AT4G30600

Signal recognition particle receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002654
Physical Location & Seq
Reverse (-)
14937840 .. 14941208
3369 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30600.2

Sequence Viewer

Length: 1905 bp
ATGTTAGAACAGCTTTTAATCTTTACCCGAGGAGGATTAATCCTTTGGACATGCAAAGAGATTGGTAATGCATTAAAAGGATCACCTATCGATACTTTGATCCGGTCCTGTCTCCTTGAGGAACGATCTGGTGCTGTTTCGTTTAACTACGATGCTCCTGGTGCTGCCTACACTCTCAAGTGGACGTTTCACAATGATCTTGGACTTGTCTTTGTCGCTGTTTATCAGCGGATTCTCCATTTGCTTTATGTGGATGATTTGCTTTCTATGGTCAAGCAAAGCTTCTCTGAGGTCTATGATCCTAAACGGATGGCTTATGATGATTTCGATGAGACTTTTAGGCAGCTTAGGATTGAAGCTGAAGCTCGTGCTGAGGAATTGAGGAAGACTAAGCAAGTGGGGAAGCCTGTGACCAGTGTTAAGAAGCAAGGGCAGGTTTCAAAACCTGGACTTGAAGGAGGAAACAAAAGGGTTAGTAGTGAAGGTGGTTCGAAGAAAGATGATGGCGATGGTGGTAATAAGGCCAAAGTTAGTACCTTGACTAATGGTCATTCCAATGGGAATCATCAAATGGAAGATGATTCTCAGGAGACTAATGATCTTGCTAACGGGAAAGAAAACACGAGTTCTAATGTTGCTGTTGATTTAAGTAAACTTCAGAAACTTAGAAGTAAAGGAGTCAGAGGTAGAGGAGGTGTAAGGAAAACTGACAGTATAGGTAATAAGAGTTCCAAGGTAGCGGAACCAGCCAAAAAGGCGACAAAGAAAAACAGGGTTTGGGATGATGCGGCTCCCAAACAATCGAAATTGGACTTCACGGATTCCATCGATGAAAATGGGAACAATGATCATGTAGATATTGTGGCTGCTGACCAAGGAGAAAGTATGATGGACAAGGAAGAGGTTTTCAGCAGTGATAGTGAAAGTGAAGATGATGACGAACCAGGAAGTGATGAGAAGCCTGCTCAGGCTAAGAAAAAGGGATGGTTTTCTTCTGTTTTCCAGAGTATTACTGGGAAAGCGAATCTTGAAAGGACAGACCTTGGACCGGCGTTGAAAGCTCTGAAGGAACGGCTCATGACCAAGAATGTGGCAGAAGAGATTGCTGAGAAGCTTTGCGAATCAGTGGAAGCTAGTCTTGAAGGAAAGAAATTGTCATCTTTCACCAGGATCTCTTCAACCGTTCAGGCAGCGATGGAGGATGCTCTGGTTCGTATATTGACTCCAAGACGCTCCATTGATATATTAAGAGATGTTCATGCTGCCAAAGAACAGAGGAAACCTTATGTGGTTGTGTTTGTTGGAGTCAATGGAGTTGGGAAATCCACCAATCTCGCTAAAGTGGCGTATTGGCTTCAGCAGCATAAGGTCAGTGTAATGATGGCTGCTTGTGACACTTTCCGTTCTGGAGCTGTTGAGCAGTTACGGACTCATGCTCGTAGGTTACAGATACCGATATTTGAAAAGGGTTATGAAAAGGATCCAGCAGTAGTTGCTAAAGAAGCCATACAAGAAGCAACTCGAAATGGATCCGATGTTGTTCTTGTTGACACAGCTGGTCGGATGCAGGATAATGAACCTTTGATGAGAGCACTCTCAAAGCTCATCAACCTTAATCAGCCGGACTTGGTCTTGTTTGTTGGTGAAGCTCTTGTTGGAAACGATGCAGTAGACCAGCTCTCGAAGTTCAATCAGAAACTTTCGGATCTCTCGACTTCTGGGAACCCAAGACTGATCGATGGAATCTTACTTACAAAGTTTGATACCATTGACGACAAGGTCGGAGCAGCGTTGTCTATGGTTTACATATCGGGATCACCGGTTATGTTCGTGGGTTGTGGCCAGTCTTACACTGACCTGAAGAAGCTTAATGTCAAAGCCATAGTCAAGACACTTCTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
SRP-alpha_N SRP54 SRP54_N

Protein Analysis

634

Amino Acids

69.57

Weight (kDa)

7.1

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP-alpha_N PF04086 28 - 310 2.9e-65 Signal recognition particle, alpha subunit, N-terminal
SRP54_N PF02881 330 - 398 3.5e-09 SRP54-type protein, helical bundle domain
AAA_22 PF13401 427 - 543 4.8e-06 AAA domain
SRP54 PF00448 429 - 633 5.5e-57 SRP54-type protein, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1778
Acc36I ACCTGC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 1671
AciI CCGC 3 cut(s) 229, 740, 788
AcoI YGGCCR 1 cut(s) 1840
AcuI CTGAAG 5 cut(s) 381, 641, 1085, 1340, 1880
AfaI GTAC 1 cut(s) 535
AfiI CCNNNNNNNGG 1 cut(s) 1048
AgeI ACCGGT 1 cut(s) 1819
AgsI TTSAA 9 cut(s) 356, 441, 455, 1031, 1057, 1142, 1179, 1463, 1690
AhdI GACNNNNNGTC 1 cut(s) 546
AjnI CCWGG 4 cut(s) 157, 445, 943, 1166
AjuI GAANNNNNNNTTGG 2 cut(s) 1638, 1670
Alw21I GWGCWC 1 cut(s) 1594
Alw26I GTCTC 3 cut(s) 116, 326, 584
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 2 cut(s) 522, 1840
ApeKI GCWGC 8 cut(s) 164, 343, 866, 1190, 1262, 1360, 1385, 1787
ArsI GACNNNNNNTTYG 2 cut(s) 1869, 1901
AseI ATTAAT 1 cut(s) 38
AsiGI ACCGGT 1 cut(s) 1819
AspS9I GGNCC 2 cut(s) 105, 1046
AsuHPI GGTGA 4 cut(s) 75, 1156, 1655, 1809
AsuII TTCGAA 1 cut(s) 491
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 2 cut(s) 105, 1046
BalI TGGCCA 1 cut(s) 1842
BamHI GGATCC 2 cut(s) 1480, 1529
BauI CACGAG 2 cut(s) 366, 622
BbsI GAAGAC 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 1594
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 8 cut(s) 151, 355, 853, 1202, 1249, 1372, 1372, 1799
BccI CCATC 9 cut(s) 304, 497, 503, 833, 883, 978, 1189, 1375, 1733
BceAI ACGGC 1 cut(s) 1088
BciT130I CCWGG 4 cut(s) 159, 447, 945, 1168
BclI TGATCA 1 cut(s) 847
BcoDI GTCTC 3 cut(s) 116, 326, 584
BfaI CTAG 1 cut(s) 1134
BfuAI ACCTGC 1 cut(s) 424
BglI GCCNNNNNGGC 1 cut(s) 755
BisI GCNGC 9 cut(s) 165, 344, 789, 867, 1191, 1263, 1361, 1386, 1788
BlsI GCNGC 9 cut(s) 166, 345, 790, 868, 1192, 1264, 1362, 1387, 1789
Bme1390I CCNGG 4 cut(s) 159, 447, 945, 1168
Bme18I GGWCC 2 cut(s) 105, 1046
BmeRI GACNNNNNGTC 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 105, 1046
BmiI GGNNCC 5 cut(s) 744, 792, 1482, 1531, 1724
BmrFI CCNGG 4 cut(s) 159, 447, 945, 1168
BmrI ACTGGG 1 cut(s) 1023
BmsI GCATC 5 cut(s) 142, 775, 1192, 1554, 1654
BmuI ACTGGG 1 cut(s) 1023
BpiI GAAGAC 1 cut(s) 392
BpmI CTGGAG 1 cut(s) 1428
Bpu10I CCTNAGC 3 cut(s) 347, 372, 966
Bpu14I TTCGAA 1 cut(s) 491
BpuEI CTTGAG 3 cut(s) 137, 161, 1883
Bsa29I ATCGAT 3 cut(s) 90, 828, 1737
BsaJI CCNNGG 4 cut(s) 28, 732, 874, 1042
BsaWI WCCGGW 2 cut(s) 102, 1819
Bsc4I CCNNNNNNNGG 1 cut(s) 1048
Bse118I RCCGGY 2 cut(s) 1048, 1819
Bse1I ACTGG 3 cut(s) 414, 1018, 1843
BseBI CCWGG 4 cut(s) 159, 447, 945, 1168
BseCI ATCGAT 3 cut(s) 90, 828, 1737
BseDI CCNNGG 4 cut(s) 28, 732, 874, 1042
BseGI GGATG 6 cut(s) 259, 315, 787, 989, 1207, 1569
BseLI CCNNNNNNNGG 1 cut(s) 1048
BseMII CTCAG 5 cut(s) 279, 363, 599, 980, 1098
BseNI ACTGG 3 cut(s) 414, 1018, 1843
BseRI GAGGAG 2 cut(s) 45, 705
BseXI GCAGC 8 cut(s) 151, 355, 853, 1202, 1249, 1372, 1372, 1799
BshFI GGCC 2 cut(s) 524, 1842
BshTI ACCGGT 1 cut(s) 1819
BshVI ATCGAT 3 cut(s) 90, 828, 1737
BsiHKAI GWGCWC 1 cut(s) 1594
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 4 cut(s) 103, 1049, 1622, 1820
BslI CCNNNNNNNGG 1 cut(s) 1048
BsmAI GTCTC 3 cut(s) 116, 326, 584
BsnI GGCC 2 cut(s) 524, 1842
BsoBI CYCGRG 1 cut(s) 27
Bsp119I TTCGAA 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 1594
BspACI CCGC 3 cut(s) 229, 740, 788
BspANI GGCC 2 cut(s) 524, 1842
BspCNI CTCAG 5 cut(s) 280, 364, 598, 979, 1099
BspDI ATCGAT 3 cut(s) 90, 828, 1737
BspHI TCATGA 1 cut(s) 1077
BspLI GGNNCC 5 cut(s) 744, 792, 1482, 1531, 1724
BspMI ACCTGC 1 cut(s) 424
BspT104I TTCGAA 1 cut(s) 491
BsrFI RCCGGY 2 cut(s) 1048, 1819
BsrI ACTGG 3 cut(s) 414, 1018, 1843
BssAI RCCGGY 2 cut(s) 1048, 1819
BssECI CCNNGG 4 cut(s) 28, 732, 874, 1042
BssSI CACGAG 2 cut(s) 366, 622
BssT1I CCWWGG 3 cut(s) 732, 874, 1042
Bst2BI CACGAG 2 cut(s) 366, 622
Bst2UI CCWGG 4 cut(s) 159, 447, 945, 1168
Bst4CI ACNGT 2 cut(s) 713, 1183
Bst6I CTCTTC 3 cut(s) 894, 1092, 1180
BstAPI GCANNNNNTGC 1 cut(s) 1493
BstBI TTCGAA 1 cut(s) 491
BstC8I GCNNGC 1 cut(s) 963
BstDEI CTNAG 9 cut(s) 288, 347, 372, 390, 585, 665, 966, 972, 1107
BstF5I GGATG 6 cut(s) 259, 315, 787, 989, 1207, 1569
BstMAI GTCTC 3 cut(s) 116, 326, 584
BstMWI GCNNNNNNNGC 8 cut(s) 161, 746, 755, 1058, 1343, 1360, 1493, 1502
BstNI CCWGG 4 cut(s) 159, 447, 945, 1168
BstNSI RCATGY 1 cut(s) 54
BstSCI CCNGG 4 cut(s) 157, 445, 943, 1166
BstV1I GCAGC 8 cut(s) 151, 355, 853, 1202, 1249, 1372, 1372, 1799
BstV2I GAAGAC 1 cut(s) 392
BstX2I RGATCY 4 cut(s) 1170, 1480, 1529, 1705
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstYI RGATCY 4 cut(s) 1170, 1480, 1529, 1705
Bsu15I ATCGAT 3 cut(s) 90, 828, 1737
BsuRI GGCC 2 cut(s) 524, 1842
BsuTUI ATCGAT 3 cut(s) 90, 828, 1737
BtgZI GCGATG 2 cut(s) 522, 1208
BtsCI GGATG 6 cut(s) 259, 315, 787, 989, 1207, 1569
BtsI GCAGTG 1 cut(s) 919
BtsIMutI CAGTG 5 cut(s) 421, 919, 1131, 1378, 1851
BveI ACCTGC 1 cut(s) 424
Cac8I GCNNGC 1 cut(s) 963
CciI TCATGA 1 cut(s) 1077
Cfr10I RCCGGY 2 cut(s) 1048, 1819
Cfr13I GGNCC 2 cut(s) 105, 1046
ClaI ATCGAT 3 cut(s) 90, 828, 1737
CseI GACGC 1 cut(s) 1239
Csp6I GTAC 1 cut(s) 534
CspAI ACCGGT 1 cut(s) 1819
CviAII CATG 5 cut(s) 51, 851, 1078, 1259, 1433
CviQI GTAC 1 cut(s) 534
DdeI CTNAG 9 cut(s) 288, 347, 372, 390, 585, 665, 966, 972, 1107
DrdI GACNNNNNNGTC 1 cut(s) 1778
DriI GACNNNNNGTC 1 cut(s) 546
DseDI GACNNNNNNGTC 1 cut(s) 1778
EaeI YGGCCR 1 cut(s) 1840
Eam1104I CTCTTC 3 cut(s) 894, 1092, 1180
Eam1105I GACNNNNNGTC 1 cut(s) 546
EarI CTCTTC 3 cut(s) 894, 1092, 1180
Eco130I CCWWGG 3 cut(s) 732, 874, 1042
Eco47I GGWCC 2 cut(s) 105, 1046
Eco57I CTGAAG 5 cut(s) 381, 641, 1085, 1340, 1880
Eco88I CYCGRG 1 cut(s) 27
EcoRII CCWGG 4 cut(s) 157, 445, 943, 1166
EcoT14I CCWWGG 3 cut(s) 732, 874, 1042
EcoT22I ATGCAT 1 cut(s) 73
ErhI CCWWGG 3 cut(s) 732, 874, 1042
FaeI CATG 5 cut(s) 54, 854, 1081, 1262, 1436
FalI AAGNNNNNCTT 6 cut(s) 266, 298, 1011, 1043, 1122, 1154
FatI CATG 5 cut(s) 50, 850, 1077, 1258, 1432
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 1 cut(s) 1671
Fnu4HI GCNGC 9 cut(s) 165, 344, 789, 867, 1191, 1263, 1361, 1386, 1788
FokI GGATG 6 cut(s) 266, 322, 794, 996, 1214, 1576
Fsp4HI GCNGC 9 cut(s) 165, 344, 789, 867, 1191, 1263, 1361, 1386, 1788
FspBI CTAG 1 cut(s) 1134
GluI GCNGC 9 cut(s) 165, 344, 789, 867, 1191, 1263, 1361, 1386, 1788
GsuI CTGGAG 1 cut(s) 1428
HaeIII GGCC 2 cut(s) 524, 1842
HapII CCGG 4 cut(s) 103, 1049, 1622, 1820
HgaI GACGC 1 cut(s) 1239
Hin1II CATG 5 cut(s) 54, 854, 1081, 1262, 1436
HincII GTYRAC 1 cut(s) 1549
HindII GTYRAC 1 cut(s) 1549
HindIII AAGCTT 3 cut(s) 280, 1112, 1865
HpaII CCGG 4 cut(s) 103, 1049, 1622, 1820
HphI GGTGA 4 cut(s) 75, 1156, 1655, 1809
Hpy166II GTNNAC 5 cut(s) 183, 653, 1549, 1672, 1804
Hpy188I TCNGA 9 cut(s) 289, 660, 683, 1065, 1534, 1563, 1695, 1705, 1784
Hpy8I GTNNAC 5 cut(s) 183, 653, 1549, 1672, 1804
HpyAV CCTTC 4 cut(s) 449, 476, 1060, 1136
HpyCH4III ACNGT 2 cut(s) 713, 1183
HpyCH4IV ACGT 1 cut(s) 185
HpyCH4V TGCA 4 cut(s) 54, 71, 1567, 1667
HpyF10VI GCNNNNNNNGC 8 cut(s) 161, 746, 755, 1058, 1343, 1360, 1493, 1502
HpyF3I CTNAG 9 cut(s) 288, 347, 372, 390, 585, 665, 966, 972, 1107
HpySE526I ACGT 1 cut(s) 185
Hsp92II CATG 5 cut(s) 54, 854, 1081, 1262, 1436
Ksp22I TGATCA 1 cut(s) 847
LmnI GCTCC 5 cut(s) 160, 796, 1238, 1409, 1784
Lsp1109I GCAGC 8 cut(s) 151, 355, 853, 1202, 1249, 1372, 1372, 1799
LweI GCATC 5 cut(s) 142, 775, 1192, 1554, 1654
MaeI CTAG 1 cut(s) 1134
MaeII ACGT 1 cut(s) 185
MaeIII GTNAC 4 cut(s) 409, 1391, 1422, 1443
MboII GAAGA 9 cut(s) 397, 505, 587, 911, 941, 984, 1109, 1167, 1873
MflI RGATCY 4 cut(s) 1170, 1480, 1529, 1705
MhlI GDGCHC 1 cut(s) 1594
MlsI TGGCCA 1 cut(s) 1842
MluCI AATT 3 cut(s) 377, 806, 1151
MluNI TGGCCA 1 cut(s) 1842
MlyI GAGTC 4 cut(s) 687, 1216, 1314, 1423
MmeI TCCRAC 4 cut(s) 1282, 1541, 1636, 1762
Mox20I TGGCCA 1 cut(s) 1842
Mph1103I ATGCAT 1 cut(s) 73
MscI TGGCCA 1 cut(s) 1842
MseI TTAA 9 cut(s) 17, 38, 74, 144, 420, 647, 1247, 1614, 1869
MslI CAYNNNNRTG 1 cut(s) 555
Msp20I TGGCCA 1 cut(s) 1842
MspA1I CMGCKG 2 cut(s) 229, 1556
MspI CCGG 4 cut(s) 103, 1049, 1622, 1820
MspR9I CCNGG 4 cut(s) 159, 447, 945, 1168
MvaI CCWGG 4 cut(s) 159, 447, 945, 1168
MwoI GCNNNNNNNGC 8 cut(s) 161, 746, 755, 1058, 1343, 1360, 1493, 1502
NlaIII CATG 5 cut(s) 54, 854, 1081, 1262, 1436
NlaIV GGNNCC 5 cut(s) 744, 792, 1482, 1531, 1724
NmuCI GTSAC 2 cut(s) 409, 1391
NsiI ATGCAT 1 cut(s) 73
NspI RCATGY 1 cut(s) 54
NspV TTCGAA 1 cut(s) 491
PagI TCATGA 1 cut(s) 1077
PcsI WCGNNNNNNNCGW 1 cut(s) 1709
PfeI GAWTC 7 cut(s) 232, 562, 581, 821, 1024, 1121, 1743
PflFI GACNNNGTC 2 cut(s) 1628, 1778
PinAI ACCGGT 1 cut(s) 1819
PkrI GCNGC 9 cut(s) 166, 345, 790, 868, 1192, 1264, 1362, 1387, 1789
PleI GAGTC 4 cut(s) 686, 1216, 1313, 1423
PpsI GAGTC 4 cut(s) 686, 1216, 1313, 1423
PshBI ATTAAT 1 cut(s) 38
Psp6I CCWGG 4 cut(s) 157, 445, 943, 1166
PspGI CCWGG 4 cut(s) 157, 445, 943, 1166
PspN4I GGNNCC 5 cut(s) 744, 792, 1482, 1531, 1724
PspPI GGNCC 2 cut(s) 105, 1046
PsrI GAACNNNNNNTAC 2 cut(s) 712, 744
PsuI RGATCY 4 cut(s) 1170, 1480, 1529, 1705
PsyI GACNNNGTC 2 cut(s) 1628, 1778
PvuII CAGCTG 1 cut(s) 1556
RsaI GTAC 1 cut(s) 535
RsaNI GTAC 1 cut(s) 534
RseI CAYNNNNRTG 1 cut(s) 555
SaqAI TTAA 9 cut(s) 17, 38, 74, 144, 420, 647, 1247, 1614, 1869
SatI GCNGC 9 cut(s) 165, 344, 789, 867, 1191, 1263, 1361, 1386, 1788
Sau96I GGNCC 2 cut(s) 105, 1046
SchI GAGTC 4 cut(s) 687, 1216, 1314, 1423
ScrFI CCNGG 4 cut(s) 159, 447, 945, 1168
SduI GDGCHC 1 cut(s) 1594
SfaNI GCATC 5 cut(s) 142, 775, 1192, 1554, 1654
SfuI TTCGAA 1 cut(s) 491
SinI GGWCC 2 cut(s) 105, 1046
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 3 cut(s) 116, 176, 1898
SmoI CTYRAG 3 cut(s) 116, 176, 1898
Sse9I AATT 3 cut(s) 377, 806, 1151
SsiI CCGC 3 cut(s) 229, 740, 788
SspMI CTAG 1 cut(s) 1134
StyD4I CCNGG 4 cut(s) 157, 445, 943, 1166
StyI CCWWGG 3 cut(s) 732, 874, 1042
TaaI ACNGT 2 cut(s) 713, 1183
TaiI ACGT 1 cut(s) 188
TaqI TCGA 9 cut(s) 90, 327, 491, 803, 828, 1522, 1682, 1712, 1737
TasI AATT 3 cut(s) 377, 806, 1151
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 7 cut(s) 232, 562, 581, 821, 1024, 1121, 1743
Tru1I TTAA 9 cut(s) 17, 38, 74, 144, 420, 647, 1247, 1614, 1869
Tru9I TTAA 9 cut(s) 17, 38, 74, 144, 420, 647, 1247, 1614, 1869
TscAI CASTG 5 cut(s) 421, 919, 1131, 1378, 1858
TseFI GTSAC 2 cut(s) 409, 1391
TseI GCWGC 8 cut(s) 164, 343, 866, 1190, 1262, 1360, 1385, 1787
Tsp45I GTSAC 2 cut(s) 409, 1391
TspDTI ATGAA 4 cut(s) 846, 1247, 1488, 1590
TspGWI ACGGA 4 cut(s) 322, 833, 1391, 1441
TspRI CASTG 5 cut(s) 421, 919, 1131, 1378, 1858
Tth111I GACNNNGTC 2 cut(s) 1628, 1778
VpaK11BI GGWCC 2 cut(s) 105, 1046
VspI ATTAAT 1 cut(s) 38
XceI RCATGY 1 cut(s) 54
XcmI CCANNNNNNNNNTGG 1 cut(s) 1010
XmiI GTMKAC 1 cut(s) 1671
XspI CTAG 1 cut(s) 1134
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.