Rh7AG441400

CRT-like, chloroquine-resistance transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0003123
Physical Location & Seq
Forward (+)
59839732 .. 59845240
5509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG441400.1

Sequence Viewer

Length: 1281 bp
ATGGGTGCTTCCTCTACGTTGTCGTTTCACAAGTTCCTCTCCGTCCCCCCTTCTCTCTCTCCCCTCAAAAATGGCGGGACTTCCTTCCCTTCCTCCTCTCCGACCTCAATGCCACTCCTCCATAGGCCTCGCCCTTCTCTCCTCCGCCTCCACACTCCAAACGCACCGTTTCGCCTCCCCTCCGATCGCAAACCCACCGCCCGCAATGTCAAGCTCCACGCTTCACTCCCAAACCCTCCCCCCTCCTCCTCCGCGATCTCAAACGGCGTCGTTTTCAGCTCCGTGTTGACCATAGCCCTGGCCGTCGCCAACCGCGTTCTCTACAAGCTCGCACTCGTCCCCATGAAGAACCACCCCTTCTTTCTGGCCCAATTCATAACCTTTGGTTATGTGGTGATATATTTCTCAATATTGTACATACGGTACCGTTCTGGGATTGTGACTGACGAAATGCTAAGGCTTCCGAAATCGCGGTTCGTGGCCATCGGGGCCTTAGAGGCCCTTGGAGTCGCTTCTGGGATGGCTGCTGCAGCCATGCTTCCCGGACCGGCCATACCCATATTGAGTCAGACGTTTTTGGTGTGGCAGCTTGGTTTTTCTTCTCTTATCTTGGGAAGATCATACAGAATCAATCAGATTGTTGGCTGTATCCTCGTAGCTGCAGGTGTAGCGCTAGCTGTTGCTAGCGGGAACGATTCTGGCCAGATGCTATCTGGAGTTGAGTCTGTGTGGCCATTACTAATGATTTCTTCAAGTGCTTTTCAAGCTGGTGCATCTATAATCAAGGAATTTGTCTTTGTTGATGCTGCGGCCCACTTAAAGGGAAAATCACTTGACATATTTGTTGTCAACTCTTTCGGATCTGGATTTCAGGCTCTTTTTGTACTTCTATTTCTACCTTTCTTATCAAATATGAGAGGCATACCATTCGCCCAACTTCCTGCGTATCTAAAGGATGGTGCTGGTTGCTTTCTGAATATGGGAGCTGCTGCATCAGGTTGTAATGGGGCTCCATTGCTTCCGCTGCTTTACATAGCTGCCAATCTGTTGTTCAACATAAGCTTGCTGAATGTAGTAAAGATCTCCTCCGCGGTTGTTGCTTCTCTCATTGTGATGTTGTCAGTGCCAATCTCGATTTATGTTCTTTCCCTTCCATTGCCATATCTCCAAGAGGGTGCAACTTTGAGCCCCTTCTTCCTCTTTGGTAGTGCGGTTCTTGTCTTGGGTCTTATTTTGTACAGCATACCCCAACCTGCCAAGGATGTTTCTGAAATTAGTTGA

Protein Analysis

426

Amino Acids

45.14

Weight (kDa)

9.87

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 91 - 413 7.2e-37 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 653
Acc36I ACCTGC 2 cut(s) 653, 1261
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 1 cut(s) 423
AccII CGCG 4 cut(s) 254, 315, 472, 1091
AclWI GGATC 1 cut(s) 868
AcoI YGGCCR 5 cut(s) 300, 480, 549, 700, 731
AcsI RAATTY 1 cut(s) 788
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 4 cut(s) 416, 425, 885, 1238
AfeI AGCGCT 1 cut(s) 672
AfiI CCNNNNNNNGG 1 cut(s) 820
AgsI TTSAA 3 cut(s) 753, 764, 1054
AjnI CCWGG 1 cut(s) 297
AjuI GAANNNNNNNTTGG 2 cut(s) 1034, 1066
AlwI GGATC 1 cut(s) 868
Aor51HI AGCGCT 1 cut(s) 672
ApoI RAATTY 1 cut(s) 788
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 673
AspS9I GGNCC 5 cut(s) 367, 489, 499, 545, 811
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 1 cut(s) 406
AsuNHI GCTAGC 2 cut(s) 673, 683
AvaII GGWCC 1 cut(s) 545
BalI TGGCCA 3 cut(s) 482, 702, 733
BanI GGYRCC 1 cut(s) 423
BanII GRGCYC 2 cut(s) 1012, 1190
BccI CCATC 3 cut(s) 491, 514, 950
BceAI ACGGC 2 cut(s) 280, 287
BcgI CGANNNNNNTGC 4 cut(s) 91, 125, 910, 944
BciT130I CCWGG 1 cut(s) 299
BciVI GTATCC 1 cut(s) 659
BcnI CCSGG 1 cut(s) 543
BfaI CTAG 2 cut(s) 674, 684
BfmI CTRYAG 2 cut(s) 528, 660
BfoI RGCGCY 1 cut(s) 674
BfuAI ACCTGC 2 cut(s) 653, 1261
BfuI GTATCC 1 cut(s) 659
BglI GCCNNNNNGGC 2 cut(s) 488, 497
BglII AGATCT 1 cut(s) 1080
Bme1390I CCNGG 2 cut(s) 299, 543
Bme18I GGWCC 1 cut(s) 545
BmgT120I GGNCC 5 cut(s) 367, 489, 499, 545, 811
BmiI GGNNCC 3 cut(s) 425, 490, 1011
BmrFI CCNGG 2 cut(s) 299, 543
BmsI GCATC 4 cut(s) 696, 782, 793, 1001
BmtI GCTAGC 2 cut(s) 677, 687
BpmI CTGGAG 1 cut(s) 735
Bpu10I CCTNAGC 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 297, 502, 1089, 1257
Bsc4I CCNNNNNNNGG 1 cut(s) 820
Bse118I RCCGGY 1 cut(s) 547
Bse3DI GCAATG 3 cut(s) 211, 1013, 1154
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 4 cut(s) 297, 502, 1089, 1257
BseGI GGATG 3 cut(s) 525, 961, 1267
BseLI CCNNNNNNNGG 1 cut(s) 820
BseMI GCAATG 3 cut(s) 211, 1013, 1154
BseRI GAGGAG 6 cut(s) 85, 107, 131, 235, 238, 1075
Bsh1236I CGCG 4 cut(s) 254, 315, 472, 1091
Bsh1285I CGRYCG 1 cut(s) 187
BshNI GGYRCC 1 cut(s) 423
BsiEI CGRYCG 1 cut(s) 187
BsiSI CCGG 2 cut(s) 543, 548
BslFI GGGAC 3 cut(s) 29, 91, 323
BslI CCNNNNNNNGG 1 cut(s) 820
BsmFI GGGAC 3 cut(s) 29, 91, 323
Bsp1286I GDGCHC 2 cut(s) 1012, 1190
Bsp1407I TGTACA 2 cut(s) 414, 1236
Bsp143I GATC 5 cut(s) 184, 255, 617, 860, 1080
BspFNI CGCG 4 cut(s) 254, 315, 472, 1091
BspLI GGNNCC 3 cut(s) 425, 490, 1011
BspMAI CTGCAG 2 cut(s) 532, 664
BspMI ACCTGC 2 cut(s) 653, 1261
BspOI GCTAGC 2 cut(s) 677, 687
BspPI GGATC 1 cut(s) 868
BspT107I GGYRCC 1 cut(s) 423
BsrDI GCAATG 3 cut(s) 211, 1013, 1154
BsrFI RCCGGY 1 cut(s) 547
BsrGI TGTACA 2 cut(s) 414, 1236
BssAI RCCGGY 1 cut(s) 547
BssECI CCNNGG 4 cut(s) 297, 502, 1089, 1257
BssMI GATC 5 cut(s) 184, 255, 617, 860, 1080
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 2 cut(s) 502, 1257
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 3 cut(s) 168, 423, 428
BstACI GRCGYC 1 cut(s) 267
BstAUI TGTACA 2 cut(s) 414, 1236
BstC8I GCNNGC 5 cut(s) 202, 330, 675, 685, 1064
BstDEI CTNAG 2 cut(s) 455, 493
BstDSI CCRYGG 1 cut(s) 1089
BstF5I GGATG 3 cut(s) 525, 961, 1267
BstFNI CGCG 4 cut(s) 254, 315, 472, 1091
BstH2I RGCGCY 1 cut(s) 674
BstHHI GCGC 1 cut(s) 673
BstKTI GATC 5 cut(s) 187, 258, 620, 863, 1083
BstMBI GATC 5 cut(s) 184, 255, 617, 860, 1080
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 7 cut(s) 488, 497, 530, 668, 764, 1024, 1097
BstNI CCWGG 1 cut(s) 299
BstSCI CCNGG 2 cut(s) 297, 541
BstSFI CTRYAG 2 cut(s) 528, 660
BstUI CGCG 4 cut(s) 254, 315, 472, 1091
BstX2I RGATCY 2 cut(s) 860, 1080
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 2 cut(s) 860, 1080
BsuI GTATCC 1 cut(s) 659
BtgI CCRYGG 1 cut(s) 1089
BtsCI GGATG 3 cut(s) 525, 961, 1267
BtsIMutI CAGTG 1 cut(s) 1128
BveI ACCTGC 2 cut(s) 653, 1261
Cac8I GCNNGC 5 cut(s) 202, 330, 675, 685, 1064
CfoI GCGC 1 cut(s) 673
Cfr10I RCCGGY 1 cut(s) 547
Cfr13I GGNCC 5 cut(s) 367, 489, 499, 545, 811
Cfr42I CCGCGG 1 cut(s) 1092
CpoI CGGWCCG 1 cut(s) 545
CseI GACGC 1 cut(s) 256
Csp6I GTAC 4 cut(s) 415, 424, 884, 1237
CspI CGGWCCG 1 cut(s) 545
CviAII CATG 2 cut(s) 343, 535
CviQI GTAC 4 cut(s) 415, 424, 884, 1237
DdeI CTNAG 2 cut(s) 455, 493
DpnI GATC 5 cut(s) 186, 257, 619, 862, 1082
DpnII GATC 5 cut(s) 184, 255, 617, 860, 1080
EaeI YGGCCR 5 cut(s) 300, 480, 549, 700, 731
EciI GGCGGA 1 cut(s) 134
Eco130I CCWWGG 2 cut(s) 502, 1257
Eco147I AGGCCT 1 cut(s) 127
Eco24I GRGCYC 2 cut(s) 1012, 1190
Eco47I GGWCC 1 cut(s) 545
Eco47III AGCGCT 1 cut(s) 672
EcoO109I RGGNCCY 2 cut(s) 489, 499
EcoRII CCWGG 1 cut(s) 297
EcoT14I CCWWGG 2 cut(s) 502, 1257
EcoT38I GRGCYC 2 cut(s) 1012, 1190
ErhI CCWWGG 2 cut(s) 502, 1257
FaeI CATG 2 cut(s) 346, 538
FaqI GGGAC 3 cut(s) 29, 91, 323
FatI CATG 2 cut(s) 342, 534
FauI CCCGC 3 cut(s) 68, 209, 680
FokI GGATG 3 cut(s) 532, 968, 1274
FriOI GRGCYC 2 cut(s) 1012, 1190
FspBI CTAG 2 cut(s) 674, 684
GlaI GCGC 1 cut(s) 672
GsuI CTGGAG 1 cut(s) 735
HaeII RGCGCY 1 cut(s) 674
HapII CCGG 2 cut(s) 543, 548
HgaI GACGC 1 cut(s) 256
HhaI GCGC 1 cut(s) 673
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 2 cut(s) 346, 538
Hin6I GCGC 1 cut(s) 671
HinP1I GCGC 1 cut(s) 671
HincII GTYRAC 2 cut(s) 288, 850
HindII GTYRAC 2 cut(s) 288, 850
HindIII AAGCTT 1 cut(s) 1060
HinfI GANTC 5 cut(s) 507, 565, 627, 695, 722
HpaII CCGG 2 cut(s) 543, 548
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 2 cut(s) 288, 850
Hpy188I TCNGA 8 cut(s) 102, 184, 465, 570, 636, 860, 975, 1270
Hpy188III TCNNGA 3 cut(s) 714, 864, 1132
Hpy8I GTNNAC 2 cut(s) 288, 850
Hpy99I CGWCG 2 cut(s) 272, 308
HpyAV CCTTC 7 cut(s) 60, 94, 99, 144, 367, 1160, 1201
HpyCH4III ACNGT 3 cut(s) 168, 423, 428
HpyCH4IV ACGT 2 cut(s) 17, 572
HpyCH4V TGCA 5 cut(s) 530, 662, 773, 992, 1178
HpyF10VI GCNNNNNNNGC 7 cut(s) 488, 497, 530, 668, 764, 1024, 1097
HpyF3I CTNAG 2 cut(s) 455, 493
HpySE526I ACGT 2 cut(s) 17, 572
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 2 cut(s) 346, 538
HspAI GCGC 1 cut(s) 671
KpnI GGTACC 1 cut(s) 427
KspI CCGCGG 1 cut(s) 1092
Kzo9I GATC 5 cut(s) 184, 255, 617, 860, 1080
LmnI GCTCC 4 cut(s) 219, 284, 983, 1015
LweI GCATC 4 cut(s) 696, 782, 793, 1001
MaeI CTAG 2 cut(s) 674, 684
MaeII ACGT 2 cut(s) 17, 572
MaeIII GTNAC 1 cut(s) 439
MalI GATC 5 cut(s) 186, 257, 619, 862, 1082
MboI GATC 5 cut(s) 184, 255, 617, 860, 1080
MboII GAAGA 5 cut(s) 358, 591, 627, 741, 1186
MflI RGATCY 2 cut(s) 860, 1080
MhlI GDGCHC 2 cut(s) 1012, 1190
MlsI TGGCCA 3 cut(s) 482, 702, 733
MluCI AATT 3 cut(s) 371, 788, 1272
MluNI TGGCCA 3 cut(s) 482, 702, 733
MlyI GAGTC 3 cut(s) 516, 574, 731
MmeI TCCRAC 1 cut(s) 125
Mox20I TGGCCA 3 cut(s) 482, 702, 733
MscI TGGCCA 3 cut(s) 482, 702, 733
MseI TTAA 1 cut(s) 818
MslI CAYNNNNRTG 1 cut(s) 1112
Msp20I TGGCCA 3 cut(s) 482, 702, 733
MspA1I CMGCKG 2 cut(s) 1024, 1091
MspI CCGG 2 cut(s) 543, 548
MspR9I CCNGG 2 cut(s) 299, 543
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 4 cut(s) 254, 315, 472, 1091
MwoI GCNNNNNNNGC 7 cut(s) 488, 497, 530, 668, 764, 1024, 1097
NciI CCSGG 1 cut(s) 543
NdeII GATC 5 cut(s) 184, 255, 617, 860, 1080
NheI GCTAGC 2 cut(s) 673, 683
NlaIII CATG 2 cut(s) 346, 538
NlaIV GGNNCC 3 cut(s) 425, 490, 1011
NmuCI GTSAC 1 cut(s) 439
PaqCI CACCTGC 1 cut(s) 653
PceI AGGCCT 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfeI GAWTC 2 cut(s) 627, 695
PfoI TCCNGGA 1 cut(s) 541
Ple19I CGATCG 1 cut(s) 187
PleI GAGTC 3 cut(s) 515, 573, 730
PpsI GAGTC 3 cut(s) 515, 573, 730
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 425, 490, 1011
PspPI GGNCC 5 cut(s) 367, 489, 499, 545, 811
PstI CTGCAG 2 cut(s) 532, 664
PsuI RGATCY 2 cut(s) 860, 1080
PvuI CGATCG 1 cut(s) 187
RsaI GTAC 4 cut(s) 416, 425, 885, 1238
RsaNI GTAC 4 cut(s) 415, 424, 884, 1237
RseI CAYNNNNRTG 1 cut(s) 1112
Rsr2I CGGWCCG 1 cut(s) 545
RsrII CGGWCCG 1 cut(s) 545
SacII CCGCGG 1 cut(s) 1092
SaqAI TTAA 1 cut(s) 818
Sau3AI GATC 5 cut(s) 184, 255, 617, 860, 1080
Sau96I GGNCC 5 cut(s) 367, 489, 499, 545, 811
SchI GAGTC 3 cut(s) 516, 574, 731
ScrFI CCNGG 2 cut(s) 299, 543
SduI GDGCHC 2 cut(s) 1012, 1190
SfaNI GCATC 4 cut(s) 696, 782, 793, 1001
SfcI CTRYAG 2 cut(s) 528, 660
SfiI GGCCNNNNNGGCC 2 cut(s) 488, 497
Sfr303I CCGCGG 1 cut(s) 1092
SgrBI CCGCGG 1 cut(s) 1092
SinI GGWCC 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 1112
Sse9I AATT 3 cut(s) 371, 788, 1272
SseBI AGGCCT 1 cut(s) 127
SspI AATATT 1 cut(s) 411
SspMI CTAG 2 cut(s) 674, 684
StuI AGGCCT 1 cut(s) 127
StyD4I CCNGG 2 cut(s) 297, 541
StyI CCWWGG 2 cut(s) 502, 1257
TaaI ACNGT 3 cut(s) 168, 423, 428
TaiI ACGT 2 cut(s) 20, 575
TaqI TCGA 1 cut(s) 1133
TasI AATT 3 cut(s) 371, 788, 1272
TatI WGTACW 3 cut(s) 414, 883, 1236
TauI GCSGC 1 cut(s) 812
TfiI GAWTC 2 cut(s) 627, 695
Tru1I TTAA 1 cut(s) 818
Tru9I TTAA 1 cut(s) 818
TscAI CASTG 1 cut(s) 1128
TseFI GTSAC 1 cut(s) 439
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 2 cut(s) 359, 364
TspGWI ACGGA 2 cut(s) 31, 271
TspRI CASTG 1 cut(s) 1128
VpaK11BI GGWCC 1 cut(s) 545
XapI RAATTY 1 cut(s) 788
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XspI CTAG 2 cut(s) 674, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.