MD04G1095000.v1.1

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001571
Physical Location & Seq
Reverse (-)
17425835 .. 17431887
6053 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1095000.v1.1.491

Sequence Viewer

Length: 999 bp
ATGGCTTCCAGTGAGTATGATTCAGATATTGTTCAGTGGGGTCTTCGTCTTCTTGATGGGGATCCAGTTTATAATTCTTCATACTACTACGGCAGTGACATGATACAACAAGATGCTAATGATATCTACCATTATGAGCATATCAGGAGTCATTATGATACAGAATCAAACAATGTAGAAAATGATGAAATCATTGCACATACTCTTCAAGAGGAATATTCGCGACTATCTGTAACAGAAGCCTATGAATACTCGCATGCAGGTGAAGCACAGCCACAAGCTTCGTTTCATGCAGATGTGTGGCATGGTCAATCTACAAGAGATTTTTGTTCAGACCATGATTACAGCCAGGAAGAAGCTGCTATTGTGGTGCCTTCTAGCTTATGTTCTAGCCCGGATGATCAATTGGAGCTAACTGATGAATGTTCTCTTCATGGGGAAGTAGGCTGGGGCTGGAATCCTGTTCCCCACATTCCTAGAATCAATGGAGAAATTCCTTCAATTGATGAAGCGACTTCAGACCATCAAAGGCTAGTTGACAGGCTTCAATTATATGGCTTTGTTGAACATAAGGTAGCAGGAGATGGCAACTGCCAGTTCCATGCTTTATCAGACCAATTGTATCAATCACCTGACAACCACGAGTATGTGAGAAAGCAAGTCGTAAATCAGCTTAAGTCTCATCCAGAGATTTACGAGGGATATGTTCCTATGGCATATGATGATTATTTGGAGAAGATGTCCAAGGATGGTGAGTGGGGTGACCATGTCTCATTACAGGCAGCTGCTGATTCGTACGGAGTGAAAATTTTTGTCCTGACTTCTTTTAAGGATAACTGTTGCATAGAGATTCTTCCTAATATTCAATCTTCAAAACAAGTTATTTATTTGAGTTTCTGGGCAGAGGTGCATTACAACTCAATTTATCCTCAAGGAGGCACAACCTCATATGAGAACAGAAAAAAGAAAAAATGGCGGATATTTGGGAATAAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

333

Amino Acids

38.1

Weight (kDa)

4.73

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 194 - 280 1.2e-13 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 72
AarI CACCTGC 1 cut(s) 251
Acc36I ACCTGC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 370
AccII CGCG 1 cut(s) 223
AciI CCGC 1 cut(s) 976
AclWI GGATC 2 cut(s) 56, 69
AcsI RAATTY 2 cut(s) 492, 807
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 1 cut(s) 797
AfiI CCNNNNNNNGG 1 cut(s) 935
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 6 cut(s) 209, 501, 548, 566, 866, 873
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 6 cut(s) 281, 359, 381, 412, 673, 785
AluI AGCT 6 cut(s) 281, 359, 381, 412, 673, 785
Alw26I GTCTC 2 cut(s) 684, 775
AlwI GGATC 2 cut(s) 56, 69
AlwNI CAGNNNCTG 1 cut(s) 788
ApeKI GCWGC 3 cut(s) 359, 782, 785
ApoI RAATTY 2 cut(s) 492, 807
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 4 cut(s) 275, 621, 764, 773
BamHI GGATCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 370
BauI CACGAG 1 cut(s) 641
BbsI GAAGAC 2 cut(s) 35, 41
BbvI GCAGC 3 cut(s) 346, 772, 794
BccI CCATC 4 cut(s) 50, 531, 578, 743
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 350
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 2 cut(s) 684, 775
BfaI CTAG 4 cut(s) 378, 390, 477, 533
BfrI CTTAAG 1 cut(s) 674
BfuAI ACCTGC 1 cut(s) 251
BisI GCNGC 3 cut(s) 360, 783, 786
BlsI GCNGC 3 cut(s) 361, 784, 787
Bme1390I CCNGG 2 cut(s) 350, 395
BmiI GGNNCC 2 cut(s) 63, 372
BmrFI CCNGG 2 cut(s) 350, 395
BmsI GCATC 1 cut(s) 103
BpiI GAAGAC 2 cut(s) 35, 41
BpuEI CTTGAG 1 cut(s) 915
BpuMI CCSGG 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 744
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bsc4I CCNNNNNNNGG 1 cut(s) 935
Bse1I ACTGG 3 cut(s) 9, 65, 595
Bse3DI GCAATG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 60
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 744
BseGI GGATG 3 cut(s) 403, 682, 754
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 1 cut(s) 935
BseMI GCAATG 1 cut(s) 192
BseNI ACTGG 3 cut(s) 9, 65, 595
BseXI GCAGC 3 cut(s) 346, 772, 794
BseYI CCCAGC 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 223
BshNI GGYRCC 1 cut(s) 370
BsiSI CCGG 1 cut(s) 395
BsiWI CGTACG 1 cut(s) 795
BslI CCNNNNNNNGG 1 cut(s) 935
BsmAI GTCTC 2 cut(s) 684, 775
Bsp143I GATC 2 cut(s) 61, 400
Bsp68I TCGCGA 1 cut(s) 223
BspACI CCGC 1 cut(s) 976
BspFNI CGCG 1 cut(s) 223
BspLI GGNNCC 2 cut(s) 63, 372
BspMI ACCTGC 1 cut(s) 251
BspPI GGATC 2 cut(s) 56, 69
BspT107I GGYRCC 1 cut(s) 370
BspTI CTTAAG 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 192
BsrI ACTGG 3 cut(s) 9, 65, 595
BssECI CCNNGG 1 cut(s) 744
BssMI GATC 2 cut(s) 61, 400
BssSI CACGAG 1 cut(s) 641
BssT1I CCWWGG 1 cut(s) 744
Bst2BI CACGAG 1 cut(s) 641
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 1 cut(s) 839
Bst6I CTCTTC 2 cut(s) 210, 435
BstAFI CTTAAG 1 cut(s) 674
BstC8I GCNNGC 1 cut(s) 258
BstEII GGTNACC 1 cut(s) 761
BstENI CCTNNNNNAGG 1 cut(s) 933
BstF5I GGATG 3 cut(s) 403, 682, 754
BstFNI CGCG 1 cut(s) 223
BstKTI GATC 2 cut(s) 64, 403
BstMAI GTCTC 2 cut(s) 684, 775
BstMBI GATC 2 cut(s) 61, 400
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstNI CCWGG 1 cut(s) 350
BstNSI RCATGY 1 cut(s) 260
BstPI GGTNACC 1 cut(s) 761
BstSCI CCNGG 2 cut(s) 348, 393
BstUI CGCG 1 cut(s) 223
BstV1I GCAGC 3 cut(s) 346, 772, 794
BstV2I GAAGAC 2 cut(s) 35, 41
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
BtsCI GGATG 3 cut(s) 403, 682, 754
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 3 cut(s) 16, 41, 100
BtuMI TCGCGA 1 cut(s) 223
BveI ACCTGC 1 cut(s) 251
Cac8I GCNNGC 1 cut(s) 258
CaiI CAGNNNCTG 1 cut(s) 788
Csp6I GTAC 1 cut(s) 796
CviAII CATG 8 cut(s) 100, 257, 290, 305, 338, 434, 602, 767
CviQI GTAC 1 cut(s) 796
DpnI GATC 2 cut(s) 63, 402
DpnII GATC 2 cut(s) 61, 400
Eam1104I CTCTTC 2 cut(s) 210, 435
EarI CTCTTC 2 cut(s) 210, 435
EciI GGCGGA 1 cut(s) 991
Eco130I CCWWGG 1 cut(s) 744
Eco32I GATATC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 501
Eco91I GGTNACC 1 cut(s) 761
EcoNI CCTNNNNNAGG 1 cut(s) 933
EcoO65I GGTNACC 1 cut(s) 761
EcoRII CCWGG 1 cut(s) 348
EcoRV GATATC 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 744
ErhI CCWWGG 1 cut(s) 744
FaeI CATG 8 cut(s) 103, 260, 293, 308, 341, 437, 605, 770
FatI CATG 8 cut(s) 99, 256, 289, 304, 337, 433, 601, 766
FauNDI CATATG 2 cut(s) 718, 949
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 3 cut(s) 360, 783, 786
FokI GGATG 3 cut(s) 410, 669, 761
Fsp4HI GCNGC 3 cut(s) 360, 783, 786
FspBI CTAG 4 cut(s) 378, 390, 477, 533
GluI GCNGC 3 cut(s) 360, 783, 786
GsaI CCCAGC 1 cut(s) 451
HapII CCGG 1 cut(s) 395
Hin1II CATG 8 cut(s) 103, 260, 293, 308, 341, 437, 605, 770
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 7 cut(s) 20, 148, 164, 457, 480, 791, 850
HpaII CCGG 1 cut(s) 395
HphI GGTGA 4 cut(s) 275, 621, 764, 773
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 4 cut(s) 25, 334, 520, 613
Hpy188III TCNNGA 6 cut(s) 53, 145, 209, 222, 686, 817
Hpy8I GTNNAC 1 cut(s) 538
HpyAV CCTTC 2 cut(s) 384, 507
HpyCH4III ACNGT 1 cut(s) 839
HpyCH4V TGCA 5 cut(s) 197, 260, 293, 843, 910
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
Hsp92II CATG 8 cut(s) 103, 260, 293, 308, 341, 437, 605, 770
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 2 cut(s) 61, 400
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 3 cut(s) 346, 772, 794
LweI GCATC 1 cut(s) 103
MaeI CTAG 4 cut(s) 378, 390, 477, 533
MaeIII GTNAC 3 cut(s) 95, 232, 761
MalI GATC 2 cut(s) 63, 402
MboI GATC 2 cut(s) 61, 400
MboII GAAGA 9 cut(s) 35, 41, 69, 197, 365, 422, 748, 845, 861
MfeI CAATTG 3 cut(s) 404, 501, 617
MflI RGATCY 1 cut(s) 61
MluCI AATT 8 cut(s) 73, 404, 492, 501, 548, 617, 807, 921
MlyI GAGTC 1 cut(s) 157
MnlI CCTC 6 cut(s) 205, 691, 898, 929, 939, 955
MseI TTAA 2 cut(s) 675, 828
MslI CAYNNNNRTG 3 cut(s) 261, 294, 645
MspA1I CMGCKG 1 cut(s) 785
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 350, 395
MunI CAATTG 3 cut(s) 404, 501, 617
MvaI CCWGG 1 cut(s) 350
MvnI CGCG 1 cut(s) 223
MwoI GCNNNNNNNGC 1 cut(s) 266
NciI CCSGG 1 cut(s) 395
NdeI CATATG 2 cut(s) 718, 949
NdeII GATC 2 cut(s) 61, 400
NlaIII CATG 8 cut(s) 103, 260, 293, 308, 341, 437, 605, 770
NlaIV GGNNCC 2 cut(s) 63, 372
NmuCI GTSAC 2 cut(s) 95, 761
NruI TCGCGA 1 cut(s) 223
NspI RCATGY 1 cut(s) 260
PaeI GCATGC 1 cut(s) 260
PaqCI CACCTGC 1 cut(s) 251
PfeI GAWTC 6 cut(s) 20, 164, 457, 480, 791, 850
Pfl23II CGTACG 1 cut(s) 795
PflFI GACNNNGTC 1 cut(s) 767
PkrI GCNGC 3 cut(s) 361, 784, 787
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
PsiI TTATAA 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 348
PspEI GGTNACC 1 cut(s) 761
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 1 cut(s) 348
PspLI CGTACG 1 cut(s) 795
PspN4I GGNNCC 2 cut(s) 63, 372
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 1 cut(s) 61
PsyI GACNNNGTC 1 cut(s) 767
PvuII CAGCTG 1 cut(s) 785
RruI TCGCGA 1 cut(s) 223
RsaI GTAC 1 cut(s) 797
RsaNI GTAC 1 cut(s) 796
RseI CAYNNNNRTG 3 cut(s) 261, 294, 645
SaqAI TTAA 2 cut(s) 675, 828
SatI GCNGC 3 cut(s) 360, 783, 786
Sau3AI GATC 2 cut(s) 61, 400
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 2 cut(s) 350, 395
SfaNI GCATC 1 cut(s) 103
SmiMI CAYNNNNRTG 3 cut(s) 261, 294, 645
SmlI CTYRAG 2 cut(s) 674, 930
SmoI CTYRAG 2 cut(s) 674, 930
SphI GCATGC 1 cut(s) 260
Sse9I AATT 8 cut(s) 73, 404, 492, 501, 548, 617, 807, 921
SsiI CCGC 1 cut(s) 976
SspI AATATT 2 cut(s) 218, 862
SspMI CTAG 4 cut(s) 378, 390, 477, 533
StyD4I CCNGG 2 cut(s) 348, 393
StyI CCWWGG 1 cut(s) 744
TaaI ACNGT 1 cut(s) 839
TasI AATT 8 cut(s) 73, 404, 492, 501, 548, 617, 807, 921
TfiI GAWTC 6 cut(s) 20, 164, 457, 480, 791, 850
Tru1I TTAA 2 cut(s) 675, 828
Tru9I TTAA 2 cut(s) 675, 828
TscAI CASTG 3 cut(s) 16, 41, 100
TseFI GTSAC 2 cut(s) 95, 761
TseI GCWGC 3 cut(s) 359, 782, 785
Tsp45I GTSAC 2 cut(s) 95, 761
TspDTI ATGAA 7 cut(s) 69, 201, 261, 278, 422, 435, 522
TspGWI ACGGA 1 cut(s) 813
TspRI CASTG 3 cut(s) 16, 41, 100
Tth111I GACNNNGTC 1 cut(s) 767
Vha464I CTTAAG 1 cut(s) 674
XagI CCTNNNNNAGG 1 cut(s) 933
XapI RAATTY 2 cut(s) 492, 807
XceI RCATGY 1 cut(s) 260
XspI CTAG 4 cut(s) 378, 390, 477, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.