RchiOBHm_Chr2g0127851

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003987
Physical Location & Seq
Reverse (-)
42679946 .. 42681469
1524 bp
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UTR
Exon/CDS
Intron
PRQ49968

Sequence Viewer

Length: 471 bp
ATGGCAACGGCGACAGCGAACGCGTCAGGGGCGACGGTGACGGCGAAGAGAAAGCCGGTGTTCATAAAGGTGGAGGAGATTCAGCCAGGCACGAGCGGCCACACCCTCACCGTCAAGGTCGTCAGCTCCAATCCGGTCAAGGTCACCTCCAAGAACGCCGGCGCCCGATCCTCCCTCTCCTCTCGCCCTCTTCAGCCCCCTCGGATCGCTGAGTGCCTCGTCGGCGATGAGACTGGCACCATTGTCTTCACTGCTCGCAATGACCAAGTTGACATTATGACGCCCGGCACCACTGTGATCCTGCGCAATGCAAAGATTGACATGTTCAAGGGGACTATGAGGCTTGCAGTTGATAAATGGGGTCGTGTTGAAGTTACAGAACCGGCCAGCTTTGAAGTCAAGGAGGCCAACAACCTTTCTCTTGTCGAGTATGAATTAGTTAGTGTTGCGGAAACAGATCAGAAAAGCTAA

Protein Analysis

156

Amino Acids

16.7

Weight (kDa)

8.76

Isoelectric Point (pI)

27.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 27 - 125 4.8e-46 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 305
AccB1I GGYRCC 3 cut(s) 161, 236, 287
AccBSI CCGCTC 1 cut(s) 96
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 96, 449
AclWI GGATC 3 cut(s) 162, 212, 292
AcoI YGGCCR 2 cut(s) 97, 384
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 2 cut(s) 162, 281
AflIII ACRYGT 2 cut(s) 21, 321
AgsI TTSAA 3 cut(s) 328, 371, 395
AjnI CCWGG 1 cut(s) 85
AleI CACNNNNGTG 1 cut(s) 293
AluBI AGCT 3 cut(s) 126, 390, 468
AluI AGCT 3 cut(s) 126, 390, 468
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 3 cut(s) 162, 212, 292
AoxI GGCC 3 cut(s) 97, 384, 405
AspLEI GCGC 2 cut(s) 164, 306
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 3 cut(s) 49, 100, 136
BanI GGYRCC 3 cut(s) 161, 236, 287
BauI CACGAG 1 cut(s) 91
BbsI GAAGAC 1 cut(s) 238
BceAI ACGGC 2 cut(s) 24, 57
BciT130I CCWGG 1 cut(s) 87
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 224
BfoI RGCGCY 1 cut(s) 165
BglI GCCNNNNNGGC 1 cut(s) 222
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
Bme1390I CCNGG 2 cut(s) 87, 285
BmiI GGNNCC 3 cut(s) 163, 238, 289
BmrFI CCNGG 2 cut(s) 87, 285
BpiI GAAGAC 1 cut(s) 238
BpuMI CCSGG 1 cut(s) 285
BsaHI GRCGYC 2 cut(s) 162, 281
BsaJI CCNNGG 1 cut(s) 200
BsaWI WCCGGW 1 cut(s) 133
Bse118I RCCGGY 3 cut(s) 55, 158, 382
Bse1I ACTGG 1 cut(s) 238
Bse3DI GCAATG 2 cut(s) 265, 313
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 200
BseMI GCAATG 2 cut(s) 265, 313
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 2 cut(s) 89, 169
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 99, 386, 407
BshNI GGYRCC 3 cut(s) 161, 236, 287
BsiSI CCGG 5 cut(s) 56, 134, 159, 285, 383
BslFI GGGAC 1 cut(s) 346
BsmAI GTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 346
BsnI GGCC 3 cut(s) 99, 386, 407
Bsp143I GATC 4 cut(s) 167, 204, 297, 457
BspACI CCGC 2 cut(s) 96, 449
BspANI GGCC 3 cut(s) 99, 386, 407
BspCNI CTCAG 1 cut(s) 202
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 163, 238, 289
BspPI GGATC 3 cut(s) 162, 212, 292
BspT107I GGYRCC 3 cut(s) 161, 236, 287
BsrBI CCGCTC 1 cut(s) 96
BsrDI GCAATG 2 cut(s) 265, 313
BsrFI RCCGGY 3 cut(s) 55, 158, 382
BsrI ACTGG 1 cut(s) 238
BssAI RCCGGY 3 cut(s) 55, 158, 382
BssECI CCNNGG 1 cut(s) 200
BssMI GATC 4 cut(s) 167, 204, 297, 457
BssNI GRCGYC 2 cut(s) 162, 281
BssSI CACGAG 1 cut(s) 91
Bst2BI CACGAG 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 3 cut(s) 37, 112, 295
Bst6I CTCTTC 2 cut(s) 41, 195
BstACI GRCGYC 2 cut(s) 162, 281
BstC8I GCNNGC 4 cut(s) 160, 256, 345, 388
BstDEI CTNAG 1 cut(s) 210
BstEII GGTNACC 1 cut(s) 142
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 164, 306
BstKTI GATC 4 cut(s) 170, 207, 300, 460
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 4 cut(s) 167, 204, 297, 457
BstMWI GCNNNNNNNGC 3 cut(s) 29, 96, 222
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 325
BstPI GGTNACC 1 cut(s) 142
BstSCI CCNGG 2 cut(s) 85, 283
BstUI CGCG 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 238
BsuRI GGCC 3 cut(s) 99, 386, 407
BtgZI GCGATG 1 cut(s) 240
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 2 cut(s) 249, 291
Cac8I GCNNGC 4 cut(s) 160, 256, 345, 388
CfoI GCGC 2 cut(s) 164, 306
Cfr10I RCCGGY 3 cut(s) 55, 158, 382
CseI GACGC 2 cut(s) 12, 289
CviAII CATG 1 cut(s) 322
DdeI CTNAG 1 cut(s) 210
DinI GGCGCC 1 cut(s) 163
DpnI GATC 4 cut(s) 169, 206, 299, 459
DpnII GATC 4 cut(s) 167, 204, 297, 457
EaeI YGGCCR 2 cut(s) 97, 384
Eam1104I CTCTTC 2 cut(s) 41, 195
EarI CTCTTC 2 cut(s) 41, 195
Eco57I CTGAAG 1 cut(s) 176
Eco91I GGTNACC 1 cut(s) 142
EcoO65I GGTNACC 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 85
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
FaeI CATG 1 cut(s) 325
FaiI YATR 5 cut(s) 65, 278, 323, 338, 432
FaqI GGGAC 1 cut(s) 346
FatI CATG 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 97
Fsp4HI GCNGC 1 cut(s) 97
FspI TGCGCA 1 cut(s) 305
GlaI GCGC 2 cut(s) 163, 305
GluI GCNGC 1 cut(s) 97
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 3 cut(s) 99, 386, 407
HapII CCGG 5 cut(s) 56, 134, 159, 285, 383
HgaI GACGC 2 cut(s) 12, 289
HhaI GCGC 2 cut(s) 164, 306
Hin1I GRCGYC 2 cut(s) 162, 281
Hin1II CATG 1 cut(s) 325
Hin6I GCGC 2 cut(s) 162, 304
HinP1I GCGC 2 cut(s) 162, 304
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 1 cut(s) 79
HpaII CCGG 5 cut(s) 56, 134, 159, 285, 383
HphI GGTGA 3 cut(s) 49, 100, 136
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 2 cut(s) 204, 462
Hpy8I GTNNAC 1 cut(s) 271
Hpy99I CGWCG 2 cut(s) 37, 224
HpyCH4III ACNGT 3 cut(s) 37, 112, 295
HpyCH4V TGCA 2 cut(s) 311, 347
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 96, 222
HpyF3I CTNAG 1 cut(s) 210
Hsp92I GRCGYC 2 cut(s) 162, 281
Hsp92II CATG 1 cut(s) 325
HspAI GCGC 2 cut(s) 162, 304
KasI GGCGCC 1 cut(s) 161
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
Kzo9I GATC 4 cut(s) 167, 204, 297, 457
LmnI GCTCC 1 cut(s) 131
MaeIII GTNAC 3 cut(s) 37, 142, 373
MalI GATC 4 cut(s) 169, 206, 299, 459
MbiI CCGCTC 1 cut(s) 96
MboI GATC 4 cut(s) 167, 204, 297, 457
MboII GAAGA 3 cut(s) 58, 182, 238
MluCI AATT 1 cut(s) 434
MluI ACGCGT 1 cut(s) 21
Mly113I GGCGCC 1 cut(s) 162
MreI CGCCGGCG 1 cut(s) 158
MroNI GCCGGC 1 cut(s) 158
MslI CAYNNNNRTG 2 cut(s) 68, 293
MspI CCGG 5 cut(s) 56, 134, 159, 285, 383
MspR9I CCNGG 2 cut(s) 87, 285
MvaI CCWGG 1 cut(s) 87
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 29, 96, 222
NaeI GCCGGC 1 cut(s) 160
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 1 cut(s) 285
NdeII GATC 4 cut(s) 167, 204, 297, 457
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 3 cut(s) 163, 238, 289
NmuCI GTSAC 2 cut(s) 37, 142
NsbI TGCGCA 1 cut(s) 305
NspI RCATGY 1 cut(s) 325
OliI CACNNNNGTG 1 cut(s) 293
PciI ACATGT 1 cut(s) 321
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 41
PdiI GCCGGC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 1 cut(s) 98
PluTI GGCGCC 1 cut(s) 165
PscI ACATGT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 85
PspEI GGTNACC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 3 cut(s) 163, 238, 289
RseI CAYNNNNRTG 2 cut(s) 68, 293
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 4 cut(s) 167, 204, 297, 457
ScrFI CCNGG 2 cut(s) 87, 285
SetI ASST 8 cut(s) 72, 120, 128, 144, 149, 392, 417, 470
SfoI GGCGCC 1 cut(s) 163
SgrAI CRCCGGYG 1 cut(s) 158
SmiMI CAYNNNNRTG 2 cut(s) 68, 293
Sse9I AATT 1 cut(s) 434
SsiI CCGC 2 cut(s) 96, 449
SspDI GGCGCC 1 cut(s) 161
StyD4I CCNGG 2 cut(s) 85, 283
TaaI ACNGT 3 cut(s) 37, 112, 295
TaqI TCGA 1 cut(s) 426
TasI AATT 1 cut(s) 434
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 1 cut(s) 79
TscAI CASTG 2 cut(s) 256, 298
TseFI GTSAC 2 cut(s) 37, 142
Tsp45I GTSAC 2 cut(s) 37, 142
TspDTI ATGAA 2 cut(s) 52, 447
TspRI CASTG 2 cut(s) 256, 298
XceI RCATGY 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.