AT4G28440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003987
Physical Location & Seq
Forward (+)
14059896 .. 14061386
1491 bp
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UTR
Exon/CDS
Intron
AT4G28440.1

Sequence Viewer

Length: 462 bp
ATGGCGACGACTGGAACTGCTGCGGTTGCGACGGGAACTTCGACGGTGAAGAGAAAACCGGTGTTTGTGAAGGTGGAACAGCTAAAGCCTGGAACGACTGGTCACACGTTGACTGTTAAAGTCATTGAGGCTAATATCGTGGTTCCTGTTACCCGGAAGACTCGTCCAGCGAGTTCTCTGAGTCGTCCTTCTCAGCCTAGTCGAATCGTTGAGTGTCTCATCGGTGACGAAACTGGGTGTATCCTCTTCACTGCTCGTAACGATCAAGTTGATCTTATGAAGCCGGGTGCTACGGTGATACTGCGCAATTCAAGGATAGACATGTTCAAGGGTACAATGAGGCTAGGAGTTGATAAATGGGGACGCATCGAAGCCACTGGAGCCGCGTCTTTCACTGTCAAGGAGGATAACAATCTGTCTCTCGTTGAGTATGAACTGATCAATGTTGGTGGTGATCAGTGA

Protein Analysis

153

Amino Acids

16.46

Weight (kDa)

9.47

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 28 - 125 1.7e-47 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 305
AccII CGCG 1 cut(s) 386
AciI CCGC 2 cut(s) 23, 384
AfaI GTAC 1 cut(s) 334
AflIII ACRYGT 2 cut(s) 105, 321
AgeI ACCGGT 1 cut(s) 58
AgsI TTSAA 2 cut(s) 312, 328
AjnI CCWGG 1 cut(s) 88
AluBI AGCT 1 cut(s) 82
AluI AGCT 1 cut(s) 82
Alw26I GTCTC 2 cut(s) 221, 423
ApeKI GCWGC 1 cut(s) 20
ArsI GACNNNNNNTTYG 2 cut(s) 22, 54
AsiGI ACCGGT 1 cut(s) 58
AspLEI GCGC 1 cut(s) 306
AsuC2I CCSGG 2 cut(s) 154, 285
AsuHPI GGTGA 3 cut(s) 58, 236, 307
BbsI GAAGAC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 7
BciT130I CCWGG 1 cut(s) 90
BciVI GTATCC 1 cut(s) 251
BclI TGATCA 2 cut(s) 438, 454
BcnI CCSGG 2 cut(s) 154, 285
BcoDI GTCTC 2 cut(s) 221, 423
BfaI CTAG 2 cut(s) 198, 344
BfuI GTATCC 1 cut(s) 251
BisI GCNGC 2 cut(s) 21, 384
BlsI GCNGC 2 cut(s) 22, 385
Bme1390I CCNGG 3 cut(s) 90, 154, 285
BmiI GGNNCC 2 cut(s) 144, 382
BmrFI CCNGG 3 cut(s) 90, 154, 285
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 1 cut(s) 375
BmuI ACTGGG 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 164
BpmI CTGGAG 1 cut(s) 399
BpuMI CCSGG 2 cut(s) 154, 285
BsaBI GATNNNNATC 1 cut(s) 411
BsaWI WCCGGW 1 cut(s) 58
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 4 cut(s) 16, 103, 238, 382
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 1 cut(s) 90
BseJI GATNNNNATC 1 cut(s) 411
BseMII CTCAG 2 cut(s) 170, 206
BseNI ACTGG 4 cut(s) 16, 103, 238, 382
BseXI GCAGC 1 cut(s) 7
Bsh1236I CGCG 1 cut(s) 386
BshTI ACCGGT 1 cut(s) 58
BsiSI CCGG 3 cut(s) 59, 154, 284
BslFI GGGAC 1 cut(s) 375
BsmAI GTCTC 2 cut(s) 221, 423
BsmFI GGGAC 1 cut(s) 375
Bsp143I GATC 4 cut(s) 262, 271, 438, 454
BspACI CCGC 2 cut(s) 23, 384
BspCNI CTCAG 2 cut(s) 171, 205
BspFNI CGCG 1 cut(s) 386
BspLI GGNNCC 2 cut(s) 144, 382
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 4 cut(s) 16, 103, 238, 382
BssAI RCCGGY 1 cut(s) 58
BssMI GATC 4 cut(s) 262, 271, 438, 454
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 4 cut(s) 46, 115, 295, 397
Bst6I CTCTTC 2 cut(s) 44, 251
BstDEI CTNAG 2 cut(s) 179, 192
BstFNI CGCG 1 cut(s) 386
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 4 cut(s) 265, 274, 441, 457
BstMAI GTCTC 2 cut(s) 221, 423
BstMBI GATC 4 cut(s) 262, 271, 438, 454
BstMWI GCNNNNNNNGC 2 cut(s) 26, 380
BstNI CCWGG 1 cut(s) 90
BstNSI RCATGY 1 cut(s) 325
BstSCI CCNGG 3 cut(s) 88, 152, 283
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 1 cut(s) 7
BstV2I GAAGAC 1 cut(s) 164
BsuI GTATCC 1 cut(s) 251
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 3 cut(s) 249, 375, 393
CfoI GCGC 1 cut(s) 306
Cfr10I RCCGGY 1 cut(s) 58
CseI GACGC 2 cut(s) 372, 375
Csp6I GTAC 1 cut(s) 333
CspAI ACCGGT 1 cut(s) 58
CspCI CAANNNNNGTGG 1 cut(s) 430
CviAII CATG 1 cut(s) 322
CviJI RGCY 8 cut(s) 82, 88, 131, 196, 283, 343, 374, 383
CviKI_1 RGCY 8 cut(s) 82, 88, 131, 196, 283, 343, 374, 383
CviQI GTAC 1 cut(s) 333
DdeI CTNAG 2 cut(s) 179, 192
DpnI GATC 4 cut(s) 264, 273, 440, 456
DpnII GATC 4 cut(s) 262, 271, 438, 454
Eam1104I CTCTTC 2 cut(s) 44, 251
EarI CTCTTC 2 cut(s) 44, 251
EcoRII CCWGG 1 cut(s) 88
FaeI CATG 1 cut(s) 325
FaiI YATR 3 cut(s) 278, 323, 432
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FaqI GGGAC 1 cut(s) 375
FatI CATG 1 cut(s) 321
FbaI TGATCA 2 cut(s) 438, 454
Fnu4HI GCNGC 2 cut(s) 21, 384
Fsp4HI GCNGC 2 cut(s) 21, 384
FspBI CTAG 2 cut(s) 198, 344
FspI TGCGCA 1 cut(s) 305
GlaI GCGC 1 cut(s) 305
GluI GCNGC 2 cut(s) 21, 384
GsuI CTGGAG 1 cut(s) 399
HapII CCGG 3 cut(s) 59, 154, 284
HgaI GACGC 2 cut(s) 372, 375
HhaI GCGC 1 cut(s) 306
Hin1II CATG 1 cut(s) 325
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HinfI GANTC 3 cut(s) 160, 181, 204
HpaII CCGG 3 cut(s) 59, 154, 284
HphI GGTGA 3 cut(s) 58, 236, 307
Hpy166II GTNNAC 1 cut(s) 111
Hpy188I TCNGA 1 cut(s) 180
Hpy8I GTNNAC 1 cut(s) 111
Hpy99I CGWCG 3 cut(s) 10, 34, 46
HpyAV CCTTC 2 cut(s) 64, 198
HpyCH4III ACNGT 4 cut(s) 46, 115, 295, 397
HpyCH4IV ACGT 1 cut(s) 107
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 380
HpyF3I CTNAG 2 cut(s) 179, 192
HpySE526I ACGT 1 cut(s) 107
Hsp92II CATG 1 cut(s) 325
HspAI GCGC 1 cut(s) 304
Ksp22I TGATCA 2 cut(s) 438, 454
Kzo9I GATC 4 cut(s) 262, 271, 438, 454
LmnI GCTCC 1 cut(s) 380
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 1 cut(s) 375
MaeI CTAG 2 cut(s) 198, 344
MaeII ACGT 1 cut(s) 107
MaeIII GTNAC 4 cut(s) 101, 148, 224, 257
MalI GATC 4 cut(s) 264, 273, 440, 456
MboI GATC 4 cut(s) 262, 271, 438, 454
MboII GAAGA 3 cut(s) 61, 169, 238
MluCI AATT 1 cut(s) 307
MlyI GAGTC 2 cut(s) 154, 190
MnlI CCTC 4 cut(s) 121, 254, 333, 397
MseI TTAA 1 cut(s) 117
MspI CCGG 3 cut(s) 59, 154, 284
MspR9I CCNGG 3 cut(s) 90, 154, 285
MvaI CCWGG 1 cut(s) 90
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 2 cut(s) 26, 380
NciI CCSGG 2 cut(s) 154, 285
NdeII GATC 4 cut(s) 262, 271, 438, 454
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 2 cut(s) 144, 382
NmuCI GTSAC 2 cut(s) 101, 224
NsbI TGCGCA 1 cut(s) 305
NspI RCATGY 1 cut(s) 325
PciI ACATGT 1 cut(s) 321
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PfeI GAWTC 1 cut(s) 204
PinAI ACCGGT 1 cut(s) 58
PkrI GCNGC 2 cut(s) 22, 385
PleI GAGTC 2 cut(s) 154, 189
PpsI GAGTC 2 cut(s) 154, 189
PscI ACATGT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 88
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 2 cut(s) 144, 382
RsaI GTAC 1 cut(s) 334
RsaNI GTAC 1 cut(s) 333
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 2 cut(s) 21, 384
Sau3AI GATC 4 cut(s) 262, 271, 438, 454
SchI GAGTC 2 cut(s) 154, 190
ScrFI CCNGG 3 cut(s) 90, 154, 285
SetI ASST 3 cut(s) 75, 84, 110
SfaNI GCATC 1 cut(s) 375
Sse9I AATT 1 cut(s) 307
SsiI CCGC 2 cut(s) 23, 384
SspMI CTAG 2 cut(s) 198, 344
StyD4I CCNGG 3 cut(s) 88, 152, 283
TaaI ACNGT 4 cut(s) 46, 115, 295, 397
TaiI ACGT 1 cut(s) 110
TaqI TCGA 3 cut(s) 41, 202, 369
TasI AATT 1 cut(s) 307
TauI GCSGC 1 cut(s) 386
TfiI GAWTC 1 cut(s) 204
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 3 cut(s) 256, 382, 400
TseFI GTSAC 2 cut(s) 101, 224
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 2 cut(s) 101, 224
TspDTI ATGAA 2 cut(s) 293, 447
TspRI CASTG 3 cut(s) 256, 382, 400
XceI RCATGY 1 cut(s) 325
XspI CTAG 2 cut(s) 198, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.