RchiOBHm_Chr5g0058171

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001571
Physical Location & Seq
Reverse (-)
62303038 .. 62310973
7936 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33487

Sequence Viewer

Length: 1059 bp
ATGCTTACATACGAGCAAGACCCGGATGTTGTTCGCTGGGGACTCCAGCTCTTTGAAGGTGATCCGTATTCGAATTGTGGGTATTGTGACTCAGTTAACCAAGATGGCACTAATTATTATGCGGGGCATTATTTTAAGGAAGATGTGGTGTATGATGCGGATTGTATAAATGTGGAATATGATGAACTTATTGCTCATGCTCTTCAAGAAGAGCTGTCACTACTTGATATAGAGGAGGACGAGCCGGGATCTGCGCCTGAGCAAGTAGAGCATTTGCAAGCATCTGTCTTCCCACAAGATTGGTCACAGCAATCTATTGGAAATTATGGCTCTGGACAGGAGAGTGTTCAGGCAGAGGCAGATGAAGTTGGGCCTTCTACTTCTACTTCGTGTTCTAGCCCAGATGAAAAGGCGTACTATGGAGATGAGTGGTCATATTCCCTAGAGCTGGCTAATGAATATACATTTGATGGAGACAGGTTGAACCAGATGGTTCCTGTCCCTCATGTTCCTAAAATTAATGGTGATATACCCTCGCTAGATCAAGCAACTTTAGATCACCAAAGGCTTCTGGACAGGTTGCAGTTATATGGGTTGGTTGAAAATAAGGTCCAAGGAGATGGAAACTGTCAGTTCCGCGCTCTAGCTGACCAATTTTATCAATCTCCTGAACACCATGAATTTGTGAGACAACAAATTGTAAATCAGCTCAAGTCATACCCAGAGATATATGAAGGATATGTTCCCATGGCTTATGATGACTATTTGGAGAAGATGTCCAGGAGCGGTGAGTGGGGCGATCATGTTACTTTACAGGCTGCTGCAGATTTGTATGGAGTTAAAATTTTTGTCATAACATCTTTCAAGGACACCTGCTACATTGAGATTCTTCCTAATGCTGAAAGATCGAAACGAGTAATTTGTTTGAGCTTTTGGGCAGAGGTGCACTACAACTCAATTTATTCTGAAGGAGATTTGCCTACATTTGAGACGAAGAAGAAGAAGAAAAGGGGGGTGTCACGTAATAAGCACTTGGAGTCCCCAAATAAATACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

352

Amino Acids

40.23

Weight (kDa)

4.45

Isoelectric Point (pI)

53.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 205 - 311 1.2e-10 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 881
Acc36I ACCTGC 1 cut(s) 881
AccBSI CCGCTC 1 cut(s) 786
AccII CGCG 1 cut(s) 639
AciI CCGC 4 cut(s) 122, 158, 637, 786
AclWI GGATC 2 cut(s) 56, 256
AcsI RAATTY 2 cut(s) 680, 843
AcuI CTGAAG 1 cut(s) 987
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 448
AgsI TTSAA 5 cut(s) 56, 206, 484, 602, 865
AjnI CCWGG 1 cut(s) 779
AluBI AGCT 6 cut(s) 49, 214, 448, 647, 709, 930
AluI AGCT 6 cut(s) 49, 214, 448, 647, 709, 930
Alw21I GWGCWC 1 cut(s) 948
Alw26I GTCTC 3 cut(s) 468, 682, 983
Alw44I GTGCAC 1 cut(s) 944
AlwI GGATC 2 cut(s) 56, 256
AoxI GGCC 1 cut(s) 371
ApaLI GTGCAC 1 cut(s) 944
ApeKI GCWGC 2 cut(s) 818, 821
ApoI RAATTY 2 cut(s) 680, 843
AseI ATTAAT 1 cut(s) 519
AspLEI GCGC 2 cut(s) 256, 641
AspS9I GGNCC 2 cut(s) 371, 610
AsuC2I CCSGG 2 cut(s) 23, 246
AsuHPI GGTGA 4 cut(s) 71, 536, 551, 800
AsuII TTCGAA 1 cut(s) 71
AvaII GGWCC 1 cut(s) 610
BaeGI GKGCMC 1 cut(s) 948
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 948
BbvI GCAGC 2 cut(s) 805, 808
BccI CCATC 4 cut(s) 98, 464, 484, 614
BciT130I CCWGG 1 cut(s) 781
BcnI CCSGG 2 cut(s) 23, 246
BcoDI GTCTC 3 cut(s) 468, 682, 983
BfaI CTAG 4 cut(s) 396, 443, 539, 644
BfmI CTRYAG 1 cut(s) 822
BfuAI ACCTGC 1 cut(s) 881
BisI GCNGC 2 cut(s) 819, 822
BlsI GCNGC 2 cut(s) 820, 823
Bme1390I CCNGG 3 cut(s) 23, 246, 781
Bme18I GGWCC 1 cut(s) 610
BmgT120I GGNCC 2 cut(s) 371, 610
BmiI GGNNCC 1 cut(s) 495
BmrFI CCNGG 3 cut(s) 23, 246, 781
BmsI GCATC 2 cut(s) 145, 290
BpiI GAAGAC 1 cut(s) 280
BpmI CTGGAG 1 cut(s) 29
Bpu10I CCTNAGC 1 cut(s) 258
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 695
BpuMI CCSGG 2 cut(s) 23, 246
BsaAI YACGTR 1 cut(s) 1022
BsaJI CCNNGG 2 cut(s) 613, 747
BsaXI ACNNNNNCTCC 2 cut(s) 761, 791
Bsc4I CCNNNNNNNGG 1 cut(s) 448
BseBI CCWGG 1 cut(s) 781
BseDI CCNNGG 2 cut(s) 613, 747
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 1 cut(s) 448
BseMII CTCAG 2 cut(s) 105, 249
BseRI GAGGAG 1 cut(s) 248
BseSI GKGCMC 1 cut(s) 948
BseXI GCAGC 2 cut(s) 805, 808
BseYI CCCAGC 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 639
BshFI GGCC 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 948
BsiSI CCGG 2 cut(s) 23, 245
BslFI GGGAC 3 cut(s) 54, 485, 1024
BslI CCNNNNNNNGG 1 cut(s) 448
BsmAI GTCTC 3 cut(s) 468, 682, 983
BsmBI CGTCTC 1 cut(s) 983
BsmFI GGGAC 3 cut(s) 54, 485, 1024
BsnI GGCC 1 cut(s) 373
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 948
Bsp143I GATC 6 cut(s) 61, 248, 541, 556, 799, 905
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 4 cut(s) 122, 158, 637, 786
BspANI GGCC 1 cut(s) 373
BspCNI CTCAG 2 cut(s) 104, 250
BspFNI CGCG 1 cut(s) 639
BspLI GGNNCC 1 cut(s) 495
BspMAI CTGCAG 1 cut(s) 826
BspMI ACCTGC 1 cut(s) 881
BspPI GGATC 2 cut(s) 56, 256
BspQI GCTCTTC 2 cut(s) 204, 207
BspT104I TTCGAA 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 786
BssECI CCNNGG 2 cut(s) 613, 747
BssMI GATC 6 cut(s) 61, 248, 541, 556, 799, 905
BssT1I CCWWGG 2 cut(s) 613, 747
Bst2UI CCWGG 1 cut(s) 781
Bst4CI ACNGT 1 cut(s) 629
Bst6I CTCTTC 2 cut(s) 204, 207
BstBAI YACGTR 1 cut(s) 1022
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 279, 450
BstDEI CTNAG 2 cut(s) 91, 258
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 1 cut(s) 31
BstFNI CGCG 1 cut(s) 639
BstHHI GCGC 2 cut(s) 256, 641
BstKTI GATC 6 cut(s) 64, 251, 544, 559, 802, 908
BstMAI GTCTC 3 cut(s) 468, 682, 983
BstMBI GATC 6 cut(s) 61, 248, 541, 556, 799, 905
BstMWI GCNNNNNNNGC 1 cut(s) 268
BstNI CCWGG 1 cut(s) 781
BstSCI CCNGG 3 cut(s) 21, 244, 779
BstSFI CTRYAG 1 cut(s) 822
BstSLI GKGCMC 1 cut(s) 948
BstUI CGCG 1 cut(s) 639
BstV1I GCAGC 2 cut(s) 805, 808
BstV2I GAAGAC 1 cut(s) 280
BstX2I RGATCY 1 cut(s) 248
BstXI CCANNNNNNTGG 2 cut(s) 300, 620
BstYI RGATCY 1 cut(s) 248
BsuRI GGCC 1 cut(s) 373
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 1 cut(s) 31
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 2 cut(s) 279, 450
CfoI GCGC 2 cut(s) 256, 641
Cfr13I GGNCC 2 cut(s) 371, 610
Csp6I GTAC 1 cut(s) 415
CviAII CATG 5 cut(s) 197, 506, 677, 748, 803
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 2 cut(s) 91, 258
DpnI GATC 6 cut(s) 63, 250, 543, 558, 801, 907
DpnII GATC 6 cut(s) 61, 248, 541, 556, 799, 905
Eam1104I CTCTTC 2 cut(s) 204, 207
EarI CTCTTC 2 cut(s) 204, 207
Eco130I CCWWGG 2 cut(s) 613, 747
Eco47I GGWCC 1 cut(s) 610
Eco57I CTGAAG 1 cut(s) 987
EcoRII CCWGG 1 cut(s) 779
EcoT14I CCWWGG 2 cut(s) 613, 747
ErhI CCWWGG 2 cut(s) 613, 747
Esp3I CGTCTC 1 cut(s) 983
FaeI CATG 5 cut(s) 200, 509, 680, 751, 806
FaqI GGGAC 3 cut(s) 54, 485, 1024
FatI CATG 5 cut(s) 196, 505, 676, 747, 802
FauI CCCGC 1 cut(s) 115
Fnu4HI GCNGC 2 cut(s) 819, 822
FokI GGATG 1 cut(s) 38
Fsp4HI GCNGC 2 cut(s) 819, 822
FspBI CTAG 4 cut(s) 396, 443, 539, 644
GlaI GCGC 2 cut(s) 255, 640
GluI GCNGC 2 cut(s) 819, 822
GsaI CCCAGC 1 cut(s) 40
GsuI CTGGAG 1 cut(s) 29
HaeIII GGCC 1 cut(s) 373
HapII CCGG 2 cut(s) 23, 245
HhaI GCGC 2 cut(s) 256, 641
Hin1II CATG 5 cut(s) 200, 509, 680, 751, 806
Hin6I GCGC 2 cut(s) 254, 639
HinP1I GCGC 2 cut(s) 254, 639
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 4 cut(s) 42, 89, 886, 1037
HpaI GTTAAC 1 cut(s) 97
HpaII CCGG 2 cut(s) 23, 245
HphI GGTGA 4 cut(s) 71, 536, 551, 800
Hpy166II GTNNAC 2 cut(s) 97, 946
Hpy188I TCNGA 1 cut(s) 967
Hpy188III TCNNGA 4 cut(s) 206, 333, 572, 668
Hpy8I GTNNAC 2 cut(s) 97, 946
HpyAV CCTTC 4 cut(s) 50, 384, 728, 962
HpyCH4III ACNGT 1 cut(s) 629
HpyCH4IV ACGT 1 cut(s) 1021
HpyCH4V TGCA 4 cut(s) 277, 583, 824, 946
HpyF10VI GCNNNNNNNGC 1 cut(s) 268
HpyF3I CTNAG 2 cut(s) 91, 258
HpySE526I ACGT 1 cut(s) 1021
Hsp92II CATG 5 cut(s) 200, 509, 680, 751, 806
HspAI GCGC 2 cut(s) 254, 639
KspAI GTTAAC 1 cut(s) 97
Kzo9I GATC 6 cut(s) 61, 248, 541, 556, 799, 905
LguI GCTCTTC 2 cut(s) 204, 207
LmnI GCTCC 1 cut(s) 783
Lsp1109I GCAGC 2 cut(s) 805, 808
LweI GCATC 2 cut(s) 145, 290
MaeI CTAG 4 cut(s) 396, 443, 539, 644
MaeII ACGT 1 cut(s) 1021
MaeIII GTNAC 5 cut(s) 86, 216, 303, 805, 1017
MalI GATC 6 cut(s) 63, 250, 543, 558, 801, 907
MbiI CCGCTC 1 cut(s) 786
MboI GATC 6 cut(s) 61, 248, 541, 556, 799, 905
MflI RGATCY 1 cut(s) 248
MhlI GDGCHC 1 cut(s) 948
MlyI GAGTC 3 cut(s) 36, 83, 1046
MnlI CCTC 6 cut(s) 226, 229, 349, 513, 544, 934
MseI TTAA 4 cut(s) 96, 135, 519, 840
MspI CCGG 2 cut(s) 23, 245
MspR9I CCNGG 3 cut(s) 23, 246, 781
MvaI CCWGG 1 cut(s) 781
MvnI CGCG 1 cut(s) 639
MwoI GCNNNNNNNGC 1 cut(s) 268
NciI CCSGG 2 cut(s) 23, 246
NcoI CCATGG 1 cut(s) 747
NdeII GATC 6 cut(s) 61, 248, 541, 556, 799, 905
NlaIII CATG 5 cut(s) 200, 509, 680, 751, 806
NlaIV GGNNCC 1 cut(s) 495
NmuCI GTSAC 4 cut(s) 86, 216, 303, 1017
NspV TTCGAA 1 cut(s) 71
PaqCI CACCTGC 1 cut(s) 881
PciSI GCTCTTC 2 cut(s) 204, 207
PfeI GAWTC 1 cut(s) 886
PfoI TCCNGGA 1 cut(s) 779
PkrI GCNGC 2 cut(s) 820, 823
PleI GAGTC 3 cut(s) 36, 83, 1045
PpsI GAGTC 3 cut(s) 36, 83, 1045
Ppu21I YACGTR 1 cut(s) 1022
PshBI ATTAAT 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 779
PspFI CCCAGC 1 cut(s) 36
PspGI CCWGG 1 cut(s) 779
PspN4I GGNNCC 1 cut(s) 495
PspPI GGNCC 2 cut(s) 371, 610
PstI CTGCAG 1 cut(s) 826
PsuI RGATCY 1 cut(s) 248
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
SapI GCTCTTC 2 cut(s) 204, 207
SaqAI TTAA 4 cut(s) 96, 135, 519, 840
SatI GCNGC 2 cut(s) 819, 822
Sau3AI GATC 6 cut(s) 61, 248, 541, 556, 799, 905
Sau96I GGNCC 2 cut(s) 371, 610
SchI GAGTC 3 cut(s) 36, 83, 1046
ScrFI CCNGG 3 cut(s) 23, 246, 781
SduI GDGCHC 1 cut(s) 948
SfaNI GCATC 2 cut(s) 145, 290
SfcI CTRYAG 1 cut(s) 822
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 1 cut(s) 610
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
SsiI CCGC 4 cut(s) 122, 158, 637, 786
SspMI CTAG 4 cut(s) 396, 443, 539, 644
StyD4I CCNGG 3 cut(s) 21, 244, 779
StyI CCWWGG 2 cut(s) 613, 747
TaaI ACNGT 1 cut(s) 629
TaiI ACGT 1 cut(s) 1024
TaqI TCGA 2 cut(s) 71, 908
TfiI GAWTC 1 cut(s) 886
Tru1I TTAA 4 cut(s) 96, 135, 519, 840
Tru9I TTAA 4 cut(s) 96, 135, 519, 840
TseFI GTSAC 4 cut(s) 86, 216, 303, 1017
TseI GCWGC 2 cut(s) 818, 821
Tsp45I GTSAC 4 cut(s) 86, 216, 303, 1017
TspDTI ATGAA 6 cut(s) 198, 378, 420, 471, 693, 747
TspGWI ACGGA 1 cut(s) 54
VneI GTGCAC 1 cut(s) 944
VpaK11BI GGWCC 1 cut(s) 610
VspI ATTAAT 1 cut(s) 519
XapI RAATTY 2 cut(s) 680, 843
XspI CTAG 4 cut(s) 396, 443, 539, 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.