RLG00000035200

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001571
Physical Location & Seq
Reverse (-)
62729473 .. 62733880
4408 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035200

Sequence Viewer

Length: 963 bp
ATGCTTACATACGAGCAAGACCCGGATGTTGTTCGCTGGGGTCTCCAGCTCTTTGAAGGTGATCCGTATTCGAATTGTGGGTATTGTGACTCAGTTAGCCAAGACGGCACTAGTTATTATCCGGGGCATTATTTTAAGGAAGATGTGGTGTATGATGCGAATTGTATAAATGTGGAATATGATGAACTTATTGCTCATGCTCTTCAAGAAGAGCTGTCACTACTTGATATAGAGGACGAGCCGGGATCTGCGCCTGAGCAAGTAGAGCATTTGCAAGCATCTGTCTTCCCACAAGATTGGTCACAGCAATCTATTGGAAATTATGGCTCTGGACAGGAGAGTGTTCAGGCAGAGGCAGATGAATTTGGGCCTTCTACTTCGTGTTCTAGCCCAGATGAAAAGTCATACTATGGAGATGAGTGGTCATATTCCCTAGAGCTGGCTAATGAATATACATTTGATGGAGAGAGGTTGAACCAGATGGTTCCTGTCCCTCATGTTCCTAAAATTAATGGTGATATACCCTCGCTAGATCAAGCAACTTTAGATCACCAAAGGCTTCTGGACAGGTTGCAGTTATATGGGTTGGTTGAAAATAAGGTCCAAGGAGATGGAAACTGTCAGTTCCGCGCTCTGGCTGACCAATTTTATCAATCTCCTGAACACCATGAATTTGTGAGACAACAAATTGTAAATCAGCTCAAGTCATACCCAGAGATATATGAAGGATATGTTCCCATGGCTTATGATGACTATTTGGAGAAGATGTCCAGGAGCGGTGAGTGGGGCGATCATGTTACTTTACAGGCTGCTGCAGATTTGTATGGAGTTAAAATTTTTGTCATAACATCTTTCAAGGACACCTGCTACATTGAGATTCTTCCTACTGCTGAAAGATCGAAACGAGGTGACCTCCGGGGCCAAACAATGTTTTTTGAAGAAGCATCCGTGAATTATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

321

Amino Acids

36.47

Weight (kDa)

4.21

Isoelectric Point (pI)

52.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 202 - 288 2.4e-10 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 872
Acc36I ACCTGC 1 cut(s) 872
AccBSI CCGCTC 1 cut(s) 777
AccII CGCG 1 cut(s) 630
AciI CCGC 2 cut(s) 628, 777
AclWI GGATC 2 cut(s) 56, 253
AcsI RAATTY 3 cut(s) 362, 671, 834
AfiI CCNNNNNNNGG 2 cut(s) 439, 634
AgsI TTSAA 6 cut(s) 56, 206, 475, 593, 856, 938
AhlI ACTAGT 1 cut(s) 110
AjnI CCWGG 1 cut(s) 770
AluBI AGCT 4 cut(s) 49, 214, 439, 700
AluI AGCT 4 cut(s) 49, 214, 439, 700
Alw26I GTCTC 2 cut(s) 47, 673
AlwI GGATC 2 cut(s) 56, 253
AoxI GGCC 2 cut(s) 368, 919
ApeKI GCWGC 2 cut(s) 809, 812
ApoI RAATTY 3 cut(s) 362, 671, 834
AseI ATTAAT 1 cut(s) 510
AspLEI GCGC 2 cut(s) 253, 632
AspS9I GGNCC 3 cut(s) 368, 601, 919
AsuC2I CCSGG 4 cut(s) 23, 123, 243, 917
AsuHPI GGTGA 5 cut(s) 71, 527, 542, 791, 920
AsuII TTCGAA 1 cut(s) 71
AvaII GGWCC 1 cut(s) 601
BbsI GAAGAC 1 cut(s) 277
BbvI GCAGC 2 cut(s) 796, 799
BccI CCATC 3 cut(s) 455, 475, 605
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 1 cut(s) 772
BcnI CCSGG 4 cut(s) 23, 123, 243, 917
BcoDI GTCTC 2 cut(s) 47, 673
BcuI ACTAGT 1 cut(s) 110
BfaI CTAG 4 cut(s) 111, 387, 434, 530
BfmI CTRYAG 1 cut(s) 813
BfuAI ACCTGC 1 cut(s) 872
BglI GCCNNNNNGGC 1 cut(s) 105
BisI GCNGC 2 cut(s) 810, 813
BlsI GCNGC 2 cut(s) 811, 814
Bme1390I CCNGG 5 cut(s) 23, 123, 243, 772, 917
Bme18I GGWCC 1 cut(s) 601
BmgT120I GGNCC 3 cut(s) 368, 601, 919
BmiI GGNNCC 2 cut(s) 486, 920
BmrFI CCNGG 5 cut(s) 23, 123, 243, 772, 917
BmsI GCATC 3 cut(s) 145, 287, 953
BpiI GAAGAC 1 cut(s) 277
BplI GAGNNNNNCTC 2 cut(s) 897, 929
BpmI CTGGAG 1 cut(s) 29
Bpu10I CCTNAGC 1 cut(s) 255
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 4 cut(s) 23, 123, 243, 917
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 4 cut(s) 122, 604, 738, 916
BsaXI ACNNNNNCTCC 2 cut(s) 752, 782
Bsc4I CCNNNNNNNGG 2 cut(s) 439, 634
BseBI CCWGG 1 cut(s) 772
BseDI CCNNGG 4 cut(s) 122, 604, 738, 916
BseGI GGATG 2 cut(s) 31, 944
BseLI CCNNNNNNNGG 2 cut(s) 439, 634
BseMII CTCAG 2 cut(s) 105, 246
BseXI GCAGC 2 cut(s) 796, 799
BseYI CCCAGC 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 630
BshFI GGCC 2 cut(s) 370, 921
BsiSI CCGG 4 cut(s) 23, 122, 242, 916
BslFI GGGAC 1 cut(s) 476
BslI CCNNNNNNNGG 2 cut(s) 439, 634
BsmAI GTCTC 2 cut(s) 47, 673
BsmFI GGGAC 1 cut(s) 476
BsnI GGCC 2 cut(s) 370, 921
Bso31I GGTCTC 1 cut(s) 47
Bsp119I TTCGAA 1 cut(s) 71
Bsp143I GATC 6 cut(s) 61, 245, 532, 547, 790, 896
Bsp19I CCATGG 1 cut(s) 738
BspACI CCGC 2 cut(s) 628, 777
BspANI GGCC 2 cut(s) 370, 921
BspCNI CTCAG 2 cut(s) 104, 247
BspFNI CGCG 1 cut(s) 630
BspLI GGNNCC 2 cut(s) 486, 920
BspMAI CTGCAG 1 cut(s) 817
BspMI ACCTGC 1 cut(s) 872
BspPI GGATC 2 cut(s) 56, 253
BspQI GCTCTTC 2 cut(s) 204, 207
BspT104I TTCGAA 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 47
BsrBI CCGCTC 1 cut(s) 777
BssECI CCNNGG 4 cut(s) 122, 604, 738, 916
BssMI GATC 6 cut(s) 61, 245, 532, 547, 790, 896
BssT1I CCWWGG 2 cut(s) 604, 738
Bst2UI CCWGG 1 cut(s) 772
Bst4CI ACNGT 1 cut(s) 620
Bst6I CTCTTC 2 cut(s) 204, 207
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 276, 441
BstDEI CTNAG 2 cut(s) 91, 255
BstDSI CCRYGG 1 cut(s) 738
BstEII GGTNACC 1 cut(s) 908
BstF5I GGATG 2 cut(s) 31, 944
BstFNI CGCG 1 cut(s) 630
BstHHI GCGC 2 cut(s) 253, 632
BstKTI GATC 6 cut(s) 64, 248, 535, 550, 793, 899
BstMAI GTCTC 2 cut(s) 47, 673
BstMBI GATC 6 cut(s) 61, 245, 532, 547, 790, 896
BstMWI GCNNNNNNNGC 2 cut(s) 105, 265
BstNI CCWGG 1 cut(s) 772
BstPI GGTNACC 1 cut(s) 908
BstSCI CCNGG 5 cut(s) 21, 121, 241, 770, 915
BstSFI CTRYAG 1 cut(s) 813
BstUI CGCG 1 cut(s) 630
BstV1I GCAGC 2 cut(s) 796, 799
BstV2I GAAGAC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 245
BstXI CCANNNNNNTGG 2 cut(s) 297, 611
BstYI RGATCY 1 cut(s) 245
BsuRI GGCC 2 cut(s) 370, 921
BtgI CCRYGG 1 cut(s) 738
BtsCI GGATG 2 cut(s) 31, 944
BveI ACCTGC 1 cut(s) 872
Cac8I GCNNGC 2 cut(s) 276, 441
CfoI GCGC 2 cut(s) 253, 632
Cfr13I GGNCC 3 cut(s) 368, 601, 919
CviAII CATG 5 cut(s) 197, 497, 668, 739, 794
DdeI CTNAG 2 cut(s) 91, 255
DpnI GATC 6 cut(s) 63, 247, 534, 549, 792, 898
DpnII GATC 6 cut(s) 61, 245, 532, 547, 790, 896
Eam1104I CTCTTC 2 cut(s) 204, 207
EarI CTCTTC 2 cut(s) 204, 207
Eco130I CCWWGG 2 cut(s) 604, 738
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 601
Eco91I GGTNACC 1 cut(s) 908
EcoO65I GGTNACC 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 770
EcoT14I CCWWGG 2 cut(s) 604, 738
ErhI CCWWGG 2 cut(s) 604, 738
FaeI CATG 5 cut(s) 200, 500, 671, 742, 797
FaqI GGGAC 1 cut(s) 476
FatI CATG 5 cut(s) 196, 496, 667, 738, 793
Fnu4HI GCNGC 2 cut(s) 810, 813
FokI GGATG 2 cut(s) 38, 931
Fsp4HI GCNGC 2 cut(s) 810, 813
FspBI CTAG 4 cut(s) 111, 387, 434, 530
GlaI GCGC 2 cut(s) 252, 631
GluI GCNGC 2 cut(s) 810, 813
GsaI CCCAGC 1 cut(s) 40
GsuI CTGGAG 1 cut(s) 29
HaeIII GGCC 2 cut(s) 370, 921
HapII CCGG 4 cut(s) 23, 122, 242, 916
HhaI GCGC 2 cut(s) 253, 632
Hin1II CATG 5 cut(s) 200, 500, 671, 742, 797
Hin6I GCGC 2 cut(s) 251, 630
HinP1I GCGC 2 cut(s) 251, 630
HinfI GANTC 2 cut(s) 89, 877
HpaII CCGG 4 cut(s) 23, 122, 242, 916
HphI GGTGA 5 cut(s) 71, 527, 542, 791, 920
Hpy188III TCNNGA 4 cut(s) 206, 330, 563, 659
HpyAV CCTTC 3 cut(s) 50, 381, 719
HpyCH4III ACNGT 1 cut(s) 620
HpyCH4V TGCA 3 cut(s) 274, 574, 815
HpyF10VI GCNNNNNNNGC 2 cut(s) 105, 265
HpyF3I CTNAG 2 cut(s) 91, 255
Hsp92II CATG 5 cut(s) 200, 500, 671, 742, 797
HspAI GCGC 2 cut(s) 251, 630
Kzo9I GATC 6 cut(s) 61, 245, 532, 547, 790, 896
LguI GCTCTTC 2 cut(s) 204, 207
LmnI GCTCC 1 cut(s) 774
Lsp1109I GCAGC 2 cut(s) 796, 799
LweI GCATC 3 cut(s) 145, 287, 953
MaeI CTAG 4 cut(s) 111, 387, 434, 530
MaeIII GTNAC 5 cut(s) 86, 216, 300, 796, 908
MalI GATC 6 cut(s) 63, 247, 534, 549, 792, 898
MbiI CCGCTC 1 cut(s) 777
MboI GATC 6 cut(s) 61, 245, 532, 547, 790, 896
MboII GAAGA 7 cut(s) 152, 194, 221, 277, 775, 872, 950
MflI RGATCY 1 cut(s) 245
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 7 cut(s) 226, 346, 462, 504, 535, 899, 923
MseI TTAA 3 cut(s) 135, 510, 831
MspI CCGG 4 cut(s) 23, 122, 242, 916
MspR9I CCNGG 5 cut(s) 23, 123, 243, 772, 917
MvaI CCWGG 1 cut(s) 772
MvnI CGCG 1 cut(s) 630
MwoI GCNNNNNNNGC 2 cut(s) 105, 265
NciI CCSGG 4 cut(s) 23, 123, 243, 917
NcoI CCATGG 1 cut(s) 738
NdeII GATC 6 cut(s) 61, 245, 532, 547, 790, 896
NlaIII CATG 5 cut(s) 200, 500, 671, 742, 797
NlaIV GGNNCC 2 cut(s) 486, 920
NmuCI GTSAC 4 cut(s) 86, 216, 300, 908
NspV TTCGAA 1 cut(s) 71
PaqCI CACCTGC 1 cut(s) 872
PciSI GCTCTTC 2 cut(s) 204, 207
PfeI GAWTC 1 cut(s) 877
PfoI TCCNGGA 1 cut(s) 770
PkrI GCNGC 2 cut(s) 811, 814
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PshBI ATTAAT 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 770
PspEI GGTNACC 1 cut(s) 908
PspFI CCCAGC 1 cut(s) 36
PspGI CCWGG 1 cut(s) 770
PspN4I GGNNCC 2 cut(s) 486, 920
PspPI GGNCC 3 cut(s) 368, 601, 919
PstI CTGCAG 1 cut(s) 817
PsuI RGATCY 1 cut(s) 245
SapI GCTCTTC 2 cut(s) 204, 207
SaqAI TTAA 3 cut(s) 135, 510, 831
SatI GCNGC 2 cut(s) 810, 813
Sau3AI GATC 6 cut(s) 61, 245, 532, 547, 790, 896
Sau96I GGNCC 3 cut(s) 368, 601, 919
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 5 cut(s) 23, 123, 243, 772, 917
SfaNI GCATC 3 cut(s) 145, 287, 953
SfcI CTRYAG 1 cut(s) 813
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 1 cut(s) 601
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
SpeI ACTAGT 1 cut(s) 110
SsiI CCGC 2 cut(s) 628, 777
SspMI CTAG 4 cut(s) 111, 387, 434, 530
StyD4I CCNGG 5 cut(s) 21, 121, 241, 770, 915
StyI CCWWGG 2 cut(s) 604, 738
TaaI ACNGT 1 cut(s) 620
TaqI TCGA 2 cut(s) 71, 899
TfiI GAWTC 1 cut(s) 877
Tru1I TTAA 3 cut(s) 135, 510, 831
Tru9I TTAA 3 cut(s) 135, 510, 831
TseFI GTSAC 4 cut(s) 86, 216, 300, 908
TseI GCWGC 2 cut(s) 809, 812
Tsp45I GTSAC 4 cut(s) 86, 216, 300, 908
TspDTI ATGAA 6 cut(s) 198, 375, 411, 462, 684, 738
TspGWI ACGGA 2 cut(s) 54, 937
VpaK11BI GGWCC 1 cut(s) 601
VspI ATTAAT 1 cut(s) 510
XapI RAATTY 3 cut(s) 362, 671, 834
XspI CTAG 4 cut(s) 111, 387, 434, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.