATGCTAGCCCTTCCCAAAGCTCCATTTGTGGCCATTGGTGCCTTGGATGCTCTCGGAGTCGCTGCTGGGATGGCTGCCGTAGCCATGCTTCCCGGACCGGCCATACCCTTATTGAGTCAGACAATGTTGGTGTGGCAGCTGGGTTTTTCTTCTCTTATATTGGGAAAAAGATACAGATGCAATCAGATTGTTGGCTGCATCCTTGTAGCTGTAGGTGTAGCGCTAGCTGTGGCAAGCGGGTCTAATTCTGGCCAGATGCTATCTGGAGTTGAGCCTGTGTGGCCTTTACTAATGATTGCTTCAAGTGCTTTTCAAGCTGGTGCATTTATAATCAAGGTTTAA

Protein Analysis

263

Amino Acids

28.43

Weight (kDa)

11.14

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 91 - 252 3.5e-28 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 779
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 2 cut(s) 423, 488
AccI GTMKAC 1 cut(s) 254
AccII CGCG 2 cut(s) 267, 332
AciI CCGC 3 cut(s) 98, 192, 687
AclWI GGATC 1 cut(s) 189
AcoI YGGCCR 3 cut(s) 480, 549, 700
AcyI GRCGYC 2 cut(s) 17, 124
AfaI GTAC 2 cut(s) 416, 425
AfeI AGCGCT 1 cut(s) 672
AfiI CCNNNNNNNGG 1 cut(s) 178
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 4 cut(s) 261, 408, 753, 764
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 7 cut(s) 279, 328, 470, 589, 659, 677, 767
AluI AGCT 7 cut(s) 279, 328, 470, 589, 659, 677, 767
Alw21I GWGCWC 1 cut(s) 281
AlwI GGATC 1 cut(s) 189
Ama87I CYCGRG 1 cut(s) 91
Aor51HI AGCGCT 1 cut(s) 672
AoxI GGCC 5 cut(s) 95, 480, 549, 700, 731
ApeKI GCWGC 5 cut(s) 211, 512, 524, 586, 645
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 4 cut(s) 65, 201, 334, 673
AspS9I GGNCC 1 cut(s) 545
AsuC2I CCSGG 2 cut(s) 233, 543
AsuHPI GGTGA 1 cut(s) 451
AsuNHI GCTAGC 2 cut(s) 454, 673
AvaI CYCGRG 1 cut(s) 91
AvaII GGWCC 1 cut(s) 545
BalI TGGCCA 2 cut(s) 482, 702
BanI GGYRCC 2 cut(s) 423, 488
BanII GRGCYC 1 cut(s) 281
BarI GAAGNNNNNNTAC 2 cut(s) 522, 554
BbsI GAAGAC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 281
BbvI GCAGC 5 cut(s) 198, 499, 511, 598, 632
BccI CCATC 1 cut(s) 514
BceAI ACGGC 3 cut(s) 30, 287, 512
BciT130I CCWGG 1 cut(s) 196
BcnI CCSGG 2 cut(s) 233, 543
BfaI CTAG 2 cut(s) 455, 674
BfmI CTRYAG 1 cut(s) 660
BfoI RGCGCY 1 cut(s) 674
BglI GCCNNNNNGGC 2 cut(s) 233, 730
BisI GCNGC 6 cut(s) 98, 212, 513, 525, 587, 646
BlsI GCNGC 6 cut(s) 99, 213, 514, 526, 588, 647
Bme1390I CCNGG 3 cut(s) 196, 233, 543
Bme18I GGWCC 1 cut(s) 545
BmeT110I CYCGRG 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 545
BmiI GGNNCC 3 cut(s) 7, 425, 490
BmrFI CCNGG 3 cut(s) 196, 233, 543
BmsI GCATC 4 cut(s) 487, 617, 657, 696
BmtI GCTAGC 2 cut(s) 458, 677
BpiI GAAGAC 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 735
BpuMI CCSGG 2 cut(s) 233, 543
BsaHI GRCGYC 2 cut(s) 17, 124
BsaJI CCNNGG 1 cut(s) 492
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 547
Bse3DI GCAATG 2 cut(s) 208, 291
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 4 cut(s) 451, 502, 525, 648
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMI GCAATG 2 cut(s) 208, 291
BseRI GAGGAG 1 cut(s) 232
BseXI GCAGC 5 cut(s) 198, 499, 511, 598, 632
BseYI CCCAGC 2 cut(s) 515, 589
BsgI GTGCAG 1 cut(s) 197
Bsh1236I CGCG 2 cut(s) 267, 332
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 5 cut(s) 97, 482, 551, 702, 733
BshNI GGYRCC 2 cut(s) 423, 488
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 281
BsiHKCI CYCGRG 1 cut(s) 91
BsiSI CCGG 3 cut(s) 233, 543, 548
BslI CCNNNNNNNGG 1 cut(s) 178
BsnI GGCC 5 cut(s) 97, 482, 551, 702, 733
BsoBI CYCGRG 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 281
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 3 cut(s) 98, 192, 687
BspANI GGCC 5 cut(s) 97, 482, 551, 702, 733
BspFNI CGCG 2 cut(s) 267, 332
BspLI GGNNCC 3 cut(s) 7, 425, 490
BspOI GCTAGC 2 cut(s) 458, 677
BspPI GGATC 1 cut(s) 189
BspT107I GGYRCC 2 cut(s) 423, 488
BsrDI GCAATG 2 cut(s) 208, 291
BsrFI RCCGGY 1 cut(s) 547
BsrGI TGTACA 1 cut(s) 414
BssAI RCCGGY 1 cut(s) 547
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 1 cut(s) 181
BssNI GRCGYC 2 cut(s) 17, 124
BssT1I CCWWGG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 3 cut(s) 165, 423, 428
BstACI GRCGYC 2 cut(s) 17, 124
BstAUI TGTACA 1 cut(s) 414
BstC8I GCNNGC 5 cut(s) 216, 330, 456, 675, 685
BstF5I GGATG 4 cut(s) 451, 502, 525, 648
BstFNI CGCG 2 cut(s) 267, 332
BstH2I RGCGCY 1 cut(s) 674
BstHHI GCGC 4 cut(s) 65, 201, 334, 673
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstMCI CGRYCG 1 cut(s) 225
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 3 cut(s) 194, 231, 541
BstSFI CTRYAG 1 cut(s) 660
BstUI CGCG 2 cut(s) 267, 332
BstV1I GCAGC 5 cut(s) 198, 499, 511, 598, 632
BstV2I GAAGAC 1 cut(s) 310
BsuRI GGCC 5 cut(s) 97, 482, 551, 702, 733
BtsCI GGATG 4 cut(s) 451, 502, 525, 648
Cac8I GCNNGC 5 cut(s) 216, 330, 456, 675, 685
CfoI GCGC 4 cut(s) 65, 201, 334, 673
Cfr10I RCCGGY 1 cut(s) 547
Cfr13I GGNCC 1 cut(s) 545
CpoI CGGWCCG 1 cut(s) 545
CseI GACGC 3 cut(s) 6, 113, 256
Csp6I GTAC 2 cut(s) 415, 424
CspI CGGWCCG 1 cut(s) 545
CviAII CATG 2 cut(s) 343, 535
CviQI GTAC 2 cut(s) 415, 424
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
EaeI YGGCCR 3 cut(s) 480, 549, 700
Ecl136II GAGCTC 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 492
Eco24I GRGCYC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 545
Eco47III AGCGCT 1 cut(s) 672
Eco53kI GAGCTC 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 492
EcoT38I GRGCYC 1 cut(s) 281
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 2 cut(s) 346, 538
FaiI YATR 9 cut(s) 344, 390, 398, 400, 419, 536, 554, 608, 779
FatI CATG 2 cut(s) 342, 534
FauI CCCGC 2 cut(s) 199, 680
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 6 cut(s) 98, 212, 513, 525, 587, 646
FokI GGATG 4 cut(s) 458, 509, 532, 635
FriOI GRGCYC 1 cut(s) 281
Fsp4HI GCNGC 6 cut(s) 98, 212, 513, 525, 587, 646
FspBI CTAG 2 cut(s) 455, 674
GlaI GCGC 4 cut(s) 64, 200, 333, 672
GluI GCNGC 6 cut(s) 98, 212, 513, 525, 587, 646
GsaI CCCAGC 2 cut(s) 519, 593
GsuI CTGGAG 1 cut(s) 735
HaeII RGCGCY 1 cut(s) 674
HaeIII GGCC 5 cut(s) 97, 482, 551, 702, 733
HapII CCGG 3 cut(s) 233, 543, 548
HgaI GACGC 3 cut(s) 6, 113, 256
HhaI GCGC 4 cut(s) 65, 201, 334, 673
Hin1I GRCGYC 2 cut(s) 17, 124
Hin1II CATG 2 cut(s) 346, 538
Hin6I GCGC 4 cut(s) 63, 199, 332, 671
HinP1I GCGC 4 cut(s) 63, 199, 332, 671
HincII GTYRAC 2 cut(s) 255, 288
HindII GTYRAC 2 cut(s) 255, 288
HinfI GANTC 2 cut(s) 507, 565
HpaII CCGG 3 cut(s) 233, 543, 548
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 2 cut(s) 255, 288
Hpy188I TCNGA 5 cut(s) 117, 181, 506, 570, 636
Hpy188III TCNNGA 1 cut(s) 714
Hpy8I GTNNAC 2 cut(s) 255, 288
Hpy99I CGWCG 4 cut(s) 22, 256, 272, 308
HpyAV CCTTC 3 cut(s) 54, 391, 470
HpyCH4III ACNGT 3 cut(s) 165, 423, 428
HpyCH4V TGCA 4 cut(s) 214, 630, 648, 773
Hsp92I GRCGYC 2 cut(s) 17, 124
Hsp92II CATG 2 cut(s) 346, 538
HspAI GCGC 4 cut(s) 63, 199, 332, 671
KpnI GGTACC 1 cut(s) 427
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 2 cut(s) 284, 475
Lsp1109I GCAGC 5 cut(s) 198, 499, 511, 598, 632
LweI GCATC 4 cut(s) 487, 617, 657, 696
MaeI CTAG 2 cut(s) 455, 674
MaeIII GTNAC 1 cut(s) 439
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 4 cut(s) 95, 310, 358, 591
MhlI GDGCHC 1 cut(s) 281
MlsI TGGCCA 2 cut(s) 482, 702
MluCI AATT 2 cut(s) 371, 694
MluI ACGCGT 1 cut(s) 265
MluNI TGGCCA 2 cut(s) 482, 702
MlyI GAGTC 2 cut(s) 516, 574
Mox20I TGGCCA 2 cut(s) 482, 702
MscI TGGCCA 2 cut(s) 482, 702
MseI TTAA 1 cut(s) 790
Msp20I TGGCCA 2 cut(s) 482, 702
MspA1I CMGCKG 1 cut(s) 589
MspI CCGG 3 cut(s) 233, 543, 548
MspR9I CCNGG 3 cut(s) 196, 233, 543
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 2 cut(s) 267, 332
NciI CCSGG 2 cut(s) 233, 543
NdeII GATC 1 cut(s) 181
NheI GCTAGC 2 cut(s) 454, 673
NlaIII CATG 2 cut(s) 346, 538
NlaIV GGNNCC 3 cut(s) 7, 425, 490
NmuCI GTSAC 1 cut(s) 439
PaeR7I CTCGAG 1 cut(s) 91
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 6 cut(s) 99, 213, 514, 526, 588, 647
PleI GAGTC 2 cut(s) 515, 573
PpsI GAGTC 2 cut(s) 515, 573
PsiI TTATAA 1 cut(s) 779
Psp124BI GAGCTC 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 194
PspFI CCCAGC 2 cut(s) 515, 589
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 3 cut(s) 7, 425, 490
PspPI GGNCC 1 cut(s) 545
PspXI VCTCGAGB 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 2 cut(s) 416, 425
RsaNI GTAC 2 cut(s) 415, 424
Rsr2I CGGWCCG 1 cut(s) 545
RsrII CGGWCCG 1 cut(s) 545
SacI GAGCTC 1 cut(s) 281
SalI GTCGAC 1 cut(s) 253
SaqAI TTAA 1 cut(s) 790
SatI GCNGC 6 cut(s) 98, 212, 513, 525, 587, 646
Sau3AI GATC 1 cut(s) 181
Sau96I GGNCC 1 cut(s) 545
SchI GAGTC 2 cut(s) 516, 574
ScrFI CCNGG 3 cut(s) 196, 233, 543
SduI GDGCHC 1 cut(s) 281
SfaNI GCATC 4 cut(s) 487, 617, 657, 696
SfcI CTRYAG 1 cut(s) 660
Sfr274I CTCGAG 1 cut(s) 91
SinI GGWCC 1 cut(s) 545
SlaI CTCGAG 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
Sse9I AATT 2 cut(s) 371, 694
SsiI CCGC 3 cut(s) 98, 192, 687
SspI AATATT 1 cut(s) 411
SspMI CTAG 2 cut(s) 455, 674
SstI GAGCTC 1 cut(s) 281
StyD4I CCNGG 3 cut(s) 194, 231, 541
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 3 cut(s) 165, 423, 428
TaqI TCGA 2 cut(s) 92, 254
TaqII GACCGA 1 cut(s) 211
TasI AATT 2 cut(s) 371, 694
TatI WGTACW 1 cut(s) 414
TauI GCSGC 1 cut(s) 100
Tru1I TTAA 1 cut(s) 790
Tru9I TTAA 1 cut(s) 790
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 5 cut(s) 211, 512, 524, 586, 645
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 3 cut(s) 101, 359, 462
TspGWI ACGGA 2 cut(s) 240, 458
VpaK11BI GGWCC 1 cut(s) 545
XcmI CCANNNNNNNNNTGG 3 cut(s) 56, 490, 710
XhoI CTCGAG 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 254
XspI CTAG 2 cut(s) 455, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.