MD02G1006600.v1.1

Signal recognition particle receptor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002654
Physical Location & Seq
Forward (+)
451396 .. 455362
3967 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1006600.v1.1.491

Sequence Viewer

Length: 1866 bp
ATGTTAGAACAGTTACTGATATTTACTAGAGGAGGATTAATTCTCTGGACATGCAAAGAGCTTGGAAATGCTCTTAAGGGATCTCCAATCGACACCTTGATCCGATCGTGTCTTTTGGAGGAACGGTCTGGTTTAGCTTCTTTCGATTACAATGCCCCTGGGGCTGCTTACACGCTCAAATGGACGTTCCACAATGAGCTCGGTCTTGTGTTTGTCGCTGTTTATCAGAAGATCCTCCATCTGTTGTATGTGGATGAACTGCTTTCGATGGTGAAACATGAGTTTTCGGAGATTTATGATCCAAAAAGGACAGTGTATGCTGACTTTGATGAAACTTTTCGACAACTGAAGAAGGAGGCTGAGGCTAGGGCTGAGGAATTGAAGAAGTCGAAGCAGGTGGGTAAGCCTGTGAACAATAAGAAGCAAGGGCAGGTGCAGAAGGGGGGACTTGGAGGAGACAAGAAAAAGAATGAAGGTGGTTTGGCAAGTGACGGTGGGGATGGTGATAATATGAAGGGCCGTAAATTGGAGAATGGGATCTCCAACGGTAATCACGTGGATTTCAAAGAATCTAATGGTACTGCTAACGGAAAAGAAAATACTAGTTCCAATCTTGGGGCTTTTGATGTAAATAAGCTTCAGAAACTTAGATCTAAGGCTGGGAAGAAGACAGAGAAAACAGCTACTGTTGATAGCAAGAGCTCCAAGGCTGAGCCAAAAAAGAAGATAACAAAGAAGAATAGAGTTTGGGATGATAAACCTCCCGAGTCGAAACTGGATTTTACAGATCCTATAGGTGAGAACGGAGACAACAATATTGAGGTCGTGGCAGCAGATCACGGTGACAGTATGATGGACAAAGAAGAGGATTTCAGTAGTGAAAGTGAGGAGGAAGAGGAAGAGGATGTTGGGAAGGAAAGCAAACCTGATAAGCAAAAGAAGGTGTGGTTTTCATCCATGTTCCAGAGTATTGCTGGCAAGGCGAACTTGGAGAGGTCTGACCTGGAACCAGCTTTGAAAGCTCTCAAGGATAGGCTCATGACCAAGAACGTGGCCGAGGAGATAGCTGAGAAGCTTTGTGAATCAGTGGCAGCTAGTCTTGAGGGTAAAAAGCTGGCTTCGTTCACAAGAATATCTTCTACTGTGCAGACTGCAATGGAAGATGCTCTTGTTCGTATTTTAACTCCCAGGCGCTCAATTGATATACTGAGGGATGTGCATGCTGCCAAGGAACAAAGGAAGCCGTATGTCGTCGTTTTTGTTGGTGTCAATGGAGTTGGGAAATCTACCAATTTGGCTAAGGTTGCTTATTGGCTTCAGCAGCATAACGTCAGTGTCATGATGGCTGCCTGCGATACATTTCGATCAGGAGCTGTTGAGCAGCTGCGTACTCATGCACGCAGACTCCAGATCCCTATTTTTGAGAAGGGATACGAGAAAGATCCTGCGGTCGTGGCTAAGGAAGCAATCCAGGAGGCCACACACAATGGTTCTGATGTGGTTCTTGTTGATACGGCTGGTCGTATGCAGGATAATGAGCCATTGATGAGGGCACTGTCCAAGCTTGTCAATCTTAACAACCCAGATCTTGTCTTGTTCGTTGGAGAGGCACTGGTTGGGAATGATGCTGTTGATCAGCTTTCGAAGTTTAATCAGAAATTAGCGGACCTCTCAACTTCCCAAAACCCGAGATTGATAGATGGGATATTGCTCACTAAGTTCGACACTATTGATGATAAGGTTGGGGCTGCGCTGTCCATGGTTTACATATCTGGAGCTCCGGTCATGTTTGTCGGGTGTGGCCAGTCGTACACTGACCTCAAGAAGCTGAATGTGAAATCGATCGTGAAGACGCTCCTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
SRP-alpha_N SRP54 SRP54_N

Protein Analysis

622

Amino Acids

68.54

Weight (kDa)

7.12

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP-alpha_N PF04086 28 - 298 3.4e-63 Signal recognition particle, alpha subunit, N-terminal
SRP54_N PF02881 317 - 385 6.5e-09 SRP54-type protein, helical bundle domain
AAA_22 PF13401 414 - 531 7.7e-07 AAA domain
SRP54 PF00448 416 - 620 5.2e-57 SRP54-type protein, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 385, 421
Acc36I ACCTGC 2 cut(s) 385, 421
AciI CCGC 2 cut(s) 1448, 1664
AclWI GGATC 8 cut(s) 88, 94, 226, 293, 545, 782, 1405, 1436
AcoI YGGCCR 2 cut(s) 1053, 1801
AcuI CTGAAG 3 cut(s) 368, 623, 1301
AcvI CACGTG 1 cut(s) 556
AfaI GTAC 3 cut(s) 580, 1390, 1811
AfiI CCNNNNNNNGG 2 cut(s) 526, 615
AflII CTTAAG 1 cut(s) 74
AgsI TTSAA 3 cut(s) 382, 565, 1018
AhlI ACTAGT 1 cut(s) 602
AjnI CCWGG 4 cut(s) 157, 1002, 1187, 1470
AjuI GAANNNNNNNTTGG 2 cut(s) 891, 923
Alw21I GWGCWC 3 cut(s) 201, 704, 1780
Alw26I GTCTC 2 cut(s) 450, 801
AlwI GGATC 8 cut(s) 88, 94, 226, 293, 545, 782, 1405, 1436
AlwNI CAGNNNCTG 3 cut(s) 16, 686, 1373
Ama87I CYCGRG 2 cut(s) 764, 1687
AoxI GGCC 4 cut(s) 517, 1053, 1476, 1801
ApeKI GCWGC 9 cut(s) 164, 830, 1091, 1223, 1321, 1346, 1381, 1384, 1748
AseI ATTAAT 1 cut(s) 38
Asp700I GAANNNNTTC 2 cut(s) 336, 1135
AspLEI GCGC 2 cut(s) 1194, 1753
AspS9I GGNCC 2 cut(s) 517, 1666
AsuHPI GGTGA 4 cut(s) 283, 515, 809, 854
AsuII TTCGAA 1 cut(s) 1643
AvaI CYCGRG 2 cut(s) 764, 1687
AvaII GGWCC 1 cut(s) 1666
BaeGI GKGCMC 1 cut(s) 1555
BalI TGGCCA 1 cut(s) 1803
BanII GRGCYC 3 cut(s) 201, 704, 1780
BarI GAAGNNNNNNTAC 2 cut(s) 621, 653
BbrPI CACGTG 1 cut(s) 556
BbsI GAAGAC 2 cut(s) 674, 1856
Bbv12I GWGCWC 3 cut(s) 201, 704, 1780
BbvCI CCTCAGC 2 cut(s) 360, 372
BbvI GCAGC 9 cut(s) 151, 842, 1103, 1210, 1333, 1333, 1371, 1393, 1735
BccI CCATC 6 cut(s) 246, 262, 494, 847, 1336, 1694
BceAI ACGGC 3 cut(s) 504, 1228, 1530
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 4 cut(s) 159, 1004, 1189, 1472
BciVI GTATCC 1 cut(s) 1424
BclI TGATCA 1 cut(s) 1633
BcoDI GTCTC 2 cut(s) 450, 801
BcuI ACTAGT 1 cut(s) 602
BfaI CTAG 4 cut(s) 27, 366, 603, 1095
BfmI CTRYAG 1 cut(s) 792
BfoI RGCGCY 1 cut(s) 1195
BfrI CTTAAG 1 cut(s) 74
BfuAI ACCTGC 2 cut(s) 385, 421
BfuI GTATCC 1 cut(s) 1424
BglI GCCNNNNNGGC 1 cut(s) 161
BglII AGATCT 2 cut(s) 650, 1585
BisI GCNGC 9 cut(s) 165, 831, 1092, 1224, 1322, 1347, 1382, 1385, 1749
BlpI GCTNAGC 1 cut(s) 711
BlsI GCNGC 9 cut(s) 166, 832, 1093, 1225, 1323, 1348, 1383, 1386, 1750
Bme1390I CCNGG 4 cut(s) 159, 1004, 1189, 1472
Bme18I GGWCC 1 cut(s) 1666
BmeT110I CYCGRG 2 cut(s) 764, 1687
BmgT120I GGNCC 2 cut(s) 517, 1666
BmiI GGNNCC 1 cut(s) 1008
BmrFI CCNGG 4 cut(s) 159, 1004, 1189, 1472
BmsI GCATC 2 cut(s) 1153, 1615
BpiI GAAGAC 2 cut(s) 674, 1856
BpmI CTGGAG 2 cut(s) 1391, 1794
Bpu10I CCTNAGC 4 cut(s) 360, 372, 1299, 1458
Bpu1102I GCTNAGC 1 cut(s) 711
Bpu14I TTCGAA 1 cut(s) 1643
BpuEI CTTGAG 3 cut(s) 1010, 1121, 1805
Bsa29I ATCGAT 1 cut(s) 1841
BsaAI YACGTR 1 cut(s) 556
BsaJI CCNNGG 7 cut(s) 157, 158, 705, 1056, 1187, 1227, 1758
BsaWI WCCGGW 1 cut(s) 1780
BsaXI ACNNNNNCTCC 6 cut(s) 110, 140, 1389, 1419, 1767, 1797
Bsc4I CCNNNNNNNGG 2 cut(s) 526, 615
Bse1I ACTGG 3 cut(s) 780, 1617, 1804
Bse3DI GCAATG 1 cut(s) 1161
BseBI CCWGG 4 cut(s) 159, 1004, 1189, 1472
BseCI ATCGAT 1 cut(s) 1841
BseDI CCNNGG 7 cut(s) 157, 158, 705, 1056, 1187, 1227, 1758
BseGI GGATG 6 cut(s) 259, 505, 757, 910, 953, 1219
BseLI CCNNNNNNNGG 2 cut(s) 526, 615
BseMI GCAATG 1 cut(s) 1161
BseMII CTCAG 5 cut(s) 351, 363, 702, 1059, 1199
BseNI ACTGG 3 cut(s) 780, 1617, 1804
BseRI GAGGAG 4 cut(s) 45, 468, 902, 1073
BseSI GKGCMC 1 cut(s) 1555
BseXI GCAGC 9 cut(s) 151, 842, 1103, 1210, 1333, 1333, 1371, 1393, 1735
BseYI CCCAGC 1 cut(s) 659
BsgI GTGCAG 2 cut(s) 455, 1166
Bsh1285I CGRYCG 3 cut(s) 107, 1452, 1845
BshFI GGCC 4 cut(s) 519, 1055, 1478, 1803
BshVI ATCGAT 1 cut(s) 1841
BsiEI CGRYCG 3 cut(s) 107, 1452, 1845
BsiHKAI GWGCWC 3 cut(s) 201, 704, 1780
BsiHKCI CYCGRG 2 cut(s) 764, 1687
BsiSI CCGG 1 cut(s) 1781
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 2 cut(s) 526, 615
BsmAI GTCTC 2 cut(s) 450, 801
BsmFI GGGAC 1 cut(s) 459
BsnI GGCC 4 cut(s) 519, 1055, 1478, 1803
BsoBI CYCGRG 2 cut(s) 764, 1687
Bsp119I TTCGAA 1 cut(s) 1643
Bsp1286I GDGCHC 4 cut(s) 201, 704, 1555, 1780
Bsp1720I GCTNAGC 1 cut(s) 711
Bsp19I CCATGG 1 cut(s) 1758
BspACI CCGC 2 cut(s) 1448, 1664
BspANI GGCC 4 cut(s) 519, 1055, 1478, 1803
BspCNI CTCAG 5 cut(s) 352, 364, 703, 1060, 1200
BspDI ATCGAT 1 cut(s) 1841
BspHI TCATGA 2 cut(s) 1038, 1338
BspLI GGNNCC 1 cut(s) 1008
BspMI ACCTGC 2 cut(s) 385, 421
BspPI GGATC 8 cut(s) 88, 94, 226, 293, 545, 782, 1405, 1436
BspT104I TTCGAA 1 cut(s) 1643
BspTI CTTAAG 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 1161
BsrI ACTGG 3 cut(s) 780, 1617, 1804
BssECI CCNNGG 7 cut(s) 157, 158, 705, 1056, 1187, 1227, 1758
BssT1I CCWWGG 3 cut(s) 705, 1227, 1758
Bst2UI CCWGG 4 cut(s) 159, 1004, 1189, 1472
Bst6I CTCTTC 3 cut(s) 858, 888, 894
BstAFI CTTAAG 1 cut(s) 74
BstBAI YACGTR 1 cut(s) 556
BstBI TTCGAA 1 cut(s) 1643
BstC8I GCNNGC 5 cut(s) 976, 1116, 1221, 1351, 1399
BstDSI CCRYGG 1 cut(s) 1758
BstF5I GGATG 6 cut(s) 259, 505, 757, 910, 953, 1219
BstH2I RGCGCY 1 cut(s) 1195
BstHHI GCGC 2 cut(s) 1194, 1753
BstMAI GTCTC 2 cut(s) 450, 801
BstMCI CGRYCG 3 cut(s) 107, 1452, 1845
BstMWI GCNNNNNNNGC 7 cut(s) 161, 980, 1019, 1304, 1321, 1454, 1463
BstNI CCWGG 4 cut(s) 159, 1004, 1189, 1472
BstNSI RCATGY 2 cut(s) 54, 1223
BstSCI CCNGG 4 cut(s) 157, 1002, 1187, 1470
BstSFI CTRYAG 1 cut(s) 792
BstSLI GKGCMC 1 cut(s) 1555
BstV1I GCAGC 9 cut(s) 151, 842, 1103, 1210, 1333, 1333, 1371, 1393, 1735
BstV2I GAAGAC 2 cut(s) 674, 1856
BstX2I RGATCY 8 cut(s) 80, 231, 537, 650, 787, 1410, 1441, 1585
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 8 cut(s) 80, 231, 537, 650, 787, 1410, 1441, 1585
Bsu15I ATCGAT 1 cut(s) 1841
BsuI GTATCC 1 cut(s) 1424
BsuRI GGCC 4 cut(s) 519, 1055, 1478, 1803
BsuTUI ATCGAT 1 cut(s) 1841
BtgI CCRYGG 1 cut(s) 1758
BtsCI GGATG 6 cut(s) 259, 505, 757, 910, 953, 1219
BtsIMutI CAGTG 6 cut(s) 318, 1092, 1339, 1553, 1610, 1812
BveI ACCTGC 2 cut(s) 385, 421
Cac8I GCNNGC 5 cut(s) 976, 1116, 1221, 1351, 1399
CaiI CAGNNNCTG 3 cut(s) 16, 686, 1373
CciI TCATGA 2 cut(s) 1038, 1338
CfoI GCGC 2 cut(s) 1194, 1753
Cfr13I GGNCC 2 cut(s) 517, 1666
ClaI ATCGAT 1 cut(s) 1841
CseI GACGC 1 cut(s) 1861
Csp6I GTAC 3 cut(s) 579, 1389, 1810
CviAII CATG 9 cut(s) 51, 278, 958, 1039, 1220, 1339, 1394, 1759, 1786
CviQI GTAC 3 cut(s) 579, 1389, 1810
EaeI YGGCCR 2 cut(s) 1053, 1801
Eam1104I CTCTTC 3 cut(s) 858, 888, 894
EarI CTCTTC 3 cut(s) 858, 888, 894
Ecl136II GAGCTC 3 cut(s) 199, 702, 1778
Eco130I CCWWGG 3 cut(s) 705, 1227, 1758
Eco24I GRGCYC 3 cut(s) 201, 704, 1780
Eco47I GGWCC 1 cut(s) 1666
Eco53kI GAGCTC 3 cut(s) 199, 702, 1778
Eco57I CTGAAG 3 cut(s) 368, 623, 1301
Eco72I CACGTG 1 cut(s) 556
Eco88I CYCGRG 2 cut(s) 764, 1687
EcoICRI GAGCTC 3 cut(s) 199, 702, 1778
EcoRII CCWGG 4 cut(s) 157, 1002, 1187, 1470
EcoT14I CCWWGG 3 cut(s) 705, 1227, 1758
EcoT38I GRGCYC 3 cut(s) 201, 704, 1780
ErhI CCWWGG 3 cut(s) 705, 1227, 1758
FaeI CATG 9 cut(s) 54, 281, 961, 1042, 1223, 1342, 1397, 1762, 1789
FalI AAGNNNNNCTT 6 cut(s) 971, 1003, 1120, 1152, 1152, 1184
FaqI GGGAC 1 cut(s) 459
FatI CATG 9 cut(s) 50, 277, 957, 1038, 1219, 1338, 1393, 1758, 1785
FbaI TGATCA 1 cut(s) 1633
Fnu4HI GCNGC 9 cut(s) 165, 831, 1092, 1224, 1322, 1347, 1382, 1385, 1749
FokI GGATG 6 cut(s) 266, 512, 764, 917, 940, 1226
FriOI GRGCYC 3 cut(s) 201, 704, 1780
Fsp4HI GCNGC 9 cut(s) 165, 831, 1092, 1224, 1322, 1347, 1382, 1385, 1749
FspBI CTAG 4 cut(s) 27, 366, 603, 1095
GlaI GCGC 2 cut(s) 1193, 1752
GluI GCNGC 9 cut(s) 165, 831, 1092, 1224, 1322, 1347, 1382, 1385, 1749
GsaI CCCAGC 1 cut(s) 663
GsuI CTGGAG 2 cut(s) 1391, 1794
HaeII RGCGCY 1 cut(s) 1195
HaeIII GGCC 4 cut(s) 519, 1055, 1478, 1803
HapII CCGG 1 cut(s) 1781
HgaI GACGC 1 cut(s) 1861
HhaI GCGC 2 cut(s) 1194, 1753
Hin1II CATG 9 cut(s) 54, 281, 961, 1042, 1223, 1342, 1397, 1762, 1789
Hin6I GCGC 2 cut(s) 1192, 1751
HinP1I GCGC 2 cut(s) 1192, 1751
HindIII AAGCTT 3 cut(s) 635, 1073, 1562
HinfI GANTC 4 cut(s) 569, 767, 1082, 1404
HpaII CCGG 1 cut(s) 1781
HphI GGTGA 4 cut(s) 283, 515, 809, 854
Hpy166II GTNNAC 4 cut(s) 412, 1125, 1765, 1812
Hpy188I TCNGA 7 cut(s) 104, 228, 289, 642, 1000, 1495, 1656
Hpy8I GTNNAC 4 cut(s) 412, 1125, 1765, 1812
Hpy99I CGWCG 1 cut(s) 1256
HpyAV CCTTC 7 cut(s) 346, 433, 467, 508, 907, 934, 1420
HpyCH4IV ACGT 4 cut(s) 185, 555, 1050, 1329
HpyCH4V TGCA 7 cut(s) 54, 436, 1147, 1154, 1219, 1397, 1528
HpyF10VI GCNNNNNNNGC 7 cut(s) 161, 980, 1019, 1304, 1321, 1454, 1463
HpySE526I ACGT 4 cut(s) 185, 555, 1050, 1329
Hsp92II CATG 9 cut(s) 54, 281, 961, 1042, 1223, 1342, 1397, 1762, 1789
HspAI GCGC 2 cut(s) 1192, 1751
Ksp22I TGATCA 1 cut(s) 1633
LmnI GCTCC 5 cut(s) 707, 1370, 1775, 1783, 1860
Lsp1109I GCAGC 9 cut(s) 151, 842, 1103, 1210, 1333, 1333, 1371, 1393, 1735
LweI GCATC 2 cut(s) 1153, 1615
MaeI CTAG 4 cut(s) 27, 366, 603, 1095
MaeII ACGT 4 cut(s) 185, 555, 1050, 1329
MaeIII GTNAC 3 cut(s) 12, 488, 842
MfeI CAATTG 1 cut(s) 1197
MflI RGATCY 8 cut(s) 80, 231, 537, 650, 787, 1410, 1441, 1585
MhlI GDGCHC 4 cut(s) 201, 704, 1555, 1780
MlsI TGGCCA 1 cut(s) 1803
MluCI AATT 6 cut(s) 39, 377, 524, 1197, 1291, 1658
MluNI TGGCCA 1 cut(s) 1803
MlyI GAGTC 2 cut(s) 776, 1398
MmeI TCCRAC 2 cut(s) 567, 1582
Mox20I TGGCCA 1 cut(s) 1803
MroXI GAANNNNTTC 2 cut(s) 336, 1135
MscI TGGCCA 1 cut(s) 1803
MseI TTAA 5 cut(s) 38, 75, 1181, 1575, 1650
Msp20I TGGCCA 1 cut(s) 1803
MspA1I CMGCKG 1 cut(s) 1384
MspCI CTTAAG 1 cut(s) 74
MspI CCGG 1 cut(s) 1781
MspR9I CCNGG 4 cut(s) 159, 1004, 1189, 1472
MunI CAATTG 1 cut(s) 1197
MvaI CCWGG 4 cut(s) 159, 1004, 1189, 1472
MwoI GCNNNNNNNGC 7 cut(s) 161, 980, 1019, 1304, 1321, 1454, 1463
NcoI CCATGG 1 cut(s) 1758
NlaIII CATG 9 cut(s) 54, 281, 961, 1042, 1223, 1342, 1397, 1762, 1789
NlaIV GGNNCC 1 cut(s) 1008
NmeAIII GCCGAG 1 cut(s) 1081
NmuCI GTSAC 2 cut(s) 488, 842
NspI RCATGY 2 cut(s) 54, 1223
NspV TTCGAA 1 cut(s) 1643
PaeI GCATGC 1 cut(s) 1223
PagI TCATGA 2 cut(s) 1038, 1338
PaqCI CACCTGC 2 cut(s) 385, 421
PasI CCCWGGG 1 cut(s) 158
PcsI WCGNNNNNNNCGW 1 cut(s) 552
PdmI GAANNNNTTC 2 cut(s) 336, 1135
PfeI GAWTC 2 cut(s) 569, 1082
PfoI TCCNGGA 1 cut(s) 1470
PkrI GCNGC 9 cut(s) 166, 832, 1093, 1225, 1323, 1348, 1383, 1386, 1750
Ple19I CGATCG 2 cut(s) 107, 1845
PleI GAGTC 2 cut(s) 775, 1398
PmaCI CACGTG 1 cut(s) 556
PmlI CACGTG 1 cut(s) 556
PpsI GAGTC 2 cut(s) 775, 1398
Ppu21I YACGTR 1 cut(s) 556
PshBI ATTAAT 1 cut(s) 38
Psp124BI GAGCTC 3 cut(s) 201, 704, 1780
Psp6I CCWGG 4 cut(s) 157, 1002, 1187, 1470
PspCI CACGTG 1 cut(s) 556
PspFI CCCAGC 1 cut(s) 659
PspGI CCWGG 4 cut(s) 157, 1002, 1187, 1470
PspN4I GGNNCC 1 cut(s) 1008
PspPI GGNCC 2 cut(s) 517, 1666
PstNI CAGNNNCTG 3 cut(s) 16, 686, 1373
PsuI RGATCY 8 cut(s) 80, 231, 537, 650, 787, 1410, 1441, 1585
PvuI CGATCG 2 cut(s) 107, 1845
PvuII CAGCTG 1 cut(s) 1384
RsaI GTAC 3 cut(s) 580, 1390, 1811
RsaNI GTAC 3 cut(s) 579, 1389, 1810
SacI GAGCTC 3 cut(s) 201, 704, 1780
SaqAI TTAA 5 cut(s) 38, 75, 1181, 1575, 1650
SatI GCNGC 9 cut(s) 165, 831, 1092, 1224, 1322, 1347, 1382, 1385, 1749
Sau96I GGNCC 2 cut(s) 517, 1666
SchI GAGTC 2 cut(s) 776, 1398
ScrFI CCNGG 4 cut(s) 159, 1004, 1189, 1472
SduI GDGCHC 4 cut(s) 201, 704, 1555, 1780
SfaNI GCATC 2 cut(s) 1153, 1615
SfcI CTRYAG 1 cut(s) 792
SfuI TTCGAA 1 cut(s) 1643
SinI GGWCC 1 cut(s) 1666
SmlI CTYRAG 4 cut(s) 74, 1025, 1100, 1820
SmoI CTYRAG 4 cut(s) 74, 1025, 1100, 1820
SpeI ACTAGT 1 cut(s) 602
SphI GCATGC 1 cut(s) 1223
Sse9I AATT 6 cut(s) 39, 377, 524, 1197, 1291, 1658
SsiI CCGC 2 cut(s) 1448, 1664
SspI AATATT 1 cut(s) 817
SspMI CTAG 4 cut(s) 27, 366, 603, 1095
SstI GAGCTC 3 cut(s) 201, 704, 1780
StyD4I CCNGG 4 cut(s) 157, 1002, 1187, 1470
StyI CCWWGG 3 cut(s) 705, 1227, 1758
TaiI ACGT 4 cut(s) 188, 558, 1053, 1332
TaqII GACCGA 1 cut(s) 191
TasI AATT 6 cut(s) 39, 377, 524, 1197, 1291, 1658
TfiI GAWTC 2 cut(s) 569, 1082
Tru1I TTAA 5 cut(s) 38, 75, 1181, 1575, 1650
Tru9I TTAA 5 cut(s) 38, 75, 1181, 1575, 1650
TscAI CASTG 6 cut(s) 318, 1092, 1339, 1560, 1617, 1819
TseFI GTSAC 2 cut(s) 488, 842
TseI GCWGC 9 cut(s) 164, 830, 1091, 1223, 1321, 1346, 1381, 1384, 1748
Tsp45I GTSAC 2 cut(s) 488, 842
TspDTI ATGAA 5 cut(s) 270, 345, 486, 527, 942
TspGWI ACGGA 2 cut(s) 603, 819
TspRI CASTG 6 cut(s) 318, 1092, 1339, 1560, 1617, 1819
Vha464I CTTAAG 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 1666
VspI ATTAAT 1 cut(s) 38
XceI RCATGY 2 cut(s) 54, 1223
XcmI CCANNNNNNNNNTGG 1 cut(s) 971
XmnI GAANNNNTTC 2 cut(s) 336, 1135
XspI CTAG 4 cut(s) 27, 366, 603, 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.