Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp02

Orthogroup ID

OG0003738

Physical Location & Seq

Genomic Coordinates Reverse (-)

3724222 .. 3733825

Gene Structure 9604 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.2G034600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3258 bp

ATGTTGCGCATGGAGAGGATGACTGCGGATCAGGCGAAGGCGGGCCCACTCGAAAGGGACATTGACTTGCAGGCCATCACTGCTCTGAAAAAAGGTGCACAGTTACTCAAGTATGGACGCAGAGGAAAACCCAAATTTTGTCCTTTCAGGCTGTCCAATGATGAATCTTCTCTAATATGGTACTCAGGGAAAGAGGAGAAATATGTTAAACTAAGCCACGTCTCCAGAATTATACCTGGTCAGCGAACACAAACCTTTCAGAGGTATCCTCGACCTGAAAAGGAATATCAGTCATTTTCTCTTATGTATAATGATCGGTCTTTAGATTTGATATGCAAGGATAAAGATGAAGCTGAAGTTTGGTTTACTGGTCTAAAAGCGTTAATATCACGTGGCCTTCATCAGAAGGGGAGAGCAGAATCAAGAAGTGGAGTTACATCTGAAGCAAATAGTCCGAGATCACATACCCAAAGAAGTTCTCCTTTGAGCTCTCCATTTTGTAGTGGTGATAGTTCGCAGAAGGATGGTGTGGAACCCTTTCATCTTCAAGCTACATTTGAAAGCCCTCCAAAAGTTGGTTTGGAGAAGGCTTTATCAGATGTCATGTTATATGCCCTGCCTCCAAAAATTCCATTTCCTTCAGACACTGCATGTGGCTCGGTCCAGTCTCTGTCATCAGGAGGTTCAGATGGAATGAATGGACGAATAAAGGGTGCAGGTGTGGATGCTTTTAGAGTAAGTTTATCAAGTGCTGTTAGCTCATCAAGTCAAGGCTCTGGTCATGATGATGGTGATGCTTTAGGGGATGTTTTCATTTGGGGGGAAGGCATTGGTGATTGCTTTCTGGGTGGAGGATCACGTAGAGTTGGAAGTTCTTCTAGTTGTAAGATGGACTCCTCTGTGCCTAAAGTCTTGGAATCTGCTGTAGTACTTGATGTTCAAAGTATAGCTTGTGGTGGACGACATGCTGCTTTCGTAACCAAGCAAGGAGAGGTATTTTCTTGGGGCGAAGAGTTAGGAGGCAGACTTGGACATGGTGTGGATGCTGATGTTTCGCATCCGAAGCTCATAGATGCTCTTAAAAATATCAATGTTGAATTTTTAGCATGCGGGGAATACCACTCTTGTGCTGTAACACTTTCCGGTGACATGTACACATGGGGTGGCAGTACTTGCAATTTCTCGCTCCTTGGCCATGGGTTTCAATCAAGTCAATGGGTCCCGAGAAAGCTAAATGGACCTTTAGAGGGGATTCATGTCTCATCGGTTTCTTGTGGACCATGGCACACAGCTGTTGTGACCTCTGCTGGCCAATTGTTTACTTTTGGAGATGGAACTTTTGGTGTTCTAGGTCATGGGGATCGAAAGAGTATTCCGGTACCCAGGGAGGTTGAGTCCCTTAAAGGTCTCCGCACTGTGCGAGTAGCTTGTGGTGTTTGGCACACTGCAGCAGTGATTGAAGTTATGATGGGGTCATCAAGTTCCAGCAACTGTTCTTCTGGAAAGCTTTTTACATGGGGAGATGGAGATAAAGGTCGACTTGGACATGGTGACAAGGAAGCAAGACTTGTGCCCACTTGTGTTGCTGCTTTGGTTGAACCCAACTTCTGTAAAGTTGCTTGCGGACAAAGTCTGACTGTTGCACTTACAACTACGGGCCATGTTTACACAATGGGAAGCCCTGTTTTTGGTCAACTAGGGGTCCCTCAAGCTGATGGGAAACTTCCATGCCGTGTTGAGGGAAAGCTAATGAAGAGTTTTGTTGAAGAAATTGCTTGTGGTGCTTATCATGTTGCAGTTTTAACTTCGAGAACTGAAGTTTACACTTGGGGAAAGGGTGCAAATGGTCGATTAGGTCTTGGGGATACAGAAGATAAATTTTCGCCAACATTAGTTGAAGCTTTGAAAGACAAACAAGTCAAGAGTATTGTTTGTGGCACTAACTTTACAGCAGCTATCTGTCTTCATAAATGGGTCTCTGGTATTGATCAATCGATGTGTTCTGGCTGTCGCCTTCCATTTAATTTTAAAAGAAAAAGACACAATTGTTATAATTGTGGACTTGTGTTATGCCATTCATGCAGCAGCAAAAAGTGTCTCAAGGCTTCCATGGCACCAAACCCAAACAAACCTTATCGTGTCTGTGACAACTGTTTTAGCAAACTTAGAAAGGCAACCGAAACCAACTCTTCATCTGGGCCTGCTTTTAGTAGAAGAGGAAGTATGAATCAGGGATTCAATGAACTAATTGAAAACAATGTTAAGGAAATGGAAAGTGGATCTTCCAAAAGAAACAAGAAACTGGATTTTAGCAGCAATTGCGTTACTCCCACTCCAAATGGAGTTTCTGAGCGCCGTGCAGTCAATGCGACTAAATCTTTCAACCCAATGTTTGGATCATCCAAAAAGTTTTTCTCAGCTTCGCTTCCTGGATCCAGAATTGCATCCCGAGCAACATCACCAACATCAAGGCGATCTAGTCCACCTCGTGCAACAACCCCAACCCCAGCTCTTTCAGGGCTTACTTTGCCAAAAGTTGTGGATGATGCTAAAAGGACAAATGACAGTCTGAGTGAAGAAGTTCTTAAATTAAGAGCTCAGGTTGAAGATCTTACTCGTAAAGCTCAACTTCAAGATATTGAGATGGAACGAACAACCCAACAGTTGAAGGAGGCTCTCACAGTAGCAGGGGAAGAAACTACAAAATGTAAAGCAGCGAAGGAAGTAATTAAGTCGCTCACAGCCCAATTGAAGGAAATGGCTGAAAGGTTACCGATTGGAACAGCAAGAAACATTGATACTCGTCCATTAGACTCTCCCAGTCCCACTCATGAAGTTTCTACTGCAGCCATTGAGAAAACAAGCAGTCCATTGACTTTCCATGAACCATATTCAGTTGTATCAAACAGCTTGATGATTTCTGACTTGCCAAAGTCCTACAATAACCACACTTCAAATCACACTGAAGTGACACATCCAGAAGCAACAGCTAGGCATAAGAACAGGGGAACAAAAACTGAAGCAACACACGGGGTTGAATGGGTTGAGCAAGATGAGCCTGGTGTATATATTACCCTTGTTTCCTTGCCTGGAGGAGTCAAAGATCTCAAGCGTGTCCGCTTCAGTCGAAAGCGGTTCAGTGAGAAACAAGCAGAACAGTGGTGGGCGGCAAATAGGGGTAGAGTATATCAGCAGTACAATGTTCCGGTGGTTGAAAAACCGGGTATTCCCATGGGGAGAGAAGGGTTAGCTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRX BRX_N BRX_assoc FYVE PH_12 RCC1

Protein Analysis

1086

Amino Acids

117.63

Weight (kDa)

9.06

Isoelectric Point (pI)

44.82

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12350 AT5G19420 AT5G19420 AT5G19420
fragaria_vesca	FvH4_7g02960 FvH4_7g02960 FvH4_7g02960 FvH4_7g02960
malus_domestica	MD02G1288400.v1.1 MD07G1038100.v1.1 MD07G1038400.v1.1 MD13G1037300.v1.1
prunus_persica	Prupe.2G034600_v2.0.a1 Prupe.2G034600_v2.0.a1 Prupe.2G034600_v2.0.a1
pyrus_communis	pycom02g24410 pycom05g01140 pycom07g02640 pycom07g02670 pycom16g06500
rosa_chinensis	RchiOBHm_Chr1g0323931
rosa_laevigata	RLG00000030242
rosa_multiflora	Rmu_co8261369.1_g000001 Rmu_sc0001449.1_g000001 Rmu_sc0011010.1_g000012 Rmu_sc0012343.1_g000006 Rmu_sc0012343.1_g000007
rosa_roxburghii	Rroxscaffold_4G00326110
rosa_rugosa	Rorug01G0040600
rosa_samantha	Rh1AG058500 Rh1BG049600 Rh1CG060400 Rh1DG064400
rosa_wichuraiana	Rw0G011380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2052
AarI	CACCTGC	1 cut(s)	707
AasI	GACNNNNNNGTC	1 cut(s)	1916
Acc16I	TGCGCA	1 cut(s)	8
Acc36I	ACCTGC	1 cut(s)	707
Acc65I	GGTACC	1 cut(s)	1378
AccB1I	GGYRCC	2 cut(s)	1378, 2113
AccB7I	CCANNNNNTGG	2 cut(s)	575, 2393
AccI	GTMKAC	1 cut(s)	1537
AciI	CCGC	8 cut(s)	26, 41, 1110, 1411, 1623, 3118, 3133, 3167
AclWI	GGATC	7 cut(s)	36, 862, 1368, 2287, 2404, 2427, 2440
AcoI	YGGCCR	2 cut(s)	1192, 1309
AcsI	RAATTY	4 cut(s)	134, 627, 1097, 1877
AcuI	CTGAAG	7 cut(s)	375, 462, 624, 1836, 2985, 3039, 3106
AcvI	CACGTG	1 cut(s)	392
AdeI	CACNNNGTG	1 cut(s)	2489
AfaI	GTAC	6 cut(s)	182, 930, 1154, 1171, 1380, 3197
AfiI	CCNNNNNNNGG	7 cut(s)	261, 575, 1247, 1688, 1738, 2393, 2752
AflIII	ACRYGT	1 cut(s)	1149
AjiI	CACGTC	1 cut(s)	220
AjnI	CCWGG	5 cut(s)	235, 1382, 2428, 3058, 3088
AjuI	GAANNNNNNNTTGG	4 cut(s)	2329, 2361, 2396, 2428
AleI	CACNNNNGTG	2 cut(s)	1125, 2966
Alw21I	GWGCWC	3 cut(s)	100, 491, 2599
Alw26I	GTCTC	6 cut(s)	226, 672, 1264, 1412, 1981, 2102
Alw44I	GTGCAC	1 cut(s)	96
AlwI	GGATC	7 cut(s)	36, 862, 1368, 2287, 2404, 2427, 2440
AlwNI	CAGNNNCTG	3 cut(s)	647, 670, 1491
Ama87I	CYCGRG	2 cut(s)	1222, 2448
AoxI	GGCC	7 cut(s)	43, 72, 394, 1192, 1309, 1657, 2198
ApaI	GGGCCC	1 cut(s)	47
ApaLI	GTGCAC	1 cut(s)	96
ApeKI	GCWGC	9 cut(s)	968, 1448, 1586, 1952, 2082, 2085, 2313, 2714, 2846
ApoI	RAATTY	4 cut(s)	134, 627, 1097, 1877
Asp700I	GAANNNNTTC	3 cut(s)	537, 874, 2580
Asp718I	GGTACC	1 cut(s)	1378
AspLEI	GCGC	2 cut(s)	9, 2355
AspS9I	GGNCC	9 cut(s)	43, 44, 661, 1219, 1238, 1277, 1657, 1702, 2198
AsuC2I	CCSGG	1 cut(s)	3222
AsuHPI	GGTGA	6 cut(s)	518, 803, 845, 1157, 1562, 2451
AvaI	CYCGRG	2 cut(s)	1222, 2448
AvaII	GGWCC	5 cut(s)	661, 1219, 1238, 1277, 1702
BaeGI	GKGCMC	3 cut(s)	47, 100, 1575
BalI	TGGCCA	2 cut(s)	1194, 1311
BamHI	GGATCC	1 cut(s)	2432
BanI	GGYRCC	2 cut(s)	1378, 2113
BanII	GRGCYC	3 cut(s)	47, 491, 2599
BarI	GAAGNNNNNNTAC	2 cut(s)	418, 450
BauI	CACGAG	1 cut(s)	2487
BbrPI	CACGTG	1 cut(s)	392
BbsI	GAAGAC	1 cut(s)	1955
Bbv12I	GWGCWC	3 cut(s)	100, 491, 2599
BbvI	GCAGC	9 cut(s)	955, 1460, 1573, 1964, 2094, 2097, 2325, 2726, 2858
BceAI	ACGGC	2 cut(s)	1716, 2340
BcgI	CGANNNNNNTGC	2 cut(s)	639, 673
BciT130I	CCWGG	5 cut(s)	237, 1384, 2430, 3060, 3090
BciVI	GTATCC	2 cut(s)	276, 1858
BclI	TGATCA	1 cut(s)	1987
BcnI	CCSGG	1 cut(s)	3222
BcoDI	GTCTC	6 cut(s)	226, 672, 1264, 1412, 1981, 2102
BfaI	CTAG	5 cut(s)	879, 1349, 1697, 2478, 2991
BfmI	CTRYAG	3 cut(s)	924, 1446, 2844
BfoI	RGCGCY	1 cut(s)	2356
BfuAI	ACCTGC	1 cut(s)	707
BfuI	GTATCC	2 cut(s)	276, 1858
BglII	AGATCT	2 cut(s)	2608, 3103
BmcAI	AGTACT	2 cut(s)	930, 1171
Bme1390I	CCNGG	6 cut(s)	237, 1384, 2430, 3060, 3090, 3222
Bme18I	GGWCC	5 cut(s)	661, 1219, 1238, 1277, 1702
BmeT110I	CYCGRG	2 cut(s)	1222, 2448
BmgBI	CACGTC	1 cut(s)	220
BmgT120I	GGNCC	9 cut(s)	43, 44, 661, 1219, 1238, 1277, 1657, 1702, 2198
BmiI	GGNNCC	9 cut(s)	45, 534, 1220, 1221, 1380, 1703, 1704, 2115, 2434
BmrFI	CCNGG	6 cut(s)	237, 1384, 2430, 3060, 3090, 3222
BmrI	ACTGGG	1 cut(s)	2814
BmsI	GCATC	7 cut(s)	715, 784, 1033, 1063, 1066, 2453, 2536
BmuI	ACTGGG	1 cut(s)	2814
BpiI	GAAGAC	1 cut(s)	1955
BplI	GAGNNNNNCTC	2 cut(s)	253, 285
BpmI	CTGGAG	2 cut(s)	208, 3111
Bpu10I	CCTNAGC	1 cut(s)	2598
BpuEI	CTTGAG	4 cut(s)	92, 1692, 2084, 3092
BpuMI	CCSGG	1 cut(s)	3222
Bsa29I	ATCGAT	1 cut(s)	1994
BsaAI	YACGTR	2 cut(s)	392, 860
BsaI	GGTCTC	2 cut(s)	1412, 1981
BsaJI	CCNNGG	7 cut(s)	1189, 1195, 1280, 1382, 1383, 2109, 3231
BsaWI	WCCGGW	3 cut(s)	1142, 1375, 3205
BsaXI	ACNNNNNCTCC	4 cut(s)	1379, 1409, 2317, 2347
Bsc4I	CCNNNNNNNGG	7 cut(s)	261, 575, 1247, 1688, 1738, 2393, 2752
Bse1I	ACTGG	4 cut(s)	373, 664, 2307, 2820
BseBI	CCWGG	5 cut(s)	237, 1384, 2430, 3060, 3090
BseCI	ATCGAT	1 cut(s)	1994
BseDI	CCNNGG	7 cut(s)	1189, 1195, 1280, 1382, 1383, 2109, 3231
BseLI	CCNNNNNNNGG	7 cut(s)	261, 575, 1247, 1688, 1738, 2393, 2752
BseMII	CTCAG	5 cut(s)	198, 2340, 2430, 2561, 2612
BseNI	ACTGG	4 cut(s)	373, 664, 2307, 2820
BseRI	GAGGAG	3 cut(s)	209, 886, 3108
BseSI	GKGCMC	3 cut(s)	47, 100, 1575
BseXI	GCAGC	9 cut(s)	955, 1460, 1573, 1964, 2094, 2097, 2325, 2726, 2858
BseYI	CCCAGC	1 cut(s)	2506
BsgI	GTGCAG	2 cut(s)	735, 2379
BshFI	GGCC	7 cut(s)	45, 74, 396, 1194, 1311, 1659, 2200
BshNI	GGYRCC	2 cut(s)	1378, 2113
BshVI	ATCGAT	1 cut(s)	1994
BsiHKAI	GWGCWC	3 cut(s)	100, 491, 2599
BsiHKCI	CYCGRG	2 cut(s)	1222, 2448
BsiSI	CCGG	4 cut(s)	1143, 1376, 3206, 3221
BslFI	GGGAC	5 cut(s)	71, 1205, 1381, 1688, 2808
BslI	CCNNNNNNNGG	7 cut(s)	261, 575, 1247, 1688, 1738, 2393, 2752
BsmAI	GTCTC	6 cut(s)	226, 672, 1264, 1412, 1981, 2102
BsmBI	CGTCTC	1 cut(s)	226
BsmFI	GGGAC	5 cut(s)	71, 1205, 1381, 1688, 2808
BsnI	GGCC	7 cut(s)	45, 74, 396, 1194, 1311, 1659, 2200
Bso31I	GGTCTC	2 cut(s)	1412, 1981
BsoBI	CYCGRG	2 cut(s)	1222, 2448
Bsp120I	GGGCCC	1 cut(s)	43
Bsp1286I	GDGCHC	5 cut(s)	47, 100, 491, 1575, 2599
Bsp1407I	TGTACA	1 cut(s)	1152
Bsp19I	CCATGG	4 cut(s)	1195, 1280, 2109, 3231
BspACI	CCGC	8 cut(s)	26, 41, 1110, 1411, 1623, 3118, 3133, 3167
BspANI	GGCC	7 cut(s)	45, 74, 396, 1194, 1311, 1659, 2200
BspCNI	CTCAG	5 cut(s)	197, 2341, 2429, 2562, 2611
BspDI	ATCGAT	1 cut(s)	1994
BspHI	TCATGA	2 cut(s)	781, 2830
BspLI	GGNNCC	9 cut(s)	45, 534, 1220, 1221, 1380, 1703, 1704, 2115, 2434
BspMAI	CTGCAG	2 cut(s)	1450, 2848
BspMI	ACCTGC	1 cut(s)	707
BspPI	GGATC	7 cut(s)	36, 862, 1368, 2287, 2404, 2427, 2440
BspT107I	GGYRCC	2 cut(s)	1378, 2113
BspTNI	GGTCTC	2 cut(s)	1412, 1981
BsrGI	TGTACA	1 cut(s)	1152
BsrI	ACTGG	4 cut(s)	373, 664, 2307, 2820
BssECI	CCNNGG	7 cut(s)	1189, 1195, 1280, 1382, 1383, 2109, 3231
BssSI	CACGAG	1 cut(s)	2487
BssT1I	CCWWGG	5 cut(s)	1189, 1195, 1280, 2109, 3231
Bst2BI	CACGAG	1 cut(s)	2487
Bst2UI	CCWGG	5 cut(s)	237, 1384, 2430, 3060, 3090
Bst4CI	ACNGT	9 cut(s)	102, 1417, 1493, 1639, 2153, 2567, 2664, 2683, 3159
Bst6I	CTCTTC	4 cut(s)	1005, 1748, 2194, 2209
BstAPI	GCANNNNNTGC	3 cut(s)	1173, 2319, 2366
BstAUI	TGTACA	1 cut(s)	1152
BstBAI	YACGTR	2 cut(s)	392, 860
BstC8I	GCNNGC	6 cut(s)	43, 72, 1108, 1309, 1621, 2202
BstDEI	CTNAG	7 cut(s)	184, 212, 2165, 2349, 2416, 2570, 2598
BstDSI	CCRYGG	4 cut(s)	1195, 1280, 2109, 3231
BstEII	GGTNACC	1 cut(s)	2769
BstENI	CCTNNNNNAGG	1 cut(s)	259
BstH2I	RGCGCY	1 cut(s)	2356
BstHHI	GCGC	2 cut(s)	9, 2355
BstMAI	GTCTC	6 cut(s)	226, 672, 1264, 1412, 1981, 2102
BstNI	CCWGG	5 cut(s)	237, 1384, 2430, 3060, 3090
BstNSI	RCATGY	4 cut(s)	654, 968, 1110, 1153
BstPI	GGTNACC	1 cut(s)	2769
BstSCI	CCNGG	6 cut(s)	235, 1382, 2428, 3058, 3088, 3220
BstSFI	CTRYAG	3 cut(s)	924, 1446, 2844
BstSLI	GKGCMC	3 cut(s)	47, 100, 1575
BstV1I	GCAGC	9 cut(s)	955, 1460, 1573, 1964, 2094, 2097, 2325, 2726, 2858
BstV2I	GAAGAC	1 cut(s)	1955
BstX2I	RGATCY	4 cut(s)	2279, 2432, 2608, 3103
BstYI	RGATCY	4 cut(s)	2279, 2432, 2608, 3103
Bsu15I	ATCGAT	1 cut(s)	1994
BsuI	GTATCC	2 cut(s)	276, 1858
BsuRI	GGCC	7 cut(s)	45, 74, 396, 1194, 1311, 1659, 2200
BsuTUI	ATCGAT	1 cut(s)	1994
BtgI	CCRYGG	4 cut(s)	1195, 1280, 2109, 3231
BtrI	CACGTC	1 cut(s)	220
BtsI	GCAGTG	4 cut(s)	78, 645, 1443, 1458
BtsIMutI	CAGTG	8 cut(s)	78, 645, 1413, 1443, 1458, 2961, 3145, 3164
BveI	ACCTGC	1 cut(s)	707
Cac8I	GCNNGC	6 cut(s)	43, 72, 1108, 1309, 1621, 2202
CaiI	CAGNNNCTG	3 cut(s)	647, 670, 1491
CciI	TCATGA	2 cut(s)	781, 2830
CfoI	GCGC	2 cut(s)	9, 2355
Cfr13I	GGNCC	9 cut(s)	43, 44, 661, 1219, 1238, 1277, 1657, 1702, 2198
ClaI	ATCGAT	1 cut(s)	1994
CseI	GACGC	1 cut(s)	126
CsiI	ACCWGGT	1 cut(s)	235
Csp6I	GTAC	6 cut(s)	181, 929, 1153, 1170, 1379, 3196
CspCI	CAANNNNNGTGG	4 cut(s)	1564, 1599, 2520, 2555
CviQI	GTAC	6 cut(s)	181, 929, 1153, 1170, 1379, 3196
DdeI	CTNAG	7 cut(s)	184, 212, 2165, 2349, 2416, 2570, 2598
DraI	TTTAAA	1 cut(s)	2029
DraIII	CACNNNGTG	1 cut(s)	2489
DrdI	GACNNNNNNGTC	1 cut(s)	1916
DseDI	GACNNNNNNGTC	1 cut(s)	1916
EaeI	YGGCCR	2 cut(s)	1192, 1309
Eam1104I	CTCTTC	4 cut(s)	1005, 1748, 2194, 2209
EarI	CTCTTC	4 cut(s)	1005, 1748, 2194, 2209
Ecl136II	GAGCTC	2 cut(s)	489, 2597
Eco130I	CCWWGG	5 cut(s)	1189, 1195, 1280, 2109, 3231
Eco24I	GRGCYC	3 cut(s)	47, 491, 2599
Eco31I	GGTCTC	2 cut(s)	1412, 1981
Eco47I	GGWCC	5 cut(s)	661, 1219, 1238, 1277, 1702
Eco53kI	GAGCTC	2 cut(s)	489, 2597
Eco57I	CTGAAG	7 cut(s)	375, 462, 624, 1836, 2985, 3039, 3106
Eco72I	CACGTG	1 cut(s)	392
Eco88I	CYCGRG	2 cut(s)	1222, 2448
Eco91I	GGTNACC	1 cut(s)	2769
EcoICRI	GAGCTC	2 cut(s)	489, 2597
EcoNI	CCTNNNNNAGG	1 cut(s)	259
EcoO109I	RGGNCCY	2 cut(s)	1219, 1702
EcoO65I	GGTNACC	1 cut(s)	2769
EcoRII	CCWGG	5 cut(s)	235, 1382, 2428, 3058, 3088
EcoT14I	CCWWGG	5 cut(s)	1189, 1195, 1280, 2109, 3231
EcoT38I	GRGCYC	3 cut(s)	47, 491, 2599
ErhI	CCWWGG	5 cut(s)	1189, 1195, 1280, 2109, 3231
Esp3I	CGTCTC	1 cut(s)	226
FaqI	GGGAC	5 cut(s)	71, 1205, 1381, 1688, 2808
FauI	CCCGC	2 cut(s)	34, 1103
FbaI	TGATCA	1 cut(s)	1987
FblI	GTMKAC	1 cut(s)	1537
FriOI	GRGCYC	3 cut(s)	47, 491, 2599
FspBI	CTAG	5 cut(s)	879, 1349, 1697, 2478, 2991
FspI	TGCGCA	1 cut(s)	8
GlaI	GCGC	2 cut(s)	8, 2354
GsaI	CCCAGC	1 cut(s)	2510
GsuI	CTGGAG	2 cut(s)	208, 3111
HaeII	RGCGCY	1 cut(s)	2356
HaeIII	GGCC	7 cut(s)	45, 74, 396, 1194, 1311, 1659, 2200
HapII	CCGG	4 cut(s)	1143, 1376, 3206, 3221
HgaI	GACGC	1 cut(s)	126
HhaI	GCGC	2 cut(s)	9, 2355
Hin6I	GCGC	2 cut(s)	7, 2353
HinP1I	GCGC	2 cut(s)	7, 2353
HincII	GTYRAC	2 cut(s)	1538, 1694
HindII	GTYRAC	2 cut(s)	1538, 1694
HindIII	AAGCTT	2 cut(s)	1505, 1899
HpaII	CCGG	4 cut(s)	1143, 1376, 3206, 3221
HphI	GGTGA	6 cut(s)	518, 803, 845, 1157, 1562, 2451
HpyCH4III	ACNGT	9 cut(s)	102, 1417, 1493, 1639, 2153, 2567, 2664, 2683, 3159
HpyCH4IV	ACGT	3 cut(s)	219, 391, 859
HpyF3I	CTNAG	7 cut(s)	184, 212, 2165, 2349, 2416, 2570, 2598
HpySE526I	ACGT	3 cut(s)	219, 391, 859
HspAI	GCGC	2 cut(s)	7, 2353
KflI	GGGWCCC	2 cut(s)	1219, 1702
KpnI	GGTACC	1 cut(s)	1382
Ksp22I	TGATCA	1 cut(s)	1987
LmnI	GCTCC	1 cut(s)	1191
Lsp1109I	GCAGC	9 cut(s)	955, 1460, 1573, 1964, 2094, 2097, 2325, 2726, 2858
LweI	GCATC	7 cut(s)	715, 784, 1033, 1063, 1066, 2453, 2536
MabI	ACCWGGT	1 cut(s)	235
MaeI	CTAG	5 cut(s)	879, 1349, 1697, 2478, 2991
MaeII	ACGT	3 cut(s)	219, 391, 859
MfeI	CAATTG	4 cut(s)	1313, 2044, 2317, 2747
MflI	RGATCY	4 cut(s)	2279, 2432, 2608, 3103
MhlI	GDGCHC	5 cut(s)	47, 100, 491, 1575, 2599
MlsI	TGGCCA	2 cut(s)	1194, 1311
MluNI	TGGCCA	2 cut(s)	1194, 1311
MlyI	GAGTC	4 cut(s)	887, 1403, 2807, 3105
MmeI	TCCRAC	1 cut(s)	847
Mox20I	TGGCCA	2 cut(s)	1194, 1311
MroXI	GAANNNNTTC	3 cut(s)	537, 874, 2580
MscI	TGGCCA	2 cut(s)	1194, 1311
MslI	CAYNNNNRTG	3 cut(s)	786, 1125, 2966
Msp20I	TGGCCA	2 cut(s)	1194, 1311
MspA1I	CMGCKG	1 cut(s)	1292
MspI	CCGG	4 cut(s)	1143, 1376, 3206, 3221
MspR9I	CCNGG	6 cut(s)	237, 1384, 2430, 3060, 3090, 3222
MunI	CAATTG	4 cut(s)	1313, 2044, 2317, 2747
MvaI	CCWGG	5 cut(s)	237, 1384, 2430, 3060, 3090
NciI	CCSGG	1 cut(s)	3222
NcoI	CCATGG	4 cut(s)	1195, 1280, 2109, 3231
NlaIV	GGNNCC	9 cut(s)	45, 534, 1220, 1221, 1380, 1703, 1704, 2115, 2434
NmuCI	GTSAC	5 cut(s)	1145, 1297, 1550, 2144, 2968
NsbI	TGCGCA	1 cut(s)	8
NspI	RCATGY	4 cut(s)	654, 968, 1110, 1153
OliI	CACNNNNGTG	2 cut(s)	1125, 2966
PaeI	GCATGC	1 cut(s)	1110
PagI	TCATGA	2 cut(s)	781, 2830
PaqCI	CACCTGC	1 cut(s)	707
PasI	CCCWGGG	1 cut(s)	1383
PciI	ACATGT	1 cut(s)	1149
PdmI	GAANNNNTTC	3 cut(s)	537, 874, 2580
PfeI	GAWTC	6 cut(s)	164, 419, 917, 1252, 2227, 2235
PflFI	GACNNNGTC	1 cut(s)	1629
PflMI	CCANNNNNTGG	2 cut(s)	575, 2393
PfoI	TCCNGGA	1 cut(s)	2428
PleI	GAGTC	4 cut(s)	887, 1402, 2807, 3104
PmaCI	CACGTG	1 cut(s)	392
PmlI	CACGTG	1 cut(s)	392
PpsI	GAGTC	4 cut(s)	887, 1402, 2807, 3104
Ppu21I	YACGTR	2 cut(s)	392, 860
PpuMI	RGGWCCY	2 cut(s)	1219, 1702
PscI	ACATGT	1 cut(s)	1149
PsiI	TTATAA	1 cut(s)	2052
Psp124BI	GAGCTC	2 cut(s)	491, 2599
Psp5II	RGGWCCY	2 cut(s)	1219, 1702
Psp6I	CCWGG	5 cut(s)	235, 1382, 2428, 3058, 3088
PspCI	CACGTG	1 cut(s)	392
PspEI	GGTNACC	1 cut(s)	2769
PspFI	CCCAGC	1 cut(s)	2506
PspGI	CCWGG	5 cut(s)	235, 1382, 2428, 3058, 3088
PspN4I	GGNNCC	9 cut(s)	45, 534, 1220, 1221, 1380, 1703, 1704, 2115, 2434
PspOMI	GGGCCC	1 cut(s)	43
PspPI	GGNCC	9 cut(s)	43, 44, 661, 1219, 1238, 1277, 1657, 1702, 2198
PspPPI	RGGWCCY	2 cut(s)	1219, 1702
PsrI	GAACNNNNNNTAC	2 cut(s)	921, 953
PstI	CTGCAG	2 cut(s)	1450, 2848
PstNI	CAGNNNCTG	3 cut(s)	647, 670, 1491
PsuI	RGATCY	4 cut(s)	2279, 2432, 2608, 3103
PsyI	GACNNNGTC	1 cut(s)	1629
PvuII	CAGCTG	1 cut(s)	1292
RsaI	GTAC	6 cut(s)	182, 930, 1154, 1171, 1380, 3197
RsaNI	GTAC	6 cut(s)	181, 929, 1153, 1170, 1379, 3196
RseI	CAYNNNNRTG	3 cut(s)	786, 1125, 2966
SacI	GAGCTC	2 cut(s)	491, 2599
SalI	GTCGAC	1 cut(s)	1536
Sau96I	GGNCC	9 cut(s)	43, 44, 661, 1219, 1238, 1277, 1657, 1702, 2198
ScaI	AGTACT	2 cut(s)	930, 1171
SchI	GAGTC	4 cut(s)	887, 1403, 2807, 3105
ScrFI	CCNGG	6 cut(s)	237, 1384, 2430, 3060, 3090, 3222
SduI	GDGCHC	5 cut(s)	47, 100, 491, 1575, 2599
SexAI	ACCWGGT	1 cut(s)	235
SfaNI	GCATC	7 cut(s)	715, 784, 1033, 1063, 1066, 2453, 2536
SfcI	CTRYAG	3 cut(s)	924, 1446, 2844
SinI	GGWCC	5 cut(s)	661, 1219, 1238, 1277, 1702
SmiMI	CAYNNNNRTG	3 cut(s)	786, 1125, 2966
SmlI	CTYRAG	4 cut(s)	107, 1707, 2099, 3107
SmoI	CTYRAG	4 cut(s)	107, 1707, 2099, 3107
SphI	GCATGC	1 cut(s)	1110
SsiI	CCGC	8 cut(s)	26, 41, 1110, 1411, 1623, 3118, 3133, 3167
SspMI	CTAG	5 cut(s)	879, 1349, 1697, 2478, 2991
SstI	GAGCTC	2 cut(s)	491, 2599
StyD4I	CCNGG	6 cut(s)	235, 1382, 2428, 3058, 3088, 3220
StyI	CCWWGG	5 cut(s)	1189, 1195, 1280, 2109, 3231
TaaI	ACNGT	9 cut(s)	102, 1417, 1493, 1639, 2153, 2567, 2664, 2683, 3159
TaiI	ACGT	3 cut(s)	222, 394, 862
TaqI	TCGA	8 cut(s)	51, 271, 1363, 1537, 1808, 1849, 1994, 3127
TaqII	GACCGA	2 cut(s)	306, 649
TatI	WGTACW	4 cut(s)	928, 1152, 1169, 3195
TauI	GCSGC	1 cut(s)	3170
TfiI	GAWTC	6 cut(s)	164, 419, 917, 1252, 2227, 2235
TscAI	CASTG	8 cut(s)	85, 652, 1420, 1450, 1458, 2968, 3145, 3164
TseFI	GTSAC	5 cut(s)	1145, 1297, 1550, 2144, 2968
TseI	GCWGC	9 cut(s)	968, 1448, 1586, 1952, 2082, 2085, 2313, 2714, 2846
Tsp45I	GTSAC	5 cut(s)	1145, 1297, 1550, 2144, 2968
TspRI	CASTG	8 cut(s)	85, 652, 1420, 1450, 1458, 2968, 3145, 3164
Tth111I	GACNNNGTC	1 cut(s)	1629
Van91I	CCANNNNNTGG	2 cut(s)	575, 2393
VneI	GTGCAC	1 cut(s)	96
VpaK11BI	GGWCC	5 cut(s)	661, 1219, 1238, 1277, 1702
XagI	CCTNNNNNAGG	1 cut(s)	259
XapI	RAATTY	4 cut(s)	134, 627, 1097, 1877
XceI	RCATGY	4 cut(s)	654, 968, 1110, 1153
XmiI	GTMKAC	1 cut(s)	1537
XmnI	GAANNNNTTC	3 cut(s)	537, 874, 2580
XspI	CTAG	5 cut(s)	879, 1349, 1697, 2478, 2991
ZrmI	AGTACT	2 cut(s)	930, 1171

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.