Rh1CG060400

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0003738
Physical Location & Seq
Reverse (-)
11989068 .. 12001108
12041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG060400.1

Sequence Viewer

Length: 3204 bp
ATGTTGCGCATGGAGAGGATGACTGCCGATCAGGCGAAGGCGGGCCCACTTGAAAGGGACATTCATTTGGCCATCACTGCTCTAAAAAAGGGGTCACAATTACTGAAGTATGGACGCAGAGGAAAGCCCAAATTTTGTCCTTTCAGACTGTCCAATGATGAATCTGTTCTGACATGGTACTCAGGGAAAGATGAGAAATATGTTAAACTAAGCCATGTCTCTAGAATTATACCTGGCCAACGCACTCCAATCTTTCAAAGGCATCCTCGGCCTGAGAAGGAATATCAGTCATTTTCTCTTATATATGACGACAGGTCTTTAGATTTGATATGTAAGGATAAAGATGAAGCTGAAGTCTGGTTTACTGGTCTAAAAGCATTAATATCACGTGGCCTTCAACAGAAGGGAAGAGCAGAAACGAAAAGTGGAGTTACCTCTGAAGCAACTAGTCCAAGATCACACACGCAAAGAAGTTCTCCTTTGAGCTCTCCATTTTGTAGTGGTGATAGTTCACAGAAGGATGAAGTGGAACCCTTTCATCCTCATGCTACAGCTGAAAGCCCTCCTAAAGTCCATTTGGAGAAGGCTTTATCAGATGCCATGTTATATGCCCTCCCTCCGAAAGTTCCCTTTCCTTCAGATTCTGCTTCTGGCTCATTTCAGTCTCTGTCATCAGGAGGCTCAGATGGAATGAATGGTCGAACAAAGGGTGCAGGGACGGATGCTTTTAGAGTTAGTTTATCAAGTGCAGTTAGCTCATCAAGTCAAGGCTCTGGTCATGATGATGGTGATGCTTTAGGGGACGTTTTCATTTGGGGGGAAGGCGTAGGTGATTGCATTTTGGGTGGTGGATTACATAGAGTTGGTAGTTCTTCTAGTTCTAAGACGGACTCTTTTGTACCTAAAGTCTTGGAATCTGCTGTTGTACTTGATGTTCAAAGTATAGCTTGTGGGGGAAGACATGCTGCTTTTGTAACCAAGCAAGGGGAGGTATTTTCTTGGGGTGAAGAATTAGGAGGCAGACTCGGACATGGTGTAGATGCTGATATCTCCCAACCAAAGCTCATAGAAGGTCTCAAAAATCTTAATGTAGAATTTTTAGCCTGTGGGGAGTACCATTCTTGTGCAGTAACACTCTCTGGTGACATATACACATGGGGTGGCAGTACTTCTAATTTTGCCCTCCTTGGGCATGGGTTTCAATCGAGTCTGTGGGTCCCAAAAAAGTTAAATGGACCTTTAGAGGGAATTCATGTCTCTTCAGTTTCTTGTGGACCATGGCACACAGCCGTTGTGACCTCTGCTGGTCAATTGTTTACTTTTGGTGATGGAACTTTTGGTGTTCTAGGTCATGGGGATCGCAAGAGTATATCAGTTCCAAGGGAAGTTGAGTCCCTTAAAGGCCTTCGTACTGTTCGAGTAGCTTGTGGTGTTTGGCACTCTGCAGCAGTAATTGAAGTAATGGTGGGATCATCAAGTTCCAGCAACTGTTCTTCTGGGAAGCTTTTTACATGGGGAGATGGAGATAAAGGTCGACTTGGGCATGGTGATAAGGATGCAAGACTTGTGCCCACTTGCGTTGCTGCTTTGGTTGACCCGAACTTCTGTCAAGTTGCGTGTGGACAAAGTCTGACTGTTGCGCTTACAACAACTGGCCATGTTTACACAATGGGAAGCCCTGTTTTTGGTCAATTAGGGATCCCTCAAGCTGATGGGAAACTCCCCCACCGTGTTGAGGGAAAGCTAATGAAGAATTTTGTTGAAGAAATTGCTTGTGGTGCTTATCATGTTGCAGTCTTAACTTCGAGAACTGAAGTTTACACTTGGGGAAAGGGTGCAAATGGTAGATTAGGTCTTGGGGATACAGAGGATAAATTTTCACCAACATTAGTTGAAGCTCTAAGAGACAAACAAGTCAAAAGTATCGTTTGTGGCACTAACTTTACAGCAGCCATCTGTCTTCATAAATGGGTCTCTGGTGTTGATCAATCAATGTGTTCTGGTTGTCGCCTTCCGTTTAATTTTAAAAGAAAAAGACACAACTGCTACAATTGTGGACTTGTATTATGCCATTCATGCAGTAGCAAAAAGTGCCTCAAGGCTTCAATGGCACCAAATCCAAACAAACCATATCGAGTTTGTGATAATTGTTTTGGGAAACTCAGAAAGGCCTCTGAAACCAACTCTTCATCTGGCTCCGCTTTGAAAAAAGGAAGTAATCAGGGATTTGCTGAACTGAGTGAAAAAAATATTAAAGATTTGGACACTGGTTCATCCACAAGAAACAAGAAACTAGATTTTAGCAGTAATTGTGTTGCTCCCTTTCCAAATGGCATTTCTGAGCGCCGTGCATTCAGTGGGACTAAATCTTTCAACCCAGTATTTGGATCTTCCAAAAAGTTTTTCTCAGCTTCTCTTCCTGGATCAAGAATTGCTTCTCGAGCAACATCACCAACATCGAGACGATCTAGTCCACCTCGTGCCAAAACGCCAACCCCTGCTCTCTCAGGGATCACATTGCCAAATGTTGTGGAATATGCTAGAGGGACAAATGACGGTCTAAGTGAAGAAATTATTAAACTAAGAGCTCAGGTCGAAGATCTTACTCGTAAAAGTCGAGTTCAAGATACTGAGCTGGAAAGAACTACCCACCAATTGAAGGAGGCTGTCAAAGTAGCAGACGAAGAAACTGCAAAATGTAAAGCAGCAAAGGAAGTAATTAAGTCGCTCACAGCTCAATTAAAGGAAATGGCTGAAAGGTTACCCATTGGGACAGCAAGAAGCAGCGACACACGTCCATTAGGCTTTTCTAGTCCTACCCATGACGTTTCTACTACAGCCATTGAGAAAGCAAGCAGTCCATTGACCTTCCATGAACCACATTCAATTGCATCAAATAGCCTGGTGGTTTCTAATGCATCAAGGATTTTGAGTAATCATGCTTCAAATCCCACTGAAGTGGCAAATTCGGAAGCAACTACTAGGCATAAGAACAGAGGAACAAAAGCTGAAACCACAAATGGGGATGAATGGGTTGAGCAAGATGAGCCTGGTGTATATATTACCCTGGTTTCCTTGCCCACAGGAGTCAAAGATCTCAAGCGTGTCCGCTTCAGGCATGTCTCTTTTCCTAATTATTTCATTCACCCAAATTTGAAGAAAGATTCTCTGGAGCTGTATTTAATGTTTGTTGGTTTGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N BRX_assoc FYVE PH_12 RCC1

Protein Analysis

1067

Amino Acids

114.96

Weight (kDa)

9.01

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 26 - 128 3.4e-08 Pleckstrin homology domain
WD40_RLD PF25390 268 - 651 3.5e-80 RCC1-like domain
RCC1_2 PF13540 312 - 335 2.5e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 328 - 377 6.7e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 367 - 390 4.1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 380 - 432 8.2e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 419 - 447 8.2e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 436 - 484 4.4e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 499 - 548 6.2e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 552 - 600 1.2e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 605 - 652 1.5e-14 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 656 - 722 2.2e-14 FYVE zinc finger
BRX_N PF13713 895 - 928 1.2e-14 Transcription factor BRX N-terminal domain
BRX_assoc PF16627 935 - 999 1.1e-10 Unstructured region between BRX_N and BRX domain
BRX PF08381 1009 - 1043 6.1e-12 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1913, 2469
Acc16I TGCGCA 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 2110
AccB7I CCANNNNNTGG 1 cut(s) 2384
AccI GTMKAC 1 cut(s) 1534
AciI CCGC 3 cut(s) 41, 2199, 3109
AclWI GGATC 7 cut(s) 1365, 1477, 1693, 1706, 2395, 2431, 2519
AcoI YGGCCR 3 cut(s) 69, 235, 1654
AcsI RAATTY 7 cut(s) 131, 1094, 1248, 1753, 1874, 2965, 3151
AcuI CTGAAG 8 cut(s) 125, 372, 459, 621, 1245, 1833, 2976, 3097
AcvI CACGTG 1 cut(s) 389
AdeI CACNNNGTG 1 cut(s) 2480
AfaI GTAC 6 cut(s) 179, 900, 927, 1115, 1168, 1411
AfiI CCNNNNNNNGG 8 cut(s) 984, 1188, 1244, 1685, 1735, 2384, 2659, 3021
AflIII ACRYGT 1 cut(s) 2792
AhdI GACNNNNNGTC 1 cut(s) 313
AhlI ACTAGT 1 cut(s) 446
AjiI CACGTC 1 cut(s) 2795
AjnI CCWGG 5 cut(s) 232, 2419, 2901, 3049, 3066
AjuI GAANNNNNNNTTGG 4 cut(s) 2320, 2352, 2387, 2419
AleI CACNNNNGTG 1 cut(s) 2957
Alw21I GWGCWC 2 cut(s) 488, 2590
Alw26I GTCTC 8 cut(s) 223, 669, 1079, 1261, 1899, 1978, 2455, 3127
AlwI GGATC 7 cut(s) 1365, 1477, 1693, 1706, 2395, 2431, 2519
AlwNI CAGNNNCTG 3 cut(s) 644, 667, 1488
Ama87I CYCGRG 1 cut(s) 2439
AoxI GGCC 8 cut(s) 43, 69, 235, 269, 391, 1402, 1654, 2169
ApaI GGGCCC 1 cut(s) 47
ApeKI GCWGC 6 cut(s) 965, 1445, 1583, 1949, 2705, 2784
ApoI RAATTY 7 cut(s) 131, 1094, 1248, 1753, 1874, 2965, 3151
AseI ATTAAT 1 cut(s) 380
Asp700I GAANNNNTTC 3 cut(s) 165, 534, 2434
AspLEI GCGC 3 cut(s) 9, 1642, 2346
AspS9I GGNCC 5 cut(s) 43, 44, 1216, 1235, 1274
AvaI CYCGRG 1 cut(s) 2439
AvaII GGWCC 3 cut(s) 1216, 1235, 1274
BaeGI GKGCMC 2 cut(s) 47, 1572
BalI TGGCCA 3 cut(s) 71, 237, 1656
BamHI GGATCC 1 cut(s) 1698
BanI GGYRCC 1 cut(s) 2110
BanII GRGCYC 3 cut(s) 47, 488, 2590
BarI GAAGNNNNNNTAC 2 cut(s) 2926, 2958
BauI CACGAG 1 cut(s) 2478
BbrPI CACGTG 1 cut(s) 389
BbsI GAAGAC 2 cut(s) 964, 1952
Bbv12I GWGCWC 2 cut(s) 488, 2590
BbvI GCAGC 6 cut(s) 952, 1457, 1570, 1961, 2717, 2796
BccI CCATC 7 cut(s) 80, 680, 779, 1322, 1514, 1706, 1961
BceAI ACGGC 2 cut(s) 1274, 2331
BcgI CGANNNNNNTGC 2 cut(s) 2672, 2706
BciT130I CCWGG 5 cut(s) 234, 2421, 2903, 3051, 3068
BciVI GTATCC 1 cut(s) 1855
BclI TGATCA 1 cut(s) 1984
BcoDI GTCTC 8 cut(s) 223, 669, 1079, 1261, 1899, 1978, 2455, 3127
BcuI ACTAGT 1 cut(s) 446
BfaI CTAG 9 cut(s) 222, 447, 876, 1346, 2294, 2469, 2541, 2811, 2982
BfmI CTRYAG 3 cut(s) 549, 1443, 2835
BfoI RGCGCY 1 cut(s) 2347
BfuI GTATCC 1 cut(s) 1855
BglI GCCNNNNNGGC 1 cut(s) 32
BglII AGATCT 2 cut(s) 2599, 3094
BisI GCNGC 6 cut(s) 966, 1446, 1584, 1950, 2706, 2785
BlsI GCNGC 6 cut(s) 967, 1447, 1585, 1951, 2707, 2786
BmcAI AGTACT 1 cut(s) 1168
Bme1390I CCNGG 5 cut(s) 234, 2421, 2903, 3051, 3068
Bme18I GGWCC 3 cut(s) 1216, 1235, 1274
BmeRI GACNNNNNGTC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 2439
BmgBI CACGTC 1 cut(s) 2795
BmgT120I GGNCC 5 cut(s) 43, 44, 1216, 1235, 1274
BmiI GGNNCC 7 cut(s) 45, 531, 1217, 1218, 1700, 2112, 2197
BmrFI CCNGG 5 cut(s) 234, 2421, 2903, 3051, 3068
BmrI ACTGGG 1 cut(s) 2372
BmsI GCATC 8 cut(s) 271, 586, 712, 781, 1030, 1546, 2900, 2927
BmuI ACTGGG 1 cut(s) 2372
BoxI GACNNNNGTC 1 cut(s) 2793
BpiI GAAGAC 2 cut(s) 964, 1952
BplI GAGNNNNNCTC 2 cut(s) 1008, 1040
BpmI CTGGAG 1 cut(s) 3191
Bpu10I CCTNAGC 1 cut(s) 2589
BpuEI CTTGAG 3 cut(s) 1689, 2081, 3083
BsaAI YACGTR 1 cut(s) 389
BsaI GGTCTC 2 cut(s) 1079, 1978
BsaJI CCNNGG 5 cut(s) 266, 1186, 1277, 1379, 3066
Bsc4I CCNNNNNNNGG 8 cut(s) 984, 1188, 1244, 1685, 1735, 2384, 2659, 3021
Bse1I ACTGG 4 cut(s) 370, 1657, 2272, 2378
Bse3DI GCAATG 1 cut(s) 2516
BseBI CCWGG 5 cut(s) 234, 2421, 2903, 3051, 3068
BseDI CCNNGG 5 cut(s) 266, 1186, 1277, 1379, 3066
BseGI GGATG 8 cut(s) 24, 262, 526, 538, 727, 1561, 2273, 3031
BseLI CCNNNNNNNGG 8 cut(s) 984, 1188, 1244, 1685, 1735, 2384, 2659, 3021
BseMI GCAATG 1 cut(s) 2516
BseNI ACTGG 4 cut(s) 370, 1657, 2272, 2378
BseSI GKGCMC 2 cut(s) 47, 1572
BseXI GCAGC 6 cut(s) 952, 1457, 1570, 1961, 2717, 2796
BsgI GTGCAG 3 cut(s) 732, 768, 1146
BshFI GGCC 8 cut(s) 45, 71, 237, 271, 393, 1404, 1656, 2171
BshNI GGYRCC 1 cut(s) 2110
BsiHKAI GWGCWC 2 cut(s) 488, 2590
BsiHKCI CYCGRG 1 cut(s) 2439
BslFI GGGAC 8 cut(s) 71, 730, 815, 1202, 1378, 2374, 2560, 2785
BslI CCNNNNNNNGG 8 cut(s) 984, 1188, 1244, 1685, 1735, 2384, 2659, 3021
BsmAI GTCTC 8 cut(s) 223, 669, 1079, 1261, 1899, 1978, 2455, 3127
BsmBI CGTCTC 1 cut(s) 2455
BsmFI GGGAC 8 cut(s) 71, 730, 815, 1202, 1378, 2374, 2560, 2785
BsmI GAATGC 1 cut(s) 2351
BsnI GGCC 8 cut(s) 45, 71, 237, 271, 393, 1404, 1656, 2171
Bso31I GGTCTC 2 cut(s) 1079, 1978
BsoBI CYCGRG 1 cut(s) 2439
Bsp120I GGGCCC 1 cut(s) 43
Bsp1286I GDGCHC 4 cut(s) 47, 488, 1572, 2590
Bsp19I CCATGG 1 cut(s) 1277
BspACI CCGC 3 cut(s) 41, 2199, 3109
BspANI GGCC 8 cut(s) 45, 71, 237, 271, 393, 1404, 1656, 2171
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 7 cut(s) 45, 531, 1217, 1218, 1700, 2112, 2197
BspMAI CTGCAG 1 cut(s) 1447
BspPI GGATC 7 cut(s) 1365, 1477, 1693, 1706, 2395, 2431, 2519
BspQI GCTCTTC 1 cut(s) 403
BspT107I GGYRCC 1 cut(s) 2110
BspTNI GGTCTC 2 cut(s) 1079, 1978
BsrDI GCAATG 1 cut(s) 2516
BsrI ACTGG 4 cut(s) 370, 1657, 2272, 2378
BssECI CCNNGG 5 cut(s) 266, 1186, 1277, 1379, 3066
BssSI CACGAG 1 cut(s) 2478
BssT1I CCWWGG 3 cut(s) 1186, 1277, 1379
Bst2BI CACGAG 1 cut(s) 2478
Bst2UI CCWGG 5 cut(s) 234, 2421, 2903, 3051, 3068
Bst4CI ACNGT 6 cut(s) 150, 1414, 1490, 1636, 1730, 2558
Bst6I CTCTTC 4 cut(s) 403, 1264, 2191, 2421
BstAPI GCANNNNNTGC 1 cut(s) 2091
BstBAI YACGTR 1 cut(s) 389
BstC8I GCNNGC 2 cut(s) 43, 2854
BstDSI CCRYGG 1 cut(s) 1277
BstEII GGTNACC 1 cut(s) 2760
BstF5I GGATG 8 cut(s) 24, 262, 526, 538, 727, 1561, 2273, 3031
BstH2I RGCGCY 1 cut(s) 2347
BstHHI GCGC 3 cut(s) 9, 1642, 2346
BstMAI GTCTC 8 cut(s) 223, 669, 1079, 1261, 1899, 1978, 2455, 3127
BstMWI GCNNNNNNNGC 9 cut(s) 32, 77, 268, 1778, 2076, 2091, 2108, 2441, 3046
BstNI CCWGG 5 cut(s) 234, 2421, 2903, 3051, 3068
BstNSI RCATGY 2 cut(s) 965, 3122
BstPAI GACNNNNGTC 1 cut(s) 2793
BstPI GGTNACC 1 cut(s) 2760
BstSCI CCNGG 5 cut(s) 232, 2419, 2901, 3049, 3066
BstSFI CTRYAG 3 cut(s) 549, 1443, 2835
BstSLI GKGCMC 2 cut(s) 47, 1572
BstV1I GCAGC 6 cut(s) 952, 1457, 1570, 1961, 2717, 2796
BstV2I GAAGAC 2 cut(s) 964, 1952
BstX2I RGATCY 4 cut(s) 1698, 2387, 2599, 3094
BstXI CCANNNNNNTGG 1 cut(s) 2959
BstYI RGATCY 4 cut(s) 1698, 2387, 2599, 3094
BsuI GTATCC 1 cut(s) 1855
BsuRI GGCC 8 cut(s) 45, 71, 237, 271, 393, 1404, 1656, 2171
BtgI CCRYGG 1 cut(s) 1277
BtrI CACGTC 1 cut(s) 2795
BtsCI GGATG 8 cut(s) 24, 262, 526, 538, 727, 1561, 2273, 3031
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 4 cut(s) 75, 2265, 2362, 2952
Cac8I GCNNGC 2 cut(s) 43, 2854
CaiI CAGNNNCTG 3 cut(s) 644, 667, 1488
CciI TCATGA 1 cut(s) 778
CfoI GCGC 3 cut(s) 9, 1642, 2346
Cfr13I GGNCC 5 cut(s) 43, 44, 1216, 1235, 1274
CseI GACGC 1 cut(s) 123
Csp6I GTAC 6 cut(s) 178, 899, 926, 1114, 1167, 1410
CspCI CAANNNNNGTGG 4 cut(s) 1561, 1596, 2511, 2546
CviQI GTAC 6 cut(s) 178, 899, 926, 1114, 1167, 1410
DraI TTTAAA 1 cut(s) 2026
DraIII CACNNNGTG 1 cut(s) 2480
DrdI GACNNNNNNGTC 2 cut(s) 1913, 2469
DriI GACNNNNNGTC 1 cut(s) 313
DseDI GACNNNNNNGTC 2 cut(s) 1913, 2469
EaeI YGGCCR 3 cut(s) 69, 235, 1654
Eam1104I CTCTTC 4 cut(s) 403, 1264, 2191, 2421
Eam1105I GACNNNNNGTC 1 cut(s) 313
EarI CTCTTC 4 cut(s) 403, 1264, 2191, 2421
Ecl136II GAGCTC 2 cut(s) 486, 2588
Eco130I CCWWGG 3 cut(s) 1186, 1277, 1379
Eco147I AGGCCT 2 cut(s) 1404, 2171
Eco24I GRGCYC 3 cut(s) 47, 488, 2590
Eco31I GGTCTC 2 cut(s) 1079, 1978
Eco32I GATATC 1 cut(s) 1048
Eco47I GGWCC 3 cut(s) 1216, 1235, 1274
Eco53kI GAGCTC 2 cut(s) 486, 2588
Eco57I CTGAAG 8 cut(s) 125, 372, 459, 621, 1245, 1833, 2976, 3097
Eco72I CACGTG 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 2439
Eco91I GGTNACC 1 cut(s) 2760
EcoICRI GAGCTC 2 cut(s) 486, 2588
EcoO109I RGGNCCY 1 cut(s) 1216
EcoO65I GGTNACC 1 cut(s) 2760
EcoRI GAATTC 1 cut(s) 1248
EcoRII CCWGG 5 cut(s) 232, 2419, 2901, 3049, 3066
EcoRV GATATC 1 cut(s) 1048
EcoT14I CCWWGG 3 cut(s) 1186, 1277, 1379
EcoT22I ATGCAT 1 cut(s) 2920
EcoT38I GRGCYC 3 cut(s) 47, 488, 2590
ErhI CCWWGG 3 cut(s) 1186, 1277, 1379
Esp3I CGTCTC 1 cut(s) 2455
FalI AAGNNNNNCTT 8 cut(s) 463, 495, 931, 963, 1521, 1553, 2419, 2451
FaqI GGGAC 8 cut(s) 71, 730, 815, 1202, 1378, 2374, 2560, 2785
FauI CCCGC 1 cut(s) 34
FbaI TGATCA 1 cut(s) 1984
FblI GTMKAC 1 cut(s) 1534
Fnu4HI GCNGC 6 cut(s) 966, 1446, 1584, 1950, 2706, 2785
FokI GGATG 8 cut(s) 31, 249, 525, 533, 734, 1568, 2260, 3038
FriOI GRGCYC 3 cut(s) 47, 488, 2590
Fsp4HI GCNGC 6 cut(s) 966, 1446, 1584, 1950, 2706, 2785
FspBI CTAG 9 cut(s) 222, 447, 876, 1346, 2294, 2469, 2541, 2811, 2982
FspI TGCGCA 1 cut(s) 8
GlaI GCGC 3 cut(s) 8, 1641, 2345
GluI GCNGC 6 cut(s) 966, 1446, 1584, 1950, 2706, 2785
GsuI CTGGAG 1 cut(s) 3191
HaeII RGCGCY 1 cut(s) 2347
HaeIII GGCC 8 cut(s) 45, 71, 237, 271, 393, 1404, 1656, 2171
HgaI GACGC 1 cut(s) 123
HhaI GCGC 3 cut(s) 9, 1642, 2346
Hin6I GCGC 3 cut(s) 7, 1640, 2344
HinP1I GCGC 3 cut(s) 7, 1640, 2344
HincII GTYRAC 2 cut(s) 1535, 1594
HindII GTYRAC 2 cut(s) 1535, 1594
HindIII AAGCTT 1 cut(s) 1502
HinfI GANTC 9 cut(s) 161, 641, 890, 914, 1023, 1207, 1391, 3087, 3164
Hpy188III TCNNGA 9 cut(s) 222, 675, 779, 1806, 2427, 2439, 2460, 2624, 3170
HpyCH4III ACNGT 6 cut(s) 150, 1414, 1490, 1636, 1730, 2558
HpyCH4IV ACGT 4 cut(s) 388, 804, 2794, 2826
HpyF10VI GCNNNNNNNGC 9 cut(s) 32, 77, 268, 1778, 2076, 2091, 2108, 2441, 3046
HpySE526I ACGT 4 cut(s) 388, 804, 2794, 2826
HspAI GCGC 3 cut(s) 7, 1640, 2344
KflI GGGWCCC 1 cut(s) 1216
Ksp22I TGATCA 1 cut(s) 1984
LguI GCTCTTC 1 cut(s) 403
LmnI GCTCC 3 cut(s) 2201, 2323, 3172
Lsp1109I GCAGC 6 cut(s) 952, 1457, 1570, 1961, 2717, 2796
LweI GCATC 8 cut(s) 271, 586, 712, 781, 1030, 1546, 2900, 2927
MaeI CTAG 9 cut(s) 222, 447, 876, 1346, 2294, 2469, 2541, 2811, 2982
MaeII ACGT 4 cut(s) 388, 804, 2794, 2826
MaeIII GTNAC 7 cut(s) 93, 430, 973, 1129, 1142, 1294, 2760
MfeI CAATTG 4 cut(s) 1310, 2050, 2654, 2886
MflI RGATCY 4 cut(s) 1698, 2387, 2599, 3094
MhlI GDGCHC 4 cut(s) 47, 488, 1572, 2590
MlsI TGGCCA 3 cut(s) 71, 237, 1656
MluNI TGGCCA 3 cut(s) 71, 237, 1656
MlyI GAGTC 5 cut(s) 884, 1017, 1216, 1400, 3096
Mox20I TGGCCA 3 cut(s) 71, 237, 1656
Mph1103I ATGCAT 1 cut(s) 2920
MroXI GAANNNNTTC 3 cut(s) 165, 534, 2434
MscI TGGCCA 3 cut(s) 71, 237, 1656
MslI CAYNNNNRTG 4 cut(s) 543, 783, 1122, 2957
Msp20I TGGCCA 3 cut(s) 71, 237, 1656
MspA1I CMGCKG 1 cut(s) 554
MspR9I CCNGG 5 cut(s) 234, 2421, 2903, 3051, 3068
MunI CAATTG 4 cut(s) 1310, 2050, 2654, 2886
Mva1269I GAATGC 1 cut(s) 2351
MvaI CCWGG 5 cut(s) 234, 2421, 2903, 3051, 3068
MwoI GCNNNNNNNGC 9 cut(s) 32, 77, 268, 1778, 2076, 2091, 2108, 2441, 3046
NcoI CCATGG 1 cut(s) 1277
NlaIV GGNNCC 7 cut(s) 45, 531, 1217, 1218, 1700, 2112, 2197
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 3 cut(s) 93, 1142, 1294
NsbI TGCGCA 1 cut(s) 8
NsiI ATGCAT 1 cut(s) 2920
NspI RCATGY 2 cut(s) 965, 3122
OliI CACNNNNGTG 1 cut(s) 2957
PaeR7I CTCGAG 1 cut(s) 2439
PagI TCATGA 1 cut(s) 778
PceI AGGCCT 2 cut(s) 1404, 2171
PciSI GCTCTTC 1 cut(s) 403
PcsI WCGNNNNNNNCGW 2 cut(s) 1414, 2614
PctI GAATGC 1 cut(s) 2351
PdmI GAANNNNTTC 3 cut(s) 165, 534, 2434
PfeI GAWTC 4 cut(s) 161, 641, 914, 3164
PflFI GACNNNGTC 1 cut(s) 1626
PflMI CCANNNNNTGG 1 cut(s) 2384
PfoI TCCNGGA 1 cut(s) 2419
PkrI GCNGC 6 cut(s) 967, 1447, 1585, 1951, 2707, 2786
PleI GAGTC 5 cut(s) 884, 1017, 1215, 1399, 3095
PmaCI CACGTG 1 cut(s) 389
PmlI CACGTG 1 cut(s) 389
PpsI GAGTC 5 cut(s) 884, 1017, 1215, 1399, 3095
Ppu21I YACGTR 1 cut(s) 389
PpuMI RGGWCCY 1 cut(s) 1216
PshAI GACNNNNGTC 1 cut(s) 2793
PshBI ATTAAT 1 cut(s) 380
Psp124BI GAGCTC 2 cut(s) 488, 2590
Psp5II RGGWCCY 1 cut(s) 1216
Psp6I CCWGG 5 cut(s) 232, 2419, 2901, 3049, 3066
PspCI CACGTG 1 cut(s) 389
PspEI GGTNACC 1 cut(s) 2760
PspGI CCWGG 5 cut(s) 232, 2419, 2901, 3049, 3066
PspN4I GGNNCC 7 cut(s) 45, 531, 1217, 1218, 1700, 2112, 2197
PspOMI GGGCCC 1 cut(s) 43
PspPI GGNCC 5 cut(s) 43, 44, 1216, 1235, 1274
PspPPI RGGWCCY 1 cut(s) 1216
PsrI GAACNNNNNNTAC 2 cut(s) 918, 950
PstI CTGCAG 1 cut(s) 1447
PstNI CAGNNNCTG 3 cut(s) 644, 667, 1488
PsuI RGATCY 4 cut(s) 1698, 2387, 2599, 3094
PsyI GACNNNGTC 1 cut(s) 1626
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 6 cut(s) 179, 900, 927, 1115, 1168, 1411
RsaNI GTAC 6 cut(s) 178, 899, 926, 1114, 1167, 1410
RseI CAYNNNNRTG 4 cut(s) 543, 783, 1122, 2957
SacI GAGCTC 2 cut(s) 488, 2590
SalI GTCGAC 1 cut(s) 1533
SapI GCTCTTC 1 cut(s) 403
SatI GCNGC 6 cut(s) 966, 1446, 1584, 1950, 2706, 2785
Sau96I GGNCC 5 cut(s) 43, 44, 1216, 1235, 1274
ScaI AGTACT 1 cut(s) 1168
SchI GAGTC 5 cut(s) 884, 1017, 1216, 1400, 3096
ScrFI CCNGG 5 cut(s) 234, 2421, 2903, 3051, 3068
SduI GDGCHC 4 cut(s) 47, 488, 1572, 2590
SfaNI GCATC 8 cut(s) 271, 586, 712, 781, 1030, 1546, 2900, 2927
SfcI CTRYAG 3 cut(s) 549, 1443, 2835
Sfr274I CTCGAG 1 cut(s) 2439
SinI GGWCC 3 cut(s) 1216, 1235, 1274
SlaI CTCGAG 1 cut(s) 2439
SmiMI CAYNNNNRTG 4 cut(s) 543, 783, 1122, 2957
SmlI CTYRAG 4 cut(s) 1704, 2096, 2439, 3098
SmoI CTYRAG 4 cut(s) 1704, 2096, 2439, 3098
SpeI ACTAGT 1 cut(s) 446
SseBI AGGCCT 2 cut(s) 1404, 2171
SsiI CCGC 3 cut(s) 41, 2199, 3109
SspI AATATT 1 cut(s) 2251
SspMI CTAG 9 cut(s) 222, 447, 876, 1346, 2294, 2469, 2541, 2811, 2982
SstI GAGCTC 2 cut(s) 488, 2590
StuI AGGCCT 2 cut(s) 1404, 2171
StyD4I CCNGG 5 cut(s) 232, 2419, 2901, 3049, 3066
StyI CCWWGG 3 cut(s) 1186, 1277, 1379
TaaI ACNGT 6 cut(s) 150, 1414, 1490, 1636, 1730, 2558
TaiI ACGT 4 cut(s) 391, 807, 2797, 2829
TatI WGTACW 2 cut(s) 925, 1166
TfiI GAWTC 4 cut(s) 161, 641, 914, 3164
TscAI CASTG 4 cut(s) 82, 2272, 2362, 2959
TseFI GTSAC 3 cut(s) 93, 1142, 1294
TseI GCWGC 6 cut(s) 965, 1445, 1583, 1949, 2705, 2784
Tsp45I GTSAC 3 cut(s) 93, 1142, 1294
TspGWI ACGGA 3 cut(s) 734, 902, 2004
TspRI CASTG 4 cut(s) 82, 2272, 2362, 2959
Tth111I GACNNNGTC 1 cut(s) 1626
Van91I CCANNNNNTGG 1 cut(s) 2384
VpaK11BI GGWCC 3 cut(s) 1216, 1235, 1274
VspI ATTAAT 1 cut(s) 380
XapI RAATTY 7 cut(s) 131, 1094, 1248, 1753, 1874, 2965, 3151
XbaI TCTAGA 1 cut(s) 221
XceI RCATGY 2 cut(s) 965, 3122
XhoI CTCGAG 1 cut(s) 2439
XmiI GTMKAC 1 cut(s) 1534
XmnI GAANNNNTTC 3 cut(s) 165, 534, 2434
XspI CTAG 9 cut(s) 222, 447, 876, 1346, 2294, 2469, 2541, 2811, 2982
ZrmI AGTACT 1 cut(s) 1168
Zsp2I ATGCAT 1 cut(s) 2920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.