FvH4_6g16161

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001571
Physical Location & Seq
Forward (+)
10190455 .. 10196146
5692 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16161.t3

Sequence Viewer

Length: 1005 bp
ATGGCTGGCCAAGAGCATGAGTCAGGTATTATGCAGTGGGCTCTTAGTCTTCTTGATGGCAATCCAGCTTATGATTCTGTCCAATATGGTGACATGATACAACATGATGCTAATGACATCTATCGCGGACAATATATTAGAGGTCAATATGGTGACACGATACAACATGATGCTAATGACATCTATCCTGGACAGTATGTTAGAGCTCAATATGGTACTGAACCTAACCATGTAGAGAATGATGAGATGATTGCACACACACTTCAAGAAGAATATTCACGACTATCTGTAGCAGAAGTCTCTGGATACTCACATGGAGATGAAGAGCAATCACAAGCATCTACTCATGCAGATAATTGGCACGGTCTGTCCAGAAGAAACTATTGTTCAGATGGTGATTATCCAAATGAAGGAGCATGCACAATGGTGCCTTCTAGTTCATGTTCTAGCCCGGGTCAGCTGGAGCTAAGTGATGAGTGTGCAGTTGATGGTGAAGTAGGCTGGAACCTCAATGAAATGATGCCTGTTCCTCATATTCCTAGAATTAATGGAGAAATTCCTTCAATTGATGAAGCAACATCAGATCATCAAAGGCTGCTTGACAGACTGGAATTATATGGTGTCGTTGAGCATAAAGTTCAAGGAGATGGTAACTGTCAGTTTCGTGCTTTATCAGACCAACTGTATCACTCACCAGACAACCACGATGATGTTAGAAGGAGAGTCGTAAATCAGCTTAAGTCTCACCCAGAAATTTACGAGGGATATGTTCCTATGGCATACGATGACTATCTGGAGAAGATGTCAAGGAGTGGCGAGTGGGGTGACCATGTTACATTGCAGGCTGCTGCTGATTCATATGGTGTGAAAATTCTTGTGTTGACTTCATTTAAGGATACATGTTGCATAGAGATTCTTCCTAATGTCCAACCGTCAAAACGAGTTATTATATTGAGTTTCTGGGCAGAGGTGCATTACAATTCAATTTATCCTCAAGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

335

Amino Acids

37.67

Weight (kDa)

4.58

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 214 - 300 3.9e-12 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 427
AccII CGCG 1 cut(s) 126
AciI CCGC 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 7
AcsI RAATTY 3 cut(s) 555, 753, 870
AfaI GTAC 1 cut(s) 217
AfiI CCNNNNNNNGG 1 cut(s) 410
AflII CTTAAG 1 cut(s) 737
AflIII ACRYGT 1 cut(s) 899
AgsI TTSAA 4 cut(s) 266, 564, 641, 984
AjnI CCWGG 1 cut(s) 187
AleI CACNNNNGTG 1 cut(s) 425
AluBI AGCT 5 cut(s) 68, 206, 460, 466, 736
AluI AGCT 5 cut(s) 68, 206, 460, 466, 736
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 2 cut(s) 304, 747
Ama87I CYCGRG 1 cut(s) 451
AoxI GGCC 1 cut(s) 7
ApeKI GCWGC 3 cut(s) 595, 845, 848
ApoI RAATTY 3 cut(s) 555, 753, 870
AseI ATTAAT 1 cut(s) 546
AsuC2I CCSGG 2 cut(s) 452, 453
AsuHPI GGTGA 7 cut(s) 101, 164, 407, 503, 684, 737, 836
AvaI CYCGRG 1 cut(s) 451
BalI TGGCCA 1 cut(s) 9
BanI GGYRCC 1 cut(s) 427
BanII GRGCYC 2 cut(s) 43, 208
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 3 cut(s) 582, 832, 835
BccI CCATC 4 cut(s) 50, 386, 482, 641
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 2 cut(s) 299, 889
BcnI CCSGG 2 cut(s) 452, 453
BcoDI GTCTC 2 cut(s) 304, 747
BfaI CTAG 3 cut(s) 435, 447, 540
BfmI CTRYAG 1 cut(s) 288
BfrI CTTAAG 1 cut(s) 737
BfuI GTATCC 2 cut(s) 299, 889
BisI GCNGC 3 cut(s) 596, 846, 849
BlsI GCNGC 3 cut(s) 597, 847, 850
Bme1390I CCNGG 3 cut(s) 189, 452, 453
BmeT110I CYCGRG 1 cut(s) 451
BmiI GGNNCC 2 cut(s) 429, 506
BmrFI CCNGG 3 cut(s) 189, 452, 453
BmsI GCATC 4 cut(s) 97, 160, 347, 510
BpiI GAAGAC 1 cut(s) 41
BpmI CTGGAG 2 cut(s) 482, 815
BpuEI CTTGAG 1 cut(s) 978
BpuMI CCSGG 2 cut(s) 452, 453
BsaBI GATNNNNATC 2 cut(s) 60, 789
BsaJI CCNNGG 1 cut(s) 451
BsaXI ACNNNNNCTCC 2 cut(s) 788, 818
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 612
Bse3DI GCAATG 1 cut(s) 836
Bse8I GATNNNNATC 2 cut(s) 60, 789
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 451
BseJI GATNNNNATC 2 cut(s) 60, 789
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMI GCAATG 1 cut(s) 836
BseNI ACTGG 1 cut(s) 612
BseXI GCAGC 3 cut(s) 582, 832, 835
BsgI GTGCAG 1 cut(s) 501
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 9
BshNI GGYRCC 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 451
BsiSI CCGG 1 cut(s) 452
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 2 cut(s) 304, 747
BsnI GGCC 1 cut(s) 9
BsoBI CYCGRG 1 cut(s) 451
Bsp1286I GDGCHC 2 cut(s) 43, 208
Bsp143I GATC 1 cut(s) 583
BspACI CCGC 1 cut(s) 126
BspANI GGCC 1 cut(s) 9
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 2 cut(s) 429, 506
BspQI GCTCTTC 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 427
BspTI CTTAAG 1 cut(s) 737
BsrDI GCAATG 1 cut(s) 836
BsrI ACTGG 1 cut(s) 612
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 1 cut(s) 583
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 5 cut(s) 195, 365, 656, 684, 933
Bst6I CTCTTC 1 cut(s) 318
BstAFI CTTAAG 1 cut(s) 737
BstC8I GCNNGC 3 cut(s) 7, 418, 843
BstDEI CTNAG 2 cut(s) 44, 467
BstEII GGTNACC 1 cut(s) 824
BstFNI CGCG 1 cut(s) 126
BstKTI GATC 1 cut(s) 586
BstMAI GTCTC 2 cut(s) 304, 747
BstMBI GATC 1 cut(s) 583
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 2 cut(s) 420, 903
BstPI GGTNACC 1 cut(s) 824
BstSCI CCNGG 3 cut(s) 187, 450, 451
BstSFI CTRYAG 1 cut(s) 288
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 3 cut(s) 582, 832, 835
BstV2I GAAGAC 1 cut(s) 41
BsuI GTATCC 2 cut(s) 299, 889
BsuRI GGCC 1 cut(s) 9
BtsI GCAGTG 1 cut(s) 41
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 3 cut(s) 7, 418, 843
Cfr9I CCCGGG 1 cut(s) 451
Csp6I GTAC 1 cut(s) 216
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 2 cut(s) 44, 467
DpnI GATC 1 cut(s) 585
DpnII GATC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 7
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 206
Eco24I GRGCYC 2 cut(s) 43, 208
Eco53kI GAGCTC 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 451
Eco91I GGTNACC 1 cut(s) 824
EcoICRI GAGCTC 1 cut(s) 206
EcoO65I GGTNACC 1 cut(s) 824
EcoRII CCWGG 1 cut(s) 187
EcoT38I GRGCYC 2 cut(s) 43, 208
FauNDI CATATG 1 cut(s) 859
Fnu4HI GCNGC 3 cut(s) 596, 846, 849
FriOI GRGCYC 2 cut(s) 43, 208
Fsp4HI GCNGC 3 cut(s) 596, 846, 849
FspBI CTAG 3 cut(s) 435, 447, 540
GluI GCNGC 3 cut(s) 596, 846, 849
GsuI CTGGAG 2 cut(s) 482, 815
HaeIII GGCC 1 cut(s) 9
HapII CCGG 1 cut(s) 452
HincII GTYRAC 1 cut(s) 882
HindII GTYRAC 1 cut(s) 882
HinfI GANTC 5 cut(s) 20, 74, 723, 854, 913
HpaII CCGG 1 cut(s) 452
HphI GGTGA 7 cut(s) 101, 164, 407, 503, 684, 737, 836
Hpy166II GTNNAC 1 cut(s) 882
Hpy188I TCNGA 3 cut(s) 391, 583, 676
Hpy188III TCNNGA 7 cut(s) 53, 266, 279, 303, 372, 794, 995
Hpy8I GTNNAC 1 cut(s) 882
HpyAV CCTTC 4 cut(s) 404, 441, 570, 711
HpyCH4III ACNGT 5 cut(s) 195, 365, 656, 684, 933
HpyCH4V TGCA 8 cut(s) 34, 254, 350, 420, 482, 841, 906, 973
HpyF3I CTNAG 2 cut(s) 44, 467
Kzo9I GATC 1 cut(s) 583
LguI GCTCTTC 1 cut(s) 318
LmnI GCTCC 2 cut(s) 413, 463
Lsp1109I GCAGC 3 cut(s) 582, 832, 835
LweI GCATC 4 cut(s) 97, 160, 347, 510
MaeI CTAG 3 cut(s) 435, 447, 540
MaeIII GTNAC 5 cut(s) 89, 152, 650, 824, 832
MalI GATC 1 cut(s) 585
MboI GATC 1 cut(s) 583
MboII GAAGA 6 cut(s) 41, 281, 335, 387, 811, 908
MfeI CAATTG 1 cut(s) 564
MhlI GDGCHC 2 cut(s) 43, 208
MlsI TGGCCA 1 cut(s) 9
MluCI AATT 9 cut(s) 355, 543, 555, 564, 611, 753, 870, 979, 984
MluNI TGGCCA 1 cut(s) 9
MlyI GAGTC 2 cut(s) 29, 732
MmeI TCCRAC 1 cut(s) 952
MnlI CCTC 6 cut(s) 134, 518, 540, 754, 961, 1002
Mox20I TGGCCA 1 cut(s) 9
MscI TGGCCA 1 cut(s) 9
MseI TTAA 3 cut(s) 546, 738, 891
MslI CAYNNNNRTG 3 cut(s) 318, 425, 708
Msp20I TGGCCA 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 460
MspCI CTTAAG 1 cut(s) 737
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 3 cut(s) 189, 452, 453
MunI CAATTG 1 cut(s) 564
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 126
NciI CCSGG 2 cut(s) 452, 453
NdeI CATATG 1 cut(s) 859
NdeII GATC 1 cut(s) 583
NlaIV GGNNCC 2 cut(s) 429, 506
NmuCI GTSAC 3 cut(s) 89, 152, 824
NspI RCATGY 2 cut(s) 420, 903
OliI CACNNNNGTG 1 cut(s) 425
PaeI GCATGC 1 cut(s) 420
PciI ACATGT 1 cut(s) 899
PciSI GCTCTTC 1 cut(s) 318
PfeI GAWTC 3 cut(s) 74, 854, 913
PfoI TCCNGGA 1 cut(s) 187
PkrI GCNGC 3 cut(s) 597, 847, 850
PleI GAGTC 2 cut(s) 28, 731
PpsI GAGTC 2 cut(s) 28, 731
PscI ACATGT 1 cut(s) 899
PshBI ATTAAT 1 cut(s) 546
Psp124BI GAGCTC 1 cut(s) 208
Psp6I CCWGG 1 cut(s) 187
PspEI GGTNACC 1 cut(s) 824
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 429, 506
PvuII CAGCTG 1 cut(s) 460
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
RseI CAYNNNNRTG 3 cut(s) 318, 425, 708
SacI GAGCTC 1 cut(s) 208
SapI GCTCTTC 1 cut(s) 318
SaqAI TTAA 3 cut(s) 546, 738, 891
SatI GCNGC 3 cut(s) 596, 846, 849
Sau3AI GATC 1 cut(s) 583
SchI GAGTC 2 cut(s) 29, 732
ScrFI CCNGG 3 cut(s) 189, 452, 453
SduI GDGCHC 2 cut(s) 43, 208
SfaNI GCATC 4 cut(s) 97, 160, 347, 510
SfcI CTRYAG 1 cut(s) 288
SmaI CCCGGG 1 cut(s) 453
SmiMI CAYNNNNRTG 3 cut(s) 318, 425, 708
SmlI CTYRAG 2 cut(s) 737, 993
SmoI CTYRAG 2 cut(s) 737, 993
SphI GCATGC 1 cut(s) 420
Sse9I AATT 9 cut(s) 355, 543, 555, 564, 611, 753, 870, 979, 984
SsiI CCGC 1 cut(s) 126
SspI AATATT 1 cut(s) 275
SspMI CTAG 3 cut(s) 435, 447, 540
SstI GAGCTC 1 cut(s) 208
StyD4I CCNGG 3 cut(s) 187, 450, 451
TaaI ACNGT 5 cut(s) 195, 365, 656, 684, 933
TasI AATT 9 cut(s) 355, 543, 555, 564, 611, 753, 870, 979, 984
TfiI GAWTC 3 cut(s) 74, 854, 913
Tru1I TTAA 3 cut(s) 546, 738, 891
Tru9I TTAA 3 cut(s) 546, 738, 891
TscAI CASTG 1 cut(s) 41
TseFI GTSAC 3 cut(s) 89, 152, 824
TseI GCWGC 3 cut(s) 595, 845, 848
Tsp45I GTSAC 3 cut(s) 89, 152, 824
TspDTI ATGAA 7 cut(s) 336, 423, 429, 528, 585, 846, 876
TspMI CCCGGG 1 cut(s) 451
TspRI CASTG 1 cut(s) 41
Vha464I CTTAAG 1 cut(s) 737
VspI ATTAAT 1 cut(s) 546
XapI RAATTY 3 cut(s) 555, 753, 870
XceI RCATGY 2 cut(s) 420, 903
XmaI CCCGGG 1 cut(s) 451
XspI CTAG 3 cut(s) 435, 447, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.