Rroxscaffold_1G00022390

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001571
Physical Location & Seq
Forward (+)
27630706 .. 27638137
7432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022390.1

Sequence Viewer

Length: 1053 bp
ATGCTTACATACGAGCAAGACCCGGATGTTGTTCGGTGGGGACTCCAGCTCTTTGAAGGTGATCCGTATTCGAATTGTGGGTATTGTGACTCGGTTAACCAAGATGGCACTAATTATTATCCGGGGCATTATTTTAAGGAAGATGTGGTGTATGGTGCGGATTGTATAAATGTGGAATATGATGAACTTATTGCTCATGCTCTTCAAGAAGAGCTGTCACTACTTGATATAGAGGACGAGCCGGGATCTGCGCCTGAGCAAGCAGAGCATTTGCAAGCATCTGTCTTCCCACAAGATTGGTCACAGCAATCTATTGGAAATTATGGCTCTGGACAGGAGACTGTTCAGGCAGAGGCAGATGAAGTTGGGCCTTCTACTTCGTGTTCTAGCCCAGATGAAAAGTCGTACTATGTAGATGAGTGGTCATATTCCCTAGAGCTGGCTAATGAATATACATTTGATGGAGAGAGGTTGAACCAGATGGTTCCTGTCCCTCATGTTCCTAAAATTAATGGTGATATACCCTCGCTAGATCAAGCAACTTTAGATCACCAAAGGCTTCTGGACAGGTTGCAGTTATATGGGTTGGTTGAAAATAAGGTCCAAGGAGATGGAAACTGTCAGTTCCGTGCTCTAGCTGACCAATTTTATCGATCTCCTGAACACCACGAATTTGTGAGACAAGAAATTGTAAATCAGCTCAAGTCATACCCAGAGATATATGAAGGATATGTTCCCATGGCTTATGATGACTATTTGGAGAAGATGTCCAGGAGCGGTGAGTGGGGCGATCATGTTACTTTACAGGCTGCTGCAGATTTGTATGGAGTTAAAATTTTTGTCATAACATCTTTCAAGGACACCTGCTACATTGAAATTCTTCCTAATGCTGAAAGATCGAAACGAGTAATTTGTTTGAGCTTTTGGGCAGAGGTGCACTACAACTCAATTAATTCTGAAGGAGATTTGCCTACATTTGAGACGAAGAAGAAGAAGAAAAGGGGATGTCACGTAATAAGCACTTGGAGTCCCCAAATAAATACCAATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

350

Amino Acids

39.93

Weight (kDa)

4.43

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 202 - 308 2.8e-10 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 872
Acc36I ACCTGC 1 cut(s) 872
AccBSI CCGCTC 1 cut(s) 777
AciI CCGC 2 cut(s) 158, 777
AclWI GGATC 2 cut(s) 56, 253
AcsI RAATTY 3 cut(s) 671, 834, 876
AcuI CTGAAG 1 cut(s) 978
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 439
AgsI TTSAA 6 cut(s) 56, 206, 475, 593, 856, 875
AjnI CCWGG 1 cut(s) 770
AluBI AGCT 6 cut(s) 49, 214, 439, 638, 700, 921
AluI AGCT 6 cut(s) 49, 214, 439, 638, 700, 921
Alw21I GWGCWC 2 cut(s) 634, 939
Alw26I GTCTC 3 cut(s) 332, 673, 974
Alw44I GTGCAC 1 cut(s) 935
AlwI GGATC 2 cut(s) 56, 253
AoxI GGCC 1 cut(s) 368
ApaLI GTGCAC 1 cut(s) 935
ApeKI GCWGC 2 cut(s) 809, 812
ApoI RAATTY 3 cut(s) 671, 834, 876
AseI ATTAAT 2 cut(s) 510, 951
Asp700I GAANNNNTTC 1 cut(s) 879
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 2 cut(s) 368, 601
AsuC2I CCSGG 3 cut(s) 23, 123, 243
AsuHPI GGTGA 4 cut(s) 71, 527, 542, 791
AsuII TTCGAA 1 cut(s) 71
AvaII GGWCC 1 cut(s) 601
BaeGI GKGCMC 1 cut(s) 939
BbsI GAAGAC 1 cut(s) 277
Bbv12I GWGCWC 2 cut(s) 634, 939
BbvI GCAGC 2 cut(s) 796, 799
BccI CCATC 4 cut(s) 98, 455, 475, 605
BciT130I CCWGG 1 cut(s) 772
BcnI CCSGG 3 cut(s) 23, 123, 243
BcoDI GTCTC 3 cut(s) 332, 673, 974
BfaI CTAG 4 cut(s) 387, 434, 530, 635
BfmI CTRYAG 1 cut(s) 813
BfuAI ACCTGC 1 cut(s) 872
BisI GCNGC 2 cut(s) 810, 813
BlsI GCNGC 2 cut(s) 811, 814
Bme1390I CCNGG 4 cut(s) 23, 123, 243, 772
Bme18I GGWCC 1 cut(s) 601
BmgT120I GGNCC 2 cut(s) 368, 601
BmiI GGNNCC 1 cut(s) 486
BmrFI CCNGG 4 cut(s) 23, 123, 243, 772
BmsI GCATC 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 277
BpmI CTGGAG 1 cut(s) 29
Bpu10I CCTNAGC 1 cut(s) 255
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 3 cut(s) 23, 123, 243
Bsa29I ATCGAT 1 cut(s) 652
BsaAI YACGTR 1 cut(s) 1012
BsaJI CCNNGG 3 cut(s) 122, 604, 738
BsaXI ACNNNNNCTCC 2 cut(s) 752, 782
Bsc4I CCNNNNNNNGG 1 cut(s) 439
BseBI CCWGG 1 cut(s) 772
BseCI ATCGAT 1 cut(s) 652
BseDI CCNNGG 3 cut(s) 122, 604, 738
BseGI GGATG 2 cut(s) 31, 1010
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMII CTCAG 1 cut(s) 246
BseSI GKGCMC 1 cut(s) 939
BseXI GCAGC 2 cut(s) 796, 799
BshFI GGCC 1 cut(s) 370
BshVI ATCGAT 1 cut(s) 652
BsiHKAI GWGCWC 2 cut(s) 634, 939
BsiSI CCGG 3 cut(s) 23, 122, 242
BslFI GGGAC 3 cut(s) 54, 476, 1014
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 3 cut(s) 332, 673, 974
BsmBI CGTCTC 1 cut(s) 974
BsmFI GGGAC 3 cut(s) 54, 476, 1014
BsnI GGCC 1 cut(s) 370
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 634, 939
Bsp143I GATC 7 cut(s) 61, 245, 532, 547, 653, 790, 896
Bsp19I CCATGG 1 cut(s) 738
BspACI CCGC 2 cut(s) 158, 777
BspANI GGCC 1 cut(s) 370
BspCNI CTCAG 1 cut(s) 247
BspDI ATCGAT 1 cut(s) 652
BspLI GGNNCC 1 cut(s) 486
BspMAI CTGCAG 1 cut(s) 817
BspMI ACCTGC 1 cut(s) 872
BspPI GGATC 2 cut(s) 56, 253
BspQI GCTCTTC 2 cut(s) 204, 207
BspT104I TTCGAA 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 777
BssECI CCNNGG 3 cut(s) 122, 604, 738
BssMI GATC 7 cut(s) 61, 245, 532, 547, 653, 790, 896
BssT1I CCWWGG 2 cut(s) 604, 738
Bst2UI CCWGG 1 cut(s) 772
Bst4CI ACNGT 2 cut(s) 343, 620
Bst6I CTCTTC 2 cut(s) 204, 207
BstBAI YACGTR 1 cut(s) 1012
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 3 cut(s) 261, 276, 441
BstDEI CTNAG 1 cut(s) 255
BstDSI CCRYGG 1 cut(s) 738
BstF5I GGATG 2 cut(s) 31, 1010
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 7 cut(s) 64, 248, 535, 550, 656, 793, 899
BstMAI GTCTC 3 cut(s) 332, 673, 974
BstMBI GATC 7 cut(s) 61, 245, 532, 547, 653, 790, 896
BstMWI GCNNNNNNNGC 1 cut(s) 265
BstNI CCWGG 1 cut(s) 772
BstSCI CCNGG 4 cut(s) 21, 121, 241, 770
BstSFI CTRYAG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 939
BstV1I GCAGC 2 cut(s) 796, 799
BstV2I GAAGAC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 245
BstXI CCANNNNNNTGG 2 cut(s) 297, 611
BstYI RGATCY 1 cut(s) 245
Bsu15I ATCGAT 1 cut(s) 652
BsuRI GGCC 1 cut(s) 370
BsuTUI ATCGAT 1 cut(s) 652
BtgI CCRYGG 1 cut(s) 738
BtsCI GGATG 2 cut(s) 31, 1010
BveI ACCTGC 1 cut(s) 872
Cac8I GCNNGC 3 cut(s) 261, 276, 441
CfoI GCGC 1 cut(s) 253
Cfr13I GGNCC 2 cut(s) 368, 601
ClaI ATCGAT 1 cut(s) 652
Csp6I GTAC 1 cut(s) 406
CviAII CATG 4 cut(s) 197, 497, 739, 794
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 1 cut(s) 255
DpnI GATC 7 cut(s) 63, 247, 534, 549, 655, 792, 898
DpnII GATC 7 cut(s) 61, 245, 532, 547, 653, 790, 896
Eam1104I CTCTTC 2 cut(s) 204, 207
EarI CTCTTC 2 cut(s) 204, 207
Eco130I CCWWGG 2 cut(s) 604, 738
Eco47I GGWCC 1 cut(s) 601
Eco57I CTGAAG 1 cut(s) 978
EcoRII CCWGG 1 cut(s) 770
EcoT14I CCWWGG 2 cut(s) 604, 738
ErhI CCWWGG 2 cut(s) 604, 738
Esp3I CGTCTC 1 cut(s) 974
FaeI CATG 4 cut(s) 200, 500, 742, 797
FaqI GGGAC 3 cut(s) 54, 476, 1014
FatI CATG 4 cut(s) 196, 496, 738, 793
Fnu4HI GCNGC 2 cut(s) 810, 813
FokI GGATG 2 cut(s) 38, 1017
Fsp4HI GCNGC 2 cut(s) 810, 813
FspBI CTAG 4 cut(s) 387, 434, 530, 635
GlaI GCGC 1 cut(s) 252
GluI GCNGC 2 cut(s) 810, 813
GsuI CTGGAG 1 cut(s) 29
HaeIII GGCC 1 cut(s) 370
HapII CCGG 3 cut(s) 23, 122, 242
HhaI GCGC 1 cut(s) 253
Hin1II CATG 4 cut(s) 200, 500, 742, 797
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 3 cut(s) 42, 89, 1027
HpaI GTTAAC 1 cut(s) 97
HpaII CCGG 3 cut(s) 23, 122, 242
HphI GGTGA 4 cut(s) 71, 527, 542, 791
Hpy166II GTNNAC 2 cut(s) 97, 937
Hpy188I TCNGA 1 cut(s) 958
Hpy188III TCNNGA 4 cut(s) 206, 330, 563, 659
Hpy8I GTNNAC 2 cut(s) 97, 937
HpyAV CCTTC 4 cut(s) 50, 381, 719, 953
HpyCH4III ACNGT 2 cut(s) 343, 620
HpyCH4IV ACGT 1 cut(s) 1011
HpyCH4V TGCA 4 cut(s) 274, 574, 815, 937
HpyF10VI GCNNNNNNNGC 1 cut(s) 265
HpyF3I CTNAG 1 cut(s) 255
HpySE526I ACGT 1 cut(s) 1011
Hsp92II CATG 4 cut(s) 200, 500, 742, 797
HspAI GCGC 1 cut(s) 251
KspAI GTTAAC 1 cut(s) 97
Kzo9I GATC 7 cut(s) 61, 245, 532, 547, 653, 790, 896
LguI GCTCTTC 2 cut(s) 204, 207
LmnI GCTCC 1 cut(s) 774
Lsp1109I GCAGC 2 cut(s) 796, 799
LweI GCATC 1 cut(s) 287
MaeI CTAG 4 cut(s) 387, 434, 530, 635
MaeII ACGT 1 cut(s) 1011
MaeIII GTNAC 5 cut(s) 86, 216, 300, 796, 1007
MalI GATC 7 cut(s) 63, 247, 534, 549, 655, 792, 898
MbiI CCGCTC 1 cut(s) 777
MboI GATC 7 cut(s) 61, 245, 532, 547, 653, 790, 896
MflI RGATCY 1 cut(s) 245
MhlI GDGCHC 2 cut(s) 634, 939
MlyI GAGTC 3 cut(s) 36, 83, 1036
MnlI CCTC 6 cut(s) 226, 346, 462, 504, 535, 925
MroXI GAANNNNTTC 1 cut(s) 879
MseI TTAA 5 cut(s) 96, 135, 510, 831, 951
MspI CCGG 3 cut(s) 23, 122, 242
MspR9I CCNGG 4 cut(s) 23, 123, 243, 772
MvaI CCWGG 1 cut(s) 772
MwoI GCNNNNNNNGC 1 cut(s) 265
NciI CCSGG 3 cut(s) 23, 123, 243
NcoI CCATGG 1 cut(s) 738
NdeII GATC 7 cut(s) 61, 245, 532, 547, 653, 790, 896
NlaIII CATG 4 cut(s) 200, 500, 742, 797
NlaIV GGNNCC 1 cut(s) 486
NmuCI GTSAC 4 cut(s) 86, 216, 300, 1007
NspV TTCGAA 1 cut(s) 71
PaqCI CACCTGC 1 cut(s) 872
PciSI GCTCTTC 2 cut(s) 204, 207
PdmI GAANNNNTTC 1 cut(s) 879
PfoI TCCNGGA 1 cut(s) 770
PkrI GCNGC 2 cut(s) 811, 814
PleI GAGTC 3 cut(s) 36, 83, 1035
PpsI GAGTC 3 cut(s) 36, 83, 1035
Ppu21I YACGTR 1 cut(s) 1012
PshBI ATTAAT 2 cut(s) 510, 951
Psp6I CCWGG 1 cut(s) 770
PspGI CCWGG 1 cut(s) 770
PspN4I GGNNCC 1 cut(s) 486
PspPI GGNCC 2 cut(s) 368, 601
PstI CTGCAG 1 cut(s) 817
PsuI RGATCY 1 cut(s) 245
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
SapI GCTCTTC 2 cut(s) 204, 207
SaqAI TTAA 5 cut(s) 96, 135, 510, 831, 951
SatI GCNGC 2 cut(s) 810, 813
Sau3AI GATC 7 cut(s) 61, 245, 532, 547, 653, 790, 896
Sau96I GGNCC 2 cut(s) 368, 601
SchI GAGTC 3 cut(s) 36, 83, 1036
ScrFI CCNGG 4 cut(s) 23, 123, 243, 772
SduI GDGCHC 2 cut(s) 634, 939
SfaNI GCATC 1 cut(s) 287
SfcI CTRYAG 1 cut(s) 813
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 1 cut(s) 601
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
SsiI CCGC 2 cut(s) 158, 777
SspMI CTAG 4 cut(s) 387, 434, 530, 635
StyD4I CCNGG 4 cut(s) 21, 121, 241, 770
StyI CCWWGG 2 cut(s) 604, 738
TaaI ACNGT 2 cut(s) 343, 620
TaiI ACGT 1 cut(s) 1014
TaqI TCGA 3 cut(s) 71, 652, 899
Tru1I TTAA 5 cut(s) 96, 135, 510, 831, 951
Tru9I TTAA 5 cut(s) 96, 135, 510, 831, 951
TseFI GTSAC 4 cut(s) 86, 216, 300, 1007
TseI GCWGC 2 cut(s) 809, 812
Tsp45I GTSAC 4 cut(s) 86, 216, 300, 1007
TspDTI ATGAA 5 cut(s) 198, 375, 411, 462, 738
TspGWI ACGGA 2 cut(s) 54, 617
VneI GTGCAC 1 cut(s) 935
VpaK11BI GGWCC 1 cut(s) 601
VspI ATTAAT 2 cut(s) 510, 951
XapI RAATTY 3 cut(s) 671, 834, 876
XmnI GAANNNNTTC 1 cut(s) 879
XspI CTAG 4 cut(s) 387, 434, 530, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.