Rmu_sc0009972.1_g000001

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009972.1
OG0001571
Physical Location & Seq
Reverse (-)
1 .. 1792
1792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009972.1_g000001.1.cds

Sequence Viewer

Length: 897 bp
atggctggccaagaacatgagtctggtattatgcagtgggctcttagtctacttgatggcgatccagcttataatactgtctataatggtgacatgatacaacacgatgctaatgacatctaccacggacaatattttagaggtcaatatggtactgaatctaaccatgtagagaatgatgagatcattgcacacacacttcaagaagaatattcacgactatctgtagcagaagcctctcgatactcacatggagatgaagagcaatcacaagcatctactcatgcagatgattggtatagtttgtccagaaggaactattgttcagacagtgattatccaaatgaaggagcatgcacagtggtgccttctagttcatgttctagcccaggttgtttggagttaacagatgagtgtgcagttgacggtgaagtaggctggagcttcaatgaaatgatgcccgttcctgtaacaggcggcgacggcggcgcggggcttgcaggagggaggccggaggcgtcccggaccgttctaggcaccgttcaaggtcggaggaggtcaggcttgccggtttctgggcagccacctcacctgcaactgcaggttctgtgcagcaccgcaaaacccattgccggagactccacccgaccgcagacccctgacctccctccagcaagccgcgccgctccgtcgccgctcgatcgaggccggagcagtgacgtccgcactttttatgggttgcggacatccgctctcgaactgctccgtcatattcctagaattaatggagaaattccttcagttgatgaagctacttcagatcatcagaggctgcttgacagactgcaattatatggcgtcgtggagcgtaaggttcaaggagatggtaactgtcag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

299

Amino Acids

32.48

Weight (kDa)

4.8

Isoelectric Point (pI)

58.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 72
AarI CACCTGC 1 cut(s) 600
AatII GACGTC 1 cut(s) 723
Acc36I ACCTGC 2 cut(s) 592, 600
AccB1I GGYRCC 2 cut(s) 364, 536
AccBSI CCGCTC 3 cut(s) 686, 697, 752
AccI GTMKAC 1 cut(s) 49
AccII CGCG 2 cut(s) 491, 681
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 7
AcsI RAATTY 1 cut(s) 792
AcuI CTGAAG 2 cut(s) 783, 801
AcyI GRCGYC 3 cut(s) 518, 720, 858
AfaI GTAC 1 cut(s) 154
AfiI CCNNNNNNNGG 4 cut(s) 347, 473, 575, 632
AgsI TTSAA 4 cut(s) 203, 448, 545, 878
AjnI CCWGG 1 cut(s) 388
AleI CACNNNNGTG 1 cut(s) 362
AloI GAACNNNNNNTCC 2 cut(s) 307, 339
AluBI AGCT 3 cut(s) 68, 444, 812
AluI AGCT 3 cut(s) 68, 444, 812
Alw26I GTCTC 1 cut(s) 630
AlwI GGATC 1 cut(s) 56
AlwNI CAGNNNCTG 2 cut(s) 607, 832
AoxI GGCC 3 cut(s) 7, 509, 706
ApeKI GCWGC 3 cut(s) 580, 612, 832
ApoI RAATTY 1 cut(s) 792
AseI ATTAAT 1 cut(s) 783
AspLEI GCGC 2 cut(s) 491, 683
AspS9I GGNCC 1 cut(s) 525
AsuC2I CCSGG 1 cut(s) 523
AsuHPI GGTGA 3 cut(s) 101, 440, 581
AvaII GGWCC 1 cut(s) 525
BalI TGGCCA 1 cut(s) 9
BanI GGYRCC 2 cut(s) 364, 536
BanII GRGCYC 1 cut(s) 43
BbvI GCAGC 3 cut(s) 592, 624, 819
BccI CCATC 2 cut(s) 50, 878
BceAI ACGGC 1 cut(s) 499
BciT130I CCWGG 1 cut(s) 390
BcnI CCSGG 1 cut(s) 523
BcoDI GTCTC 1 cut(s) 630
BfaI CTAG 4 cut(s) 372, 384, 533, 777
BfmI CTRYAG 2 cut(s) 225, 599
BfuAI ACCTGC 2 cut(s) 592, 600
BisI GCNGC 8 cut(s) 478, 487, 581, 613, 679, 684, 695, 833
BlsI GCNGC 8 cut(s) 479, 488, 582, 614, 680, 685, 696, 834
Bme1390I CCNGG 2 cut(s) 390, 523
Bme18I GGWCC 1 cut(s) 525
BmgT120I GGNCC 1 cut(s) 525
BmiI GGNNCC 2 cut(s) 366, 538
BmrFI CCNGG 2 cut(s) 390, 523
BmsI GCATC 3 cut(s) 97, 284, 447
BpmI CTGGAG 2 cut(s) 460, 654
BpuMI CCSGG 1 cut(s) 523
BsaBI GATNNNNATC 1 cut(s) 60
BsaHI GRCGYC 3 cut(s) 518, 720, 858
BsaJI CCNNGG 2 cut(s) 124, 388
Bsc4I CCNNNNNNNGG 4 cut(s) 347, 473, 575, 632
Bse118I RCCGGY 1 cut(s) 568
Bse3DI GCAATG 2 cut(s) 186, 627
Bse8I GATNNNNATC 1 cut(s) 60
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 2 cut(s) 124, 388
BseGI GGATG 1 cut(s) 746
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 4 cut(s) 347, 473, 575, 632
BseMI GCAATG 2 cut(s) 186, 627
BseRI GAGGAG 1 cut(s) 568
BseXI GCAGC 3 cut(s) 592, 624, 819
BsgI GTGCAG 2 cut(s) 438, 631
Bsh1236I CGCG 2 cut(s) 491, 681
Bsh1285I CGRYCG 2 cut(s) 650, 703
BshFI GGCC 3 cut(s) 9, 511, 708
BshNI GGYRCC 2 cut(s) 364, 536
BsiEI CGRYCG 2 cut(s) 650, 703
BsiSI CCGG 5 cut(s) 512, 523, 569, 633, 709
BslFI GGGAC 1 cut(s) 505
BslI CCNNNNNNNGG 4 cut(s) 347, 473, 575, 632
BsmAI GTCTC 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 505
BsnI GGCC 3 cut(s) 9, 511, 708
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 4 cut(s) 61, 183, 700, 820
BspANI GGCC 3 cut(s) 9, 511, 708
BspFNI CGCG 2 cut(s) 491, 681
BspLI GGNNCC 2 cut(s) 366, 538
BspMAI CTGCAG 1 cut(s) 603
BspMI ACCTGC 2 cut(s) 592, 600
BspPI GGATC 1 cut(s) 56
BspQI GCTCTTC 1 cut(s) 255
BspT107I GGYRCC 2 cut(s) 364, 536
BsrBI CCGCTC 3 cut(s) 686, 697, 752
BsrDI GCAATG 2 cut(s) 186, 627
BsrFI RCCGGY 1 cut(s) 568
BssAI RCCGGY 1 cut(s) 568
BssECI CCNNGG 2 cut(s) 124, 388
BssMI GATC 4 cut(s) 61, 183, 700, 820
BssNI GRCGYC 3 cut(s) 518, 720, 858
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 7 cut(s) 79, 332, 361, 428, 529, 541, 893
Bst6I CTCTTC 1 cut(s) 255
BstACI GRCGYC 3 cut(s) 518, 720, 858
BstC8I GCNNGC 5 cut(s) 7, 355, 498, 566, 676
BstDEI CTNAG 1 cut(s) 44
BstDSI CCRYGG 1 cut(s) 124
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 1 cut(s) 746
BstFNI CGCG 2 cut(s) 491, 681
BstHHI GCGC 2 cut(s) 491, 683
BstKTI GATC 4 cut(s) 64, 186, 703, 823
BstMAI GTCTC 1 cut(s) 630
BstMBI GATC 4 cut(s) 61, 183, 700, 820
BstMCI CGRYCG 2 cut(s) 650, 703
BstMWI GCNNNNNNNGC 4 cut(s) 483, 486, 497, 680
BstNI CCWGG 1 cut(s) 390
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 388, 521
BstSFI CTRYAG 2 cut(s) 225, 599
BstUI CGCG 2 cut(s) 491, 681
BstV1I GCAGC 3 cut(s) 592, 624, 819
BsuRI GGCC 3 cut(s) 9, 511, 708
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 1 cut(s) 746
BtsI GCAGTG 2 cut(s) 41, 721
BtsIMutI CAGTG 4 cut(s) 41, 337, 366, 721
BveI ACCTGC 2 cut(s) 592, 600
Cac8I GCNNGC 5 cut(s) 7, 355, 498, 566, 676
CaiI CAGNNNCTG 2 cut(s) 607, 832
CfoI GCGC 2 cut(s) 491, 683
Cfr10I RCCGGY 1 cut(s) 568
Cfr13I GGNCC 1 cut(s) 525
CpoI CGGWCCG 1 cut(s) 525
CseI GACGC 2 cut(s) 507, 847
Csp6I GTAC 1 cut(s) 153
CspI CGGWCCG 1 cut(s) 525
CviAII CATG 7 cut(s) 17, 94, 167, 251, 284, 354, 378
CviQI GTAC 1 cut(s) 153
DdeI CTNAG 1 cut(s) 44
DpnI GATC 4 cut(s) 63, 185, 702, 822
DpnII GATC 4 cut(s) 61, 183, 700, 820
EaeI YGGCCR 1 cut(s) 7
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 525
Eco57I CTGAAG 2 cut(s) 783, 801
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoRII CCWGG 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 43
FaeI CATG 7 cut(s) 20, 97, 170, 254, 287, 357, 381
FaqI GGGAC 1 cut(s) 505
FatI CATG 7 cut(s) 16, 93, 166, 250, 283, 353, 377
FauI CCCGC 1 cut(s) 484
FblI GTMKAC 1 cut(s) 49
Fnu4HI GCNGC 8 cut(s) 478, 487, 581, 613, 679, 684, 695, 833
FokI GGATG 1 cut(s) 733
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 8 cut(s) 478, 487, 581, 613, 679, 684, 695, 833
FspBI CTAG 4 cut(s) 372, 384, 533, 777
GlaI GCGC 2 cut(s) 490, 682
GluI GCNGC 8 cut(s) 478, 487, 581, 613, 679, 684, 695, 833
GsuI CTGGAG 2 cut(s) 460, 654
HaeIII GGCC 3 cut(s) 9, 511, 708
HapII CCGG 5 cut(s) 512, 523, 569, 633, 709
HgaI GACGC 2 cut(s) 507, 847
HhaI GCGC 2 cut(s) 491, 683
Hin1I GRCGYC 3 cut(s) 518, 720, 858
Hin1II CATG 7 cut(s) 20, 97, 170, 254, 287, 357, 381
Hin6I GCGC 2 cut(s) 489, 681
HinP1I GCGC 2 cut(s) 489, 681
HincII GTYRAC 2 cut(s) 405, 424
HindII GTYRAC 2 cut(s) 405, 424
HinfI GANTC 3 cut(s) 20, 158, 638
HpaI GTTAAC 1 cut(s) 405
HpaII CCGG 5 cut(s) 512, 523, 569, 633, 709
HphI GGTGA 3 cut(s) 101, 440, 581
Hpy166II GTNNAC 3 cut(s) 50, 405, 424
Hpy188I TCNGA 4 cut(s) 328, 552, 820, 828
Hpy188III TCNNGA 5 cut(s) 203, 216, 240, 309, 755
Hpy8I GTNNAC 3 cut(s) 50, 405, 424
Hpy99I CGWCG 3 cut(s) 485, 694, 863
HpyAV CCTTC 4 cut(s) 306, 341, 378, 807
HpyCH4III ACNGT 7 cut(s) 79, 332, 361, 428, 529, 541, 893
HpyCH4IV ACGT 1 cut(s) 720
HpyF10VI GCNNNNNNNGC 4 cut(s) 483, 486, 497, 680
HpyF3I CTNAG 1 cut(s) 44
HpySE526I ACGT 1 cut(s) 720
Hsp92I GRCGYC 3 cut(s) 518, 720, 858
Hsp92II CATG 7 cut(s) 20, 97, 170, 254, 287, 357, 381
HspAI GCGC 2 cut(s) 489, 681
KspAI GTTAAC 1 cut(s) 405
Kzo9I GATC 4 cut(s) 61, 183, 700, 820
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 6 cut(s) 350, 441, 691, 711, 768, 865
Lsp1109I GCAGC 3 cut(s) 592, 624, 819
LweI GCATC 3 cut(s) 97, 284, 447
MaeI CTAG 4 cut(s) 372, 384, 533, 777
MaeII ACGT 1 cut(s) 720
MaeIII GTNAC 4 cut(s) 89, 469, 716, 887
MalI GATC 4 cut(s) 63, 185, 702, 822
MbiI CCGCTC 3 cut(s) 686, 697, 752
MboI GATC 4 cut(s) 61, 183, 700, 820
MboII GAAGA 2 cut(s) 218, 272
MhlI GDGCHC 1 cut(s) 43
MlsI TGGCCA 1 cut(s) 9
MluCI AATT 3 cut(s) 780, 792, 848
MluNI TGGCCA 1 cut(s) 9
MlyI GAGTC 2 cut(s) 29, 632
MmeI TCCRAC 1 cut(s) 530
Mox20I TGGCCA 1 cut(s) 9
MscI TGGCCA 1 cut(s) 9
MseI TTAA 2 cut(s) 404, 783
MslI CAYNNNNRTG 3 cut(s) 255, 288, 362
Msp20I TGGCCA 1 cut(s) 9
MspI CCGG 5 cut(s) 512, 523, 569, 633, 709
MspR9I CCNGG 2 cut(s) 390, 523
MvaI CCWGG 1 cut(s) 390
MvnI CGCG 2 cut(s) 491, 681
MwoI GCNNNNNNNGC 4 cut(s) 483, 486, 497, 680
NciI CCSGG 1 cut(s) 523
NdeII GATC 4 cut(s) 61, 183, 700, 820
NlaIII CATG 7 cut(s) 20, 97, 170, 254, 287, 357, 381
NlaIV GGNNCC 2 cut(s) 366, 538
NmuCI GTSAC 2 cut(s) 89, 716
NspI RCATGY 1 cut(s) 357
OliI CACNNNNGTG 1 cut(s) 362
PaeI GCATGC 1 cut(s) 357
PaqCI CACCTGC 1 cut(s) 600
PciSI GCTCTTC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 158
PfoI TCCNGGA 1 cut(s) 521
PkrI GCNGC 8 cut(s) 479, 488, 582, 614, 680, 685, 696, 834
Ple19I CGATCG 1 cut(s) 703
PleI GAGTC 2 cut(s) 28, 632
PpsI GAGTC 2 cut(s) 28, 632
PshBI ATTAAT 1 cut(s) 783
PsiI TTATAA 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 366, 538
PspPI GGNCC 1 cut(s) 525
PstI CTGCAG 1 cut(s) 603
PstNI CAGNNNCTG 2 cut(s) 607, 832
PvuI CGATCG 1 cut(s) 703
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
RseI CAYNNNNRTG 3 cut(s) 255, 288, 362
Rsr2I CGGWCCG 1 cut(s) 525
RsrII CGGWCCG 1 cut(s) 525
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 404, 783
SatI GCNGC 8 cut(s) 478, 487, 581, 613, 679, 684, 695, 833
Sau3AI GATC 4 cut(s) 61, 183, 700, 820
Sau96I GGNCC 1 cut(s) 525
SchI GAGTC 2 cut(s) 29, 632
ScrFI CCNGG 2 cut(s) 390, 523
SduI GDGCHC 1 cut(s) 43
SfaNI GCATC 3 cut(s) 97, 284, 447
SfcI CTRYAG 2 cut(s) 225, 599
SinI GGWCC 1 cut(s) 525
SmiMI CAYNNNNRTG 3 cut(s) 255, 288, 362
SphI GCATGC 1 cut(s) 357
Sse9I AATT 3 cut(s) 780, 792, 848
SspI AATATT 2 cut(s) 134, 212
SspMI CTAG 4 cut(s) 372, 384, 533, 777
StyD4I CCNGG 2 cut(s) 388, 521
TaaI ACNGT 7 cut(s) 79, 332, 361, 428, 529, 541, 893
TaiI ACGT 1 cut(s) 723
TaqI TCGA 4 cut(s) 241, 699, 703, 756
TasI AATT 3 cut(s) 780, 792, 848
TauI GCSGC 5 cut(s) 480, 489, 681, 686, 697
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 2 cut(s) 404, 783
Tru9I TTAA 2 cut(s) 404, 783
TscAI CASTG 4 cut(s) 41, 337, 366, 721
TseFI GTSAC 2 cut(s) 89, 716
TseI GCWGC 3 cut(s) 580, 612, 832
Tsp45I GTSAC 2 cut(s) 89, 716
TspDTI ATGAA 5 cut(s) 273, 360, 366, 465, 822
TspGWI ACGGA 3 cut(s) 141, 678, 755
TspRI CASTG 4 cut(s) 41, 337, 366, 721
VpaK11BI GGWCC 1 cut(s) 525
VspI ATTAAT 1 cut(s) 783
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 1 cut(s) 792
XceI RCATGY 1 cut(s) 357
XmiI GTMKAC 1 cut(s) 49
XspI CTAG 4 cut(s) 372, 384, 533, 777
ZraI GACGTC 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.