AT5G03330

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001571
Physical Location & Seq
Forward (+)
806964 .. 810401
3438 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03330.3

Sequence Viewer

Length: 1071 bp
ATGGTGTCACATGAAGAGAACACAAGTATTGTGGAATGGTTTCTTGGTCCACATCCATACACGTATCCGCCTTATGGTATTGAGATGATTCATGAGGATGATGAAGTAGCAGTAGCTCATCATCATCATCATCACCAGAGCGGTGAGTATTACAGAGAGTATGAAGATCATCGTAGTAGCGATGTAGATAACGATGAGATCATTGCTAGAACTCTCCAAGACGATTTTTTGCAGCTTGAAATCGCAGAAAGTAACGATTATTCGCATCAGAATCAACAACAACAACATCAACAAGAAGGTTATACTAACAACTATAGTAATAACAACAATGGTTATGCTTGGAATGATCAGTCTCCTGCTGTGGATTATTCTTCAGAATGGATAGGAAATGATAATGATCAAGATGGGAGGAGCGATGATTCGGTTAATGTCTTTTCGTGTTCAAGCCCGAGTGACACGGATGAGTATGTATACTCGTGGGAATCGGATCAGTGTGATGCTGATGGTGAATTTGGAAGGAGATTGAATCAAATGGTTCCCATTCCGTATATACCGAAAATAAATGGAGAAATACCTCCGGAAGAAGAAGCAGTTTCAGATCATGAAAGACTTCGGAATAGGTTAGAGATGTTTGACTTCACTGAGGTTAAGGTTCCAGGAGATGGTAATTGCCAGTTCCGTGCTTTAGCTGATCAACTGTACAAAACCGCAGATCGCCATAAACATGTTCGACGACAAATAGTAAAACAGCTAAAATCTCGTCCAGATTCTTATCAAGGATACGTTCCTATGGACTTCTCTGATTATCTAAGAAAGATGTCTCGGAGCGGCGAATGGGGCGATCATGTGACATTACAGGCAGCTGCAGATGCGTATCGGGTGAAAATAGTAGTCCTAACATCGTTCAAAGATACATGTTACATTGAAATTCTTCCTACCTCCCAAGAATCCAAAGGAGTAATCTTCTTGAGCTTCTGGGCAGAGGTTCATTACAACGCCATCTACTTAAACAGAGATACATCTGAAACAGAGCTACAGAGGAAGAGAAAATGGTGGCGTTTCGGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

356

Amino Acids

41.69

Weight (kDa)

4.81

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 219 - 325 6.2e-14 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 662
AccBSI CCGCTC 2 cut(s) 141, 828
AccI GTMKAC 1 cut(s) 471
AccIII TCCGGA 1 cut(s) 577
AciI CCGC 4 cut(s) 68, 141, 708, 828
AclWI GGATC 1 cut(s) 495
AcsI RAATTY 2 cut(s) 509, 927
AcuI CTGAAG 1 cut(s) 357
AfaI GTAC 1 cut(s) 701
AfiI CCNNNNNNNGG 2 cut(s) 74, 662
AflIII ACRYGT 3 cut(s) 60, 724, 914
AgsI TTSAA 5 cut(s) 239, 444, 526, 907, 926
AjnI CCWGG 1 cut(s) 655
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 7 cut(s) 116, 235, 689, 751, 863, 972, 1033
AluI AGCT 7 cut(s) 116, 235, 689, 751, 863, 972, 1033
Alw26I GTCTC 2 cut(s) 357, 825
AlwI GGATC 1 cut(s) 495
Ama87I CYCGRG 1 cut(s) 448
Aor13HI TCCGGA 1 cut(s) 577
ApeKI GCWGC 3 cut(s) 232, 860, 863
ApoI RAATTY 2 cut(s) 509, 927
Asp700I GAANNNNTTC 3 cut(s) 39, 609, 930
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 4 cut(s) 125, 155, 518, 892
AvaI CYCGRG 1 cut(s) 448
AvaII GGWCC 1 cut(s) 47
BauI CACGAG 1 cut(s) 475
BbvI GCAGC 3 cut(s) 244, 850, 872
BccI CCATC 4 cut(s) 398, 497, 656, 1007
BciT130I CCWGG 1 cut(s) 657
BciVI GTATCC 2 cut(s) 75, 773
BclI TGATCA 3 cut(s) 346, 397, 691
BcoDI GTCTC 2 cut(s) 357, 825
BfaI CTAG 2 cut(s) 207, 1069
BfmI CTRYAG 3 cut(s) 313, 864, 1034
BfuI GTATCC 2 cut(s) 75, 773
BisI GCNGC 4 cut(s) 233, 829, 861, 864
BlsI GCNGC 4 cut(s) 234, 830, 862, 865
Bme1390I CCNGG 1 cut(s) 657
Bme18I GGWCC 1 cut(s) 47
BmeT110I CYCGRG 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 2 cut(s) 537, 654
BmrFI CCNGG 1 cut(s) 657
BmsI GCATC 3 cut(s) 274, 487, 859
BpuEI CTTGAG 1 cut(s) 988
BsaAI YACGTR 1 cut(s) 63
BsaBI GATNNNNATC 3 cut(s) 396, 771, 873
BsaWI WCCGGW 1 cut(s) 577
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 662
Bse1I ACTGG 1 cut(s) 673
Bse3DI GCAATG 1 cut(s) 201
Bse8I GATNNNNATC 3 cut(s) 396, 771, 873
BseAI TCCGGA 1 cut(s) 577
BseBI CCWGG 1 cut(s) 657
BseGI GGATG 3 cut(s) 52, 103, 466
BseJI GATNNNNATC 3 cut(s) 396, 771, 873
BseLI CCNNNNNNNGG 2 cut(s) 74, 662
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 1 cut(s) 673
BseRI GAGGAG 1 cut(s) 424
BseXI GCAGC 3 cut(s) 244, 850, 872
BsiHKCI CYCGRG 1 cut(s) 448
BsiSI CCGG 1 cut(s) 578
BslI CCNNNNNNNGG 2 cut(s) 74, 662
BsmAI GTCTC 2 cut(s) 357, 825
BsoBI CYCGRG 1 cut(s) 448
Bsp13I TCCGGA 1 cut(s) 577
Bsp1407I TGTACA 1 cut(s) 699
Bsp143I GATC 9 cut(s) 166, 198, 346, 397, 487, 598, 691, 712, 841
BspACI CCGC 4 cut(s) 68, 141, 708, 828
BspCNI CTCAG 1 cut(s) 634
BspEI TCCGGA 1 cut(s) 577
BspHI TCATGA 2 cut(s) 91, 601
BspLI GGNNCC 2 cut(s) 537, 654
BspMAI CTGCAG 1 cut(s) 868
BspPI GGATC 1 cut(s) 495
BsrBI CCGCTC 2 cut(s) 141, 828
BsrDI GCAATG 1 cut(s) 201
BsrGI TGTACA 1 cut(s) 699
BsrI ACTGG 1 cut(s) 673
BssMI GATC 9 cut(s) 166, 198, 346, 397, 487, 598, 691, 712, 841
BssNAI GTATAC 1 cut(s) 472
BssSI CACGAG 1 cut(s) 475
Bst1107I GTATAC 1 cut(s) 472
Bst2BI CACGAG 1 cut(s) 475
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 1 cut(s) 699
Bst6I CTCTTC 2 cut(s) 9, 1037
BstAUI TGTACA 1 cut(s) 699
BstBAI YACGTR 1 cut(s) 63
BstDEI CTNAG 2 cut(s) 642, 809
BstF5I GGATG 3 cut(s) 52, 103, 466
BstKTI GATC 9 cut(s) 169, 201, 349, 400, 490, 601, 694, 715, 844
BstMAI GTCTC 2 cut(s) 357, 825
BstMBI GATC 9 cut(s) 166, 198, 346, 397, 487, 598, 691, 712, 841
BstMWI GCNNNNNNNGC 2 cut(s) 837, 869
BstNI CCWGG 1 cut(s) 657
BstNSI RCATGY 2 cut(s) 728, 918
BstSCI CCNGG 1 cut(s) 655
BstSFI CTRYAG 3 cut(s) 313, 864, 1034
BstV1I GCAGC 3 cut(s) 244, 850, 872
BstZ17I GTATAC 1 cut(s) 472
BsuI GTATCC 2 cut(s) 75, 773
BtgZI GCGATG 2 cut(s) 195, 429
BtsCI GGATG 3 cut(s) 52, 103, 466
BtsIMutI CAGTG 2 cut(s) 497, 639
CciI TCATGA 2 cut(s) 91, 601
Cfr13I GGNCC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 700
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 6 cut(s) 11, 92, 602, 725, 845, 915
CviJI RGCY 8 cut(s) 116, 235, 447, 689, 751, 863, 972, 1033
CviKI_1 RGCY 8 cut(s) 116, 235, 447, 689, 751, 863, 972, 1033
CviQI GTAC 1 cut(s) 700
DdeI CTNAG 2 cut(s) 642, 809
DpnI GATC 9 cut(s) 168, 200, 348, 399, 489, 600, 693, 714, 843
DpnII GATC 9 cut(s) 166, 198, 346, 397, 487, 598, 691, 712, 841
Eam1104I CTCTTC 2 cut(s) 9, 1037
EarI CTCTTC 2 cut(s) 9, 1037
EciI GGCGGA 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 448
EcoRII CCWGG 1 cut(s) 655
FaeI CATG 6 cut(s) 14, 95, 605, 728, 848, 918
FatI CATG 6 cut(s) 10, 91, 601, 724, 844, 914
FbaI TGATCA 3 cut(s) 346, 397, 691
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 4 cut(s) 233, 829, 861, 864
FokI GGATG 3 cut(s) 39, 110, 473
Fsp4HI GCNGC 4 cut(s) 233, 829, 861, 864
FspBI CTAG 2 cut(s) 207, 1069
GluI GCNGC 4 cut(s) 233, 829, 861, 864
HapII CCGG 1 cut(s) 578
Hin1II CATG 6 cut(s) 14, 95, 605, 728, 848, 918
HinfI GANTC 7 cut(s) 88, 271, 419, 482, 526, 767, 947
HpaII CCGG 1 cut(s) 578
HphI GGTGA 4 cut(s) 125, 155, 518, 892
Hpy166II GTNNAC 2 cut(s) 50, 472
Hpy188I TCNGA 9 cut(s) 270, 376, 487, 598, 615, 802, 825, 1024, 1064
Hpy188III TCNNGA 6 cut(s) 92, 401, 578, 602, 764, 967
Hpy8I GTNNAC 2 cut(s) 50, 472
Hpy99I CGWCG 1 cut(s) 735
HpyAV CCTTC 2 cut(s) 290, 510
HpyCH4III ACNGT 1 cut(s) 699
HpyCH4IV ACGT 2 cut(s) 62, 783
HpyCH4V TGCA 2 cut(s) 232, 866
HpyF10VI GCNNNNNNNGC 2 cut(s) 837, 869
HpyF3I CTNAG 2 cut(s) 642, 809
HpySE526I ACGT 2 cut(s) 62, 783
Hsp92II CATG 6 cut(s) 14, 95, 605, 728, 848, 918
Kpn2I TCCGGA 1 cut(s) 577
Ksp22I TGATCA 3 cut(s) 346, 397, 691
Kzo9I GATC 9 cut(s) 166, 198, 346, 397, 487, 598, 691, 712, 841
LmnI GCTCC 2 cut(s) 411, 825
LpnPI CCDG 9 cut(s) 149, 369, 591, 642, 669, 686, 777, 842, 961
Lsp1109I GCAGC 3 cut(s) 244, 850, 872
LweI GCATC 3 cut(s) 274, 487, 859
MaeI CTAG 2 cut(s) 207, 1069
MaeII ACGT 2 cut(s) 62, 783
MaeIII GTNAC 5 cut(s) 6, 251, 452, 847, 917
MalI GATC 9 cut(s) 168, 200, 348, 399, 489, 600, 693, 714, 843
MbiI CCGCTC 2 cut(s) 141, 828
MboI GATC 9 cut(s) 166, 198, 346, 397, 487, 598, 691, 712, 841
MboII GAAGA 8 cut(s) 26, 176, 363, 593, 596, 923, 955, 1054
MluCI AATT 3 cut(s) 509, 667, 927
MnlI CCTC 7 cut(s) 88, 402, 585, 637, 949, 976, 1032
MroI TCCGGA 1 cut(s) 577
MroXI GAANNNNTTC 3 cut(s) 39, 609, 930
MseI TTAA 3 cut(s) 426, 648, 1007
MslI CAYNNNNRTG 2 cut(s) 96, 723
MspA1I CMGCKG 1 cut(s) 863
MspI CCGG 1 cut(s) 578
MspR9I CCNGG 1 cut(s) 657
MvaI CCWGG 1 cut(s) 657
MwoI GCNNNNNNNGC 2 cut(s) 837, 869
NdeII GATC 9 cut(s) 166, 198, 346, 397, 487, 598, 691, 712, 841
NlaIII CATG 6 cut(s) 14, 95, 605, 728, 848, 918
NlaIV GGNNCC 2 cut(s) 537, 654
NmuCI GTSAC 3 cut(s) 6, 452, 847
NspI RCATGY 2 cut(s) 728, 918
PagI TCATGA 2 cut(s) 91, 601
PciI ACATGT 2 cut(s) 724, 914
PdmI GAANNNNTTC 3 cut(s) 39, 609, 930
PfeI GAWTC 7 cut(s) 88, 271, 419, 482, 526, 767, 947
PflMI CCANNNNNTGG 1 cut(s) 662
PfoI TCCNGGA 1 cut(s) 655
PkrI GCNGC 4 cut(s) 234, 830, 862, 865
Ppu21I YACGTR 1 cut(s) 63
PscI ACATGT 2 cut(s) 724, 914
Psp6I CCWGG 1 cut(s) 655
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 2 cut(s) 537, 654
PspPI GGNCC 1 cut(s) 47
PstI CTGCAG 1 cut(s) 868
PvuII CAGCTG 1 cut(s) 863
RsaI GTAC 1 cut(s) 701
RsaNI GTAC 1 cut(s) 700
RseI CAYNNNNRTG 2 cut(s) 96, 723
SaqAI TTAA 3 cut(s) 426, 648, 1007
SatI GCNGC 4 cut(s) 233, 829, 861, 864
Sau3AI GATC 9 cut(s) 166, 198, 346, 397, 487, 598, 691, 712, 841
Sau96I GGNCC 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 657
SfaNI GCATC 3 cut(s) 274, 487, 859
SfcI CTRYAG 3 cut(s) 313, 864, 1034
SinI GGWCC 1 cut(s) 47
SmiMI CAYNNNNRTG 2 cut(s) 96, 723
SmlI CTYRAG 1 cut(s) 967
SmoI CTYRAG 1 cut(s) 967
Sse9I AATT 3 cut(s) 509, 667, 927
SsiI CCGC 4 cut(s) 68, 141, 708, 828
SspMI CTAG 2 cut(s) 207, 1069
StyD4I CCNGG 1 cut(s) 655
TaaI ACNGT 1 cut(s) 699
TaiI ACGT 2 cut(s) 65, 786
TaqI TCGA 1 cut(s) 730
TasI AATT 3 cut(s) 509, 667, 927
TatI WGTACW 1 cut(s) 699
TauI GCSGC 1 cut(s) 831
TfiI GAWTC 7 cut(s) 88, 271, 419, 482, 526, 767, 947
Tru1I TTAA 3 cut(s) 426, 648, 1007
Tru9I TTAA 3 cut(s) 426, 648, 1007
TscAI CASTG 2 cut(s) 497, 646
TseFI GTSAC 3 cut(s) 6, 452, 847
TseI GCWGC 3 cut(s) 232, 860, 863
Tsp45I GTSAC 3 cut(s) 6, 452, 847
TspDTI ATGAA 6 cut(s) 27, 80, 117, 177, 618, 977
TspGWI ACGGA 3 cut(s) 473, 534, 668
TspRI CASTG 2 cut(s) 497, 646
Van91I CCANNNNNTGG 1 cut(s) 662
VpaK11BI GGWCC 1 cut(s) 47
XapI RAATTY 2 cut(s) 509, 927
XceI RCATGY 2 cut(s) 728, 918
XmiI GTMKAC 1 cut(s) 471
XmnI GAANNNNTTC 3 cut(s) 39, 609, 930
XspI CTAG 2 cut(s) 207, 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.