pycom12g11450

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001571
Physical Location & Seq
Reverse (-)
13705082 .. 13707345
2264 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11450.1

Sequence Viewer

Length: 744 bp
ATGCAGGAGAAGCGCAATCACAAGCTTCATTTCTTGCAAATGAGTGGCATGGTCAATCTACAAGAGATTATTACTCAGACCATGGTTATGGCCAGGAAGAAGCTGATACTGTGGCGCCTTCCAGCTCATGTTCTAGCCCTGATCGATCATTTGGAGCTAACTTATGGATGTGCACTTGATGGTGAAGTAGGCTGGGGCTGGAATCCTGTTCCTCACATTCCTAGAATCAATGGAGAAATTCCTTCAATTGATGAAGCGACTTCAGATCATCAAAGGCTAGTTGACAGGCTTCAATTATATGGCTTCGTCGAACACAAGGTGGCAGGAGATGGCAACTGCCAGTTCCATGCTTTATCAGACCAGTTGTATCATACACCTGACAACCACGAGTATGTGAGAAAGCAAGTTGTAAATCAGCTTAGGTCTCATCCGGAGATATACGAGGGATATGTTCCCATGGCTTATGATGACTATTTGGAGAAGATGTCCAAGGATGGTGAGTGGGGTGACCATGTCTCATTACAGGCGGCTGCTGATTCGTACAAAGTGAAAATATTTGTCTTGACGTCGTTTAAGGATAACTGTTGCATAGAGATTCTTCCTAATATTCAAACTTCAAAACAAGTTATTTATTTGAGTTTCTGGGCGGAGGTGCATTACAACTCAATTTATCCTCAAGGAGGCACACCCTCATATGAGAACAGAAAAAAGAAAAAATGGCGGATATTTGGGAATAAGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

248

Amino Acids

28.69

Weight (kDa)

6.7

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 109 - 195 7.7e-15 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 569
AccB1I GGYRCC 1 cut(s) 114
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 3 cut(s) 527, 647, 721
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 1 cut(s) 237
AcuI CTGAAG 1 cut(s) 246
AcyI GRCGYC 2 cut(s) 115, 566
AdeI CACNNNGTG 1 cut(s) 319
AfaI GTAC 1 cut(s) 542
AfiI CCNNNNNNNGG 1 cut(s) 680
AgsI TTSAA 4 cut(s) 246, 293, 611, 618
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 5 cut(s) 25, 103, 125, 157, 418
AluI AGCT 5 cut(s) 25, 103, 125, 157, 418
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 2 cut(s) 429, 520
Alw44I GTGCAC 1 cut(s) 171
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 1 cut(s) 90
ApaLI GTGCAC 1 cut(s) 171
ApeKI GCWGC 1 cut(s) 530
ApoI RAATTY 1 cut(s) 237
AspLEI GCGC 2 cut(s) 15, 117
AsuHPI GGTGA 3 cut(s) 194, 509, 518
BaeGI GKGCMC 1 cut(s) 175
BalI TGGCCA 1 cut(s) 92
BanI GGYRCC 1 cut(s) 114
BauI CACGAG 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 1 cut(s) 517
BccI CCATC 3 cut(s) 173, 323, 488
BciT130I CCWGG 1 cut(s) 94
BcoDI GTCTC 2 cut(s) 429, 520
BfaI CTAG 3 cut(s) 134, 222, 278
BfoI RGCGCY 1 cut(s) 118
BisI GCNGC 2 cut(s) 528, 531
BlsI GCNGC 2 cut(s) 529, 532
Bme1390I CCNGG 1 cut(s) 94
BmiI GGNNCC 1 cut(s) 116
BmrFI CCNGG 1 cut(s) 94
Bpu10I CCTNAGC 1 cut(s) 419
BpuEI CTTGAG 1 cut(s) 660
Bsa29I ATCGAT 1 cut(s) 144
BsaHI GRCGYC 2 cut(s) 115, 566
BsaI GGTCTC 1 cut(s) 429
BsaJI CCNNGG 3 cut(s) 81, 456, 489
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 1 cut(s) 680
Bse1I ACTGG 2 cut(s) 340, 361
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 1 cut(s) 94
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 3 cut(s) 81, 456, 489
BseGI GGATG 3 cut(s) 173, 427, 499
BseLI CCNNNNNNNGG 1 cut(s) 680
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 2 cut(s) 340, 361
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 1 cut(s) 517
BseYI CCCAGC 1 cut(s) 192
BshFI GGCC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 114
BshVI ATCGAT 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 175
BsiSI CCGG 1 cut(s) 431
BslI CCNNNNNNNGG 1 cut(s) 680
BsmAI GTCTC 2 cut(s) 429, 520
BsnI GGCC 1 cut(s) 92
Bso31I GGTCTC 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 175
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 3 cut(s) 141, 145, 265
Bsp19I CCATGG 2 cut(s) 81, 456
BspACI CCGC 3 cut(s) 527, 647, 721
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 1 cut(s) 88
BspDI ATCGAT 1 cut(s) 144
BspEI TCCGGA 1 cut(s) 430
BspLI GGNNCC 1 cut(s) 116
BspT107I GGYRCC 1 cut(s) 114
BspTNI GGTCTC 1 cut(s) 429
BsrI ACTGG 2 cut(s) 340, 361
BssECI CCNNGG 3 cut(s) 81, 456, 489
BssMI GATC 3 cut(s) 141, 145, 265
BssNI GRCGYC 2 cut(s) 115, 566
BssSI CACGAG 1 cut(s) 386
BssT1I CCWWGG 3 cut(s) 81, 456, 489
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 94
Bst4CI ACNGT 2 cut(s) 111, 584
BstACI GRCGYC 2 cut(s) 115, 566
BstDEI CTNAG 2 cut(s) 75, 419
BstDSI CCRYGG 2 cut(s) 81, 456
BstEII GGTNACC 1 cut(s) 506
BstENI CCTNNNNNAGG 1 cut(s) 678
BstF5I GGATG 3 cut(s) 173, 427, 499
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 2 cut(s) 15, 117
BstKTI GATC 3 cut(s) 144, 148, 268
BstMAI GTCTC 2 cut(s) 429, 520
BstMBI GATC 3 cut(s) 141, 145, 265
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 94
BstPI GGTNACC 1 cut(s) 506
BstSCI CCNGG 1 cut(s) 92
BstSLI GKGCMC 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 517
BstXI CCANNNNNNTGG 1 cut(s) 88
Bsu15I ATCGAT 1 cut(s) 144
BsuRI GGCC 1 cut(s) 92
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 2 cut(s) 81, 456
BtsCI GGATG 3 cut(s) 173, 427, 499
CfoI GCGC 2 cut(s) 15, 117
ClaI ATCGAT 1 cut(s) 144
Csp6I GTAC 1 cut(s) 541
CviAII CATG 6 cut(s) 49, 82, 128, 347, 457, 512
CviQI GTAC 1 cut(s) 541
DdeI CTNAG 2 cut(s) 75, 419
DinI GGCGCC 1 cut(s) 116
DpnI GATC 3 cut(s) 143, 147, 267
DpnII GATC 3 cut(s) 141, 145, 265
DraIII CACNNNGTG 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 90
EciI GGCGGA 2 cut(s) 662, 736
Eco130I CCWWGG 3 cut(s) 81, 456, 489
Eco31I GGTCTC 1 cut(s) 429
Eco57I CTGAAG 1 cut(s) 246
Eco91I GGTNACC 1 cut(s) 506
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO65I GGTNACC 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 92
EcoT14I CCWWGG 3 cut(s) 81, 456, 489
EgeI GGCGCC 1 cut(s) 116
EheI GGCGCC 1 cut(s) 116
ErhI CCWWGG 3 cut(s) 81, 456, 489
FaeI CATG 6 cut(s) 52, 85, 131, 350, 460, 515
FatI CATG 6 cut(s) 48, 81, 127, 346, 456, 511
FauNDI CATATG 1 cut(s) 694
Fnu4HI GCNGC 2 cut(s) 528, 531
FokI GGATG 3 cut(s) 180, 414, 506
Fsp4HI GCNGC 2 cut(s) 528, 531
FspBI CTAG 3 cut(s) 134, 222, 278
GlaI GCGC 2 cut(s) 14, 116
GluI GCNGC 2 cut(s) 528, 531
GsaI CCCAGC 1 cut(s) 196
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 1 cut(s) 92
HapII CCGG 1 cut(s) 431
HhaI GCGC 2 cut(s) 15, 117
Hin1I GRCGYC 2 cut(s) 115, 566
Hin1II CATG 6 cut(s) 52, 85, 131, 350, 460, 515
Hin6I GCGC 2 cut(s) 13, 115
HinP1I GCGC 2 cut(s) 13, 115
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 4 cut(s) 202, 225, 536, 595
HpaII CCGG 1 cut(s) 431
HphI GGTGA 3 cut(s) 194, 509, 518
Hpy166II GTNNAC 2 cut(s) 173, 283
Hpy188I TCNGA 3 cut(s) 78, 265, 358
Hpy188III TCNNGA 2 cut(s) 431, 562
Hpy8I GTNNAC 2 cut(s) 173, 283
Hpy99I CGWCG 2 cut(s) 311, 571
HpyAV CCTTC 2 cut(s) 128, 252
HpyCH4III ACNGT 2 cut(s) 111, 584
HpyCH4IV ACGT 1 cut(s) 566
HpyCH4V TGCA 5 cut(s) 4, 37, 173, 588, 655
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 2 cut(s) 75, 419
HpySE526I ACGT 1 cut(s) 566
Hsp92I GRCGYC 2 cut(s) 115, 566
Hsp92II CATG 6 cut(s) 52, 85, 131, 350, 460, 515
HspAI GCGC 2 cut(s) 13, 115
KasI GGCGCC 1 cut(s) 114
Kpn2I TCCGGA 1 cut(s) 430
Kzo9I GATC 3 cut(s) 141, 145, 265
LmnI GCTCC 1 cut(s) 154
Lsp1109I GCAGC 1 cut(s) 517
MaeI CTAG 3 cut(s) 134, 222, 278
MaeII ACGT 1 cut(s) 566
MaeIII GTNAC 1 cut(s) 506
MalI GATC 3 cut(s) 143, 147, 267
MboI GATC 3 cut(s) 141, 145, 265
MboII GAAGA 3 cut(s) 109, 493, 590
MfeI CAATTG 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 175
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 4 cut(s) 237, 246, 293, 666
MluNI TGGCCA 1 cut(s) 92
Mly113I GGCGCC 1 cut(s) 115
MnlI CCTC 6 cut(s) 222, 436, 643, 674, 684, 700
Mox20I TGGCCA 1 cut(s) 92
MroI TCCGGA 1 cut(s) 430
MscI TGGCCA 1 cut(s) 92
MseI TTAA 1 cut(s) 573
MslI CAYNNNNRTG 2 cut(s) 86, 390
Msp20I TGGCCA 1 cut(s) 92
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 1 cut(s) 94
MunI CAATTG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 94
MwoI GCNNNNNNNGC 1 cut(s) 10
NarI GGCGCC 1 cut(s) 115
NcoI CCATGG 2 cut(s) 81, 456
NdeI CATATG 1 cut(s) 694
NdeII GATC 3 cut(s) 141, 145, 265
NlaIII CATG 6 cut(s) 52, 85, 131, 350, 460, 515
NlaIV GGNNCC 1 cut(s) 116
NmuCI GTSAC 1 cut(s) 506
PfeI GAWTC 4 cut(s) 202, 225, 536, 595
PflFI GACNNNGTC 1 cut(s) 512
PkrI GCNGC 2 cut(s) 529, 532
PluTI GGCGCC 1 cut(s) 118
Psp6I CCWGG 1 cut(s) 92
PspEI GGTNACC 1 cut(s) 506
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 1 cut(s) 116
PsyI GACNNNGTC 1 cut(s) 512
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
RseI CAYNNNNRTG 2 cut(s) 86, 390
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 2 cut(s) 528, 531
Sau3AI GATC 3 cut(s) 141, 145, 265
ScrFI CCNGG 1 cut(s) 94
SduI GDGCHC 1 cut(s) 175
SfoI GGCGCC 1 cut(s) 116
SmiMI CAYNNNNRTG 2 cut(s) 86, 390
SmlI CTYRAG 1 cut(s) 675
SmoI CTYRAG 1 cut(s) 675
Sse9I AATT 4 cut(s) 237, 246, 293, 666
SsiI CCGC 3 cut(s) 527, 647, 721
SspDI GGCGCC 1 cut(s) 114
SspI AATATT 2 cut(s) 555, 607
SspMI CTAG 3 cut(s) 134, 222, 278
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 3 cut(s) 81, 456, 489
TaaI ACNGT 2 cut(s) 111, 584
TaiI ACGT 1 cut(s) 569
TaqI TCGA 2 cut(s) 144, 309
TasI AATT 4 cut(s) 237, 246, 293, 666
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 4 cut(s) 202, 225, 536, 595
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TseFI GTSAC 1 cut(s) 506
TseI GCWGC 1 cut(s) 530
Tsp45I GTSAC 1 cut(s) 506
TspDTI ATGAA 2 cut(s) 17, 267
Tth111I GACNNNGTC 1 cut(s) 512
VneI GTGCAC 1 cut(s) 171
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 1 cut(s) 237
XspI CTAG 3 cut(s) 134, 222, 278
ZraI GACGTC 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.