Rh4AG061400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003987
Physical Location & Seq
Reverse (-)
13091219 .. 13092333
1115 bp
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UTR
Exon/CDS
Intron
Rh4AG061400.1

Sequence Viewer

Length: 420 bp
ATGGCGGTGCTCGATCCTCTCTCTCCTCTCTCCCTCTTCAGCCCCCTCGCATCACTGAGTGCCTCGTTAGCGATGAGACTGGAACCATTGTCTTCATTGCTCGCAATGACCAAGGATGTTGGGTTGTGGAATTGTGGGTGTGTTGACATTATGAAGCCTGGTACCACTGTGATTCCACGCAATGCAAAGATTGACATGTTCATCAAAGGCACTATGAGGCTTGCAGTTGATAAATGGGGTCGTGTTGAAGTCACAGAACCAGCCAGCTTTGAAGTTAAGGAGGTGAAAAGTGGAAAAAGTAGCTTAGAGCAGATATCTAGAGTTTACGGAAATCAGGTGCATGCCCATAATGCTTCAATTCATTTATATTCTGTTGAGATGATCTACACAGTTTCACGATATGGTATAAAAGTTCTTTGA

Protein Analysis

139

Amino Acids

15.25

Weight (kDa)

8.95

Isoelectric Point (pI)

16.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 48 - 84 1.5e-14 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 161
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 22
AdeI CACNNNGTG 1 cut(s) 59
AfaI GTAC 1 cut(s) 163
AflIII ACRYGT 1 cut(s) 195
AgsI TTSAA 3 cut(s) 248, 272, 357
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 2 cut(s) 267, 303
AluI AGCT 2 cut(s) 267, 303
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 1 cut(s) 70
AlwI GGATC 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 161
AsuHPI GGTGA 1 cut(s) 295
BaeI ACNNNNGTAYC 2 cut(s) 153, 186
BanI GGYRCC 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 12
BciT130I CCWGG 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 70
BfaI CTAG 1 cut(s) 318
Bme1390I CCNGG 1 cut(s) 159
BmiI GGNNCC 2 cut(s) 84, 163
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 84
Bse3DI GCAATG 3 cut(s) 95, 111, 187
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 1 cut(s) 121
BseMI GCAATG 3 cut(s) 95, 111, 187
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 84
BseRI GAGGAG 1 cut(s) 15
BshNI GGYRCC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 12
BsmAI GTCTC 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 12
Bsp143I GATC 2 cut(s) 13, 381
BspACI CCGC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 48
BspLI GGNNCC 2 cut(s) 84, 163
BspPI GGATC 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 161
BsrDI GCAATG 3 cut(s) 95, 111, 187
BsrI ACTGG 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 2 cut(s) 13, 381
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 2 cut(s) 169, 391
Bst6I CTCTTC 1 cut(s) 41
BstC8I GCNNGC 4 cut(s) 102, 222, 265, 342
BstDEI CTNAG 2 cut(s) 56, 304
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 2 cut(s) 16, 384
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 2 cut(s) 13, 381
BstMWI GCNNNNNNNGC 2 cut(s) 68, 350
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 2 cut(s) 199, 344
BstSCI CCNGG 1 cut(s) 157
BstV2I GAAGAC 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 86
BtsCI GGATG 1 cut(s) 121
BtsIMutI CAGTG 2 cut(s) 53, 165
Cac8I GCNNGC 4 cut(s) 102, 222, 265, 342
Csp6I GTAC 1 cut(s) 162
CviAII CATG 2 cut(s) 196, 341
CviJI RGCY 6 cut(s) 42, 157, 220, 263, 267, 303
CviKI_1 RGCY 6 cut(s) 42, 157, 220, 263, 267, 303
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 2 cut(s) 56, 304
DpnI GATC 2 cut(s) 15, 383
DpnII GATC 2 cut(s) 13, 381
DraIII CACNNNGTG 1 cut(s) 59
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 111
Eco32I GATATC 1 cut(s) 315
Eco57I CTGAAG 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 157
EcoRV GATATC 1 cut(s) 315
EcoT14I CCWWGG 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 2 cut(s) 199, 344
FaiI YATR 8 cut(s) 152, 197, 215, 342, 348, 367, 402, 407
FatI CATG 2 cut(s) 195, 340
FokI GGATG 1 cut(s) 128
FspBI CTAG 1 cut(s) 318
Hin1II CATG 2 cut(s) 199, 344
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 1 cut(s) 172
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 2 cut(s) 145, 325
Hpy188III TCNNGA 2 cut(s) 318, 396
Hpy8I GTNNAC 2 cut(s) 145, 325
HpyCH4III ACNGT 2 cut(s) 169, 391
HpyCH4V TGCA 3 cut(s) 185, 224, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 350
HpyF3I CTNAG 2 cut(s) 56, 304
Hsp92II CATG 2 cut(s) 199, 344
KpnI GGTACC 1 cut(s) 165
Kzo9I GATC 2 cut(s) 13, 381
LpnPI CCDG 6 cut(s) 65, 144, 171, 273, 277, 320
LweI GCATC 1 cut(s) 59
MaeI CTAG 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 250
MalI GATC 2 cut(s) 15, 383
MboI GATC 2 cut(s) 13, 381
MboII GAAGA 2 cut(s) 28, 84
MhlI GDGCHC 1 cut(s) 12
MluCI AATT 2 cut(s) 130, 357
MnlI CCTC 7 cut(s) 27, 36, 44, 56, 73, 210, 274
MseI TTAA 1 cut(s) 276
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 68, 350
NdeII GATC 2 cut(s) 13, 381
NlaIII CATG 2 cut(s) 199, 344
NlaIV GGNNCC 2 cut(s) 84, 163
NmuCI GTSAC 1 cut(s) 250
NspI RCATGY 2 cut(s) 199, 344
PaeI GCATGC 1 cut(s) 344
PciI ACATGT 1 cut(s) 195
PfeI GAWTC 1 cut(s) 172
PscI ACATGT 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 2 cut(s) 84, 163
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
SaqAI TTAA 1 cut(s) 276
Sau3AI GATC 2 cut(s) 13, 381
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 1 cut(s) 12
SetI ASST 4 cut(s) 269, 285, 305, 339
SfaNI GCATC 1 cut(s) 59
SphI GCATGC 1 cut(s) 344
Sse9I AATT 2 cut(s) 130, 357
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 318
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 169, 391
TaqI TCGA 1 cut(s) 12
TasI AATT 2 cut(s) 130, 357
TfiI GAWTC 1 cut(s) 172
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 2 cut(s) 60, 172
TseFI GTSAC 1 cut(s) 250
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 4 cut(s) 84, 167, 190, 350
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 2 cut(s) 60, 172
XbaI TCTAGA 1 cut(s) 317
XceI RCATGY 2 cut(s) 199, 344
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.