Rh5BG346800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003987
Physical Location & Seq
Forward (+)
52998137 .. 52999466
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG346800.1

Sequence Viewer

Length: 363 bp
ATGGCGGTGCTCGATCCTCTCTCTCGTCTCTCCCTCTTCAGCCCCCTCGCATCACTGAGTGCCTCGTTAGCGATGAGACTGGAACCATTGTCTTCACTGCTCGCAATGACCAAGCCTAGTACCACTGTGATTCCACGCAATACAAAGATTGACATGTTCATCAAAGGCACTATGAGGCTTGCAGTTGATAAATGGGGTTGTGTTGAAGTCACAGAACCAGCCAGCTTTGAAGTTAAAGAGGTGAAAAGCGGAAAAAGTAGCTTAGAGCAGATATCTAGAGTTTACGGAAATCAGGTAATATCTTTGTCTAGTTTTAGGTGCATGCCCATAATGCTTCAATTCATTTATATTATGTTGAGATGA

Protein Analysis

120

Amino Acids

13.35

Weight (kDa)

9.56

Isoelectric Point (pI)

28.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 36 - 70 3.6e-10 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 249
AclWI GGATC 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 22
AdeI CACNNNGTG 1 cut(s) 59
AfaI GTAC 1 cut(s) 121
AflIII ACRYGT 1 cut(s) 153
AgsI TTSAA 3 cut(s) 206, 230, 338
AluBI AGCT 2 cut(s) 225, 261
AluI AGCT 2 cut(s) 225, 261
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 2 cut(s) 32, 70
AlwI GGATC 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 32, 70
BfaI CTAG 3 cut(s) 117, 276, 309
BmiI GGNNCC 1 cut(s) 84
BmsI GCATC 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 84
Bse1I ACTGG 1 cut(s) 84
Bse3DI GCAATG 1 cut(s) 111
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 12
BsmAI GTCTC 2 cut(s) 32, 70
BsmBI CGTCTC 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 12
Bsp143I GATC 1 cut(s) 13
BspACI CCGC 2 cut(s) 5, 249
BspCNI CTCAG 1 cut(s) 48
BspLI GGNNCC 1 cut(s) 84
BspPI GGATC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 111
BsrI ACTGG 1 cut(s) 84
BssMI GATC 1 cut(s) 13
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 1 cut(s) 41
BstC8I GCNNGC 4 cut(s) 102, 180, 223, 323
BstDEI CTNAG 2 cut(s) 56, 262
BstKTI GATC 1 cut(s) 16
BstMAI GTCTC 2 cut(s) 32, 70
BstMBI GATC 1 cut(s) 13
BstMWI GCNNNNNNNGC 2 cut(s) 68, 331
BstNSI RCATGY 2 cut(s) 157, 325
BstV2I GAAGAC 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 86
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 3 cut(s) 53, 95, 123
Cac8I GCNNGC 4 cut(s) 102, 180, 223, 323
Csp6I GTAC 1 cut(s) 120
CviAII CATG 2 cut(s) 154, 322
CviJI RGCY 6 cut(s) 42, 115, 178, 221, 225, 261
CviKI_1 RGCY 6 cut(s) 42, 115, 178, 221, 225, 261
CviQI GTAC 1 cut(s) 120
DdeI CTNAG 2 cut(s) 56, 262
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
DraIII CACNNNGTG 1 cut(s) 59
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco32I GATATC 1 cut(s) 273
Eco57I CTGAAG 1 cut(s) 22
EcoRV GATATC 1 cut(s) 273
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 2 cut(s) 157, 325
FaiI YATR 6 cut(s) 155, 173, 323, 329, 348, 353
FatI CATG 2 cut(s) 153, 321
FspBI CTAG 3 cut(s) 117, 276, 309
Hin1II CATG 2 cut(s) 157, 325
HinfI GANTC 1 cut(s) 130
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 1 cut(s) 283
Hpy188III TCNNGA 1 cut(s) 276
Hpy8I GTNNAC 1 cut(s) 283
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4V TGCA 2 cut(s) 182, 321
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 331
HpyF3I CTNAG 2 cut(s) 56, 262
Hsp92II CATG 2 cut(s) 157, 325
Kzo9I GATC 1 cut(s) 13
LpnPI CCDG 4 cut(s) 65, 231, 235, 278
LweI GCATC 1 cut(s) 59
MaeI CTAG 3 cut(s) 117, 276, 309
MaeIII GTNAC 1 cut(s) 208
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 2 cut(s) 28, 84
MhlI GDGCHC 1 cut(s) 12
MluCI AATT 1 cut(s) 338
MnlI CCTC 6 cut(s) 27, 44, 56, 73, 168, 232
MseI TTAA 1 cut(s) 234
MwoI GCNNNNNNNGC 2 cut(s) 68, 331
NdeII GATC 1 cut(s) 13
NlaIII CATG 2 cut(s) 157, 325
NlaIV GGNNCC 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 2 cut(s) 157, 325
PaeI GCATGC 1 cut(s) 325
PciI ACATGT 1 cut(s) 153
PfeI GAWTC 1 cut(s) 130
PscI ACATGT 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 84
RsaI GTAC 1 cut(s) 121
RsaNI GTAC 1 cut(s) 120
SaqAI TTAA 1 cut(s) 234
Sau3AI GATC 1 cut(s) 13
SduI GDGCHC 1 cut(s) 12
SetI ASST 5 cut(s) 227, 243, 263, 297, 320
SfaNI GCATC 1 cut(s) 59
SphI GCATGC 1 cut(s) 325
Sse9I AATT 1 cut(s) 338
SsiI CCGC 2 cut(s) 5, 249
SspMI CTAG 3 cut(s) 117, 276, 309
TaaI ACNGT 1 cut(s) 127
TaqI TCGA 1 cut(s) 12
TasI AATT 1 cut(s) 338
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 3 cut(s) 60, 102, 130
TseFI GTSAC 1 cut(s) 208
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 148, 331
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 3 cut(s) 60, 102, 130
XbaI TCTAGA 1 cut(s) 275
XceI RCATGY 2 cut(s) 157, 325
XspI CTAG 3 cut(s) 117, 276, 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.