AT5G12350

(E 0.0) Ran GTPase binding chromatin binding zinc ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003738
Physical Location & Seq
Forward (+)
3995310 .. 4000801
5492 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12350.1

Sequence Viewer

Length: 3228 bp
ATGTCGAGAAACGGAAGGATGGCTTCGGATCTTAGTAGAGCTGGTCCTGTGGAGAGAGATATCGAGCAGGCTATCATTGCCCTGAAAAAGGGAGCTTACTTGCTAAAGTATGGAAGAAGAGGGAAACCTAAGTTCTGCCCATTTCGCCTTTCTAATGATGAAACTGTTTTGATATGGTTCTCTGGAAACGAGGAGAAACATCTGAAGCTGAGCCATGTTTCTAGGATCATATCTGGACAACGCACTCCTATTTTTCAGAGGTATCCTCGTCCCGAGAAGGAATATCAGTCGTTTTCACTAATATATAGTGAGAGGTCTTTGGATGTGATATGTAAGGATAAAGATGAGGCTGAGGTGTGGTTTACTGGTCTTAAGGCCTTGATTTCGCATTGCCATCAAAGAAACAGGAGAACTGAATCAAGAAGTGACGGTACACCATCTGAAGCTAATAGCCCAAGAACATATACCCGGAGAAGCTCTCCTTTACATTCTCCATTTAGTAGCAATGACAGTTTGCAGAAGGATGGTTCTAATCACCTTCGTATTCATAGTCCATTTGAGAGCCCGCCTAAGAATGGCCTCGACAAGGCATTTTCGGACATGGCTTTATATGCAGTTCCTCCAAAAGGATTTTACCCCTCAGATTCTGCAACTATTTCTGTTCATTCTGGAGGCTCGGATAGCATGCATGGACATATGAGGGGTATGGGCATGGATGCCTTTAGAGTTAGTATGTCAAGTGCTGTTAGTTCCTCGAGCCATGGTTCTGGTCATGATGATGGAGATGCATTAGGAGATGTTTTCATCTGGGGAGAAGGCATAGGAGAAGGTGTTTTGGGTGGTGGAAACCGTAGAGTTGGTAGTTCATTTGATATAAAAATGGATTCCTTATTGCCAAAAGCTTTAGAATCTACAATAGTACTGGACGTCCAAAATATTGCTTGTGGTGGACAGCACGCTGTCCTCGTGACAAAACAAGGGGAAAGTTTTTCTTGGGGAGAGGAATCTGAAGGCAGGCTTGGCCACGGTGTTGATTCCAATATTCAACAACCAAAGCTCATCGATGCACTCAACACCACAAATATTGAGCTTGTAGCATGTGGTGAATTTCATAGTTGTGCAGTTACCCTATCAGGGGATTTGTATACCTGGGGTAAAGGAGATTTTGGTGTTCTTGGACATGGAAATGAAGTAAGTCACTGGGTCCCTAAGAGGGTTAATTTTCTGCTGGAAGGGATACATGTATCATCTATTGCTTGTGGACCTTACCACACAGCAGTCGTTACCTCTGCTGGGCAGTTGTTTACTTTTGGTGATGGGACCTTTGGTGTTTTAGGCCACGGAGACAAGAAAAGTGTTTTCATACCTCGGGAGGTAGACTCTTTGAAAGGTCTTCGCACTGTCCGGGCAGCTTGTGGTGTATGGCACACAGCAGCAGTTGTGGAAGTAATGGTTGGGAGCTCAAGCTCAAGTAATTGCTCTTCTGGAAAGCTCTTTACATGGGGTGATGGTGATAAGGGTCGTCTTGGTCATGGTAATAAAGAACCAAAACTTGTGCCTACCTGTGTTGCTGCTCTGGTTGAACCCAATTTTTGCCAAGTTGCATGTGGGCACAGTCTAACGGTTGCACTTACAACATCAGGCCATGTCTATACTATGGGCAGTCCTGTCTATGGTCAGCTTGGAAACTCACATGCAGACGGAAAAACTCCAAACCGTGTCGAAGGTAAACTTCACAAGAGTTTTGTCGAAGAGATAGCCTGTGGTGCTTATCATGTTGCAGTTTTAACTTCGAGGACTGAGGTTTACACTTGGGGAAAGGGATCAAATGGTAGACTTGGTCATGGAGATGTAGATGATAGAAATTCCCCGACATTGGTTGAGTCACTTAAGGATAAACAGGTGAAAAGTATTGCCTGTGGCACTAACTTCACAGCAGCTGTCTGCATTCACAGGTGGGCATCAGGGATGGACCAGTCCATGTGTTCAGGTTGCCGTCAGCCCTTCAGTTTTAAAAGAAAGAGGCACAATTGCTATAATTGCGGACTAGTGTTTTGCCACTCGTGCACTAGTAAAAAGTCATTGAAGGCTTGTATGGCACCGAACCCCAACAAACCATATCGAGTGTGTGATAAGTGTTTTAACAAATTGAAAAAGACCATGGAAACAGATCCATCATCCCATTCTTCTCTGAGTAGAAGAGGAAGCATTAACCAGGGATCAGATCCCATTGACAAAGATGACAAGTTCGATTCTAGATCCGATGGACAGTTAGCTAGATTTTCATTAATGGAATCCATGAGACAAGTGGACAGTCGACATAAGAAGAACAAGAAGTATGAATTCAATAGTAGTCGTGTCTCACCCATACCGAGTGGAAGCTCTCAACGGGGTGCACTTAACATAGCCAAGTCTTTCAATCCAGTATTTGGAGCATCAAAGAAGTTCTTCTCAGCTTCTGTTCCTGGTTCTCGAATTGTGTCTCGGGCAACTTCCCCAATATCGAGGCGTCCTAGTCCACCCCGGTCAACTACACCGACTCCCACTCTTTCAGGACTAGCCACACCAAAATTTGTAGTGGATGATACTAAGAGAACCAATGATAACCTAAGCCAAGAGGTCGTTAAGCTAAGATCTCAGGTTGAAAGTCTTACAAGGAAGGCCCAGCTTCAAGAAGTTGAGCTGGAAAGAACAACCAAGCAGCTAAAAGAGGCGTTGGCAATAACTAATGAAGAAACGACAAGATGCAAGGCAGCAAAAGAAGTGATCAAATCACTTACCGCTCAATTAAAAGACATGGCCGAAAGATTACCCGTTGGATCAGCTCGGACTGTGAAGTCTCCTCCTTCTCTTAACTCATTTGGTTCCAGCCCTGGTCGCATTGACCCTTTTAATATCTTAAACCAAGCAAATAGCCAAGAATCCGAGCCAAATGGCATCACGACCCCAATGTTTTCTAATGGGACCATGACACCTGCATTTGGGAATGGTGAAGCAACGAATGAAGCACGGAACGAGAAGGAATGGGTTGAACAAGATGAACCTGGTGTCTATATCACTCTTACAGCCTTAGCCGGAGGTGCTAGAGATCTCAAACGCGTCCGTTTCAGCCGAAAAAGGTTTAGTGAGATACAAGCAGAACAGTGGTGGGCAGATAACAGAGGAAGAGTCTATGAACAATACAATGTTCGCATGGTTGACAAAGCCAGTGAGGACTTGCCTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N FYVE PH_12 RCC1

Protein Analysis

1075

Amino Acids

117.52

Weight (kDa)

9.13

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 22 - 128 3.2e-09 Pleckstrin homology domain
WD40_RLD PF25390 266 - 647 1e-83 RCC1-like domain
RCC1 PF00415 327 - 375 5.8e-10 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 329 - 437 1.7e-06 Alsin RCC1-like domain
RCC1_2 PF13540 364 - 390 5.4e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 378 - 428 3e-15 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 415 - 443 2.5e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 432 - 480 2.1e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 495 - 544 1.9e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 547 - 596 1.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 601 - 648 3e-15 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 652 - 718 1.7e-14 FYVE zinc finger
BRX_N PF13713 911 - 944 8.4e-16 Transcription factor BRX N-terminal domain
BRX PF08381 1006 - 1061 1.2e-27 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2983
AasI GACNNNNNNGTC 1 cut(s) 2836
AatII GACGTC 1 cut(s) 930
Acc36I ACCTGC 1 cut(s) 2983
AccB1I GGYRCC 1 cut(s) 2098
AccB7I CCANNNNNTGG 1 cut(s) 2429
AccBSI CCGCTC 1 cut(s) 2783
AccI GTMKAC 4 cut(s) 1145, 1377, 1834, 2317
AccII CGCG 1 cut(s) 3099
AciI CCGC 3 cut(s) 566, 2043, 2781
AclWI GGATC 8 cut(s) 36, 233, 1831, 2165, 2219, 2227, 2253, 2827
AcoI YGGCCR 2 cut(s) 1021, 2799
AcsI RAATTY 4 cut(s) 1106, 1864, 2342, 2570
AcuI CTGAAG 4 cut(s) 224, 462, 1029, 1990
AcyI GRCGYC 2 cut(s) 927, 2509
AfaI GTAC 2 cut(s) 433, 921
AflII CTTAAG 2 cut(s) 371, 1889
AflIII ACRYGT 2 cut(s) 1240, 3097
AhlI ACTAGT 2 cut(s) 2047, 2069
AjnI CCWGG 5 cut(s) 1148, 2214, 2464, 2872, 3043
AjuI GAANNNNNNNTTGG 4 cut(s) 1002, 1034, 1437, 1469
Alw21I GWGCWC 3 cut(s) 1463, 2069, 2398
Alw26I GTCTC 5 cut(s) 1338, 2296, 2365, 2487, 2844
Alw44I GTGCAC 2 cut(s) 2065, 2394
AlwI GGATC 8 cut(s) 36, 233, 1831, 2165, 2219, 2227, 2253, 2827
AlwNI CAGNNNCTG 3 cut(s) 647, 1940, 2459
Ama87I CYCGRG 4 cut(s) 272, 754, 1368, 2484
AoxI GGCC 7 cut(s) 375, 577, 1021, 1336, 1642, 2661, 2799
ApaLI GTGCAC 2 cut(s) 2065, 2394
ApeKI GCWGC 6 cut(s) 1409, 1433, 1571, 1937, 2701, 2753
ApoI RAATTY 4 cut(s) 1106, 1864, 2342, 2570
ArsI GACNNNNNNTTYG 2 cut(s) 2037, 2069
AseI ATTAAT 1 cut(s) 2288
Asp700I GAANNNNTTC 1 cut(s) 2447
AspS9I GGNCC 7 cut(s) 44, 1204, 1262, 1320, 1972, 2662, 2964
AsuC2I CCSGG 3 cut(s) 469, 1406, 2524
AsuHPI GGTGA 8 cut(s) 527, 1115, 1325, 1517, 1523, 1915, 2355, 3002
AvaI CYCGRG 4 cut(s) 272, 754, 1368, 2484
AvaII GGWCC 6 cut(s) 44, 1204, 1262, 1320, 1972, 2964
BaeGI GKGCMC 3 cut(s) 1614, 2069, 2398
BalI TGGCCA 1 cut(s) 1023
BanI GGYRCC 1 cut(s) 2098
BanII GRGCYC 2 cut(s) 566, 1463
BarI GAAGNNNNNNTAC 4 cut(s) 415, 447, 1465, 1497
BauI CACGAG 2 cut(s) 965, 2062
BbsI GAAGAC 1 cut(s) 1385
Bbv12I GWGCWC 3 cut(s) 1463, 2069, 2398
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 6 cut(s) 1421, 1445, 1558, 1949, 2713, 2765
BceAI ACGGC 1 cut(s) 1980
BciT130I CCWGG 5 cut(s) 1150, 2216, 2466, 2874, 3045
BciVI GTATCC 2 cut(s) 273, 1230
BclI TGATCA 1 cut(s) 2766
BcnI CCSGG 3 cut(s) 469, 1406, 2524
BcoDI GTCTC 5 cut(s) 1338, 2296, 2365, 2487, 2844
BcuI ACTAGT 2 cut(s) 2047, 2069
BfaI CTAG 8 cut(s) 222, 2048, 2070, 2256, 2277, 2514, 2558, 3084
BfrI CTTAAG 2 cut(s) 371, 1889
BfuAI ACCTGC 1 cut(s) 2983
BfuI GTATCC 2 cut(s) 273, 1230
BglII AGATCT 2 cut(s) 2633, 3088
BisI GCNGC 6 cut(s) 1410, 1434, 1572, 1938, 2702, 2754
BlpI GCTNAGC 1 cut(s) 209
BlsI GCNGC 6 cut(s) 1411, 1435, 1573, 1939, 2703, 2755
BmcAI AGTACT 1 cut(s) 921
Bme1390I CCNGG 8 cut(s) 469, 1150, 1406, 2216, 2466, 2524, 2874, 3045
Bme18I GGWCC 6 cut(s) 44, 1204, 1262, 1320, 1972, 2964
BmeT110I CYCGRG 4 cut(s) 272, 754, 1368, 2484
BmgT120I GGNCC 7 cut(s) 44, 1204, 1262, 1320, 1972, 2662, 2964
BmiI GGNNCC 6 cut(s) 1205, 1206, 1321, 2100, 2866, 2965
BmrFI CCNGG 8 cut(s) 469, 1150, 1406, 2216, 2466, 2524, 2874, 3045
BmrI ACTGGG 1 cut(s) 1210
BmsI GCATC 7 cut(s) 706, 775, 1054, 1970, 2444, 2735, 2946
BmuI ACTGGG 1 cut(s) 1210
BpiI GAAGAC 1 cut(s) 1385
BplI GAGNNNNNCTC 6 cut(s) 250, 282, 463, 495, 1364, 1396
BpmI CTGGAG 1 cut(s) 690
Bpu10I CCTNAGC 3 cut(s) 351, 2609, 3070
Bpu1102I GCTNAGC 1 cut(s) 209
BpuEI CTTGAG 2 cut(s) 1447, 1453
BpuMI CCSGG 3 cut(s) 469, 1406, 2524
Bsa29I ATCGAT 1 cut(s) 1062
BsaHI GRCGYC 2 cut(s) 927, 2509
BsaJI CCNNGG 9 cut(s) 760, 1024, 1149, 1339, 1367, 2160, 2215, 2522, 2872
BsaXI ACNNNNNCTCC 4 cut(s) 816, 846, 2524, 2554
Bse1I ACTGG 6 cut(s) 370, 927, 1205, 1975, 2423, 3207
Bse3DI GCAATG 3 cut(s) 75, 388, 511
BseBI CCWGG 5 cut(s) 1150, 2216, 2466, 2874, 3045
BseCI ATCGAT 1 cut(s) 1062
BseDI CCNNGG 9 cut(s) 760, 1024, 1149, 1339, 1367, 2160, 2215, 2522, 2872
BseGI GGATG 7 cut(s) 24, 328, 529, 721, 1974, 2177, 2587
BseMI GCAATG 3 cut(s) 75, 388, 511
BseMII CTCAG 7 cut(s) 200, 342, 654, 1791, 2183, 2466, 2651
BseNI ACTGG 6 cut(s) 370, 927, 1205, 1975, 2423, 3207
BseRI GAGGAG 2 cut(s) 206, 2832
BseSI GKGCMC 3 cut(s) 1614, 2069, 2398
BseXI GCAGC 6 cut(s) 1421, 1445, 1558, 1949, 2713, 2765
BseYI CCCAGC 2 cut(s) 1292, 2664
BsgI GTGCAG 1 cut(s) 1140
Bsh1236I CGCG 1 cut(s) 3099
BshFI GGCC 7 cut(s) 377, 579, 1023, 1338, 1644, 2663, 2801
BshNI GGYRCC 1 cut(s) 2098
BshVI ATCGAT 1 cut(s) 1062
BsiHKAI GWGCWC 3 cut(s) 1463, 2069, 2398
BsiHKCI CYCGRG 4 cut(s) 272, 754, 1368, 2484
BsiSI CCGG 4 cut(s) 469, 1405, 2524, 3075
BslFI GGGAC 4 cut(s) 255, 1190, 1333, 2977
BsmAI GTCTC 5 cut(s) 1338, 2296, 2365, 2487, 2844
BsmFI GGGAC 4 cut(s) 255, 1190, 1333, 2977
BsmI GAATGC 1 cut(s) 1947
BsnI GGCC 7 cut(s) 377, 579, 1023, 1338, 1644, 2663, 2801
BsoBI CYCGRG 4 cut(s) 272, 754, 1368, 2484
Bsp1286I GDGCHC 5 cut(s) 566, 1463, 1614, 2069, 2398
Bsp1720I GCTNAGC 1 cut(s) 209
Bsp19I CCATGG 2 cut(s) 760, 2160
BspACI CCGC 3 cut(s) 566, 2043, 2781
BspANI GGCC 7 cut(s) 377, 579, 1023, 1338, 1644, 2663, 2801
BspCNI CTCAG 7 cut(s) 201, 343, 653, 1792, 2184, 2465, 2650
BspDI ATCGAT 1 cut(s) 1062
BspFNI CGCG 1 cut(s) 3099
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 6 cut(s) 1205, 1206, 1321, 2100, 2866, 2965
BspMI ACCTGC 1 cut(s) 2983
BspPI GGATC 8 cut(s) 36, 233, 1831, 2165, 2219, 2227, 2253, 2827
BspQI GCTCTTC 1 cut(s) 1486
BspT107I GGYRCC 1 cut(s) 2098
BspTI CTTAAG 2 cut(s) 371, 1889
BsrBI CCGCTC 1 cut(s) 2783
BsrDI GCAATG 3 cut(s) 75, 388, 511
BsrI ACTGG 6 cut(s) 370, 927, 1205, 1975, 2423, 3207
BssECI CCNNGG 9 cut(s) 760, 1024, 1149, 1339, 1367, 2160, 2215, 2522, 2872
BssNAI GTATAC 1 cut(s) 1146
BssNI GRCGYC 2 cut(s) 927, 2509
BssSI CACGAG 2 cut(s) 965, 2062
BssT1I CCWWGG 2 cut(s) 760, 2160
Bst1107I GTATAC 1 cut(s) 1146
Bst2BI CACGAG 2 cut(s) 965, 2062
Bst2UI CCWGG 5 cut(s) 1150, 2216, 2466, 2874, 3045
Bst6I CTCTTC 5 cut(s) 112, 1486, 1746, 2194, 3160
BstACI GRCGYC 2 cut(s) 927, 2509
BstAFI CTTAAG 2 cut(s) 371, 1889
BstC8I GCNNGC 5 cut(s) 69, 566, 686, 957, 1016
BstDSI CCRYGG 4 cut(s) 760, 1024, 1339, 2160
BstENI CCTNNNNNAGG 3 cut(s) 86, 584, 624
BstF5I GGATG 7 cut(s) 24, 328, 529, 721, 1974, 2177, 2587
BstFNI CGCG 1 cut(s) 3099
BstMAI GTCTC 5 cut(s) 1338, 2296, 2365, 2487, 2844
BstNI CCWGG 5 cut(s) 1150, 2216, 2466, 2874, 3045
BstNSI RCATGY 5 cut(s) 688, 1101, 1244, 1608, 1697
BstSCI CCNGG 8 cut(s) 467, 1148, 1404, 2214, 2464, 2522, 2872, 3043
BstSLI GKGCMC 3 cut(s) 1614, 2069, 2398
BstUI CGCG 1 cut(s) 3099
BstV1I GCAGC 6 cut(s) 1421, 1445, 1558, 1949, 2713, 2765
BstV2I GAAGAC 1 cut(s) 1385
BstX2I RGATCY 6 cut(s) 28, 2170, 2224, 2258, 2633, 3088
BstXI CCANNNNNNTGG 1 cut(s) 767
BstYI RGATCY 6 cut(s) 28, 2170, 2224, 2258, 2633, 3088
BstZ17I GTATAC 1 cut(s) 1146
Bsu15I ATCGAT 1 cut(s) 1062
BsuI GTATCC 2 cut(s) 273, 1230
BsuRI GGCC 7 cut(s) 377, 579, 1023, 1338, 1644, 2663, 2801
BsuTUI ATCGAT 1 cut(s) 1062
BtgI CCRYGG 4 cut(s) 760, 1024, 1339, 2160
BtsCI GGATG 7 cut(s) 24, 328, 529, 721, 1974, 2177, 2587
BtsIMutI CAGTG 4 cut(s) 1198, 1398, 3149, 3214
BveI ACCTGC 1 cut(s) 2983
Cac8I GCNNGC 5 cut(s) 69, 566, 686, 957, 1016
CaiI CAGNNNCTG 3 cut(s) 647, 1940, 2459
CciI TCATGA 1 cut(s) 772
Cfr13I GGNCC 7 cut(s) 44, 1204, 1262, 1320, 1972, 2662, 2964
ClaI ATCGAT 1 cut(s) 1062
CseI GACGC 2 cut(s) 2498, 3088
CsiI ACCWGGT 1 cut(s) 3043
Csp6I GTAC 2 cut(s) 432, 920
CspCI CAANNNNNGTGG 2 cut(s) 1066, 1101
CviQI GTAC 2 cut(s) 432, 920
DraI TTTAAA 1 cut(s) 2014
DrdI GACNNNNNNGTC 1 cut(s) 2836
DseDI GACNNNNNNGTC 1 cut(s) 2836
EaeI YGGCCR 2 cut(s) 1021, 2799
Eam1104I CTCTTC 5 cut(s) 112, 1486, 1746, 2194, 3160
EarI CTCTTC 5 cut(s) 112, 1486, 1746, 2194, 3160
Ecl136II GAGCTC 1 cut(s) 1461
Eco130I CCWWGG 2 cut(s) 760, 2160
Eco147I AGGCCT 1 cut(s) 377
Eco24I GRGCYC 2 cut(s) 566, 1463
Eco32I GATATC 1 cut(s) 61
Eco47I GGWCC 6 cut(s) 44, 1204, 1262, 1320, 1972, 2964
Eco53kI GAGCTC 1 cut(s) 1461
Eco57I CTGAAG 4 cut(s) 224, 462, 1029, 1990
Eco88I CYCGRG 4 cut(s) 272, 754, 1368, 2484
EcoICRI GAGCTC 1 cut(s) 1461
EcoNI CCTNNNNNAGG 3 cut(s) 86, 584, 624
EcoO109I RGGNCCY 2 cut(s) 1204, 1320
EcoRI GAATTC 1 cut(s) 2342
EcoRII CCWGG 5 cut(s) 1148, 2214, 2464, 2872, 3043
EcoRV GATATC 1 cut(s) 61
EcoT14I CCWWGG 2 cut(s) 760, 2160
EcoT22I ATGCAT 2 cut(s) 690, 790
EcoT38I GRGCYC 2 cut(s) 566, 1463
ErhI CCWWGG 2 cut(s) 760, 2160
FaqI GGGAC 4 cut(s) 255, 1190, 1333, 2977
FauI CCCGC 1 cut(s) 573
FauNDI CATATG 1 cut(s) 696
FbaI TGATCA 1 cut(s) 2766
FblI GTMKAC 4 cut(s) 1145, 1377, 1834, 2317
Fnu4HI GCNGC 6 cut(s) 1410, 1434, 1572, 1938, 2702, 2754
FokI GGATG 7 cut(s) 31, 335, 536, 728, 1981, 2164, 2594
FriOI GRGCYC 2 cut(s) 566, 1463
Fsp4HI GCNGC 6 cut(s) 1410, 1434, 1572, 1938, 2702, 2754
FspBI CTAG 8 cut(s) 222, 2048, 2070, 2256, 2277, 2514, 2558, 3084
GluI GCNGC 6 cut(s) 1410, 1434, 1572, 1938, 2702, 2754
GsaI CCCAGC 2 cut(s) 1296, 2668
GsuI CTGGAG 1 cut(s) 690
HaeIII GGCC 7 cut(s) 377, 579, 1023, 1338, 1644, 2663, 2801
HapII CCGG 4 cut(s) 469, 1405, 2524, 3075
HgaI GACGC 2 cut(s) 2498, 3088
Hin1I GRCGYC 2 cut(s) 927, 2509
HincII GTYRAC 3 cut(s) 2318, 2529, 3199
HindII GTYRAC 3 cut(s) 2318, 2529, 3199
HindIII AAGCTT 1 cut(s) 900
HpaII CCGG 4 cut(s) 469, 1405, 2524, 3075
HphI GGTGA 8 cut(s) 527, 1115, 1325, 1517, 1523, 1915, 2355, 3002
HpyCH4IV ACGT 1 cut(s) 927
HpySE526I ACGT 1 cut(s) 927
Hsp92I GRCGYC 2 cut(s) 927, 2509
KflI GGGWCCC 1 cut(s) 1204
Ksp22I TGATCA 1 cut(s) 2766
LguI GCTCTTC 1 cut(s) 1486
LmnI GCTCC 3 cut(s) 92, 1458, 2432
Lsp1109I GCAGC 6 cut(s) 1421, 1445, 1558, 1949, 2713, 2765
LweI GCATC 7 cut(s) 706, 775, 1054, 1970, 2444, 2735, 2946
MabI ACCWGGT 1 cut(s) 3043
MaeI CTAG 8 cut(s) 222, 2048, 2070, 2256, 2277, 2514, 2558, 3084
MaeII ACGT 1 cut(s) 927
MaeIII GTNAC 6 cut(s) 425, 967, 1123, 1196, 1282, 1884
MbiI CCGCTC 1 cut(s) 2783
MfeI CAATTG 1 cut(s) 2029
MflI RGATCY 6 cut(s) 28, 2170, 2224, 2258, 2633, 3088
MhlI GDGCHC 5 cut(s) 566, 1463, 1614, 2069, 2398
MlsI TGGCCA 1 cut(s) 1023
MluI ACGCGT 1 cut(s) 3097
MluNI TGGCCA 1 cut(s) 1023
MlyI GAGTC 4 cut(s) 1373, 1892, 2533, 3177
MmeI TCCRAC 1 cut(s) 2797
Mox20I TGGCCA 1 cut(s) 1023
Mph1103I ATGCAT 2 cut(s) 690, 790
MroXI GAANNNNTTC 1 cut(s) 2447
MscI TGGCCA 1 cut(s) 1023
MslI CAYNNNNRTG 4 cut(s) 777, 1116, 1185, 1848
Msp20I TGGCCA 1 cut(s) 1023
MspA1I CMGCKG 1 cut(s) 1940
MspCI CTTAAG 2 cut(s) 371, 1889
MspI CCGG 4 cut(s) 469, 1405, 2524, 3075
MspR9I CCNGG 8 cut(s) 469, 1150, 1406, 2216, 2466, 2524, 2874, 3045
MunI CAATTG 1 cut(s) 2029
Mva1269I GAATGC 1 cut(s) 1947
MvaI CCWGG 5 cut(s) 1150, 2216, 2466, 2874, 3045
MvnI CGCG 1 cut(s) 3099
NciI CCSGG 3 cut(s) 469, 1406, 2524
NcoI CCATGG 2 cut(s) 760, 2160
NdeI CATATG 1 cut(s) 696
NlaIV GGNNCC 6 cut(s) 1205, 1206, 1321, 2100, 2866, 2965
NmuCI GTSAC 4 cut(s) 425, 967, 1196, 1884
NsiI ATGCAT 2 cut(s) 690, 790
NspI RCATGY 5 cut(s) 688, 1101, 1244, 1608, 1697
PaeI GCATGC 1 cut(s) 688
PaeR7I CTCGAG 1 cut(s) 754
PagI TCATGA 1 cut(s) 772
PaqCI CACCTGC 1 cut(s) 2983
PceI AGGCCT 1 cut(s) 377
PciI ACATGT 1 cut(s) 1240
PciSI GCTCTTC 1 cut(s) 1486
PcsI WCGNNNNNNNCGW 1 cut(s) 963
PctI GAATGC 1 cut(s) 1947
PdmI GAANNNNTTC 1 cut(s) 2447
PfeI GAWTC 9 cut(s) 416, 644, 884, 908, 1004, 1034, 2252, 2294, 2921
PflFI GACNNNGTC 1 cut(s) 1839
PflMI CCANNNNNTGG 1 cut(s) 2429
PkrI GCNGC 6 cut(s) 1411, 1435, 1573, 1939, 2703, 2755
PleI GAGTC 4 cut(s) 1373, 1891, 2533, 3176
PpsI GAGTC 4 cut(s) 1373, 1891, 2533, 3176
PpuMI RGGWCCY 2 cut(s) 1204, 1320
PscI ACATGT 1 cut(s) 1240
PshBI ATTAAT 1 cut(s) 2288
Psp124BI GAGCTC 1 cut(s) 1463
Psp5II RGGWCCY 2 cut(s) 1204, 1320
Psp6I CCWGG 5 cut(s) 1148, 2214, 2464, 2872, 3043
PspFI CCCAGC 2 cut(s) 1292, 2664
PspGI CCWGG 5 cut(s) 1148, 2214, 2464, 2872, 3043
PspN4I GGNNCC 6 cut(s) 1205, 1206, 1321, 2100, 2866, 2965
PspPI GGNCC 7 cut(s) 44, 1204, 1262, 1320, 1972, 2662, 2964
PspPPI RGGWCCY 2 cut(s) 1204, 1320
PspXI VCTCGAGB 1 cut(s) 754
PstNI CAGNNNCTG 3 cut(s) 647, 1940, 2459
PsuI RGATCY 6 cut(s) 28, 2170, 2224, 2258, 2633, 3088
PsyI GACNNNGTC 1 cut(s) 1839
PvuII CAGCTG 1 cut(s) 1940
RsaI GTAC 2 cut(s) 433, 921
RsaNI GTAC 2 cut(s) 432, 920
RseI CAYNNNNRTG 4 cut(s) 777, 1116, 1185, 1848
SacI GAGCTC 1 cut(s) 1463
SalI GTCGAC 1 cut(s) 2316
SapI GCTCTTC 1 cut(s) 1486
SatI GCNGC 6 cut(s) 1410, 1434, 1572, 1938, 2702, 2754
Sau96I GGNCC 7 cut(s) 44, 1204, 1262, 1320, 1972, 2662, 2964
ScaI AGTACT 1 cut(s) 921
SchI GAGTC 4 cut(s) 1373, 1892, 2533, 3177
ScrFI CCNGG 8 cut(s) 469, 1150, 1406, 2216, 2466, 2524, 2874, 3045
SduI GDGCHC 5 cut(s) 566, 1463, 1614, 2069, 2398
SexAI ACCWGGT 1 cut(s) 3043
SfaNI GCATC 7 cut(s) 706, 775, 1054, 1970, 2444, 2735, 2946
Sfr274I CTCGAG 1 cut(s) 754
SinI GGWCC 6 cut(s) 44, 1204, 1262, 1320, 1972, 2964
SlaI CTCGAG 1 cut(s) 754
SmiMI CAYNNNNRTG 4 cut(s) 777, 1116, 1185, 1848
SmlI CTYRAG 5 cut(s) 371, 754, 1462, 1468, 1889
SmoI CTYRAG 5 cut(s) 371, 754, 1462, 1468, 1889
SpeI ACTAGT 2 cut(s) 2047, 2069
SphI GCATGC 1 cut(s) 688
SseBI AGGCCT 1 cut(s) 377
SsiI CCGC 3 cut(s) 566, 2043, 2781
SspI AATATT 3 cut(s) 937, 1042, 1084
SspMI CTAG 8 cut(s) 222, 2048, 2070, 2256, 2277, 2514, 2558, 3084
SstI GAGCTC 1 cut(s) 1463
StuI AGGCCT 1 cut(s) 377
StyD4I CCNGG 8 cut(s) 467, 1148, 1404, 2214, 2464, 2522, 2872, 3043
StyI CCWWGG 2 cut(s) 760, 2160
TaiI ACGT 1 cut(s) 930
TatI WGTACW 1 cut(s) 919
TfiI GAWTC 9 cut(s) 416, 644, 884, 908, 1004, 1034, 2252, 2294, 2921
TscAI CASTG 4 cut(s) 1205, 1405, 3149, 3214
TseFI GTSAC 4 cut(s) 425, 967, 1196, 1884
TseI GCWGC 6 cut(s) 1409, 1433, 1571, 1937, 2701, 2753
Tsp45I GTSAC 4 cut(s) 425, 967, 1196, 1884
TspGWI ACGGA 5 cut(s) 27, 1356, 1716, 3025, 3092
TspRI CASTG 4 cut(s) 1205, 1405, 3149, 3214
Tth111I GACNNNGTC 1 cut(s) 1839
Van91I CCANNNNNTGG 1 cut(s) 2429
Vha464I CTTAAG 2 cut(s) 371, 1889
VneI GTGCAC 2 cut(s) 2065, 2394
VpaK11BI GGWCC 6 cut(s) 44, 1204, 1262, 1320, 1972, 2964
VspI ATTAAT 1 cut(s) 2288
XagI CCTNNNNNAGG 3 cut(s) 86, 584, 624
XapI RAATTY 4 cut(s) 1106, 1864, 2342, 2570
XbaI TCTAGA 1 cut(s) 2255
XceI RCATGY 5 cut(s) 688, 1101, 1244, 1608, 1697
XcmI CCANNNNNNNNNTGG 2 cut(s) 1604, 2305
XhoI CTCGAG 1 cut(s) 754
XmiI GTMKAC 4 cut(s) 1145, 1377, 1834, 2317
XmnI GAANNNNTTC 1 cut(s) 2447
XspI CTAG 8 cut(s) 222, 2048, 2070, 2256, 2277, 2514, 2558, 3084
ZraI GACGTC 1 cut(s) 928
ZrmI AGTACT 1 cut(s) 921
Zsp2I ATGCAT 2 cut(s) 690, 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.