Rorug05G0312300

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001571
Physical Location & Seq
Reverse (-)
36896114 .. 36897371
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0312300.1

Sequence Viewer

Length: 795 bp
ATGGGTCTGCAACCATACTCTTATTATAAAAAATCTTCAGTTTGGCTAGGTCTTAAGAAACTATGGCCAACATTGCTAGAGGGAGTTCAATGGCTAGTTGGCGATGGTAAAAGTATTAGATTTTGGCGTGACAATTGGTTGGGGGAGACCCTTGTTACTTCACTCAATTATGATCAGGAGGTCCATGAATTATTGAATGACAAAGTGTCTTGCTTCATTACGAATGGCCAGTGGAACATACCGTCTAGCTTCTGTCGCACGTTGCCTGCTACAGCTCAGTCCATCCGCTCAATTAAGCTACCGGTAGAGCCCACCCCTGATCAGGTTGTCTGGTCTAAGACGGCGTCAGGATCATTGACGTCCAGTGAAGCTTACCACGCCCTAGACCCTCCTGCCCCTGGCCCTTTATGGTGCAAACGCATTTGGCATGGTGCAATACAACCCAGAAAGAGTGTCACTGCTTGGAAAGTCTTGCATGGAAAAATTCAAACAGATGATCTTCTTCAGAAATGTGGAAATGAAACATTTTCCTTTCCCAATACCCATTTTCCGGGAAACAAACATGGCCTAAGGGAATCTGCGGGCTTGATTTTTGCCCCATCATCCCTTGCATCTACAGCGGCTCCCATATTTGGCCTATTGGGAATCTCCCCATTACGGCCCATGGCACCTCGTTTTATACCAGTCACATGGGCTCCCCCGCCGGTGAATTGGTTTAAAGCAAATTCAGATGGCTCTTTTCGTGATTCTAATCATGCTGGCTATGGTGGTATTTTTAGAAACAGTGATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

264

Amino Acids

29.29

Weight (kDa)

9.28

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 120 - 173 6.8e-07 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 27
AatII GACGTC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 667
AccBSI CCGCTC 1 cut(s) 288
AciI CCGC 4 cut(s) 286, 581, 620, 701
AclWI GGATC 1 cut(s) 358
AcoI YGGCCR 2 cut(s) 65, 226
AcsI RAATTY 2 cut(s) 483, 724
AcuI CTGAAG 2 cut(s) 21, 488
AcyI GRCGYC 2 cut(s) 344, 359
AfiI CCNNNNNNNGG 5 cut(s) 322, 398, 550, 632, 657
AflII CTTAAG 1 cut(s) 53
AgeI ACCGGT 1 cut(s) 301
AgsI TTSAA 3 cut(s) 89, 196, 488
AhdI GACNNNNNGTC 1 cut(s) 205
AjnI CCWGG 1 cut(s) 397
AluBI AGCT 4 cut(s) 249, 275, 298, 371
AluI AGCT 4 cut(s) 249, 275, 298, 371
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 1 cut(s) 358
AoxI GGCC 6 cut(s) 65, 226, 400, 565, 634, 659
ApoI RAATTY 2 cut(s) 483, 724
AsiGI ACCGGT 1 cut(s) 301
AspS9I GGNCC 3 cut(s) 181, 401, 660
AsuC2I CCSGG 1 cut(s) 552
AsuHPI GGTGA 1 cut(s) 718
AvaII GGWCC 1 cut(s) 181
AxyI CCTNAGG 1 cut(s) 569
BalI TGGCCA 2 cut(s) 67, 228
BanI GGYRCC 1 cut(s) 667
BanII GRGCYC 2 cut(s) 312, 697
BccI CCATC 4 cut(s) 98, 290, 607, 725
BceAI ACGGC 2 cut(s) 357, 674
BciT130I CCWGG 1 cut(s) 399
BclI TGATCA 2 cut(s) 172, 319
BcnI CCSGG 1 cut(s) 552
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 5 cut(s) 47, 77, 95, 246, 383
BfmI CTRYAG 2 cut(s) 270, 615
BfrI CTTAAG 1 cut(s) 53
BisI GCNGC 1 cut(s) 621
BlsI GCNGC 1 cut(s) 622
Bme1390I CCNGG 2 cut(s) 399, 552
Bme18I GGWCC 1 cut(s) 181
BmeRI GACNNNNNGTC 1 cut(s) 205
BmgT120I GGNCC 3 cut(s) 181, 401, 660
BmiI GGNNCC 3 cut(s) 624, 669, 696
BmrFI CCNGG 2 cut(s) 399, 552
BmsI GCATC 2 cut(s) 620, 778
BpuMI CCSGG 1 cut(s) 552
BsaBI GATNNNNATC 1 cut(s) 750
BsaHI GRCGYC 2 cut(s) 344, 359
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 2 cut(s) 397, 663
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 4 cut(s) 607, 637, 679, 709
Bsc4I CCNNNNNNNGG 5 cut(s) 322, 398, 550, 632, 657
Bse118I RCCGGY 2 cut(s) 301, 703
Bse1I ACTGG 3 cut(s) 229, 363, 683
Bse21I CCTNAGG 1 cut(s) 569
Bse3DI GCAATG 1 cut(s) 71
Bse8I GATNNNNATC 1 cut(s) 750
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 397, 663
BseGI GGATG 2 cut(s) 282, 602
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 5 cut(s) 322, 398, 550, 632, 657
BseMI GCAATG 1 cut(s) 71
BseMII CTCAG 1 cut(s) 290
BseNI ACTGG 3 cut(s) 229, 363, 683
BshFI GGCC 6 cut(s) 67, 228, 402, 567, 636, 661
BshNI GGYRCC 1 cut(s) 667
BshTI ACCGGT 1 cut(s) 301
BsiSI CCGG 3 cut(s) 302, 551, 704
BslI CCNNNNNNNGG 5 cut(s) 322, 398, 550, 632, 657
BsmAI GTCTC 1 cut(s) 140
BsnI GGCC 6 cut(s) 67, 228, 402, 567, 636, 661
Bso31I GGTCTC 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 312, 697
Bsp143I GATC 4 cut(s) 172, 319, 350, 496
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 4 cut(s) 286, 581, 620, 701
BspANI GGCC 6 cut(s) 67, 228, 402, 567, 636, 661
BspCNI CTCAG 1 cut(s) 289
BspLI GGNNCC 3 cut(s) 624, 669, 696
BspPI GGATC 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 667
BspTI CTTAAG 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 140
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 1 cut(s) 71
BsrFI RCCGGY 2 cut(s) 301, 703
BsrI ACTGG 3 cut(s) 229, 363, 683
BssAI RCCGGY 2 cut(s) 301, 703
BssECI CCNNGG 2 cut(s) 397, 663
BssMI GATC 4 cut(s) 172, 319, 350, 496
BssNI GRCGYC 2 cut(s) 344, 359
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 243, 785
BstACI GRCGYC 2 cut(s) 344, 359
BstAFI CTTAAG 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 267, 583, 760
BstDEI CTNAG 3 cut(s) 276, 336, 569
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 2 cut(s) 282, 602
BstKTI GATC 4 cut(s) 175, 322, 353, 499
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 4 cut(s) 172, 319, 350, 496
BstMWI GCNNNNNNNGC 4 cut(s) 73, 255, 377, 617
BstNI CCWGG 1 cut(s) 399
BstSCI CCNGG 2 cut(s) 397, 550
BstSFI CTRYAG 2 cut(s) 270, 615
BstXI CCANNNNNNTGG 1 cut(s) 690
Bsu36I CCTNAGG 1 cut(s) 569
BsuRI GGCC 6 cut(s) 67, 228, 402, 567, 636, 661
BtgI CCRYGG 1 cut(s) 663
BtgZI GCGATG 1 cut(s) 117
BtsCI GGATG 2 cut(s) 282, 602
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 4 cut(s) 236, 370, 456, 790
Cac8I GCNNGC 3 cut(s) 267, 583, 760
Cfr10I RCCGGY 2 cut(s) 301, 703
Cfr13I GGNCC 3 cut(s) 181, 401, 660
CseI GACGC 1 cut(s) 333
CspAI ACCGGT 1 cut(s) 301
CviAII CATG 7 cut(s) 185, 428, 476, 563, 664, 690, 755
DdeI CTNAG 3 cut(s) 276, 336, 569
DpnI GATC 4 cut(s) 174, 321, 352, 498
DpnII GATC 4 cut(s) 172, 319, 350, 496
DraI TTTAAA 1 cut(s) 718
DriI GACNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 2 cut(s) 65, 226
Eam1105I GACNNNNNGTC 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 663
Eco24I GRGCYC 2 cut(s) 312, 697
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 1 cut(s) 181
Eco57I CTGAAG 2 cut(s) 21, 488
Eco81I CCTNAGG 1 cut(s) 569
EcoRII CCWGG 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 663
EcoT22I ATGCAT 1 cut(s) 793
EcoT38I GRGCYC 2 cut(s) 312, 697
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 7 cut(s) 188, 431, 479, 566, 667, 693, 758
FatI CATG 7 cut(s) 184, 427, 475, 562, 663, 689, 754
FauI CCCGC 2 cut(s) 574, 708
FbaI TGATCA 2 cut(s) 172, 319
Fnu4HI GCNGC 1 cut(s) 621
FokI GGATG 2 cut(s) 269, 589
FriOI GRGCYC 2 cut(s) 312, 697
Fsp4HI GCNGC 1 cut(s) 621
FspBI CTAG 5 cut(s) 47, 77, 95, 246, 383
GluI GCNGC 1 cut(s) 621
HaeIII GGCC 6 cut(s) 67, 228, 402, 567, 636, 661
HapII CCGG 3 cut(s) 302, 551, 704
HgaI GACGC 1 cut(s) 333
Hin1I GRCGYC 2 cut(s) 344, 359
Hin1II CATG 7 cut(s) 188, 431, 479, 566, 667, 693, 758
HindIII AAGCTT 1 cut(s) 369
HinfI GANTC 3 cut(s) 575, 645, 746
HpaII CCGG 3 cut(s) 302, 551, 704
HphI GGTGA 1 cut(s) 718
Hpy188I TCNGA 2 cut(s) 507, 730
Hpy188III TCNNGA 3 cut(s) 176, 348, 743
HpyCH4III ACNGT 2 cut(s) 243, 785
HpyCH4IV ACGT 2 cut(s) 260, 359
HpyCH4V TGCA 6 cut(s) 10, 414, 434, 475, 611, 791
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 255, 377, 617
HpyF3I CTNAG 3 cut(s) 276, 336, 569
HpySE526I ACGT 2 cut(s) 260, 359
Hsp92I GRCGYC 2 cut(s) 344, 359
Hsp92II CATG 7 cut(s) 188, 431, 479, 566, 667, 693, 758
Ksp22I TGATCA 2 cut(s) 172, 319
Kzo9I GATC 4 cut(s) 172, 319, 350, 496
LmnI GCTCC 2 cut(s) 628, 700
LweI GCATC 2 cut(s) 620, 778
MaeI CTAG 5 cut(s) 47, 77, 95, 246, 383
MaeII ACGT 2 cut(s) 260, 359
MaeIII GTNAC 4 cut(s) 128, 154, 454, 685
MalI GATC 4 cut(s) 174, 321, 352, 498
MbiI CCGCTC 1 cut(s) 288
MboI GATC 4 cut(s) 172, 319, 350, 496
MboII GAAGA 3 cut(s) 27, 491, 494
MfeI CAATTG 1 cut(s) 133
MhlI GDGCHC 2 cut(s) 312, 697
MlsI TGGCCA 2 cut(s) 67, 228
MluCI AATT 7 cut(s) 133, 166, 188, 291, 483, 709, 724
MluNI TGGCCA 2 cut(s) 67, 228
MnlI CCTC 4 cut(s) 73, 172, 399, 681
Mox20I TGGCCA 2 cut(s) 67, 228
Mph1103I ATGCAT 1 cut(s) 793
MscI TGGCCA 2 cut(s) 67, 228
MseI TTAA 3 cut(s) 54, 294, 717
Msp20I TGGCCA 2 cut(s) 67, 228
MspA1I CMGCKG 1 cut(s) 620
MspCI CTTAAG 1 cut(s) 53
MspI CCGG 3 cut(s) 302, 551, 704
MspR9I CCNGG 2 cut(s) 399, 552
MunI CAATTG 1 cut(s) 133
MvaI CCWGG 1 cut(s) 399
MwoI GCNNNNNNNGC 4 cut(s) 73, 255, 377, 617
NciI CCSGG 1 cut(s) 552
NcoI CCATGG 1 cut(s) 663
NdeII GATC 4 cut(s) 172, 319, 350, 496
NlaIII CATG 7 cut(s) 188, 431, 479, 566, 667, 693, 758
NlaIV GGNNCC 3 cut(s) 624, 669, 696
NmuCI GTSAC 3 cut(s) 128, 454, 685
NsiI ATGCAT 1 cut(s) 793
PfeI GAWTC 3 cut(s) 575, 645, 746
PflFI GACNNNGTC 1 cut(s) 343
PfoI TCCNGGA 1 cut(s) 550
PinAI ACCGGT 1 cut(s) 301
PkrI GCNGC 1 cut(s) 622
PsiI TTATAA 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 397
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 3 cut(s) 624, 669, 696
PspPI GGNCC 3 cut(s) 181, 401, 660
PsyI GACNNNGTC 1 cut(s) 343
SaqAI TTAA 3 cut(s) 54, 294, 717
SatI GCNGC 1 cut(s) 621
Sau3AI GATC 4 cut(s) 172, 319, 350, 496
Sau96I GGNCC 3 cut(s) 181, 401, 660
ScrFI CCNGG 2 cut(s) 399, 552
SduI GDGCHC 2 cut(s) 312, 697
SfaNI GCATC 2 cut(s) 620, 778
SfcI CTRYAG 2 cut(s) 270, 615
SgrAI CRCCGGYG 1 cut(s) 703
SinI GGWCC 1 cut(s) 181
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
Sse9I AATT 7 cut(s) 133, 166, 188, 291, 483, 709, 724
SsiI CCGC 4 cut(s) 286, 581, 620, 701
SspMI CTAG 5 cut(s) 47, 77, 95, 246, 383
StyD4I CCNGG 2 cut(s) 397, 550
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 2 cut(s) 243, 785
TaiI ACGT 2 cut(s) 263, 362
TasI AATT 7 cut(s) 133, 166, 188, 291, 483, 709, 724
TauI GCSGC 1 cut(s) 623
TfiI GAWTC 3 cut(s) 575, 645, 746
Tru1I TTAA 3 cut(s) 54, 294, 717
Tru9I TTAA 3 cut(s) 54, 294, 717
TscAI CASTG 4 cut(s) 236, 370, 463, 790
TseFI GTSAC 3 cut(s) 128, 454, 685
Tsp45I GTSAC 3 cut(s) 128, 454, 685
TspDTI ATGAA 3 cut(s) 201, 205, 534
TspRI CASTG 4 cut(s) 236, 370, 463, 790
Tth111I GACNNNGTC 1 cut(s) 343
Vha464I CTTAAG 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 181
XapI RAATTY 2 cut(s) 483, 724
XspI CTAG 5 cut(s) 47, 77, 95, 246, 383
ZraI GACGTC 1 cut(s) 360
Zsp2I ATGCAT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.