Rw3G014620

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001571
Physical Location & Seq
Forward (+)
14855083 .. 14861849
6767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G014620.1

Sequence Viewer

Length: 1002 bp
ATGGCTGGCCAAGAGCATGAGTCTGGTATTATGCAGTGGGCTCTTAGTCTACTTGATGGCGATCCAGCTTATAATACTGTCTATAATGGTGACATGATACAACACGATGCTAATGACATCTACCACAGACAATATTTTAGAGGTCAATATGGTACTGAATCTAACCATGTAGAGAATGATGAGATCATTGCACACACACTTCAAGAAGAATATTCACGACTATCTGTAGCAGAAGCCTCTCGATACTCACATGGAGATGAAGAGCAATCACAAGCATCTACTCATGCAGATGATTGGTATAGTTTGTCCAGAAGGAACTATTGTTCAGACAGTGATTATCCAAATGAAGGAGCATGCACAGTGGTGCCTTCTAGTTCATGTTCTAGCCCAGGTCGTTTGGAGTTAACTGATGAGTGTGCAGTTGACGGTGAAGTAGGCTGGAGCTTCAATGAAATGATGCCCGTTCCTCATATTCCTAGAATTAATGGAGAAATTCCTTCAATTGATGAAGCTACTTCAGATCATCAGAGGCTGCTTGACAGACTGCAATTATATGGCGTCGTGGAGCGTAAGGTTCAAGGAGATGGTAACTGTCAGTTTAGTGCTTTATCAGACCAATTGTATCACTCACCAGACCACCACAATGATGTGAGAAGGAAAGTTGTAAATCAGCTTAAGTCTCACCCAGAAATTTACGAGGGATATGTTCCTATGGCATACGATGACTATTTGGAGATGATGTCAAAGAGTGGCCAGTGGGGTGATCATGTTACATTGCAGGCTGCTGCTGATTCGCATGGTGTGAAAATATTTGTCTTCACTTCTTTTAAGGATACATGTTGCATAGAGATTCTTCCTAATGTCCAAACGTCAAAACGAGTTATTATGTTGAGTTTCTGGGCAGAGGTGCATTACAACTCAATTTATCCTCAAGAAGGTACACCCTCCTCAGATGAGTATAGAAAGAAGAAAAAGTGGTGGATATTTGGGAATAAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

333

Amino Acids

37.98

Weight (kDa)

4.93

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 193 - 279 4.4e-11 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 364
AccI GTMKAC 1 cut(s) 49
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 2 cut(s) 7, 751
AcsI RAATTY 2 cut(s) 492, 690
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 558
AfaI GTAC 2 cut(s) 154, 940
AfiI CCNNNNNNNGG 2 cut(s) 347, 935
AflII CTTAAG 1 cut(s) 674
AflIII ACRYGT 1 cut(s) 836
AgsI TTSAA 4 cut(s) 203, 448, 501, 578
AjnI CCWGG 1 cut(s) 388
AleI CACNNNNGTG 1 cut(s) 362
AloI GAACNNNNNNTCC 2 cut(s) 307, 339
AluBI AGCT 4 cut(s) 68, 444, 512, 673
AluI AGCT 4 cut(s) 68, 444, 512, 673
Alw26I GTCTC 1 cut(s) 684
AlwI GGATC 1 cut(s) 56
AlwNI CAGNNNCTG 1 cut(s) 532
AoxI GGCC 2 cut(s) 7, 751
ApeKI GCWGC 3 cut(s) 532, 782, 785
ApoI RAATTY 2 cut(s) 492, 690
AseI ATTAAT 1 cut(s) 483
AsuHPI GGTGA 5 cut(s) 101, 440, 621, 674, 773
BalI TGGCCA 2 cut(s) 9, 753
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 808
BbvI GCAGC 3 cut(s) 519, 769, 772
BccI CCATC 2 cut(s) 50, 578
BciT130I CCWGG 1 cut(s) 390
BciVI GTATCC 1 cut(s) 826
BclI TGATCA 1 cut(s) 763
BcoDI GTCTC 1 cut(s) 684
BfaI CTAG 3 cut(s) 372, 384, 477
BfmI CTRYAG 1 cut(s) 225
BfrI CTTAAG 1 cut(s) 674
BfuI GTATCC 1 cut(s) 826
BisI GCNGC 3 cut(s) 533, 783, 786
BlsI GCNGC 3 cut(s) 534, 784, 787
Bme1390I CCNGG 1 cut(s) 390
BmiI GGNNCC 1 cut(s) 366
BmrFI CCNGG 1 cut(s) 390
BmsI GCATC 3 cut(s) 97, 284, 447
BpiI GAAGAC 1 cut(s) 808
BpmI CTGGAG 1 cut(s) 460
BpuEI CTTGAG 1 cut(s) 915
BsaBI GATNNNNATC 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 558
BsaJI CCNNGG 1 cut(s) 388
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 935
Bse1I ACTGG 1 cut(s) 754
Bse3DI GCAATG 2 cut(s) 186, 773
Bse8I GATNNNNATC 1 cut(s) 60
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 1 cut(s) 388
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 2 cut(s) 347, 935
BseMI GCAATG 2 cut(s) 186, 773
BseMII CTCAG 1 cut(s) 963
BseNI ACTGG 1 cut(s) 754
BseRI GAGGAG 1 cut(s) 937
BseXI GCAGC 3 cut(s) 519, 769, 772
BsgI GTGCAG 1 cut(s) 438
BshFI GGCC 2 cut(s) 9, 753
BshNI GGYRCC 1 cut(s) 364
BslI CCNNNNNNNGG 2 cut(s) 347, 935
BsmAI GTCTC 1 cut(s) 684
BsnI GGCC 2 cut(s) 9, 753
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 4 cut(s) 61, 183, 520, 763
BspANI GGCC 2 cut(s) 9, 753
BspCNI CTCAG 1 cut(s) 962
BspLI GGNNCC 1 cut(s) 366
BspPI GGATC 1 cut(s) 56
BspQI GCTCTTC 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 364
BspTI CTTAAG 1 cut(s) 674
BsrDI GCAATG 2 cut(s) 186, 773
BsrI ACTGG 1 cut(s) 754
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 4 cut(s) 61, 183, 520, 763
BssNI GRCGYC 1 cut(s) 558
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 5 cut(s) 79, 332, 361, 428, 593
Bst6I CTCTTC 1 cut(s) 255
BstACI GRCGYC 1 cut(s) 558
BstAFI CTTAAG 1 cut(s) 674
BstC8I GCNNGC 3 cut(s) 7, 355, 780
BstDEI CTNAG 2 cut(s) 44, 949
BstENI CCTNNNNNAGG 1 cut(s) 933
BstKTI GATC 4 cut(s) 64, 186, 523, 766
BstMAI GTCTC 1 cut(s) 684
BstMBI GATC 4 cut(s) 61, 183, 520, 763
BstNI CCWGG 1 cut(s) 390
BstNSI RCATGY 2 cut(s) 357, 840
BstSCI CCNGG 1 cut(s) 388
BstSFI CTRYAG 1 cut(s) 225
BstV1I GCAGC 3 cut(s) 519, 769, 772
BstV2I GAAGAC 1 cut(s) 808
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 2 cut(s) 9, 753
BtsI GCAGTG 1 cut(s) 41
BtsIMutI CAGTG 4 cut(s) 41, 337, 366, 761
Cac8I GCNNGC 3 cut(s) 7, 355, 780
CaiI CAGNNNCTG 1 cut(s) 532
CseI GACGC 1 cut(s) 547
Csp6I GTAC 2 cut(s) 153, 939
CviQI GTAC 2 cut(s) 153, 939
DdeI CTNAG 2 cut(s) 44, 949
DpnI GATC 4 cut(s) 63, 185, 522, 765
DpnII GATC 4 cut(s) 61, 183, 520, 763
EaeI YGGCCR 2 cut(s) 7, 751
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 501
EcoNI CCTNNNNNAGG 1 cut(s) 933
EcoRII CCWGG 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 43
FbaI TGATCA 1 cut(s) 763
FblI GTMKAC 1 cut(s) 49
Fnu4HI GCNGC 3 cut(s) 533, 783, 786
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 3 cut(s) 533, 783, 786
FspBI CTAG 3 cut(s) 372, 384, 477
GluI GCNGC 3 cut(s) 533, 783, 786
GsuI CTGGAG 1 cut(s) 460
HaeIII GGCC 2 cut(s) 9, 753
HgaI GACGC 1 cut(s) 547
Hin1I GRCGYC 1 cut(s) 558
HincII GTYRAC 2 cut(s) 405, 424
HindII GTYRAC 2 cut(s) 405, 424
HinfI GANTC 4 cut(s) 20, 158, 791, 850
HpaI GTTAAC 1 cut(s) 405
HphI GGTGA 5 cut(s) 101, 440, 621, 674, 773
Hpy166II GTNNAC 4 cut(s) 50, 405, 424, 941
Hpy188I TCNGA 5 cut(s) 328, 520, 528, 613, 952
Hpy188III TCNNGA 5 cut(s) 203, 216, 240, 309, 932
Hpy8I GTNNAC 4 cut(s) 50, 405, 424, 941
Hpy99I CGWCG 1 cut(s) 563
HpyAV CCTTC 6 cut(s) 306, 341, 378, 507, 648, 929
HpyCH4III ACNGT 5 cut(s) 79, 332, 361, 428, 593
HpyCH4IV ACGT 1 cut(s) 869
HpyCH4V TGCA 9 cut(s) 34, 191, 287, 357, 419, 547, 778, 843, 910
HpyF3I CTNAG 2 cut(s) 44, 949
HpySE526I ACGT 1 cut(s) 869
Hsp92I GRCGYC 1 cut(s) 558
Ksp22I TGATCA 1 cut(s) 763
KspAI GTTAAC 1 cut(s) 405
Kzo9I GATC 4 cut(s) 61, 183, 520, 763
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 3 cut(s) 350, 441, 565
Lsp1109I GCAGC 3 cut(s) 519, 769, 772
LweI GCATC 3 cut(s) 97, 284, 447
MaeI CTAG 3 cut(s) 372, 384, 477
MaeII ACGT 1 cut(s) 869
MaeIII GTNAC 3 cut(s) 89, 587, 769
MalI GATC 4 cut(s) 63, 185, 522, 765
MboI GATC 4 cut(s) 61, 183, 520, 763
MboII GAAGA 5 cut(s) 218, 272, 808, 845, 979
MfeI CAATTG 2 cut(s) 501, 617
MhlI GDGCHC 1 cut(s) 43
MlsI TGGCCA 2 cut(s) 9, 753
MluCI AATT 8 cut(s) 480, 492, 501, 548, 617, 690, 921, 997
MluNI TGGCCA 2 cut(s) 9, 753
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 9 cut(s) 134, 247, 477, 522, 691, 898, 939, 955, 958
Mox20I TGGCCA 2 cut(s) 9, 753
MscI TGGCCA 2 cut(s) 9, 753
MseI TTAA 4 cut(s) 404, 483, 675, 828
MslI CAYNNNNRTG 5 cut(s) 255, 288, 362, 642, 645
Msp20I TGGCCA 2 cut(s) 9, 753
MspCI CTTAAG 1 cut(s) 674
MspR9I CCNGG 1 cut(s) 390
MunI CAATTG 2 cut(s) 501, 617
MvaI CCWGG 1 cut(s) 390
NdeII GATC 4 cut(s) 61, 183, 520, 763
NlaIV GGNNCC 1 cut(s) 366
NmuCI GTSAC 1 cut(s) 89
NspI RCATGY 2 cut(s) 357, 840
OliI CACNNNNGTG 1 cut(s) 362
PaeI GCATGC 1 cut(s) 357
PciI ACATGT 1 cut(s) 836
PciSI GCTCTTC 1 cut(s) 255
PfeI GAWTC 3 cut(s) 158, 791, 850
PkrI GCNGC 3 cut(s) 534, 784, 787
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PscI ACATGT 1 cut(s) 836
PshBI ATTAAT 1 cut(s) 483
PsiI TTATAA 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 532
RsaI GTAC 2 cut(s) 154, 940
RsaNI GTAC 2 cut(s) 153, 939
RseI CAYNNNNRTG 5 cut(s) 255, 288, 362, 642, 645
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 4 cut(s) 404, 483, 675, 828
SatI GCNGC 3 cut(s) 533, 783, 786
Sau3AI GATC 4 cut(s) 61, 183, 520, 763
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 390
SduI GDGCHC 1 cut(s) 43
SfaNI GCATC 3 cut(s) 97, 284, 447
SfcI CTRYAG 1 cut(s) 225
SmiMI CAYNNNNRTG 5 cut(s) 255, 288, 362, 642, 645
SmlI CTYRAG 2 cut(s) 674, 930
SmoI CTYRAG 2 cut(s) 674, 930
SphI GCATGC 1 cut(s) 357
Sse9I AATT 8 cut(s) 480, 492, 501, 548, 617, 690, 921, 997
SspI AATATT 3 cut(s) 134, 212, 810
SspMI CTAG 3 cut(s) 372, 384, 477
StyD4I CCNGG 1 cut(s) 388
TaaI ACNGT 5 cut(s) 79, 332, 361, 428, 593
TaiI ACGT 1 cut(s) 872
TaqI TCGA 1 cut(s) 241
TasI AATT 8 cut(s) 480, 492, 501, 548, 617, 690, 921, 997
TfiI GAWTC 3 cut(s) 158, 791, 850
Tru1I TTAA 4 cut(s) 404, 483, 675, 828
Tru9I TTAA 4 cut(s) 404, 483, 675, 828
TscAI CASTG 4 cut(s) 41, 337, 366, 761
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 3 cut(s) 532, 782, 785
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 5 cut(s) 273, 360, 366, 465, 522
TspRI CASTG 4 cut(s) 41, 337, 366, 761
Vha464I CTTAAG 1 cut(s) 674
VspI ATTAAT 1 cut(s) 483
XagI CCTNNNNNAGG 1 cut(s) 933
XapI RAATTY 2 cut(s) 492, 690
XceI RCATGY 2 cut(s) 357, 840
XmiI GTMKAC 1 cut(s) 49
XspI CTAG 3 cut(s) 372, 384, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.