pycom07g02670

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003738
Physical Location & Seq
Reverse (-)
2197794 .. 2204908
7115 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g02670.1

Sequence Viewer

Length: 3255 bp
ATGCTGCGTATGGACAGGATGACTGCGGATCAGGCAAAGGCGGGCCCACTCGAAAGGGACATTGACTTGGCCATCACTGCTCTGAAAAAAGGGGCACAATTGCTCAAGTATGGGCGCAGAGGAAAACCCAAATTTTGTCCTTTCAGGCTTTCCAATGATGAATCTGTTCTAATATGGTACTCAGGAAAAGAGGAGAAATGTGTTAAACTAAGCCAAGTCTTGCGAATTGTACCTGGTCAGCGCACTCAAACCTTTCAGAGGTATCCTCGGCCTGAAAAGGAATATCAGTCATTTTCTCTTATTTGTAATGATAGGTCTTTGGATTTGATATGCAAGGATAAAGATGAAGCTGAAGTTTGGTTTACTGGTCTAAAAGCATTAATATCACGTGGCCTTCATCAGAAAGGGAGACCAGAATCAAGAAGTGGAATTACATCTGAGGCAAACAGTCCGAGATCACACACCCAACAAAGTTCTCCATTGAGCTCTCCATTTTGTAGTGGTGATAGTTCGCAGAAGGATGGCGTGGAACCGTTTCATCTTCATGCTGCATTTGAAAGCCCTCCTAAAATTGGTTTAGAGAAGGCTTTATCGGATGTCATGTTATATGCCCTGCCTCCAAAAATCCCCTTCCCTTCAGATGCTGCTTTTGCCTCAGTTCCATCATCGGGAGGCTCAGATGGAATGAATGGACGAACAAAAGGTGCAGGTGTGGATGCATTTAGAGTAAGTTTATCAAGTGCTGTTAGCTCATCAAGTCAAGGCTCTGGTCATGATGAAGTTGATGCTTTAGGGGATGTTTTCATTTGGGGGGAAGGCGTTGGCGATTGCTTTCTGGGTGGAGGATCACATAGAGTTGAAAGTTCTTCTAGTTGTAAGATGGATTCTTTTGTGCCTAAAGTCTTGGAATCTGCTGTAGTACTTGATGTTCAAAGCATAGCTTGTGGTGGACGGCATGCTGCTTTTGTAACCAAGCAAGGAGAGGTATTTTCTTGGGGTGAAGAATTAGGAGGCAGACTTGGACATGGTGTGGATGCTGATGTTTCGCATCCAAAACTCATAGATGCTCTGAAAAATATGAATATTGAATTTTTAGCATGTGGGGAATACCATTCTTGTGCTGTCACACTTTCCGGTGACATGTACACATGGGGTGGCAGTACTTCCAATTTTGCGCTCCTTGGTCATGGGTTTCAATCAAGTCAATGGGTCCCAAAAAAGCTAAATGGACCTTTAGAGGGAATTCATGTCTCATCAGTTTCATGTGGACCGTGGCACACAGCTGTTGTGACCTCTGCTGGGCAATTGTTTACTTTTGGCGATGGCACATTTGGTGTTCTAGGTCATGGGGACCGAAAGAGCATTTCAGTGCCCAGGGAGGTTGATTCCCTTAAAGGTCTCCGTACTGTGCGAGTAGCTTGTGGTGTTTGGCACACTGCAGCAGTAATTGAAGTTATGGTGGGGTCATCAAGTTCCAGCAATTGCTCTTCTGGAAAGCTTTTTACATGGGGAGATGGAGATAAAGGTCGACTTGGACATGGTGACAAGGAAGCAAGACTTGTGCCCACTTGTGTTGCTTCTTTGGTTGAACCCAACTTTTGTAAAGTTGCCTGTGGACAAAGCCTGACTGTTGCACTCACAACTACGGGCCATGTTTACACTATGGGAAGCCCTGTTTTTGGTCAATTAGGGGTCCCTAAAGCTGACGGGAAACTTCCAAGACGTGTTGAGGGAAAGCTAATGAAGAGTTTTGTTGAAGAAATTGCTTGTGGTGCTAATCATGTTGCAGTTTTAACTTCAAGAACTGAGGTTTACACTTGGGGAAAGGGTGCAAATGGTCGATTAGGTCTTCGAGATACAGAAGATAAATTTTCACCAACATTAGTTGAAGCTTTAAAAGATAAACAAGTCAAGAGTATTGTCTGTGGCACTAACTTTACTGCTGCTATCTGTCTTCACAAATGGCTCTCTGGTATTGATCGATCAATGTGTTCTGGCTGTCGCCTTCCATTTAATTTTAAAAGAAAAAGACACAATTGTTATAATTGTGGACTTGTGTTATGCCATTCATGCAGCAGCAAAAAGTGTCTCAAGGCTTCCATGGCACCAAACCCAAACAAACCTTATCGGGTCTGTGATATTTGTTTTGCTAAACTTCGGAAGGCCACTGAAACCAACTCTTCATCTGGGTCTGCTATTAGTAGAAGAGGAAGTATGAATCAGGGATTCAACGAACTGATTCAAACCAATTTTAAAGAAATGGAAAGTGGATCTTCCAAAAGAAACAAGAAAATGGATTTCAGCAGCAATTGTGTTATTCCCATTGCAAATGGAGTTTCTGAGCGTCGAGCACTCAATGGGACTAAATCTTTCAACCCAGTTTTTGGATCATCCAAAAAGTTTTTCTCAGCTTCGCTTCCTGGATCCAGAATTGCATCTCGAGCAACATCACCAACATTGAGAAGTGATCGATCTAGTCCACCTCGTTCCACAACACCAACCCCAGCTCTTTCGGGGCTTACTCTTCCAAAAGTTGGGGATGATGCTAAAAGGAAAAATGTCAGTCTGAGTGAAGAAGTTCTAAAATTAAAAGCTCAGGTATGTTGCTTGTTGAACGATCTTACTCGTAAAGCTCAACTTCGAGAAATTGAGATGGAAAAAACAGCCCAACAGTTGAAGGAGGCTCTGAAAGTAGCAGGGGCAGAAACTGTGAAATGCAAAGCAGCAAAGGAAGTAATTAAGTCGCTCACAGCCCAATTAAAGGAAATGGCTGAAAGGTTACCAATTGGAACAGCAAGAAACATGGATACTCGTCCATTAGACTCTTCCAGTCCCACTCGTGAAGTTTCTACTACAGCCGTTGAGAAATCAAGCAGTACATTGAATTTCTCTGAACCTTATTCGATTGTATCGAGCAGTGAGATAATTTCTGACGTGCCACGGGCCACTTCGAATCACACTGAAGTGGCACATTCAGAAGCAACAGCTAGGCATAAAAACAGAGGAGCAAAAGCTGAAACAGCACAAGGGGATGAATGGGTTGAGCAAGATGAGCCTGGTGTATATATTACCCTAGTTTCCTTGCCTGGAGGTGTCAAAGATCTCAAGCGTGTTCGCTTCAGTCGAAAGCGGTTCAGTGAGAAACAAGCAGAGCAGTGGTGGGCGGCAAATAAGGGTAGAGTATATCAGCAGTACAATGTTCCGGTGGTTGAAAAATCCAGTATTCCGACGGGGAGGGAAGGGTTAGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N BRX_assoc FYVE PH_12 RCC1

Protein Analysis

1085

Amino Acids

117.24

Weight (kDa)

9.18

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 26 - 128 2.7e-07 Pleckstrin homology domain
WD40_RLD PF25390 266 - 649 4.2e-80 RCC1-like domain
RCC1_2 PF13540 310 - 333 2.5e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 326 - 375 1.1e-09 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 327 - 438 3.8e-06 Alsin RCC1-like domain
RCC1_2 PF13540 365 - 388 3.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 378 - 430 1.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 417 - 445 8.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 434 - 482 1.2e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 497 - 546 5.8e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 550 - 598 6.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 603 - 650 1.4e-13 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 655 - 720 1.7e-13 FYVE zinc finger
BRX_N PF13713 902 - 931 1.2e-13 Transcription factor BRX N-terminal domain
BRX PF08381 1011 - 1065 2.1e-28 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2043
AarI CACCTGC 1 cut(s) 698
Acc36I ACCTGC 1 cut(s) 698
AccB1I GGYRCC 1 cut(s) 2104
AccB7I CCANNNNNTGG 2 cut(s) 2384, 2534
AccI GTMKAC 1 cut(s) 1528
AciI CCGC 4 cut(s) 26, 41, 3130, 3164
AclWI GGATC 6 cut(s) 36, 853, 2278, 2395, 2418, 2431
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 5 cut(s) 131, 1088, 1242, 1868, 2884
AcuI CTGAAG 4 cut(s) 372, 621, 2982, 3103
AcvI CACGTG 1 cut(s) 389
AfaI GTAC 8 cut(s) 179, 231, 921, 1145, 1162, 1405, 2878, 3194
AfiI CCNNNNNNNGG 9 cut(s) 258, 572, 668, 1238, 1299, 1679, 2384, 2534, 2761
AflIII ACRYGT 2 cut(s) 1140, 1723
AjiI CACGTC 2 cut(s) 1724, 2935
AjnI CCWGG 5 cut(s) 232, 1373, 2419, 3055, 3085
AjuI GAANNNNNNNTTGG 2 cut(s) 2387, 2419
AleI CACNNNNGTG 1 cut(s) 2963
Alw21I GWGCWC 2 cut(s) 488, 2353
Alw26I GTCTC 4 cut(s) 403, 1255, 1403, 2093
AlwI GGATC 6 cut(s) 36, 853, 2278, 2395, 2418, 2431
AlwNI CAGNNNCTG 2 cut(s) 644, 2708
Ama87I CYCGRG 1 cut(s) 2439
AoxI GGCC 7 cut(s) 43, 69, 269, 391, 1648, 2163, 2943
ApaI GGGCCC 1 cut(s) 47
ApoI RAATTY 5 cut(s) 131, 1088, 1242, 1868, 2884
AseI ATTAAT 1 cut(s) 380
Asp700I GAANNNNTTC 4 cut(s) 165, 534, 2238, 2577
AspLEI GCGC 3 cut(s) 117, 243, 1177
AspS9I GGNCC 9 cut(s) 43, 44, 1210, 1229, 1268, 1351, 1648, 1693, 2943
AsuHPI GGTGA 6 cut(s) 515, 1010, 1148, 1553, 1866, 2442
AsuII TTCGAA 1 cut(s) 2951
AvaI CYCGRG 1 cut(s) 2439
AvaII GGWCC 5 cut(s) 1210, 1229, 1268, 1351, 1693
BaeGI GKGCMC 4 cut(s) 47, 97, 1374, 1566
BalI TGGCCA 1 cut(s) 71
BamHI GGATCC 1 cut(s) 2423
BanI GGYRCC 1 cut(s) 2104
BanII GRGCYC 2 cut(s) 47, 488
BarI GAAGNNNNNNTAC 2 cut(s) 415, 447
BauI CACGAG 1 cut(s) 2838
BbrPI CACGTG 1 cut(s) 389
BbsI GAAGAC 2 cut(s) 1841, 1946
Bbv12I GWGCWC 2 cut(s) 488, 2353
BbvI GCAGC 9 cut(s) 535, 631, 946, 1451, 1930, 2085, 2088, 2316, 2735
BccI CCATC 8 cut(s) 80, 515, 670, 674, 874, 1316, 1508, 2647
BceAI ACGGC 2 cut(s) 968, 2843
BciT130I CCWGG 5 cut(s) 234, 1375, 2421, 3057, 3087
BciVI GTATCC 2 cut(s) 273, 2800
BcoDI GTCTC 4 cut(s) 403, 1255, 1403, 2093
BfaI CTAG 5 cut(s) 870, 1340, 2475, 2988, 3074
BfmI CTRYAG 3 cut(s) 915, 1437, 2853
BfuAI ACCTGC 1 cut(s) 698
BfuI GTATCC 2 cut(s) 273, 2800
BglII AGATCT 1 cut(s) 3100
BmcAI AGTACT 2 cut(s) 921, 1162
Bme1390I CCNGG 5 cut(s) 234, 1375, 2421, 3057, 3087
Bme18I GGWCC 5 cut(s) 1210, 1229, 1268, 1351, 1693
BmeT110I CYCGRG 1 cut(s) 2439
BmgBI CACGTC 2 cut(s) 1724, 2935
BmgT120I GGNCC 9 cut(s) 43, 44, 1210, 1229, 1268, 1351, 1648, 1693, 2943
BmiI GGNNCC 9 cut(s) 45, 531, 1211, 1212, 1352, 1694, 1695, 2106, 2425
BmrFI CCNGG 5 cut(s) 234, 1375, 2421, 3057, 3087
BmrI ACTGGG 1 cut(s) 2372
BmsI GCATC 8 cut(s) 631, 706, 775, 1024, 1054, 1057, 2444, 2533
BmuI ACTGGG 1 cut(s) 2372
BpiI GAAGAC 2 cut(s) 1841, 1946
BplI GAGNNNNNCTC 2 cut(s) 250, 282
BpmI CTGGAG 1 cut(s) 3108
Bpu10I CCTNAGC 1 cut(s) 2595
Bpu14I TTCGAA 1 cut(s) 2951
BpuEI CTTGAG 3 cut(s) 89, 2075, 3089
Bsa29I ATCGAT 2 cut(s) 1981, 2470
BsaAI YACGTR 1 cut(s) 389
BsaI GGTCTC 2 cut(s) 403, 1403
BsaJI CCNNGG 7 cut(s) 266, 1180, 1271, 1373, 1374, 2100, 2939
BsaWI WCCGGW 2 cut(s) 1133, 3202
Bsc4I CCNNNNNNNGG 9 cut(s) 258, 572, 668, 1238, 1299, 1679, 2384, 2534, 2761
Bse1I ACTGG 4 cut(s) 370, 2378, 2829, 3219
Bse3DI GCAATG 1 cut(s) 2322
BseBI CCWGG 5 cut(s) 234, 1375, 2421, 3057, 3087
BseCI ATCGAT 2 cut(s) 1981, 2470
BseDI CCNNGG 7 cut(s) 266, 1180, 1271, 1373, 1374, 2100, 2939
BseLI CCNNNNNNNGG 9 cut(s) 258, 572, 668, 1238, 1299, 1679, 2384, 2534, 2761
BseMI GCAATG 1 cut(s) 2322
BseMII CTCAG 9 cut(s) 195, 429, 669, 690, 1797, 2331, 2421, 2558, 2609
BseNI ACTGG 4 cut(s) 370, 2378, 2829, 3219
BseRI GAGGAG 2 cut(s) 206, 3018
BseSI GKGCMC 4 cut(s) 47, 97, 1374, 1566
BseXI GCAGC 9 cut(s) 535, 631, 946, 1451, 1930, 2085, 2088, 2316, 2735
BseYI CCCAGC 2 cut(s) 1298, 2503
BsgI GTGCAG 1 cut(s) 726
BshFI GGCC 7 cut(s) 45, 71, 271, 393, 1650, 2165, 2945
BshNI GGYRCC 1 cut(s) 2104
BshVI ATCGAT 2 cut(s) 1981, 2470
BsiHKAI GWGCWC 2 cut(s) 488, 2353
BsiHKCI CYCGRG 1 cut(s) 2439
BsiSI CCGG 2 cut(s) 1134, 3203
BslFI GGGAC 6 cut(s) 71, 1196, 1364, 1679, 2374, 2817
BslI CCNNNNNNNGG 9 cut(s) 258, 572, 668, 1238, 1299, 1679, 2384, 2534, 2761
BsmAI GTCTC 4 cut(s) 403, 1255, 1403, 2093
BsmFI GGGAC 6 cut(s) 71, 1196, 1364, 1679, 2374, 2817
BsnI GGCC 7 cut(s) 45, 71, 271, 393, 1650, 2165, 2945
Bso31I GGTCTC 2 cut(s) 403, 1403
BsoBI CYCGRG 1 cut(s) 2439
Bsp119I TTCGAA 1 cut(s) 2951
Bsp120I GGGCCC 1 cut(s) 43
Bsp1286I GDGCHC 6 cut(s) 47, 97, 488, 1374, 1566, 2353
Bsp1407I TGTACA 1 cut(s) 1143
Bsp19I CCATGG 1 cut(s) 2100
BspACI CCGC 4 cut(s) 26, 41, 3130, 3164
BspANI GGCC 7 cut(s) 45, 71, 271, 393, 1650, 2165, 2945
BspCNI CTCAG 9 cut(s) 194, 430, 668, 689, 1798, 2332, 2420, 2559, 2608
BspDI ATCGAT 2 cut(s) 1981, 2470
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 9 cut(s) 45, 531, 1211, 1212, 1352, 1694, 1695, 2106, 2425
BspMAI CTGCAG 1 cut(s) 1441
BspMI ACCTGC 1 cut(s) 698
BspPI GGATC 6 cut(s) 36, 853, 2278, 2395, 2418, 2431
BspQI GCTCTTC 1 cut(s) 1492
BspT104I TTCGAA 1 cut(s) 2951
BspT107I GGYRCC 1 cut(s) 2104
BspTNI GGTCTC 2 cut(s) 403, 1403
BsrDI GCAATG 1 cut(s) 2322
BsrGI TGTACA 1 cut(s) 1143
BsrI ACTGG 4 cut(s) 370, 2378, 2829, 3219
BssECI CCNNGG 7 cut(s) 266, 1180, 1271, 1373, 1374, 2100, 2939
BssSI CACGAG 1 cut(s) 2838
BssT1I CCWWGG 2 cut(s) 1180, 2100
Bst2BI CACGAG 1 cut(s) 2838
Bst2UI CCWGG 5 cut(s) 234, 1375, 2421, 3057, 3087
Bst4CI ACNGT 7 cut(s) 449, 534, 1272, 1408, 1630, 2673, 2710
Bst6I CTCTTC 6 cut(s) 1492, 1739, 2185, 2200, 2529, 2830
BstAUI TGTACA 1 cut(s) 1143
BstBAI YACGTR 1 cut(s) 389
BstBI TTCGAA 1 cut(s) 2951
BstC8I GCNNGC 2 cut(s) 43, 957
BstDSI CCRYGG 3 cut(s) 1271, 2100, 2939
BstEII GGTNACC 1 cut(s) 2778
BstENI CCTNNNNNAGG 1 cut(s) 256
BstHHI GCGC 3 cut(s) 117, 243, 1177
BstMAI GTCTC 4 cut(s) 403, 1255, 1403, 2093
BstMWI GCNNNNNNNGC 9 cut(s) 32, 77, 650, 1772, 2070, 2102, 2441, 3020, 3052
BstNI CCWGG 5 cut(s) 234, 1375, 2421, 3057, 3087
BstNSI RCATGY 3 cut(s) 959, 1101, 1144
BstPI GGTNACC 1 cut(s) 2778
BstSCI CCNGG 5 cut(s) 232, 1373, 2419, 3055, 3085
BstSFI CTRYAG 3 cut(s) 915, 1437, 2853
BstSLI GKGCMC 4 cut(s) 47, 97, 1374, 1566
BstV1I GCAGC 9 cut(s) 535, 631, 946, 1451, 1930, 2085, 2088, 2316, 2735
BstV2I GAAGAC 2 cut(s) 1841, 1946
BstX2I RGATCY 3 cut(s) 2270, 2423, 3100
BstYI RGATCY 3 cut(s) 2270, 2423, 3100
Bsu15I ATCGAT 2 cut(s) 1981, 2470
BsuI GTATCC 2 cut(s) 273, 2800
BsuRI GGCC 7 cut(s) 45, 71, 271, 393, 1650, 2165, 2945
BsuTUI ATCGAT 2 cut(s) 1981, 2470
BtgI CCRYGG 3 cut(s) 1271, 2100, 2939
BtgZI GCGATG 1 cut(s) 1335
BtrI CACGTC 2 cut(s) 1724, 2935
BtsI GCAGTG 4 cut(s) 75, 1434, 2923, 3161
BtsIMutI CAGTG 8 cut(s) 75, 1374, 1434, 2166, 2923, 2958, 3142, 3161
BveI ACCTGC 1 cut(s) 698
Cac8I GCNNGC 2 cut(s) 43, 957
CaiI CAGNNNCTG 2 cut(s) 644, 2708
CciI TCATGA 1 cut(s) 772
CfoI GCGC 3 cut(s) 117, 243, 1177
Cfr13I GGNCC 9 cut(s) 43, 44, 1210, 1229, 1268, 1351, 1648, 1693, 2943
ClaI ATCGAT 2 cut(s) 1981, 2470
CseI GACGC 1 cut(s) 2333
CsiI ACCWGGT 1 cut(s) 232
Csp6I GTAC 8 cut(s) 178, 230, 920, 1144, 1161, 1404, 2877, 3193
CspCI CAANNNNNGTGG 2 cut(s) 1555, 1590
CviQI GTAC 8 cut(s) 178, 230, 920, 1144, 1161, 1404, 2877, 3193
DraI TTTAAA 3 cut(s) 1896, 2020, 2254
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 6 cut(s) 1492, 1739, 2185, 2200, 2529, 2830
EarI CTCTTC 6 cut(s) 1492, 1739, 2185, 2200, 2529, 2830
Ecl136II GAGCTC 1 cut(s) 486
Eco130I CCWWGG 2 cut(s) 1180, 2100
Eco24I GRGCYC 2 cut(s) 47, 488
Eco31I GGTCTC 2 cut(s) 403, 1403
Eco47I GGWCC 5 cut(s) 1210, 1229, 1268, 1351, 1693
Eco53kI GAGCTC 1 cut(s) 486
Eco57I CTGAAG 4 cut(s) 372, 621, 2982, 3103
Eco72I CACGTG 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 2439
Eco91I GGTNACC 1 cut(s) 2778
EcoICRI GAGCTC 1 cut(s) 486
EcoNI CCTNNNNNAGG 1 cut(s) 256
EcoO109I RGGNCCY 2 cut(s) 1210, 1693
EcoO65I GGTNACC 1 cut(s) 2778
EcoRI GAATTC 1 cut(s) 1242
EcoRII CCWGG 5 cut(s) 232, 1373, 2419, 3055, 3085
EcoT14I CCWWGG 2 cut(s) 1180, 2100
EcoT22I ATGCAT 1 cut(s) 721
EcoT38I GRGCYC 2 cut(s) 47, 488
ErhI CCWWGG 2 cut(s) 1180, 2100
FaqI GGGAC 6 cut(s) 71, 1196, 1364, 1679, 2374, 2817
FauI CCCGC 1 cut(s) 34
FblI GTMKAC 1 cut(s) 1528
FriOI GRGCYC 2 cut(s) 47, 488
FspBI CTAG 5 cut(s) 870, 1340, 2475, 2988, 3074
GlaI GCGC 3 cut(s) 116, 242, 1176
GsaI CCCAGC 2 cut(s) 1302, 2507
GsuI CTGGAG 1 cut(s) 3108
HaeIII GGCC 7 cut(s) 45, 71, 271, 393, 1650, 2165, 2945
HapII CCGG 2 cut(s) 1134, 3203
HgaI GACGC 1 cut(s) 2333
HhaI GCGC 3 cut(s) 117, 243, 1177
Hin6I GCGC 3 cut(s) 115, 241, 1175
HinP1I GCGC 3 cut(s) 115, 241, 1175
HincII GTYRAC 1 cut(s) 1529
HindII GTYRAC 1 cut(s) 1529
HindIII AAGCTT 2 cut(s) 1496, 1890
HpaII CCGG 2 cut(s) 1134, 3203
HphI GGTGA 6 cut(s) 515, 1010, 1148, 1553, 1866, 2442
Hpy99I CGWCG 2 cut(s) 2349, 3232
HpyCH4III ACNGT 7 cut(s) 449, 534, 1272, 1408, 1630, 2673, 2710
HpyCH4IV ACGT 3 cut(s) 388, 1723, 2934
HpyF10VI GCNNNNNNNGC 9 cut(s) 32, 77, 650, 1772, 2070, 2102, 2441, 3020, 3052
HpySE526I ACGT 3 cut(s) 388, 1723, 2934
HspAI GCGC 3 cut(s) 115, 241, 1175
KflI GGGWCCC 2 cut(s) 1210, 1693
LguI GCTCTTC 1 cut(s) 1492
LmnI GCTCC 2 cut(s) 1182, 3005
Lsp1109I GCAGC 9 cut(s) 535, 631, 946, 1451, 1930, 2085, 2088, 2316, 2735
LweI GCATC 8 cut(s) 631, 706, 775, 1024, 1054, 1057, 2444, 2533
MabI ACCWGGT 1 cut(s) 232
MaeI CTAG 5 cut(s) 870, 1340, 2475, 2988, 3074
MaeII ACGT 3 cut(s) 388, 1723, 2934
MaeIII GTNAC 6 cut(s) 967, 1123, 1136, 1288, 1541, 2778
MfeI CAATTG 6 cut(s) 98, 1304, 1480, 2035, 2308, 2784
MflI RGATCY 3 cut(s) 2270, 2423, 3100
MhlI GDGCHC 6 cut(s) 47, 97, 488, 1374, 1566, 2353
MlsI TGGCCA 1 cut(s) 71
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 1 cut(s) 2816
MmeI TCCRAC 1 cut(s) 3251
Mox20I TGGCCA 1 cut(s) 71
Mph1103I ATGCAT 1 cut(s) 721
MroXI GAANNNNTTC 4 cut(s) 165, 534, 2238, 2577
MscI TGGCCA 1 cut(s) 71
MslI CAYNNNNRTG 4 cut(s) 543, 1116, 1367, 2963
Msp20I TGGCCA 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 1283
MspI CCGG 2 cut(s) 1134, 3203
MspR9I CCNGG 5 cut(s) 234, 1375, 2421, 3057, 3087
MunI CAATTG 6 cut(s) 98, 1304, 1480, 2035, 2308, 2784
MvaI CCWGG 5 cut(s) 234, 1375, 2421, 3057, 3087
MwoI GCNNNNNNNGC 9 cut(s) 32, 77, 650, 1772, 2070, 2102, 2441, 3020, 3052
NcoI CCATGG 1 cut(s) 2100
NlaIV GGNNCC 9 cut(s) 45, 531, 1211, 1212, 1352, 1694, 1695, 2106, 2425
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 4 cut(s) 1123, 1136, 1288, 1541
NsiI ATGCAT 1 cut(s) 721
NspI RCATGY 3 cut(s) 959, 1101, 1144
NspV TTCGAA 1 cut(s) 2951
OliI CACNNNNGTG 1 cut(s) 2963
PaeI GCATGC 1 cut(s) 959
PaeR7I CTCGAG 1 cut(s) 2439
PagI TCATGA 1 cut(s) 772
PaqCI CACCTGC 1 cut(s) 698
PasI CCCWGGG 1 cut(s) 1374
PciI ACATGT 1 cut(s) 1140
PciSI GCTCTTC 1 cut(s) 1492
PcsI WCGNNNNNNNCGW 2 cut(s) 2909, 3121
PdmI GAANNNNTTC 4 cut(s) 165, 534, 2238, 2577
PfeI GAWTC 9 cut(s) 161, 416, 884, 908, 1385, 2218, 2226, 2239, 2953
PflMI CCANNNNNTGG 2 cut(s) 2384, 2534
PfoI TCCNGGA 1 cut(s) 2419
PleI GAGTC 1 cut(s) 2816
PmaCI CACGTG 1 cut(s) 389
PmlI CACGTG 1 cut(s) 389
PpsI GAGTC 1 cut(s) 2816
Ppu21I YACGTR 1 cut(s) 389
PpuMI RGGWCCY 2 cut(s) 1210, 1693
PscI ACATGT 1 cut(s) 1140
PshBI ATTAAT 1 cut(s) 380
PsiI TTATAA 1 cut(s) 2043
Psp124BI GAGCTC 1 cut(s) 488
Psp5II RGGWCCY 2 cut(s) 1210, 1693
Psp6I CCWGG 5 cut(s) 232, 1373, 2419, 3055, 3085
PspCI CACGTG 1 cut(s) 389
PspEI GGTNACC 1 cut(s) 2778
PspFI CCCAGC 2 cut(s) 1298, 2503
PspGI CCWGG 5 cut(s) 232, 1373, 2419, 3055, 3085
PspN4I GGNNCC 9 cut(s) 45, 531, 1211, 1212, 1352, 1694, 1695, 2106, 2425
PspOMI GGGCCC 1 cut(s) 43
PspPI GGNCC 9 cut(s) 43, 44, 1210, 1229, 1268, 1351, 1648, 1693, 2943
PspPPI RGGWCCY 2 cut(s) 1210, 1693
PsrI GAACNNNNNNTAC 2 cut(s) 912, 944
PstI CTGCAG 1 cut(s) 1441
PstNI CAGNNNCTG 2 cut(s) 644, 2708
PsuI RGATCY 3 cut(s) 2270, 2423, 3100
PvuII CAGCTG 1 cut(s) 1283
RsaI GTAC 8 cut(s) 179, 231, 921, 1145, 1162, 1405, 2878, 3194
RsaNI GTAC 8 cut(s) 178, 230, 920, 1144, 1161, 1404, 2877, 3193
RseI CAYNNNNRTG 4 cut(s) 543, 1116, 1367, 2963
SacI GAGCTC 1 cut(s) 488
SalI GTCGAC 1 cut(s) 1527
SapI GCTCTTC 1 cut(s) 1492
Sau96I GGNCC 9 cut(s) 43, 44, 1210, 1229, 1268, 1351, 1648, 1693, 2943
ScaI AGTACT 2 cut(s) 921, 1162
SchI GAGTC 1 cut(s) 2816
ScrFI CCNGG 5 cut(s) 234, 1375, 2421, 3057, 3087
SduI GDGCHC 6 cut(s) 47, 97, 488, 1374, 1566, 2353
SexAI ACCWGGT 1 cut(s) 232
SfaNI GCATC 8 cut(s) 631, 706, 775, 1024, 1054, 1057, 2444, 2533
SfcI CTRYAG 3 cut(s) 915, 1437, 2853
Sfr274I CTCGAG 1 cut(s) 2439
SfuI TTCGAA 1 cut(s) 2951
SinI GGWCC 5 cut(s) 1210, 1229, 1268, 1351, 1693
SlaI CTCGAG 1 cut(s) 2439
SmiMI CAYNNNNRTG 4 cut(s) 543, 1116, 1367, 2963
SmlI CTYRAG 4 cut(s) 104, 2090, 2439, 3104
SmoI CTYRAG 4 cut(s) 104, 2090, 2439, 3104
SphI GCATGC 1 cut(s) 959
SsiI CCGC 4 cut(s) 26, 41, 3130, 3164
SspI AATATT 1 cut(s) 1084
SspMI CTAG 5 cut(s) 870, 1340, 2475, 2988, 3074
SstI GAGCTC 1 cut(s) 488
StyD4I CCNGG 5 cut(s) 232, 1373, 2419, 3055, 3085
StyI CCWWGG 2 cut(s) 1180, 2100
TaaI ACNGT 7 cut(s) 449, 534, 1272, 1408, 1630, 2673, 2710
TaiI ACGT 3 cut(s) 391, 1726, 2937
TaqII GACCGA 1 cut(s) 1368
TatI WGTACW 5 cut(s) 919, 1143, 1160, 2876, 3192
TauI GCSGC 1 cut(s) 3167
TfiI GAWTC 9 cut(s) 161, 416, 884, 908, 1385, 2218, 2226, 2239, 2953
TscAI CASTG 8 cut(s) 82, 1374, 1441, 2173, 2923, 2965, 3142, 3161
TseFI GTSAC 4 cut(s) 1123, 1136, 1288, 1541
Tsp45I GTSAC 4 cut(s) 1123, 1136, 1288, 1541
TspGWI ACGGA 1 cut(s) 1391
TspRI CASTG 8 cut(s) 82, 1374, 1441, 2173, 2923, 2965, 3142, 3161
Van91I CCANNNNNTGG 2 cut(s) 2384, 2534
VpaK11BI GGWCC 5 cut(s) 1210, 1229, 1268, 1351, 1693
VspI ATTAAT 1 cut(s) 380
XagI CCTNNNNNAGG 1 cut(s) 256
XapI RAATTY 5 cut(s) 131, 1088, 1242, 1868, 2884
XceI RCATGY 3 cut(s) 959, 1101, 1144
XhoI CTCGAG 1 cut(s) 2439
XmiI GTMKAC 1 cut(s) 1528
XmnI GAANNNNTTC 4 cut(s) 165, 534, 2238, 2577
XspI CTAG 5 cut(s) 870, 1340, 2475, 2988, 3074
ZrmI AGTACT 2 cut(s) 921, 1162
Zsp2I ATGCAT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.