FvH4_3g31490

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001571
Physical Location & Seq
Reverse (-)
25733207 .. 25741524
8318 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g31490.t4

Sequence Viewer

Length: 1047 bp
ATGCTTACATACGAGCAAGACCCGGATGTTGTTCGCTGGGGTCTCCAACTCTTTGAAGGTGATCCGTATTCGAATTGTGGGTATATTGACTCAGTTAATCAAGATGGCACGAATTATTATCCGGGGCGGTATTTTAAGGAAGATGTGTATGATACGGATTGTACAAATGTGGAATATGATGAACTCATTGCTCATGCTCTTCAAGAGGAGCTATCATTACTTGATATAGAGGAAGAGCCTGGATCTTCACCTGAACAAGTTGAGCATTTGCAAGCAACTGTCTTCCCACAAGATTGGTCGCAGCATTCTATTGGAAATTATGGCTCTGGACAGGAGATTGTTCAGGCAGAGGCAGATGAAGTTGGGCCTTCTAGTTCATGTTCTAGCCCAGTTGAGAAGTCATACTATGGAGATGATTGGTCATATTCCCTAGAGCTGGCAAATGAATATACTTTTGATGGAGAGAGGTTGAACCAGATGGTTCCTGTTCCTCATGTTCCTAAAATCAATGGTGATATACCCTCGCTTGATCAAGCAACTTTAGATCACCAAAGGCTTCTGGACAGGTTGCAGTTATATGGGTTGGTTGAAAATAAAGTCCAAGGTGATGGAAATTGTCAGTTCCGCGCTCTAGCTGACCAATTTTATCAATCTCCTGAACACCACGAATTTGTGAGACAACAAATTGTAAATCAGCTTAAGTCATACCCAGAGATATATGAAGGATATGTTCCCATGGCTTATGATGAATATTTGGAGAAGATGTCGAGGAGTGGTGAGTGGGGCGATCATGTTACTTTACAGGCTGCTGCAGATTTGTATGGAGTTAAAATTTTTGTCATAACTTCTTTCAAGGACACCTGCTACATTGAGATACTTCCTAGTGCTGAAAGGTCAACACGAGTAATTTGTCTGAGCTTTTGGGCAGAGGTGCACTACAACTCAATTTATTCTGAAGGAGATGTGCCTACATTTGAGACGAAGAAGAAGAAGAAAAGGGGGACGTCACGTAATAAGCACTTGGAGGTCTCAAATATATACCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

349

Amino Acids

39.89

Weight (kDa)

4.5

Isoelectric Point (pI)

52.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 201 - 287 1.3e-10 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 869
AatII GACGTC 1 cut(s) 1007
Acc36I ACCTGC 1 cut(s) 869
AccII CGCG 1 cut(s) 627
AciI CCGC 2 cut(s) 127, 625
AclWI GGATC 2 cut(s) 56, 250
AcsI RAATTY 2 cut(s) 668, 831
AcuI CTGAAG 1 cut(s) 975
AcyI GRCGYC 1 cut(s) 1004
AfaI GTAC 1 cut(s) 163
AfiI CCNNNNNNNGG 1 cut(s) 436
AflII CTTAAG 1 cut(s) 698
AgsI TTSAA 5 cut(s) 56, 203, 472, 590, 853
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 5 cut(s) 211, 436, 635, 697, 918
AluI AGCT 5 cut(s) 211, 436, 635, 697, 918
Alw21I GWGCWC 1 cut(s) 936
Alw26I GTCTC 4 cut(s) 47, 670, 971, 1033
Alw44I GTGCAC 1 cut(s) 932
AlwI GGATC 2 cut(s) 56, 250
AoxI GGCC 1 cut(s) 365
ApaLI GTGCAC 1 cut(s) 932
ApeKI GCWGC 3 cut(s) 301, 806, 809
ApoI RAATTY 2 cut(s) 668, 831
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 1 cut(s) 365
AsuC2I CCSGG 2 cut(s) 23, 123
AsuHPI GGTGA 6 cut(s) 71, 240, 524, 539, 617, 788
AsuII TTCGAA 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 936
BauI CACGAG 1 cut(s) 900
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 936
BbvI GCAGC 3 cut(s) 313, 793, 796
BccI CCATC 4 cut(s) 98, 452, 472, 602
BciT130I CCWGG 1 cut(s) 240
BclI TGATCA 1 cut(s) 529
BcnI CCSGG 2 cut(s) 23, 123
BcoDI GTCTC 4 cut(s) 47, 670, 971, 1033
BfaI CTAG 5 cut(s) 372, 384, 431, 632, 882
BfmI CTRYAG 1 cut(s) 810
BfrI CTTAAG 1 cut(s) 698
BfuAI ACCTGC 1 cut(s) 869
BisI GCNGC 3 cut(s) 302, 807, 810
BlsI GCNGC 3 cut(s) 303, 808, 811
Bme1390I CCNGG 3 cut(s) 23, 123, 240
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 1 cut(s) 483
BmrFI CCNGG 3 cut(s) 23, 123, 240
BmrI ACTGGG 1 cut(s) 383
BmuI ACTGGG 1 cut(s) 383
BpiI GAAGAC 1 cut(s) 274
Bpu14I TTCGAA 1 cut(s) 71
BpuMI CCSGG 2 cut(s) 23, 123
BsaAI YACGTR 1 cut(s) 1010
BsaHI GRCGYC 1 cut(s) 1004
BsaI GGTCTC 2 cut(s) 47, 1033
BsaJI CCNNGG 3 cut(s) 122, 601, 735
BsaXI ACNNNNNCTCC 2 cut(s) 749, 779
Bsc4I CCNNNNNNNGG 1 cut(s) 436
Bse1I ACTGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 186
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 3 cut(s) 122, 601, 735
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 1 cut(s) 436
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 105, 905
BseNI ACTGG 1 cut(s) 389
BseRI GAGGAG 2 cut(s) 221, 784
BseSI GKGCMC 1 cut(s) 936
BseXI GCAGC 3 cut(s) 313, 793, 796
BseYI CCCAGC 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 627
BshFI GGCC 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 936
BsiSI CCGG 2 cut(s) 23, 122
BslFI GGGAC 1 cut(s) 1015
BslI CCNNNNNNNGG 1 cut(s) 436
BsmAI GTCTC 4 cut(s) 47, 670, 971, 1033
BsmBI CGTCTC 1 cut(s) 971
BsmFI GGGAC 1 cut(s) 1015
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 1 cut(s) 367
Bso31I GGTCTC 2 cut(s) 47, 1033
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 936
Bsp1407I TGTACA 1 cut(s) 161
Bsp143I GATC 5 cut(s) 61, 242, 529, 544, 787
Bsp19I CCATGG 1 cut(s) 735
BspACI CCGC 2 cut(s) 127, 625
BspANI GGCC 1 cut(s) 367
BspCNI CTCAG 2 cut(s) 104, 906
BspFNI CGCG 1 cut(s) 627
BspLI GGNNCC 1 cut(s) 483
BspMAI CTGCAG 1 cut(s) 814
BspMI ACCTGC 1 cut(s) 869
BspPI GGATC 2 cut(s) 56, 250
BspQI GCTCTTC 2 cut(s) 204, 228
BspT104I TTCGAA 1 cut(s) 71
BspTI CTTAAG 1 cut(s) 698
BspTNI GGTCTC 2 cut(s) 47, 1033
BsrDI GCAATG 1 cut(s) 186
BsrGI TGTACA 1 cut(s) 161
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 3 cut(s) 122, 601, 735
BssMI GATC 5 cut(s) 61, 242, 529, 544, 787
BssNI GRCGYC 1 cut(s) 1004
BssSI CACGAG 1 cut(s) 900
BssT1I CCWWGG 2 cut(s) 601, 735
Bst2BI CACGAG 1 cut(s) 900
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 2 cut(s) 204, 228
BstACI GRCGYC 1 cut(s) 1004
BstAFI CTTAAG 1 cut(s) 698
BstAUI TGTACA 1 cut(s) 161
BstBAI YACGTR 1 cut(s) 1010
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 273, 438
BstDEI CTNAG 2 cut(s) 91, 914
BstDSI CCRYGG 1 cut(s) 735
BstF5I GGATG 1 cut(s) 31
BstFNI CGCG 1 cut(s) 627
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 5 cut(s) 64, 245, 532, 547, 790
BstMAI GTCTC 4 cut(s) 47, 670, 971, 1033
BstMBI GATC 5 cut(s) 61, 242, 529, 544, 787
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 3 cut(s) 21, 121, 238
BstSFI CTRYAG 1 cut(s) 810
BstSLI GKGCMC 1 cut(s) 936
BstUI CGCG 1 cut(s) 627
BstV1I GCAGC 3 cut(s) 313, 793, 796
BstV2I GAAGAC 1 cut(s) 274
BstX2I RGATCY 1 cut(s) 242
BstXI CCANNNNNNTGG 2 cut(s) 294, 608
BstYI RGATCY 1 cut(s) 242
BsuRI GGCC 1 cut(s) 367
BtgI CCRYGG 1 cut(s) 735
BtsCI GGATG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 1042
BveI ACCTGC 1 cut(s) 869
Cac8I GCNNGC 2 cut(s) 273, 438
CfoI GCGC 1 cut(s) 629
Cfr13I GGNCC 1 cut(s) 365
Csp6I GTAC 1 cut(s) 162
CviAII CATG 5 cut(s) 194, 378, 494, 736, 791
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 2 cut(s) 91, 914
DpnI GATC 5 cut(s) 63, 244, 531, 546, 789
DpnII GATC 5 cut(s) 61, 242, 529, 544, 787
Eam1104I CTCTTC 2 cut(s) 204, 228
EarI CTCTTC 2 cut(s) 204, 228
Eco130I CCWWGG 2 cut(s) 601, 735
Eco31I GGTCTC 2 cut(s) 47, 1033
Eco57I CTGAAG 1 cut(s) 975
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 2 cut(s) 601, 735
ErhI CCWWGG 2 cut(s) 601, 735
Esp3I CGTCTC 1 cut(s) 971
FaeI CATG 5 cut(s) 197, 381, 497, 739, 794
FaqI GGGAC 1 cut(s) 1015
FatI CATG 5 cut(s) 193, 377, 493, 735, 790
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 3 cut(s) 302, 807, 810
FokI GGATG 1 cut(s) 38
Fsp4HI GCNGC 3 cut(s) 302, 807, 810
FspBI CTAG 5 cut(s) 372, 384, 431, 632, 882
GlaI GCGC 1 cut(s) 628
GluI GCNGC 3 cut(s) 302, 807, 810
GsaI CCCAGC 1 cut(s) 40
HaeIII GGCC 1 cut(s) 367
HapII CCGG 2 cut(s) 23, 122
HhaI GCGC 1 cut(s) 629
Hin1I GRCGYC 1 cut(s) 1004
Hin1II CATG 5 cut(s) 197, 381, 497, 739, 794
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HincII GTYRAC 1 cut(s) 897
HindII GTYRAC 1 cut(s) 897
HinfI GANTC 1 cut(s) 89
HpaII CCGG 2 cut(s) 23, 122
HphI GGTGA 6 cut(s) 71, 240, 524, 539, 617, 788
Hpy166II GTNNAC 2 cut(s) 897, 934
Hpy188I TCNGA 2 cut(s) 915, 955
Hpy188III TCNNGA 5 cut(s) 101, 203, 327, 560, 656
Hpy8I GTNNAC 2 cut(s) 897, 934
HpyAV CCTTC 4 cut(s) 50, 378, 716, 950
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 2 cut(s) 1004, 1009
HpyCH4V TGCA 4 cut(s) 271, 571, 812, 934
HpyF3I CTNAG 2 cut(s) 91, 914
HpySE526I ACGT 2 cut(s) 1004, 1009
Hsp92I GRCGYC 1 cut(s) 1004
Hsp92II CATG 5 cut(s) 197, 381, 497, 739, 794
HspAI GCGC 1 cut(s) 627
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 5 cut(s) 61, 242, 529, 544, 787
LguI GCTCTTC 2 cut(s) 204, 228
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 3 cut(s) 313, 793, 796
MaeI CTAG 5 cut(s) 372, 384, 431, 632, 882
MaeII ACGT 2 cut(s) 1004, 1009
MaeIII GTNAC 2 cut(s) 793, 1005
MalI GATC 5 cut(s) 63, 244, 531, 546, 789
MboI GATC 5 cut(s) 61, 242, 529, 544, 787
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 936
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 9 cut(s) 199, 223, 343, 459, 501, 532, 762, 922, 1018
MseI TTAA 4 cut(s) 96, 135, 699, 828
MspCI CTTAAG 1 cut(s) 698
MspI CCGG 2 cut(s) 23, 122
MspR9I CCNGG 3 cut(s) 23, 123, 240
Mva1269I GAATGC 1 cut(s) 304
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 1 cut(s) 627
NciI CCSGG 2 cut(s) 23, 123
NcoI CCATGG 1 cut(s) 735
NdeII GATC 5 cut(s) 61, 242, 529, 544, 787
NlaIII CATG 5 cut(s) 197, 381, 497, 739, 794
NlaIV GGNNCC 1 cut(s) 483
NmuCI GTSAC 1 cut(s) 1005
NspV TTCGAA 1 cut(s) 71
PaqCI CACCTGC 1 cut(s) 869
PciSI GCTCTTC 2 cut(s) 204, 228
PctI GAATGC 1 cut(s) 304
PkrI GCNGC 3 cut(s) 303, 808, 811
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
Ppu21I YACGTR 1 cut(s) 1010
Psp6I CCWGG 1 cut(s) 238
PspFI CCCAGC 1 cut(s) 36
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 1 cut(s) 365
PstI CTGCAG 1 cut(s) 814
PsuI RGATCY 1 cut(s) 242
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
SapI GCTCTTC 2 cut(s) 204, 228
SaqAI TTAA 4 cut(s) 96, 135, 699, 828
SatI GCNGC 3 cut(s) 302, 807, 810
Sau3AI GATC 5 cut(s) 61, 242, 529, 544, 787
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 3 cut(s) 23, 123, 240
SduI GDGCHC 1 cut(s) 936
SfcI CTRYAG 1 cut(s) 810
SfuI TTCGAA 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 698
SmoI CTYRAG 1 cut(s) 698
SsiI CCGC 2 cut(s) 127, 625
SspI AATATT 1 cut(s) 752
SspMI CTAG 5 cut(s) 372, 384, 431, 632, 882
StyD4I CCNGG 3 cut(s) 21, 121, 238
StyI CCWWGG 2 cut(s) 601, 735
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 2 cut(s) 1007, 1012
TaqI TCGA 2 cut(s) 71, 767
TatI WGTACW 1 cut(s) 161
Tru1I TTAA 4 cut(s) 96, 135, 699, 828
Tru9I TTAA 4 cut(s) 96, 135, 699, 828
TseFI GTSAC 1 cut(s) 1005
TseI GCWGC 3 cut(s) 301, 806, 809
Tsp45I GTSAC 1 cut(s) 1005
TspDTI ATGAA 6 cut(s) 195, 366, 372, 459, 735, 762
TspGWI ACGGA 2 cut(s) 54, 170
Vha464I CTTAAG 1 cut(s) 698
VneI GTGCAC 1 cut(s) 932
XapI RAATTY 2 cut(s) 668, 831
XspI CTAG 5 cut(s) 372, 384, 431, 632, 882
ZraI GACGTC 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.