MD12G1115200.v1.1

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001571
Physical Location & Seq
Reverse (-)
18366372 .. 18371153
4782 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1115200.v1.1.491

Sequence Viewer

Length: 996 bp
ATGACTTCCCATGAGTACGATTCAGATATTGTTCAGTGGGGTATTCGTCTTTTTGGTAGGGATCCAGTTTGTAATTCTTCATACTACTATGGTGACATGATACAACACGATTCCAATGATATCGACCATGAAGAGTGTATCAGAAGTCATTATGATACAGAATCAAACAATGTAGAAAATGATGAGATCATTGCACATACTCTTCAAGAAGAATTTTCGCGACTATCTGTAACAGAGGCCTCTGAATACTCCCATGCAGGAGAAGCGCAATCACAAGCTTCATTTCTTGCAGATGAGTGGCATGGTCAATCTACAAGAGATTATTACTCAGACCATGGTTATGGCCAGGAAGAAGCTGATACTGTGGTGCCTTCTAGCTTATGTTCTAGACCTGATGATCATTTGGAGCTAACTGATGGATGTGCACTTGATGGTGAAGTAGGCTGGGGCTGGAATCCTGTTCCTCACATTCCTAGAATCAATGGAGAAATTCCTTCAATTGATGAAGCGACTTCAGATCATCAAAGGCTAGTTGACAGGCTTCAATTATATGGCTTCGTCGAACACAAGGTGGCAGGAGATGGCAACTGCCAGTTCCATGCTTTATCAGACCAGTTGTATCAATCACCTGACAACCACGAGTATGTGAGAAAGCAAGTTGTAAATCAGCTTAAGTCTCATCCAGAGATATACGAGGGATATGTTCCTATGGCATATGATGACTATTTGGAGAAGATGTCCAAGGATGGTGAGTGGGGTGACCATGTCTCATTACAGGCGGCTGCTGATTTGTACGAAGTGAAAATATTTGTCTTGACGTCGTTTAAGGATAACTGTTGCATAGAGATTCTTCCTAATATTCAAACTTCAAAACAAGTTATTTATTTGAGTTTCTGGGCGGAGGTGCATTACAACTCAATTTATCCTCAAGGAGGCACACCCTCATATGAGAACAGGAAAAAGAAAAAATGGCGGATATTTGGGAATAAGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

332

Amino Acids

38.02

Weight (kDa)

4.72

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 193 - 279 1.9e-14 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 821
AccB1I GGYRCC 1 cut(s) 367
AccII CGCG 1 cut(s) 220
AciI CCGC 3 cut(s) 779, 899, 973
AclWI GGATC 2 cut(s) 56, 69
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 2 cut(s) 212, 489
AcuI CTGAAG 1 cut(s) 498
AcyI GRCGYC 1 cut(s) 818
AdeI CACNNNGTG 1 cut(s) 571
AfaI GTAC 2 cut(s) 17, 794
AfiI CCNNNNNNNGG 1 cut(s) 932
AflII CTTAAG 1 cut(s) 671
AgsI TTSAA 5 cut(s) 206, 498, 545, 863, 870
AjnI CCWGG 1 cut(s) 345
AluBI AGCT 5 cut(s) 278, 356, 378, 409, 670
AluI AGCT 5 cut(s) 278, 356, 378, 409, 670
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 2 cut(s) 681, 772
Alw44I GTGCAC 1 cut(s) 423
AlwI GGATC 2 cut(s) 56, 69
AoxI GGCC 2 cut(s) 237, 343
ApaLI GTGCAC 1 cut(s) 423
ApeKI GCWGC 1 cut(s) 782
ApoI RAATTY 2 cut(s) 212, 489
AspLEI GCGC 1 cut(s) 268
AsuHPI GGTGA 5 cut(s) 104, 446, 618, 761, 770
BaeGI GKGCMC 1 cut(s) 427
BaeI ACNNNNGTAYC 2 cut(s) 351, 384
BalI TGGCCA 1 cut(s) 345
BamHI GGATCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 367
BauI CACGAG 1 cut(s) 638
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 1 cut(s) 769
BccI CCATC 4 cut(s) 410, 425, 575, 740
BciT130I CCWGG 1 cut(s) 347
BclI TGATCA 1 cut(s) 397
BcoDI GTCTC 2 cut(s) 681, 772
BfaI CTAG 4 cut(s) 375, 387, 474, 530
BfrI CTTAAG 1 cut(s) 671
BisI GCNGC 2 cut(s) 780, 783
BlsI GCNGC 2 cut(s) 781, 784
Bme1390I CCNGG 1 cut(s) 347
BmiI GGNNCC 2 cut(s) 63, 369
BmrFI CCNGG 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 912
BsaHI GRCGYC 1 cut(s) 818
BsaJI CCNNGG 2 cut(s) 334, 741
BsaXI ACNNNNNCTCC 2 cut(s) 722, 752
Bsc4I CCNNNNNNNGG 1 cut(s) 932
Bse1I ACTGG 3 cut(s) 65, 592, 613
Bse3DI GCAATG 1 cut(s) 189
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 2 cut(s) 334, 741
BseGI GGATG 3 cut(s) 425, 679, 751
BseLI CCNNNNNNNGG 1 cut(s) 932
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 3 cut(s) 65, 592, 613
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 1 cut(s) 769
BseYI CCCAGC 1 cut(s) 444
Bsh1236I CGCG 1 cut(s) 220
BshFI GGCC 2 cut(s) 239, 345
BshNI GGYRCC 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 427
BslI CCNNNNNNNGG 1 cut(s) 932
BsmAI GTCTC 2 cut(s) 681, 772
BsnI GGCC 2 cut(s) 239, 345
Bsp1286I GDGCHC 1 cut(s) 427
Bsp143I GATC 4 cut(s) 61, 186, 397, 517
Bsp19I CCATGG 1 cut(s) 334
Bsp68I TCGCGA 1 cut(s) 220
BspACI CCGC 3 cut(s) 779, 899, 973
BspANI GGCC 2 cut(s) 239, 345
BspCNI CTCAG 1 cut(s) 341
BspFNI CGCG 1 cut(s) 220
BspLI GGNNCC 2 cut(s) 63, 369
BspPI GGATC 2 cut(s) 56, 69
BspT107I GGYRCC 1 cut(s) 367
BspTI CTTAAG 1 cut(s) 671
BsrDI GCAATG 1 cut(s) 189
BsrI ACTGG 3 cut(s) 65, 592, 613
BssECI CCNNGG 2 cut(s) 334, 741
BssMI GATC 4 cut(s) 61, 186, 397, 517
BssNI GRCGYC 1 cut(s) 818
BssSI CACGAG 1 cut(s) 638
BssT1I CCWWGG 2 cut(s) 334, 741
Bst2BI CACGAG 1 cut(s) 638
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 2 cut(s) 364, 836
Bst6I CTCTTC 2 cut(s) 126, 207
BstACI GRCGYC 1 cut(s) 818
BstAFI CTTAAG 1 cut(s) 671
BstDEI CTNAG 1 cut(s) 328
BstDSI CCRYGG 1 cut(s) 334
BstEII GGTNACC 1 cut(s) 758
BstENI CCTNNNNNAGG 1 cut(s) 930
BstF5I GGATG 3 cut(s) 425, 679, 751
BstFNI CGCG 1 cut(s) 220
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 4 cut(s) 64, 189, 400, 520
BstMAI GTCTC 2 cut(s) 681, 772
BstMBI GATC 4 cut(s) 61, 186, 397, 517
BstMWI GCNNNNNNNGC 1 cut(s) 263
BstNI CCWGG 1 cut(s) 347
BstPI GGTNACC 1 cut(s) 758
BstSCI CCNGG 1 cut(s) 345
BstSLI GKGCMC 1 cut(s) 427
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 769
BstX2I RGATCY 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 341
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 2 cut(s) 239, 345
BtgI CCRYGG 1 cut(s) 334
BtsCI GGATG 3 cut(s) 425, 679, 751
BtsIMutI CAGTG 1 cut(s) 41
BtuMI TCGCGA 1 cut(s) 220
CfoI GCGC 1 cut(s) 268
Csp6I GTAC 2 cut(s) 16, 793
CviAII CATG 8 cut(s) 11, 97, 128, 254, 302, 335, 599, 764
CviQI GTAC 2 cut(s) 16, 793
DdeI CTNAG 1 cut(s) 328
DpnI GATC 4 cut(s) 63, 188, 399, 519
DpnII GATC 4 cut(s) 61, 186, 397, 517
DraIII CACNNNGTG 1 cut(s) 571
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 2 cut(s) 126, 207
EarI CTCTTC 2 cut(s) 126, 207
EciI GGCGGA 2 cut(s) 914, 988
Eco130I CCWWGG 2 cut(s) 334, 741
Eco147I AGGCCT 1 cut(s) 239
Eco32I GATATC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 498
Eco91I GGTNACC 1 cut(s) 758
EcoNI CCTNNNNNAGG 1 cut(s) 930
EcoO65I GGTNACC 1 cut(s) 758
EcoRII CCWGG 1 cut(s) 345
EcoRV GATATC 1 cut(s) 121
EcoT14I CCWWGG 2 cut(s) 334, 741
ErhI CCWWGG 2 cut(s) 334, 741
FaeI CATG 8 cut(s) 14, 100, 131, 257, 305, 338, 602, 767
FatI CATG 8 cut(s) 10, 96, 127, 253, 301, 334, 598, 763
FauNDI CATATG 2 cut(s) 715, 946
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 780, 783
FokI GGATG 3 cut(s) 432, 666, 758
Fsp4HI GCNGC 2 cut(s) 780, 783
FspBI CTAG 4 cut(s) 375, 387, 474, 530
GlaI GCGC 1 cut(s) 267
GluI GCNGC 2 cut(s) 780, 783
GsaI CCCAGC 1 cut(s) 448
HaeIII GGCC 2 cut(s) 239, 345
HhaI GCGC 1 cut(s) 268
Hin1I GRCGYC 1 cut(s) 818
Hin1II CATG 8 cut(s) 14, 100, 131, 257, 305, 338, 602, 767
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HindIII AAGCTT 1 cut(s) 276
HinfI GANTC 6 cut(s) 20, 110, 161, 454, 477, 847
HphI GGTGA 5 cut(s) 104, 446, 618, 761, 770
Hpy166II GTNNAC 2 cut(s) 425, 535
Hpy188I TCNGA 6 cut(s) 25, 143, 244, 331, 517, 610
Hpy188III TCNNGA 5 cut(s) 206, 219, 387, 683, 814
Hpy8I GTNNAC 2 cut(s) 425, 535
Hpy99I CGWCG 2 cut(s) 563, 823
HpyAV CCTTC 2 cut(s) 381, 504
HpyCH4III ACNGT 2 cut(s) 364, 836
HpyCH4IV ACGT 1 cut(s) 818
HpyCH4V TGCA 6 cut(s) 194, 257, 290, 425, 840, 907
HpyF10VI GCNNNNNNNGC 1 cut(s) 263
HpyF3I CTNAG 1 cut(s) 328
HpySE526I ACGT 1 cut(s) 818
Hsp92I GRCGYC 1 cut(s) 818
Hsp92II CATG 8 cut(s) 14, 100, 131, 257, 305, 338, 602, 767
HspAI GCGC 1 cut(s) 266
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 4 cut(s) 61, 186, 397, 517
LmnI GCTCC 1 cut(s) 406
Lsp1109I GCAGC 1 cut(s) 769
MaeI CTAG 4 cut(s) 375, 387, 474, 530
MaeII ACGT 1 cut(s) 818
MaeIII GTNAC 3 cut(s) 92, 229, 758
MalI GATC 4 cut(s) 63, 188, 399, 519
MboI GATC 4 cut(s) 61, 186, 397, 517
MboII GAAGA 7 cut(s) 69, 143, 194, 221, 362, 745, 842
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 1 cut(s) 427
MlsI TGGCCA 1 cut(s) 345
MluCI AATT 6 cut(s) 73, 212, 489, 498, 545, 918
MluNI TGGCCA 1 cut(s) 345
MnlI CCTC 8 cut(s) 229, 250, 474, 688, 895, 926, 936, 952
Mox20I TGGCCA 1 cut(s) 345
MscI TGGCCA 1 cut(s) 345
MseI TTAA 2 cut(s) 672, 825
MslI CAYNNNNRTG 2 cut(s) 339, 642
Msp20I TGGCCA 1 cut(s) 345
MspCI CTTAAG 1 cut(s) 671
MspR9I CCNGG 1 cut(s) 347
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 347
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 1 cut(s) 263
NcoI CCATGG 1 cut(s) 334
NdeI CATATG 2 cut(s) 715, 946
NdeII GATC 4 cut(s) 61, 186, 397, 517
NlaIII CATG 8 cut(s) 14, 100, 131, 257, 305, 338, 602, 767
NlaIV GGNNCC 2 cut(s) 63, 369
NmuCI GTSAC 2 cut(s) 92, 758
NruI TCGCGA 1 cut(s) 220
PceI AGGCCT 1 cut(s) 239
PfeI GAWTC 6 cut(s) 20, 110, 161, 454, 477, 847
PflFI GACNNNGTC 1 cut(s) 764
PkrI GCNGC 2 cut(s) 781, 784
Psp6I CCWGG 1 cut(s) 345
PspEI GGTNACC 1 cut(s) 758
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 63, 369
PsuI RGATCY 1 cut(s) 61
PsyI GACNNNGTC 1 cut(s) 764
RruI TCGCGA 1 cut(s) 220
RsaI GTAC 2 cut(s) 17, 794
RsaNI GTAC 2 cut(s) 16, 793
RseI CAYNNNNRTG 2 cut(s) 339, 642
SaqAI TTAA 2 cut(s) 672, 825
SatI GCNGC 2 cut(s) 780, 783
Sau3AI GATC 4 cut(s) 61, 186, 397, 517
ScrFI CCNGG 1 cut(s) 347
SduI GDGCHC 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 339, 642
SmlI CTYRAG 2 cut(s) 671, 927
SmoI CTYRAG 2 cut(s) 671, 927
Sse9I AATT 6 cut(s) 73, 212, 489, 498, 545, 918
SseBI AGGCCT 1 cut(s) 239
SsiI CCGC 3 cut(s) 779, 899, 973
SspI AATATT 2 cut(s) 807, 859
SspMI CTAG 4 cut(s) 375, 387, 474, 530
StuI AGGCCT 1 cut(s) 239
StyD4I CCNGG 1 cut(s) 345
StyI CCWWGG 2 cut(s) 334, 741
TaaI ACNGT 2 cut(s) 364, 836
TaiI ACGT 1 cut(s) 821
TaqI TCGA 2 cut(s) 123, 561
TasI AATT 6 cut(s) 73, 212, 489, 498, 545, 918
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 6 cut(s) 20, 110, 161, 454, 477, 847
Tru1I TTAA 2 cut(s) 672, 825
Tru9I TTAA 2 cut(s) 672, 825
TscAI CASTG 1 cut(s) 41
TseFI GTSAC 2 cut(s) 92, 758
TseI GCWGC 1 cut(s) 782
Tsp45I GTSAC 2 cut(s) 92, 758
TspDTI ATGAA 4 cut(s) 69, 144, 270, 519
TspRI CASTG 1 cut(s) 41
Tth111I GACNNNGTC 1 cut(s) 764
Vha464I CTTAAG 1 cut(s) 671
VneI GTGCAC 1 cut(s) 423
XagI CCTNNNNNAGG 1 cut(s) 930
XapI RAATTY 2 cut(s) 212, 489
XbaI TCTAGA 1 cut(s) 386
XspI CTAG 4 cut(s) 375, 387, 474, 530
ZraI GACGTC 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.