FvH4_7g02960

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003738
Physical Location & Seq
Reverse (-)
3277178 .. 3288071
10894 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02960.t3

Sequence Viewer

Length: 3177 bp
ATGTTGCGCATGGAGAGGATGACTGCCGATCAGGCCAATCCGGGCCCGCTCGAGCGCGACATTCACTTGGCCATCACTGCTCTAAAAAAGGGGTCACAGTTACTGAAGTATGGACGCAGAGGAAAGCCCAAATTTTGTCCTTTCAGACTGTCCAATGATGAATCGGTTCTGATATGGTACTCAGGCAAAGATGAGAAATATGTTAAACTAAGCCATGTTTCTAGAATTATACCTGGCCAGCGCACTCCAATCTTTCAGAGGTATCCTCGGCCGGAGAAGGAATATCAGTCATTTTCTCTTATATACGATGACAGGTCTTTAGATTTGAAGGGGAGAGCAGAAACAAAAAGTGGAGTTACCTCTGAAGCAAATAGCCCAAGATCACATACGCAAAGAAGTTCTCCTTTGAGCTCTCCATTTTGTAGTGGTGATAGTTCACAGAAGGATGAAGTGGAACCCTTTCATCCTCATGCTACATCTGAAAGCCCTCCTAAAGTCCATTTGGAGAAGGCTTTATCAGATGCCATGCTATATGCCCTCCCTCCGAAAGTTCCCTTTCCTTCAGATTCTGCTTCTGGCTCATTTCAGTCTCTGTCATCAGGAGGCTCAGATGGAATGAATGGTCGAACAAAGGGTGCGGGGACAGACGCTTATAGAGTTAGTTTATCAAGTGCAGTTAGCTCATCAAGTCAAGGCTCTGGTCATGATGACGGTGACGCTTTAGGGGATGTTTTCATTTGGGGGGAAGGCGTAGGTGATTGCATTTTGGGTGGGGGATTACATAGAGTTGGTAGTTCTTCTAGTTCTAAGACGGACTCTTCTGTACCTAAAGTCTTGGAATCTGCTGTTGTACTTGATGTTCAAAGTATAGCTTGTGGTGGAAGACATGCTGCTTTTGTAACCAAGCAAGGAGAGGTATTTTCTTGGGGTGAAGAATTAGGAGGCAGACTTGGACATGGTGTAGATGCTGATATCTCCCATCCAAAGCTCATAGATGGTCTCAAAAATCTTAACGTAGAATTTTTAGCATGTGGGGAGTACCATTCTTGTGCAGTAACACTCTCTGGTGATATATACACATGGGGTGGCAGTACTTCTAATTTTGCACTCCTTGGACACGGGTTTCAATCCAGTCAGTGGATCCCAAAAAAGTTAAATGGACCTTTAGAGGGAATTCATGTCTCTTCAGTTTCTTGTGGACCATGGCACACAGCTGTTGTGACCTCTGCTGGTCAATTGTTTACTTTTGGTGATGGAACTTTTGGTGTTCTAGGTCATGGGGATCGCAAGTGTATATCAGTTCCAAGGGAAGTTGAGTCCCTTAAAGGCCTCCGTACTGTTCGAGTAGCTTGTGGTGTTTGGCACTCTGCTGCAGTAGTTGAAGTCATGGTGGGATCATCAAGTTCCAGCAACTGTTCTTCTGGGAAGCTTTTTACATGGGGAGATGGTGATAAAGGTCGACTTGGGCATGGTGATAAGGATGCAAGACTTGTGCCCACTTGTGTTGCTGCTTTGGTTGAACCCAACTTCTGTCAAGTTGCATGTGGACAAAGTCTGACTGTTGCCCTTACAAGAACTGGCCATGTTTACACTGTAGGAAGCCCTGTTTATGGTCAATTAGGGATCCCTCAAGCTGATGGGAAACTCCCGCGCCGTGTTGAGGGAAAGCTAATGAAGAATTTTGTTGAAGAAATTGCTTGTGGTGCTTATCATGTTGCAGTCTTAACGTCGAGAACTGAAGTTTACACTTGGGGAAAGGGTGCAAATGGTAGGTTAGGTCTTGGGGATACAGAGGATAAATTTTCACCAACATTAGTTGAAGCACTAAGAGACAAACAAGTCAAAAGTATTGTTTGCGGCACTAACTTTACAGCAGCCATCTGTCTTCATAAATGGGTCTCTGGTGTTGATCAATCAATGTGTTCTGGCTGTCGCCTTCCATTTAACTTTAAAAGAAAAAGACACAACTGCTACAATTGTGGACTTGTATTATGCCATTCGTGCAGTAGCAAAAAGTGCCTCAAGGCTGCGATGGCACCAAATCCGAACAAACCATATCGAGTTTGTGATAATTGTTTTGGGAAACTTAGAAAGGCCTCTGAAACCAACTATTCATCTGGTTCTGCTTTGAGAAAAGGAAGTGTAAATCAGGGATTCGCTGAAGTGAGTGAAAATAATAAAGATTTGGACAGTGGTTCAACCACAAGAAGCAAGAAACTACAGTTTAGCAGTAATTGTGTTGCTCCTTTTCCACATGACATCTCTGAGCGCCGTGCATTCAGTGGGACTAAATCTTTCAACCCAGTATTTGGATCTTCCAAAAAGTTTTTCTCAGCTTCTCTTCCTGGATCAAGAATTGCTTCTCGAGCAACATCACCAACATCCAGGCGATCTAGTCCGCCTCGTGCGAAAACACCAACCCCTGCTGCTCTCTCAGGGATCACGTTGCCAAATGTTGTGGAATATGCTAGAGGGACAAATGATGGTCTAAGTGAAGAAGTTCTTAAATTAAGAGCTCAGGTTGAAGATCTTACTCGTAAAAGTCGAGTTCAAGATATTGAGCTGGAAAGAACAACGCACCAATTAAAGGAGGCTGTCAAAATAGCAGGGGAAGAAAGTGCAAAATGTAGAGCAGCAAAGGAAGTAATTAAAGCGCTCACAGCTCAATTAAAAGAAATGGCTGAAAGGTTACCCATTAGAACAACAAGAATCAGTGACATACGTCCATTAGGCTCTTCTAGTCCTACCCATGAAGTTTCTACTGCAGCCACTGAGAAAGCAAGCAGTCCGTTGACCTCCCTTGAACCACATTCTATTGCATCAAATACCCTGGTGGTTTCTAATGCACCAAGGGTCTTGAGTAATCATGCTTCAAATACCACTGAAGTGGCAAATTCAGAAGCAACTGCTAGGCATAAGAACAGGGGAACAAAAGCTGATACCACACATGGGGATGAATGGGTTGAGCAAGACGAGCCTGGTGTATATATTACCCTTGTTTCCTCACCAACAGGAGTCAAAGATCTCAAGCGTGTTCGCTTCAGTCGAAAGCGGTTCAGTGAGAAACAAGCAGAACAGTGGTGGGCGGCAAATAGGGCTAGAGTATATCAGCAGTATAATGTTCCTGTGGTTGAAAAATCTAGTATTTCCGTAGGAAGAGAAGGATTGGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N BRX_assoc FYVE PH_12 RCC1

Protein Analysis

1059

Amino Acids

114.02

Weight (kDa)

9.15

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 26 - 128 9.6e-08 Pleckstrin homology domain
WD40_RLD PF25390 268 - 651 2.6e-81 RCC1-like domain
RCC1_2 PF13540 312 - 335 2.1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 328 - 377 7.1e-10 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 328 - 440 4.6e-06 Alsin RCC1-like domain
RCC1_2 PF13540 367 - 390 3.4e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 380 - 432 1.1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 419 - 447 6.9e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 436 - 484 7.2e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 499 - 548 7.5e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 552 - 600 3.1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 605 - 652 1.3e-14 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 656 - 722 1.7e-14 FYVE zinc finger
BRX_N PF13713 896 - 914 4.4e-06 Transcription factor BRX N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1838
Acc16I TGCGCA 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 2035
AccB7I CCANNNNNTGG 2 cut(s) 1137, 2309
AccBSI CCGCTC 1 cut(s) 49
AccI GTMKAC 1 cut(s) 1459
AccII CGCG 2 cut(s) 57, 1651
AciI CCGC 7 cut(s) 47, 638, 1649, 1857, 2399, 3052, 3086
AclWI GGATC 9 cut(s) 1135, 1148, 1290, 1402, 1618, 1631, 2320, 2356, 2447
AcoI YGGCCR 4 cut(s) 69, 235, 269, 1579
AcsI RAATTY 6 cut(s) 131, 1019, 1173, 1678, 1799, 2893
AcuI CTGAAG 8 cut(s) 125, 384, 546, 1170, 1758, 2181, 2904, 3025
AfaI GTAC 6 cut(s) 179, 825, 852, 1040, 1093, 1336
AfeI AGCGCT 1 cut(s) 2655
AfiI CCNNNNNNNGG 7 cut(s) 1137, 1169, 1610, 1660, 2309, 2587, 2949
AjnI CCWGG 5 cut(s) 232, 2344, 2384, 2829, 2977
AjuI GAANNNNNNNTTGG 2 cut(s) 2312, 2344
AleI CACNNNNGTG 1 cut(s) 2885
Alw21I GWGCWC 2 cut(s) 413, 2518
Alw26I GTCTC 5 cut(s) 594, 1004, 1186, 1824, 1903
AlwI GGATC 9 cut(s) 1135, 1148, 1290, 1402, 1618, 1631, 2320, 2356, 2447
AlwNI CAGNNNCTG 5 cut(s) 103, 569, 592, 1413, 2771
Ama87I CYCGRG 2 cut(s) 50, 2364
Aor51HI AGCGCT 1 cut(s) 2655
AoxI GGCC 8 cut(s) 33, 43, 69, 235, 269, 1327, 1579, 2094
ApaI GGGCCC 1 cut(s) 47
ApeKI GCWGC 8 cut(s) 890, 1370, 1508, 1874, 2027, 2426, 2633, 2765
ApoI RAATTY 6 cut(s) 131, 1019, 1173, 1678, 1799, 2893
Asp700I GAANNNNTTC 4 cut(s) 165, 459, 2359, 2499
AspLEI GCGC 6 cut(s) 9, 57, 243, 1653, 2271, 2656
AspS9I GGNCC 4 cut(s) 43, 44, 1160, 1199
AsuC2I CCSGG 1 cut(s) 42
AvaI CYCGRG 2 cut(s) 50, 2364
AvaII GGWCC 2 cut(s) 1160, 1199
BaeGI GKGCMC 2 cut(s) 47, 1497
BalI TGGCCA 3 cut(s) 71, 237, 1581
BamHI GGATCC 2 cut(s) 1140, 1623
BanI GGYRCC 1 cut(s) 2035
BanII GRGCYC 3 cut(s) 47, 413, 2518
BarI GAAGNNNNNNTAC 2 cut(s) 2854, 2886
BauI CACGAG 1 cut(s) 2403
BbsI GAAGAC 2 cut(s) 889, 1877
Bbv12I GWGCWC 2 cut(s) 413, 2518
BbvI GCAGC 8 cut(s) 877, 1357, 1495, 1886, 2014, 2413, 2645, 2777
BceAI ACGGC 2 cut(s) 1638, 2256
BciT130I CCWGG 5 cut(s) 234, 2346, 2386, 2831, 2979
BciVI GTATCC 2 cut(s) 273, 1780
BclI TGATCA 1 cut(s) 1909
BcnI CCSGG 1 cut(s) 42
BcoDI GTCTC 5 cut(s) 594, 1004, 1186, 1824, 1903
BfaI CTAG 9 cut(s) 222, 801, 1271, 2394, 2469, 2739, 2910, 3099, 3141
BfmI CTRYAG 4 cut(s) 1371, 1593, 2219, 2763
BfoI RGCGCY 2 cut(s) 2272, 2657
BfuI GTATCC 2 cut(s) 273, 1780
BglI GCCNNNNNGGC 1 cut(s) 32
BglII AGATCT 2 cut(s) 2527, 3022
BmcAI AGTACT 1 cut(s) 1093
Bme1390I CCNGG 6 cut(s) 42, 234, 2346, 2386, 2831, 2979
Bme18I GGWCC 2 cut(s) 1160, 1199
BmeT110I CYCGRG 2 cut(s) 50, 2364
BmgT120I GGNCC 4 cut(s) 43, 44, 1160, 1199
BmiI GGNNCC 5 cut(s) 45, 456, 1142, 1625, 2037
BmrFI CCNGG 6 cut(s) 42, 234, 2346, 2386, 2831, 2979
BmrI ACTGGG 1 cut(s) 2297
BmsI GCATC 4 cut(s) 511, 955, 1471, 2828
BmuI ACTGGG 1 cut(s) 2297
BoxI GACNNNNGTC 1 cut(s) 2721
BpiI GAAGAC 2 cut(s) 889, 1877
BplI GAGNNNNNCTC 2 cut(s) 250, 282
Bpu10I CCTNAGC 1 cut(s) 2517
BpuEI CTTGAG 4 cut(s) 1614, 2006, 2878, 3011
BpuMI CCSGG 1 cut(s) 42
BsaI GGTCTC 2 cut(s) 1004, 1903
BsaJI CCNNGG 6 cut(s) 266, 1111, 1202, 1304, 2829, 2849
Bsc4I CCNNNNNNNGG 7 cut(s) 1137, 1169, 1610, 1660, 2309, 2587, 2949
Bse1I ACTGG 3 cut(s) 1131, 1582, 2303
BseBI CCWGG 5 cut(s) 234, 2346, 2386, 2831, 2979
BseDI CCNNGG 6 cut(s) 266, 1111, 1202, 1304, 2829, 2849
BseGI GGATG 8 cut(s) 24, 451, 463, 733, 979, 1486, 2381, 2959
BseLI CCNNNNNNNGG 7 cut(s) 1137, 1169, 1610, 1660, 2309, 2587, 2949
BseMII CTCAG 7 cut(s) 195, 621, 2256, 2346, 2448, 2531, 2763
BseNI ACTGG 3 cut(s) 1131, 1582, 2303
BseSI GKGCMC 2 cut(s) 47, 1497
BseX3I CGGCCG 1 cut(s) 269
BseXI GCAGC 8 cut(s) 877, 1357, 1495, 1886, 2014, 2413, 2645, 2777
BsgI GTGCAG 3 cut(s) 693, 1071, 2023
Bsh1236I CGCG 2 cut(s) 57, 1651
Bsh1285I CGRYCG 1 cut(s) 272
BshFI GGCC 8 cut(s) 35, 45, 71, 237, 271, 1329, 1581, 2096
BshNI GGYRCC 1 cut(s) 2035
BsiEI CGRYCG 1 cut(s) 272
BsiHKAI GWGCWC 2 cut(s) 413, 2518
BsiHKCI CYCGRG 2 cut(s) 50, 2364
BsiSI CCGG 2 cut(s) 41, 272
BslFI GGGAC 4 cut(s) 655, 1303, 2299, 2488
BslI CCNNNNNNNGG 7 cut(s) 1137, 1169, 1610, 1660, 2309, 2587, 2949
BsmAI GTCTC 5 cut(s) 594, 1004, 1186, 1824, 1903
BsmFI GGGAC 4 cut(s) 655, 1303, 2299, 2488
BsmI GAATGC 1 cut(s) 2276
BsnI GGCC 8 cut(s) 35, 45, 71, 237, 271, 1329, 1581, 2096
Bso31I GGTCTC 2 cut(s) 1004, 1903
BsoBI CYCGRG 2 cut(s) 50, 2364
Bsp120I GGGCCC 1 cut(s) 43
Bsp1286I GDGCHC 4 cut(s) 47, 413, 1497, 2518
Bsp19I CCATGG 1 cut(s) 1202
BspACI CCGC 7 cut(s) 47, 638, 1649, 1857, 2399, 3052, 3086
BspANI GGCC 8 cut(s) 35, 45, 71, 237, 271, 1329, 1581, 2096
BspCNI CTCAG 7 cut(s) 194, 620, 2257, 2345, 2447, 2530, 2764
BspFNI CGCG 2 cut(s) 57, 1651
BspHI TCATGA 1 cut(s) 703
BspLI GGNNCC 5 cut(s) 45, 456, 1142, 1625, 2037
BspMAI CTGCAG 2 cut(s) 1375, 2767
BspPI GGATC 9 cut(s) 1135, 1148, 1290, 1402, 1618, 1631, 2320, 2356, 2447
BspQI GCTCTTC 1 cut(s) 2740
BspT107I GGYRCC 1 cut(s) 2035
BspTNI GGTCTC 2 cut(s) 1004, 1903
BsrBI CCGCTC 1 cut(s) 49
BsrI ACTGG 3 cut(s) 1131, 1582, 2303
BssECI CCNNGG 6 cut(s) 266, 1111, 1202, 1304, 2829, 2849
BssSI CACGAG 1 cut(s) 2403
BssT1I CCWWGG 4 cut(s) 1111, 1202, 1304, 2849
Bst2BI CACGAG 1 cut(s) 2403
Bst2UI CCWGG 5 cut(s) 234, 2346, 2386, 2831, 2979
Bst6I CTCTTC 5 cut(s) 823, 1189, 2346, 2740, 3151
BstAPI GCANNNNNTGC 1 cut(s) 2016
BstC8I GCNNGC 3 cut(s) 47, 239, 2782
BstDSI CCRYGG 1 cut(s) 1202
BstEII GGTNACC 1 cut(s) 2688
BstF5I GGATG 8 cut(s) 24, 451, 463, 733, 979, 1486, 2381, 2959
BstFNI CGCG 2 cut(s) 57, 1651
BstH2I RGCGCY 2 cut(s) 2272, 2657
BstHHI GCGC 6 cut(s) 9, 57, 243, 1653, 2271, 2656
BstMAI GTCTC 5 cut(s) 594, 1004, 1186, 1824, 1903
BstMCI CGRYCG 1 cut(s) 272
BstNI CCWGG 5 cut(s) 234, 2346, 2386, 2831, 2979
BstNSI RCATGY 3 cut(s) 890, 1032, 1545
BstPAI GACNNNNGTC 1 cut(s) 2721
BstPI GGTNACC 1 cut(s) 2688
BstSCI CCNGG 6 cut(s) 40, 232, 2344, 2384, 2829, 2977
BstSFI CTRYAG 4 cut(s) 1371, 1593, 2219, 2763
BstSLI GKGCMC 2 cut(s) 47, 1497
BstUI CGCG 2 cut(s) 57, 1651
BstV1I GCAGC 8 cut(s) 877, 1357, 1495, 1886, 2014, 2413, 2645, 2777
BstV2I GAAGAC 2 cut(s) 889, 1877
BstX2I RGATCY 5 cut(s) 1140, 1623, 2312, 2527, 3022
BstXI CCANNNNNNTGG 1 cut(s) 2887
BstYI RGATCY 5 cut(s) 1140, 1623, 2312, 2527, 3022
BstZI CGGCCG 1 cut(s) 269
BsuI GTATCC 2 cut(s) 273, 1780
BsuRI GGCC 8 cut(s) 35, 45, 71, 237, 271, 1329, 1581, 2096
BtgI CCRYGG 1 cut(s) 1202
BtgZI GCGATG 1 cut(s) 2045
BtsCI GGATG 8 cut(s) 24, 451, 463, 733, 979, 1486, 2381, 2959
BtsI GCAGTG 1 cut(s) 75
Cac8I GCNNGC 3 cut(s) 47, 239, 2782
CaiI CAGNNNCTG 5 cut(s) 103, 569, 592, 1413, 2771
CciI TCATGA 1 cut(s) 703
CfoI GCGC 6 cut(s) 9, 57, 243, 1653, 2271, 2656
Cfr13I GGNCC 4 cut(s) 43, 44, 1160, 1199
CseI GACGC 3 cut(s) 123, 656, 725
Csp6I GTAC 6 cut(s) 178, 824, 851, 1039, 1092, 1335
CspCI CAANNNNNGTGG 4 cut(s) 1486, 1521, 2439, 2474
CviQI GTAC 6 cut(s) 178, 824, 851, 1039, 1092, 1335
DraI TTTAAA 1 cut(s) 1951
DrdI GACNNNNNNGTC 1 cut(s) 1838
DseDI GACNNNNNNGTC 1 cut(s) 1838
EaeI YGGCCR 4 cut(s) 69, 235, 269, 1579
EagI CGGCCG 1 cut(s) 269
Eam1104I CTCTTC 5 cut(s) 823, 1189, 2346, 2740, 3151
EarI CTCTTC 5 cut(s) 823, 1189, 2346, 2740, 3151
EciI GGCGGA 1 cut(s) 2388
Ecl136II GAGCTC 2 cut(s) 411, 2516
EclXI CGGCCG 1 cut(s) 269
Eco130I CCWWGG 4 cut(s) 1111, 1202, 1304, 2849
Eco147I AGGCCT 2 cut(s) 1329, 2096
Eco24I GRGCYC 3 cut(s) 47, 413, 2518
Eco31I GGTCTC 2 cut(s) 1004, 1903
Eco32I GATATC 1 cut(s) 973
Eco47I GGWCC 2 cut(s) 1160, 1199
Eco47III AGCGCT 1 cut(s) 2655
Eco52I CGGCCG 1 cut(s) 269
Eco53kI GAGCTC 2 cut(s) 411, 2516
Eco57I CTGAAG 8 cut(s) 125, 384, 546, 1170, 1758, 2181, 2904, 3025
Eco88I CYCGRG 2 cut(s) 50, 2364
Eco91I GGTNACC 1 cut(s) 2688
EcoICRI GAGCTC 2 cut(s) 411, 2516
EcoO65I GGTNACC 1 cut(s) 2688
EcoRI GAATTC 1 cut(s) 1173
EcoRII CCWGG 5 cut(s) 232, 2344, 2384, 2829, 2977
EcoRV GATATC 1 cut(s) 973
EcoT14I CCWWGG 4 cut(s) 1111, 1202, 1304, 2849
EcoT38I GRGCYC 3 cut(s) 47, 413, 2518
ErhI CCWWGG 4 cut(s) 1111, 1202, 1304, 2849
FaqI GGGAC 4 cut(s) 655, 1303, 2299, 2488
FauI CCCGC 3 cut(s) 54, 631, 1656
FbaI TGATCA 1 cut(s) 1909
FblI GTMKAC 1 cut(s) 1459
FokI GGATG 8 cut(s) 31, 450, 458, 740, 966, 1493, 2368, 2966
FriOI GRGCYC 3 cut(s) 47, 413, 2518
FspBI CTAG 9 cut(s) 222, 801, 1271, 2394, 2469, 2739, 2910, 3099, 3141
FspI TGCGCA 1 cut(s) 8
GlaI GCGC 6 cut(s) 8, 56, 242, 1652, 2270, 2655
HaeII RGCGCY 2 cut(s) 2272, 2657
HaeIII GGCC 8 cut(s) 35, 45, 71, 237, 271, 1329, 1581, 2096
HapII CCGG 2 cut(s) 41, 272
HgaI GACGC 3 cut(s) 123, 656, 725
HhaI GCGC 6 cut(s) 9, 57, 243, 1653, 2271, 2656
Hin6I GCGC 6 cut(s) 7, 55, 241, 1651, 2269, 2654
HinP1I GCGC 6 cut(s) 7, 55, 241, 1651, 2269, 2654
HincII GTYRAC 2 cut(s) 1460, 2793
HindII GTYRAC 2 cut(s) 1460, 2793
HindIII AAGCTT 1 cut(s) 1427
HinfI GANTC 8 cut(s) 161, 566, 815, 839, 1316, 2154, 2709, 3015
HpaII CCGG 2 cut(s) 41, 272
Hpy166II GTNNAC 9 cut(s) 437, 1199, 1242, 1460, 1547, 1588, 1744, 1982, 2793
Hpy188III TCNNGA 8 cut(s) 222, 600, 704, 1731, 2352, 2364, 2552, 2857
Hpy8I GTNNAC 9 cut(s) 437, 1199, 1242, 1460, 1547, 1588, 1744, 1982, 2793
Hpy99I CGWCG 1 cut(s) 1732
HpyAV CCTTC 8 cut(s) 271, 322, 436, 502, 570, 740, 1946, 3155
HpyCH4IV ACGT 4 cut(s) 1014, 1727, 2444, 2722
HpySE526I ACGT 4 cut(s) 1014, 1727, 2444, 2722
HspAI GCGC 6 cut(s) 7, 55, 241, 1651, 2269, 2654
Ksp22I TGATCA 1 cut(s) 1909
LguI GCTCTTC 1 cut(s) 2740
LmnI GCTCC 1 cut(s) 2248
Lsp1109I GCAGC 8 cut(s) 877, 1357, 1495, 1886, 2014, 2413, 2645, 2777
LweI GCATC 4 cut(s) 511, 955, 1471, 2828
MaeI CTAG 9 cut(s) 222, 801, 1271, 2394, 2469, 2739, 2910, 3099, 3141
MaeII ACGT 4 cut(s) 1014, 1727, 2444, 2722
MaeIII GTNAC 9 cut(s) 93, 99, 355, 713, 898, 1054, 1219, 2688, 2714
MbiI CCGCTC 1 cut(s) 49
MfeI CAATTG 2 cut(s) 1235, 1975
MflI RGATCY 5 cut(s) 1140, 1623, 2312, 2527, 3022
MhlI GDGCHC 4 cut(s) 47, 413, 1497, 2518
MlsI TGGCCA 3 cut(s) 71, 237, 1581
MluNI TGGCCA 3 cut(s) 71, 237, 1581
MlyI GAGTC 3 cut(s) 809, 1325, 3024
Mox20I TGGCCA 3 cut(s) 71, 237, 1581
MroXI GAANNNNTTC 4 cut(s) 165, 459, 2359, 2499
MscI TGGCCA 3 cut(s) 71, 237, 1581
MslI CAYNNNNRTG 4 cut(s) 468, 1047, 2885, 2952
Msp20I TGGCCA 3 cut(s) 71, 237, 1581
MspA1I CMGCKG 1 cut(s) 1214
MspI CCGG 2 cut(s) 41, 272
MspR9I CCNGG 6 cut(s) 42, 234, 2346, 2386, 2831, 2979
MunI CAATTG 2 cut(s) 1235, 1975
Mva1269I GAATGC 1 cut(s) 2276
MvaI CCWGG 5 cut(s) 234, 2346, 2386, 2831, 2979
MvnI CGCG 2 cut(s) 57, 1651
NciI CCSGG 1 cut(s) 42
NcoI CCATGG 1 cut(s) 1202
NlaIV GGNNCC 5 cut(s) 45, 456, 1142, 1625, 2037
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 4 cut(s) 93, 713, 1219, 2714
NsbI TGCGCA 1 cut(s) 8
NspI RCATGY 3 cut(s) 890, 1032, 1545
OliI CACNNNNGTG 1 cut(s) 2885
PaeR7I CTCGAG 2 cut(s) 50, 2364
PagI TCATGA 1 cut(s) 703
PceI AGGCCT 2 cut(s) 1329, 2096
PciSI GCTCTTC 1 cut(s) 2740
PcsI WCGNNNNNNNCGW 2 cut(s) 2542, 3043
PctI GAATGC 1 cut(s) 2276
PdmI GAANNNNTTC 4 cut(s) 165, 459, 2359, 2499
PfeI GAWTC 5 cut(s) 161, 566, 839, 2154, 2709
PflFI GACNNNGTC 1 cut(s) 1551
PflMI CCANNNNNTGG 2 cut(s) 1137, 2309
PfoI TCCNGGA 1 cut(s) 2344
PleI GAGTC 3 cut(s) 809, 1324, 3023
PpsI GAGTC 3 cut(s) 809, 1324, 3023
PshAI GACNNNNGTC 1 cut(s) 2721
Psp124BI GAGCTC 2 cut(s) 413, 2518
Psp6I CCWGG 5 cut(s) 232, 2344, 2384, 2829, 2977
PspEI GGTNACC 1 cut(s) 2688
PspGI CCWGG 5 cut(s) 232, 2344, 2384, 2829, 2977
PspN4I GGNNCC 5 cut(s) 45, 456, 1142, 1625, 2037
PspOMI GGGCCC 1 cut(s) 43
PspPI GGNCC 4 cut(s) 43, 44, 1160, 1199
PspXI VCTCGAGB 1 cut(s) 50
PsrI GAACNNNNNNTAC 2 cut(s) 843, 875
PstI CTGCAG 2 cut(s) 1375, 2767
PstNI CAGNNNCTG 5 cut(s) 103, 569, 592, 1413, 2771
PsuI RGATCY 5 cut(s) 1140, 1623, 2312, 2527, 3022
PsyI GACNNNGTC 1 cut(s) 1551
PvuII CAGCTG 1 cut(s) 1214
RsaI GTAC 6 cut(s) 179, 825, 852, 1040, 1093, 1336
RsaNI GTAC 6 cut(s) 178, 824, 851, 1039, 1092, 1335
RseI CAYNNNNRTG 4 cut(s) 468, 1047, 2885, 2952
SacI GAGCTC 2 cut(s) 413, 2518
SalI GTCGAC 1 cut(s) 1458
SapI GCTCTTC 1 cut(s) 2740
Sau96I GGNCC 4 cut(s) 43, 44, 1160, 1199
ScaI AGTACT 1 cut(s) 1093
SchI GAGTC 3 cut(s) 809, 1325, 3024
ScrFI CCNGG 6 cut(s) 42, 234, 2346, 2386, 2831, 2979
SduI GDGCHC 4 cut(s) 47, 413, 1497, 2518
SfaNI GCATC 4 cut(s) 511, 955, 1471, 2828
SfcI CTRYAG 4 cut(s) 1371, 1593, 2219, 2763
Sfr274I CTCGAG 2 cut(s) 50, 2364
SinI GGWCC 2 cut(s) 1160, 1199
SlaI CTCGAG 2 cut(s) 50, 2364
SmiMI CAYNNNNRTG 4 cut(s) 468, 1047, 2885, 2952
SmlI CTYRAG 6 cut(s) 50, 1629, 2021, 2364, 2857, 3026
SmoI CTYRAG 6 cut(s) 50, 1629, 2021, 2364, 2857, 3026
SseBI AGGCCT 2 cut(s) 1329, 2096
SsiI CCGC 7 cut(s) 47, 638, 1649, 1857, 2399, 3052, 3086
SspMI CTAG 9 cut(s) 222, 801, 1271, 2394, 2469, 2739, 2910, 3099, 3141
SstI GAGCTC 2 cut(s) 413, 2518
StuI AGGCCT 2 cut(s) 1329, 2096
StyD4I CCNGG 6 cut(s) 40, 232, 2344, 2384, 2829, 2977
StyI CCWWGG 4 cut(s) 1111, 1202, 1304, 2849
TaiI ACGT 4 cut(s) 1017, 1730, 2447, 2725
TaqI TCGA 9 cut(s) 51, 625, 1342, 1459, 1730, 2059, 2365, 2545, 3046
TatI WGTACW 2 cut(s) 850, 1091
TauI GCSGC 2 cut(s) 1860, 3089
TfiI GAWTC 5 cut(s) 161, 566, 839, 2154, 2709
TseFI GTSAC 4 cut(s) 93, 713, 1219, 2714
TseI GCWGC 8 cut(s) 890, 1370, 1508, 1874, 2027, 2426, 2633, 2765
Tsp45I GTSAC 4 cut(s) 93, 713, 1219, 2714
TspGWI ACGGA 4 cut(s) 827, 1322, 2778, 3139
Tth111I GACNNNGTC 1 cut(s) 1551
Van91I CCANNNNNTGG 2 cut(s) 1137, 2309
VpaK11BI GGWCC 2 cut(s) 1160, 1199
XapI RAATTY 6 cut(s) 131, 1019, 1173, 1678, 1799, 2893
XbaI TCTAGA 1 cut(s) 221
XceI RCATGY 3 cut(s) 890, 1032, 1545
XhoI CTCGAG 2 cut(s) 50, 2364
XmiI GTMKAC 1 cut(s) 1459
XmnI GAANNNNTTC 4 cut(s) 165, 459, 2359, 2499
XspI CTAG 9 cut(s) 222, 801, 1271, 2394, 2469, 2739, 2910, 3099, 3141
ZrmI AGTACT 1 cut(s) 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.