Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0003738

Physical Location & Seq

Genomic Coordinates Forward (+)

22552664 .. 22560052

Gene Structure 7389 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g24410.8

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3237 bp

ATGCTGCGTATGGACAGGATGACTGCGGATCAGGCAAAGGCGGGCCCACTCGAAAGGGACATTGACTTGGCCATCACTGCTCTGAAAAAGGGGGCACAATTGCTCAAGTATGGACGCAGAGGGAAACCCAAATTTTGTCCTTTCAGGCTGTCCAATGATGAATCTGTTCTAATATGGTACTCAGGGAAAGAGGAGAAATATGTTAAACTAAGCCAAGTCTCGAGAATTGTACCTGGTCAGCGCACTCAAACCTTTCAGAGGTATCCTCGGCCTGAAAAGGAATATCAGTCATTTTCTCTTATATATAATGATAGGTCTTTGGATTTGATATGCAAGGATAAAGATGAAGCTGAAGTTTGGTTTACTGGTCTAAAAGCATTAATATCACGTGGCCTTCACCAGAAGCGGAGAGCAGAATCAAGAAGTGGAGTTACATCTGAGGCAAATAGTCCTAGATCACACACCCAACAAATTTCTCCATTGAGCTCTCCATTTTGTAGTGGTGATAGTTCGCAGAAGGATGGCGTGGAACCGTTTCATCTTCATGCTGCATTTGAAAGCCCTCCTAAAATTGGTTTAGAGAAGGTTTTATCGGATGTCATGTTGTATGCCCTGCCTCCAAAAATTCCCTTCCCTTCAGATGCTTCTTGTGGCTCAGTCCCCTCTTCGGGAGGCTCAGATGGAATGAACGGACGAACAAGGGGTGCAGGTGTGGATGCATTTAGAGTAAGTTTATCAAGTGCTGTTAGCTCATCAAGTCAAGGCTCTGGTCATGATGAAGGTGATGCTTTAGCCTTAGGGGATGTTTTCATTTGGGGGGAAGGCGTTGGTGATTGCTTTCTGGGTGGAGGATCACATAGAGTTGGAAGTTCTTCTGGTTGTAAGATGGATTCTTTTGTGCCTAAAGTCTTGGAATCTGCCGTAGTGCTTGATGTCCAAAGCATAGCTTGTGGTGGACGGCATGCTGCTTTTGTAACCAAGCAAGGGGAGGTATTTTCTTGGGGTGAAGAATTAGGAGGCAGACTGGGACACGGTGTGGATGCTGATGTTTCACATCCAAAACTCATAGATGCTCTGAAAAACATGAATATTGAATTATTAGCATGTGGGGAATACCATTCTTGTGCTGTCACACTTTCCGGTGACATGTACACATGGGGTGGCAGTAATTCCAATTTTGCACTCCTTGGTCATGGGTCTCAATCAAGTCAATGGGTCCCAAAAAAGCTAAATGGACCTTTAGAGGGAATTCATGTCTCATCGGTTTCATGTGGACCATGGCACACAGCTGTTGTGACCTCCGCTGGGCAATTGTTTACTTTTGGCGATGGCACATTTGGTGTTCTAGGTCATGGGGATCGAAAGAGCACTTCGGTGCCCAGGGAGGTTGATTCCCTTAAAGGTCTCCGCACTGTGCGAGTAGCTTGTGGTGTTTGGCACACTGCAGCAGTAATTGAAGTTATGGTGGGGTCATCAAGTTCCAGCAATTGCTCTTCTGGAAAGCTTTTTACATGGGGAGATGGAGATAAGGGTCGACTTGGACATGGTGATAAGGAAGCAAGACTTGTGCCCACTTGTGTTGCTGCTTTGGTTGAACCCAACTTCTGTAAAGTTGCCTGCGGACAAAGCATGACTGTTGCACTTACAACTACGGGCCATGTTTACACTATGGGAAGCCCTGTTTTTGGTCAATTAGGGGTCCCTAAAGCTGATGGGAAACTTCCAAGACGTGTAGAGGGAAAGCTAATGAAGAGTTTTGTTGAAGAAATTGCTTGTGGTGCTAATCATGTTGCAGTTTTAACTTCAAGAACTGAAGTTTACACTTGGGGAAAGGGTGCAAATGGTCGATTAGGTCTTGGGGATACAGAAGACAAATTTTCACCAACATTGGTTGAAGCTTTAAAAGACAAACAAGTCAAGAGTATTGTCTGTGGCGCTAACTTTACTGCTGCTATCTGTCTTCACAAATGGGTCTCTGGTATTGATCGATCAATGTGTTCTGGCTGTCGCCTTCCATTTAATTTTAAAAGAAAAAGACACAATTGTTATAATTGTGGACTTGTGTTATGCCATTCATGCAGCAGCAACAAGTGTCTCAAGGCTTCCATGGCACCAAACCCAAACAAACCTTATCGTGTCTGTGATAACTGTTTTGCAAGACTTAGGAAGGCCACTGAAACCAACTCTTCATCTGGGTCTGCTATCAGTAGAAGGGGAAGTATGAATCAGGGATTCAACGAACTGATTGAAAACAATGTTAAAGAAATGGAAAATGGATCTTCCAAAAGCAACAAGAAAATGGATTTCAGCAGCAATTGTGTTACTCCCATTGCAAATGGAGTTTATGAGCGTCGCGCACTCAATGGGACTAAATCTTTCAACCCTGGATCATCCAAAAAGTTTTTCTCTGCTTCGCTTCCTGGATCCAGAATTGCATCTCGAGCAACATCACCAACATCGAGACGATCTAGTCCACCTCGTTCCACAACACCAACCCCAGCTCTTTCAGGGCTTACTTTTCCAAAAGTTGGGGATGATGCTAAAAGGATAAATGTCAATCTGAGTGAAGAAGTTCTAAAATTAAAAGCTCAGGTTGAAGATCTTACTCGTAAGGCTCAACTTCAAGACATTGAGATGGATAAAACTACCCAACAGTTGAAGGAGGCTCTGAAAGTAGCAGGGGAAGAAACTCTGAAATGCAAAGCAGCGAAGGAAGTAATTAAGTCGCTCACAGCCCAATTAAAGGAAATGGCTGAAAGGTTACCAATTGGAATGGCAAGAAACATGGATACTCGTCCGTTAGACTCTTCCAGTCCCACTCATGAAGTTTCTACTACAGCCGTTGAGAAAACAAGCAGTCCATTGAATTTCCATGAACCTTATTCAGTTGTATCGGACAGTGTGATGATTTCTGATGTGCCACGGGCCACTTCGAATCACACAGAAGTGACACATTCAGAAGCAACAGCTAGGCATAAAAACAGAGGAACAAAGACTGAAATAGCACAAGGGGATGAGTGGGTTGAGCAAGATGAGCCTGGTGTATATATTACCCTTGTTTCCTTGCCTGGAGGTGTCAAAGATCTCAAGCGTGTTCGTTTCAGTCGAAAGCGGTTCAGCGAGAAACAAGCAGAACAGTGGTGGGCGGCAAATAGGGGTAGAGTATATCAGCAGTACAATGTGCCGGTGGTTGAAAAATCCAGTATTCCGATGGGGAGAGAAGGGTTAGCTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRX BRX_N BRX_assoc FYVE PH_12 RCC1

Protein Analysis

1079

Amino Acids

116.65

Weight (kDa)

9.08

Isoelectric Point (pI)

42.88

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12350 AT5G19420 AT5G19420 AT5G19420
fragaria_vesca	FvH4_7g02960 FvH4_7g02960 FvH4_7g02960 FvH4_7g02960
malus_domestica	MD02G1288400.v1.1 MD07G1038100.v1.1 MD07G1038400.v1.1 MD13G1037300.v1.1
prunus_persica	Prupe.2G034600_v2.0.a1 Prupe.2G034600_v2.0.a1 Prupe.2G034600_v2.0.a1
pyrus_communis	pycom02g24410 pycom05g01140 pycom07g02640 pycom07g02670 pycom16g06500
rosa_chinensis	RchiOBHm_Chr1g0323931
rosa_laevigata	RLG00000030242
rosa_multiflora	Rmu_co8261369.1_g000001 Rmu_sc0001449.1_g000001 Rmu_sc0011010.1_g000012 Rmu_sc0012343.1_g000006 Rmu_sc0012343.1_g000007
rosa_roxburghii	Rroxscaffold_4G00326110
rosa_rugosa	Rorug01G0040600
rosa_samantha	Rh1AG058500 Rh1BG049600 Rh1CG060400 Rh1DG064400
rosa_wichuraiana	Rw0G011380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2049
AarI	CACCTGC	1 cut(s)	698
AasI	GACNNNNNNGTC	2 cut(s)	1913, 2469
Acc36I	ACCTGC	1 cut(s)	698
AccB1I	GGYRCC	2 cut(s)	1375, 2110
AccB7I	CCANNNNNTGG	1 cut(s)	2528
AccI	GTMKAC	1 cut(s)	1534
AccII	CGCG	1 cut(s)	2355
AciI	CCGC	8 cut(s)	26, 41, 406, 1302, 1408, 1620, 3112, 3146
AclWI	GGATC	7 cut(s)	36, 859, 1365, 2284, 2395, 2418, 2431
AcoI	YGGCCR	1 cut(s)	69
AcsI	RAATTY	6 cut(s)	131, 471, 624, 1248, 1874, 2866
AcuI	CTGAAG	3 cut(s)	372, 621, 1833
AcvI	CACGTG	1 cut(s)	389
AdeI	CACNNNGTG	1 cut(s)	1036
AfaI	GTAC	4 cut(s)	179, 231, 1151, 3176
AflIII	ACRYGT	2 cut(s)	1146, 1729
AjiI	CACGTC	1 cut(s)	1730
AjnI	CCWGG	6 cut(s)	232, 1379, 2383, 2419, 3037, 3067
AjuI	GAANNNNNNNTTGG	2 cut(s)	2387, 2419
AleI	CACNNNNGTG	2 cut(s)	1373, 2945
Alw21I	GWGCWC	2 cut(s)	488, 1370
Alw26I	GTCTC	7 cut(s)	223, 1203, 1261, 1409, 1978, 2099, 2455
AlwI	GGATC	7 cut(s)	36, 859, 1365, 2284, 2395, 2418, 2431
Ama87I	CYCGRG	2 cut(s)	220, 2439
AoxI	GGCC	7 cut(s)	43, 69, 269, 391, 1654, 2169, 2925
ApaI	GGGCCC	1 cut(s)	47
ApoI	RAATTY	6 cut(s)	131, 471, 624, 1248, 1874, 2866
AseI	ATTAAT	1 cut(s)	380
Asp700I	GAANNNNTTC	4 cut(s)	165, 534, 871, 2571
AspLEI	GCGC	3 cut(s)	243, 1937, 2357
AspS9I	GGNCC	8 cut(s)	43, 44, 1216, 1235, 1274, 1654, 1699, 2925
AsuHPI	GGTGA	9 cut(s)	389, 515, 794, 842, 1016, 1154, 1559, 1872, 2442
AsuII	TTCGAA	1 cut(s)	2933
AvaI	CYCGRG	2 cut(s)	220, 2439
AvaII	GGWCC	4 cut(s)	1216, 1235, 1274, 1699
AxyI	CCTNAGG	1 cut(s)	796
BaeGI	GKGCMC	4 cut(s)	47, 97, 1380, 1572
BalI	TGGCCA	1 cut(s)	71
BamHI	GGATCC	1 cut(s)	2423
BanI	GGYRCC	2 cut(s)	1375, 2110
BanII	GRGCYC	2 cut(s)	47, 488
BarI	GAAGNNNNNNTAC	2 cut(s)	415, 447
BbrPI	CACGTG	1 cut(s)	389
BbsI	GAAGAC	2 cut(s)	1875, 1952
Bbv12I	GWGCWC	2 cut(s)	488, 1370
BbvI	GCAGC	9 cut(s)	535, 952, 1457, 1570, 1936, 2091, 2094, 2322, 2717
BccI	CCATC	9 cut(s)	80, 515, 674, 880, 1322, 1514, 1706, 2629, 3205
BceAI	ACGGC	3 cut(s)	905, 974, 2825
BciT130I	CCWGG	6 cut(s)	234, 1381, 2385, 2421, 3039, 3069
BciVI	GTATCC	3 cut(s)	273, 1855, 2782
BcoDI	GTCTC	7 cut(s)	223, 1203, 1261, 1409, 1978, 2099, 2455
BfaI	CTAG	4 cut(s)	453, 1346, 2469, 2970
BfmI	CTRYAG	2 cut(s)	1443, 2835
BfoI	RGCGCY	1 cut(s)	1938
BfuAI	ACCTGC	1 cut(s)	698
BfuI	GTATCC	3 cut(s)	273, 1855, 2782
BglII	AGATCT	2 cut(s)	2599, 3082
Bme1390I	CCNGG	6 cut(s)	234, 1381, 2385, 2421, 3039, 3069
Bme18I	GGWCC	4 cut(s)	1216, 1235, 1274, 1699
BmeT110I	CYCGRG	2 cut(s)	220, 2439
BmgBI	CACGTC	1 cut(s)	1730
BmgT120I	GGNCC	8 cut(s)	43, 44, 1216, 1235, 1274, 1654, 1699, 2925
BmiI	GGNNCC	9 cut(s)	45, 531, 1217, 1218, 1377, 1700, 1701, 2112, 2425
BmrFI	CCNGG	6 cut(s)	234, 1381, 2385, 2421, 3039, 3069
BmrI	ACTGGG	1 cut(s)	1034
BmsI	GCATC	7 cut(s)	631, 706, 775, 1030, 1060, 2444, 2527
BmuI	ACTGGG	1 cut(s)	1034
BpiI	GAAGAC	2 cut(s)	1875, 1952
BplI	GAGNNNNNCTC	2 cut(s)	250, 282
BpmI	CTGGAG	1 cut(s)	3090
Bpu10I	CCTNAGC	1 cut(s)	2589
Bpu14I	TTCGAA	1 cut(s)	2933
BpuEI	CTTGAG	3 cut(s)	89, 2081, 3071
Bsa29I	ATCGAT	1 cut(s)	1987
BsaAI	YACGTR	1 cut(s)	389
BsaI	GGTCTC	3 cut(s)	1203, 1409, 1978
BsaJI	CCNNGG	8 cut(s)	266, 1186, 1277, 1379, 1380, 2106, 2383, 2921
BsaWI	WCCGGW	1 cut(s)	1139
Bse118I	RCCGGY	1 cut(s)	3184
Bse1I	ACTGG	4 cut(s)	370, 1029, 2811, 3201
Bse21I	CCTNAGG	1 cut(s)	796
Bse3DI	GCAATG	1 cut(s)	2328
BseBI	CCWGG	6 cut(s)	234, 1381, 2385, 2421, 3039, 3069
BseCI	ATCGAT	1 cut(s)	1987
BseDI	CCNNGG	8 cut(s)	266, 1186, 1277, 1379, 1380, 2106, 2383, 2921
BseMI	GCAATG	1 cut(s)	2328
BseMII	CTCAG	6 cut(s)	195, 429, 669, 690, 2552, 2603
BseNI	ACTGG	4 cut(s)	370, 1029, 2811, 3201
BseRI	GAGGAG	1 cut(s)	206
BseSI	GKGCMC	4 cut(s)	47, 97, 1380, 1572
BseXI	GCAGC	9 cut(s)	535, 952, 1457, 1570, 1936, 2091, 2094, 2322, 2717
BseYI	CCCAGC	2 cut(s)	1304, 2497
BsgI	GTGCAG	1 cut(s)	726
Bsh1236I	CGCG	1 cut(s)	2355
BshFI	GGCC	7 cut(s)	45, 71, 271, 393, 1656, 2171, 2927
BshNI	GGYRCC	2 cut(s)	1375, 2110
BshVI	ATCGAT	1 cut(s)	1987
BsiHKAI	GWGCWC	2 cut(s)	488, 1370
BsiHKCI	CYCGRG	2 cut(s)	220, 2439
BsiSI	CCGG	2 cut(s)	1140, 3185
BslFI	GGGAC	7 cut(s)	71, 644, 1041, 1202, 1685, 2380, 2799
BsmAI	GTCTC	7 cut(s)	223, 1203, 1261, 1409, 1978, 2099, 2455
BsmBI	CGTCTC	1 cut(s)	2455
BsmFI	GGGAC	7 cut(s)	71, 644, 1041, 1202, 1685, 2380, 2799
BsnI	GGCC	7 cut(s)	45, 71, 271, 393, 1656, 2171, 2927
Bso31I	GGTCTC	3 cut(s)	1203, 1409, 1978
BsoBI	CYCGRG	2 cut(s)	220, 2439
Bsp119I	TTCGAA	1 cut(s)	2933
Bsp120I	GGGCCC	1 cut(s)	43
Bsp1286I	GDGCHC	6 cut(s)	47, 97, 488, 1370, 1380, 1572
Bsp1407I	TGTACA	1 cut(s)	1149
Bsp19I	CCATGG	2 cut(s)	1277, 2106
BspACI	CCGC	8 cut(s)	26, 41, 406, 1302, 1408, 1620, 3112, 3146
BspANI	GGCC	7 cut(s)	45, 71, 271, 393, 1656, 2171, 2927
BspCNI	CTCAG	6 cut(s)	194, 430, 668, 689, 2553, 2602
BspDI	ATCGAT	1 cut(s)	1987
BspFNI	CGCG	1 cut(s)	2355
BspHI	TCATGA	2 cut(s)	772, 2821
BspLI	GGNNCC	9 cut(s)	45, 531, 1217, 1218, 1377, 1700, 1701, 2112, 2425
BspMAI	CTGCAG	1 cut(s)	1447
BspMI	ACCTGC	1 cut(s)	698
BspPI	GGATC	7 cut(s)	36, 859, 1365, 2284, 2395, 2418, 2431
BspQI	GCTCTTC	1 cut(s)	1498
BspT104I	TTCGAA	1 cut(s)	2933
BspT107I	GGYRCC	2 cut(s)	1375, 2110
BspTNI	GGTCTC	3 cut(s)	1203, 1409, 1978
BsrDI	GCAATG	1 cut(s)	2328
BsrFI	RCCGGY	1 cut(s)	3184
BsrGI	TGTACA	1 cut(s)	1149
BsrI	ACTGG	4 cut(s)	370, 1029, 2811, 3201
BssAI	RCCGGY	1 cut(s)	3184
BssECI	CCNNGG	8 cut(s)	266, 1186, 1277, 1379, 1380, 2106, 2383, 2921
BssT1I	CCWWGG	3 cut(s)	1186, 1277, 2106
Bst2UI	CCWGG	6 cut(s)	234, 1381, 2385, 2421, 3039, 3069
Bst4CI	ACNGT	8 cut(s)	534, 1034, 1414, 1636, 2150, 2655, 2900, 3138
Bst6I	CTCTTC	5 cut(s)	670, 1498, 1745, 2191, 2812
BstAUI	TGTACA	1 cut(s)	1149
BstBAI	YACGTR	1 cut(s)	389
BstBI	TTCGAA	1 cut(s)	2933
BstC8I	GCNNGC	3 cut(s)	43, 963, 1618
BstDEI	CTNAG	9 cut(s)	181, 209, 438, 655, 676, 796, 2162, 2561, 2589
BstDSI	CCRYGG	3 cut(s)	1277, 2106, 2921
BstEII	GGTNACC	1 cut(s)	2760
BstENI	CCTNNNNNAGG	1 cut(s)	256
BstFNI	CGCG	1 cut(s)	2355
BstH2I	RGCGCY	1 cut(s)	1938
BstHHI	GCGC	3 cut(s)	243, 1937, 2357
BstMAI	GTCTC	7 cut(s)	223, 1203, 1261, 1409, 1978, 2099, 2455
BstMWI	GCNNNNNNNGC	8 cut(s)	32, 77, 1626, 1778, 2076, 2108, 2441, 3034
BstNI	CCWGG	6 cut(s)	234, 1381, 2385, 2421, 3039, 3069
BstNSI	RCATGY	3 cut(s)	965, 1107, 1150
BstPI	GGTNACC	1 cut(s)	2760
BstSCI	CCNGG	6 cut(s)	232, 1379, 2383, 2419, 3037, 3067
BstSFI	CTRYAG	2 cut(s)	1443, 2835
BstSLI	GKGCMC	4 cut(s)	47, 97, 1380, 1572
BstUI	CGCG	1 cut(s)	2355
BstV1I	GCAGC	9 cut(s)	535, 952, 1457, 1570, 1936, 2091, 2094, 2322, 2717
BstV2I	GAAGAC	2 cut(s)	1875, 1952
BstX2I	RGATCY	4 cut(s)	2276, 2423, 2599, 3082
BstYI	RGATCY	4 cut(s)	2276, 2423, 2599, 3082
Bsu15I	ATCGAT	1 cut(s)	1987
Bsu36I	CCTNAGG	1 cut(s)	796
BsuI	GTATCC	3 cut(s)	273, 1855, 2782
BsuRI	GGCC	7 cut(s)	45, 71, 271, 393, 1656, 2171, 2927
BsuTUI	ATCGAT	1 cut(s)	1987
BtgI	CCRYGG	3 cut(s)	1277, 2106, 2921
BtgZI	GCGATG	1 cut(s)	1341
BtrI	CACGTC	1 cut(s)	1730
BtsI	GCAGTG	2 cut(s)	75, 1440
BtsIMutI	CAGTG	6 cut(s)	75, 1410, 1440, 2172, 2905, 3143
BveI	ACCTGC	1 cut(s)	698
Cac8I	GCNNGC	3 cut(s)	43, 963, 1618
CciI	TCATGA	2 cut(s)	772, 2821
CfoI	GCGC	3 cut(s)	243, 1937, 2357
Cfr10I	RCCGGY	1 cut(s)	3184
Cfr13I	GGNCC	8 cut(s)	43, 44, 1216, 1235, 1274, 1654, 1699, 2925
ClaI	ATCGAT	1 cut(s)	1987
CseI	GACGC	2 cut(s)	123, 2339
CsiI	ACCWGGT	1 cut(s)	232
Csp6I	GTAC	4 cut(s)	178, 230, 1150, 3175
CspCI	CAANNNNNGTGG	2 cut(s)	1561, 1596
CviQI	GTAC	4 cut(s)	178, 230, 1150, 3175
DdeI	CTNAG	9 cut(s)	181, 209, 438, 655, 676, 796, 2162, 2561, 2589
DraI	TTTAAA	2 cut(s)	1902, 2026
DraIII	CACNNNGTG	1 cut(s)	1036
DrdI	GACNNNNNNGTC	2 cut(s)	1913, 2469
DseDI	GACNNNNNNGTC	2 cut(s)	1913, 2469
EaeI	YGGCCR	1 cut(s)	69
Eam1104I	CTCTTC	5 cut(s)	670, 1498, 1745, 2191, 2812
EarI	CTCTTC	5 cut(s)	670, 1498, 1745, 2191, 2812
Ecl136II	GAGCTC	1 cut(s)	486
Eco130I	CCWWGG	3 cut(s)	1186, 1277, 2106
Eco24I	GRGCYC	2 cut(s)	47, 488
Eco31I	GGTCTC	3 cut(s)	1203, 1409, 1978
Eco47I	GGWCC	4 cut(s)	1216, 1235, 1274, 1699
Eco53kI	GAGCTC	1 cut(s)	486
Eco57I	CTGAAG	3 cut(s)	372, 621, 1833
Eco72I	CACGTG	1 cut(s)	389
Eco81I	CCTNAGG	1 cut(s)	796
Eco88I	CYCGRG	2 cut(s)	220, 2439
Eco91I	GGTNACC	1 cut(s)	2760
EcoICRI	GAGCTC	1 cut(s)	486
EcoNI	CCTNNNNNAGG	1 cut(s)	256
EcoO109I	RGGNCCY	2 cut(s)	1216, 1699
EcoO65I	GGTNACC	1 cut(s)	2760
EcoRI	GAATTC	1 cut(s)	1248
EcoRII	CCWGG	6 cut(s)	232, 1379, 2383, 2419, 3037, 3067
EcoT14I	CCWWGG	3 cut(s)	1186, 1277, 2106
EcoT22I	ATGCAT	1 cut(s)	721
EcoT38I	GRGCYC	2 cut(s)	47, 488
ErhI	CCWWGG	3 cut(s)	1186, 1277, 2106
Esp3I	CGTCTC	1 cut(s)	2455
FalI	AAGNNNNNCTT	4 cut(s)	931, 963, 1548, 1580
FaqI	GGGAC	7 cut(s)	71, 644, 1041, 1202, 1685, 2380, 2799
FauI	CCCGC	1 cut(s)	34
FblI	GTMKAC	1 cut(s)	1534
FriOI	GRGCYC	2 cut(s)	47, 488
FspBI	CTAG	4 cut(s)	453, 1346, 2469, 2970
GlaI	GCGC	3 cut(s)	242, 1936, 2356
GsaI	CCCAGC	2 cut(s)	1308, 2501
GsuI	CTGGAG	1 cut(s)	3090
HaeII	RGCGCY	1 cut(s)	1938
HaeIII	GGCC	7 cut(s)	45, 71, 271, 393, 1656, 2171, 2927
HapII	CCGG	2 cut(s)	1140, 3185
HgaI	GACGC	2 cut(s)	123, 2339
HhaI	GCGC	3 cut(s)	243, 1937, 2357
Hin6I	GCGC	3 cut(s)	241, 1935, 2355
HinP1I	GCGC	3 cut(s)	241, 1935, 2355
HincII	GTYRAC	1 cut(s)	1535
HindII	GTYRAC	1 cut(s)	1535
HindIII	AAGCTT	2 cut(s)	1502, 1896
HinfI	GANTC	9 cut(s)	161, 416, 890, 914, 1391, 2224, 2232, 2804, 2935
HpaII	CCGG	2 cut(s)	1140, 3185
HphI	GGTGA	9 cut(s)	389, 515, 794, 842, 1016, 1154, 1559, 1872, 2442
Hpy99I	CGWCG	1 cut(s)	2355
HpyCH4III	ACNGT	8 cut(s)	534, 1034, 1414, 1636, 2150, 2655, 2900, 3138
HpyCH4IV	ACGT	2 cut(s)	388, 1729
HpyF10VI	GCNNNNNNNGC	8 cut(s)	32, 77, 1626, 1778, 2076, 2108, 2441, 3034
HpyF3I	CTNAG	9 cut(s)	181, 209, 438, 655, 676, 796, 2162, 2561, 2589
HpySE526I	ACGT	2 cut(s)	388, 1729
HspAI	GCGC	3 cut(s)	241, 1935, 2355
KflI	GGGWCCC	2 cut(s)	1216, 1699
LguI	GCTCTTC	1 cut(s)	1498
Lsp1109I	GCAGC	9 cut(s)	535, 952, 1457, 1570, 1936, 2091, 2094, 2322, 2717
LweI	GCATC	7 cut(s)	631, 706, 775, 1030, 1060, 2444, 2527
MabI	ACCWGGT	1 cut(s)	232
MaeI	CTAG	4 cut(s)	453, 1346, 2469, 2970
MaeII	ACGT	2 cut(s)	388, 1729
MaeIII	GTNAC	8 cut(s)	430, 973, 1129, 1142, 1294, 2320, 2760, 2947
MfeI	CAATTG	6 cut(s)	98, 1310, 1486, 2041, 2314, 2766
MflI	RGATCY	4 cut(s)	2276, 2423, 2599, 3082
MhlI	GDGCHC	6 cut(s)	47, 97, 488, 1370, 1380, 1572
MlsI	TGGCCA	1 cut(s)	71
MluNI	TGGCCA	1 cut(s)	71
MlyI	GAGTC	1 cut(s)	2798
MmeI	TCCRAC	1 cut(s)	844
Mox20I	TGGCCA	1 cut(s)	71
Mph1103I	ATGCAT	1 cut(s)	721
MroXI	GAANNNNTTC	4 cut(s)	165, 534, 871, 2571
MscI	TGGCCA	1 cut(s)	71
MslI	CAYNNNNRTG	5 cut(s)	543, 1122, 1373, 2633, 2945
Msp20I	TGGCCA	1 cut(s)	71
MspA1I	CMGCKG	2 cut(s)	1289, 1304
MspI	CCGG	2 cut(s)	1140, 3185
MspR9I	CCNGG	6 cut(s)	234, 1381, 2385, 2421, 3039, 3069
MunI	CAATTG	6 cut(s)	98, 1310, 1486, 2041, 2314, 2766
MvaI	CCWGG	6 cut(s)	234, 1381, 2385, 2421, 3039, 3069
MvnI	CGCG	1 cut(s)	2355
MwoI	GCNNNNNNNGC	8 cut(s)	32, 77, 1626, 1778, 2076, 2108, 2441, 3034
NcoI	CCATGG	2 cut(s)	1277, 2106
NlaIV	GGNNCC	9 cut(s)	45, 531, 1217, 1218, 1377, 1700, 1701, 2112, 2425
NmeAIII	GCCGAG	1 cut(s)	247
NmuCI	GTSAC	4 cut(s)	1129, 1142, 1294, 2947
NsiI	ATGCAT	1 cut(s)	721
NspI	RCATGY	3 cut(s)	965, 1107, 1150
NspV	TTCGAA	1 cut(s)	2933
OliI	CACNNNNGTG	2 cut(s)	1373, 2945
PaeI	GCATGC	1 cut(s)	965
PaeR7I	CTCGAG	2 cut(s)	220, 2439
PagI	TCATGA	2 cut(s)	772, 2821
PaqCI	CACCTGC	1 cut(s)	698
PasI	CCCWGGG	1 cut(s)	1380
PciI	ACATGT	1 cut(s)	1146
PciSI	GCTCTTC	1 cut(s)	1498
PcsI	WCGNNNNNNNCGW	1 cut(s)	3103
PdmI	GAANNNNTTC	4 cut(s)	165, 534, 871, 2571
PfeI	GAWTC	8 cut(s)	161, 416, 890, 914, 1391, 2224, 2232, 2935
PflMI	CCANNNNNTGG	1 cut(s)	2528
PfoI	TCCNGGA	1 cut(s)	2419
PleI	GAGTC	1 cut(s)	2798
PmaCI	CACGTG	1 cut(s)	389
PmlI	CACGTG	1 cut(s)	389
PpsI	GAGTC	1 cut(s)	2798
Ppu21I	YACGTR	1 cut(s)	389
PpuMI	RGGWCCY	2 cut(s)	1216, 1699
PscI	ACATGT	1 cut(s)	1146
PshBI	ATTAAT	1 cut(s)	380
PsiI	TTATAA	1 cut(s)	2049
Psp124BI	GAGCTC	1 cut(s)	488
Psp5II	RGGWCCY	2 cut(s)	1216, 1699
Psp6I	CCWGG	6 cut(s)	232, 1379, 2383, 2419, 3037, 3067
PspCI	CACGTG	1 cut(s)	389
PspEI	GGTNACC	1 cut(s)	2760
PspFI	CCCAGC	2 cut(s)	1304, 2497
PspGI	CCWGG	6 cut(s)	232, 1379, 2383, 2419, 3037, 3067
PspN4I	GGNNCC	9 cut(s)	45, 531, 1217, 1218, 1377, 1700, 1701, 2112, 2425
PspOMI	GGGCCC	1 cut(s)	43
PspPI	GGNCC	8 cut(s)	43, 44, 1216, 1235, 1274, 1654, 1699, 2925
PspPPI	RGGWCCY	2 cut(s)	1216, 1699
PstI	CTGCAG	1 cut(s)	1447
PsuI	RGATCY	4 cut(s)	2276, 2423, 2599, 3082
PvuII	CAGCTG	1 cut(s)	1289
RsaI	GTAC	4 cut(s)	179, 231, 1151, 3176
RsaNI	GTAC	4 cut(s)	178, 230, 1150, 3175
RseI	CAYNNNNRTG	5 cut(s)	543, 1122, 1373, 2633, 2945
SacI	GAGCTC	1 cut(s)	488
SalI	GTCGAC	1 cut(s)	1533
SapI	GCTCTTC	1 cut(s)	1498
Sau96I	GGNCC	8 cut(s)	43, 44, 1216, 1235, 1274, 1654, 1699, 2925
SchI	GAGTC	1 cut(s)	2798
ScrFI	CCNGG	6 cut(s)	234, 1381, 2385, 2421, 3039, 3069
SduI	GDGCHC	6 cut(s)	47, 97, 488, 1370, 1380, 1572
SexAI	ACCWGGT	1 cut(s)	232
SfaNI	GCATC	7 cut(s)	631, 706, 775, 1030, 1060, 2444, 2527
SfcI	CTRYAG	2 cut(s)	1443, 2835
Sfr274I	CTCGAG	2 cut(s)	220, 2439
SfuI	TTCGAA	1 cut(s)	2933
SinI	GGWCC	4 cut(s)	1216, 1235, 1274, 1699
SlaI	CTCGAG	2 cut(s)	220, 2439
SmiMI	CAYNNNNRTG	5 cut(s)	543, 1122, 1373, 2633, 2945
SmlI	CTYRAG	5 cut(s)	104, 220, 2096, 2439, 3086
SmoI	CTYRAG	5 cut(s)	104, 220, 2096, 2439, 3086
SphI	GCATGC	1 cut(s)	965
SsiI	CCGC	8 cut(s)	26, 41, 406, 1302, 1408, 1620, 3112, 3146
SspI	AATATT	1 cut(s)	1090
SspMI	CTAG	4 cut(s)	453, 1346, 2469, 2970
SstI	GAGCTC	1 cut(s)	488
StyD4I	CCNGG	6 cut(s)	232, 1379, 2383, 2419, 3037, 3067
StyI	CCWWGG	3 cut(s)	1186, 1277, 2106
TaaI	ACNGT	8 cut(s)	534, 1034, 1414, 1636, 2150, 2655, 2900, 3138
TaiI	ACGT	2 cut(s)	391, 1732
TatI	WGTACW	2 cut(s)	1149, 3174
TauI	GCSGC	1 cut(s)	3149
TfiI	GAWTC	8 cut(s)	161, 416, 890, 914, 1391, 2224, 2232, 2935
TscAI	CASTG	6 cut(s)	82, 1417, 1447, 2179, 2905, 3143
TseFI	GTSAC	4 cut(s)	1129, 1142, 1294, 2947
Tsp45I	GTSAC	4 cut(s)	1129, 1142, 1294, 2947
TspGWI	ACGGA	2 cut(s)	705, 2787
TspRI	CASTG	6 cut(s)	82, 1417, 1447, 2179, 2905, 3143
Van91I	CCANNNNNTGG	1 cut(s)	2528
VpaK11BI	GGWCC	4 cut(s)	1216, 1235, 1274, 1699
VspI	ATTAAT	1 cut(s)	380
XagI	CCTNNNNNAGG	1 cut(s)	256
XapI	RAATTY	6 cut(s)	131, 471, 624, 1248, 1874, 2866
XceI	RCATGY	3 cut(s)	965, 1107, 1150
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3208
XhoI	CTCGAG	2 cut(s)	220, 2439
XmiI	GTMKAC	1 cut(s)	1534
XmnI	GAANNNNTTC	4 cut(s)	165, 534, 871, 2571
XspI	CTAG	4 cut(s)	453, 1346, 2469, 2970
Zsp2I	ATGCAT	1 cut(s)	721

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.