MD09G1242500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003987
Physical Location & Seq
Reverse (-)
30965994 .. 30967702
1709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1242500.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGCGACGACGACAGCGAATAATTTAGGGACAGTGACGGCGAAGAGAAAGCCGGTGTTCGTAAAGGTGGAGCAGCTCCAGCCAAACACGAACGGCCACACCCTCACGGTGAAGGTGGTGAGCTCGAAGCCGGTGAAGGTCACCAACAAGAACGGCGGTCGATCATCTTTGTCGTCTCGGCCTACTCAGCCCGCTCGCATCGCTGAGTGCCTCGTTGGGGACGAGACGGGGACCATTCTCTTCACCGCTCGCAATGACCAAGTTGACACTATGAAGCCAGACACGACTGTGAACCTGCGTAATGCAAAGATTGACATGTTCAAGGGGACCATGAGGCTAGCAGTCGACAAATGGGGACGTGTTGAAGTCACCGAACCAGCTAACTTCCAAGTTAAAGAGGATAACAATCTTTCTCTCATTGAGTACGAGTTGGTTAACGTTGAAGAGTGA

Protein Analysis

150

Amino Acids

16.42

Weight (kDa)

9.1

Isoelectric Point (pI)

28.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 26 - 123 1.5e-46 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 303
AccBSI CCGCTC 2 cut(s) 194, 248
AccI GTMKAC 1 cut(s) 345
AciI CCGC 3 cut(s) 156, 192, 246
AclI AACGTT 1 cut(s) 438
AcoI YGGCCR 1 cut(s) 94
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 1 cut(s) 217
AflIII ACRYGT 2 cut(s) 315, 358
AgsI TTSAA 3 cut(s) 322, 365, 443
AjiI CACGTC 1 cut(s) 359
AleI CACNNNNGTG 1 cut(s) 287
AluBI AGCT 3 cut(s) 76, 123, 380
AluI AGCT 3 cut(s) 76, 123, 380
Alw21I GWGCWC 1 cut(s) 125
Alw26I GTCTC 2 cut(s) 180, 218
AoxI GGCC 2 cut(s) 94, 179
ApeKI GCWGC 1 cut(s) 73
AspS9I GGNCC 2 cut(s) 231, 327
AsuHPI GGTGA 6 cut(s) 121, 130, 133, 145, 235, 361
AsuNHI GCTAGC 1 cut(s) 337
AvaII GGWCC 2 cut(s) 231, 327
BanII GRGCYC 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 125
BbvI GCAGC 1 cut(s) 85
BceAI ACGGC 3 cut(s) 54, 109, 169
BcoDI GTCTC 2 cut(s) 180, 218
BfaI CTAG 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 303
BisI GCNGC 1 cut(s) 74
BlsI GCNGC 1 cut(s) 75
Bme18I GGWCC 2 cut(s) 231, 327
BmgBI CACGTC 1 cut(s) 359
BmgT120I GGNCC 2 cut(s) 231, 327
BmiI GGNNCC 2 cut(s) 232, 328
BmsI GCATC 1 cut(s) 207
BmtI GCTAGC 1 cut(s) 341
BpmI CTGGAG 1 cut(s) 62
BsaBI GATNNNNATC 1 cut(s) 405
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 2 cut(s) 52, 130
Bse3DI GCAATG 1 cut(s) 259
Bse8I GATNNNNATC 1 cut(s) 405
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 2 cut(s) 195, 200
BseXI GCAGC 1 cut(s) 85
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 2 cut(s) 96, 181
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 125
BsiSI CCGG 2 cut(s) 53, 131
BslFI GGGAC 5 cut(s) 43, 233, 244, 340, 369
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 2 cut(s) 180, 218
BsmBI CGTCTC 2 cut(s) 180, 218
BsmFI GGGAC 5 cut(s) 43, 233, 244, 340, 369
BsnI GGCC 2 cut(s) 96, 181
Bsp1286I GDGCHC 1 cut(s) 125
Bsp143I GATC 1 cut(s) 161
BspACI CCGC 3 cut(s) 156, 192, 246
BspANI GGCC 2 cut(s) 96, 181
BspCNI CTCAG 2 cut(s) 196, 199
BspLI GGNNCC 2 cut(s) 232, 328
BspMI ACCTGC 1 cut(s) 303
BspOI GCTAGC 1 cut(s) 341
BsrBI CCGCTC 2 cut(s) 194, 248
BsrDI GCAATG 1 cut(s) 259
BsrFI RCCGGY 2 cut(s) 52, 130
BssAI RCCGGY 2 cut(s) 52, 130
BssMI GATC 1 cut(s) 161
Bst4CI ACNGT 3 cut(s) 34, 109, 289
Bst6I CTCTTC 3 cut(s) 38, 245, 438
BstC8I GCNNGC 4 cut(s) 192, 196, 250, 339
BstDEI CTNAG 2 cut(s) 186, 204
BstEII GGTNACC 1 cut(s) 139
BstKTI GATC 1 cut(s) 164
BstMAI GTCTC 2 cut(s) 180, 218
BstMBI GATC 1 cut(s) 161
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 3 cut(s) 79, 187, 200
BstNSI RCATGY 1 cut(s) 319
BstPI GGTNACC 1 cut(s) 139
BstV1I GCAGC 1 cut(s) 85
BsuRI GGCC 2 cut(s) 96, 181
BtgZI GCGATG 1 cut(s) 184
BtrI CACGTC 1 cut(s) 359
BtsIMutI CAGTG 1 cut(s) 39
BveI ACCTGC 1 cut(s) 303
Cac8I GCNNGC 4 cut(s) 192, 196, 250, 339
Cfr10I RCCGGY 2 cut(s) 52, 130
Cfr13I GGNCC 2 cut(s) 231, 327
Csp6I GTAC 1 cut(s) 424
CviAII CATG 2 cut(s) 316, 331
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 2 cut(s) 186, 204
DpnI GATC 1 cut(s) 163
DpnII GATC 1 cut(s) 161
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 3 cut(s) 38, 245, 438
EarI CTCTTC 3 cut(s) 38, 245, 438
Ecl136II GAGCTC 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 125
Eco47I GGWCC 2 cut(s) 231, 327
Eco53kI GAGCTC 1 cut(s) 123
Eco91I GGTNACC 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 123
EcoO65I GGTNACC 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 125
Esp3I CGTCTC 2 cut(s) 180, 218
FaeI CATG 2 cut(s) 319, 334
FaiI YATR 3 cut(s) 272, 317, 332
FaqI GGGAC 5 cut(s) 43, 233, 244, 340, 369
FatI CATG 2 cut(s) 315, 330
FauI CCCGC 1 cut(s) 199
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 1 cut(s) 74
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 1 cut(s) 74
FspBI CTAG 1 cut(s) 338
GluI GCNGC 1 cut(s) 74
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 2 cut(s) 96, 181
HapII CCGG 2 cut(s) 53, 131
Hin1II CATG 2 cut(s) 319, 334
HincII GTYRAC 3 cut(s) 265, 346, 436
HindII GTYRAC 3 cut(s) 265, 346, 436
HpaI GTTAAC 1 cut(s) 436
HpaII CCGG 2 cut(s) 53, 131
HphI GGTGA 6 cut(s) 121, 130, 133, 145, 235, 361
Hpy166II GTNNAC 4 cut(s) 265, 292, 346, 436
Hpy8I GTNNAC 4 cut(s) 265, 292, 346, 436
Hpy99I CGWCG 2 cut(s) 10, 13
HpyAV CCTTC 2 cut(s) 106, 130
HpyCH4III ACNGT 3 cut(s) 34, 109, 289
HpyCH4IV ACGT 2 cut(s) 358, 438
HpyCH4V TGCA 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 187, 200
HpyF3I CTNAG 2 cut(s) 186, 204
HpySE526I ACGT 2 cut(s) 358, 438
Hsp92II CATG 2 cut(s) 319, 334
KspAI GTTAAC 1 cut(s) 436
Kzo9I GATC 1 cut(s) 161
LmnI GCTCC 2 cut(s) 70, 81
LpnPI CCDG 6 cut(s) 66, 92, 144, 291, 308, 390
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 1 cut(s) 207
MaeI CTAG 1 cut(s) 338
MaeII ACGT 2 cut(s) 358, 438
MaeIII GTNAC 3 cut(s) 34, 139, 367
MalI GATC 1 cut(s) 163
MbiI CCGCTC 2 cut(s) 194, 248
MboI GATC 1 cut(s) 161
MboII GAAGA 2 cut(s) 55, 232
MhlI GDGCHC 1 cut(s) 125
MluCI AATT 1 cut(s) 22
MnlI CCTC 4 cut(s) 113, 221, 327, 391
MseI TTAA 2 cut(s) 393, 435
MslI CAYNNNNRTG 1 cut(s) 287
MspI CCGG 2 cut(s) 53, 131
MwoI GCNNNNNNNGC 3 cut(s) 79, 187, 200
NdeII GATC 1 cut(s) 161
NheI GCTAGC 1 cut(s) 337
NlaIII CATG 2 cut(s) 319, 334
NlaIV GGNNCC 2 cut(s) 232, 328
NmeAIII GCCGAG 1 cut(s) 157
NmuCI GTSAC 3 cut(s) 34, 139, 367
NspI RCATGY 1 cut(s) 319
OliI CACNNNNGTG 1 cut(s) 287
PciI ACATGT 1 cut(s) 315
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 219
PkrI GCNGC 1 cut(s) 75
PscI ACATGT 1 cut(s) 315
Psp124BI GAGCTC 1 cut(s) 125
Psp1406I AACGTT 1 cut(s) 438
PspEI GGTNACC 1 cut(s) 139
PspN4I GGNNCC 2 cut(s) 232, 328
PspPI GGNCC 2 cut(s) 231, 327
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
RseI CAYNNNNRTG 1 cut(s) 287
SacI GAGCTC 1 cut(s) 125
SalI GTCGAC 1 cut(s) 344
SaqAI TTAA 2 cut(s) 393, 435
SatI GCNGC 1 cut(s) 74
Sau3AI GATC 1 cut(s) 161
Sau96I GGNCC 2 cut(s) 231, 327
SduI GDGCHC 1 cut(s) 125
SetI ASST 9 cut(s) 69, 78, 117, 125, 141, 297, 361, 382, 441
SfaNI GCATC 1 cut(s) 207
SinI GGWCC 2 cut(s) 231, 327
SmiMI CAYNNNNRTG 1 cut(s) 287
Sse9I AATT 1 cut(s) 22
SsiI CCGC 3 cut(s) 156, 192, 246
SspMI CTAG 1 cut(s) 338
SstI GAGCTC 1 cut(s) 125
TaaI ACNGT 3 cut(s) 34, 109, 289
TaiI ACGT 2 cut(s) 361, 441
TaqI TCGA 3 cut(s) 125, 160, 345
TasI AATT 1 cut(s) 22
Tru1I TTAA 2 cut(s) 393, 435
Tru9I TTAA 2 cut(s) 393, 435
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 3 cut(s) 34, 139, 367
TseI GCWGC 1 cut(s) 73
Tsp45I GTSAC 3 cut(s) 34, 139, 367
TspDTI ATGAA 1 cut(s) 287
TspRI CASTG 1 cut(s) 39
VpaK11BI GGWCC 2 cut(s) 231, 327
XceI RCATGY 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.