RchiOBHm_Chr2g0127301

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003987
Physical Location & Seq
Forward (+)
42156449 .. 42158347
1899 bp
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UTR
Exon/CDS
Intron
PRQ49918

Sequence Viewer

Length: 474 bp
ATGGCAACGGCGACAGCGAACGCGTCAGGGGCGACGGTGACGGCGAAGAGAAAGCCGGTGTTCATAAAGGTGGAGGAGATTCAGCCAGGCACGAGCGGCCACACCCTCACCGTCAAGGTCGTCAGCTCCAATCCGGTCAAGGTCACCTCCAAGAACGCCGGCGCCCGATCCTCCCTCTCCTCTCGCCCTCTTCAGCCCCTCGGATCGCTGAGTGCCTCGTCGGCGATGAGACTGGCACCATTGTCTTCACTGCTCGCAATGACCAAGATCTTTGACATTATGACGCCCGGCACCACTGTGATCCTGCGCAATGCAAAGATTGACATGTTCAAGGGGACTATGAGGCTTGCAGTTGATAAATGGGGTCGTGTTGAAGTTACAGAACCGGCCAGCTTTGAAGTCAAGGAGGCCAACAACCTTTCTCTTGTCGAGTATGAATTAGTTAGTGTTGCGGAAACAGATCAGAAAAGCTAA

Protein Analysis

157

Amino Acids

16.67

Weight (kDa)

9.79

Isoelectric Point (pI)

28.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 27 - 126 1.2e-26 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 308
AccB1I GGYRCC 3 cut(s) 161, 235, 290
AccBSI CCGCTC 1 cut(s) 96
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 96, 452
AclWI GGATC 3 cut(s) 162, 211, 295
AcoI YGGCCR 2 cut(s) 97, 387
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 2 cut(s) 162, 284
AflIII ACRYGT 2 cut(s) 21, 324
AgsI TTSAA 3 cut(s) 331, 374, 398
AjnI CCWGG 1 cut(s) 85
AleI CACNNNNGTG 1 cut(s) 296
AluBI AGCT 3 cut(s) 126, 393, 471
AluI AGCT 3 cut(s) 126, 393, 471
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 3 cut(s) 162, 211, 295
AoxI GGCC 3 cut(s) 97, 387, 408
AspLEI GCGC 2 cut(s) 164, 309
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 3 cut(s) 49, 100, 136
BanI GGYRCC 3 cut(s) 161, 235, 290
BauI CACGAG 1 cut(s) 91
BbsI GAAGAC 1 cut(s) 237
BceAI ACGGC 2 cut(s) 24, 57
BciT130I CCWGG 1 cut(s) 87
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 1 cut(s) 223
BfoI RGCGCY 1 cut(s) 165
BglI GCCNNNNNGGC 1 cut(s) 221
BglII AGATCT 1 cut(s) 267
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
Bme1390I CCNGG 2 cut(s) 87, 288
BmiI GGNNCC 3 cut(s) 163, 237, 292
BmrFI CCNGG 2 cut(s) 87, 288
BpiI GAAGAC 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 288
BsaHI GRCGYC 2 cut(s) 162, 284
BsaJI CCNNGG 1 cut(s) 199
BsaWI WCCGGW 1 cut(s) 133
Bse118I RCCGGY 3 cut(s) 55, 158, 385
Bse1I ACTGG 1 cut(s) 237
Bse3DI GCAATG 2 cut(s) 264, 316
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 199
BseMI GCAATG 2 cut(s) 264, 316
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 1 cut(s) 237
BseRI GAGGAG 2 cut(s) 89, 169
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 99, 389, 410
BshNI GGYRCC 3 cut(s) 161, 235, 290
BsiSI CCGG 5 cut(s) 56, 134, 159, 288, 386
BslFI GGGAC 1 cut(s) 349
BsmAI GTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 349
BsnI GGCC 3 cut(s) 99, 389, 410
Bsp143I GATC 5 cut(s) 167, 203, 267, 300, 460
BspACI CCGC 2 cut(s) 96, 452
BspANI GGCC 3 cut(s) 99, 389, 410
BspCNI CTCAG 1 cut(s) 201
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 163, 237, 292
BspPI GGATC 3 cut(s) 162, 211, 295
BspT107I GGYRCC 3 cut(s) 161, 235, 290
BsrBI CCGCTC 1 cut(s) 96
BsrDI GCAATG 2 cut(s) 264, 316
BsrFI RCCGGY 3 cut(s) 55, 158, 385
BsrI ACTGG 1 cut(s) 237
BssAI RCCGGY 3 cut(s) 55, 158, 385
BssECI CCNNGG 1 cut(s) 199
BssMI GATC 5 cut(s) 167, 203, 267, 300, 460
BssNI GRCGYC 2 cut(s) 162, 284
BssSI CACGAG 1 cut(s) 91
Bst2BI CACGAG 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 3 cut(s) 37, 112, 298
Bst6I CTCTTC 2 cut(s) 41, 195
BstACI GRCGYC 2 cut(s) 162, 284
BstC8I GCNNGC 4 cut(s) 160, 255, 348, 391
BstDEI CTNAG 1 cut(s) 209
BstEII GGTNACC 1 cut(s) 142
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 164, 309
BstKTI GATC 5 cut(s) 170, 206, 270, 303, 463
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 5 cut(s) 167, 203, 267, 300, 460
BstMWI GCNNNNNNNGC 3 cut(s) 29, 96, 221
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 328
BstPI GGTNACC 1 cut(s) 142
BstSCI CCNGG 2 cut(s) 85, 286
BstUI CGCG 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 1 cut(s) 267
BstYI RGATCY 1 cut(s) 267
BsuRI GGCC 3 cut(s) 99, 389, 410
BtgZI GCGATG 1 cut(s) 239
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 2 cut(s) 248, 294
Cac8I GCNNGC 4 cut(s) 160, 255, 348, 391
CfoI GCGC 2 cut(s) 164, 309
Cfr10I RCCGGY 3 cut(s) 55, 158, 385
CseI GACGC 2 cut(s) 12, 292
CviAII CATG 1 cut(s) 325
DdeI CTNAG 1 cut(s) 209
DinI GGCGCC 1 cut(s) 163
DpnI GATC 5 cut(s) 169, 205, 269, 302, 462
DpnII GATC 5 cut(s) 167, 203, 267, 300, 460
EaeI YGGCCR 2 cut(s) 97, 387
Eam1104I CTCTTC 2 cut(s) 41, 195
EarI CTCTTC 2 cut(s) 41, 195
Eco57I CTGAAG 1 cut(s) 176
Eco91I GGTNACC 1 cut(s) 142
EcoO65I GGTNACC 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 85
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
FaeI CATG 1 cut(s) 328
FaiI YATR 5 cut(s) 65, 281, 326, 341, 435
FaqI GGGAC 1 cut(s) 349
FatI CATG 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 97
Fsp4HI GCNGC 1 cut(s) 97
FspI TGCGCA 1 cut(s) 308
GlaI GCGC 2 cut(s) 163, 308
GluI GCNGC 1 cut(s) 97
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 3 cut(s) 99, 389, 410
HapII CCGG 5 cut(s) 56, 134, 159, 288, 386
HgaI GACGC 2 cut(s) 12, 292
HhaI GCGC 2 cut(s) 164, 309
Hin1I GRCGYC 2 cut(s) 162, 284
Hin1II CATG 1 cut(s) 328
Hin6I GCGC 2 cut(s) 162, 307
HinP1I GCGC 2 cut(s) 162, 307
HinfI GANTC 1 cut(s) 79
HpaII CCGG 5 cut(s) 56, 134, 159, 288, 386
HphI GGTGA 3 cut(s) 49, 100, 136
Hpy188I TCNGA 2 cut(s) 203, 465
Hpy99I CGWCG 2 cut(s) 37, 223
HpyCH4III ACNGT 3 cut(s) 37, 112, 298
HpyCH4V TGCA 2 cut(s) 314, 350
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 96, 221
HpyF3I CTNAG 1 cut(s) 209
Hsp92I GRCGYC 2 cut(s) 162, 284
Hsp92II CATG 1 cut(s) 328
HspAI GCGC 2 cut(s) 162, 307
KasI GGCGCC 1 cut(s) 161
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
Kzo9I GATC 5 cut(s) 167, 203, 267, 300, 460
LmnI GCTCC 1 cut(s) 131
MaeIII GTNAC 3 cut(s) 37, 142, 376
MalI GATC 5 cut(s) 169, 205, 269, 302, 462
MbiI CCGCTC 1 cut(s) 96
MboI GATC 5 cut(s) 167, 203, 267, 300, 460
MboII GAAGA 3 cut(s) 58, 182, 237
MflI RGATCY 1 cut(s) 267
MluCI AATT 1 cut(s) 437
MluI ACGCGT 1 cut(s) 21
Mly113I GGCGCC 1 cut(s) 162
MreI CGCCGGCG 1 cut(s) 158
MroNI GCCGGC 1 cut(s) 158
MslI CAYNNNNRTG 2 cut(s) 68, 296
MspI CCGG 5 cut(s) 56, 134, 159, 288, 386
MspR9I CCNGG 2 cut(s) 87, 288
MvaI CCWGG 1 cut(s) 87
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 29, 96, 221
NaeI GCCGGC 1 cut(s) 160
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 1 cut(s) 288
NdeII GATC 5 cut(s) 167, 203, 267, 300, 460
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 1 cut(s) 328
NlaIV GGNNCC 3 cut(s) 163, 237, 292
NmuCI GTSAC 2 cut(s) 37, 142
NsbI TGCGCA 1 cut(s) 308
NspI RCATGY 1 cut(s) 328
OliI CACNNNNGTG 1 cut(s) 296
PciI ACATGT 1 cut(s) 324
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 41
PdiI GCCGGC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 1 cut(s) 98
PluTI GGCGCC 1 cut(s) 165
PscI ACATGT 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 85
PspEI GGTNACC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 3 cut(s) 163, 237, 292
PsuI RGATCY 1 cut(s) 267
RseI CAYNNNNRTG 2 cut(s) 68, 296
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 5 cut(s) 167, 203, 267, 300, 460
ScrFI CCNGG 2 cut(s) 87, 288
SetI ASST 8 cut(s) 72, 120, 128, 144, 149, 395, 420, 473
SfoI GGCGCC 1 cut(s) 163
SgrAI CRCCGGYG 1 cut(s) 158
SmiMI CAYNNNNRTG 2 cut(s) 68, 296
Sse9I AATT 1 cut(s) 437
SsiI CCGC 2 cut(s) 96, 452
SspDI GGCGCC 1 cut(s) 161
StyD4I CCNGG 2 cut(s) 85, 286
TaaI ACNGT 3 cut(s) 37, 112, 298
TaqI TCGA 1 cut(s) 429
TasI AATT 1 cut(s) 437
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 1 cut(s) 79
TscAI CASTG 2 cut(s) 255, 301
TseFI GTSAC 2 cut(s) 37, 142
Tsp45I GTSAC 2 cut(s) 37, 142
TspDTI ATGAA 2 cut(s) 52, 450
TspRI CASTG 2 cut(s) 255, 301
XceI RCATGY 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.