Rh5AG420800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003987
Physical Location & Seq
Reverse (-)
72609314 .. 72611101
1788 bp
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UTR
Exon/CDS
Intron
Rh5AG420800.1

Sequence Viewer

Length: 291 bp
ATGAGACTGGAACCATTGTCTTCACTGCTCGCAATGACCAAGCCTAGTACCACTGTGATTCCACGCAATACAAAGATTGACATGTTCATCAAAGGCACTATGAGGCTTGCAGTTGATAAATGGGGTCGTGTTGAAGTCACAGAACCAGCCAGCTTTGAAGTTAAGGAGGTGAAAAGCGGAAAAAGTAGCTTAGAGCAAATATCTAGAGTTTACGGAAATCAGGTAATATCTTTGTCTAGTTTTAGGTGCATGCCCATAATGCTTCAATTCATTTATATTCTGTTGAGATGA

Protein Analysis

96

Amino Acids

10.94

Weight (kDa)

9.91

Isoelectric Point (pI)

27.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 12 - 46 2.9e-11 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 177
AfaI GTAC 1 cut(s) 49
AflIII ACRYGT 1 cut(s) 81
AgsI TTSAA 3 cut(s) 134, 158, 266
AluBI AGCT 2 cut(s) 153, 189
AluI AGCT 2 cut(s) 153, 189
AsuHPI GGTGA 1 cut(s) 181
BbsI GAAGAC 1 cut(s) 12
BfaI CTAG 3 cut(s) 45, 204, 237
BmiI GGNNCC 1 cut(s) 12
BpiI GAAGAC 1 cut(s) 12
Bse1I ACTGG 1 cut(s) 12
Bse3DI GCAATG 1 cut(s) 39
BseMI GCAATG 1 cut(s) 39
BseNI ACTGG 1 cut(s) 12
BspACI CCGC 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 1 cut(s) 12
Bst4CI ACNGT 1 cut(s) 55
BstC8I GCNNGC 4 cut(s) 30, 108, 151, 251
BstDEI CTNAG 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 259
BstNSI RCATGY 2 cut(s) 85, 253
BstV2I GAAGAC 1 cut(s) 12
BtsI GCAGTG 1 cut(s) 23
BtsIMutI CAGTG 2 cut(s) 23, 51
Cac8I GCNNGC 4 cut(s) 30, 108, 151, 251
Csp6I GTAC 1 cut(s) 48
CviAII CATG 2 cut(s) 82, 250
CviJI RGCY 5 cut(s) 43, 106, 149, 153, 189
CviKI_1 RGCY 5 cut(s) 43, 106, 149, 153, 189
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 1 cut(s) 190
FaeI CATG 2 cut(s) 85, 253
FaiI YATR 5 cut(s) 83, 101, 251, 257, 276
FatI CATG 2 cut(s) 81, 249
FspBI CTAG 3 cut(s) 45, 204, 237
Hin1II CATG 2 cut(s) 85, 253
HinfI GANTC 1 cut(s) 58
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 211
Hpy188III TCNNGA 1 cut(s) 204
Hpy8I GTNNAC 1 cut(s) 211
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4V TGCA 2 cut(s) 110, 249
HpyF10VI GCNNNNNNNGC 1 cut(s) 259
HpyF3I CTNAG 1 cut(s) 190
Hsp92II CATG 2 cut(s) 85, 253
LpnPI CCDG 3 cut(s) 159, 163, 206
MaeI CTAG 3 cut(s) 45, 204, 237
MaeIII GTNAC 1 cut(s) 136
MboII GAAGA 1 cut(s) 12
MluCI AATT 1 cut(s) 266
MnlI CCTC 2 cut(s) 96, 160
MseI TTAA 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 259
NlaIII CATG 2 cut(s) 85, 253
NlaIV GGNNCC 1 cut(s) 12
NmuCI GTSAC 1 cut(s) 136
NspI RCATGY 2 cut(s) 85, 253
PaeI GCATGC 1 cut(s) 253
PciI ACATGT 1 cut(s) 81
PfeI GAWTC 1 cut(s) 58
PscI ACATGT 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 12
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 162
SetI ASST 5 cut(s) 155, 171, 191, 225, 248
SphI GCATGC 1 cut(s) 253
Sse9I AATT 1 cut(s) 266
SsiI CCGC 1 cut(s) 177
SspMI CTAG 3 cut(s) 45, 204, 237
TaaI ACNGT 1 cut(s) 55
TasI AATT 1 cut(s) 266
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 2 cut(s) 30, 58
TseFI GTSAC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 2 cut(s) 76, 259
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 2 cut(s) 30, 58
XbaI TCTAGA 1 cut(s) 203
XceI RCATGY 2 cut(s) 85, 253
XspI CTAG 3 cut(s) 45, 204, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.